AT1G73180

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010315
Physical Location & Seq
Forward (+)
27517891 .. 27521710
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73180.2

Sequence Viewer

Length: 1542 bp
ATGAGTTCTTCACCGTCGTTGGAGATTTTAGTTAGAGAAGCTGAAGGCTTCACTGTGTGGAATGGTCCTCCATTTACTAATGGCCAGCCCACTCTCAAGCTTGAACGAGTTTCATGCTCTAACACGAAGTTCAGTGTTGATGGATCGAAGTTAATGGCGATGAAATCTGATGGAGTTATTAGTATCTATGATGCCACCAGCTTGACAGAAGTGCGGTCATTCACTATAGCAAATGTTACAGCTGCTGAGCTATCTCCATGTGGGACTTATCTTCAGACTTTTCAAAAACCCACTACTCCCCAAGAGAAGAATGTCTCCATTTGGAATACTGAGACAGGGGATCTAGCACACAGTCATTACCAAAAATCCATTACTAAAGCCTCATGGCCATCAATTCGCTTCTGTCCTGATGAATCTTCTGCGTGCCGGTTAGCTACAAATGAGGTCCAATTCTTTGACCCGAAAGATTTCTCGAAAGGAATAACATCCCGGATCAGAGTTCCTGGGATTGCTGCATTTGAGCTTTCTAAAACGCCAGCTTCCCATGTTGCTGTGTTTGTTCCAGAATCAAAGGGGAGTCCAGGCAGTGTCCAGATTTTTGGATGTGGGGAAGAATTGCAGAGCCAGCCAAGTGCTCGACGTAGCTTTTTCCGATGTTCCTCTGTGCAGTTTAGTTGGAACCATGGTTCCACTGGACTCCTAGTGGTTGTACAATCAGATGTTGACAAGACCAACCAAAGTTACTATGGGGAAACGAAGCTACACTACCTTACAATAGACGGCACACATGAGGGCCTTGTTCCATTACGTAAAGAAGGACCAGTTCATGATGTTCAGTGGTCCTTTTCGGGTTCAGAGTTTGCTGTTGTATATGGCTTTATGCCTGCTTGTGTGACAATCTTTGACAAGAATTGTAAACCATTGATGGAACTTGGCGAAGGTCCTTATAACACTCTTCGATGGAACCCAAAAGGGAGAGTTTTATGTGTGGCTGGATTCGGTAACTTGCCTGGTGATATGGCCTTCTGGGATGTTGTAAATAAGAAGCAGCTAGGAAGTAATAAAGCCGAGTGGTCTGTGACAAGTGAATGGTCTCCAGACGGGCGTTATTTCTTGACTGCCTCAACAGCACCAAGACGTCAAATTGACAATGGGATGAAAATCTTCAACTACGACGGAAAGCGTTATTATAAGAAGATGTTTGAGAGGCTGTATCAGGCTGAATGGAAACCAGAGTCTCCAGAGAGGTTCGGTGACATCAGTGAACTTATCAAGTCAGTTGAATCATTGAAACTCGGCGAGGGCAAATCGCAAGGACAAGGATCAGCTCAGAAGAAAACTATTGCCCCCAATCCTATTGCACAGAAACCGGCCGCATATCGACCTCCGCATGCTAAGCATGCAGCTGCTGTTCAAGCCGAGTTACTTGGAATAAGCACAACAGGAGAGATGAGCAAGAACGCTCTTAAAAACAAGAAGAAGAGGGAGAATAAGAAAGCGGCTGCGGCTGCGGCTGCTGCTTCTGGATCTAACAACGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF2A

Protein Analysis

513

Amino Acids

56.3

Weight (kDa)

