Rroxscaffold_3G00232020

Patellin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012299
Physical Location & Seq
Reverse (-)
17590479 .. 17593417
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232020.1

Sequence Viewer

Length: 1623 bp
ATGGCTGATGAAACCCCCGTAACCACCCCGCCACCGGTAGACCAACCACCTTTCGCGGTGGTTGAGTCCGAATCGCCTTCTGTAGCCGAAAACCTCGAGTCTAAAGAAGACCCACTTCCACCAGCGCCATTTCCAGAAGTGATAGAGTCTGCCAAGGAAGAAGAGCCACCGCTAGAGCTGCCTCACGAGTCTCCTCCTGAGGTGAAGGCTAAATCCGAAGAGGAGGTTTTAGTGGTTGGGTCTGTGCAGGCCAAGGAGGGTGAGGAACCAAAGATTCGGAAACCGATTCCGCAGTCTCTGGTTTCGTTCAAAGAGGAAAGCAATATAGTGGCGGATCTCTCAGATTCCGAGAGGAAAGCACTTCAAGAACTGAAGCAGCTCGTCCAGGAAGCTCTCAACACTCGCCAGTTCACTTCCCCAAAACAAGAAGTGAAAGAGACCAAGGACAAACCAGTTCCCAAAACTGAAGAAGACACTGCTGGTTCGGAATCCGAAACAAAGCCGCTCCATGAGCCCAAAATCGAGGAGAACCCAGTGAAGGAAGCAGAGGAAGTAGCTAAGGAAACATCGCAACTGGCGATTTCTCCTGAGGAGGTTTCAATTTGGGGTGTTCCGCTGCTCAAGGACGACAGGAGCGATGTGATTCTTCTCAAGTTCCTGAGGGCTCGGGACTTCAAGGTGAGAGATGCTTTTGTTATGCTCCGAAACACGATCCAGTGGAGGAAGAAGTTTGGGATCGATGCTCTTGTGGACGAAGATCTCGGTGATGATTTGGAGAAAGTGGTGTTTATGCATGGGTATGACAGAGAGGGTCATCCTGTGTGTTACAATGTGTTTGGGGAGTTTCAGAACAAGGAGTTGTATCAGAAGACATTTTTGGATGAGGAAAAGAGAACCAAGTTTCTCCGGTGGCGGATTCAGTTCTTGGAGAGAAGCATTAGGAAGCTGGATTTCAGCCCTGGTGGTGTCTGCACTATTTTTCAGGTTAATGATCTTAAGAATTCTCCTGGCCCTGCAAAGAGTGAGCTTCGCTTGGCCACCAAACAAGCCCTGCAGTTGCTTCAGGACAATTATCCTGAGTTTGTAGCCAAACAGGTGTTTGTCAATGCTCCTTGGTGGTATCTTGCATTCTACACATTGATCAGTCCCTTCATGACACAGAGGACCAAAAGCAAGTTTGTCTTTGCAAGCCCAGCCAAATCTGCAGAGACCCTTTTCAAGTACATTTCTGCTGAGCATGTTCCTATACAATATGGGGGTTTGAGTGTGGATTACTGTGAGTGCAACCCAGAATTCACGATTGCTGATCCAGTCACTGATGTAATCATCAAACCTGGAACAAAGAAGACTGTGGAAATTATAATTTATGAGAAGTGCACCATCGTGTGGGAACTTCGTGTAGTTGGGTGGGAGGTGAGCTACAGTGCTGAGTTTGTTCCCGACTCTCAAGATGCATATACGCTGATCATTCAAAAAGCCACAAAGATGGCCCCAGCTGGTGAACCAGTGCTACTTCACAGGTTCAAAGTTGGCGAGCTGGGTAAAATATTGCTCACCATTGACAATCCAACCTCAAAGAAGAAGAGGCTTCTGTACAGGTTCAAGGTCGAACCCTCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

540

Amino Acids

61.39

Weight (kDa)

5.1

Isoelectric Point (pI)

56.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 185 - 236 1.1e-09 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 260 - 420 4.7e-31 CRAL/TRIO domain
GOLD_PATL1_C PF25099 444 - 528 6.5e-35 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012299)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72160
fragaria_vesca FvH4_5g31710 FvH4_5g31710
malus_domestica MD08G1218000.v1.1 MD15G1405900.v1.1
prunus_persica Prupe.1G555300_v2.0.a1
pyrus_communis pycom08g18900 pycom15g36270
rosa_chinensis RchiOBHm_Chr7g0227591
rosa_laevigata RLG00000001634
