Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0002373

Physical Location & Seq

Genomic Coordinates Forward (+)

81685695 .. 81689078

Gene Structure 3384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG583500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGGCCAAAACAGAGGAAGCTGGAAACAGAGCAACCCGGCAGCTTTTCTCATTGCCAGTGAGTAATTTTCAGGGAGATCTATTGAGGAGAATTGTTTCCGGCGACGATTTATCTCGAAAAGTCGTCGTTGACGAGCCGGTGCAAGATTCCGACGACGTTGAGCTGAGCCTGGGGCTGTCGTTGAACGGTCGGTTTGGGATGGACCCGAGAGCCAAGGCGGGGCTGCAACTCAAGCGATCGTCTTCAGTCTCCGACTTCTCGCCTGCCGCGAGCACTCCGGTGAGAGAGGAGGAAGCAGCGACGTGTCGTGTACCCCGGCCGTGTATGGTGCCTCTGATGAGGACTTGCTCGCTTCCGACGGAGACAGAGGATGAGTGGAGGAAGAGGAAGGAGCTGCAGAGCTTGAGGAGAATGGAGGCGAAGCGGAAGCGGTCGGAGAAGCAGCAGAGGAGTTATTCGAAGCTGCATAGGGATCGGAGCCGTGAGAATTTCGAGGAGGATAGGCGAGTGGAGGCCATCAATGGCGGCGGTTGTCCTCCCCTTCCGCCGCTACCGCCGGCGGAATCTCAGGGGACTATTGGGTCTCAGGGGAGTGGCTCCTCGGGGGTTACCGAGTCTGATGGCCAAGCTGTTCAAGTGGTGCCCAAATGCACCGAAGCGAGAAGCGATACCAATATTGAGTCATTGCCAAAGAGTGAAAAGGAACCACTCGTCACCCTGCAGATGAATTCTACTGTTGAAAACTCTGGGCAGTTGAACGGAGGGGTCCCAATGGAAAACCACTGCAACAAATCCACAAGTCCTGCCAAGGGTGCCAAGGAGATTGTGAGGAATGTGTTGGAAAATATGCCTTGTGTGTCTACTAAAGGAGATGGTCCAGACGGGAAAAGAATAGAAGGCTTTCTTTACAGGTACAAGAAAGGTGAGGAAGTGAGGATAGTATGCGTTTGTCATGGCAGCTTTCTGACTCCGGCTGAGTTTGTTAAGCATGCTGGTGGTGGTGATGTGCAACATCCTCTCAAACATATAGTGGTCAGTCCTGCTCCCATTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EAR Jas NINJA_B

Protein Analysis

351

Amino Acids

38.4

Weight (kDa)

8.32

Isoelectric Point (pI)

