Rorug06G0454600

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002373
Physical Location & Seq
Reverse (-)
58275479 .. 58279175
3697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0454600.1

Sequence Viewer

Length: 1236 bp
ATGGAGCCACAAGAAGGCGATTCTGTATTCGCACACATTCCTCACGTTCCTGAAGTCCCAATTTACGCGATGATGGTTGCTTTCAGCAGAGACCCAAGTCCACTGAAGCTCAATTTAGGGATTGGTGTCTATAGAACTGAGGATGGGAAACCTTTTCAATTGAATGTAGTTAGACGAGTGGAGCGGTTACTAGTTAATGACAGGTCCACTTTTAAGGAGTATCTTCCTATTACTGGGACTGCTGAGTTCAACAGATTGAGTGCTAAGCTCATTTTGGGTGCTGACAGCCCTGCTATCAAGGAGAATAGAGTCACTACTGTCCAGTGCTTATCTGGTAGTGGATCACTGAGGGTCGGAGCTGAGTTTCTAGCAAACCATTACCACCATCAGCATACGGTCTACATGTCCCAGCCAACATATGCAGCCCACCCGGCTTTTTTCTCTGCAGCAGGGCTAGCTATCAAGACTTACCGTTACTATGACCCAGGAACATGTGGACTGGACTTCCAAGGAATGTTAGAAGATCTTTTGTCTGCCCCATCAGGAGCTATTGTGCTTCTCCAAGCATGTGCACATAACCCTACAGGTGTTGATCCAACTCTTCCACAGTGGGAGCAGATAAGACAGTTGATTAGAGCAAAGGGATTGTTTCCTTTCTTTGACTCTGCTTATCAGGGTCTTGTCAGTGGAAATCTGGATGAGGATGCACAATTTATTCGCCTGTTTGTTGCTGATGGTGGTGAGTGCCTTATAGCTCAGTCATATTCGAAGATTATGGGACTTTATGGAGAACGTGTTGGTGCCCTCAGCAGTGTTTGCAAGACAGCAGAAGTGGCTAGCCATGTTGAAAGCCAGCTGAAACTTGTGATCAGGCCCATGTATTCTAGCCCCCCAATTCATGGGGCATCCATAGTGGCCACTATCTTAAAGGATAGGGTTATGTATGAGGAATGGAGTATTGAAGTGAAGGCAATGACTGACCACCTTATTAGCACGCGTAGACAACTATTTGATGCTCTGCGTGATAGAGGTACTCCTGGTGACTGGGGGCACATTATCAGGCATGTTGGATTGTTTACTTTCTCAGGACTGAACCCTGAACAAGTTGCTTTCATGAATAAAGAGTACCATATCTACATGCCATCCGATGGGAGGATCAACATGGCAGCTCTAGGTCCTAAGACGGTTCCTCGTCTTGCAGATGCAATACATGCAGCTGTTAACATTGGTGTATAA

Protein Analysis

411

Amino Acids

45.33

Weight (kDa)

