MD15G1424000.v1.1

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010315
Physical Location & Seq
Reverse (-)
52496944 .. 52502107
5164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1424000.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGGCAACTGCGGAACCTGCCCCGCTTTTGGATATCTTAGTTCGAGGACCAGAAGGATTCTCACTCTGGAATGGGCCACCGTTTAGCAATGGGCAACCCGGTGTCAAGCTTGAAAACGTTTCCTGCTCGAGTACCAAATTCAGTGAAGATGGATCGACGCTCATGGTGATAAAATCTGATTCAGTGATTAGTATATATGACTGTACCAGCTACAGGGAGATCAGATCTTTCCAAATCCCAAATGTTCTTGCAGCTGCCTTGTCCCCACGTGGAACGTATCTTCAAACATTCCAAAAATTCTCAACACCGCAGGAGAAGAATGTCACCTTATGGAGGACGGACACTGGTGATTCCGTTTATCATCAACACCAAAAGAACATGACAAAAGCTACATGGCCCTCAATTAAATTCAGCTTTGATGAAGCTGTTGCTTGTCGACTGGCGACAAATGAGATACAATTTTTTGATGTTGGGGATTTCTCTAAAGCATTTATAAATCGGCTAAGAGTTCCTGGAGTGGCTGCTCTTGAGCTTTCTAAGGCACCCGGCTCCCATGTGGCAGCTTTTGTTCCAGAATCCAAGGGGATTCCTGCCAGTATCCAGATATTTGCTTGTGGAAAAGATCCACAGAGTCAACCTGTTGCTCGACGGAGTTTCTTCCGATGTTCAACAGTACAACTGAACTGGAACAATGGCTCAACTGGGCTTTTAGTTTTGGTGCAGTCAGATGTTGATAAAACCAATCAGAGTTACTATGGGGAATCAAAGCTCAATTACTTAACAACAGATGGGAGCCATGAAGGACTTGTACCTCTTCGTAAAGAAGGGCCTGTTCATGATGTTCAGTGGTCACATTCTGGATCAGAATTTGCCGTAGTTTATGGCTTCATGCCTGCAAAAGCTACCGTGTTTGACAAGAAGTGCAATCCTCTTCTTGAGCTTGGAACAGGCCCATACAATACTATCCGATGGAATCCAAAGGGAAAATTTCTGTGTTTGGCAGGCTTTGGTAACTTACCTGGTGATATGGCATTTTGGGACTATATAGATAAAAAACAGCTTGGGGCAACTAGAGCCGAACTGTCTGTGACAAGCGAATGGTCTCCAGATGGACGTTATTTCATGACTGCAACAACAGCTCCCAGACTACAAGTTGACAATGGGATTAAGATTTTCCACTACAACGGATCATTATTCTTCAAGAAGATGTTTGACAAGTTGTACCAGGTTGATTGGAAACCGGAGTCACCAGACAGATTCGGTGACATTGCTGAACTTGTCAAGTCTATTGAGTCATTAAAAGTAGTAGAAACGAAACCTCAAGGAGAAACGTCAAAAAATTCTCAAACTTCTGTAAAAGCTAGCACTAGCAACCCTCCTGCACAAAAGCCAGCTGCATATCGGCCCCCACACGCTAAGGCTGCCGCTGCTGTTCAGGCTCAGCTGTTTGGGGAGAGCCCTACGGAGACCTTGAGCAAGAATGCGTTGCGAAACAAGAAGAAAAGGGAGAAGAAAAAGGCTGGTGAGGCTACCAGTGGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF2A

Protein Analysis

515

Amino Acids

56.59

Weight (kDa)

9.1

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 217 - 410 7.2e-78 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73180 AT1G73180
fragaria_vesca FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500
malus_domestica MD15G1424000.v1.1
prunus_persica Prupe.1G207000_v2.0.a1
pyrus_communis pycom15g37600
rosa_chinensis RchiOBHm_Chr2g0123631
rosa_laevigata RLG00000018761
rosa_multiflora Rmu_ssc0000091.1_g000025
rosa_roxburghii Rroxscaffold_2G00120520
rosa_rugosa Rorug02G0246500 Rorug02G0246600
rosa_samantha Rh2AG305800 Rh2BG313800 Rh2CG291900 Rh2DG329600
rosa_wichuraiana Rw2G024520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
Acc36I ACCTGC 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 541
AccI GTMKAC 1 cut(s) 436
AciI CCGC 4 cut(s) 11, 23, 308, 1425
AclI AACGTT 1 cut(s) 117
AclWI GGATC 4 cut(s) 160, 617, 868, 1195
