Rh2BG313800

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010315
Physical Location & Seq
Reverse (-)
36931003 .. 36936216
5214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG313800.1

Sequence Viewer

Length: 1560 bp
ATGGCAGCCTCGGCTTCAGCTCCGCCTCTCGATATCTTAGTTCGAGGGCCTGAAGGCTTCTCACTCTGGAACGGACCCCCGTTCAGTAACAACGGCCAACCCGGTATCAAGCTCGAACACAAAATCGCTTGCTCCAGTACCAAATTCAGTGACGATGGATCGAGGCTCATGGTGCTCAAACCCGATTCCGTCATCACCATCTACGATTGCCGAAGCTACGCCGAGATTAGGTCTTTCCAAGTCCCCAACCTCACCGCCGCCGCCTTGTCCCCGCGCGGCACTTTTCTTCAGACTTTCCAGAAGTCCTCAACGCCGCAGAACAAGAATGTGACCCTGTGGAACGTCGACACCGGAGAACCTGCTTACGATCAATTCCAGAAGAGTATAACCAAAACGAATTGGCCCTCGGTTAGGTTCAGCTCCGACGAGGCTGTTGCGTGTAAACTTGCGACGAATGAGATACAGTTTTTTGATGCTGCAGACTTCTCGAAAGGAGTTACGAATCGGCTGAGAGTTCCTGGAGTGGCTGCAATTGAACTCTCTAAGGCACCTGGCTCCCATGTGGCTGCATTTGTCCCGGAATCGAAGGGGATTCCTGCCAGTGTCCAGATATTTGCCACTGGAAAAGATTTACAGAGTCAACCTGTTGCTCGAAGGAGTTTCTTTCGATGTTCATCAGTGCAACTGAATTGGAATAATGGCTCAACTGGGCTTTTAGTTGTGGCGCAGTCAGATGTTGATAAAACCAACCAAAGTTACTATGGGGAATCAAAGCTAAACTACTTGACAACAGATGGAACCCATGAAGGACTTGTTCCTCTTCGTAAAGAGGGGCCTGTTCATGATGTTCAGTGGTCATACTCTGGATCAGAGTTTGCTGTAGTTTATGGCTTTATGCCTTCTAAAACAACCGTGTTTGACAAGAAGTGCAATCCTCTTCTTGAGCTTGGAACAGGCCCGTACAATACTATCCGATGGAATCCAAAGGGAAAATTTCTGTGTTTGGCAGGCTTTGGTAACTTACCAGGTGATATGGCATTTTGGGACTACATAGAGAAAAAGCAGCTTGGAACAACCAAGGCCGAATTATCTGTGACAAGCGAATGGTCTCCAGATGGACGCTATTTCATGACTGCCACGACTGCTCCGAGACTACAAGTTGACAATGGGATTAAGATTTTCCACCACAACGGTTCATTGTTCTTCAAGAAGATGTTTGACAAGCTGTTCCAGGCTGATTGGAAACCAGAATCACCTGATAGGTTCAGTGACATTTCAGAACTTGTCAAGTCCTTTGAATCAATAAAAGTTGATGACGCCAAACCTCAAGGAGAAAGGTCCAAATCTTCCCAAACCTCTGCAAAAGCCAGCCCTAACAACCCAACTGCACAAAAGCCTGCTGCATATCGCCCCCCACATGCTAAGCAAGCTGCTGTCGTTCAGGCAGAGCTGTTTGGAGGGACCACATCAGAAACAATGAGCAAGAATGCATTGCGAAACAAGAAGAAAAGGGAGAAACAAAAGGAGAAGAAGGCTACCGAGGGTGCCAATGATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF2A

Protein Analysis

519

Amino Acids

57.05

Weight (kDa)

