MD10G1047600.v1.1

Protein LOW PSII ACCUMULATION 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008650
Physical Location & Seq
Forward (+)
6365854 .. 6376538
10685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1047600.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGCCATGGCCATGGCCTTGTCATCAACACCTCGCATTCTCTCTCCTTATTGCCCAACGCACCACTCATTTCTCTCTCACACTTCTCTCTCTTCCTTACCCAGGACTGCCACCACCCTCTCCGCCTTCCCAACTTCTCCAGCGCTTCCGAAATCTCCATCGAATAGCAGAAGAATCAAGTGCGTCGATAAGGCCTCTTCGGCTTCGAGCGCCGATCCAACGTCTGGAATTGCAGTCTACAAGCCCAAGTCCTATGAAGTTCTTGTTACTGATGCTGCAAGATCGCTTGCTTATGCTCTCGAAGACGGGAAGACCCGCTTGGAGATTGATTTCCCTCCCCTGCCTAGCGACATATCTTCTTATAAGGGGTCTTCAGATGAATTCATTGATGCGAATGTACAACTTGTCTTGGCTGTTGTAAGGAAGCTCAAAGAAATACGGGAAACAAGAGCTTGCATTGTGTTTCCTGATAAGCCAGAAAAGCGCAGGGCCTCTGAGCAGTTCAAAACGGCTATCGACTCACTCGATGATATAACAATAGGTTCCCTGGATGATGTCCCCAGTGGTCCAGTCACCAAGTTCTTCCAATCTATAAGGGACACACTTGATTTTGACTTTGAAGATGAAAATGAAGGTCGCTGGGCATCCAGTCAGCCTCCCTCTCTTTATATTTTCATAAATTGTAGCACTCGTGAGCTTGCTCCAATAGAGAAGTATGTGGAAACATTTGCCATGTCAACCCCAACACTTCTATTTAATCTTGAACTAGACACATTGCGTGCCGACTTGGGCCTTTTTGGCTTTCCAACCAAAGATTTGCACTATCGCTTTCTTTCTCAATTTACACCAGTGTTCTATATCCGAATAAGAGATTACTCAAAGACAGTAGCAGTAGCGCCTTATATAGTGAATTACAGTGGAGCACTATTTCGCCAATACCCTGGGCCTTGGCAGGTAATGCTAAAACAAGCCGATGGTTCTTATGCTTGCGTAGCAGAGAGTGCAACACGCTTTACTCTGGGCGAAACAAAAGAAGAACTTTTGAGGGTCCTGGGACTGCAAGAGGAACAAGGAAGCTCGCTGCAATTTCTTCGTAGAGGCTACAGGAGTGCTACTTGGTGGGAAGAAGATGTGGAGTTGGAAGAATCTTCTGCTTGGCGTAATTGA

Protein Analysis

389

Amino Acids

43.48

Weight (kDa)

