Rroxscaffold_2G00120520

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010315
Physical Location & Seq
Forward (+)
52785813 .. 52803167
17355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00120520.1

Sequence Viewer

Length: 1563 bp
ATGGACAAGATCGTGAACATTGACGCGGCGGGATTGGAGGTGAGACGAGGATGTCCAAATAACAACGGCCAACCCGGTATCAAGCTCGAACACAAAATCGCTTGCTCCAGTACCAAATTCAGTGACGATGGATCCAGGCTCATGGTGCTCAAACCCGATTCCGTCATCACCATCTTCGATTGCCGAAGCTACACCGAGATTAGGTCTTTCCAAGTCCCCAATCTCACCGCCGCCGCCTTGTCCCCGCGTGGCACTTTTCTTCAGACTTTCCACAAGTCCTCAACGCCGCAGAACAAGAATGTGACCCTGTGGAACGTCGACACCGGAGAACCTGCTTACGATCAATTCCAGAAGAGTATAACCAAAACGAATTGGCCCTCGGTTAGGTTCAGCTCCGACGAGGCTGTTGCGTGTAAACTTGCGACGAATGAGATACAATTTTTTGATGCTGCAGACTTCTCGAAAGGAGTTACGAATCGGCTGAGAGTTCCTGGAGTTGCTGCAATTGAACTCTCTAAGGCACCTGGCTCCCATGTCGCTGCATTTGTCCCGGAATCGAAGGGGATTCCCGCCAGTGTCCAGATATTTGCTACTGGAAAAGATTTACAGAGTCAACCTGTTGCTCGAAGGAGTTTCTTTCGATGTTCATCAGTGCAACTGAATTGGAATAATGGCTCAACTGGGCTTTTAGTTGTGGCGCAGTCAGATGTTGATAAAACCAACCAAAGTTACTATGGGGAATCAAAGCTAAACTACTTGACAACAGATGGAACCCATGAAGGACTTGTACCTCTTCGTAAAGAGGGGCCTGTTCATGATGTTCAGTGGTCATACTCTGGATCAGAGTTTGCTGTAGTTTATGGCTTTATGCCTTCTAAAACAACCGTGTTTGACAAGAAGTGCAATCCTCTTCTTGAGCTTGGAACAGGCCCGTACAATACTATCCGATGGAATCCAAAGGGAAAATTTCTGTGTTTGGCAGGCTTTGGTAACTTACCAGGTGATATGGCATTTTGGGACTACATAGAGAAAAAGCAGCTTGGAACAACCAAGGCCGAATTATCTGTGACAAGCGAATGGTCTCCAGATGGACGCTATTTCATGACTGCCACGACTGCTCCAAGACTACAAGTTGACAATGGGATTAAGATTTTCCACCACAACGGTTCATTGTTCTTCAAGAAGATGTTTGACAAGCTGTTCCAGGCTGATTGGAAACCAGAATCACCTGATAGGTTCAGTGACATTTCAGAACTTGTCAAGTCCTTTGAATCAATAAAAAGGTACGAGCGCGATGTCGCCGGAAGCGGCTCCACGATCTCTGCGCTGCTGTCAATTTCGGAGCTCATCAATGCGGAGGACAGAACTTGGAAGCAGGACAAAAAGAGCACAACACGCCGGCCACCATGGGCCGCGGCGGCGTCAACATGGCCGGCGGTACTCTTGAGGGGTAAGGCGCTATGCGGAGAGCTTCCGAACAATATTTCGCGCAACCCAAAGCCACGTTATAAGAAAGGTGGGATCAAACGTGCAGCGGTGCTGCAGGGCAGCAGGGATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF2A

Protein Analysis

520

Amino Acids

57.53

Weight (kDa)

9.3

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 210 - 403 1.4e-76 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73180 AT1G73180
fragaria_vesca FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500
malus_domestica MD15G1424000.v1.1
prunus_persica Prupe.1G207000_v2.0.a1
pyrus_communis pycom15g37600
rosa_chinensis RchiOBHm_Chr2g0123631
rosa_laevigata RLG00000018761
rosa_multiflora Rmu_ssc0000091.1_g000025
rosa_roxburghii Rroxscaffold_2G00120520