67.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EAR	PF07897	49 - 85	7.5e-16	Ethylene-responsive binding factor-associated repression
NLS_NINJA_AFP	PF16136	112 - 148	9.6e-21	Putative nuclear localisation signal in Ninja/AFP proteins
TDBD	PF16135	312 - 344	5.1e-08	Tify domain binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29575 AT3G29575 AT3G29575
fragaria_vesca	FvH4_5g13760 FvH4_6g46820 FvH4_6g46820
malus_domestica	MD09G1067200.v1.1 MD17G1060200.v1.1
prunus_persica	Prupe.5G205900_v2.0.a1 Prupe.5G205900_v2.0.a1 Prupe.5G205900_v2.0.a1 Prupe.5G205900_v2.0.a1
pyrus_communis	pycom06g18020 pycom111g05650 pycom13g23090 pycom17g05990
rosa_chinensis	RchiOBHm_Chr2g0165801 RchiOBHm_Chr7g0183181
rosa_laevigata	RLG00000005069 RLG00000021557
rosa_multiflora	Rmu_co8279591.1_g000001 Rmu_co8332333.1_g000001 Rmu_sc0001861.1_g000014 Rmu_sc0003227.1_g000009 Rmu_sc0006832.1_g000002 Rmu_sc0012318.1_g000002 Rmu_ssc0000111.1_g000018
rosa_roxburghii	Rroxscaffold_2G00085490 Rroxscaffold_3G00270680
rosa_rugosa	Rorug02G0517800 Rorug06G0454600
rosa_samantha	Rh2AG583500 Rh2BG595600 Rh2CG221700 Rh2CG566300 Rh2DG605400 Rh7AG057000 Rh7BG056700 Rh7CG057800 Rh7DG056600
rosa_wichuraiana	Rw2G048660 Rw7G004650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	580
AccB1I	GGYRCC	3 cut(s)	328, 640, 812
AccI	GTMKAC	1 cut(s)	860
AccII	CGCG	1 cut(s)	269
AclWI	GGATC	1 cut(s)	480
AcoI	YGGCCR	3 cut(s)	3, 317, 622
AcsI	RAATTY	2 cut(s)	487, 727
AcuI	CTGAAG	1 cut(s)	228
AfaI	GTAC	2 cut(s)	312, 914
AfiI	CCNNNNNNNGG	2 cut(s)	219, 809
AflIII	ACRYGT	1 cut(s)	302
AgsI	TTSAA	4 cut(s)	184, 635, 740, 757
AjiI	CACGTC	1 cut(s)	303
AjnI	CCWGG	1 cut(s)	168
AleI	CACNNNNGTG	1 cut(s)	278
AluBI	AGCT	8 cut(s)	20, 43, 163, 394, 402, 463, 629, 960
AluI	AGCT	8 cut(s)	20, 43, 163, 394, 402, 463, 629, 960
Alw21I	GWGCWC	1 cut(s)	275
Alw26I	GTCTC	3 cut(s)	253, 356, 588
AlwI	GGATC	1 cut(s)	480
Ama87I	CYCGRG	2 cut(s)	205, 601
AoxI	GGCC	4 cut(s)	3, 317, 513, 622
ApeKI	GCWGC	7 cut(s)	40, 223, 296, 394, 442, 463, 957
ApoI	RAATTY	2 cut(s)	487, 727
Asp700I	GAANNNNTTC	2 cut(s)	94, 900
AspS9I	GGNCC	3 cut(s)	202, 766, 875
AsuC2I	CCSGG	2 cut(s)	37, 316
AsuHPI	GGTGA	4 cut(s)	292, 706, 935, 1013
AsuII	TTCGAA	1 cut(s)	458
AvaI	CYCGRG	2 cut(s)	205, 601
AvaII	GGWCC	3 cut(s)	202, 766, 875
BaeGI	GKGCMC	1 cut(s)	645
BalI	TGGCCA	2 cut(s)	5, 624
BanI	GGYRCC	3 cut(s)	328, 640, 812
BbsI	GAAGAC	1 cut(s)	234
Bbv12I	GWGCWC	1 cut(s)	275
BbvI	GCAGC	7 cut(s)	52, 210, 308, 381, 450, 454, 969
BccI	CCATC	4 cut(s)	193, 524, 614, 866
BceAI	ACGGC	2 cut(s)	304, 465
BcgI	CGANNNNNNTGC	2 cut(s)	455, 489
BciT130I	CCWGG	1 cut(s)	170
BcnI	CCSGG	2 cut(s)	37, 316
BcoDI	GTCTC	3 cut(s)	253, 356, 588
BfmI	CTRYAG	2 cut(s)	395, 719
BglII	AGATCT	1 cut(s)	76
