Rmu_ssc0000111.1_g000018

Ninja-family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000111.1
OG0002373
Physical Location & Seq
Reverse (-)
68255 .. 70767
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000111.1_g000018.1.cds

Sequence Viewer

Length: 795 bp
atggcgggcgaagaggaccaacacgatctcgggctctcacttgggggactttaccgaggcaactctggtggggcccgcatggcacgcgctgtgtcggtgggagtcaatgtgactgaggttgcggcggcgccgagtcccagggcggtttctctgccgtcggttagggagctggagatgatgagggtctctgaggttcagtttctgagcagggcgatggctacaagcctgaggttgactattcaggaggaaaaggctaagaaagctccggcagcggcggcccggctgcctgaagtggccgctctgggagtgaatattttggttgatgtggatctgaattgtgaaagcttctttggaaaattagatttggcttttggtgattttgaggaatccggatctggacgcttggctgcaacaagcaacacagttagttctgagcttaagtctggagaagggagctccaatcagcctgtggtcaccacgacaatgatggctaacacaaaaaaggtgaaggctgatgaccctgaacaagacagtgcagcaaagagggctagagtttatcatcgcaatgttgtgaggccaattaggcgtgaagggatggttagaatgccttctgtgaaaaccattggggctgatgggaagaaagtggaagggtacctgtacaagtatcttgaaggaaaagtgcggattgtatgtgcttgccatgggactttccagtccccagcggagttcgtcaagcatgccggtggcaatgaggtcgccaatcctttgaagcaaatcactgtttcccttgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
EAR Jas NINJA_B

Protein Analysis

264

Amino Acids

28.19

Weight (kDa)

