FvH4_1g25500

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010315
Physical Location & Seq
Reverse (-)
17261513 .. 17267539
6027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25500.t3

Sequence Viewer

Length: 1710 bp
ATGGTTGTTCAAGGACCGAGTGTGGCTGCTAGGTGTGAGTTTGTCGACGACGACGACGAAGAGGGAAGAAGATGGATGCTGACGCGGAAAAAAGTGACGTGTTGGATTTCGAATAGCCGGTTGCAGAAGCCATGTGCGAGCGCCCCACCTATGGCCGCCGCGGCTCAACCTCCGCCGCCGCTCGACATCTTAGTCCGATCACCGGAAGGCTTCTCACTCTGGAACGGGCCCCCGTTTACCAACGGCCAACCCGGCATCAAGCTCGACCAGAAAATCTCTTGCTCCACCACCAAATTCAGCGACGACGGATCGCGCCTCATGGTGCTGAAGCCCGATGGCGTCATCACCATCTACGACTGCCGGAGGTACACCGAGATTAGGTCTTTCCAAGTCCCCAATCTCACCGCCGCCGCGTTGTCCCCACGCGGCAGGTTTGTCCAGACTTTCCAGAAGTCCTCGACGCCGCAGAACAAGAATGTGACGCTGTGGAACGTCGACACCGGAGAAGCGGAGTACGATCAGTTCCAGAAGAGCATAACCAGAACGAACTGGCCGTCGATTAGGTTCAGCGCCGACGAGGCGGTTGCGTGTAAACTGGCGACGAATGAGGTACAGTTTTTCGATGCTGTGGATTTTTCGAAAGGAGTTACGAATCGGTTGAGAGTTGCTGGAGTTGCTGCAATTGAGCTTTCTACGGCGCCTGGCTCTCATGTGGCTGCATTTGTTCCTGAAGCCAAGGGGATTCCTGCTAGTGTCCAGATATTTGCAACTAGAAAAGATTTACAGAGTCAACCTGTTGCTCGGAAGAGTTTCTTTCGATGCTCTTCAGTGCAACTGAAATGGAATAATGGATCAACGGGGCTTTTAGTTGTGGCGCAGTCAGATGTTGATAAAACCAACCAAAGTTACTATGGGGAATCAAAGCTGTACTACTTGACAACAGATGGAACCTATGAAGGACTTGTACCTCTTCGCAAAGAGGGGCCTGTTCATGATGTTCAGTGGTCATACTCTGGATCAGAGTTTGCTGTAGTTTATGGCTTTATGCCTTCTAAAACAACTGTGTTTGATAAGAAGTGCAATCCTCTTCTTGAGCTTGGAACAGGCCCGTACAATACTATCCGATGGAATCCAAAGGGAAAATTTCTGTGTGTGGCAGGCTTTGGTAACTTACCAGGTGATATGGCATTTTGGGACTACATGGAGAAAAAGCAGCTTGGAACAACTAAGGCCGAATTATCCGTGACAAGCGAATGGTCTCCAGATGGATGCTATTTCATGACTGCCACAACTGCTCCAAGACTACAAGTTGACAATGAGATTAAGATTTTCCACCACAATGGTTCATTGTTCTTCAAGAAGATGTTTGACAAGCTGTTCCAGGCTGATTGGAAGCCTGAGTCACCTGATAGGTTCAGTGACATTTCAGAACTTGTCAAGTCCTTTGTATCAATAAAAGTTGATAATGCGAAACCTCAAGGGGAAAGGTCAAAATCTTCCCAGACGTCTGCAAAAGCCATCCCTAACAATCCACCTGCACAAAAGCCTGCAGCATATCGGCCCCCAAATGCTAAGCAAGCTGCTATCGTTCAGGCAGAGTTGTTTGGAGGAACCTCCTCAGAAACAATGAGTAAGAATGCATTGCGAAACAAGAAGAAAAGGGAGAAACAAAAGGAGAAGAAGGCTACCGAGGGGGCCAATGATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF2A

Protein Analysis

570

Amino Acids

63.05

Weight (kDa)

9.18

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF2A PF08662 219 - 412 1e-76 Eukaryotic translation initiation factor eIF2A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73180 AT1G73180
fragaria_vesca FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500 FvH4_1g25500
malus_domestica MD15G1424000.v1.1
prunus_persica Prupe.1G207000_v2.0.a1
pyrus_communis pycom15g37600
rosa_chinensis RchiOBHm_Chr2g0123631
rosa_laevigata RLG00000018761
rosa_multiflora Rmu_ssc0000091.1_g000025