9.18

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 219 - 412 1.4e-76 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73180 AT1G73180
fragaria_vesca FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500
malus_domestica MD15G1424000.v1.1
prunus_persica Prupe.1G207000_v2.0.a1
pyrus_communis pycom15g37600
rosa_chinensis RchiOBHm_Chr2g0123631
rosa_laevigata RLG00000018761
rosa_multiflora Rmu_ssc0000091.1_g000025
rosa_roxburghii Rroxscaffold_2G00120520
rosa_rugosa Rorug02G0246500 Rorug02G0246600
rosa_samantha Rh2AG305800 Rh2BG313800 Rh2CG291900 Rh2DG329600
rosa_wichuraiana Rw2G024520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 367
AccB1I GGYRCC 2 cut(s) 547, 1544
AccI GTMKAC 1 cut(s) 345
AccII CGCG 2 cut(s) 274, 276
AciI CCGC 7 cut(s) 23, 255, 258, 261, 272, 276, 314
AclWI GGATC 2 cut(s) 166, 874
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 2 cut(s) 143, 992
AcuI CTGAAG 2 cut(s) 72, 272
AcyI GRCGYC 1 cut(s) 1317
AfaI GTAC 2 cut(s) 139, 962
AfiI CCNNNNNNNGG 2 cut(s) 228, 411
AgsI TTSAA 3 cut(s) 536, 1207, 1298
AjnI CCWGG 4 cut(s) 517, 550, 1024, 1230
Alw21I GWGCWC 1 cut(s) 177
Alw26I GTCTC 2 cut(s) 1113, 1144
AlwI GGATC 2 cut(s) 166, 874
AoxI GGCC 6 cut(s) 47, 94, 401, 833, 955, 1080
ApeKI GCWGC 7 cut(s) 5, 476, 527, 566, 1063, 1400, 1430
ApoI RAATTY 2 cut(s) 143, 992
AspLEI GCGC 2 cut(s) 276, 727
AspS9I GGNCC 7 cut(s) 47, 74, 402, 833, 956, 1338, 1461
AsuC2I CCSGG 2 cut(s) 102, 578
AsuHPI GGTGA 4 cut(s) 187, 244, 1040, 1245
AvaII GGWCC 3 cut(s) 74, 1338, 1461
BanI GGYRCC 2 cut(s) 547, 1544
Bbv12I GWGCWC 1 cut(s) 177
BbvI GCAGC 7 cut(s) 17, 463, 514, 553, 1075, 1387, 1417
BccI CCATC 5 cut(s) 149, 206, 788, 969, 1109
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 4 cut(s) 416, 450, 468, 502
BciT130I CCWGG 4 cut(s) 519, 552, 1026, 1232
BcnI CCSGG 2 cut(s) 102, 578
BcoDI GTCTC 2 cut(s) 1113, 1144
BfmI CTRYAG 2 cut(s) 477, 879
BfuAI ACCTGC 1 cut(s) 367
BlpI GCTNAGC 1 cut(s) 1422
Bme1390I CCNGG 6 cut(s) 102, 519, 552, 578, 1026, 1232
Bme18I GGWCC 3 cut(s) 74, 1338, 1461
BmgT120I GGNCC 7 cut(s) 47, 74, 402, 833, 956, 1338, 1461
BmiI GGNNCC 7 cut(s) 76, 549, 556, 799, 834, 1462, 1546
BmrFI CCNGG 6 cut(s) 102, 519, 552, 578, 1026, 1232
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 717
BpmI CTGGAG 3 cut(s) 118, 540, 1095
Bpu1102I GCTNAGC 1 cut(s) 1422
BpuEI CTTGAG 2 cut(s) 962, 1311
BpuMI CCSGG 2 cut(s) 102, 578
BsaBI GATNNNNATC 1 cut(s) 673
BsaHI GRCGYC 1 cut(s) 1317
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 4 cut(s) 9, 405, 1077, 1539
BsaWI WCCGGW 1 cut(s) 350
BsaXI ACNNNNNCTCC 2 cut(s) 1129, 1159
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 411
Bse1I ACTGG 4 cut(s) 135, 600, 625, 712