rosa_rugosa Rorug02G0246500 Rorug02G0246600
rosa_samantha Rh2AG305800 Rh2BG313800 Rh2CG291900 Rh2DG329600
rosa_wichuraiana Rw2G024520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1509
AasI GACNNNNNNGTC 1 cut(s) 51
Acc36I ACCTGC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 520
AccI GTMKAC 1 cut(s) 318
AccII CGCG 5 cut(s) 26, 247, 1293, 1415, 1489
AclWI GGATC 4 cut(s) 126, 139, 847, 1529
AcoI YGGCCR 3 cut(s) 67, 1400, 1430
AcsI RAATTY 2 cut(s) 116, 965
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 1 cut(s) 1421
AfaI GTAC 5 cut(s) 112, 789, 935, 1286, 1440
AfiI CCNNNNNNNGG 2 cut(s) 201, 384
AgsI TTSAA 3 cut(s) 509, 1180, 1271
AjnI CCWGG 5 cut(s) 134, 490, 523, 997, 1203
AluBI AGCT 9 cut(s) 85, 189, 393, 748, 919, 1039, 1198, 1345, 1471
AluI AGCT 9 cut(s) 85, 189, 393, 748, 919, 1039, 1198, 1345, 1471
Alw21I GWGCWC 3 cut(s) 150, 1347, 1391
Alw26I GTCTC 2 cut(s) 37, 1086
AlwI GGATC 4 cut(s) 126, 139, 847, 1529
AoxI GGCC 8 cut(s) 67, 374, 806, 928, 1053, 1400, 1410, 1430
ApeKI GCWGC 8 cut(s) 449, 500, 539, 1036, 1327, 1532, 1540, 1548
ApoI RAATTY 2 cut(s) 116, 965
AspLEI GCGC 5 cut(s) 700, 1293, 1327, 1459, 1491
AspS9I GGNCC 4 cut(s) 375, 806, 929, 1410
AsuC2I CCSGG 2 cut(s) 75, 551
AsuHPI GGTGA 5 cut(s) 52, 160, 217, 1013, 1218
BamHI GGATCC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 520
BanII GRGCYC 1 cut(s) 1347
BarI GAAGNNNNNNTAC 2 cut(s) 771, 803
Bbv12I GWGCWC 3 cut(s) 150, 1347, 1391
BbvI GCAGC 7 cut(s) 436, 487, 526, 1048, 1314, 1527, 1544
BccI CCATC 5 cut(s) 122, 179, 761, 942, 1082
BceAI ACGGC 1 cut(s) 82
BcgI CGANNNNNNTGC 4 cut(s) 389, 423, 441, 475
BciT130I CCWGG 5 cut(s) 136, 492, 525, 999, 1205
BcnI CCSGG 2 cut(s) 75, 551
BcoDI GTCTC 2 cut(s) 37, 1086
BfmI CTRYAG 3 cut(s) 450, 852, 1541
BfoI RGCGCY 1 cut(s) 1460
BfuAI ACCTGC 1 cut(s) 340
BglI GCCNNNNNGGC 1 cut(s) 1418
Bme1390I CCNGG 7 cut(s) 75, 136, 492, 525, 551, 999, 1205
BmgT120I GGNCC 4 cut(s) 375, 806, 929, 1410
BmiI GGNNCC 6 cut(s) 133, 522, 529, 772, 807, 1312
BmrFI CCNGG 7 cut(s) 75, 136, 492, 525, 551, 999, 1205
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 2 cut(s) 436, 1546
BmuI ACTGGG 1 cut(s) 690
BpmI CTGGAG 3 cut(s) 91, 513, 1068
BpuEI CTTGAG 2 cut(s) 935, 1465
BpuMI CCSGG 2 cut(s) 75, 551
BsaBI GATNNNNATC 1 cut(s) 646
BsaHI GRCGYC 1 cut(s) 1421
BsaI GGTCTC 1 cut(s) 1086
BsaJI CCNNGG 4 cut(s) 378, 1050, 1406, 1413
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 1102, 1132