9.04

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 16 - 138 1.3e-07 THOC3 beta-propeller domain
eIF2A PF08662 214 - 407 5.5e-79 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73180 AT1G73180
fragaria_vesca FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500
malus_domestica MD15G1424000.v1.1
prunus_persica Prupe.1G207000_v2.0.a1
pyrus_communis pycom15g37600
rosa_chinensis RchiOBHm_Chr2g0123631
rosa_laevigata RLG00000018761
rosa_multiflora Rmu_ssc0000091.1_g000025
rosa_roxburghii Rroxscaffold_2G00120520
rosa_rugosa Rorug02G0246500 Rorug02G0246600
rosa_samantha Rh2AG305800 Rh2BG313800 Rh2CG291900 Rh2DG329600
rosa_wichuraiana Rw2G024520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 948, 1191
AatII GACGTC 1 cut(s) 1141
AciI CCGC 6 cut(s) 214, 1374, 1388, 1499, 1505, 1511
AclWI GGATC 5 cut(s) 151, 348, 500, 1330, 1534
AcoI YGGCCR 3 cut(s) 82, 386, 1371
AcuI CTGAAG 2 cut(s) 63, 257
AcyI GRCGYC 1 cut(s) 1138
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 1 cut(s) 711
AgsI TTSAA 6 cut(s) 104, 284, 1168, 1283, 1291, 1415
AjnI CCWGG 3 cut(s) 502, 580, 1009
AloI GAACNNNNNNTCC 4 cut(s) 670, 671, 702, 703
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 4 cut(s) 319, 326, 1098, 1242
AlwI GGATC 5 cut(s) 151, 348, 500, 1330, 1534
AlwNI CAGNNNCTG 2 cut(s) 245, 1409
AoxI GGCC 5 cut(s) 82, 386, 793, 1020, 1371
ApeKI GCWGC 9 cut(s) 242, 512, 1048, 1403, 1406, 1502, 1508, 1514, 1517
Asp700I GAANNNNTTC 2 cut(s) 467, 1163
AspS9I GGNCC 6 cut(s) 65, 445, 793, 818, 840, 941
AsuC2I CCSGG 1 cut(s) 490
AsuHPI GGTGA 3 cut(s) 3, 1025, 1265
AvaII GGWCC 5 cut(s) 65, 445, 818, 840, 941
BalI TGGCCA 2 cut(s) 84, 388
BarI GAAGNNNNNNTAC 2 cut(s) 749, 781
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 9 cut(s) 229, 499, 1060, 1393, 1415, 1489, 1495, 1501, 1504
BccI CCATC 5 cut(s) 134, 164, 397, 919, 954
BceAI ACGGC 1 cut(s) 796
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 3 cut(s) 504, 582, 1011
BcnI CCSGG 1 cut(s) 490
BcoDI GTCTC 4 cut(s) 319, 326, 1098, 1242
BfaI CTAG 3 cut(s) 344, 701, 1052
BfmI CTRYAG 1 cut(s) 225
BlpI GCTNAGC 2 cut(s) 246, 1395
Bme1390I CCNGG 4 cut(s) 490, 504, 582, 1011
Bme18I GGWCC 5 cut(s) 65, 445, 818, 840, 941
BmgT120I GGNCC 6 cut(s) 65, 445, 793, 818, 840, 941
BmiI GGNNCC 3 cut(s) 680, 688, 965
BmrFI CCNGG 4 cut(s) 490, 504, 582, 1011
BmsI GCATC 1 cut(s) 181
BpmI CTGGAG 2 cut(s) 1080, 1224
Bpu1102I GCTNAGC 2 cut(s) 246, 1395
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 490
BsaAI YACGTR 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 1160
BsaHI GRCGYC 1 cut(s) 1138
BsaI GGTCTC 1 cut(s) 1098
BsaJI CCNNGG 2 cut(s) 503, 682
Bse118I RCCGGY 2 cut(s) 426, 1369
Bse1I ACTGG 2 cut(s) 697, 821
Bse8I GATNNNNATC 1 cut(s) 1160
