Rw7G004640

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007756
Physical Location & Seq
Reverse (-)
3867194 .. 3869841
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004640.1

Sequence Viewer

Length: 1179 bp
ATGGCTTCCTCACACTGCTTTGTTCCTTTTGTATTTCTACTACTCCTTGTTGGATGTTCCGCAAGGCCATTATATCCACTTCCAAGTAAAGAGGCCAATAGATATAGACAGCCTCTACAAACTTCTAGACCATACAACATTGCACATCGAGGATCAAATGGAGAGATCCCTGAAGAAACTGCTGCAGCATACATGAGAGCAATTGAAGAGGGTGCAGACTTCATTGAAACAGATATTCTATCTTCTAAAGATGGTGTCCTTATATGCTTTCATGATGTGACCCTCGATGAGACAACTGATATTGCAAAGCATACGGAATTTGCAGATCGCAAACGGACCTATGACGTTCAAGGGGTCAACACCACTGGTTTTTTCACTGTTGATTTTACACTTAAAGAACTGAAGACATTACGAGTGAAGCAGAGATACCCATTTCGTGATCAACAATATAATGGAAAATTTCCTATTATTACTTTTGAGGAGTTTATTTCAATCGCACTGGATGCACCCAGAGTTGTTGGCATATATCCGGAGATTAAGAATCCTGTATTGATCAACCAACATGTAAAGTGGTCAAATGGTAAGAGATTCGAGGACAAGTTTGTGGAGACACTTAAAAAGTACGGATACAAGGGTTCATATATGTCGAAAGATTGGCTGAAACAACCTGCATTTATCCAGTCATTTGCTCCAACTTCACTTGTTTATGTATCAAATCTGACAGACTTGCCCAAGATCTTTTTGATTGACGATATCACAATTCCTACTCAAGACACTAATCAGTCGTATTGGGAAATTACTTCAGACAGCTACCTTAGCTACATTAAGGAGTATGTGGTAGGCATTGGACCTTGGAAGGATACTGTGGTTCCTGTAGTAAATAATTATTTGCAAACACCTACAGACCTGGTCCCCATGGCTCATGCACATAACCTACAGGTGCACCCCTATACATTCAGGAATGAGAACTCCTTCCTGCACTTTGACTTTCATCAAGATCCGTATCAAGAGTACGAGTACTGGCTTAATAAGATAGGTGTTGATGGACTCTTTACAGACTTCACAGGCAGCCTGCATAATTATCAAGAATGGACCTCTTTTAATCAAAGTGATAACAGTAATGCATCAAAGCTATTGCATAAAATAGCATTGCTGGTCTCTTCCTATACAAAGGCATGA

Protein Analysis

392

Amino Acids

45.2

Weight (kDa)