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 384
Bse118I RCCGGY 2 cut(s) 1398, 1432
Bse1I ACTGG 4 cut(s) 108, 573, 598, 685
Bse8I GATNNNNATC 1 cut(s) 646
BseBI CCWGG 5 cut(s) 136, 492, 525, 999, 1205
BseDI CCNNGG 4 cut(s) 378, 1050, 1406, 1413
BseGI GGATG 2 cut(s) 56, 1561
BseJI GATNNNNATC 1 cut(s) 646
BseLI CCNNNNNNNGG 2 cut(s) 201, 384
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 4 cut(s) 108, 573, 598, 685
BseXI GCAGC 7 cut(s) 436, 487, 526, 1048, 1314, 1527, 1544
BsgI GTGCAG 1 cut(s) 1551
Bsh1236I CGCG 5 cut(s) 26, 247, 1293, 1415, 1489
BshFI GGCC 8 cut(s) 69, 376, 808, 930, 1055, 1402, 1412, 1432
BshNI GGYRCC 1 cut(s) 520
BsiHKAI GWGCWC 3 cut(s) 150, 1347, 1391
BsiSI CCGG 6 cut(s) 75, 324, 551, 1302, 1399, 1433
BslFI GGGAC 4 cut(s) 200, 226, 533, 1031
BslI CCNNNNNNNGG 2 cut(s) 201, 384
BsmAI GTCTC 2 cut(s) 37, 1086
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 4 cut(s) 200, 226, 533, 1031
BsnI GGCC 8 cut(s) 69, 376, 808, 930, 1055, 1402, 1412, 1432
Bso31I GGTCTC 1 cut(s) 1086
Bsp1286I GDGCHC 3 cut(s) 150, 1347, 1391
Bsp143I GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
Bsp19I CCATGG 1 cut(s) 1406
BspANI GGCC 8 cut(s) 69, 376, 808, 930, 1055, 1402, 1412, 1432
BspCNI CTCAG 1 cut(s) 474
BspFNI CGCG 5 cut(s) 26, 247, 1293, 1415, 1489
BspHI TCATGA 2 cut(s) 814, 1101
BspLI GGNNCC 6 cut(s) 133, 522, 529, 772, 807, 1312
BspMAI CTGCAG 2 cut(s) 454, 1545
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 4 cut(s) 126, 139, 847, 1529
BspT107I GGYRCC 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 1086
BsrFI RCCGGY 2 cut(s) 1398, 1432
BsrI ACTGG 4 cut(s) 108, 573, 598, 685
BssAI RCCGGY 2 cut(s) 1398, 1432
BssECI CCNNGG 4 cut(s) 378, 1050, 1406, 1413
BssMI GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
BssNI GRCGYC 1 cut(s) 1421
BssT1I CCWWGG 2 cut(s) 1050, 1406
Bst2UI CCWGG 5 cut(s) 136, 492, 525, 999, 1205
Bst4CI ACNGT 2 cut(s) 886, 1166
Bst6I CTCTTC 3 cut(s) 347, 798, 915
BstACI GRCGYC 1 cut(s) 1421
BstC8I GCNNGC 4 cut(s) 103, 982, 1400, 1434
BstDEI CTNAG 2 cut(s) 482, 516
BstDSI CCRYGG 2 cut(s) 1406, 1413
BstENI CCTNNNNNAGG 1 cut(s) 382
BstF5I GGATG 2 cut(s) 56, 1561
BstFNI CGCG 5 cut(s) 26, 247, 1293, 1415, 1489
BstH2I RGCGCY 1 cut(s) 1460
BstHHI GCGC 5 cut(s) 700, 1293, 1327, 1459, 1491
BstKTI GATC 6 cut(s) 12, 134, 343, 842, 1320, 1524
BstMAI GTCTC 2 cut(s) 37, 1086
BstMBI GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
