Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002373

Physical Location & Seq

Genomic Coordinates Reverse (-)

4563531 .. 4566481

Gene Structure 2951 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1067200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGGCTCAAACAGAGGAAGGCGGGAACATAGCAACCCAGCAGGAGTTCCCAATGCAGGGGAGTAATTTTCAAGGCGATTTGTATGGGAAAATGGTTGCCGGAAACAGTTTTCCGGGAAAATTCGGGGAGCTGACCTCCGAGGATTCGGAGGAGATTGAGCTGAGCCTGGGCCTGTCGCTGAACGGTCGGTTCGGAGTGGACCCCGCGAGAGCCAAAATGGTGGCTCTGAAACGCTCGTCGTCAATCTCCGATTTCGGTTTTACCCCGGCAAGAGAAGAAGAGACATGTGGTGTGTCAGCCTCGTCGCGCGCGGTGCCTCTGATGAGGACTTGTTCGCTTCCGACGGAGACAGAGGAGGAGTGGAGGAAGAGGAAGGAGCTCCAGAGCTTGAGGAGAATGGAAGCGAAGAGGAAGAGGTCGGAGAAGCAGCTGAGGAACTTGAAGGTGCCGAGGGATCGGAGCCGGGAGAACGGCGGCGATGAGGAGAAGGCGGCGGAGGTAGCGAATGGGGTTCATAGGAAAGAGCAGTTTGTGAAGGTTGTGGATGAGTTTAGAGCAATGGGGATGCCCAATTGTCCTGCTCCGCCGATACCGGCCGCATCTCGAGGTAGTGGCTCAACTGGGATTTCGGAATCTGAGAGCCAATCTGCTGCTACGCCTCAAGGAGTGCACAAATGCACCGAAGCAAGAAGCCACGCCGAAGCTCAGTCTTTGCCAAAGACTGAGAAGGAACTGCTAGCCACACCAAGGATGATAACCGCTCAAAGGTCATGTCAGTTCATTGGAGTCCAAATGGAAATCAACTGTAATAAACCCCCAGTTCCAGAATCCGGCCCCAACAAAATCGTGAGGAACGTGTTGGAGAGCATGCCTTGTGTGTCTACGACAGGAGATGGCCCTGACGGGAAAAGAATAGAAGGGTTTCTTTACAGATACAAGAAGGGTGAGGAAGTTAGGATAGTATGCGTTTGCCATGGCAGCTTTCTCTCGCCGGCGGAGTTTGTGAAGCATGCAGGCGGTGGTGACGTGGAGCACCCGTTGAAGCATATAGTGGTGAGTCCTAGTCCGTTACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EAR Jas NINJA_B

Protein Analysis

359

Amino Acids

39.07

Weight (kDa)

7.57

Isoelectric Point (pI)

