AT1G74210

phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007756
Physical Location & Seq
Forward (+)
27910314 .. 27912941
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74210.1

Sequence Viewer

Length: 1179 bp
ATGATTCTTACAAGATGCTTACCCTTGATATGGCTATCTCTACTTACTGTGTGTGCTGCTGGGAGGACATTGCACCCTCTCCCTGTTAAAGGACCCAAAACTGTCAAGCTTCAACTTCAGACTTCACGTCCTTATAACATCGCACACAGAGGTTCCAATGGAGAGATCCCAGAAGAAACTACAGCTGCATACTTGAAAGCAATTGAAGAAGGCACAGACTTCATAGAAACAGATATCTTATCATCCAAAGATGGTGTGCTCATATGTTTCCATGATTGTATTCTCGACGAAACAACCAATGTTGCGAGCCACAAGGAGTTTGCAGATCGTAAAAGGACATATGATGTCCAAGGATTCAACATTACTGGCTTTTTCACTTTTGATTTTACACTTAAAGAACTAAAGCAACTACGTATAAAGCAGCGATATGCTTTTCGGGATCAGCAATACAACGGAATGTACCCTATCATTACATTTGAGGAATTTCTTACCATTGCTCGGGATGCTCCAAGAGTTGTTGGAATATATCCTGAGATTAAAAATCCAGTTTTAATGAACCAGCATGTCAAATGGCCTGGTGGTAAGAAATTTGAGGATAAAGTTGTGGAGACACTTAAGAAGTATGGGTATGGAGGCTCGTATTTGTCCAAGAAGTGGTTGAAAAAACCACTATTTATTCAGTCATTTGCCCCAACTTCACTCGTGTACATATCAAACTTGACAGACTCGCCGAAAGTCCTTCTAATAGACGATGTTACCATGCCAACGCAAGACACTAACCAGACATATGCAGAGATCACATCAGATGCGTATTTCGAATACATCAAGCAATATGTTGTGGGTATCGGACCATGGAAGGATACAATTGTTCCGGTAAACAACAACTATGTGCTAGCCCCCACAGATTTGGTCAAAAGAGCTCACGCACACAATCTGCAGGTGCATCCCTATACGTACAGGAATGAACACGAATTCTTACACTATAACTTTAGCCAAGACCCGTATAAGGAGTACGACTACTGGATCAATGAGATTGGAGTTGATGGACTCTTCACTGATTTCACAGGCAGCCTCCATAACTTTCAAGAATGGACATCTCCATTACCTGATACCTCCAAGTCTCCACGACAGCTTCTTAGTCAAATTGCCTCATTGGTCCTCCCTTATGCAAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00564 map00564
Pfam Domains
Protein Families
GDPD

Protein Analysis

392

Amino Acids

45.02

Weight (kDa)