BstMWI GCNNNNNNNGC 4 cut(s) 145, 1115, 1395, 1418
BstNI CCWGG 5 cut(s) 136, 492, 525, 999, 1205
BstSCI CCNGG 7 cut(s) 73, 134, 490, 523, 549, 997, 1203
BstSFI CTRYAG 3 cut(s) 450, 852, 1541
BstUI CGCG 5 cut(s) 26, 247, 1293, 1415, 1489
BstV1I GCAGC 7 cut(s) 436, 487, 526, 1048, 1314, 1527, 1544
BstX2I RGATCY 1 cut(s) 131
BstXI CCANNNNNNTGG 1 cut(s) 142
BstYI RGATCY 1 cut(s) 131
BsuRI GGCC 8 cut(s) 69, 376, 808, 930, 1055, 1402, 1412, 1432
BtgI CCRYGG 2 cut(s) 1406, 1413
BtgZI GCGATG 1 cut(s) 1308
BtsCI GGATG 2 cut(s) 56, 1561
BtsIMutI CAGTG 5 cut(s) 127, 580, 657, 830, 1246
BveI ACCTGC 1 cut(s) 340
Cac8I GCNNGC 4 cut(s) 103, 982, 1400, 1434
CciI TCATGA 2 cut(s) 814, 1101
CfoI GCGC 5 cut(s) 700, 1293, 1327, 1459, 1491
Cfr10I RCCGGY 2 cut(s) 1398, 1432
Cfr13I GGNCC 4 cut(s) 375, 806, 929, 1410
Cfr42I CCGCGG 1 cut(s) 1416
CseI GACGC 3 cut(s) 32, 1101, 1410
CsiI ACCWGGT 1 cut(s) 997
Csp6I GTAC 5 cut(s) 111, 788, 934, 1285, 1439
CviAII CATG 7 cut(s) 142, 533, 776, 815, 1102, 1407, 1428
CviQI GTAC 5 cut(s) 111, 788, 934, 1285, 1439
DdeI CTNAG 2 cut(s) 482, 516
DpnI GATC 6 cut(s) 11, 133, 342, 841, 1319, 1523
DpnII GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
DrdI GACNNNNNNGTC 1 cut(s) 51
DseDI GACNNNNNNGTC 1 cut(s) 51
EaeI YGGCCR 3 cut(s) 67, 1400, 1430
Eam1104I CTCTTC 3 cut(s) 347, 798, 915
EarI CTCTTC 3 cut(s) 347, 798, 915
Ecl136II GAGCTC 1 cut(s) 1345
Eco130I CCWWGG 2 cut(s) 1050, 1406
Eco24I GRGCYC 1 cut(s) 1347
Eco31I GGTCTC 1 cut(s) 1086
Eco53kI GAGCTC 1 cut(s) 1345
Eco57I CTGAAG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 1345
EcoNI CCTNNNNNAGG 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 806
EcoRII CCWGG 5 cut(s) 134, 490, 523, 997, 1203
EcoT14I CCWWGG 2 cut(s) 1050, 1406
EcoT38I GRGCYC 1 cut(s) 1347
ErhI CCWWGG 2 cut(s) 1050, 1406
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 7 cut(s) 145, 536, 779, 818, 1105, 1410, 1431
FaqI GGGAC 4 cut(s) 200, 226, 533, 1031
FatI CATG 7 cut(s) 141, 532, 775, 814, 1101, 1406, 1427
FauI CCCGC 3 cut(s) 22, 252, 577
FblI GTMKAC 1 cut(s) 318
FokI GGATG 1 cut(s) 63
FriOI GRGCYC 1 cut(s) 1347
GlaI GCGC 5 cut(s) 699, 1292, 1326, 1458, 1490
GsuI CTGGAG 3 cut(s) 91, 513, 1068
HaeII RGCGCY 1 cut(s) 1460
HaeIII GGCC 8 cut(s) 69, 376, 808, 930, 1055, 1402, 1412, 1432
HapII CCGG 6 cut(s) 75, 324, 551, 1302, 1399, 1433
HgaI GACGC 3 cut(s) 32, 1101, 1410
HhaI GCGC 5 cut(s) 700, 1293, 1327, 1459, 1491