68.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EAR	PF07897	47 - 84	3.3e-17	Ethylene-responsive binding factor-associated repression
NLS_NINJA_AFP	PF16136	107 - 143	4e-21	Putative nuclear localisation signal in Ninja/AFP proteins
TDBD	PF16135	319 - 352	2.4e-08	Tify domain binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29575 AT3G29575 AT3G29575
fragaria_vesca	FvH4_5g13760 FvH4_6g46820 FvH4_6g46820
malus_domestica	MD09G1067200.v1.1 MD17G1060200.v1.1
prunus_persica	Prupe.5G205900_v2.0.a1 Prupe.5G205900_v2.0.a1 Prupe.5G205900_v2.0.a1 Prupe.5G205900_v2.0.a1
pyrus_communis	pycom06g18020 pycom111g05650 pycom13g23090 pycom17g05990
rosa_chinensis	RchiOBHm_Chr2g0165801 RchiOBHm_Chr7g0183181
rosa_laevigata	RLG00000005069 RLG00000021557
rosa_multiflora	Rmu_co8279591.1_g000001 Rmu_co8332333.1_g000001 Rmu_sc0001861.1_g000014 Rmu_sc0003227.1_g000009 Rmu_sc0006832.1_g000002 Rmu_sc0012318.1_g000002 Rmu_ssc0000111.1_g000018
rosa_roxburghii	Rroxscaffold_2G00085490 Rroxscaffold_3G00270680
rosa_rugosa	Rorug02G0517800 Rorug06G0454600
rosa_samantha	Rh2AG583500 Rh2BG595600 Rh2CG221700 Rh2CG566300 Rh2DG605400 Rh7AG057000 Rh7BG056700 Rh7CG057800 Rh7DG056600
rosa_wichuraiana	Rw2G048660 Rw7G004650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	313, 445
AccBSI	CCGCTC	1 cut(s)	761
AccI	GTMKAC	1 cut(s)	881
AccII	CGCG	4 cut(s)	206, 307, 309, 311
AclWI	GGATC	1 cut(s)	462
AcoI	YGGCCR	1 cut(s)	594
AcsI	RAATTY	1 cut(s)	119
AfiI	CCNNNNNNNGG	5 cut(s)	55, 56, 747, 765, 830
AflIII	ACRYGT	2 cut(s)	284, 855
AgsI	TTSAA	3 cut(s)	71, 442, 1042
AjiI	CACGTC	1 cut(s)	1027
AjnI	CCWGG	1 cut(s)	165
AluBI	AGCT	7 cut(s)	130, 160, 379, 387, 430, 704, 981
AluI	AGCT	7 cut(s)	130, 160, 379, 387, 430, 704, 981
Alw21I	GWGCWC	3 cut(s)	381, 672, 1035
Alw26I	GTCTC	2 cut(s)	275, 341
Alw44I	GTGCAC	1 cut(s)	668
AlwI	GGATC	1 cut(s)	462
Ama87I	CYCGRG	1 cut(s)	603
AoxI	GGCC	4 cut(s)	169, 594, 832, 895
ApaLI	GTGCAC	1 cut(s)	668
ApeKI	GCWGC	3 cut(s)	427, 650, 978
ApoI	RAATTY	1 cut(s)	119
ArsI	GACNNNNNNTTYG	2 cut(s)	757, 789
Asp700I	GAANNNNTTC	1 cut(s)	921
AspLEI	GCGC	2 cut(s)	309, 311
AspS9I	GGNCC	4 cut(s)	169, 199, 833, 896
AsuC2I	CCSGG	3 cut(s)	114, 266, 464
AsuHPI	GGTGA	3 cut(s)	956, 1034, 1066
AsuNHI	GCTAGC	1 cut(s)	736
AvaI	CYCGRG	1 cut(s)	603
AvaII	GGWCC	1 cut(s)	199
BaeGI	GKGCMC	1 cut(s)	672