AcsI RAATTY 6 cut(s) 137, 296, 407, 866, 986, 1339
AcvI CACGTG 1 cut(s) 269
AfaI GTAC 5 cut(s) 133, 205, 675, 810, 1223
AfiI CCNNNNNNNGG 3 cut(s) 28, 213, 333
AgsI TTSAA 4 cut(s) 113, 284, 669, 1201
AjnI CCWGG 3 cut(s) 511, 1018, 1224
Alw26I GTCTC 2 cut(s) 1107, 1460
AlwI GGATC 4 cut(s) 160, 617, 868, 1195
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 5 cut(s) 74, 395, 827, 949, 1403
ApeKI GCWGC 7 cut(s) 251, 254, 521, 560, 1394, 1421, 1427
ApoI RAATTY 6 cut(s) 137, 296, 407, 866, 986, 1339
AspLEI GCGC 1 cut(s) 1541
AspS9I GGNCC 6 cut(s) 47, 74, 396, 827, 950, 1404
AsuC2I CCSGG 2 cut(s) 99, 546
AsuHPI GGTGA 7 cut(s) 178, 316, 359, 1034, 1239, 1274, 1535
AsuNHI GCTAGC 1 cut(s) 1361
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 1 cut(s) 47
BanI GGYRCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 1460
BarI GAAGNNNNNNTAC 2 cut(s) 792, 824
BbrPI CACGTG 1 cut(s) 269
BbvI GCAGC 7 cut(s) 241, 263, 508, 572, 1381, 1408, 1414
BccI CCATC 4 cut(s) 143, 782, 963, 1103
BceAI ACGGC 1 cut(s) 857
BciT130I CCWGG 3 cut(s) 513, 1020, 1226
BciVI GTATCC 1 cut(s) 608
BcnI CCSGG 2 cut(s) 99, 546
BcoDI GTCTC 2 cut(s) 1107, 1460
BfaI CTAG 3 cut(s) 1071, 1362, 1368
BfmI CTRYAG 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 1542
BfuAI ACCTGC 1 cut(s) 25
BfuI GTATCC 1 cut(s) 608
BglII AGATCT 1 cut(s) 224
BisI GCNGC 8 cut(s) 252, 255, 522, 561, 1395, 1422, 1425, 1428
BlpI GCTNAGC 1 cut(s) 1440
BlsI GCNGC 8 cut(s) 253, 256, 523, 562, 1396, 1423, 1426, 1429
Bme1390I CCNGG 5 cut(s) 99, 513, 546, 1020, 1226
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 6 cut(s) 47, 74, 396, 827, 950, 1404
BmiI GGNNCC 5 cut(s) 15, 543, 550, 794, 1406
BmrFI CCNGG 5 cut(s) 99, 513, 546, 1020, 1226
BmrI ACTGGG 1 cut(s) 711
BmtI GCTAGC 1 cut(s) 1365
BmuI ACTGGG 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 534, 1089
Bpu10I CCTNAGC 1 cut(s) 1416
Bpu1102I GCTNAGC 1 cut(s) 1440
BpuEI CTTGAG 4 cut(s) 548, 956, 1305, 1492
BpuMI CCSGG 2 cut(s) 99, 546
BsaAI YACGTR 1 cut(s) 269
BsaI GGTCTC 2 cut(s) 1107, 1460
BsaJI CCNNGG 1 cut(s) 579
BsaWI WCCGGW 1 cut(s) 1240
BsaXI ACNNNNNCTCC 2 cut(s) 1123, 1153
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 213, 333
Bse1I ACTGG 6 cut(s) 349, 444, 594, 689, 706, 1533
Bse3DI GCAATG 2 cut(s) 94, 1266
BseBI CCWGG 3 cut(s) 513, 1020, 1226
BseDI CCNNGG 1 cut(s) 579
BseLI CCNNNNNNNGG 3 cut(s) 28, 213, 333
BseMI GCAATG 2 cut(s) 94, 1266
BseMII CTCAG 1 cut(s) 1454
BseNI ACTGG 6 cut(s) 349, 444, 594, 689, 706, 1533
BseXI GCAGC 7 cut(s) 241, 263, 508, 572, 1381, 1408, 1414
BsgI GTGCAG 2 cut(s) 740, 1365
BshFI GGCC 5 cut(s) 76, 397, 829, 951, 1405
BshNI GGYRCC 1 cut(s) 541
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 3 cut(s) 99, 546, 1241
BslFI GGGAC 2 cut(s) 247, 1052