rosa_multiflora Rmu_sc0001772.1_g000008
rosa_roxburghii Rroxscaffold_3G00232020
rosa_rugosa Rorug07G0242000 Rorug07G0242100
rosa_samantha Rh7AG395500 Rh7BG377300 Rh7CG414200 Rh7DG391300
rosa_wichuraiana Rw7G032890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1361
AccB7I CCANNNNNTGG 1 cut(s) 1386
AccBSI CCGCTC 1 cut(s) 505
AccI GTMKAC 1 cut(s) 39
AccII CGCG 1 cut(s) 56
AciI CCGC 8 cut(s) 29, 56, 170, 290, 332, 503, 614, 913
AclWI GGATC 4 cut(s) 342, 706, 743, 1301
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 2 cut(s) 1000, 1292
AcuI CTGAAG 3 cut(s) 392, 486, 1046
AfaI GTAC 2 cut(s) 1223, 1595
AfiI CCNNNNNNNGG 3 cut(s) 34, 538, 1386
AflII CTTAAG 1 cut(s) 995
AgeI ACCGGT 1 cut(s) 34
AgsI TTSAA 8 cut(s) 310, 365, 600, 676, 1219, 1472, 1525, 1603
AjnI CCWGG 4 cut(s) 384, 958, 1006, 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AleI CACNNNNGTG 1 cut(s) 1382
AluBI AGCT 9 cut(s) 178, 379, 392, 557, 946, 1027, 1419, 1496, 1537
AluI AGCT 9 cut(s) 178, 379, 392, 557, 946, 1027, 1419, 1496, 1537
Alw21I GWGCWC 1 cut(s) 1379
Alw26I GTCTC 4 cut(s) 195, 300, 431, 1202
Alw44I GTGCAC 1 cut(s) 1375
AlwI GGATC 4 cut(s) 342, 706, 743, 1301
AlwNI CAGNNNCTG 2 cut(s) 298, 1316
Ama87I CYCGRG 2 cut(s) 95, 666
AoxI GGCC 4 cut(s) 249, 1009, 1035, 1488
ApaLI GTGCAC 1 cut(s) 1375
ApeKI GCWGC 3 cut(s) 178, 376, 616
ApoI RAATTY 2 cut(s) 1000, 1292
AsiGI ACCGGT 1 cut(s) 34
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 1010, 1164, 1489
AsuHPI GGTGA 7 cut(s) 214, 272, 691, 776, 1426, 1511, 1546
AvaI CYCGRG 2 cut(s) 95, 666
AvaII GGWCC 1 cut(s) 1164
AxyI CCTNAGG 3 cut(s) 198, 588, 659
BaeGI GKGCMC 1 cut(s) 1379
BalI TGGCCA 1 cut(s) 1037
BanII GRGCYC 2 cut(s) 516, 667
BauI CACGAG 1 cut(s) 185
BbsI GAAGAC 4 cut(s) 114, 477, 875, 1352
Bbv12I GWGCWC 1 cut(s) 1379
BbvI GCAGC 3 cut(s) 165, 388, 603
BccI CCATC 2 cut(s) 1388, 1480
BciT130I CCWGG 4 cut(s) 386, 960, 1008, 1335
BclI TGATCA 2 cut(s) 1140, 1464
BcoDI GTCTC 4 cut(s) 195, 300, 431, 1202
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 4 cut(s) 81, 1052, 1203, 1420
BfoI RGCGCY 1 cut(s) 128
BfrI CTTAAG 1 cut(s) 995
BglII AGATCT 1 cut(s) 757
BisI GCNGC 4 cut(s) 179, 377, 503, 617
BlpI GCTNAGC 1 cut(s) 1233
BlsI GCNGC 4 cut(s) 180, 378, 504, 618
Bme1390I CCNGG 4 cut(s) 386, 960, 1008, 1335
Bme18I GGWCC 1 cut(s) 1164
BmeT110I CYCGRG 2 cut(s) 95, 666
BmgT120I GGNCC 3 cut(s) 1010, 1164, 1489
BmiI GGNNCC 2 cut(s) 267, 1491
BmrFI CCNGG 4 cut(s) 386, 960, 1008, 1335
BmrI ACTGGG 1 cut(s) 527
BmsI GCATC 3 cut(s) 676, 730, 1441