BanI	GGYRCC	2 cut(s)	313, 445
BanII	GRGCYC	1 cut(s)	381
Bbv12I	GWGCWC	3 cut(s)	381, 672, 1035
BbvCI	CCTCAGC	1 cut(s)	431
BbvI	GCAGC	3 cut(s)	439, 637, 990
BccI	CCATC	1 cut(s)	887
BceAI	ACGGC	1 cut(s)	487
BciT130I	CCWGG	1 cut(s)	167
BcnI	CCSGG	3 cut(s)	114, 266, 464
BcoDI	GTCTC	2 cut(s)	275, 341
BfaI	CTAG	2 cut(s)	737, 1062
BfmI	CTRYAG	1 cut(s)	1073
BisI	GCNGC	6 cut(s)	428, 475, 492, 597, 651, 979
BlpI	GCTNAGC	1 cut(s)	161
BlsI	GCNGC	6 cut(s)	429, 476, 493, 598, 652, 980
Bme1390I	CCNGG	4 cut(s)	114, 167, 266, 464
Bme18I	GGWCC	1 cut(s)	199
BmeT110I	CYCGRG	1 cut(s)	603
BmgBI	CACGTC	1 cut(s)	1027
BmgT120I	GGNCC	4 cut(s)	169, 199, 833, 896
BmiI	GGNNCC	5 cut(s)	201, 315, 447, 461, 835
BmrFI	CCNGG	4 cut(s)	114, 167, 266, 464
BmrI	ACTGGG	2 cut(s)	630, 812
BmsI	GCATC	2 cut(s)	555, 608
BmtI	GCTAGC	1 cut(s)	740
BmuI	ACTGGG	2 cut(s)	630, 812
BplI	GAGNNNNNCTC	2 cut(s)	119, 151
BpmI	CTGGAG	1 cut(s)	365
Bpu10I	CCTNAGC	1 cut(s)	431
Bpu1102I	GCTNAGC	1 cut(s)	161
BpuEI	CTTGAG	2 cut(s)	409, 645
BpuMI	CCSGG	3 cut(s)	114, 266, 464
BsaJI	CCNNGG	6 cut(s)	138, 166, 264, 449, 746, 973
BsaXI	ACNNNNNCTCC	2 cut(s)	1022, 1052
Bsc4I	CCNNNNNNNGG	5 cut(s)	55, 56, 747, 765, 830
Bse118I	RCCGGY	2 cut(s)	592, 991
Bse1I	ACTGG	2 cut(s)	625, 818
Bse3DI	GCAATG	1 cut(s)	564
BseBI	CCWGG	1 cut(s)	167
BseDI	CCNNGG	6 cut(s)	138, 166, 264, 449, 746, 973
BseGI	GGATG	3 cut(s)	550, 570, 756
BseLI	CCNNNNNNNGG	5 cut(s)	55, 56, 747, 765, 830
BseMI	GCAATG	1 cut(s)	564
BseMII	CTCAG	5 cut(s)	152, 422, 627, 714, 719
BseNI	ACTGG	2 cut(s)	625, 818
BsePI	GCGCGC	1 cut(s)	307
BseRI	GAGGAG	5 cut(s)	164, 368, 371, 406, 497
BseSI	GKGCMC	1 cut(s)	672
BseX3I	CGGCCG	1 cut(s)	594
BseXI	GCAGC	3 cut(s)	439, 637, 990
BseYI	CCCAGC	1 cut(s)	36
Bsh1236I	CGCG	4 cut(s)	206, 307, 309, 311
Bsh1285I	CGRYCG	2 cut(s)	187, 597
BshFI	GGCC	4 cut(s)	171, 596, 834, 897
BshNI	GGYRCC	2 cut(s)	313, 445
BsiEI	CGRYCG	2 cut(s)	187, 597
BsiHKAI	GWGCWC	3 cut(s)	381, 672, 1035
BsiHKCI	CYCGRG	1 cut(s)	603
BsiSI	CCGG	7 cut(s)	99, 113, 266, 463, 593, 831, 992
BslI	CCNNNNNNNGG	5 cut(s)	55, 56, 747, 765, 830
BsmAI	GTCTC	2 cut(s)	275, 341
BsnI	GGCC	4 cut(s)	171, 596, 834, 897
BsoBI	CYCGRG	1 cut(s)	603
Bsp1286I	GDGCHC	3 cut(s)	381, 672, 1035
Bsp143I	GATC	1 cut(s)	454
Bsp1720I	GCTNAGC	1 cut(s)	161
Bsp19I	CCATGG	1 cut(s)	973