5.38

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 82 - 344 1.9e-48 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 224
AccI GTMKAC 1 cut(s) 237
AciI CCGC 2 cut(s) 123, 316
AclWI GGATC 1 cut(s) 209
AcoI YGGCCR 2 cut(s) 3, 9
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 399
AfeI AGCGCT 1 cut(s) 144
AfiI CCNNNNNNNGG 2 cut(s) 102, 224
AgsI TTSAA 3 cut(s) 505, 620, 764
AjnI CCWGG 4 cut(s) 101, 546, 940, 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AluBI AGCT 4 cut(s) 427, 452, 697, 1077
AluI AGCT 4 cut(s) 427, 452, 697, 1077
Alw21I GWGCWC 1 cut(s) 925
AlwI GGATC 1 cut(s) 209
Aor51HI AGCGCT 1 cut(s) 144
AoxI GGCC 7 cut(s) 3, 9, 15, 192, 489, 790, 944
ApeKI GCWGC 2 cut(s) 275, 1081
ApoI RAATTY 1 cut(s) 380
AspLEI GCGC 4 cut(s) 145, 212, 486, 898
AspS9I GGNCC 5 cut(s) 489, 566, 790, 944, 1048
AsuHPI GGTGA 1 cut(s) 565
AvaII GGWCC 2 cut(s) 566, 1048
BalI TGGCCA 2 cut(s) 5, 11
BauI CACGAG 1 cut(s) 690
BbsI GAAGAC 3 cut(s) 309, 317, 363
Bbv12I GWGCWC 1 cut(s) 925
BbvI GCAGC 2 cut(s) 262, 1068
BccI CCATC 2 cut(s) 166, 968
BceAI ACGGC 1 cut(s) 525
BcgI CGANNNNNNTGC 2 cut(s) 1073, 1107
BciT130I CCWGG 4 cut(s) 103, 548, 942, 1052
BfaI CTAG 2 cut(s) 345, 767
BfmI CTRYAG 1 cut(s) 1102
BfoI RGCGCY 3 cut(s) 146, 213, 899
BfuAI ACCTGC 1 cut(s) 943
BglI GCCNNNNNGGC 2 cut(s) 200, 798
BisI GCNGC 2 cut(s) 276, 1082
BlsI GCNGC 2 cut(s) 277, 1083
Bme1390I CCNGG 4 cut(s) 103, 548, 942, 1052
Bme18I GGWCC 2 cut(s) 566, 1048
BmgT120I GGNCC 5 cut(s) 489, 566, 790, 944, 1048
BmiI GGNNCC 2 cut(s) 544, 1049
BmrFI CCNGG 4 cut(s) 103, 548, 942, 1052
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 3 cut(s) 262, 379, 653
BmuI ACTGGG 1 cut(s) 555
BpiI GAAGAC 3 cut(s) 309, 317, 363
BpmI CTGGAG 1 cut(s) 123
BsaJI CCNNGG 8 cut(s) 6, 12, 101, 546, 940, 941, 947, 1051
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 224
Bse1I ACTGG 4 cut(s) 561, 569, 648, 848
Bse3DI GCAATG 1 cut(s) 773
BseBI CCWGG 4 cut(s) 103, 548, 942, 1052
BseDI CCNNGG 8 cut(s) 6, 12, 101, 546, 940, 941, 947, 1051
BseGI GGATG 2 cut(s) 556, 644
BseLI CCNNNNNNNGG 2 cut(s) 102, 224
BseMI GCAATG 1 cut(s) 773
BseMII CTCAG 1 cut(s) 486
BseNI ACTGG 4 cut(s) 561, 569, 648, 848
BseXI GCAGC 2 cut(s) 262, 1068
BseYI CCCAGC 1 cut(s) 639
BshFI GGCC 7 cut(s) 5, 11, 17, 194, 491, 792, 946
BsiHKAI GWGCWC 1 cut(s) 925
BslFI GGGAC 3 cut(s) 542, 611, 1068
BslI CCNNNNNNNGG 2 cut(s) 102, 224
BsmFI GGGAC 3 cut(s) 542, 611, 1068
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 7 cut(s) 5, 11, 17, 194, 491, 792, 946
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1407I TGTACA 1 cut(s) 397
Bsp143I GATC 2 cut(s) 214, 281
Bsp19I CCATGG 2 cut(s) 6, 12
BspACI CCGC 2 cut(s) 123, 316
BspANI GGCC 7 cut(s) 5, 11, 17, 194, 491, 792, 946
BspCNI CTCAG 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 544, 1049
BspMI ACCTGC 1 cut(s) 943
BspPI GGATC 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 773
BsrGI TGTACA 1 cut(s) 397
BsrI ACTGG 4 cut(s) 561, 569, 648, 848
BssECI CCNNGG 8 cut(s) 6, 12, 101, 546, 940, 941, 947, 1051
BssMI GATC 2 cut(s) 214, 281
