Rw2G024520

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010315
Physical Location & Seq
Reverse (-)
36188491 .. 36193113
4623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024520.1

Sequence Viewer

Length: 1563 bp
ATGGCAGCCTCGGCTTCAGCTCCGCCGCCTCTCGATATCTTAGTTCGAGGGCCTGAAGGCTTCTCACTCTGGAACGGACCCCCGTTCAGTAACAACGGCCAACCCGGTATCAAGCTCGAACACAAAATCGCTTGCTCCAGTACCAAATTCAGTGACGATGGATCCAGGCTCATGGTGCTCAAACCCGATTCCGTCATCACCATCTACGATTGCCGAAGCTACGCCGAGATTAGGTCTTTCCAAGTCCCCAACCTCACCGCCGCCGCCTTGTCCCCGCGCGGCACTTTTCTTCAGACTTTCCAGAAGTCCTCAACGCCGCAGAACAAGAATGTGACCCTGTGGAACGTCGACACCGGAGAACCTGCTTACGATCAATTCCAGAAGAGTATAACCAAAACGAATTGGCCTTCGGTTAGGTTCAGCTCCGACGAGGCTGTTGCGTGTAAACTTGCGACGAATGAGATACAGTTTTTTGATGCTGCAGACTTCTCGAAAGGAGTTACAAATCGGCTGAGAGTTCCTGGAGTGGCTGCAATTGAACTCTCTAAGGCACCTGGCTCCCATGTGGCTGCATTTGTCCCGGAATCGAAGGGGATTCCTGCCAGTGTCCAGATATTTGCCACTGGAAAAGATTTACAGAGTCAACCTGTTGCTCGAAGGAGTTTCTTTCGATGTTCATCAGTGCAACTGAATTGGAATAATGGCTCAACTGGGCTTTTAGTTGTGGCGCAGTCAGATGTTGATAAAACCAACCAAAGTTACTATGGGGAATCAAAGCTAAACTATTTGACAACAGATGGAACCCATGAAGGACTTGTTCCTCTTCGCAAAGAGGGGCCTGTTCATGATGTTCAGTGGTCATACTCTGGATCAGAGTTTGCTGTAGTTTATGGCTTTATGCCTTCTAAAACAACCGTGTTTGACAAGAAGTGCAATCCTCTTCTTGAGCTTGGAACAGGCCCGTACAATACTATCCGATGGAATCCAAAGGGAAAATTTCTGTGTTTGGCAGGCTTTGGTAACTTACCAGGTGATATGGCATTTTGGGACTACATAGAGAAAAAGCAGCTTGGAACAACCAAGGCCGAATTATCTGTGACAAGCGAATGGTCTCCAGATGGACGCTATTTCATGACTGCCACGACTGCTCCGAGACTACAAGTTGACAATGGGATTAAGATTTTCCACCACAACGGTTCATTGTTCTTCAAGAAGATGTTTGACAAGCTGTTCCAGGCTGATTGGAAACCAGAATCACCTGATAGGTTCAGTGACATTTCAGAACTTGTCAAGTCCTTTGAATCAATAAAAGTTGATGACGCCAAACCTCAAGGAGAAAGGTCCAAATCTTCCCAAACCTCTGCAAAAGCCAGCCCTAACAACCCACCTGCACAAAAGCCTGCTGCATATCGCCCCCCACATGCTAAGCAAGCTGCTGTTGTTCAGGCAGAGCTGTTTGGAGGGACCACATCAGAAACAATGAGCAAGAATGCATTGCGAAACAAGAAGAAAAGGGAGAAACAAAAGGAGAAGAAGGCTACCGAGGGTGCCAATGATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF2A

Protein Analysis

520

Amino Acids

57.15

Weight (kDa)