rosa_roxburghii Rroxscaffold_2G00120520
rosa_rugosa Rorug02G0246500 Rorug02G0246600
rosa_samantha Rh2AG305800 Rh2BG313800 Rh2CG291900 Rh2DG329600
rosa_wichuraiana Rw2G024520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1543
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 1508
Acc36I ACCTGC 2 cut(s) 420, 1543
AccB1I GGYRCC 1 cut(s) 697
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 2 cut(s) 45, 495
AccII CGCG 5 cut(s) 85, 161, 313, 413, 426
AclWI GGATC 3 cut(s) 316, 859, 1024
AcoI YGGCCR 3 cut(s) 153, 244, 551
AcsI RAATTY 2 cut(s) 293, 1142
AcuI CTGAAG 3 cut(s) 347, 750, 810
AcyI GRCGYC 4 cut(s) 339, 461, 698, 1505
AfaI GTAC 6 cut(s) 368, 515, 612, 929, 966, 1112
AfiI CCNNNNNNNGG 3 cut(s) 151, 202, 378
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 2 cut(s) 11, 1357
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 3 cut(s) 700, 1174, 1380
AluBI AGCT 7 cut(s) 262, 688, 925, 1096, 1216, 1375, 1580
AluI AGCT 7 cut(s) 262, 688, 925, 1096, 1216, 1375, 1580
Alw26I GTCTC 1 cut(s) 1263
AlwI GGATC 3 cut(s) 316, 859, 1024
AoxI GGCC 9 cut(s) 153, 227, 244, 551, 983, 1105, 1230, 1559, 1695
ApaI GGGCCC 1 cut(s) 231
ApeKI GCWGC 6 cut(s) 26, 677, 716, 1213, 1550, 1580
ApoI RAATTY 2 cut(s) 293, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
Asp700I GAANNNNTTC 1 cut(s) 809
AspLEI GCGC 5 cut(s) 143, 315, 572, 700, 877
AspS9I GGNCC 7 cut(s) 14, 227, 228, 983, 1106, 1560, 1695
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 5 cut(s) 192, 337, 394, 1190, 1395
AsuII TTCGAA 2 cut(s) 110, 638
AvaII GGWCC 1 cut(s) 14
BaeGI GKGCMC 1 cut(s) 231
BanI GGYRCC 1 cut(s) 697
BanII GRGCYC 1 cut(s) 231
BarI GAAGNNNNNNTAC 2 cut(s) 948, 980
BbvI GCAGC 6 cut(s) 13, 664, 703, 1225, 1562, 1567
BccI CCATC 7 cut(s) 66, 329, 356, 938, 1119, 1259, 1526
BceAI ACGGC 3 cut(s) 259, 538, 711
BcgI CGANNNNNNTGC 4 cut(s) 244, 278, 566, 600
BciT130I CCWGG 3 cut(s) 702, 1176, 1382
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 1263
BfaI CTAG 3 cut(s) 30, 750, 771
BfmI CTRYAG 2 cut(s) 1029, 1548
BfoI RGCGCY 3 cut(s) 144, 573, 701
BfuAI ACCTGC 2 cut(s) 420, 1543
BglI GCCNNNNNGGC 3 cut(s) 161, 252, 578
BlpI GCTNAGC 1 cut(s) 1572
Bme1390I CCNGG 4 cut(s) 252, 702, 1176, 1382
Bme18I GGWCC 1 cut(s) 14
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 7 cut(s) 14, 227, 228, 983, 1106, 1560, 1695
BmiI GGNNCC 8 cut(s) 229, 230, 699, 949, 984, 1562, 1612, 1696
BmrFI CCNGG 4 cut(s) 252, 702, 1176, 1382
BmsI GCATC 5 cut(s) 66, 264, 613, 809, 1259
BpmI CTGGAG 2 cut(s) 690, 1245
Bpu1102I GCTNAGC 1 cut(s) 1572
Bpu14I TTCGAA 2 cut(s) 110, 638