BlpI	GCTNAGC	1 cut(s)	164
Bme1390I	CCNGG	3 cut(s)	37, 170, 316
Bme18I	GGWCC	3 cut(s)	202, 766, 875
BmeT110I	CYCGRG	2 cut(s)	205, 601
BmgBI	CACGTC	1 cut(s)	303
BmgT120I	GGNCC	3 cut(s)	202, 766, 875
BmiI	GGNNCC	9 cut(s)	204, 330, 479, 598, 642, 705, 767, 768, 814
BmrFI	CCNGG	3 cut(s)	37, 170, 316
BpiI	GAAGAC	1 cut(s)	234
Bpu1102I	GCTNAGC	1 cut(s)	164
Bpu14I	TTCGAA	1 cut(s)	458
BpuEI	CTTGAG	2 cut(s)	215, 424
BpuMI	CCSGG	2 cut(s)	37, 316
BsaI	GGTCTC	1 cut(s)	588
BsaJI	CCNNGG	6 cut(s)	169, 213, 314, 600, 807, 816
BsaWI	WCCGGW	1 cut(s)	277
Bsc4I	CCNNNNNNNGG	2 cut(s)	219, 809
Bse118I	RCCGGY	2 cut(s)	136, 556
Bse1I	ACTGG	1 cut(s)	56
Bse3DI	GCAATG	2 cut(s)	50, 683
BseBI	CCWGG	1 cut(s)	170
BseDI	CCNNGG	6 cut(s)	169, 213, 314, 600, 807, 816
BseGI	GGATG	3 cut(s)	204, 376, 1012
BseLI	CCNNNNNNNGG	2 cut(s)	219, 809
BseMI	GCAATG	2 cut(s)	50, 683
BseMII	CTCAG	4 cut(s)	155, 581, 599, 966
BseNI	ACTGG	1 cut(s)	56
BseRI	GAGGAG	6 cut(s)	100, 302, 421, 463, 509, 589
BseSI	GKGCMC	1 cut(s)	645
BseX3I	CGGCCG	1 cut(s)	317
BseXI	GCAGC	7 cut(s)	52, 210, 308, 381, 450, 454, 969
Bsh1236I	CGCG	1 cut(s)	269
Bsh1285I	CGRYCG	4 cut(s)	190, 239, 320, 434
BshFI	GGCC	4 cut(s)	5, 319, 515, 624
BshNI	GGYRCC	3 cut(s)	328, 640, 812
BsiEI	CGRYCG	4 cut(s)	190, 239, 320, 434
BsiHKAI	GWGCWC	1 cut(s)	275
BsiHKCI	CYCGRG	2 cut(s)	205, 601
BsiSI	CCGG	7 cut(s)	37, 99, 137, 278, 316, 557, 971
BslFI	GGGAC	2 cut(s)	586, 752
BslI	CCNNNNNNNGG	2 cut(s)	219, 809
BsmAI	GTCTC	3 cut(s)	253, 356, 588
BsmFI	GGGAC	2 cut(s)	586, 752
BsnI	GGCC	4 cut(s)	5, 319, 515, 624
Bso31I	GGTCTC	1 cut(s)	588
BsoBI	CYCGRG	2 cut(s)	205, 601
Bsp119I	TTCGAA	1 cut(s)	458
Bsp1286I	GDGCHC	2 cut(s)	275, 645
Bsp143I	GATC	3 cut(s)	76, 236, 472
Bsp1720I	GCTNAGC	1 cut(s)	164
BspANI	GGCC	4 cut(s)	5, 319, 515, 624
BspCNI	CTCAG	4 cut(s)	156, 580, 598, 967
BspFNI	CGCG	1 cut(s)	269
BspLI	GGNNCC	9 cut(s)	204, 330, 479, 598, 642, 705, 767, 768, 814
BspMAI	CTGCAG	2 cut(s)	399, 723
BspPI	GGATC	1 cut(s)	480
BspT104I	TTCGAA	1 cut(s)	458
BspT107I	GGYRCC	3 cut(s)	328, 640, 812
BspTNI	GGTCTC	1 cut(s)	588
BsrDI	GCAATG	2 cut(s)	50, 683
BsrFI	RCCGGY	2 cut(s)	136, 556
BsrI	ACTGG	1 cut(s)	56
BssAI	RCCGGY	2 cut(s)	136, 556
BssECI	CCNNGG	6 cut(s)	169, 213, 314, 600, 807, 816
BssMI	GATC	3 cut(s)	76, 236, 472
BssT1I	CCWWGG	3 cut(s)	213, 807, 816
Bst2UI	CCWGG	1 cut(s)	170
Bst4CI	ACNGT	2 cut(s)	188, 736
Bst6I	CTCTTC	1 cut(s)	377
BstBI	TTCGAA	1 cut(s)	458
BstC8I	GCNNGC	5 cut(s)	264, 271, 350, 558, 990
BstDEI	CTNAG	4 cut(s)	164, 567, 585, 975
BstEII	GGTNACC	1 cut(s)	607
BstF5I	GGATG	3 cut(s)	204, 376, 1012
BstFNI	CGCG	1 cut(s)	269
BstKTI	GATC	3 cut(s)	79, 239, 475
BstMAI	GTCTC	3 cut(s)	253, 356, 588
BstMBI	GATC	3 cut(s)	76, 236, 472