9.18

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 220 - 413 1.4e-76 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73180 AT1G73180
fragaria_vesca FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500
malus_domestica MD15G1424000.v1.1
prunus_persica Prupe.1G207000_v2.0.a1
pyrus_communis pycom15g37600
rosa_chinensis RchiOBHm_Chr2g0123631
rosa_laevigata RLG00000018761
rosa_multiflora Rmu_ssc0000091.1_g000025
rosa_roxburghii Rroxscaffold_2G00120520
rosa_rugosa Rorug02G0246500 Rorug02G0246600
rosa_samantha Rh2AG305800 Rh2BG313800 Rh2CG291900 Rh2DG329600
rosa_wichuraiana Rw2G024520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1396
Acc36I ACCTGC 2 cut(s) 370, 1396
AccB1I GGYRCC 2 cut(s) 550, 1547
AccI GTMKAC 1 cut(s) 348
AccII CGCG 2 cut(s) 277, 279
AciI CCGC 8 cut(s) 23, 26, 258, 261, 264, 275, 279, 317
AclWI GGATC 3 cut(s) 156, 169, 877
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 146, 995
AcuI CTGAAG 2 cut(s) 75, 275
AcyI GRCGYC 1 cut(s) 1320
AfaI GTAC 2 cut(s) 142, 965
AfiI CCNNNNNNNGG 1 cut(s) 231
AgsI TTSAA 3 cut(s) 539, 1210, 1301
AjnI CCWGG 5 cut(s) 164, 520, 553, 1027, 1233
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 2 cut(s) 1116, 1147
AlwI GGATC 3 cut(s) 156, 169, 877
AoxI GGCC 6 cut(s) 50, 97, 404, 836, 958, 1083
ApeKI GCWGC 7 cut(s) 5, 479, 530, 569, 1066, 1403, 1433
ApoI RAATTY 2 cut(s) 146, 995
AspLEI GCGC 2 cut(s) 279, 730
AspS9I GGNCC 6 cut(s) 50, 77, 836, 959, 1341, 1464
AsuC2I CCSGG 2 cut(s) 105, 581
AsuHPI GGTGA 4 cut(s) 190, 247, 1043, 1248
AvaII GGWCC 3 cut(s) 77, 1341, 1464
BamHI GGATCC 1 cut(s) 161
BanI GGYRCC 2 cut(s) 550, 1547
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 7 cut(s) 17, 466, 517, 556, 1078, 1390, 1420
BccI CCATC 5 cut(s) 152, 209, 791, 972, 1112
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 4 cut(s) 419, 453, 471, 505
BciT130I CCWGG 5 cut(s) 166, 522, 555, 1029, 1235
BcnI CCSGG 2 cut(s) 105, 581
BcoDI GTCTC 2 cut(s) 1116, 1147
BfmI CTRYAG 2 cut(s) 480, 882
BfuAI ACCTGC 2 cut(s) 370, 1396
BlpI GCTNAGC 1 cut(s) 1425
Bme1390I CCNGG 7 cut(s) 105, 166, 522, 555, 581, 1029, 1235
Bme18I GGWCC 3 cut(s) 77, 1341, 1464
BmgT120I GGNCC 6 cut(s) 50, 77, 836, 959, 1341, 1464
BmiI GGNNCC 8 cut(s) 79, 163, 552, 559, 802, 837, 1465, 1549
BmrFI CCNGG 7 cut(s) 105, 166, 522, 555, 581, 1029, 1235
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 720
BpmI CTGGAG 3 cut(s) 121, 543, 1098
Bpu1102I GCTNAGC 1 cut(s) 1425
BpuEI CTTGAG 2 cut(s) 965, 1314
BpuMI CCSGG 2 cut(s) 105, 581
BsaBI GATNNNNATC 1 cut(s) 676
BsaHI GRCGYC 1 cut(s) 1320
BsaI GGTCTC 1 cut(s) 1116
BsaJI CCNNGG 3 cut(s) 9, 1080, 1542
BsaWI WCCGGW 1 cut(s) 353
BsaXI ACNNNNNCTCC 2 cut(s) 1132, 1162