BslI CCNNNNNNNGG 3 cut(s) 28, 213, 333
BsmAI GTCTC 2 cut(s) 1107, 1460
BsmFI GGGAC 2 cut(s) 247, 1052
BsmI GAATGC 1 cut(s) 1486
BsnI GGCC 5 cut(s) 76, 397, 829, 951, 1405
Bso31I GGTCTC 2 cut(s) 1107, 1460
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 1460
Bsp143I GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
Bsp1720I GCTNAGC 1 cut(s) 1440
BspACI CCGC 4 cut(s) 11, 23, 308, 1425
BspANI GGCC 5 cut(s) 76, 397, 829, 951, 1405
BspCNI CTCAG 1 cut(s) 1453
BspHI TCATGA 2 cut(s) 835, 1122
BspLI GGNNCC 5 cut(s) 15, 543, 550, 794, 1406
BspMI ACCTGC 1 cut(s) 25
BspOI GCTAGC 1 cut(s) 1365
BspPI GGATC 4 cut(s) 160, 617, 868, 1195
BspT107I GGYRCC 1 cut(s) 541
BspTNI GGTCTC 2 cut(s) 1107, 1460
BsrDI GCAATG 2 cut(s) 94, 1266
BsrI ACTGG 6 cut(s) 349, 444, 594, 689, 706, 1533
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
BssT1I CCWWGG 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 513, 1020, 1226
Bst4CI ACNGT 5 cut(s) 81, 203, 673, 907, 1083
Bst6I CTCTTC 2 cut(s) 819, 936
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 4 cut(s) 894, 1003, 1363, 1392
BstDEI CTNAG 5 cut(s) 37, 503, 537, 1416, 1440
BstENI CCTNNNNNAGG 1 cut(s) 331
BstH2I RGCGCY 1 cut(s) 1542
BstHHI GCGC 1 cut(s) 1541
BstKTI GATC 6 cut(s) 155, 222, 227, 625, 863, 1190
BstMAI GTCTC 2 cut(s) 1107, 1460
BstMBI GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
BstMWI GCNNNNNNNGC 7 cut(s) 17, 1073, 1136, 1421, 1427, 1436, 1526
BstNI CCWGG 3 cut(s) 513, 1020, 1226
BstSCI CCNGG 5 cut(s) 97, 511, 544, 1018, 1224
BstSFI CTRYAG 1 cut(s) 211
BstV1I GCAGC 7 cut(s) 241, 263, 508, 572, 1381, 1408, 1414
BstX2I RGATCY 2 cut(s) 224, 622
BstYI RGATCY 2 cut(s) 224, 622
BsuI GTATCC 1 cut(s) 608
BsuRI GGCC 5 cut(s) 76, 397, 829, 951, 1405
BtsIMutI CAGTG 5 cut(s) 148, 189, 342, 851, 1540
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 4 cut(s) 894, 1003, 1363, 1392
CciI TCATGA 2 cut(s) 835, 1122
CfoI GCGC 1 cut(s) 1541
Cfr13I GGNCC 6 cut(s) 47, 74, 396, 827, 950, 1404
CseI GACGC 1 cut(s) 166
CsiI ACCWGGT 2 cut(s) 1018, 1224
Csp6I GTAC 5 cut(s) 132, 204, 674, 809, 1222
CviAII CATG 8 cut(s) 163, 379, 393, 554, 797, 836, 889, 1123
CviQI GTAC 5 cut(s) 132, 204, 674, 809, 1222
DdeI CTNAG 5 cut(s) 37, 503, 537, 1416, 1440
DpnI GATC 6 cut(s) 154, 221, 226, 624, 862, 1189
DpnII GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
Eam1104I CTCTTC 2 cut(s) 819, 936
EarI CTCTTC 2 cut(s) 819, 936
Eco130I CCWWGG 1 cut(s) 579
Eco24I GRGCYC 1 cut(s) 1460
Eco31I GGTCTC 2 cut(s) 1107, 1460
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 47
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 127
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 827
EcoRII CCWGG 3 cut(s) 511, 1018, 1224
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 579
EcoT38I GRGCYC 1 cut(s) 1460