BspANI	GGCC	4 cut(s)	171, 596, 834, 897
BspCNI	CTCAG	5 cut(s)	153, 423, 628, 715, 718
BspFNI	CGCG	4 cut(s)	206, 307, 309, 311
BspLI	GGNNCC	5 cut(s)	201, 315, 447, 461, 835
BspOI	GCTAGC	1 cut(s)	740
BspPI	GGATC	1 cut(s)	462
BspT107I	GGYRCC	2 cut(s)	313, 445
BsrBI	CCGCTC	1 cut(s)	761
BsrDI	GCAATG	1 cut(s)	564
BsrFI	RCCGGY	2 cut(s)	592, 991
BsrI	ACTGG	2 cut(s)	625, 818
BssAI	RCCGGY	2 cut(s)	592, 991
BssECI	CCNNGG	6 cut(s)	138, 166, 264, 449, 746, 973
BssHII	GCGCGC	1 cut(s)	307
BssMI	GATC	1 cut(s)	454
BssT1I	CCWWGG	2 cut(s)	746, 973
Bst2UI	CCWGG	1 cut(s)	167
Bst4CI	ACNGT	4 cut(s)	107, 185, 806, 1074
Bst6I	CTCTTC	4 cut(s)	273, 362, 401, 407
BstC8I	GCNNGC	6 cut(s)	309, 738, 869, 993, 1011, 1015
BstDEI	CTNAG	5 cut(s)	161, 431, 636, 705, 723
BstDSI	CCRYGG	1 cut(s)	973
BstF5I	GGATG	3 cut(s)	550, 570, 756
BstFNI	CGCG	4 cut(s)	206, 307, 309, 311
BstHHI	GCGC	2 cut(s)	309, 311
BstKTI	GATC	1 cut(s)	457
BstMAI	GTCTC	2 cut(s)	275, 341
BstMBI	GATC	1 cut(s)	454
BstMCI	CGRYCG	2 cut(s)	187, 597
BstMWI	GCNNNNNNNGC	3 cut(s)	313, 500, 978
BstNI	CCWGG	1 cut(s)	167
BstNSI	RCATGY	3 cut(s)	288, 871, 1013
BstSCI	CCNGG	4 cut(s)	112, 165, 264, 462
BstSFI	CTRYAG	1 cut(s)	1073
BstSLI	GKGCMC	1 cut(s)	672
BstUI	CGCG	4 cut(s)	206, 307, 309, 311
BstV1I	GCAGC	3 cut(s)	439, 637, 990
BstXI	CCANNNNNNTGG	1 cut(s)	220
BstZI	CGGCCG	1 cut(s)	594
BsuRI	GGCC	4 cut(s)	171, 596, 834, 897
BtgI	CCRYGG	1 cut(s)	973
BtgZI	GCGATG	1 cut(s)	492
BtrI	CACGTC	1 cut(s)	1027
BtsCI	GGATG	3 cut(s)	550, 570, 756
Cac8I	GCNNGC	6 cut(s)	309, 738, 869, 993, 1011, 1015
CfoI	GCGC	2 cut(s)	309, 311
Cfr10I	RCCGGY	2 cut(s)	592, 991
Cfr13I	GGNCC	4 cut(s)	169, 199, 833, 896
CviAII	CATG	5 cut(s)	285, 771, 868, 974, 1010
DdeI	CTNAG	5 cut(s)	161, 431, 636, 705, 723
DpnI	GATC	1 cut(s)	456
DpnII	GATC	1 cut(s)	454
EaeI	YGGCCR	1 cut(s)	594
EagI	CGGCCG	1 cut(s)	594
Eam1104I	CTCTTC	4 cut(s)	273, 362, 401, 407
EarI	CTCTTC	4 cut(s)	273, 362, 401, 407
EciI	GGCGGA	3 cut(s)	509, 573, 1010
Ecl136II	GAGCTC	1 cut(s)	379
EclXI	CGGCCG	1 cut(s)	594
Eco130I	CCWWGG	2 cut(s)	746, 973
Eco24I	GRGCYC	1 cut(s)	381
Eco47I	GGWCC	1 cut(s)	199
Eco52I	CGGCCG	1 cut(s)	594
Eco53kI	GAGCTC	1 cut(s)	379
Eco88I	CYCGRG	1 cut(s)	603
EcoICRI	GAGCTC	1 cut(s)	379
EcoRII	CCWGG	1 cut(s)	165
EcoT14I	CCWWGG	2 cut(s)	746, 973
EcoT38I	GRGCYC	1 cut(s)	381
ErhI	CCWWGG	2 cut(s)	746, 973