6.43

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 36 - 403 5.4e-84 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 2 cut(s) 899, 1148
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 2 cut(s) 399, 1000
AccII CGCG 2 cut(s) 68, 997
AciI CCGC 1 cut(s) 184
AclWI GGATC 3 cut(s) 349, 587, 1163
AcoI YGGCCR 1 cut(s) 915
AcuI CTGAAG 2 cut(s) 72, 125
AfaI GTAC 2 cut(s) 1033, 1127
AfiI CCNNNNNNNGG 5 cut(s) 14, 233, 899, 1148, 1152
AflIII ACRYGT 4 cut(s) 402, 491, 793, 995
AgsI TTSAA 5 cut(s) 158, 163, 250, 848, 962
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 2 cut(s) 484, 1036
AluBI AGCT 9 cut(s) 109, 268, 359, 458, 548, 755, 856, 1169, 1217
AluI AGCT 9 cut(s) 109, 268, 359, 458, 548, 755, 856, 1169, 1217
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 1 cut(s) 84
Alw44I GTGCAC 1 cut(s) 570
AlwI GGATC 3 cut(s) 349, 587, 1163
AoxI GGCC 2 cut(s) 872, 915
ApaLI GTGCAC 1 cut(s) 570
ApeKI GCWGC 4 cut(s) 422, 446, 1166, 1214
AspS9I GGNCC 3 cut(s) 204, 873, 1175
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 2 cut(s) 752, 1052
AsuII TTCGAA 1 cut(s) 767
AsuNHI GCTAGC 2 cut(s) 454, 836
AvaII GGWCC 2 cut(s) 204, 1175
BaeGI GKGCMC 3 cut(s) 574, 805, 1053
BalI TGGCCA 1 cut(s) 917
BanI GGYRCC 1 cut(s) 800
Bbv12I GWGCWC 1 cut(s) 574
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 4 cut(s) 434, 458, 1178, 1226
BccI CCATC 7 cut(s) 67, 137, 393, 547, 728, 1142, 1150
BciT130I CCWGG 2 cut(s) 486, 1038
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 84
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 6 cut(s) 191, 368, 455, 837, 885, 1172
BfmI CTRYAG 3 cut(s) 130, 444, 582
BglI GCCNNNNNGGC 1 cut(s) 431
BglII AGATCT 1 cut(s) 523
BisI GCNGC 4 cut(s) 423, 447, 1167, 1215
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 4 cut(s) 424, 448, 1168, 1216
Bme1390I CCNGG 3 cut(s) 431, 486, 1038
Bme18I GGWCC 2 cut(s) 204, 1175
BmgT120I GGNCC 3 cut(s) 204, 873, 1175
BmiI GGNNCC 3 cut(s) 6, 802, 1188
BmrFI CCNGG 3 cut(s) 431, 486, 1038
BmrI ACTGGG 2 cut(s) 243, 1054
BmsI GCATC 4 cut(s) 694, 914, 1003, 1192
BmtI GCTAGC 2 cut(s) 458, 840
BmuI ACTGGG 2 cut(s) 243, 1054
BoxI GACNNNNGTC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 806
Bpu1102I GCTNAGC 1 cut(s) 264
Bpu14I TTCGAA 1 cut(s) 767
BpuMI CCSGG 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 484, 508
BsaXI ACNNNNNCTCC 4 cut(s) 537, 567, 780, 810
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 233, 899, 1148, 1152
Bse1I ACTGG 4 cut(s) 238, 322, 504, 1049
Bse3DI GCAATG 1 cut(s) 978
BseBI CCWGG 2 cut(s) 486, 1038
BseDI CCNNGG 2 cut(s) 484, 508
BseGI GGATG 5 cut(s) 148, 703, 709, 905, 1142
BseLI CCNNNNNNNGG 5 cut(s) 14, 233, 899, 1148, 1152
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 7 cut(s) 129, 234, 338, 351, 770, 820, 1098
BseNI ACTGG 4 cut(s) 238, 322, 504, 1049
BseSI GKGCMC 3 cut(s) 574, 805, 1053
BseXI GCAGC 4 cut(s) 434, 458, 1178, 1226
BseYI CCCAGC 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 68, 997
BshFI GGCC 2 cut(s) 874, 917
BshNI GGYRCC 1 cut(s) 800
BsiHKAI GWGCWC 1 cut(s) 574
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 4 cut(s) 41, 250, 391, 792
BslI CCNNNNNNNGG 5 cut(s) 14, 233, 899, 1148, 1152
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 4 cut(s) 41, 250, 391, 792
BsnI GGCC 2 cut(s) 874, 917
Bso31I GGTCTC 1 cut(s) 84
Bsp119I TTCGAA 1 cut(s) 767
Bsp1286I GDGCHC 3 cut(s) 574, 805, 1053
Bsp143I GATC 5 cut(s) 341, 523, 592, 867, 1155
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 1 cut(s) 184
BspANI GGCC 2 cut(s) 874, 917
