AT1G73060

low psii accumulation 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008650
Physical Location & Seq
Forward (+)
27478970 .. 27481561
2592 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73060.1

Sequence Viewer

Length: 1077 bp
ATGGCTATGGCGATTGCGATGTTACCGGCGCTCTTCTCTTCTCCTTCGATTCTAACCAGCAGAATCAGATGCGGAGCCACCGCAAACAGCGGCGGCGCAATCTCATCTACTTCTTCTAATTCCGATCCTAGGAGAGGTGTTCCCTTGTATAAGCCCAAATCATACGAGGTTCTAGCCACTGACGCCGCCAATTCTCTAGCTTTCGCTCTCCAGGATTCAAAATCTCGTTTGGAAATCGATTTTCCACCTTTACCCAGTAGCATTTCTTCTTACAAGGGTTCTTCAGACGACTTTATTGATGCTAATATACAACTGGCCGTGACTGTTGTTCGCAAACTTCAGGAGAAAATCGAGACCAGAGCATGCATTGTTTTTCCTGACAAGCCTGAGAAGCGCAGGGCTTCGCAACGCTTCAAAGCGGCATTTGATTCGGTTGATGGCATAAGTATTGGCTCTCTAGATGATATACCTGGTACTTCTGTCACCAATTTCTTTAGGTCCATAAGGAGTACCCTGGACTTTGATTTTGAAGATGAAAACGAAGGAACTTGGGAGCCCAAGGAGCCACCGACCCTTTATATATTTATCAATTGCAGCACTCGGGAACTCTCTTTCATAGAGAAGTTTGTGGAAACCTTTGCCAGTTCAACCCCAGCTCTTCTGTTCAATCTTGAGCTAGATACTTTGCGTGCTGACCTAGGGCTTCTTGGCTTTCCCCCTAAAGATCTGCACTACCGGTTTCTTTCTCAATTTATTCCCGTCTTTTATATCCGAACCAGAGAGTATTCAAAGACAGTGGCAGTGGCACCATTTGTGTTGAACTACAACGGAGCACTTTTTCGACAATATCCTGGGCCATGGCAGGTGATGCTTAAGCAAACTGATGGTTCATTTGCGTGTGTTGCAGAAAGCCCTACTCGTTTCACGCTGGGTGAAACGAAGGAAGAGCTGTTACAGGTTCTGGGACTACAAGAAGAGAAAGGAAGCTCACTCGAGTTCCTGCGCAGGGGATACAAGTCGGCAACTTGGTGGGAAGAGGATGTGGAGCTCGAGGCATCATCTAATTGGCGTAACTGA

Protein Analysis

358

Amino Acids

40.03

Weight (kDa)