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 4 cut(s) 114, 477, 875, 1352
Bpu10I CCTNAGC 1 cut(s) 558
Bpu1102I GCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 3 cut(s) 605, 635, 1431
Bsa29I ATCGAT 1 cut(s) 738
BsaI GGTCTC 2 cut(s) 431, 1202
BsaJI CCNNGG 5 cut(s) 153, 252, 441, 958, 1112
BsaWI WCCGGW 2 cut(s) 34, 906
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 538, 1386
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 7 cut(s) 406, 452, 533, 579, 715, 1310, 1505
Bse21I CCTNAGG 3 cut(s) 198, 588, 659
BseBI CCWGG 4 cut(s) 386, 960, 1008, 1335
BseCI ATCGAT 1 cut(s) 738
BseDI CCNNGG 5 cut(s) 153, 252, 441, 958, 1112
BseGI GGATG 2 cut(s) 814, 886
BseLI CCNNNNNNNGG 3 cut(s) 34, 538, 1386
BseMII CTCAG 7 cut(s) 189, 354, 579, 650, 1068, 1224, 1419
BseNI ACTGG 7 cut(s) 406, 452, 533, 579, 715, 1310, 1505
BseRI GAGGAG 4 cut(s) 183, 236, 539, 605
BseSI GKGCMC 1 cut(s) 1379
BseXI GCAGC 3 cut(s) 165, 388, 603
BseYI CCCAGC 3 cut(s) 1192, 1492, 1537
BsgI GTGCAG 2 cut(s) 266, 955
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 4 cut(s) 251, 1011, 1037, 1490
BshTI ACCGGT 1 cut(s) 34
BshVI ATCGAT 1 cut(s) 738
BsiHKAI GWGCWC 1 cut(s) 1379
BsiHKCI CYCGRG 2 cut(s) 95, 666
BsiSI CCGG 2 cut(s) 35, 907
BslFI GGGAC 2 cut(s) 683, 1131
BslI CCNNNNNNNGG 3 cut(s) 34, 538, 1386
BsmAI GTCTC 4 cut(s) 195, 300, 431, 1202
BsmFI GGGAC 2 cut(s) 683, 1131
BsmI GAATGC 1 cut(s) 1127
BsnI GGCC 4 cut(s) 251, 1011, 1037, 1490
Bso31I GGTCTC 2 cut(s) 431, 1202
BsoBI CYCGRG 2 cut(s) 95, 666
Bsp1286I GDGCHC 3 cut(s) 516, 667, 1379
Bsp1407I TGTACA 1 cut(s) 1593
Bsp143I GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
Bsp1720I GCTNAGC 1 cut(s) 1233
BspACI CCGC 8 cut(s) 29, 56, 170, 290, 332, 503, 614, 913
BspANI GGCC 4 cut(s) 251, 1011, 1037, 1490
BspCNI CTCAG 7 cut(s) 190, 353, 580, 651, 1069, 1225, 1420
BspDI ATCGAT 1 cut(s) 738
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 2 cut(s) 267, 1491
BspMAI CTGCAG 2 cut(s) 1056, 1207
BspPI GGATC 4 cut(s) 342, 706, 743, 1301
BspQI GCTCTTC 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 995
BspTNI GGTCTC 2 cut(s) 431, 1202
BsrBI CCGCTC 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 1593
BsrI ACTGG 7 cut(s) 406, 452, 533, 579, 715, 1310, 1505
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 5 cut(s) 153, 252, 441, 958, 1112
BssMI GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 4 cut(s) 153, 252, 441, 1112
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 4 cut(s) 386, 960, 1008, 1335
Bst4CI ACNGT 3 cut(s) 1277, 1351, 1424