FaeI	CATG	5 cut(s)	288, 774, 871, 977, 1013
FalI	AAGNNNNNCTT	2 cut(s)	909, 941
FatI	CATG	5 cut(s)	284, 770, 867, 973, 1009
FauI	CCCGC	2 cut(s)	14, 211
FblI	GTMKAC	1 cut(s)	881
Fnu4HI	GCNGC	6 cut(s)	428, 475, 492, 597, 651, 979
FokI	GGATG	3 cut(s)	557, 577, 763
FriOI	GRGCYC	1 cut(s)	381
Fsp4HI	GCNGC	6 cut(s)	428, 475, 492, 597, 651, 979
FspBI	CTAG	2 cut(s)	737, 1062
GlaI	GCGC	2 cut(s)	308, 310
GluI	GCNGC	6 cut(s)	428, 475, 492, 597, 651, 979
GsaI	CCCAGC	1 cut(s)	40
GsuI	CTGGAG	1 cut(s)	365
HaeIII	GGCC	4 cut(s)	171, 596, 834, 897
HapII	CCGG	7 cut(s)	99, 113, 266, 463, 593, 831, 992
HhaI	GCGC	2 cut(s)	309, 311
Hin1II	CATG	5 cut(s)	288, 774, 871, 977, 1013
Hin6I	GCGC	2 cut(s)	307, 309
HinP1I	GCGC	2 cut(s)	307, 309
HinfI	GANTC	5 cut(s)	143, 632, 786, 827, 1057
HpaII	CCGG	7 cut(s)	99, 113, 266, 463, 593, 831, 992
HphI	GGTGA	3 cut(s)	956, 1034, 1066
Hpy166II	GTNNAC	3 cut(s)	199, 670, 882
Hpy188III	TCNNGA	4 cut(s)	382, 603, 824, 847
Hpy8I	GTNNAC	3 cut(s)	199, 670, 882
Hpy99I	CGWCG	3 cut(s)	241, 307, 346
HpyAV	CCTTC	8 cut(s)	11, 367, 436, 481, 529, 721, 911, 934
HpyCH4III	ACNGT	4 cut(s)	107, 185, 806, 1074
HpyCH4IV	ACGT	2 cut(s)	855, 1026
HpyCH4V	TGCA	4 cut(s)	55, 670, 678, 1013
HpyF10VI	GCNNNNNNNGC	3 cut(s)	313, 500, 978
HpyF3I	CTNAG	5 cut(s)	161, 431, 636, 705, 723
HpySE526I	ACGT	2 cut(s)	855, 1026
Hsp92II	CATG	5 cut(s)	288, 774, 871, 977, 1013
HspAI	GCGC	2 cut(s)	307, 309
KroI	GCCGGC	1 cut(s)	991
KroNI	GCCGGC	1 cut(s)	993
Kzo9I	GATC	1 cut(s)	454
LmnI	GCTCC	6 cut(s)	127, 376, 384, 459, 586, 1030
Lsp1109I	GCAGC	3 cut(s)	439, 637, 990
LweI	GCATC	2 cut(s)	555, 608
MaeI	CTAG	2 cut(s)	737, 1062
MaeII	ACGT	2 cut(s)	855, 1026
MaeIII	GTNAC	2 cut(s)	1022, 1068
MalI	GATC	1 cut(s)	456
MauBI	CGCGCGCG	1 cut(s)	307
MbiI	CCGCTC	1 cut(s)	761
MboI	GATC	1 cut(s)	454
MboII	GAAGA	5 cut(s)	287, 290, 379, 418, 424
MfeI	CAATTG	1 cut(s)	571
MhlI	GDGCHC	3 cut(s)	381, 672, 1035
MluCI	AATT	3 cut(s)	64, 119, 571
MlyI	GAGTC	2 cut(s)	795, 1066
MmeI	TCCRAC	3 cut(s)	365, 399, 840
MreI	CGCCGGCG	1 cut(s)	991
MroNI	GCCGGC	1 cut(s)	991
MroXI	GAANNNNTTC	1 cut(s)	921
MspA1I	CMGCKG	1 cut(s)	430
MspI	CCGG	7 cut(s)	99, 113, 266, 463, 593, 831, 992
MspR9I	CCNGG	4 cut(s)	114, 167, 266, 464
MunI	CAATTG	1 cut(s)	571
MvaI	CCWGG	1 cut(s)	167
MvnI	CGCG	4 cut(s)	206, 307, 309, 311