BpuEI CTTGAG 2 cut(s) 1112, 1461
BpuMI CCSGG 1 cut(s) 252
BsaHI GRCGYC 4 cut(s) 339, 461, 698, 1505
BsaI GGTCTC 1 cut(s) 1263
BsaJI CCNNGG 3 cut(s) 159, 735, 1689
BsaWI WCCGGW 2 cut(s) 202, 500
BsaXI ACNNNNNCTCC 2 cut(s) 1279, 1309
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 202, 378
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 2 cut(s) 554, 600
Bse3DI GCAATG 1 cut(s) 1640
BseBI CCWGG 3 cut(s) 702, 1176, 1382
BseDI CCNNGG 3 cut(s) 159, 735, 1689
BseGI GGATG 3 cut(s) 81, 1274, 1518
BseLI CCNNNNNNNGG 3 cut(s) 151, 202, 378
BseMI GCAATG 1 cut(s) 1640
BseMII CTCAG 2 cut(s) 1389, 1632
BseNI ACTGG 2 cut(s) 554, 600
BseRI GAGGAG 1 cut(s) 1606
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 6 cut(s) 13, 664, 703, 1225, 1562, 1567
BsgI GTGCAG 1 cut(s) 1521
Bsh1236I CGCG 5 cut(s) 85, 161, 313, 413, 426
BshFI GGCC 9 cut(s) 155, 229, 246, 553, 985, 1107, 1232, 1561, 1697
BshNI GGYRCC 1 cut(s) 697
BsiSI CCGG 5 cut(s) 118, 203, 252, 361, 501
BslFI GGGAC 3 cut(s) 377, 403, 1208
BslI CCNNNNNNNGG 3 cut(s) 151, 202, 378
BsmAI GTCTC 1 cut(s) 1263
BsmFI GGGAC 3 cut(s) 377, 403, 1208
BsmI GAATGC 1 cut(s) 1642
BsnI GGCC 9 cut(s) 155, 229, 246, 553, 985, 1107, 1232, 1561, 1697
Bso31I GGTCTC 1 cut(s) 1263
Bsp119I TTCGAA 2 cut(s) 110, 638
Bsp120I GGGCCC 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 231
Bsp143I GATC 5 cut(s) 197, 308, 517, 851, 1016
Bsp1720I GCTNAGC 1 cut(s) 1572
BspANI GGCC 9 cut(s) 155, 229, 246, 553, 985, 1107, 1232, 1561, 1697
BspCNI CTCAG 2 cut(s) 1390, 1631
BspFNI CGCG 5 cut(s) 85, 161, 313, 413, 426
BspHI TCATGA 2 cut(s) 991, 1278
BspLI GGNNCC 8 cut(s) 229, 230, 699, 949, 984, 1562, 1612, 1696
BspMAI CTGCAG 1 cut(s) 1552
BspMI ACCTGC 2 cut(s) 420, 1543
BspPI GGATC 3 cut(s) 316, 859, 1024
BspQI GCTCTTC 2 cut(s) 524, 829
BspT104I TTCGAA 2 cut(s) 110, 638
BspT107I GGYRCC 1 cut(s) 697
BspTNI GGTCTC 1 cut(s) 1263
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 1640
BsrFI RCCGGY 1 cut(s) 117
BsrI ACTGG 2 cut(s) 554, 600
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 159, 735, 1689
BssMI GATC 5 cut(s) 197, 308, 517, 851, 1016
BssNI GRCGYC 4 cut(s) 339, 461, 698, 1505
BssT1I CCWWGG 1 cut(s) 735
Bst2UI CCWGG 3 cut(s) 702, 1176, 1382
Bst4CI ACNGT 2 cut(s) 615, 1063
Bst6I CTCTTC 6 cut(s) 54, 524, 800, 829, 975, 1092
BstACI GRCGYC 4 cut(s) 339, 461, 698, 1505
BstBI TTCGAA 2 cut(s) 110, 638
BstC8I GCNNGC 4 cut(s) 139, 1159, 1548, 1578
BstDEI CTNAG 5 cut(s) 190, 1227, 1398, 1572, 1618
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 3 cut(s) 81, 1274, 1518