5.12

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 52 - 314 8.4e-47 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 853
Acc16I TGCGCA 1 cut(s) 1004
Acc36I ACCTGC 1 cut(s) 853
AccB1I GGYRCC 1 cut(s) 805
AciI CCGC 6 cut(s) 72, 81, 90, 93, 186, 419
AclWI GGATC 1 cut(s) 119
AcoI YGGCCR 1 cut(s) 315
AcuI CTGAAG 2 cut(s) 267, 323
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 2 cut(s) 475, 511
AfiI CCNNNNNNNGG 3 cut(s) 129, 134, 1006
AflII CTTAAG 1 cut(s) 872
AgeI ACCGGT 1 cut(s) 735
AgsI TTSAA 7 cut(s) 219, 415, 530, 648, 667, 789, 820
AjnI CCWGG 4 cut(s) 210, 469, 513, 850
AluBI AGCT 6 cut(s) 200, 656, 676, 949, 987, 1048
AluI AGCT 6 cut(s) 200, 656, 676, 949, 987, 1048
Alw21I GWGCWC 2 cut(s) 835, 1050
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 119
AlwNI CAGNNNCTG 1 cut(s) 961
Ama87I CYCGRG 3 cut(s) 600, 992, 1049
AoxI GGCC 2 cut(s) 315, 854
ApeKI GCWGC 1 cut(s) 594
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AsiGI ACCGGT 1 cut(s) 735
AspA2I CCTAGG 2 cut(s) 128, 697
AspLEI GCGC 4 cut(s) 31, 98, 396, 1005
AspS9I GGNCC 2 cut(s) 498, 854
AsuHPI GGTGA 3 cut(s) 475, 877, 944
AvaI CYCGRG 3 cut(s) 600, 992, 1049
AvaII GGWCC 1 cut(s) 498
AvrII CCTAGG 2 cut(s) 128, 697
BaeI ACNNNNGTAYC 2 cut(s) 465, 498
BanI GGYRCC 1 cut(s) 805
BanII GRGCYC 2 cut(s) 558, 1050
BarI GAAGNNNNNNTAC 4 cut(s) 250, 282, 936, 968
Bbv12I GWGCWC 2 cut(s) 835, 1050
BbvI GCAGC 1 cut(s) 606
BccI CCATC 2 cut(s) 431, 878
BceAI ACGGC 1 cut(s) 302
BcgI CGANNNNNNTGC 2 cut(s) 411, 445
BciT130I CCWGG 4 cut(s) 212, 471, 515, 852
BciVI GTATCC 1 cut(s) 1004
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 6 cut(s) 129, 173, 197, 458, 677, 698
BfoI RGCGCY 1 cut(s) 32
BfrI CTTAAG 1 cut(s) 872
BfuAI ACCTGC 1 cut(s) 853
BfuI GTATCC 1 cut(s) 1004
BglII AGATCT 1 cut(s) 724
BisI GCNGC 5 cut(s) 91, 94, 186, 420, 595
BlnI CCTAGG 2 cut(s) 128, 697
BlsI GCNGC 5 cut(s) 92, 95, 187, 421, 596
Bme1390I CCNGG 4 cut(s) 212, 471, 515, 852
Bme18I GGWCC 1 cut(s) 498
BmeT110I CYCGRG 3 cut(s) 600, 992, 1049
BmgT120I GGNCC 2 cut(s) 498, 854
BmiI GGNNCC 4 cut(s) 76, 555, 564, 807
BmrFI CCNGG 4 cut(s) 212, 471, 515, 852
BmrI ACTGGG 1 cut(s) 249
BmsI GCATC 4 cut(s) 59, 289, 858, 1064
BmuI ACTGGG 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 6 cut(s) 128, 513, 558, 697, 851, 857
BsaWI WCCGGW 1 cut(s) 735
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 134, 1006
Bse118I RCCGGY 2 cut(s) 25, 735
Bse1I ACTGG 3 cut(s) 255, 318, 642
BseBI CCWGG 4 cut(s) 212, 471, 515, 852
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 6 cut(s) 128, 513, 558, 697, 851, 857
BseGI GGATG 1 cut(s) 1045
BseLI CCNNNNNNNGG 3 cut(s) 129, 134, 1006
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 3 cut(s) 255, 318, 642