MwoI	GCNNNNNNNGC	3 cut(s)	313, 500, 978
NaeI	GCCGGC	1 cut(s)	993
NciI	CCSGG	3 cut(s)	114, 266, 464
NcoI	CCATGG	1 cut(s)	973
NdeII	GATC	1 cut(s)	454
NgoMIV	GCCGGC	1 cut(s)	991
NheI	GCTAGC	1 cut(s)	736
NlaIII	CATG	5 cut(s)	288, 774, 871, 977, 1013
NlaIV	GGNNCC	5 cut(s)	201, 315, 447, 461, 835
NmeAIII	GCCGAG	1 cut(s)	474
NmuCI	GTSAC	1 cut(s)	1022
NspI	RCATGY	3 cut(s)	288, 871, 1013
PaeI	GCATGC	2 cut(s)	871, 1013
PaeR7I	CTCGAG	1 cut(s)	603
PauI	GCGCGC	1 cut(s)	307
PciI	ACATGT	1 cut(s)	284
PcsI	WCGNNNNNNNCGW	1 cut(s)	852
PdiI	GCCGGC	1 cut(s)	993
PdmI	GAANNNNTTC	1 cut(s)	921
PfeI	GAWTC	3 cut(s)	143, 632, 827
PfoI	TCCNGGA	1 cut(s)	112
PkrI	GCNGC	6 cut(s)	429, 476, 493, 598, 652, 980
PleI	GAGTC	2 cut(s)	794, 1065
PpsI	GAGTC	2 cut(s)	794, 1065
PscI	ACATGT	1 cut(s)	284
Psp124BI	GAGCTC	1 cut(s)	381
Psp6I	CCWGG	1 cut(s)	165
PspFI	CCCAGC	1 cut(s)	36
PspGI	CCWGG	1 cut(s)	165
PspN4I	GGNNCC	5 cut(s)	201, 315, 447, 461, 835
PspPI	GGNCC	4 cut(s)	169, 199, 833, 896
PteI	GCGCGC	1 cut(s)	307
PvuII	CAGCTG	1 cut(s)	430
SacI	GAGCTC	1 cut(s)	381
SatI	GCNGC	6 cut(s)	428, 475, 492, 597, 651, 979
Sau3AI	GATC	1 cut(s)	454
Sau96I	GGNCC	4 cut(s)	169, 199, 833, 896
SchI	GAGTC	2 cut(s)	795, 1066
ScrFI	CCNGG	4 cut(s)	114, 167, 266, 464
SduI	GDGCHC	3 cut(s)	381, 672, 1035
SfaNI	GCATC	2 cut(s)	555, 608
SfcI	CTRYAG	1 cut(s)	1073
Sfr274I	CTCGAG	1 cut(s)	603
SgrAI	CRCCGGYG	1 cut(s)	991
SinI	GGWCC	1 cut(s)	199
SlaI	CTCGAG	1 cut(s)	603
SmlI	CTYRAG	3 cut(s)	388, 603, 660
SmoI	CTYRAG	3 cut(s)	388, 603, 660
SphI	GCATGC	2 cut(s)	871, 1013
Sse9I	AATT	3 cut(s)	64, 119, 571
SspMI	CTAG	2 cut(s)	737, 1062
SstI	GAGCTC	1 cut(s)	381
StyD4I	CCNGG	4 cut(s)	112, 165, 264, 462
StyI	CCWWGG	2 cut(s)	746, 973
TaaI	ACNGT	4 cut(s)	107, 185, 806, 1074
TaiI	ACGT	2 cut(s)	858, 1029
TaqI	TCGA	1 cut(s)	604
TasI	AATT	3 cut(s)	64, 119, 571
TauI	GCSGC	3 cut(s)	477, 494, 599
TfiI	GAWTC	3 cut(s)	143, 632, 827
TseFI	GTSAC	1 cut(s)	1022
TseI	GCWGC	3 cut(s)	427, 650, 978
Tsp45I	GTSAC	1 cut(s)	1022
TspDTI	ATGAA	2 cut(s)	503, 769
TspGWI	ACGGA	2 cut(s)	359, 1056
VneI	GTGCAC	1 cut(s)	668
VpaK11BI	GGWCC	1 cut(s)	199
XapI	RAATTY	1 cut(s)	119
XceI	RCATGY	3 cut(s)	288, 871, 1013
XhoI	CTCGAG	1 cut(s)	603
XmiI	GTMKAC	1 cut(s)	881
XmnI	GAANNNNTTC	1 cut(s)	921
XspI	CTAG	2 cut(s)	737, 1062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.