BseBI CCWGG 3 cut(s) 504, 582, 1011
BseDI CCNNGG 2 cut(s) 503, 682
BseGI GGATG 4 cut(s) 485, 608, 1036, 1161
BseJI GATNNNNATC 1 cut(s) 1160
BseMII CTCAG 3 cut(s) 237, 321, 1343
BseNI ACTGG 2 cut(s) 697, 821
BseX3I CGGCCG 1 cut(s) 1371
BseXI GCAGC 9 cut(s) 229, 499, 1060, 1393, 1415, 1489, 1495, 1501, 1504
BsgI GTGCAG 1 cut(s) 686
Bsh1285I CGRYCG 1 cut(s) 1374
BshFI GGCC 5 cut(s) 84, 388, 795, 1022, 1373
BsiEI CGRYCG 1 cut(s) 1374
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 3 cut(s) 427, 490, 1370
BslFI GGGAC 1 cut(s) 277
BsmAI GTCTC 4 cut(s) 319, 326, 1098, 1242
BsmFI GGGAC 1 cut(s) 277
BsnI GGCC 5 cut(s) 84, 388, 795, 1022, 1373
Bso31I GGTCTC 1 cut(s) 1098
Bsp1286I GDGCHC 1 cut(s) 637
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 5 cut(s) 143, 340, 492, 1322, 1526
Bsp1720I GCTNAGC 2 cut(s) 246, 1395
Bsp19I CCATGG 1 cut(s) 682
BspACI CCGC 6 cut(s) 214, 1374, 1388, 1499, 1505, 1511
BspANI GGCC 5 cut(s) 84, 388, 795, 1022, 1373
BspCNI CTCAG 3 cut(s) 238, 322, 1342
BspHI TCATGA 1 cut(s) 826
BspLI GGNNCC 3 cut(s) 680, 688, 965
BspPI GGATC 5 cut(s) 151, 348, 500, 1330, 1534
BspTNI GGTCTC 1 cut(s) 1098
BsrFI RCCGGY 2 cut(s) 426, 1369
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 2 cut(s) 697, 821
BssAI RCCGGY 2 cut(s) 426, 1369
BssECI CCNNGG 2 cut(s) 503, 682
BssMI GATC 5 cut(s) 143, 340, 492, 1322, 1526
BssNI GRCGYC 1 cut(s) 1138
BssT1I CCWWGG 1 cut(s) 682
Bst2UI CCWGG 3 cut(s) 504, 582, 1011
Bst4CI ACNGT 3 cut(s) 15, 55, 353
Bst6I CTCTTC 2 cut(s) 960, 1475
BstACI GRCGYC 1 cut(s) 1138
BstAUI TGTACA 1 cut(s) 709
BstBAI YACGTR 1 cut(s) 809
BstC8I GCNNGC 7 cut(s) 86, 424, 537, 626, 885, 1392, 1401
BstDEI CTNAG 4 cut(s) 246, 330, 1329, 1395
BstDSI CCRYGG 1 cut(s) 682
BstF5I GGATG 4 cut(s) 485, 608, 1036, 1161
BstKTI GATC 5 cut(s) 146, 343, 495, 1325, 1529
BstMAI GTCTC 4 cut(s) 319, 326, 1098, 1242
BstMBI GATC 5 cut(s) 143, 340, 492, 1322, 1526
BstMCI CGRYCG 1 cut(s) 1374
BstNI CCWGG 3 cut(s) 504, 582, 1011
BstNSI RCATGY 2 cut(s) 1394, 1403
BstSCI CCNGG 4 cut(s) 488, 502, 580, 1009
BstSFI CTRYAG 1 cut(s) 225
BstSNI TACGTA 1 cut(s) 809
BstV1I GCAGC 9 cut(s) 229, 499, 1060, 1393, 1415, 1489, 1495, 1501, 1504
BstX2I RGATCY 2 cut(s) 340, 1526
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 2 cut(s) 340, 1526
BstZI CGGCCG 1 cut(s) 1371
BsuRI GGCC 5 cut(s) 84, 388, 795, 1022, 1373
BtgI CCRYGG 1 cut(s) 682
BtgZI GCGATG 1 cut(s) 173
BtsCI GGATG 4 cut(s) 485, 608, 1036, 1161
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 6 cut(s) 51, 139, 592, 690, 842, 1267
Cac8I GCNNGC 7 cut(s) 86, 424, 537, 626, 885, 1392, 1401
CaiI CAGNNNCTG 2 cut(s) 245, 1409
CciI TCATGA 1 cut(s) 826
Cfr10I RCCGGY 2 cut(s) 426, 1369
Cfr13I GGNCC 6 cut(s) 65, 445, 793, 818, 840, 941