Bse3DI GCAATG 1 cut(s) 1490
Bse8I GATNNNNATC 1 cut(s) 673
BseBI CCWGG 4 cut(s) 519, 552, 1026, 1232
BseDI CCNNGG 4 cut(s) 9, 405, 1077, 1539
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 2 cut(s) 228, 411
BseMI GCAATG 1 cut(s) 1490
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 4 cut(s) 135, 600, 625, 712
BseXI GCAGC 7 cut(s) 17, 463, 514, 553, 1075, 1387, 1417
BsgI GTGCAG 1 cut(s) 1371
Bsh1236I CGCG 2 cut(s) 274, 276
BshFI GGCC 6 cut(s) 49, 96, 403, 835, 957, 1082
BshNI GGYRCC 2 cut(s) 547, 1544
BsiHKAI GWGCWC 1 cut(s) 177
BsiSI CCGG 3 cut(s) 102, 351, 578
BslFI GGGAC 5 cut(s) 227, 253, 560, 1058, 1474
BslI CCNNNNNNNGG 2 cut(s) 228, 411
BsmAI GTCTC 2 cut(s) 1113, 1144
BsmFI GGGAC 5 cut(s) 227, 253, 560, 1058, 1474
BsmI GAATGC 1 cut(s) 1492
BsnI GGCC 6 cut(s) 49, 96, 403, 835, 957, 1082
Bso31I GGTCTC 1 cut(s) 1113
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 3 cut(s) 158, 367, 866
Bsp1720I GCTNAGC 1 cut(s) 1422
BspACI CCGC 7 cut(s) 23, 255, 258, 261, 272, 276, 314
BspANI GGCC 6 cut(s) 49, 96, 403, 835, 957, 1082
BspCNI CTCAG 1 cut(s) 501
BspFNI CGCG 2 cut(s) 274, 276
BspHI TCATGA 2 cut(s) 841, 1128
BspLI GGNNCC 7 cut(s) 76, 549, 556, 799, 834, 1462, 1546
BspMAI CTGCAG 1 cut(s) 481
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 2 cut(s) 166, 874
BspT107I GGYRCC 2 cut(s) 547, 1544
BspTNI GGTCTC 1 cut(s) 1113
BsrDI GCAATG 1 cut(s) 1490
BsrI ACTGG 4 cut(s) 135, 600, 625, 712
BssECI CCNNGG 4 cut(s) 9, 405, 1077, 1539
BssMI GATC 3 cut(s) 158, 367, 866
BssNI GRCGYC 1 cut(s) 1317
BssT1I CCWWGG 1 cut(s) 1077
Bst2UI CCWGG 4 cut(s) 519, 552, 1026, 1232
Bst4CI ACNGT 3 cut(s) 465, 913, 1193
Bst6I CTCTTC 3 cut(s) 374, 825, 942
BstACI GRCGYC 1 cut(s) 1317
BstC8I GCNNGC 5 cut(s) 130, 1009, 1369, 1398, 1428
BstDEI CTNAG 4 cut(s) 37, 509, 543, 1422
BstENI CCTNNNNNAGG 1 cut(s) 409
BstFNI CGCG 2 cut(s) 274, 276
BstHHI GCGC 2 cut(s) 276, 727
BstKTI GATC 3 cut(s) 161, 370, 869
BstMAI GTCTC 2 cut(s) 1113, 1144
BstMBI GATC 3 cut(s) 158, 367, 866
BstMWI GCNNNNNNNGC 4 cut(s) 11, 172, 1142, 1427
BstNI CCWGG 4 cut(s) 519, 552, 1026, 1232
BstNSI RCATGY 1 cut(s) 1421
BstSCI CCNGG 6 cut(s) 100, 517, 550, 576, 1024, 1230
BstSFI CTRYAG 2 cut(s) 477, 879
BstUI CGCG 2 cut(s) 274, 276
BstV1I GCAGC 7 cut(s) 17, 463, 514, 553, 1075, 1387, 1417
BsuRI GGCC 6 cut(s) 49, 96, 403, 835, 957, 1082
BtsIMutI CAGTG 6 cut(s) 154, 607, 618, 684, 857, 1273
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 5 cut(s) 130, 1009, 1369, 1398, 1428
CciI TCATGA 2 cut(s) 841, 1128
CfoI GCGC 2 cut(s) 276, 727
Cfr13I GGNCC 7 cut(s) 47, 74, 402, 833, 956, 1338, 1461
CseI GACGC 2 cut(s) 1128, 1325
CsiI ACCWGGT 1 cut(s) 1024
Csp6I GTAC 2 cut(s) 138, 961