5.85

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 49 - 356 2.2e-56 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AccIII TCCGGA 1 cut(s) 529
AciI CCGC 1 cut(s) 60
AclWI GGATC 3 cut(s) 160, 160, 992
AcsI RAATTY 2 cut(s) 317, 458
AcuI CTGAAG 3 cut(s) 192, 422, 786
AfaI GTAC 3 cut(s) 623, 1013, 1019
AflIII ACRYGT 1 cut(s) 562
AgsI TTSAA 4 cut(s) 206, 227, 350, 492
AjnI CCWGG 1 cut(s) 906
AluBI AGCT 3 cut(s) 810, 819, 1132
AluI AGCT 3 cut(s) 810, 819, 1132
Alw21I GWGCWC 1 cut(s) 945
Alw26I GTCTC 3 cut(s) 284, 602, 1162
Alw44I GTGCAC 1 cut(s) 941
AlwI GGATC 3 cut(s) 160, 160, 992
Aor13HI TCCGGA 1 cut(s) 529
AoxI GGCC 2 cut(s) 65, 93
ApaLI GTGCAC 1 cut(s) 941
ApeKI GCWGC 3 cut(s) 182, 185, 1068
ApoI RAATTY 2 cut(s) 317, 458
Asp700I GAANNNNTTC 1 cut(s) 971
AspS9I GGNCC 4 cut(s) 336, 848, 910, 1092
AvaII GGWCC 4 cut(s) 336, 848, 910, 1092
BaeGI GKGCMC 1 cut(s) 945
BaeI ACNNNNGTAYC 4 cut(s) 619, 652, 852, 885
BarI GAAGNNNNNNTAC 2 cut(s) 410, 442
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 945
BbvI GCAGC 3 cut(s) 169, 197, 1080
BccI CCATC 2 cut(s) 245, 1037
BciT130I CCWGG 1 cut(s) 908
BciVI GTATCC 2 cut(s) 620, 853
BclI TGATCA 2 cut(s) 439, 552
BcoDI GTCTC 3 cut(s) 284, 602, 1162
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 4 cut(s) 183, 873, 900, 935
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 2 cut(s) 620, 853
BglII AGATCT 1 cut(s) 735
BisI GCNGC 3 cut(s) 183, 186, 1069
BlsI GCNGC 3 cut(s) 184, 187, 1070
BmcAI AGTACT 1 cut(s) 1019
Bme1390I CCNGG 1 cut(s) 908
Bme18I GGWCC 4 cut(s) 336, 848, 910, 1092
BmgT120I GGNCC 4 cut(s) 336, 848, 910, 1092
BmiI GGNNCC 2 cut(s) 870, 912
BmrFI CCNGG 1 cut(s) 908
BmsI GCATC 2 cut(s) 493, 1133
BpiI GAAGAC 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 815
BpuEI CTTGAG 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 1002
BsaI GGTCTC 1 cut(s) 1162
BsaJI CCNNGG 2 cut(s) 851, 915
BsaWI WCCGGW 1 cut(s) 529
Bse1I ACTGG 4 cut(s) 370, 504, 679, 1025
Bse3DI GCAATG 2 cut(s) 138, 1148
Bse8I GATNNNNATC 1 cut(s) 1002
BseAI TCCGGA 1 cut(s) 529
BseBI CCWGG 1 cut(s) 908
BseDI CCNNGG 2 cut(s) 851, 915
BseGI GGATG 2 cut(s) 59, 508
BseJI GATNNNNATC 1 cut(s) 1002
BseMI GCAATG 2 cut(s) 138, 1148
BseNI ACTGG 4 cut(s) 370, 504, 679, 1025
BseRI GAGGAG 1 cut(s) 494
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 3 cut(s) 169, 197, 1080
BsgI GTGCAG 2 cut(s) 234, 962
BshFI GGCC 2 cut(s) 67, 95
BsiHKAI GWGCWC 1 cut(s) 945
BsiSI CCGG 1 cut(s) 530
BslFI GGGAC 1 cut(s) 896
BsmAI GTCTC 3 cut(s) 284, 602, 1162
BsmFI GGGAC 1 cut(s) 896
BsnI GGCC 2 cut(s) 67, 95
Bso31I GGTCTC 1 cut(s) 1162
Bsp1286I GDGCHC 1 cut(s) 945
Bsp13I TCCGGA 1 cut(s) 529
Bsp143I GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
Bsp19I CCATGG 1 cut(s) 915
BspACI CCGC 1 cut(s) 60
BspANI GGCC 2 cut(s) 67, 95
BspEI TCCGGA 1 cut(s) 529
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 2 cut(s) 870, 912
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 160, 160, 992
BspTNI GGTCTC 1 cut(s) 1162
BsrDI GCAATG 2 cut(s) 138, 1148
BsrI ACTGG 4 cut(s) 370, 504, 679, 1025
BssECI CCNNGG 2 cut(s) 851, 915