Csp6I GTAC 1 cut(s) 710
CviAII CATG 9 cut(s) 114, 258, 384, 545, 683, 788, 827, 1391, 1400
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 4 cut(s) 246, 330, 1329, 1395
DpnI GATC 5 cut(s) 145, 342, 494, 1324, 1528
DpnII GATC 5 cut(s) 143, 340, 492, 1322, 1526
DraIII CACNNNGTG 1 cut(s) 57
EaeI YGGCCR 3 cut(s) 82, 386, 1371
EagI CGGCCG 1 cut(s) 1371
Eam1104I CTCTTC 2 cut(s) 960, 1475
EarI CTCTTC 2 cut(s) 960, 1475
EclXI CGGCCG 1 cut(s) 1371
Eco105I TACGTA 1 cut(s) 809
Eco130I CCWWGG 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 1098
Eco47I GGWCC 5 cut(s) 65, 445, 818, 840, 941
Eco52I CGGCCG 1 cut(s) 1371
Eco57I CTGAAG 2 cut(s) 63, 257
EcoO109I RGGNCCY 2 cut(s) 793, 941
EcoRII CCWGG 3 cut(s) 502, 580, 1009
EcoT14I CCWWGG 1 cut(s) 682
ErhI CCWWGG 1 cut(s) 682
FaeI CATG 9 cut(s) 117, 261, 387, 548, 686, 791, 830, 1394, 1403
FaqI GGGAC 1 cut(s) 277
FatI CATG 9 cut(s) 113, 257, 383, 544, 682, 787, 826, 1390, 1399
FokI GGATG 4 cut(s) 472, 615, 1043, 1168
FspBI CTAG 3 cut(s) 344, 701, 1052
GsuI CTGGAG 2 cut(s) 1080, 1224
HaeIII GGCC 5 cut(s) 84, 388, 795, 1022, 1373
HapII CCGG 3 cut(s) 427, 490, 1370
Hin1I GRCGYC 1 cut(s) 1138
Hin1II CATG 9 cut(s) 117, 261, 387, 548, 686, 791, 830, 1394, 1403
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 7 cut(s) 413, 566, 577, 696, 996, 1235, 1283
HpaII CCGG 3 cut(s) 427, 490, 1370
HphI GGTGA 3 cut(s) 3, 1025, 1265
Hpy166II GTNNAC 3 cut(s) 724, 917, 1265
Hpy188I TCNGA 7 cut(s) 169, 276, 497, 653, 718, 856, 1332
Hpy188III TCNNGA 9 cut(s) 407, 472, 563, 592, 827, 1097, 1114, 1241, 1524
Hpy8I GTNNAC 3 cut(s) 724, 917, 1265
Hpy99I CGWCG 3 cut(s) 19, 642, 1178
HpyAV CCTTC 4 cut(s) 38, 809, 932, 1033
HpyCH4III ACNGT 3 cut(s) 15, 55, 353
HpyCH4IV ACGT 3 cut(s) 640, 808, 1138
HpyCH4V TGCA 5 cut(s) 515, 619, 667, 1361, 1403
HpyF3I CTNAG 4 cut(s) 246, 330, 1329, 1395
HpySE526I ACGT 3 cut(s) 640, 808, 1138
Hsp92I GRCGYC 1 cut(s) 1138
Hsp92II CATG 9 cut(s) 117, 261, 387, 548, 686, 791, 830, 1394, 1403
Kzo9I GATC 5 cut(s) 143, 340, 492, 1322, 1526
Lsp1109I GCAGC 9 cut(s) 229, 499, 1060, 1393, 1415, 1489, 1495, 1501, 1504
LweI GCATC 1 cut(s) 181
MaeI CTAG 3 cut(s) 344, 701, 1052
MaeII ACGT 3 cut(s) 640, 808, 1138
MaeIII GTNAC 7 cut(s) 235, 740, 892, 1001, 1078, 1253, 1422
MalI GATC 5 cut(s) 145, 342, 494, 1324, 1528
MboI GATC 5 cut(s) 143, 340, 492, 1322, 1526
MflI RGATCY 2 cut(s) 340, 1526
MhlI GDGCHC 1 cut(s) 637
MlsI TGGCCA 2 cut(s) 84, 388
MluCI AATT 5 cut(s) 393, 449, 614, 910, 1143
MluNI TGGCCA 2 cut(s) 84, 388
MlyI GAGTC 3 cut(s) 586, 690, 1244
MmeI TCCRAC 1 cut(s) 656
Mox20I TGGCCA 2 cut(s) 84, 388
MroXI GAANNNNTTC 2 cut(s) 467, 1163
MscI TGGCCA 2 cut(s) 84, 388
MseI TTAA 2 cut(s) 152, 1467
Msp20I TGGCCA 2 cut(s) 84, 388