CviAII CATG 6 cut(s) 169, 560, 803, 842, 1129, 1418
CviQI GTAC 2 cut(s) 138, 961
DdeI CTNAG 4 cut(s) 37, 509, 543, 1422
DpnI GATC 3 cut(s) 160, 369, 868
DpnII GATC 3 cut(s) 158, 367, 866
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 3 cut(s) 374, 825, 942
EarI CTCTTC 3 cut(s) 374, 825, 942
EciI GGCGGA 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 1077
Eco31I GGTCTC 1 cut(s) 1113
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 3 cut(s) 74, 1338, 1461
Eco57I CTGAAG 2 cut(s) 72, 272
EcoNI CCTNNNNNAGG 1 cut(s) 409
EcoO109I RGGNCCY 2 cut(s) 47, 833
EcoRII CCWGG 4 cut(s) 517, 550, 1024, 1230
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 1077
EcoT22I ATGCAT 1 cut(s) 1492
ErhI CCWWGG 1 cut(s) 1077
FaeI CATG 6 cut(s) 172, 563, 806, 845, 1132, 1421
FaqI GGGAC 5 cut(s) 227, 253, 560, 1058, 1474
FatI CATG 6 cut(s) 168, 559, 802, 841, 1128, 1417
FauI CCCGC 1 cut(s) 279
FblI GTMKAC 1 cut(s) 345
GlaI GCGC 2 cut(s) 275, 726
GsuI CTGGAG 3 cut(s) 118, 540, 1095
HaeIII GGCC 6 cut(s) 49, 96, 403, 835, 957, 1082
HapII CCGG 3 cut(s) 102, 351, 578
HgaI GACGC 2 cut(s) 1128, 1325
HhaI GCGC 2 cut(s) 276, 727
Hin1I GRCGYC 1 cut(s) 1317
Hin1II CATG 6 cut(s) 172, 563, 806, 845, 1132, 1421
Hin6I GCGC 2 cut(s) 274, 725
HinP1I GCGC 2 cut(s) 274, 725
HincII GTYRAC 3 cut(s) 346, 641, 1162
HindII GTYRAC 3 cut(s) 346, 641, 1162
HinfI GANTC 9 cut(s) 185, 502, 581, 592, 637, 767, 979, 1250, 1298
HpaII CCGG 3 cut(s) 102, 351, 578
HphI GGTGA 4 cut(s) 187, 244, 1040, 1245
Hpy166II GTNNAC 4 cut(s) 346, 443, 641, 1162
Hpy188I TCNGA 8 cut(s) 291, 424, 733, 871, 974, 1149, 1279, 1471
Hpy8I GTNNAC 4 cut(s) 346, 443, 641, 1162
Hpy99I CGWCG 3 cut(s) 347, 428, 454
HpyAV CCTTC 6 cut(s) 47, 580, 648, 800, 909, 1525
HpyCH4III ACNGT 3 cut(s) 465, 913, 1193
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 9 cut(s) 479, 530, 569, 682, 930, 1361, 1388, 1403, 1490
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 172, 1142, 1427
HpyF3I CTNAG 4 cut(s) 37, 509, 543, 1422
HpySE526I ACGT 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 1317
Hsp92II CATG 6 cut(s) 172, 563, 806, 845, 1132, 1421
HspAI GCGC 2 cut(s) 274, 725
Kzo9I GATC 3 cut(s) 158, 367, 866
LmnI GCTCC 5 cut(s) 25, 137, 425, 560, 1150
Lsp1109I GCAGC 7 cut(s) 17, 463, 514, 553, 1075, 1387, 1417
LweI GCATC 1 cut(s) 463
MabI ACCWGGT 1 cut(s) 1024
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 8 cut(s) 86, 149, 328, 496, 755, 1016, 1093, 1268
MalI GATC 3 cut(s) 160, 369, 868
MboI GATC 3 cut(s) 158, 367, 866
MboII GAAGA 9 cut(s) 278, 391, 812, 929, 1195, 1222, 1338, 1516, 1540
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 1 cut(s) 177