BstFNI CGCG 5 cut(s) 85, 161, 313, 413, 426
BstH2I RGCGCY 3 cut(s) 144, 573, 701
BstHHI GCGC 5 cut(s) 143, 315, 572, 700, 877
BstKTI GATC 5 cut(s) 200, 311, 520, 854, 1019
BstMAI GTCTC 1 cut(s) 1263
BstMBI GATC 5 cut(s) 197, 308, 517, 851, 1016
BstMWI GCNNNNNNNGC 6 cut(s) 161, 252, 578, 674, 1292, 1577
BstNI CCWGG 3 cut(s) 702, 1176, 1382
BstSCI CCNGG 4 cut(s) 250, 700, 1174, 1380
BstSFI CTRYAG 2 cut(s) 1029, 1548
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 5 cut(s) 85, 161, 313, 413, 426
BstV1I GCAGC 6 cut(s) 13, 664, 703, 1225, 1562, 1567
BstXI CCANNNNNNTGG 1 cut(s) 1340
BsuRI GGCC 9 cut(s) 155, 229, 246, 553, 985, 1107, 1232, 1561, 1697
BtgI CCRYGG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 99
BtsCI GGATG 3 cut(s) 81, 1274, 1518
BtsIMutI CAGTG 3 cut(s) 834, 1007, 1423
BveI ACCTGC 2 cut(s) 420, 1543
Cac8I GCNNGC 4 cut(s) 139, 1159, 1548, 1578
CciI TCATGA 2 cut(s) 991, 1278
CfoI GCGC 5 cut(s) 143, 315, 572, 700, 877
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 7 cut(s) 14, 227, 228, 983, 1106, 1560, 1695
Cfr42I CCGCGG 1 cut(s) 162
CseI GACGC 4 cut(s) 91, 328, 469, 490
CsiI ACCWGGT 1 cut(s) 1174
Csp6I GTAC 6 cut(s) 367, 514, 611, 928, 965, 1111
CviAII CATG 6 cut(s) 132, 319, 710, 992, 1201, 1279
CviQI GTAC 6 cut(s) 367, 514, 611, 928, 965, 1111
DdeI CTNAG 5 cut(s) 190, 1227, 1398, 1572, 1618
DinI GGCGCC 1 cut(s) 699
DpnI GATC 5 cut(s) 199, 310, 519, 853, 1018
DpnII GATC 5 cut(s) 197, 308, 517, 851, 1016
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 3 cut(s) 153, 244, 551
Eam1104I CTCTTC 6 cut(s) 54, 524, 800, 829, 975, 1092
EarI CTCTTC 6 cut(s) 54, 524, 800, 829, 975, 1092
EciI GGCGGA 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 1263
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 3 cut(s) 347, 750, 810
EcoO109I RGGNCCY 2 cut(s) 228, 983
EcoRII CCWGG 3 cut(s) 700, 1174, 1380
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 1 cut(s) 1642
EcoT38I GRGCYC 1 cut(s) 231
EgeI GGCGCC 1 cut(s) 699
EheI GGCGCC 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 6 cut(s) 135, 322, 713, 995, 1204, 1282
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FaqI GGGAC 3 cut(s) 377, 403, 1208
FatI CATG 6 cut(s) 131, 318, 709, 991, 1200, 1278
FblI GTMKAC 2 cut(s) 45, 495
FokI GGATG 3 cut(s) 88, 1281, 1505
FriOI GRGCYC 1 cut(s) 231
FspBI CTAG 3 cut(s) 30, 750, 771
GlaI GCGC 5 cut(s) 142, 314, 571, 699, 876
GsuI CTGGAG 2 cut(s) 690, 1245
HaeII RGCGCY 3 cut(s) 144, 573, 701
HaeIII GGCC 9 cut(s) 155, 229, 246, 553, 985, 1107, 1232, 1561, 1697
HapII CCGG 5 cut(s) 118, 203, 252, 361, 501