BseXI GCAGC 1 cut(s) 606
BseYI CCCAGC 2 cut(s) 652, 928
BsgI GTGCAG 1 cut(s) 713
BshFI GGCC 2 cut(s) 317, 856
BshNI GGYRCC 1 cut(s) 805
BshTI ACCGGT 1 cut(s) 735
BshVI ATCGAT 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 835, 1050
BsiHKCI CYCGRG 3 cut(s) 600, 992, 1049
BsiSI CCGG 2 cut(s) 26, 736
BslFI GGGAC 1 cut(s) 978
BslI CCNNNNNNNGG 3 cut(s) 129, 134, 1006
BsmAI GTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 978
BsnI GGCC 2 cut(s) 317, 856
Bso31I GGTCTC 1 cut(s) 347
BsoBI CYCGRG 3 cut(s) 600, 992, 1049
Bsp1286I GDGCHC 3 cut(s) 558, 835, 1050
Bsp143I GATC 2 cut(s) 124, 724
Bsp19I CCATGG 1 cut(s) 857
BspACI CCGC 6 cut(s) 72, 81, 90, 93, 186, 419
BspANI GGCC 2 cut(s) 317, 856
BspCNI CTCAG 1 cut(s) 379
BspDI ATCGAT 1 cut(s) 237
BspLI GGNNCC 4 cut(s) 76, 555, 564, 807
BspMI ACCTGC 1 cut(s) 853
BspPI GGATC 1 cut(s) 119
BspQI GCTCTTC 3 cut(s) 38, 663, 939
BspT107I GGYRCC 1 cut(s) 805
BspTI CTTAAG 1 cut(s) 872
BspTNI GGTCTC 1 cut(s) 347
BsrFI RCCGGY 2 cut(s) 25, 735
BsrI ACTGG 3 cut(s) 255, 318, 642
BssAI RCCGGY 2 cut(s) 25, 735
BssECI CCNNGG 6 cut(s) 128, 513, 558, 697, 851, 857
BssMI GATC 2 cut(s) 124, 724
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 4 cut(s) 128, 558, 697, 857
Bst2UI CCWGG 4 cut(s) 212, 471, 515, 852
Bst4CI ACNGT 2 cut(s) 325, 796
Bst6I CTCTTC 6 cut(s) 38, 43, 663, 939, 969, 1029
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 872
BstAPI GCANNNNNTGC 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 364, 690
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 857
BstENI CCTNNNNNAGG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 1045
BstH2I RGCGCY 1 cut(s) 32
BstHHI GCGC 4 cut(s) 31, 98, 396, 1005
BstKTI GATC 2 cut(s) 127, 727
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 2 cut(s) 124, 724
BstMWI GCNNNNNNNGC 5 cut(s) 182, 391, 562, 868, 902
BstNI CCWGG 4 cut(s) 212, 471, 515, 852
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 4 cut(s) 210, 469, 513, 850
BstV1I GCAGC 1 cut(s) 606
BstX2I RGATCY 1 cut(s) 724
BstYI RGATCY 1 cut(s) 724
Bsu15I ATCGAT 1 cut(s) 237
BsuI GTATCC 1 cut(s) 1004
BsuRI GGCC 2 cut(s) 317, 856
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 857
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 1 cut(s) 1045
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 3 cut(s) 177, 801, 807
BveI ACCTGC 1 cut(s) 853
Cac8I GCNNGC 2 cut(s) 364, 690
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 4 cut(s) 31, 98, 396, 1005
Cfr10I RCCGGY 2 cut(s) 25, 735
Cfr13I GGNCC 2 cut(s) 498, 854
ClaI ATCGAT 1 cut(s) 237
CseI GACGC 1 cut(s) 191
CsiI ACCWGGT 1 cut(s) 469
Csp6I GTAC 2 cut(s) 474, 510
CspAI ACCGGT 1 cut(s) 735
CspCI CAANNNNNGTGG 2 cut(s) 777, 812