Hin1I GRCGYC 1 cut(s) 1421
Hin1II CATG 7 cut(s) 145, 536, 779, 818, 1105, 1410, 1431
Hin6I GCGC 5 cut(s) 698, 1291, 1325, 1457, 1489
HinP1I GCGC 5 cut(s) 698, 1291, 1325, 1457, 1489
HincII GTYRAC 4 cut(s) 319, 614, 1135, 1425
HindII GTYRAC 4 cut(s) 319, 614, 1135, 1425
HinfI GANTC 9 cut(s) 158, 475, 554, 565, 610, 740, 952, 1223, 1271
HpaII CCGG 6 cut(s) 75, 324, 551, 1302, 1399, 1433
HphI GGTGA 5 cut(s) 52, 160, 217, 1013, 1218
Hpy166II GTNNAC 6 cut(s) 16, 319, 416, 614, 1135, 1425
Hpy188I TCNGA 8 cut(s) 264, 397, 706, 844, 947, 1252, 1342, 1476
Hpy8I GTNNAC 6 cut(s) 16, 319, 416, 614, 1135, 1425
Hpy99I CGWCG 3 cut(s) 320, 401, 427
HpyAV CCTTC 4 cut(s) 553, 621, 773, 882
HpyCH4III ACNGT 2 cut(s) 886, 1166
HpyCH4IV ACGT 3 cut(s) 315, 1504, 1528
HpyCH4V TGCA 7 cut(s) 452, 503, 542, 655, 903, 1532, 1543
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 1115, 1395, 1418
HpyF3I CTNAG 2 cut(s) 482, 516
HpySE526I ACGT 3 cut(s) 315, 1504, 1528
Hsp92I GRCGYC 1 cut(s) 1421
Hsp92II CATG 7 cut(s) 145, 536, 779, 818, 1105, 1410, 1431
HspAI GCGC 5 cut(s) 698, 1291, 1325, 1457, 1489
KroI GCCGGC 2 cut(s) 1398, 1432
KroNI GCCGGC 2 cut(s) 1400, 1434
KspI CCGCGG 1 cut(s) 1416
Kzo9I GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
LmnI GCTCC 6 cut(s) 110, 398, 533, 1123, 1316, 1342
Lsp1109I GCAGC 7 cut(s) 436, 487, 526, 1048, 1314, 1527, 1544
LweI GCATC 2 cut(s) 436, 1546
MabI ACCWGGT 1 cut(s) 997
MaeII ACGT 3 cut(s) 315, 1504, 1528
MaeIII GTNAC 7 cut(s) 122, 301, 469, 728, 989, 1066, 1241
MalI GATC 6 cut(s) 11, 133, 342, 841, 1319, 1523
MboI GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
MboII GAAGA 7 cut(s) 166, 251, 364, 785, 902, 1168, 1195
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 3 cut(s) 150, 1347, 1391
MluCI AATT 9 cut(s) 116, 344, 370, 437, 504, 661, 965, 1058, 1335
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 1 cut(s) 420
MroNI GCCGGC 2 cut(s) 1398, 1432
MseI TTAA 1 cut(s) 1146
MspA1I CMGCKG 2 cut(s) 1415, 1535
MspI CCGG 6 cut(s) 75, 324, 551, 1302, 1399, 1433
MspR9I CCNGG 7 cut(s) 75, 136, 492, 525, 551, 999, 1205
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 5 cut(s) 136, 492, 525, 999, 1205
MvnI CGCG 5 cut(s) 26, 247, 1293, 1415, 1489
MwoI GCNNNNNNNGC 4 cut(s) 145, 1115, 1395, 1418
NaeI GCCGGC 2 cut(s) 1400, 1434
NciI CCSGG 2 cut(s) 75, 551
NcoI CCATGG 1 cut(s) 1406
NdeII GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