MspA1I CMGCKG 2 cut(s) 242, 1406
MspI CCGG 3 cut(s) 427, 490, 1370
MspR9I CCNGG 4 cut(s) 490, 504, 582, 1011
MvaI CCWGG 3 cut(s) 504, 582, 1011
NciI CCSGG 1 cut(s) 490
NcoI CCATGG 1 cut(s) 682
NdeII GATC 5 cut(s) 143, 340, 492, 1322, 1526
NlaIII CATG 9 cut(s) 117, 261, 387, 548, 686, 791, 830, 1394, 1403
NlaIV GGNNCC 3 cut(s) 680, 688, 965
NmeAIII GCCGAG 3 cut(s) 1093, 1275, 1444
NmuCI GTSAC 3 cut(s) 892, 1078, 1253
NspI RCATGY 2 cut(s) 1394, 1403
PaeI GCATGC 2 cut(s) 1394, 1403
PagI TCATGA 1 cut(s) 826
PdmI GAANNNNTTC 2 cut(s) 467, 1163
PfeI GAWTC 4 cut(s) 413, 566, 996, 1283
PfoI TCCNGGA 1 cut(s) 488
PleI GAGTC 3 cut(s) 585, 690, 1243
PpsI GAGTC 3 cut(s) 585, 690, 1243
Ppu21I YACGTR 1 cut(s) 809
PpuMI RGGWCCY 1 cut(s) 941
PsiI TTATAA 2 cut(s) 948, 1191
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 3 cut(s) 502, 580, 1009
PspGI CCWGG 3 cut(s) 502, 580, 1009
PspN4I GGNNCC 3 cut(s) 680, 688, 965
PspPI GGNCC 6 cut(s) 65, 445, 793, 818, 840, 941
PspPPI RGGWCCY 1 cut(s) 941
PstNI CAGNNNCTG 2 cut(s) 245, 1409
PsuI RGATCY 2 cut(s) 340, 1526
PvuII CAGCTG 2 cut(s) 242, 1406
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
SaqAI TTAA 2 cut(s) 152, 1467
Sau3AI GATC 5 cut(s) 143, 340, 492, 1322, 1526
Sau96I GGNCC 6 cut(s) 65, 445, 793, 818, 840, 941
SchI GAGTC 3 cut(s) 586, 690, 1244
ScrFI CCNGG 4 cut(s) 490, 504, 582, 1011
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 1 cut(s) 181
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 5 cut(s) 65, 445, 818, 840, 941
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
SnaBI TACGTA 1 cut(s) 809
SphI GCATGC 2 cut(s) 1394, 1403
Sse9I AATT 5 cut(s) 393, 449, 614, 910, 1143
SsiI CCGC 6 cut(s) 214, 1374, 1388, 1499, 1505, 1511
SspMI CTAG 3 cut(s) 344, 701, 1052
StyD4I CCNGG 4 cut(s) 488, 502, 580, 1009
StyI CCWWGG 1 cut(s) 682
TaaI ACNGT 3 cut(s) 15, 55, 353
TaiI ACGT 3 cut(s) 643, 811, 1141
TaqI TCGA 5 cut(s) 146, 473, 637, 958, 1381
TasI AATT 5 cut(s) 393, 449, 614, 910, 1143
TatI WGTACW 1 cut(s) 709
TauI GCSGC 4 cut(s) 1376, 1502, 1508, 1514
TfiI GAWTC 4 cut(s) 413, 566, 996, 1283
Tru1I TTAA 2 cut(s) 152, 1467
Tru9I TTAA 2 cut(s) 152, 1467
TscAI CASTG 6 cut(s) 58, 139, 592, 697, 842, 1267
TseFI GTSAC 3 cut(s) 892, 1078, 1253
TseI GCWGC 9 cut(s) 242, 512, 1048, 1403, 1406, 1502, 1508, 1514, 1517
Tsp45I GTSAC 3 cut(s) 892, 1078, 1253
TspDTI ATGAA 5 cut(s) 102, 176, 426, 815, 1172
TspGWI ACGGA 1 cut(s) 1191
TspRI CASTG 6 cut(s) 58, 139, 592, 697, 842, 1267
VpaK11BI GGWCC 5 cut(s) 65, 445, 818, 840, 941
XceI RCATGY 2 cut(s) 1394, 1403
XcmI CCANNNNNNNNNTGG 2 cut(s) 689, 743
XmnI GAANNNNTTC 2 cut(s) 467, 1163
XspI CTAG 3 cut(s) 344, 701, 1052
ZraI GACGTC 1 cut(s) 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.