HgaI GACGC 4 cut(s) 91, 328, 469, 490
HhaI GCGC 5 cut(s) 143, 315, 572, 700, 877
Hin1I GRCGYC 4 cut(s) 339, 461, 698, 1505
Hin1II CATG 6 cut(s) 135, 322, 713, 995, 1204, 1282
Hin6I GCGC 5 cut(s) 141, 313, 570, 698, 875
HinP1I GCGC 5 cut(s) 141, 313, 570, 698, 875
HincII GTYRAC 4 cut(s) 46, 496, 791, 1312
HindII GTYRAC 4 cut(s) 46, 496, 791, 1312
HinfI GANTC 6 cut(s) 652, 742, 787, 917, 1129, 1400
HpaII CCGG 5 cut(s) 118, 203, 252, 361, 501
HphI GGTGA 5 cut(s) 192, 337, 394, 1190, 1395
Hpy166II GTNNAC 7 cut(s) 46, 237, 369, 496, 593, 791, 1312
Hpy188I TCNGA 7 cut(s) 197, 804, 883, 1021, 1124, 1429, 1621
Hpy8I GTNNAC 7 cut(s) 46, 237, 369, 496, 593, 791, 1312
HpyAV CCTTC 4 cut(s) 200, 950, 1059, 1675
HpyCH4III ACNGT 2 cut(s) 615, 1063
HpyCH4IV ACGT 3 cut(s) 98, 492, 1505
HpyF10VI GCNNNNNNNGC 6 cut(s) 161, 252, 578, 674, 1292, 1577
HpyF3I CTNAG 5 cut(s) 190, 1227, 1398, 1572, 1618
HpySE526I ACGT 3 cut(s) 98, 492, 1505
Hsp92I GRCGYC 4 cut(s) 339, 461, 698, 1505
Hsp92II CATG 6 cut(s) 135, 322, 713, 995, 1204, 1282
HspAI GCGC 5 cut(s) 141, 313, 570, 698, 875
KasI GGCGCC 1 cut(s) 697
KspI CCGCGG 1 cut(s) 162
Kzo9I GATC 5 cut(s) 197, 308, 517, 851, 1016
LguI GCTCTTC 2 cut(s) 524, 829
LmnI GCTCC 2 cut(s) 287, 1300
Lsp1109I GCAGC 6 cut(s) 13, 664, 703, 1225, 1562, 1567
LweI GCATC 5 cut(s) 66, 264, 613, 809, 1259
MabI ACCWGGT 1 cut(s) 1174
MaeI CTAG 3 cut(s) 30, 750, 771
MaeII ACGT 3 cut(s) 98, 492, 1505
MaeIII GTNAC 8 cut(s) 94, 478, 646, 905, 1166, 1243, 1401, 1418
MalI GATC 5 cut(s) 199, 310, 519, 853, 1018
MbiI CCGCTC 1 cut(s) 181
MboI GATC 5 cut(s) 197, 308, 517, 851, 1016
MfeI CAATTG 1 cut(s) 681
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 4 cut(s) 293, 681, 1142, 1235
Mly113I GGCGCC 1 cut(s) 698
MlyI GAGTC 2 cut(s) 796, 1409
MmeI TCCRAC 1 cut(s) 83
Mph1103I ATGCAT 1 cut(s) 1642
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 1 cut(s) 1323
MslI CAYNNNNRTG 1 cut(s) 1338
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 5 cut(s) 118, 203, 252, 361, 501
MspR9I CCNGG 4 cut(s) 252, 702, 1176, 1382
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 1 cut(s) 1642
MvaI CCWGG 3 cut(s) 702, 1176, 1382
MvnI CGCG 5 cut(s) 85, 161, 313, 413, 426
MwoI GCNNNNNNNGC 6 cut(s) 161, 252, 578, 674, 1292, 1577
NarI GGCGCC 1 cut(s) 698
NciI CCSGG 1 cut(s) 252
NdeII GATC 5 cut(s) 197, 308, 517, 851, 1016
NlaIII CATG 6 cut(s) 135, 322, 713, 995, 1204, 1282
NlaIV GGNNCC 8 cut(s) 229, 230, 699, 949, 984, 1562, 1612, 1696
NmuCI GTSAC 5 cut(s) 94, 478, 1243, 1401, 1418
NsiI ATGCAT 1 cut(s) 1642