8.7

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 712
Acc65I GGTACC 1 cut(s) 651
AccB1I GGYRCC 2 cut(s) 127, 651
AccBSI CCGCTC 1 cut(s) 299
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 389
AclWI GGATC 2 cut(s) 336, 400
AcoI YGGCCR 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 2 cut(s) 653, 659
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 2 cut(s) 671, 769
AjnI CCWGG 1 cut(s) 137
AjuI GAANNNNNNNTTGG 2 cut(s) 333, 365
AluBI AGCT 5 cut(s) 169, 263, 345, 436, 456
AluI AGCT 5 cut(s) 169, 263, 345, 436, 456
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 2 cut(s) 336, 400
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 29
Aor13HI TCCGGA 1 cut(s) 389
AoxI GGCC 4 cut(s) 72, 276, 294, 575
ApaI GGGCCC 1 cut(s) 76
ApeKI GCWGC 4 cut(s) 269, 283, 407, 536
Asp700I GAANNNNTTC 1 cut(s) 607
Asp718I GGTACC 1 cut(s) 651
AspLEI GCGC 2 cut(s) 89, 130
AspS9I GGNCC 4 cut(s) 16, 72, 73, 277
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 3 cut(s) 386, 466, 517
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 16
AxyI CCTNAGG 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 76
BanI GGYRCC 2 cut(s) 127, 651
BanII GRGCYC 3 cut(s) 36, 76, 458
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 4 cut(s) 270, 281, 394, 548
BccI CCATC 4 cut(s) 208, 481, 589, 626
BceAI ACGGC 1 cut(s) 139
BcgI CGANNNNNNTGC 2 cut(s) 736, 770
BciT130I CCWGG 1 cut(s) 139
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 190
BfaI CTAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 131
BfrI CTTAAG 1 cut(s) 437
BglI GCCNNNNNGGC 2 cut(s) 80, 583
BisI GCNGC 9 cut(s) 123, 126, 270, 273, 276, 284, 297, 408, 537
BlsI GCNGC 9 cut(s) 124, 127, 271, 274, 277, 285, 298, 409, 538
Bme1390I CCNGG 2 cut(s) 139, 280
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 4 cut(s) 16, 72, 73, 277
BmiI GGNNCC 4 cut(s) 73, 74, 129, 653
BmrFI CCNGG 2 cut(s) 139, 280
BpmI CTGGAG 2 cut(s) 191, 465
BpuMI CCSGG 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 190
BsaJI CCNNGG 4 cut(s) 55, 137, 138, 700
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 1 cut(s) 712
Bse21I CCTNAGG 1 cut(s) 227
Bse3DI GCAATG 2 cut(s) 571, 754
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 389
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 4 cut(s) 55, 137, 138, 700
BseGI GGATG 1 cut(s) 600
BseJI GATNNNNATC 1 cut(s) 327
BseMI GCAATG 2 cut(s) 571, 754
BseMII CTCAG 5 cut(s) 105, 180, 194, 218, 423
BseNI ACTGG 1 cut(s) 712
BseSI GKGCMC 1 cut(s) 76
BseXI GCAGC 4 cut(s) 270, 281, 394, 548
BseYI CCCAGC 1 cut(s) 718
BsgI GTGCAG 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 74, 278, 296, 577
BshNI GGYRCC 2 cut(s) 127, 651
BsiHKAI GWGCWC 1 cut(s) 458
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 4 cut(s) 266, 280, 390, 741
BslFI GGGAC 4 cut(s) 60, 120, 700, 718
BsmAI GTCTC 1 cut(s) 190
BsmFI GGGAC 4 cut(s) 60, 120, 700, 718
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 4 cut(s) 74, 278, 296, 577
Bso31I GGTCTC 1 cut(s) 190
BsoBI CYCGRG 1 cut(s) 29
Bsp120I GGGCCC 1 cut(s) 72
Bsp1286I GDGCHC 3 cut(s) 36, 76, 458
Bsp13I TCCGGA 1 cut(s) 389
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 3 cut(s) 25, 328, 392
Bsp19I CCATGG 1 cut(s) 700
BspANI GGCC 4 cut(s) 74, 278, 296, 577
BspCNI CTCAG 5 cut(s) 106, 181, 195, 219, 424
BspEI TCCGGA 1 cut(s) 389
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 4 cut(s) 73, 74, 129, 653
BspPI GGATC 2 cut(s) 336, 400
BspT107I GGYRCC 2 cut(s) 127, 651
BspTI CTTAAG 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 190