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 3 cut(s) 6, 12, 947
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 4 cut(s) 103, 548, 942, 1052
Bst4CI ACNGT 2 cut(s) 886, 917
Bst6I CTCTTC 2 cut(s) 97, 202
BstAPI GCANNNNNTGC 2 cut(s) 958, 1001
BstAUI TGTACA 1 cut(s) 397
BstC8I GCNNGC 6 cut(s) 288, 454, 699, 780, 988, 1079
BstDEI CTNAG 1 cut(s) 495
BstDSI CCRYGG 2 cut(s) 6, 12
BstENI CCTNNNNNAGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 556, 644
BstH2I RGCGCY 3 cut(s) 146, 213, 899
BstHHI GCGC 4 cut(s) 145, 212, 486, 898
BstKTI GATC 2 cut(s) 217, 284
BstMBI GATC 2 cut(s) 214, 281
BstMWI GCNNNNNNNGC 7 cut(s) 200, 209, 481, 798, 958, 992, 1001
BstNI CCWGG 4 cut(s) 103, 548, 942, 1052
BstSCI CCNGG 4 cut(s) 101, 546, 940, 1050
BstSFI CTRYAG 1 cut(s) 1102
BstV1I GCAGC 2 cut(s) 262, 1068
BstV2I GAAGAC 3 cut(s) 309, 317, 363
BstXI CCANNNNNNTGG 2 cut(s) 13, 941
BsuRI GGCC 7 cut(s) 5, 11, 17, 194, 491, 792, 946
BtgI CCRYGG 2 cut(s) 6, 12
BtsCI GGATG 2 cut(s) 556, 644
BtsIMutI CAGTG 3 cut(s) 568, 855, 922
BveI ACCTGC 1 cut(s) 943
Cac8I GCNNGC 6 cut(s) 288, 454, 699, 780, 988, 1079
CfoI GCGC 4 cut(s) 145, 212, 486, 898
Cfr13I GGNCC 5 cut(s) 489, 566, 790, 944, 1048
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 398
CviAII CATG 3 cut(s) 7, 13, 733
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 1 cut(s) 495
DpnI GATC 2 cut(s) 216, 283
DpnII GATC 2 cut(s) 214, 281
EaeI YGGCCR 2 cut(s) 3, 9
Eam1104I CTCTTC 2 cut(s) 97, 202
EarI CTCTTC 2 cut(s) 97, 202
EciI GGCGGA 1 cut(s) 112
Eco130I CCWWGG 3 cut(s) 6, 12, 947
Eco147I AGGCCT 1 cut(s) 194
Eco47I GGWCC 2 cut(s) 566, 1048
Eco47III AGCGCT 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 100
EcoO109I RGGNCCY 2 cut(s) 489, 1048
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 4 cut(s) 101, 546, 940, 1050
EcoT14I CCWWGG 3 cut(s) 6, 12, 947
ErhI CCWWGG 3 cut(s) 6, 12, 947
FaeI CATG 3 cut(s) 10, 16, 736
FalI AAGNNNNNCTT 4 cut(s) 302, 334, 1023, 1055
FaqI GGGAC 3 cut(s) 542, 611, 1068
FatI CATG 3 cut(s) 6, 12, 732
FauI CCCGC 1 cut(s) 323
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 2 cut(s) 276, 1082
FokI GGATG 2 cut(s) 563, 631
Fsp4HI GCNGC 2 cut(s) 276, 1082
FspBI CTAG 2 cut(s) 345, 767
GlaI GCGC 4 cut(s) 144, 211, 485, 897
GluI GCNGC 2 cut(s) 276, 1082
GsaI CCCAGC 1 cut(s) 643
GsuI CTGGAG 1 cut(s) 123
HaeII RGCGCY 3 cut(s) 146, 213, 899
HaeIII GGCC 7 cut(s) 5, 11, 17, 194, 491, 792, 946
HgaI GACGC 1 cut(s) 172
HhaI GCGC 4 cut(s) 145, 212, 486, 898
Hin1II CATG 3 cut(s) 10, 16, 736
Hin6I GCGC 4 cut(s) 143, 210, 484, 896
HinP1I GCGC 4 cut(s) 143, 210, 484, 896
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HinfI GANTC 3 cut(s) 174, 518, 1145
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 2 cut(s) 238, 738
Hpy188I TCNGA 4 cut(s) 150, 376, 496, 863
Hpy188III TCNNGA 5 cut(s) 225, 299, 467, 692, 761
Hpy8I GTNNAC 2 cut(s) 238, 738
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 2 cut(s) 136, 626
HpyCH4III ACNGT 2 cut(s) 886, 917
HpyCH4IV ACGT 1 cut(s) 221
HpyCH4V TGCA 7 cut(s) 233, 278, 456, 820, 1004, 1060, 1084
HpyF10VI GCNNNNNNNGC 7 cut(s) 200, 209, 481, 798, 958, 992, 1001