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 4 cut(s) 138, 603, 628, 715
Bse3DI GCAATG 1 cut(s) 1493
Bse8I GATNNNNATC 1 cut(s) 676
BseBI CCWGG 5 cut(s) 166, 522, 555, 1029, 1235
BseDI CCNNGG 3 cut(s) 9, 1080, 1542
BseJI GATNNNNATC 1 cut(s) 676
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 1493
BseMII CTCAG 1 cut(s) 503
BseNI ACTGG 4 cut(s) 138, 603, 628, 715
BseXI GCAGC 7 cut(s) 17, 466, 517, 556, 1078, 1390, 1420
BsgI GTGCAG 1 cut(s) 1374
Bsh1236I CGCG 2 cut(s) 277, 279
BshFI GGCC 6 cut(s) 52, 99, 406, 838, 960, 1085
BshNI GGYRCC 2 cut(s) 550, 1547
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 3 cut(s) 105, 354, 581
BslFI GGGAC 5 cut(s) 230, 256, 563, 1061, 1477
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 2 cut(s) 1116, 1147
BsmFI GGGAC 5 cut(s) 230, 256, 563, 1061, 1477
BsmI GAATGC 1 cut(s) 1495
BsnI GGCC 6 cut(s) 52, 99, 406, 838, 960, 1085
Bso31I GGTCTC 1 cut(s) 1116
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 161, 370, 869
Bsp1720I GCTNAGC 1 cut(s) 1425
BspACI CCGC 8 cut(s) 23, 26, 258, 261, 264, 275, 279, 317
BspANI GGCC 6 cut(s) 52, 99, 406, 838, 960, 1085
BspCNI CTCAG 1 cut(s) 504
BspFNI CGCG 2 cut(s) 277, 279
BspHI TCATGA 2 cut(s) 844, 1131
BspLI GGNNCC 8 cut(s) 79, 163, 552, 559, 802, 837, 1465, 1549
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 2 cut(s) 370, 1396
BspPI GGATC 3 cut(s) 156, 169, 877
BspT107I GGYRCC 2 cut(s) 550, 1547
BspTNI GGTCTC 1 cut(s) 1116
BsrDI GCAATG 1 cut(s) 1493
BsrI ACTGG 4 cut(s) 138, 603, 628, 715
BssECI CCNNGG 3 cut(s) 9, 1080, 1542
BssMI GATC 3 cut(s) 161, 370, 869
BssNI GRCGYC 1 cut(s) 1320
BssT1I CCWWGG 1 cut(s) 1080
Bst2UI CCWGG 5 cut(s) 166, 522, 555, 1029, 1235
Bst4CI ACNGT 3 cut(s) 468, 916, 1196
Bst6I CTCTTC 3 cut(s) 377, 828, 945
BstACI GRCGYC 1 cut(s) 1320
BstC8I GCNNGC 5 cut(s) 133, 1012, 1372, 1401, 1431
BstDEI CTNAG 4 cut(s) 40, 512, 546, 1425
BstFNI CGCG 2 cut(s) 277, 279
BstHHI GCGC 2 cut(s) 279, 730
BstKTI GATC 3 cut(s) 164, 373, 872
BstMAI GTCTC 2 cut(s) 1116, 1147
BstMBI GATC 3 cut(s) 161, 370, 869
BstMWI GCNNNNNNNGC 4 cut(s) 11, 175, 1145, 1430
BstNI CCWGG 5 cut(s) 166, 522, 555, 1029, 1235
BstNSI RCATGY 1 cut(s) 1424
BstSCI CCNGG 7 cut(s) 103, 164, 520, 553, 579, 1027, 1233
BstSFI CTRYAG 2 cut(s) 480, 882
BstUI CGCG 2 cut(s) 277, 279
BstV1I GCAGC 7 cut(s) 17, 466, 517, 556, 1078, 1390, 1420
BstX2I RGATCY 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 172
BstYI RGATCY 1 cut(s) 161
BsuRI GGCC 6 cut(s) 52, 99, 406, 838, 960, 1085
BtsIMutI CAGTG 6 cut(s) 157, 610, 621, 687, 860, 1276
BveI ACCTGC 2 cut(s) 370, 1396
Cac8I GCNNGC 5 cut(s) 133, 1012, 1372, 1401, 1431
CciI TCATGA 2 cut(s) 844, 1131
CfoI GCGC 2 cut(s) 279, 730
Cfr13I GGNCC 6 cut(s) 50, 77, 836, 959, 1341, 1464
CseI GACGC 2 cut(s) 1131, 1328