BsrBI CCGCTC 1 cut(s) 299
BsrDI GCAATG 2 cut(s) 571, 754
BsrFI RCCGGY 1 cut(s) 740
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 4 cut(s) 55, 137, 138, 700
BssMI GATC 3 cut(s) 25, 328, 392
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 700
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 3 cut(s) 424, 533, 781
Bst6I CTCTTC 1 cut(s) 6
BstACI GRCGYC 1 cut(s) 128
BstAFI CTTAAG 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 5 cut(s) 7, 76, 85, 697, 738
BstDEI CTNAG 6 cut(s) 114, 189, 203, 227, 255, 432
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 472
BstF5I GGATG 1 cut(s) 600
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 89, 130
BstKTI GATC 3 cut(s) 28, 331, 395
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 3 cut(s) 25, 328, 392
BstMWI GCNNNNNNNGC 7 cut(s) 80, 84, 260, 269, 275, 545, 583
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 740
BstPI GGTNACC 1 cut(s) 472
BstSCI CCNGG 2 cut(s) 137, 278
BstSLI GKGCMC 1 cut(s) 76
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 270, 281, 394, 548
BstX2I RGATCY 2 cut(s) 328, 392
BstYI RGATCY 2 cut(s) 328, 392
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 4 cut(s) 74, 278, 296, 577
BtgI CCRYGG 1 cut(s) 700
BtgZI GCGATG 2 cut(s) 227, 545
BtsCI GGATG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 538, 777
Cac8I GCNNGC 5 cut(s) 7, 76, 85, 697, 738
CaiI CAGNNNCTG 1 cut(s) 202
CfoI GCGC 2 cut(s) 89, 130
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 4 cut(s) 16, 72, 73, 277
CseI GACGC 1 cut(s) 408
Csp6I GTAC 2 cut(s) 652, 658
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 3 cut(s) 79, 701, 737
CviQI GTAC 2 cut(s) 652, 658
DdeI CTNAG 6 cut(s) 114, 189, 203, 227, 255, 432
DinI GGCGCC 1 cut(s) 129
DpnI GATC 3 cut(s) 27, 330, 394
DpnII GATC 3 cut(s) 25, 328, 392
DrdI GACNNNNNNGTC 1 cut(s) 712
DseDI GACNNNNNNGTC 1 cut(s) 712
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
Ecl136II GAGCTC 1 cut(s) 456
Eco130I CCWWGG 1 cut(s) 700
Eco24I GRGCYC 3 cut(s) 36, 76, 458
Eco31I GGTCTC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 309
Eco81I CCTNAGG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 472
EcoRII CCWGG 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 700
EcoT38I GRGCYC 3 cut(s) 36, 76, 458
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 700
FaeI CATG 3 cut(s) 82, 704, 740
FaiI YATR 4 cut(s) 80, 691, 702, 738
FaqI GGGAC 4 cut(s) 60, 120, 700, 718
FatI CATG 3 cut(s) 78, 700, 736
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 9 cut(s) 123, 126, 270, 273, 276, 284, 297, 408, 537
FokI GGATG 1 cut(s) 607
FriOI GRGCYC 3 cut(s) 36, 76, 458
Fsp4HI GCNGC 9 cut(s) 123, 126, 270, 273, 276, 284, 297, 408, 537
FspBI CTAG 1 cut(s) 549
GlaI GCGC 2 cut(s) 88, 129
GluI GCNGC 9 cut(s) 123, 126, 270, 273, 276, 284, 297, 408, 537
GsaI CCCAGC 1 cut(s) 722
GsuI CTGGAG 2 cut(s) 191, 465
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 4 cut(s) 74, 278, 296, 577
HapII CCGG 4 cut(s) 266, 280, 390, 741
HgaI GACGC 1 cut(s) 408
HhaI GCGC 2 cut(s) 89, 130
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 3 cut(s) 82, 704, 740
Hin6I GCGC 2 cut(s) 87, 128
HinP1I GCGC 2 cut(s) 87, 128
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 3 cut(s) 102, 133, 386
HpaII CCGG 4 cut(s) 266, 280, 390, 741
HphI GGTGA 3 cut(s) 386, 466, 517
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 4 cut(s) 190, 204, 333, 433
Hpy188III TCNNGA 5 cut(s) 242, 390, 396, 444, 668
Hpy8I GTNNAC 1 cut(s) 234