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 1 cut(s) 221
Hsp92II CATG 3 cut(s) 10, 16, 736
HspAI GCGC 4 cut(s) 143, 210, 484, 896
Kzo9I GATC 2 cut(s) 214, 281
LmnI GCTCC 2 cut(s) 706, 920
Lsp1109I GCAGC 2 cut(s) 262, 1068
LweI GCATC 3 cut(s) 262, 379, 653
MaeI CTAG 2 cut(s) 345, 767
MaeII ACGT 1 cut(s) 221
MaeIII GTNAC 2 cut(s) 265, 571
MalI GATC 2 cut(s) 216, 283
MboI GATC 2 cut(s) 214, 281
MhlI GDGCHC 1 cut(s) 925
MlsI TGGCCA 2 cut(s) 5, 11
MluCI AATT 7 cut(s) 228, 380, 679, 839, 910, 1085, 1162
MluNI TGGCCA 2 cut(s) 5, 11
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 3 cut(s) 242, 830, 1119
Mox20I TGGCCA 2 cut(s) 5, 11
MscI TGGCCA 2 cut(s) 5, 11
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 2 cut(s) 5, 11
MspR9I CCNGG 4 cut(s) 103, 548, 942, 1052
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 4 cut(s) 103, 548, 942, 1052
MwoI GCNNNNNNNGC 7 cut(s) 200, 209, 481, 798, 958, 992, 1001
NcoI CCATGG 2 cut(s) 6, 12
NdeII GATC 2 cut(s) 214, 281
NlaIII CATG 3 cut(s) 10, 16, 736
NlaIV GGNNCC 2 cut(s) 544, 1049
NmuCI GTSAC 1 cut(s) 571
PasI CCCWGGG 1 cut(s) 941
PceI AGGCCT 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 174, 1145
PflMI CCANNNNNTGG 1 cut(s) 224
PkrI GCNGC 2 cut(s) 277, 1083
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PpuMI RGGWCCY 1 cut(s) 1048
PsiI TTATAA 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 1048
Psp6I CCWGG 4 cut(s) 101, 546, 940, 1050
PspFI CCCAGC 1 cut(s) 639
PspGI CCWGG 4 cut(s) 101, 546, 940, 1050
PspN4I GGNNCC 2 cut(s) 544, 1049
PspPI GGNCC 5 cut(s) 489, 566, 790, 944, 1048
PspPPI RGGWCCY 1 cut(s) 1048
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 11
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 2 cut(s) 276, 1082
Sau3AI GATC 2 cut(s) 214, 281
Sau96I GGNCC 5 cut(s) 489, 566, 790, 944, 1048
SchI GAGTC 1 cut(s) 512
ScrFI CCNGG 4 cut(s) 103, 548, 942, 1052
SduI GDGCHC 1 cut(s) 925
SetI ASST 9 cut(s) 34, 224, 429, 454, 544, 637, 699, 957, 1079
SfaNI GCATC 3 cut(s) 262, 379, 653
SfcI CTRYAG 1 cut(s) 1102
SinI GGWCC 2 cut(s) 566, 1048
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 7 cut(s) 228, 380, 679, 839, 910, 1085, 1162
SseBI AGGCCT 1 cut(s) 194
SsiI CCGC 2 cut(s) 123, 316
SspMI CTAG 2 cut(s) 345, 767
StuI AGGCCT 1 cut(s) 194
StyD4I CCNGG 4 cut(s) 101, 546, 940, 1050
StyI CCWWGG 3 cut(s) 6, 12, 947
TaaI ACNGT 2 cut(s) 886, 917
TaiI ACGT 1 cut(s) 224
TaqI TCGA 6 cut(s) 161, 186, 206, 300, 516, 525
TasI AATT 7 cut(s) 228, 380, 679, 839, 910, 1085, 1162
TatI WGTACW 1 cut(s) 397
TfiI GAWTC 2 cut(s) 174, 1145
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 3 cut(s) 568, 855, 922
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 2 cut(s) 275, 1081
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 6 cut(s) 270, 373, 393, 639, 645, 664
TspRI CASTG 3 cut(s) 568, 855, 922
Van91I CCANNNNNTGG 1 cut(s) 224
VpaK11BI GGWCC 2 cut(s) 566, 1048
XagI CCTNNNNNAGG 1 cut(s) 100
XapI RAATTY 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 2 cut(s) 345, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.