NgoMIV GCCGGC 2 cut(s) 1398, 1432
NlaIII CATG 7 cut(s) 145, 536, 779, 818, 1105, 1410, 1431
NlaIV GGNNCC 6 cut(s) 133, 522, 529, 772, 807, 1312
NmuCI GTSAC 4 cut(s) 122, 301, 1066, 1241
PagI TCATGA 2 cut(s) 814, 1101
PdiI GCCGGC 2 cut(s) 1400, 1434
PfeI GAWTC 8 cut(s) 158, 475, 554, 565, 740, 952, 1223, 1271
PfoI TCCNGGA 2 cut(s) 490, 549
PleI GAGTC 1 cut(s) 618
PpsI GAGTC 1 cut(s) 618
PsiI TTATAA 1 cut(s) 1509
Psp124BI GAGCTC 1 cut(s) 1347
Psp6I CCWGG 5 cut(s) 134, 490, 523, 997, 1203
PspGI CCWGG 5 cut(s) 134, 490, 523, 997, 1203
PspN4I GGNNCC 6 cut(s) 133, 522, 529, 772, 807, 1312
PspPI GGNCC 4 cut(s) 375, 806, 929, 1410
PstI CTGCAG 2 cut(s) 454, 1545
PsuI RGATCY 1 cut(s) 131
RsaI GTAC 5 cut(s) 112, 789, 935, 1286, 1440
RsaNI GTAC 5 cut(s) 111, 788, 934, 1285, 1439
SacI GAGCTC 1 cut(s) 1347
SacII CCGCGG 1 cut(s) 1416
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 1 cut(s) 1146
Sau3AI GATC 6 cut(s) 9, 131, 340, 839, 1317, 1521
Sau96I GGNCC 4 cut(s) 375, 806, 929, 1410
SchI GAGTC 1 cut(s) 619
ScrFI CCNGG 7 cut(s) 75, 136, 492, 525, 551, 999, 1205
SduI GDGCHC 3 cut(s) 150, 1347, 1391
SexAI ACCWGGT 1 cut(s) 997
SfaNI GCATC 2 cut(s) 436, 1546
SfcI CTRYAG 3 cut(s) 450, 852, 1541
Sfr303I CCGCGG 1 cut(s) 1416
SgrBI CCGCGG 1 cut(s) 1416
SmlI CTYRAG 2 cut(s) 914, 1444
SmoI CTYRAG 2 cut(s) 914, 1444
Sse9I AATT 9 cut(s) 116, 344, 370, 437, 504, 661, 965, 1058, 1335
SspI AATATT 1 cut(s) 1483
SstI GAGCTC 1 cut(s) 1347
StyD4I CCNGG 7 cut(s) 73, 134, 490, 523, 549, 997, 1203
StyI CCWWGG 2 cut(s) 1050, 1406
TaaI ACNGT 2 cut(s) 886, 1166
TaiI ACGT 3 cut(s) 318, 1507, 1531
TaqI TCGA 7 cut(s) 87, 177, 318, 461, 557, 625, 640
TasI AATT 9 cut(s) 116, 344, 370, 437, 504, 661, 965, 1058, 1335
TauI GCSGC 8 cut(s) 29, 233, 236, 289, 1311, 1415, 1418, 1421
TfiI GAWTC 8 cut(s) 158, 475, 554, 565, 740, 952, 1223, 1271
Tru1I TTAA 1 cut(s) 1146
Tru9I TTAA 1 cut(s) 1146
TscAI CASTG 5 cut(s) 127, 580, 657, 830, 1246
TseFI GTSAC 4 cut(s) 122, 301, 1066, 1241
TseI GCWGC 8 cut(s) 449, 500, 539, 1036, 1327, 1532, 1540, 1548
Tsp45I GTSAC 4 cut(s) 122, 301, 1066, 1241
TspDTI ATGAA 5 cut(s) 636, 792, 803, 1090, 1158
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 5 cut(s) 127, 580, 657, 830, 1246
XagI CCTNNNNNAGG 1 cut(s) 382
XapI RAATTY 2 cut(s) 116, 965
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XmiI GTMKAC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.