Bst6I CTCTTC 3 cut(s) 156, 213, 1577
BstAFI CTTAAG 1 cut(s) 995
BstAUI TGTACA 1 cut(s) 1593
BstC8I GCNNGC 3 cut(s) 249, 1189, 1535
BstDEI CTNAG 8 cut(s) 198, 340, 558, 588, 659, 1077, 1233, 1428
BstF5I GGATG 2 cut(s) 814, 886
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 8 cut(s) 337, 714, 738, 760, 994, 1143, 1309, 1467
BstMAI GTCTC 4 cut(s) 195, 300, 431, 1202
BstMBI GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
BstMWI GCNNNNNNNGC 4 cut(s) 178, 511, 1193, 1202
BstNI CCWGG 4 cut(s) 386, 960, 1008, 1335
BstNSI RCATGY 1 cut(s) 1241
BstSCI CCNGG 4 cut(s) 384, 958, 1006, 1333
BstSFI CTRYAG 4 cut(s) 81, 1052, 1203, 1420
BstSLI GKGCMC 1 cut(s) 1379
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 3 cut(s) 165, 388, 603
BstV2I GAAGAC 4 cut(s) 114, 477, 875, 1352
BstX2I RGATCY 2 cut(s) 334, 757
BstXI CCANNNNNNTGG 1 cut(s) 1486
BstYI RGATCY 2 cut(s) 334, 757
Bsu15I ATCGAT 1 cut(s) 738
Bsu36I CCTNAGG 3 cut(s) 198, 588, 659
BsuRI GGCC 4 cut(s) 251, 1011, 1037, 1490
BsuTUI ATCGAT 1 cut(s) 738
BtgZI GCGATG 2 cut(s) 552, 651
BtsCI GGATG 2 cut(s) 814, 886
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 6 cut(s) 474, 540, 722, 1314, 1429, 1512
Cac8I GCNNGC 3 cut(s) 249, 1189, 1535
CaiI CAGNNNCTG 2 cut(s) 298, 1316
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 1 cut(s) 127
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 1010, 1164, 1489
ClaI ATCGAT 1 cut(s) 738
Csp6I GTAC 2 cut(s) 1222, 1594
CspAI ACCGGT 1 cut(s) 34
CviAII CATG 4 cut(s) 509, 794, 1153, 1238
CviQI GTAC 2 cut(s) 1222, 1594
DdeI CTNAG 8 cut(s) 198, 340, 558, 588, 659, 1077, 1233, 1428
DpnI GATC 8 cut(s) 336, 713, 737, 759, 993, 1142, 1308, 1466
DpnII GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 3 cut(s) 156, 213, 1577
EarI CTCTTC 3 cut(s) 156, 213, 1577
EciI GGCGGA 2 cut(s) 347, 928
Eco130I CCWWGG 4 cut(s) 153, 252, 441, 1112
Eco24I GRGCYC 2 cut(s) 516, 667
Eco31I GGTCTC 2 cut(s) 431, 1202
Eco47I GGWCC 1 cut(s) 1164
Eco57I CTGAAG 3 cut(s) 392, 486, 1046
Eco81I CCTNAGG 3 cut(s) 198, 588, 659
Eco88I CYCGRG 2 cut(s) 95, 666
EcoRI GAATTC 2 cut(s) 1000, 1292
EcoRII CCWGG 4 cut(s) 384, 958, 1006, 1333
EcoT14I CCWWGG 4 cut(s) 153, 252, 441, 1112
EcoT22I ATGCAT 2 cut(s) 795, 1456
EcoT38I GRGCYC 2 cut(s) 516, 667
ErhI CCWWGG 4 cut(s) 153, 252, 441, 1112
FaeI CATG 4 cut(s) 512, 797, 1156, 1241
FalI AAGNNNNNCTT 4 cut(s) 99, 131, 1166, 1198
FaqI GGGAC 2 cut(s) 683, 1131
FatI CATG 4 cut(s) 508, 793, 1152, 1237
FauI CCCGC 1 cut(s) 36
FbaI TGATCA 2 cut(s) 1140, 1464
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 4 cut(s) 179, 377, 503, 617