ErhI CCWWGG 1 cut(s) 579
FaeI CATG 8 cut(s) 166, 382, 396, 557, 800, 839, 892, 1126
FaqI GGGAC 2 cut(s) 247, 1052
FatI CATG 8 cut(s) 162, 378, 392, 553, 796, 835, 888, 1122
FauI CCCGC 1 cut(s) 30
FblI GTMKAC 1 cut(s) 436
Fnu4HI GCNGC 8 cut(s) 252, 255, 522, 561, 1395, 1422, 1425, 1428
FriOI GRGCYC 1 cut(s) 1460
Fsp4HI GCNGC 8 cut(s) 252, 255, 522, 561, 1395, 1422, 1425, 1428
FspBI CTAG 3 cut(s) 1071, 1362, 1368
GlaI GCGC 1 cut(s) 1540
GluI GCNGC 8 cut(s) 252, 255, 522, 561, 1395, 1422, 1425, 1428
GsuI CTGGAG 2 cut(s) 534, 1089
HaeII RGCGCY 1 cut(s) 1542
HaeIII GGCC 5 cut(s) 76, 397, 829, 951, 1405
HapII CCGG 3 cut(s) 99, 546, 1241
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 1541
Hin1II CATG 8 cut(s) 166, 382, 396, 557, 800, 839, 892, 1126
Hin6I GCGC 1 cut(s) 1539
HinP1I GCGC 1 cut(s) 1539
HincII GTYRAC 3 cut(s) 437, 635, 1156
HindII GTYRAC 3 cut(s) 437, 635, 1156
HindIII AAGCTT 1 cut(s) 107
HpaII CCGG 3 cut(s) 99, 546, 1241
HphI GGTGA 7 cut(s) 178, 316, 359, 1034, 1239, 1274, 1535
Hpy166II GTNNAC 3 cut(s) 437, 635, 1156
Hpy188I TCNGA 7 cut(s) 178, 224, 662, 727, 747, 865, 968
Hpy8I GTNNAC 3 cut(s) 437, 635, 1156
Hpy99I CGWCG 2 cut(s) 160, 651
HpyAV CCTTC 3 cut(s) 47, 794, 818
HpyCH4III ACNGT 5 cut(s) 81, 203, 673, 907, 1083
HpyCH4IV ACGT 5 cut(s) 117, 268, 275, 1114, 1331
HpyCH4V TGCA 7 cut(s) 251, 721, 896, 924, 1130, 1382, 1397
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 1073, 1136, 1421, 1427, 1436, 1526
HpyF3I CTNAG 5 cut(s) 37, 503, 537, 1416, 1440
HpySE526I ACGT 5 cut(s) 117, 268, 275, 1114, 1331
Hsp92II CATG 8 cut(s) 166, 382, 396, 557, 800, 839, 892, 1126
HspAI GCGC 1 cut(s) 1539
Kzo9I GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
LmnI GCTCC 3 cut(s) 554, 792, 1144
Lsp1109I GCAGC 7 cut(s) 241, 263, 508, 572, 1381, 1408, 1414
MabI ACCWGGT 2 cut(s) 1018, 1224
MaeI CTAG 3 cut(s) 1071, 1362, 1368
MaeII ACGT 5 cut(s) 117, 268, 275, 1114, 1331
MaeIII GTNAC 7 cut(s) 322, 749, 849, 1010, 1087, 1245, 1262
MalI GATC 6 cut(s) 154, 221, 226, 624, 862, 1189
MboI GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
MflI RGATCY 2 cut(s) 224, 622
MhlI GDGCHC 1 cut(s) 1460
MluCI AATT 9 cut(s) 137, 296, 402, 407, 458, 772, 866, 986, 1339
MlyI GAGTC 3 cut(s) 640, 1253, 1301
MnlI CCTC 8 cut(s) 38, 327, 409, 822, 939, 1329, 1386, 1519
MseI TTAA 4 cut(s) 405, 779, 1167, 1298
MspA1I CMGCKG 4 cut(s) 254, 1394, 1427, 1444
MspI CCGG 3 cut(s) 99, 546, 1241
MspR9I CCNGG 5 cut(s) 99, 513, 546, 1020, 1226
Mva1269I GAATGC 1 cut(s) 1486
MvaI CCWGG 3 cut(s) 513, 1020, 1226
MwoI GCNNNNNNNGC 7 cut(s) 17, 1073, 1136, 1421, 1427, 1436, 1526
NciI CCSGG 2 cut(s) 99, 546
NdeII GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
NheI GCTAGC 1 cut(s) 1361
NlaIII CATG 8 cut(s) 166, 382, 396, 557, 800, 839, 892, 1126