NspV TTCGAA 2 cut(s) 110, 638
PagI TCATGA 2 cut(s) 991, 1278
PaqCI CACCTGC 1 cut(s) 1543
PciSI GCTCTTC 2 cut(s) 524, 829
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 54, 551
PctI GAATGC 1 cut(s) 1642
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 4 cut(s) 652, 742, 917, 1129
PleI GAGTC 2 cut(s) 795, 1408
PluTI GGCGCC 1 cut(s) 701
PpsI GAGTC 2 cut(s) 795, 1408
Psp6I CCWGG 3 cut(s) 700, 1174, 1380
PspGI CCWGG 3 cut(s) 700, 1174, 1380
PspN4I GGNNCC 8 cut(s) 229, 230, 699, 949, 984, 1562, 1612, 1696
PspOMI GGGCCC 1 cut(s) 227
PspPI GGNCC 7 cut(s) 14, 227, 228, 983, 1106, 1560, 1695
PsrI GAACNNNNNNTAC 2 cut(s) 506, 538
PstI CTGCAG 1 cut(s) 1552
RsaI GTAC 6 cut(s) 368, 515, 612, 929, 966, 1112
RsaNI GTAC 6 cut(s) 367, 514, 611, 928, 965, 1111
RseI CAYNNNNRTG 1 cut(s) 1338
SacII CCGCGG 1 cut(s) 162
SalI GTCGAC 2 cut(s) 44, 494
SapI GCTCTTC 2 cut(s) 524, 829
SaqAI TTAA 1 cut(s) 1323
Sau3AI GATC 5 cut(s) 197, 308, 517, 851, 1016
Sau96I GGNCC 7 cut(s) 14, 227, 228, 983, 1106, 1560, 1695
SchI GAGTC 2 cut(s) 796, 1409
ScrFI CCNGG 4 cut(s) 252, 702, 1176, 1382
SduI GDGCHC 1 cut(s) 231
SexAI ACCWGGT 1 cut(s) 1174
SfaNI GCATC 5 cut(s) 66, 264, 613, 809, 1259
SfcI CTRYAG 2 cut(s) 1029, 1548
SfoI GGCGCC 1 cut(s) 699
Sfr303I CCGCGG 1 cut(s) 162
SfuI TTCGAA 2 cut(s) 110, 638
SgrBI CCGCGG 1 cut(s) 162
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 1338
SmlI CTYRAG 2 cut(s) 1091, 1476
SmoI CTYRAG 2 cut(s) 1091, 1476
Sse9I AATT 4 cut(s) 293, 681, 1142, 1235
SspDI GGCGCC 1 cut(s) 697
SspMI CTAG 3 cut(s) 30, 750, 771
StyD4I CCNGG 4 cut(s) 250, 700, 1174, 1380
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 2 cut(s) 615, 1063
TaiI ACGT 3 cut(s) 101, 495, 1508
TaqII GACCGA 1 cut(s) 31
TasI AATT 4 cut(s) 293, 681, 1142, 1235
TatI WGTACW 1 cut(s) 927
TauI GCSGC 9 cut(s) 158, 161, 164, 178, 181, 410, 413, 429, 466
TfiI GAWTC 4 cut(s) 652, 742, 917, 1129
Tru1I TTAA 1 cut(s) 1323
Tru9I TTAA 1 cut(s) 1323
TscAI CASTG 3 cut(s) 834, 1007, 1423
TseFI GTSAC 5 cut(s) 94, 478, 1243, 1401, 1418
TseI GCWGC 6 cut(s) 26, 677, 716, 1213, 1550, 1580
Tsp45I GTSAC 5 cut(s) 94, 478, 1243, 1401, 1418
TspDTI ATGAA 4 cut(s) 969, 980, 1267, 1335
TspGWI ACGGA 2 cut(s) 321, 1231
TspRI CASTG 3 cut(s) 834, 1007, 1423
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 2 cut(s) 293, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 908
XmiI GTMKAC 2 cut(s) 45, 495
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 3 cut(s) 30, 750, 771
ZraI GACGTC 1 cut(s) 1506
Zsp2I ATGCAT 1 cut(s) 1642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.