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 6 cut(s) 443, 502, 584, 618, 641, 665
HpyCH4III ACNGT 3 cut(s) 424, 533, 781
HpyCH4V TGCA 2 cut(s) 410, 536
HpyF10VI GCNNNNNNNGC 7 cut(s) 80, 84, 260, 269, 275, 545, 583
HpyF3I CTNAG 6 cut(s) 114, 189, 203, 227, 255, 432
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 3 cut(s) 82, 704, 740
HspAI GCGC 2 cut(s) 87, 128
KasI GGCGCC 1 cut(s) 127
Kpn2I TCCGGA 1 cut(s) 389
KpnI GGTACC 1 cut(s) 655
Kzo9I GATC 3 cut(s) 25, 328, 392
LmnI GCTCC 4 cut(s) 166, 268, 453, 461
Lsp1109I GCAGC 4 cut(s) 270, 281, 394, 548
MaeI CTAG 1 cut(s) 549
MaeIII GTNAC 2 cut(s) 109, 472
MalI GATC 3 cut(s) 27, 330, 394
MbiI CCGCTC 1 cut(s) 299
MboI GATC 3 cut(s) 25, 328, 392
MboII GAAGA 2 cut(s) 23, 649
MflI RGATCY 2 cut(s) 328, 392
MhlI GDGCHC 3 cut(s) 36, 76, 458
MluCI AATT 3 cut(s) 334, 356, 579
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 2 cut(s) 111, 142
MroI TCCGGA 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 607
MseI TTAA 2 cut(s) 438, 793
MslI CAYNNNNRTG 3 cut(s) 482, 564, 741
MspA1I CMGCKG 2 cut(s) 272, 722
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 4 cut(s) 266, 280, 390, 741
MspR9I CCNGG 2 cut(s) 139, 280
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 7 cut(s) 80, 84, 260, 269, 275, 545, 583
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 1 cut(s) 280
NcoI CCATGG 1 cut(s) 700
NdeII GATC 3 cut(s) 25, 328, 392
NlaIII CATG 3 cut(s) 82, 704, 740
NlaIV GGNNCC 4 cut(s) 73, 74, 129, 653
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 2 cut(s) 109, 472
NspI RCATGY 1 cut(s) 740
PaeI GCATGC 1 cut(s) 740
PasI CCCWGGG 1 cut(s) 138
PctI GAATGC 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 607
PfeI GAWTC 1 cut(s) 386
PkrI GCNGC 9 cut(s) 124, 127, 271, 274, 277, 285, 298, 409, 538
PleI GAGTC 2 cut(s) 110, 141
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 2 cut(s) 110, 141
Psp124BI GAGCTC 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 718
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 4 cut(s) 73, 74, 129, 653
PspOMI GGGCCC 1 cut(s) 72
PspPI GGNCC 4 cut(s) 16, 72, 73, 277
PstNI CAGNNNCTG 1 cut(s) 202
PsuI RGATCY 2 cut(s) 328, 392
RsaI GTAC 2 cut(s) 653, 659
RsaNI GTAC 2 cut(s) 652, 658
RseI CAYNNNNRTG 3 cut(s) 482, 564, 741
SacI GAGCTC 1 cut(s) 458
SaqAI TTAA 2 cut(s) 438, 793
SatI GCNGC 9 cut(s) 123, 126, 270, 273, 276, 284, 297, 408, 537
Sau3AI GATC 3 cut(s) 25, 328, 392
Sau96I GGNCC 4 cut(s) 16, 72, 73, 277
SchI GAGTC 2 cut(s) 111, 142
ScrFI CCNGG 2 cut(s) 139, 280
SduI GDGCHC 3 cut(s) 36, 76, 458
SfoI GGCGCC 1 cut(s) 129
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 3 cut(s) 482, 564, 741
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SphI GCATGC 1 cut(s) 740
Sse9I AATT 3 cut(s) 334, 356, 579
SspDI GGCGCC 1 cut(s) 127
SspI AATATT 1 cut(s) 313
SspMI CTAG 1 cut(s) 549
SstI GAGCTC 1 cut(s) 458
StyD4I CCNGG 2 cut(s) 137, 278
StyI CCWWGG 1 cut(s) 700
TaaI ACNGT 3 cut(s) 424, 533, 781
TasI AATT 3 cut(s) 334, 356, 579
TatI WGTACW 1 cut(s) 657
TauI GCSGC 5 cut(s) 125, 128, 275, 278, 299
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 2 cut(s) 438, 793
Tru9I TTAA 2 cut(s) 438, 793
TscAI CASTG 2 cut(s) 538, 784
TseFI GTSAC 2 cut(s) 109, 472
TseI GCWGC 4 cut(s) 269, 283, 407, 536
Tsp45I GTSAC 2 cut(s) 109, 472
TspRI CASTG 2 cut(s) 538, 784
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 16
XceI RCATGY 1 cut(s) 740
XcmI CCANNNNNNNNNTGG 2 cut(s) 466, 484
XmnI GAANNNNTTC 1 cut(s) 607
XspI CTAG 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.