CsiI ACCWGGT 1 cut(s) 1027
Csp6I GTAC 2 cut(s) 141, 964
CviAII CATG 6 cut(s) 172, 563, 806, 845, 1132, 1421
CviQI GTAC 2 cut(s) 141, 964
DdeI CTNAG 4 cut(s) 40, 512, 546, 1425
DpnI GATC 3 cut(s) 163, 372, 871
DpnII GATC 3 cut(s) 161, 370, 869
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 3 cut(s) 377, 828, 945
EarI CTCTTC 3 cut(s) 377, 828, 945
EciI GGCGGA 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 1080
Eco31I GGTCTC 1 cut(s) 1116
Eco32I GATATC 1 cut(s) 37
Eco47I GGWCC 3 cut(s) 77, 1341, 1464
Eco57I CTGAAG 2 cut(s) 75, 275
EcoO109I RGGNCCY 2 cut(s) 50, 836
EcoRII CCWGG 5 cut(s) 164, 520, 553, 1027, 1233
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 1080
EcoT22I ATGCAT 1 cut(s) 1495
ErhI CCWWGG 1 cut(s) 1080
FaeI CATG 6 cut(s) 175, 566, 809, 848, 1135, 1424
FaqI GGGAC 5 cut(s) 230, 256, 563, 1061, 1477
FatI CATG 6 cut(s) 171, 562, 805, 844, 1131, 1420
FauI CCCGC 1 cut(s) 282
FblI GTMKAC 1 cut(s) 348
GlaI GCGC 2 cut(s) 278, 729
GsuI CTGGAG 3 cut(s) 121, 543, 1098
HaeIII GGCC 6 cut(s) 52, 99, 406, 838, 960, 1085
HapII CCGG 3 cut(s) 105, 354, 581
HgaI GACGC 2 cut(s) 1131, 1328
HhaI GCGC 2 cut(s) 279, 730
Hin1I GRCGYC 1 cut(s) 1320
Hin1II CATG 6 cut(s) 175, 566, 809, 848, 1135, 1424
Hin6I GCGC 2 cut(s) 277, 728
HinP1I GCGC 2 cut(s) 277, 728
HincII GTYRAC 3 cut(s) 349, 644, 1165
HindII GTYRAC 3 cut(s) 349, 644, 1165
HinfI GANTC 8 cut(s) 188, 584, 595, 640, 770, 982, 1253, 1301
HpaII CCGG 3 cut(s) 105, 354, 581
HphI GGTGA 4 cut(s) 190, 247, 1043, 1248
Hpy166II GTNNAC 4 cut(s) 349, 446, 644, 1165
Hpy188I TCNGA 8 cut(s) 294, 427, 736, 874, 977, 1152, 1282, 1474
Hpy8I GTNNAC 4 cut(s) 349, 446, 644, 1165
Hpy99I CGWCG 3 cut(s) 350, 431, 457
HpyAV CCTTC 7 cut(s) 50, 417, 583, 651, 803, 912, 1528
HpyCH4III ACNGT 3 cut(s) 468, 916, 1196
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 9 cut(s) 482, 533, 572, 685, 933, 1364, 1391, 1406, 1493
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 175, 1145, 1430
HpyF3I CTNAG 4 cut(s) 40, 512, 546, 1425
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 1320
Hsp92II CATG 6 cut(s) 175, 566, 809, 848, 1135, 1424
HspAI GCGC 2 cut(s) 277, 728
Kzo9I GATC 3 cut(s) 161, 370, 869
LmnI GCTCC 5 cut(s) 25, 140, 428, 563, 1153
Lsp1109I GCAGC 7 cut(s) 17, 466, 517, 556, 1078, 1390, 1420
LweI GCATC 1 cut(s) 466
MabI ACCWGGT 1 cut(s) 1027
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 8 cut(s) 89, 152, 331, 499, 758, 1019, 1096, 1271
MalI GATC 3 cut(s) 163, 372, 871
MboI GATC 3 cut(s) 161, 370, 869
MboII GAAGA 9 cut(s) 281, 394, 815, 932, 1198, 1225, 1341, 1519, 1543
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 7 cut(s) 146, 374, 400, 534, 691, 995, 1088
MlyI GAGTC 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 450