BspCNI CTCAG 7 cut(s) 130, 235, 339, 352, 769, 819, 1097
BspFNI CGCG 2 cut(s) 68, 997
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 3 cut(s) 6, 802, 1188
BspMAI CTGCAG 1 cut(s) 448
BspOI GCTAGC 2 cut(s) 458, 840
BspPI GGATC 3 cut(s) 349, 587, 1163
BspT104I TTCGAA 1 cut(s) 767
BspT107I GGYRCC 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 84
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 978
BsrI ACTGG 4 cut(s) 238, 322, 504, 1049
BssECI CCNNGG 2 cut(s) 484, 508
BssMI GATC 5 cut(s) 341, 523, 592, 867, 1155
BssT1I CCWWGG 1 cut(s) 508
Bst2UI CCWGG 2 cut(s) 486, 1038
Bst4CI ACNGT 6 cut(s) 319, 397, 473, 609, 627, 1186
Bst6I CTCTTC 1 cut(s) 606
BstAPI GCANNNNNTGC 2 cut(s) 816, 1211
BstBI TTCGAA 1 cut(s) 767
BstC8I GCNNGC 4 cut(s) 456, 838, 854, 995
BstDEI CTNAG 9 cut(s) 138, 243, 264, 347, 360, 756, 806, 1084, 1179
BstF5I GGATG 5 cut(s) 148, 703, 709, 905, 1142
BstFNI CGCG 2 cut(s) 68, 997
BstKTI GATC 5 cut(s) 344, 526, 595, 870, 1158
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 5 cut(s) 341, 523, 592, 867, 1155
BstMWI GCNNNNNNNGC 5 cut(s) 431, 455, 816, 833, 1211
BstNI CCWGG 2 cut(s) 486, 1038
BstNSI RCATGY 6 cut(s) 406, 495, 570, 1067, 1141, 1214
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 3 cut(s) 429, 484, 1036
BstSFI CTRYAG 3 cut(s) 130, 444, 582
BstSLI GKGCMC 3 cut(s) 574, 805, 1053
BstUI CGCG 2 cut(s) 68, 997
BstV1I GCAGC 4 cut(s) 434, 458, 1178, 1226
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 2 cut(s) 874, 917
BtgZI GCGATG 1 cut(s) 83
BtsCI GGATG 5 cut(s) 148, 703, 709, 905, 1142
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 6 cut(s) 101, 329, 344, 614, 691, 817
Cac8I GCNNGC 4 cut(s) 456, 838, 854, 995
CciI TCATGA 1 cut(s) 1113
Cfr13I GGNCC 3 cut(s) 204, 873, 1175
Csp6I GTAC 2 cut(s) 1032, 1126
CviQI GTAC 2 cut(s) 1032, 1126
DdeI CTNAG 9 cut(s) 138, 243, 264, 347, 360, 756, 806, 1084, 1179
DpnI GATC 5 cut(s) 343, 525, 594, 869, 1157
DpnII GATC 5 cut(s) 341, 523, 592, 867, 1155
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 84
Eco47I GGWCC 2 cut(s) 204, 1175
Eco57I CTGAAG 2 cut(s) 72, 125
EcoO109I RGGNCCY 1 cut(s) 1175
EcoRII CCWGG 2 cut(s) 484, 1036
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
FaqI GGGAC 4 cut(s) 41, 250, 391, 792
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 2 cut(s) 399, 1000
Fnu4HI GCNGC 4 cut(s) 423, 447, 1167, 1215
FokI GGATG 5 cut(s) 155, 710, 716, 892, 1129
Fsp4HI GCNGC 4 cut(s) 423, 447, 1167, 1215
FspBI CTAG 6 cut(s) 191, 368, 455, 837, 885, 1172
GluI GCNGC 4 cut(s) 423, 447, 1167, 1215
GsaI CCCAGC 1 cut(s) 412
HaeIII GGCC 2 cut(s) 874, 917
HapII CCGG 1 cut(s) 431
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HinfI GANTC 3 cut(s) 20, 309, 662
HpaI GTTAAC 1 cut(s) 1222
HpaII CCGG 1 cut(s) 431
HphI GGTGA 2 cut(s) 752, 1052
Hpy166II GTNNAC 8 cut(s) 101, 207, 400, 497, 572, 1001, 1077, 1222
Hpy188I TCNGA 2 cut(s) 356, 1147
Hpy188III TCNNGA 6 cut(s) 50, 463, 543, 695, 1086, 1114
Hpy8I GTNNAC 8 cut(s) 101, 207, 400, 497, 572, 1001, 1077, 1222
HpyAV CCTTC 2 cut(s) 8, 961
HpyCH4III ACNGT 6 cut(s) 319, 397, 473, 609, 627, 1186
HpyCH4IV ACGT 2 cut(s) 45, 793
HpyCH4V TGCA 8 cut(s) 422, 446, 572, 707, 819, 1199, 1205, 1214
HpyF10VI GCNNNNNNNGC 5 cut(s) 431, 455, 816, 833, 1211
HpyF3I CTNAG 9 cut(s) 138, 243, 264, 347, 360, 756, 806, 1084, 1179
HpySE526I ACGT 2 cut(s) 45, 793
Ksp22I TGATCA 1 cut(s) 867
KspAI GTTAAC 1 cut(s) 1222
Kzo9I GATC 5 cut(s) 341, 523, 592, 867, 1155
LmnI GCTCC 5 cut(s) 4, 181, 356, 545, 613
Lsp1109I GCAGC 4 cut(s) 434, 458, 1178, 1226
LweI GCATC 4 cut(s) 694, 914, 1003, 1192