6.6

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 49 - 356 1.7e-54 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 135
AarI CACCTGC 1 cut(s) 928
Acc36I ACCTGC 1 cut(s) 928
AccB7I CCANNNNNTGG 1 cut(s) 654
AclWI GGATC 3 cut(s) 160, 447, 1031
AcsI RAATTY 3 cut(s) 482, 587, 971
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 4 cut(s) 461, 707, 956, 1013
AfiI CCNNNNNNNGG 5 cut(s) 30, 89, 498, 654, 1006
AflII CTTAAG 1 cut(s) 614
AgsI TTSAA 6 cut(s) 113, 196, 206, 358, 661, 1085
AhdI GACNNNNNGTC 1 cut(s) 126
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 4 cut(s) 109, 185, 920, 1132
AluI AGCT 4 cut(s) 109, 185, 920, 1132
Alw21I GWGCWC 2 cut(s) 261, 922
Alw26I GTCTC 2 cut(s) 602, 1125
AlwI GGATC 3 cut(s) 160, 447, 1031
Ama87I CYCGRG 1 cut(s) 498
AoxI GGCC 1 cut(s) 572
ApeKI GCWGC 4 cut(s) 56, 185, 421, 1068
ApoI RAATTY 3 cut(s) 482, 587, 971
AspS9I GGNCC 3 cut(s) 92, 848, 1156
AsuII TTCGAA 1 cut(s) 816
AsuNHI GCTAGC 1 cut(s) 892
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 3 cut(s) 92, 848, 1156
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BanII GRGCYC 1 cut(s) 922
BauI CACGAG 1 cut(s) 701
Bbv12I GWGCWC 2 cut(s) 261, 922
BbvI GCAGC 4 cut(s) 43, 172, 433, 1080
BccI CCATC 2 cut(s) 245, 1037
BciT130I CCWGG 1 cut(s) 576
BciVI GTATCC 1 cut(s) 853
BcoDI GTCTC 2 cut(s) 602, 1125
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 2 cut(s) 180, 935
BfrI CTTAAG 1 cut(s) 614
BfuAI ACCTGC 1 cut(s) 928
BfuI GTATCC 1 cut(s) 853
BisI GCNGC 4 cut(s) 57, 186, 422, 1069
BlsI GCNGC 4 cut(s) 58, 187, 423, 1070
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 3 cut(s) 92, 848, 1156
BmeRI GACNNNNNGTC 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 498
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 3 cut(s) 92, 848, 1156
BmiI GGNNCC 2 cut(s) 94, 154
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 4 cut(s) 5, 493, 796, 952
BmtI GCTAGC 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 816
BsaAI YACGTR 2 cut(s) 413, 954
BsaJI CCNNGG 2 cut(s) 349, 851
BsaWI WCCGGW 1 cut(s) 871
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 89, 498, 654, 1006
Bse1I ACTGG 3 cut(s) 370, 545, 1025
Bse3DI GCAATG 2 cut(s) 68, 492
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 349, 851
BseGI GGATG 3 cut(s) 242, 508, 943
BseLI CCNNNNNNNGG 5 cut(s) 30, 89, 498, 654, 1006
BseMI GCAATG 2 cut(s) 68, 492
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 3 cut(s) 370, 545, 1025
BseXI GCAGC 4 cut(s) 43, 172, 433, 1080
BseYI CCCAGC 1 cut(s) 59
BshFI GGCC 1 cut(s) 574
BsiHKAI GWGCWC 2 cut(s) 261, 922
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 872
BslI CCNNNNNNNGG 5 cut(s) 30, 89, 498, 654, 1006
BsmAI GTCTC 2 cut(s) 602, 1125
BsnI GGCC 1 cut(s) 574
BsoBI CYCGRG 1 cut(s) 498
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 2 cut(s) 261, 922
Bsp1407I TGTACA 1 cut(s) 705
Bsp143I GATC 5 cut(s) 165, 325, 439, 795, 1023
Bsp19I CCATGG 1 cut(s) 851
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 94, 154
BspMAI CTGCAG 1 cut(s) 939
BspMI ACCTGC 1 cut(s) 928
BspOI GCTAGC 1 cut(s) 896
BspPI GGATC 3 cut(s) 160, 447, 1031
BspT104I TTCGAA 1 cut(s) 816
BspTI CTTAAG 1 cut(s) 614
BsrDI GCAATG 2 cut(s) 68, 492
BsrGI TGTACA 1 cut(s) 705