MluCI AATT 7 cut(s) 143, 371, 397, 531, 688, 992, 1085
MlyI GAGTC 1 cut(s) 646
MmeI TCCRAC 1 cut(s) 447
Mph1103I ATGCAT 1 cut(s) 1492
MseI TTAA 1 cut(s) 1173
MspI CCGG 3 cut(s) 102, 351, 578
MspR9I CCNGG 6 cut(s) 102, 519, 552, 578, 1026, 1232
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 1492
MvaI CCWGG 4 cut(s) 519, 552, 1026, 1232
MvnI CGCG 2 cut(s) 274, 276
MwoI GCNNNNNNNGC 4 cut(s) 11, 172, 1142, 1427
NciI CCSGG 2 cut(s) 102, 578
NdeII GATC 3 cut(s) 158, 367, 866
NlaIII CATG 6 cut(s) 172, 563, 806, 845, 1132, 1421
NlaIV GGNNCC 7 cut(s) 76, 549, 556, 799, 834, 1462, 1546
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 4 cut(s) 149, 328, 1093, 1268
NsiI ATGCAT 1 cut(s) 1492
NspI RCATGY 1 cut(s) 1421
PagI TCATGA 2 cut(s) 841, 1128
PcsI WCGNNNNNNNCGW 1 cut(s) 1145
PctI GAATGC 1 cut(s) 1492
PfeI GAWTC 8 cut(s) 185, 502, 581, 592, 767, 979, 1250, 1298
PfoI TCCNGGA 2 cut(s) 517, 576
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
Psp6I CCWGG 4 cut(s) 517, 550, 1024, 1230
PspGI CCWGG 4 cut(s) 517, 550, 1024, 1230
PspN4I GGNNCC 7 cut(s) 76, 549, 556, 799, 834, 1462, 1546
PspPI GGNCC 7 cut(s) 47, 74, 402, 833, 956, 1338, 1461
PstI CTGCAG 1 cut(s) 481
RsaI GTAC 2 cut(s) 139, 962
RsaNI GTAC 2 cut(s) 138, 961
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 1173
Sau3AI GATC 3 cut(s) 158, 367, 866
Sau96I GGNCC 7 cut(s) 47, 74, 402, 833, 956, 1338, 1461
SchI GAGTC 1 cut(s) 646
ScrFI CCNGG 6 cut(s) 102, 519, 552, 578, 1026, 1232
SduI GDGCHC 1 cut(s) 177
SexAI ACCWGGT 1 cut(s) 1024
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 2 cut(s) 477, 879
SinI GGWCC 3 cut(s) 74, 1338, 1461
SmlI CTYRAG 2 cut(s) 941, 1326
SmoI CTYRAG 2 cut(s) 941, 1326
Sse9I AATT 7 cut(s) 143, 371, 397, 531, 688, 992, 1085
SsiI CCGC 7 cut(s) 23, 255, 258, 261, 272, 276, 314
StyD4I CCNGG 6 cut(s) 100, 517, 550, 576, 1024, 1230
StyI CCWWGG 1 cut(s) 1077
TaaI ACNGT 3 cut(s) 465, 913, 1193
TaiI ACGT 1 cut(s) 345
TaqI TCGA 9 cut(s) 30, 43, 114, 161, 345, 488, 584, 652, 667
TasI AATT 7 cut(s) 143, 371, 397, 531, 688, 992, 1085
TauI GCSGC 4 cut(s) 260, 263, 279, 316
TfiI GAWTC 8 cut(s) 185, 502, 581, 592, 767, 979, 1250, 1298
Tru1I TTAA 1 cut(s) 1173
Tru9I TTAA 1 cut(s) 1173
TscAI CASTG 6 cut(s) 154, 607, 625, 684, 857, 1273
TseFI GTSAC 4 cut(s) 149, 328, 1093, 1268
TseI GCWGC 7 cut(s) 5, 476, 527, 566, 1063, 1400, 1430
Tsp45I GTSAC 4 cut(s) 149, 328, 1093, 1268
TspDTI ATGAA 5 cut(s) 663, 819, 830, 1117, 1185
TspGWI ACGGA 2 cut(s) 87, 178
TspRI CASTG 6 cut(s) 154, 607, 625, 684, 857, 1273
VpaK11BI GGWCC 3 cut(s) 74, 1338, 1461
XagI CCTNNNNNAGG 1 cut(s) 409
XapI RAATTY 2 cut(s) 143, 992
XceI RCATGY 1 cut(s) 1421
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.