CviAII CATG 2 cut(s) 363, 858
CviQI GTAC 2 cut(s) 474, 510
DdeI CTNAG 1 cut(s) 387
DpnI GATC 2 cut(s) 126, 726
DpnII GATC 2 cut(s) 124, 724
EaeI YGGCCR 1 cut(s) 315
Eam1104I CTCTTC 6 cut(s) 38, 43, 663, 939, 969, 1029
EarI CTCTTC 6 cut(s) 38, 43, 663, 939, 969, 1029
Ecl136II GAGCTC 1 cut(s) 1048
Eco130I CCWWGG 4 cut(s) 128, 558, 697, 857
Eco24I GRGCYC 2 cut(s) 558, 1050
Eco31I GGTCTC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 1048
Eco57I CTGAAG 2 cut(s) 267, 323
Eco88I CYCGRG 3 cut(s) 600, 992, 1049
EcoICRI GAGCTC 1 cut(s) 1048
EcoNI CCTNNNNNAGG 1 cut(s) 132
EcoRII CCWGG 4 cut(s) 210, 469, 513, 850
EcoT14I CCWWGG 4 cut(s) 128, 558, 697, 857
EcoT22I ATGCAT 1 cut(s) 368
EcoT38I GRGCYC 2 cut(s) 558, 1050
ErhI CCWWGG 4 cut(s) 128, 558, 697, 857
FaeI CATG 2 cut(s) 366, 861
FaqI GGGAC 1 cut(s) 978
FatI CATG 2 cut(s) 362, 857
Fnu4HI GCNGC 5 cut(s) 91, 94, 186, 420, 595
FokI GGATG 1 cut(s) 1052
FriOI GRGCYC 2 cut(s) 558, 1050
Fsp4HI GCNGC 5 cut(s) 91, 94, 186, 420, 595
FspBI CTAG 6 cut(s) 129, 173, 197, 458, 677, 698
FspI TGCGCA 1 cut(s) 1004
GlaI GCGC 4 cut(s) 30, 97, 395, 1004
GluI GCNGC 5 cut(s) 91, 94, 186, 420, 595
GsaI CCCAGC 2 cut(s) 656, 932
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 32
HaeIII GGCC 2 cut(s) 317, 856
HapII CCGG 2 cut(s) 26, 736
HgaI GACGC 1 cut(s) 191
HhaI GCGC 4 cut(s) 31, 98, 396, 1005
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 2 cut(s) 366, 861
Hin6I GCGC 4 cut(s) 29, 96, 394, 1003
HinP1I GCGC 4 cut(s) 29, 96, 394, 1003
HinfI GANTC 4 cut(s) 49, 63, 215, 428
HpaII CCGG 2 cut(s) 26, 736
HphI GGTGA 3 cut(s) 475, 877, 944
Hpy188I TCNGA 4 cut(s) 68, 124, 286, 773
Hpy188III TCNNGA 6 cut(s) 341, 352, 377, 458, 602, 671
HpyAV CCTTC 3 cut(s) 54, 536, 934
HpyCH4III ACNGT 2 cut(s) 325, 796
HpyCH4V TGCA 4 cut(s) 366, 594, 730, 905
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 391, 562, 868, 902
HpyF3I CTNAG 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 2 cut(s) 366, 861
HspAI GCGC 4 cut(s) 29, 96, 394, 1003
Kzo9I GATC 2 cut(s) 124, 724
LguI GCTCTTC 3 cut(s) 38, 663, 939
LmnI GCTCC 5 cut(s) 74, 553, 562, 830, 1045
Lsp1109I GCAGC 1 cut(s) 606
LweI GCATC 4 cut(s) 59, 289, 858, 1064
MabI ACCWGGT 1 cut(s) 469
MaeI CTAG 6 cut(s) 129, 173, 197, 458, 677, 698
MaeIII GTNAC 5 cut(s) 21, 319, 481, 951, 1070
MalI GATC 2 cut(s) 126, 726
MboI GATC 2 cut(s) 124, 724
MfeI CAATTG 1 cut(s) 589
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 3 cut(s) 558, 835, 1050
MluCI AATT 6 cut(s) 118, 190, 487, 589, 749, 1063
MnlI CCTC 4 cut(s) 128, 160, 1030, 1045
Mph1103I ATGCAT 1 cut(s) 368
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 1 cut(s) 895
MspA1I CMGCKG 1 cut(s) 90