BsrI ACTGG 3 cut(s) 370, 545, 1025
BssECI CCNNGG 2 cut(s) 349, 851
BssMI GATC 5 cut(s) 165, 325, 439, 795, 1023
BssSI CACGAG 1 cut(s) 701
BssT1I CCWWGG 2 cut(s) 349, 851
Bst2BI CACGAG 1 cut(s) 701
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 2 cut(s) 49, 103
Bst6I CTCTTC 1 cut(s) 1055
BstAFI CTTAAG 1 cut(s) 614
BstAUI TGTACA 1 cut(s) 705
BstBAI YACGTR 2 cut(s) 413, 954
BstBI TTCGAA 1 cut(s) 816
BstC8I GCNNGC 2 cut(s) 307, 894
BstDEI CTNAG 2 cut(s) 531, 1136
BstDSI CCRYGG 1 cut(s) 851
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 3 cut(s) 242, 508, 943
BstKTI GATC 5 cut(s) 168, 328, 442, 798, 1026
BstMAI GTCTC 2 cut(s) 602, 1125
BstMBI GATC 5 cut(s) 165, 325, 439, 795, 1023
BstMWI GCNNNNNNNGC 1 cut(s) 503
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 2 cut(s) 180, 935
BstSNI TACGTA 2 cut(s) 413, 954
BstV1I GCAGC 4 cut(s) 43, 172, 433, 1080
BstX2I RGATCY 1 cut(s) 165
BstXI CCANNNNNNTGG 1 cut(s) 907
BstYI RGATCY 1 cut(s) 165
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 1 cut(s) 574
BtgI CCRYGG 1 cut(s) 851
BtgZI GCGATG 1 cut(s) 124
BtrI CACGTC 1 cut(s) 128
BtsCI GGATG 3 cut(s) 242, 508, 943
BtsIMutI CAGTG 1 cut(s) 1053
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 2 cut(s) 307, 894
Cfr13I GGNCC 3 cut(s) 92, 848, 1156
Csp6I GTAC 4 cut(s) 460, 706, 955, 1012
CviAII CATG 4 cut(s) 272, 563, 760, 852
CviQI GTAC 4 cut(s) 460, 706, 955, 1012
DdeI CTNAG 2 cut(s) 531, 1136
DpnI GATC 5 cut(s) 167, 327, 441, 797, 1025
DpnII GATC 5 cut(s) 165, 325, 439, 795, 1023
DriI GACNNNNNGTC 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 1055
Eam1105I GACNNNNNGTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 1055
Ecl136II GAGCTC 1 cut(s) 920
Eco105I TACGTA 2 cut(s) 413, 954
Eco130I CCWWGG 2 cut(s) 349, 851
Eco24I GRGCYC 1 cut(s) 922
Eco32I GATATC 1 cut(s) 235
Eco47I GGWCC 3 cut(s) 92, 848, 1156
Eco53kI GAGCTC 1 cut(s) 920
Eco57I CTGAAG 1 cut(s) 101
Eco88I CYCGRG 1 cut(s) 498
EcoICRI GAGCTC 1 cut(s) 920
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 971
EcoRII CCWGG 1 cut(s) 574
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 2 cut(s) 349, 851
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 2 cut(s) 349, 851
FaeI CATG 4 cut(s) 275, 566, 763, 855
FatI CATG 4 cut(s) 271, 562, 759, 851
FauNDI CATATG 3 cut(s) 263, 340, 787
Fnu4HI GCNGC 4 cut(s) 57, 186, 422, 1069
FokI GGATG 3 cut(s) 229, 515, 930
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 4 cut(s) 57, 186, 422, 1069
FspBI CTAG 1 cut(s) 893
GluI GCNGC 4 cut(s) 57, 186, 422, 1069
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 1 cut(s) 574
HapII CCGG 1 cut(s) 872
Hin1II CATG 4 cut(s) 275, 566, 763, 855
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 4 cut(s) 4, 354, 725, 1047
HpaII CCGG 1 cut(s) 872
Hpy166II GTNNAC 2 cut(s) 706, 877
Hpy188I TCNGA 3 cut(s) 120, 805, 848
Hpy188III TCNNGA 5 cut(s) 284, 437, 500, 530, 1085
Hpy8I GTNNAC 2 cut(s) 706, 877
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 203, 749, 850
HpyCH4III ACNGT 2 cut(s) 49, 103
HpyCH4IV ACGT 3 cut(s) 127, 412, 953
HpyCH4V TGCA 7 cut(s) 73, 188, 323, 791, 937, 943, 1169
HpyF10VI GCNNNNNNNGC 1 cut(s) 503
HpyF3I CTNAG 2 cut(s) 531, 1136
HpySE526I ACGT 3 cut(s) 127, 412, 953