Mph1103I ATGCAT 1 cut(s) 1495
MseI TTAA 1 cut(s) 1176
MspI CCGG 3 cut(s) 105, 354, 581
MspR9I CCNGG 7 cut(s) 105, 166, 522, 555, 581, 1029, 1235
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 1495
MvaI CCWGG 5 cut(s) 166, 522, 555, 1029, 1235
MvnI CGCG 2 cut(s) 277, 279
MwoI GCNNNNNNNGC 4 cut(s) 11, 175, 1145, 1430
NciI CCSGG 2 cut(s) 105, 581
NdeII GATC 3 cut(s) 161, 370, 869
NlaIII CATG 6 cut(s) 175, 566, 809, 848, 1135, 1424
NlaIV GGNNCC 8 cut(s) 79, 163, 552, 559, 802, 837, 1465, 1549
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 4 cut(s) 152, 331, 1096, 1271
NsiI ATGCAT 1 cut(s) 1495
NspI RCATGY 1 cut(s) 1424
PagI TCATGA 2 cut(s) 844, 1131
PaqCI CACCTGC 1 cut(s) 1396
PcsI WCGNNNNNNNCGW 1 cut(s) 1148
PctI GAATGC 1 cut(s) 1495
PfeI GAWTC 7 cut(s) 188, 584, 595, 770, 982, 1253, 1301
PfoI TCCNGGA 2 cut(s) 520, 579
PleI GAGTC 1 cut(s) 648
PpsI GAGTC 1 cut(s) 648
Psp6I CCWGG 5 cut(s) 164, 520, 553, 1027, 1233
PspGI CCWGG 5 cut(s) 164, 520, 553, 1027, 1233
PspN4I GGNNCC 8 cut(s) 79, 163, 552, 559, 802, 837, 1465, 1549
PspPI GGNCC 6 cut(s) 50, 77, 836, 959, 1341, 1464
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 161
RsaI GTAC 2 cut(s) 142, 965
RsaNI GTAC 2 cut(s) 141, 964
SalI GTCGAC 1 cut(s) 347
SaqAI TTAA 1 cut(s) 1176
Sau3AI GATC 3 cut(s) 161, 370, 869
Sau96I GGNCC 6 cut(s) 50, 77, 836, 959, 1341, 1464
SchI GAGTC 1 cut(s) 649
ScrFI CCNGG 7 cut(s) 105, 166, 522, 555, 581, 1029, 1235
SduI GDGCHC 1 cut(s) 180
SexAI ACCWGGT 1 cut(s) 1027
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 2 cut(s) 480, 882
SinI GGWCC 3 cut(s) 77, 1341, 1464
SmlI CTYRAG 2 cut(s) 944, 1329
SmoI CTYRAG 2 cut(s) 944, 1329
Sse9I AATT 7 cut(s) 146, 374, 400, 534, 691, 995, 1088
SsiI CCGC 8 cut(s) 23, 26, 258, 261, 264, 275, 279, 317
StyD4I CCNGG 7 cut(s) 103, 164, 520, 553, 579, 1027, 1233
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 3 cut(s) 468, 916, 1196
TaiI ACGT 1 cut(s) 348
TaqI TCGA 8 cut(s) 33, 46, 117, 348, 491, 587, 655, 670
TasI AATT 7 cut(s) 146, 374, 400, 534, 691, 995, 1088
TauI GCSGC 5 cut(s) 28, 263, 266, 282, 319
TfiI GAWTC 7 cut(s) 188, 584, 595, 770, 982, 1253, 1301
Tru1I TTAA 1 cut(s) 1176
Tru9I TTAA 1 cut(s) 1176
TscAI CASTG 6 cut(s) 157, 610, 628, 687, 860, 1276
TseFI GTSAC 4 cut(s) 152, 331, 1096, 1271
TseI GCWGC 7 cut(s) 5, 479, 530, 569, 1066, 1403, 1433
Tsp45I GTSAC 4 cut(s) 152, 331, 1096, 1271
TspDTI ATGAA 5 cut(s) 666, 822, 833, 1120, 1188
TspGWI ACGGA 2 cut(s) 90, 181
TspRI CASTG 6 cut(s) 157, 610, 628, 687, 860, 1276
VpaK11BI GGWCC 3 cut(s) 77, 1341, 1464
XapI RAATTY 2 cut(s) 146, 995
XceI RCATGY 1 cut(s) 1424
XcmI CCANNNNNNNNNTGG 1 cut(s) 761
XmiI GTMKAC 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.