BssMI GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
BssT1I CCWWGG 2 cut(s) 851, 915
Bst2UI CCWGG 1 cut(s) 908
Bst4CI ACNGT 3 cut(s) 379, 865, 1118
Bst6I CTCTTC 2 cut(s) 201, 1165
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 1073
BstDEI CTNAG 1 cut(s) 815
BstDSI CCRYGG 1 cut(s) 915
BstF5I GGATG 2 cut(s) 59, 508
BstKTI GATC 7 cut(s) 155, 168, 328, 442, 555, 738, 1000
BstMAI GTCTC 3 cut(s) 284, 602, 1162
BstMBI GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
BstMWI GCNNNNNNNGC 2 cut(s) 503, 816
BstNI CCWGG 1 cut(s) 908
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 906
BstSFI CTRYAG 4 cut(s) 183, 873, 900, 935
BstSLI GKGCMC 1 cut(s) 945
BstV1I GCAGC 3 cut(s) 169, 197, 1080
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 3 cut(s) 165, 735, 997
BstYI RGATCY 3 cut(s) 165, 735, 997
BsuI GTATCC 2 cut(s) 620, 853
BsuRI GGCC 2 cut(s) 67, 95
BtgI CCRYGG 1 cut(s) 915
BtsCI GGATG 2 cut(s) 59, 508
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 4 cut(s) 13, 363, 375, 497
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 1 cut(s) 1073
CciI TCATGA 1 cut(s) 271
Cfr13I GGNCC 4 cut(s) 336, 848, 910, 1092
CsiI ACCWGGT 1 cut(s) 906
Csp6I GTAC 3 cut(s) 622, 1012, 1018
CviAII CATG 6 cut(s) 193, 272, 563, 916, 923, 1176
CviQI GTAC 3 cut(s) 622, 1012, 1018
DdeI CTNAG 1 cut(s) 815
DpnI GATC 7 cut(s) 154, 167, 327, 441, 554, 737, 999
DpnII GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
Eam1104I CTCTTC 2 cut(s) 201, 1165
EarI CTCTTC 2 cut(s) 201, 1165
Eco130I CCWWGG 2 cut(s) 851, 915
Eco31I GGTCTC 1 cut(s) 1162
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 4 cut(s) 336, 848, 910, 1092
Eco57I CTGAAG 3 cut(s) 192, 422, 786
EcoRII CCWGG 1 cut(s) 906
EcoRV GATATC 1 cut(s) 754
EcoT14I CCWWGG 2 cut(s) 851, 915
EcoT22I ATGCAT 1 cut(s) 1126
ErhI CCWWGG 2 cut(s) 851, 915
FaeI CATG 6 cut(s) 196, 275, 566, 919, 926, 1179
FaqI GGGAC 1 cut(s) 896
FatI CATG 6 cut(s) 192, 271, 562, 915, 922, 1175
FbaI TGATCA 2 cut(s) 439, 552
Fnu4HI GCNGC 3 cut(s) 183, 186, 1069
FokI GGATG 2 cut(s) 66, 515
Fsp4HI GCNGC 3 cut(s) 183, 186, 1069
FspBI CTAG 1 cut(s) 126
GluI GCNGC 3 cut(s) 183, 186, 1069
HaeIII GGCC 2 cut(s) 67, 95
HapII CCGG 1 cut(s) 530
Hin1II CATG 6 cut(s) 196, 275, 566, 919, 926, 1179
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HinfI GANTC 3 cut(s) 541, 588, 1047
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 2 cut(s) 358, 943
Hpy188I TCNGA 2 cut(s) 720, 805
Hpy188III TCNNGA 9 cut(s) 126, 272, 437, 530, 770, 958, 995, 1007, 1085
Hpy8I GTNNAC 2 cut(s) 358, 943
HpyAV CCTTC 2 cut(s) 850, 982
HpyCH4III ACNGT 3 cut(s) 379, 865, 1118
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 503, 816
HpyF3I CTNAG 1 cut(s) 815
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 6 cut(s) 196, 275, 566, 919, 926, 1179
Kpn2I TCCGGA 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 439, 552
Kzo9I GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 3 cut(s) 169, 197, 1080
LweI GCATC 2 cut(s) 493, 1133
MabI ACCWGGT 1 cut(s) 906
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 277