NlaIV GGNNCC 5 cut(s) 15, 543, 550, 794, 1406
NmuCI GTSAC 5 cut(s) 322, 849, 1087, 1245, 1262
PaeR7I CTCGAG 1 cut(s) 127
PagI TCATGA 2 cut(s) 835, 1122
PctI GAATGC 1 cut(s) 1486
PfeI GAWTC 8 cut(s) 57, 179, 350, 575, 586, 761, 973, 1257
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 8 cut(s) 253, 256, 523, 562, 1396, 1423, 1426, 1429
PleI GAGTC 3 cut(s) 639, 1252, 1300
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 3 cut(s) 639, 1252, 1300
Ppu21I YACGTR 1 cut(s) 269
PsiI TTATAA 1 cut(s) 494
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 3 cut(s) 511, 1018, 1224
PspCI CACGTG 1 cut(s) 269
PspGI CCWGG 3 cut(s) 511, 1018, 1224
PspN4I GGNNCC 5 cut(s) 15, 543, 550, 794, 1406
PspPI GGNCC 6 cut(s) 47, 74, 396, 827, 950, 1404
PspXI VCTCGAGB 1 cut(s) 127
PsuI RGATCY 2 cut(s) 224, 622
PvuII CAGCTG 3 cut(s) 254, 1394, 1444
RsaI GTAC 5 cut(s) 133, 205, 675, 810, 1223
RsaNI GTAC 5 cut(s) 132, 204, 674, 809, 1222
SalI GTCGAC 1 cut(s) 435
SaqAI TTAA 4 cut(s) 405, 779, 1167, 1298
SatI GCNGC 8 cut(s) 252, 255, 522, 561, 1395, 1422, 1425, 1428
Sau3AI GATC 6 cut(s) 152, 219, 224, 622, 860, 1187
Sau96I GGNCC 6 cut(s) 47, 74, 396, 827, 950, 1404
SchI GAGTC 3 cut(s) 640, 1253, 1301
ScrFI CCNGG 5 cut(s) 99, 513, 546, 1020, 1226
SduI GDGCHC 1 cut(s) 1460
SexAI ACCWGGT 2 cut(s) 1018, 1224
SfcI CTRYAG 1 cut(s) 211
Sfr274I CTCGAG 1 cut(s) 127
SinI GGWCC 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 127
SmlI CTYRAG 5 cut(s) 127, 527, 935, 1320, 1471
SmoI CTYRAG 5 cut(s) 127, 527, 935, 1320, 1471
Sse9I AATT 9 cut(s) 137, 296, 402, 407, 458, 772, 866, 986, 1339
SsiI CCGC 4 cut(s) 11, 23, 308, 1425
SspMI CTAG 3 cut(s) 1071, 1362, 1368
StyD4I CCNGG 5 cut(s) 97, 511, 544, 1018, 1224
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 5 cut(s) 81, 203, 673, 907, 1083
TaiI ACGT 5 cut(s) 120, 271, 278, 1117, 1334
TaqI TCGA 5 cut(s) 43, 128, 155, 436, 646
TasI AATT 9 cut(s) 137, 296, 402, 407, 458, 772, 866, 986, 1339
TatI WGTACW 1 cut(s) 673
TauI GCSGC 1 cut(s) 1427
TfiI GAWTC 8 cut(s) 57, 179, 350, 575, 586, 761, 973, 1257
Tru1I TTAA 4 cut(s) 405, 779, 1167, 1298
Tru9I TTAA 4 cut(s) 405, 779, 1167, 1298
TscAI CASTG 5 cut(s) 148, 189, 349, 851, 1540
TseFI GTSAC 5 cut(s) 322, 849, 1087, 1245, 1262
TseI GCWGC 7 cut(s) 251, 254, 521, 560, 1394, 1421, 1427
Tsp45I GTSAC 5 cut(s) 322, 849, 1087, 1245, 1262
TspDTI ATGAA 5 cut(s) 435, 813, 824, 877, 1111
TspGWI ACGGA 5 cut(s) 343, 353, 664, 1200, 1478
TspRI CASTG 5 cut(s) 148, 189, 349, 851, 1540
VpaK11BI GGWCC 1 cut(s) 47
XagI CCTNNNNNAGG 1 cut(s) 331
XapI RAATTY 6 cut(s) 137, 296, 407, 866, 986, 1339
XhoI CTCGAG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 436
XspI CTAG 3 cut(s) 1071, 1362, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.