MaeI CTAG 6 cut(s) 191, 368, 455, 837, 885, 1172
MaeII ACGT 2 cut(s) 45, 793
MaeIII GTNAC 4 cut(s) 186, 310, 473, 1040
MalI GATC 5 cut(s) 343, 525, 594, 869, 1157
MbiI CCGCTC 1 cut(s) 184
MboI GATC 5 cut(s) 341, 523, 592, 867, 1155
MboII GAAGA 4 cut(s) 215, 533, 593, 781
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 3 cut(s) 574, 805, 1053
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 5 cut(s) 60, 112, 158, 710, 894
MluI ACGCGT 1 cut(s) 995
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 2 cut(s) 318, 656
MmeI TCCRAC 3 cut(s) 334, 620, 1048
MnlI CCTC 9 cut(s) 51, 133, 342, 694, 815, 940, 1022, 1146, 1200
Mox20I TGGCCA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 917
MseI TTAA 4 cut(s) 195, 213, 926, 1221
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 2 cut(s) 856, 1217
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 3 cut(s) 431, 486, 1038
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 2 cut(s) 486, 1038
MvnI CGCG 2 cut(s) 68, 997
MwoI GCNNNNNNNGC 5 cut(s) 431, 455, 816, 833, 1211
NciI CCSGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 418
NdeII GATC 5 cut(s) 341, 523, 592, 867, 1155
NheI GCTAGC 2 cut(s) 454, 836
NlaIV GGNNCC 3 cut(s) 6, 802, 1188
NmuCI GTSAC 2 cut(s) 310, 1040
NspI RCATGY 6 cut(s) 406, 495, 570, 1067, 1141, 1214
NspV TTCGAA 1 cut(s) 767
PagI TCATGA 1 cut(s) 1113
PciI ACATGT 2 cut(s) 402, 491
PfeI GAWTC 1 cut(s) 20
PflMI CCANNNNNTGG 2 cut(s) 899, 1148
PkrI GCNGC 4 cut(s) 424, 448, 1168, 1216
PleI GAGTC 2 cut(s) 317, 656
PpsI GAGTC 2 cut(s) 317, 656
PpuMI RGGWCCY 1 cut(s) 1175
PscI ACATGT 2 cut(s) 402, 491
PshAI GACNNNNGTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 1175
Psp6I CCWGG 2 cut(s) 484, 1036
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 2 cut(s) 484, 1036
PspN4I GGNNCC 3 cut(s) 6, 802, 1188
PspPI GGNCC 3 cut(s) 204, 873, 1175
PspPPI RGGWCCY 1 cut(s) 1175
PstI CTGCAG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 523
PvuII CAGCTG 2 cut(s) 856, 1217
RsaI GTAC 2 cut(s) 1033, 1127
RsaNI GTAC 2 cut(s) 1032, 1126
SaqAI TTAA 4 cut(s) 195, 213, 926, 1221
SatI GCNGC 4 cut(s) 423, 447, 1167, 1215
Sau3AI GATC 5 cut(s) 341, 523, 592, 867, 1155
Sau96I GGNCC 3 cut(s) 204, 873, 1175
SchI GAGTC 2 cut(s) 318, 656
ScrFI CCNGG 3 cut(s) 431, 486, 1038
SduI GDGCHC 3 cut(s) 574, 805, 1053
SfaNI GCATC 4 cut(s) 694, 914, 1003, 1192
SfcI CTRYAG 3 cut(s) 130, 444, 582
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 2 cut(s) 204, 1175
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 5 cut(s) 60, 112, 158, 710, 894
SsiI CCGC 1 cut(s) 184
SspMI CTAG 6 cut(s) 191, 368, 455, 837, 885, 1172
StyD4I CCNGG 3 cut(s) 429, 484, 1036
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 6 cut(s) 319, 397, 473, 609, 627, 1186
TaiI ACGT 2 cut(s) 48, 796
TaqI TCGA 1 cut(s) 767
TasI AATT 5 cut(s) 60, 112, 158, 710, 894
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 4 cut(s) 195, 213, 926, 1221
Tru9I TTAA 4 cut(s) 195, 213, 926, 1221
TscAI CASTG 6 cut(s) 108, 329, 351, 614, 691, 817
TseFI GTSAC 2 cut(s) 310, 1040
TseI GCWGC 4 cut(s) 422, 446, 1166, 1214
Tsp45I GTSAC 2 cut(s) 310, 1040
TspDTI ATGAA 3 cut(s) 887, 1102, 1130
TspRI CASTG 6 cut(s) 108, 329, 351, 614, 691, 817
Van91I CCANNNNNTGG 2 cut(s) 899, 1148
VneI GTGCAC 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 204, 1175
XceI RCATGY 6 cut(s) 406, 495, 570, 1067, 1141, 1214
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmiI GTMKAC 2 cut(s) 399, 1000
XspI CTAG 6 cut(s) 191, 368, 455, 837, 885, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.