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 2 cut(s) 26, 736
MspR9I CCNGG 4 cut(s) 212, 471, 515, 852
MunI CAATTG 1 cut(s) 589
MvaI CCWGG 4 cut(s) 212, 471, 515, 852
MwoI GCNNNNNNNGC 5 cut(s) 182, 391, 562, 868, 902
NcoI CCATGG 1 cut(s) 857
NdeII GATC 2 cut(s) 124, 724
NlaIII CATG 2 cut(s) 366, 861
NlaIV GGNNCC 4 cut(s) 76, 555, 564, 807
NmuCI GTSAC 2 cut(s) 319, 481
NsbI TGCGCA 1 cut(s) 1004
NsiI ATGCAT 1 cut(s) 368
NspI RCATGY 1 cut(s) 366
PaeI GCATGC 1 cut(s) 366
PaeR7I CTCGAG 2 cut(s) 992, 1049
PaqCI CACCTGC 1 cut(s) 853
PciSI GCTCTTC 3 cut(s) 38, 663, 939
PfeI GAWTC 4 cut(s) 49, 63, 215, 428
PfoI TCCNGGA 1 cut(s) 210
PinAI ACCGGT 1 cut(s) 735
PkrI GCNGC 5 cut(s) 92, 95, 187, 421, 596
Psp124BI GAGCTC 1 cut(s) 1050
Psp6I CCWGG 4 cut(s) 210, 469, 513, 850
PspFI CCCAGC 2 cut(s) 652, 928
PspGI CCWGG 4 cut(s) 210, 469, 513, 850
PspN4I GGNNCC 4 cut(s) 76, 555, 564, 807
PspPI GGNCC 2 cut(s) 498, 854
PspXI VCTCGAGB 2 cut(s) 992, 1049
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 1 cut(s) 724
RsaI GTAC 2 cut(s) 475, 511
RsaNI GTAC 2 cut(s) 474, 510
RseI CAYNNNNRTG 1 cut(s) 895
SacI GAGCTC 1 cut(s) 1050
SapI GCTCTTC 3 cut(s) 38, 663, 939
SaqAI TTAA 1 cut(s) 873
SatI GCNGC 5 cut(s) 91, 94, 186, 420, 595
Sau3AI GATC 2 cut(s) 124, 724
Sau96I GGNCC 2 cut(s) 498, 854
ScrFI CCNGG 4 cut(s) 212, 471, 515, 852
SduI GDGCHC 3 cut(s) 558, 835, 1050
SexAI ACCWGGT 1 cut(s) 469
SfaNI GCATC 4 cut(s) 59, 289, 858, 1064
Sfr274I CTCGAG 2 cut(s) 992, 1049
SinI GGWCC 1 cut(s) 498
SlaI CTCGAG 2 cut(s) 992, 1049
SmiMI CAYNNNNRTG 1 cut(s) 895
SmlI CTYRAG 4 cut(s) 671, 872, 992, 1049
SmoI CTYRAG 4 cut(s) 671, 872, 992, 1049
SphI GCATGC 1 cut(s) 366
Sse9I AATT 6 cut(s) 118, 190, 487, 589, 749, 1063
SsiI CCGC 6 cut(s) 72, 81, 90, 93, 186, 419
SspMI CTAG 6 cut(s) 129, 173, 197, 458, 677, 698
SstI GAGCTC 1 cut(s) 1050
StyD4I CCNGG 4 cut(s) 210, 469, 513, 850
StyI CCWWGG 4 cut(s) 128, 558, 697, 857
TaaI ACNGT 2 cut(s) 325, 796
TaqI TCGA 6 cut(s) 47, 237, 351, 841, 993, 1050
TasI AATT 6 cut(s) 118, 190, 487, 589, 749, 1063
TauI GCSGC 4 cut(s) 93, 96, 188, 422
TfiI GAWTC 4 cut(s) 49, 63, 215, 428
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 3 cut(s) 184, 801, 807
TseFI GTSAC 2 cut(s) 319, 481
TseI GCWGC 1 cut(s) 594
Tsp45I GTSAC 2 cut(s) 319, 481
TspDTI ATGAA 3 cut(s) 549, 604, 879
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 3 cut(s) 184, 801, 807
Vha464I CTTAAG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 498
XagI CCTNNNNNAGG 1 cut(s) 132
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 1 cut(s) 366
XhoI CTCGAG 2 cut(s) 992, 1049
XmaJI CCTAGG 2 cut(s) 128, 697
XspI CTAG 6 cut(s) 129, 173, 197, 458, 677, 698
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.