MalI GATC 7 cut(s) 154, 167, 327, 441, 554, 737, 999
MboI GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
MboII GAAGA 5 cut(s) 185, 218, 234, 415, 1152
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 3 cut(s) 165, 735, 997
MhlI GDGCHC 1 cut(s) 945
MluCI AATT 7 cut(s) 201, 317, 458, 759, 795, 883, 1078
MlyI GAGTC 1 cut(s) 1041
MmeI TCCRAC 2 cut(s) 31, 716
MnlI CCTC 9 cut(s) 19, 85, 123, 143, 202, 293, 472, 586, 1105
Mph1103I ATGCAT 1 cut(s) 1126
MroI TCCGGA 1 cut(s) 529
MroXI GAANNNNTTC 1 cut(s) 971
MseI TTAA 6 cut(s) 393, 537, 615, 825, 1026, 1101
MspI CCGG 1 cut(s) 530
MspR9I CCNGG 1 cut(s) 908
MunI CAATTG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 908
MwoI GCNNNNNNNGC 2 cut(s) 503, 816
NcoI CCATGG 1 cut(s) 915
NdeII GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
NlaIII CATG 6 cut(s) 196, 275, 566, 919, 926, 1179
NlaIV GGNNCC 2 cut(s) 870, 912
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 1126
NspI RCATGY 1 cut(s) 566
PagI TCATGA 1 cut(s) 271
PciI ACATGT 1 cut(s) 562
PdmI GAANNNNTTC 1 cut(s) 971
PfeI GAWTC 2 cut(s) 541, 588
PflFI GACNNNGTC 1 cut(s) 908
PkrI GCNGC 3 cut(s) 184, 187, 1070
PleI GAGTC 1 cut(s) 1041
PpsI GAGTC 1 cut(s) 1041
PscI ACATGT 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 906
PspGI CCWGG 1 cut(s) 906
PspN4I GGNNCC 2 cut(s) 870, 912
PspPI GGNCC 4 cut(s) 336, 848, 910, 1092
PstI CTGCAG 1 cut(s) 187
PsuI RGATCY 3 cut(s) 165, 735, 997
PsyI GACNNNGTC 1 cut(s) 908
RsaI GTAC 3 cut(s) 623, 1013, 1019
RsaNI GTAC 3 cut(s) 622, 1012, 1018
SaqAI TTAA 6 cut(s) 393, 537, 615, 825, 1026, 1101
SatI GCNGC 3 cut(s) 183, 186, 1069
Sau3AI GATC 7 cut(s) 152, 165, 325, 439, 552, 735, 997
Sau96I GGNCC 4 cut(s) 336, 848, 910, 1092
ScaI AGTACT 1 cut(s) 1019
SchI GAGTC 1 cut(s) 1041
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 945
SexAI ACCWGGT 1 cut(s) 906
SfaNI GCATC 2 cut(s) 493, 1133
SfcI CTRYAG 4 cut(s) 183, 873, 900, 935
SinI GGWCC 4 cut(s) 336, 848, 910, 1092
SmlI CTYRAG 1 cut(s) 768
SmoI CTYRAG 1 cut(s) 768
Sse9I AATT 7 cut(s) 201, 317, 458, 759, 795, 883, 1078
SsiI CCGC 1 cut(s) 60
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 906
StyI CCWWGG 2 cut(s) 851, 915
TaaI ACNGT 3 cut(s) 379, 865, 1118
TaiI ACGT 1 cut(s) 348
TaqI TCGA 4 cut(s) 148, 285, 591, 647
TasI AATT 7 cut(s) 201, 317, 458, 759, 795, 883, 1078
TatI WGTACW 1 cut(s) 1017
TfiI GAWTC 2 cut(s) 541, 588
Tru1I TTAA 6 cut(s) 393, 537, 615, 825, 1026, 1101
Tru9I TTAA 6 cut(s) 393, 537, 615, 825, 1026, 1101
TscAI CASTG 4 cut(s) 20, 370, 382, 504
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 3 cut(s) 182, 185, 1068
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 211, 260, 627, 980
TspGWI ACGGA 4 cut(s) 329, 349, 639, 990
TspRI CASTG 4 cut(s) 20, 370, 382, 504
Tth111I GACNNNGTC 1 cut(s) 908
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 4 cut(s) 336, 848, 910, 1092
XapI RAATTY 2 cut(s) 317, 458
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 971
XspI CTAG 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 1019
Zsp2I ATGCAT 1 cut(s) 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.