Hsp92II CATG 4 cut(s) 275, 566, 763, 855
Kzo9I GATC 5 cut(s) 165, 325, 439, 795, 1023
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 4 cut(s) 43, 172, 433, 1080
LweI GCATC 4 cut(s) 5, 493, 796, 952
MaeI CTAG 1 cut(s) 893
MaeII ACGT 3 cut(s) 127, 412, 953
MaeIII GTNAC 1 cut(s) 754
MalI GATC 5 cut(s) 167, 327, 441, 797, 1025
MboI GATC 5 cut(s) 165, 325, 439, 795, 1023
MboII GAAGA 3 cut(s) 185, 218, 1042
MfeI CAATTG 2 cut(s) 201, 864
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 2 cut(s) 261, 922
MluCI AATT 6 cut(s) 201, 482, 587, 864, 971, 1143
MlyI GAGTC 2 cut(s) 719, 1041
MmeI TCCRAC 1 cut(s) 499
MseI TTAA 5 cut(s) 87, 393, 537, 551, 615
MspA1I CMGCKG 1 cut(s) 185
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 1 cut(s) 872
MspR9I CCNGG 1 cut(s) 576
MunI CAATTG 2 cut(s) 201, 864
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 1 cut(s) 503
NcoI CCATGG 1 cut(s) 851
NdeI CATATG 3 cut(s) 263, 340, 787
NdeII GATC 5 cut(s) 165, 325, 439, 795, 1023
NheI GCTAGC 1 cut(s) 892
NlaIII CATG 4 cut(s) 275, 566, 763, 855
NlaIV GGNNCC 2 cut(s) 94, 154
NspI RCATGY 1 cut(s) 566
NspV TTCGAA 1 cut(s) 816
PaqCI CACCTGC 1 cut(s) 928
PfeI GAWTC 2 cut(s) 4, 354
PflMI CCANNNNNTGG 1 cut(s) 654
PkrI GCNGC 4 cut(s) 58, 187, 423, 1070
PleI GAGTC 2 cut(s) 719, 1041
PpsI GAGTC 2 cut(s) 719, 1041
Ppu21I YACGTR 2 cut(s) 413, 954
PpuMI RGGWCCY 1 cut(s) 92
PsiI TTATAA 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 922
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 574
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 2 cut(s) 94, 154
PspPI GGNCC 3 cut(s) 92, 848, 1156
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 1 cut(s) 939
PsuI RGATCY 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 4 cut(s) 461, 707, 956, 1013
RsaNI GTAC 4 cut(s) 460, 706, 955, 1012
SacI GAGCTC 1 cut(s) 922
SaqAI TTAA 5 cut(s) 87, 393, 537, 551, 615
SatI GCNGC 4 cut(s) 57, 186, 422, 1069
Sau3AI GATC 5 cut(s) 165, 325, 439, 795, 1023
Sau96I GGNCC 3 cut(s) 92, 848, 1156
SchI GAGTC 2 cut(s) 719, 1041
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 2 cut(s) 261, 922
SfaNI GCATC 4 cut(s) 5, 493, 796, 952
SfcI CTRYAG 2 cut(s) 180, 935
SfuI TTCGAA 1 cut(s) 816
SinI GGWCC 3 cut(s) 92, 848, 1156
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SnaBI TACGTA 2 cut(s) 413, 954
Sse9I AATT 6 cut(s) 201, 482, 587, 864, 971, 1143
SspMI CTAG 1 cut(s) 893
SstI GAGCTC 1 cut(s) 922
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 2 cut(s) 349, 851
TaaI ACNGT 2 cut(s) 49, 103
TaiI ACGT 3 cut(s) 130, 415, 956
TaqI TCGA 2 cut(s) 285, 816
TasI AATT 6 cut(s) 201, 482, 587, 864, 971, 1143
TatI WGTACW 1 cut(s) 705
TfiI GAWTC 2 cut(s) 4, 354
Tru1I TTAA 5 cut(s) 87, 393, 537, 551, 615
Tru9I TTAA 5 cut(s) 87, 393, 537, 551, 615
TscAI CASTG 1 cut(s) 1060
TseI GCWGC 4 cut(s) 56, 185, 421, 1068
TspDTI ATGAA 3 cut(s) 211, 569, 978
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 1 cut(s) 1060
Van91I CCANNNNNTGG 1 cut(s) 654
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 3 cut(s) 92, 848, 1156
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 3 cut(s) 482, 587, 971
XceI RCATGY 1 cut(s) 566
XspI CTAG 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.