FokI GGATG 2 cut(s) 801, 893
FriOI GRGCYC 2 cut(s) 516, 667
Fsp4HI GCNGC 4 cut(s) 179, 377, 503, 617
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 126
GluI GCNGC 4 cut(s) 179, 377, 503, 617
GsaI CCCAGC 3 cut(s) 1196, 1496, 1541
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 4 cut(s) 251, 1011, 1037, 1490
HapII CCGG 2 cut(s) 35, 907
HhaI GCGC 1 cut(s) 127
Hin1II CATG 4 cut(s) 512, 797, 1156, 1241
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HpaII CCGG 2 cut(s) 35, 907
HphI GGTGA 7 cut(s) 214, 272, 691, 776, 1426, 1511, 1546
Hpy166II GTNNAC 5 cut(s) 40, 411, 751, 1377, 1502
Hpy8I GTNNAC 5 cut(s) 40, 411, 751, 1377, 1502
HpyAV CCTTC 4 cut(s) 87, 199, 532, 1159
HpyCH4III ACNGT 3 cut(s) 1277, 1351, 1424
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 511, 1193, 1202
HpyF3I CTNAG 8 cut(s) 198, 340, 558, 588, 659, 1077, 1233, 1428
Hsp92II CATG 4 cut(s) 512, 797, 1156, 1241
HspAI GCGC 1 cut(s) 125
Ksp22I TGATCA 2 cut(s) 1140, 1464
Kzo9I GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 4 cut(s) 510, 633, 705, 1114
Lsp1109I GCAGC 3 cut(s) 165, 388, 603
LweI GCATC 3 cut(s) 676, 730, 1441
MaeI CTAG 1 cut(s) 173
MaeIII GTNAC 3 cut(s) 19, 824, 1312
MalI GATC 8 cut(s) 336, 713, 737, 759, 993, 1142, 1308, 1466
MbiI CCGCTC 1 cut(s) 505
MboI GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
MflI RGATCY 2 cut(s) 334, 757
MhlI GDGCHC 3 cut(s) 516, 667, 1379
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 6 cut(s) 600, 1000, 1069, 1292, 1356, 1362
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 5 cut(s) 74, 107, 155, 197, 1436
MmeI TCCRAC 1 cut(s) 1592
Mox20I TGGCCA 1 cut(s) 1037
Mph1103I ATGCAT 2 cut(s) 795, 1456
MscI TGGCCA 1 cut(s) 1037
MseI TTAA 2 cut(s) 987, 996
MslI CAYNNNNRTG 3 cut(s) 798, 1382, 1484
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 2 cut(s) 616, 1496
MspCI CTTAAG 1 cut(s) 995
MspI CCGG 2 cut(s) 35, 907
MspR9I CCNGG 4 cut(s) 386, 960, 1008, 1335
Mva1269I GAATGC 1 cut(s) 1127
MvaI CCWGG 4 cut(s) 386, 960, 1008, 1335
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 4 cut(s) 178, 511, 1193, 1202
NdeII GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
NlaIII CATG 4 cut(s) 512, 797, 1156, 1241
NlaIV GGNNCC 2 cut(s) 267, 1491
NmuCI GTSAC 1 cut(s) 1312
NsiI ATGCAT 2 cut(s) 795, 1456
NspI RCATGY 1 cut(s) 1241
OliI CACNNNNGTG 1 cut(s) 1382
PaeR7I CTCGAG 1 cut(s) 95
PagI TCATGA 1 cut(s) 1152
PciSI GCTCTTC 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 575, 633
PctI GAATGC 1 cut(s) 1127
PfeI GAWTC 7 cut(s) 71, 274, 286, 344, 488, 643, 916
PflMI CCANNNNNTGG 1 cut(s) 1386
PfoI TCCNGGA 1 cut(s) 384
PinAI ACCGGT 1 cut(s) 34
PkrI GCNGC 4 cut(s) 180, 378, 504, 618
PleI GAGTC 5 cut(s) 73, 106, 154, 196, 1436
PpsI GAGTC 5 cut(s) 73, 106, 154, 196, 1436
PsiI TTATAA 1 cut(s) 1361
Psp6I CCWGG 4 cut(s) 384, 958, 1006, 1333
PspFI CCCAGC 3 cut(s) 1192, 1492, 1537
PspGI CCWGG 4 cut(s) 384, 958, 1006, 1333
PspN4I GGNNCC 2 cut(s) 267, 1491
PspPI GGNCC 3 cut(s) 1010, 1164, 1489
PspXI VCTCGAGB 1 cut(s) 95
PsrI GAACNNNNNNTAC 2 cut(s) 1494, 1526
PstI CTGCAG 2 cut(s) 1056, 1207
PstNI CAGNNNCTG 2 cut(s) 298, 1316
PsuI RGATCY 2 cut(s) 334, 757
PvuII CAGCTG 1 cut(s) 1496
RsaI GTAC 2 cut(s) 1223, 1595
RsaNI GTAC 2 cut(s) 1222, 1594
RseI CAYNNNNRTG 3 cut(s) 798, 1382, 1484
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 2 cut(s) 987, 996
SatI GCNGC 4 cut(s) 179, 377, 503, 617
Sau3AI GATC 8 cut(s) 334, 711, 735, 757, 991, 1140, 1306, 1464
Sau96I GGNCC 3 cut(s) 1010, 1164, 1489
SchI GAGTC 5 cut(s) 74, 107, 155, 197, 1436
ScrFI CCNGG 4 cut(s) 386, 960, 1008, 1335
SduI GDGCHC 3 cut(s) 516, 667, 1379
SfaNI GCATC 3 cut(s) 676, 730, 1441
SfcI CTRYAG 4 cut(s) 81, 1052, 1203, 1420
Sfr274I CTCGAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 1164
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 3 cut(s) 798, 1382, 1484
SmlI CTYRAG 5 cut(s) 95, 620, 650, 995, 1446
SmoI CTYRAG 5 cut(s) 95, 620, 650, 995, 1446
Sse9I AATT 6 cut(s) 600, 1000, 1069, 1292, 1356, 1362
SsiI CCGC 8 cut(s) 29, 56, 170, 290, 332, 503, 614, 913
SspI AATATT 1 cut(s) 1548
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 4 cut(s) 384, 958, 1006, 1333
StyI CCWWGG 4 cut(s) 153, 252, 441, 1112
TaaI ACNGT 3 cut(s) 1277, 1351, 1424
TaqI TCGA 4 cut(s) 96, 522, 738, 1608
TasI AATT 6 cut(s) 600, 1000, 1069, 1292, 1356, 1362
TatI WGTACW 2 cut(s) 1221, 1593
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 7 cut(s) 71, 274, 286, 344, 488, 643, 916
Tru1I TTAA 2 cut(s) 987, 996
Tru9I TTAA 2 cut(s) 987, 996
TscAI CASTG 6 cut(s) 481, 540, 722, 1321, 1429, 1512
TseFI GTSAC 1 cut(s) 1312
TseI GCWGC 3 cut(s) 178, 376, 616
Tsp45I GTSAC 1 cut(s) 1312
TspDTI ATGAA 2 cut(s) 24, 1141
TspRI CASTG 6 cut(s) 481, 540, 722, 1321, 1429, 1512
Van91I CCANNNNNTGG 1 cut(s) 1386
Vha464I CTTAAG 1 cut(s) 995
VneI GTGCAC 1 cut(s) 1375
VpaK11BI GGWCC 1 cut(s) 1164
XapI RAATTY 2 cut(s) 1000, 1292
XceI RCATGY 1 cut(s) 1241
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 1 cut(s) 173
Zsp2I ATGCAT 2 cut(s) 795, 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.