BstMCI	CGRYCG	4 cut(s)	190, 239, 320, 434
BstMWI	GCNNNNNNNGC	3 cut(s)	232, 553, 812
BstNI	CCWGG	1 cut(s)	170
BstNSI	RCATGY	1 cut(s)	992
BstPI	GGTNACC	1 cut(s)	607
BstSCI	CCNGG	3 cut(s)	35, 168, 314
BstSFI	CTRYAG	2 cut(s)	395, 719
BstSLI	GKGCMC	1 cut(s)	645
BstUI	CGCG	1 cut(s)	269
BstV1I	GCAGC	7 cut(s)	52, 210, 308, 381, 450, 454, 969
BstV2I	GAAGAC	1 cut(s)	234
BstX2I	RGATCY	1 cut(s)	76
BstYI	RGATCY	1 cut(s)	76
BstZI	CGGCCG	1 cut(s)	317
BsuRI	GGCC	4 cut(s)	5, 319, 515, 624
BtrI	CACGTC	1 cut(s)	303
BtsCI	GGATG	3 cut(s)	204, 376, 1012
BtsI	GCAGTG	1 cut(s)	781
BtsIMutI	CAGTG	2 cut(s)	63, 781
Cac8I	GCNNGC	5 cut(s)	264, 271, 350, 558, 990
Cfr10I	RCCGGY	2 cut(s)	136, 556
Cfr13I	GGNCC	3 cut(s)	202, 766, 875
Csp6I	GTAC	2 cut(s)	311, 913
CviAII	CATG	2 cut(s)	953, 989
CviQI	GTAC	2 cut(s)	311, 913
DdeI	CTNAG	4 cut(s)	164, 567, 585, 975
DpnI	GATC	3 cut(s)	78, 238, 474
DpnII	GATC	3 cut(s)	76, 236, 472
DrdI	GACNNNNNNGTC	1 cut(s)	580
DseDI	GACNNNNNNGTC	1 cut(s)	580
EaeI	YGGCCR	3 cut(s)	3, 317, 622
EagI	CGGCCG	1 cut(s)	317
Eam1104I	CTCTTC	1 cut(s)	377
EarI	CTCTTC	1 cut(s)	377
EciI	GGCGGA	2 cut(s)	534, 575
EclXI	CGGCCG	1 cut(s)	317
Eco130I	CCWWGG	3 cut(s)	213, 807, 816
Eco31I	GGTCTC	1 cut(s)	588
Eco47I	GGWCC	3 cut(s)	202, 766, 875
Eco52I	CGGCCG	1 cut(s)	317
Eco57I	CTGAAG	1 cut(s)	228
Eco88I	CYCGRG	2 cut(s)	205, 601
Eco91I	GGTNACC	1 cut(s)	607
EcoO109I	RGGNCCY	1 cut(s)	766
EcoO65I	GGTNACC	1 cut(s)	607
EcoRI	GAATTC	1 cut(s)	727
EcoRII	CCWGG	1 cut(s)	168
EcoT14I	CCWWGG	3 cut(s)	213, 807, 816
ErhI	CCWWGG	3 cut(s)	213, 807, 816
FaeI	CATG	2 cut(s)	956, 992
FaiI	YATR	8 cut(s)	326, 468, 848, 943, 954, 990, 1026, 1028
FalI	AAGNNNNNCTT	2 cut(s)	888, 920
FaqI	GGGAC	2 cut(s)	586, 752
FatI	CATG	2 cut(s)	952, 988
FauI	CCCGC	1 cut(s)	211
FblI	GTMKAC	1 cut(s)	860
FokI	GGATG	3 cut(s)	211, 383, 999
HaeIII	GGCC	4 cut(s)	5, 319, 515, 624
HapII	CCGG	7 cut(s)	37, 99, 137, 278, 316, 557, 971
Hin1II	CATG	2 cut(s)	956, 992
HincII	GTYRAC	1 cut(s)	130
HindII	GTYRAC	1 cut(s)	130
HinfI	GANTC	5 cut(s)	146, 563, 614, 680, 967
HpaII	CCGG	7 cut(s)	37, 99, 137, 278, 316, 557, 971
HphI	GGTGA	4 cut(s)	292, 706, 935, 1013
Hpy166II	GTNNAC	3 cut(s)	130, 311, 861
Hpy188I	TCNGA	8 cut(s)	151, 253, 336, 357, 436, 477, 619, 966
Hpy188III	TCNNGA	2 cut(s)	114, 878
Hpy8I	GTNNAC	3 cut(s)	130, 311, 861
Hpy99I	CGWCG	6 cut(s)	107, 128, 155, 158, 304, 361
HpyAV	CCTTC	3 cut(s)	382, 551, 890
HpyCH4III	ACNGT	2 cut(s)	188, 736
HpyCH4IV	ACGT	2 cut(s)	156, 302
HpyCH4V	TGCA	8 cut(s)	142, 226, 397, 466, 651, 721, 786, 1009
HpyF10VI	GCNNNNNNNGC	3 cut(s)	232, 553, 812
HpyF3I	CTNAG	4 cut(s)	164, 567, 585, 975
HpySE526I	ACGT	2 cut(s)	156, 302
Hsp92II	CATG	2 cut(s)	956, 992
KflI	GGGWCCC	1 cut(s)	766
KroI	GCCGGC	1 cut(s)	556
KroNI	GCCGGC	1 cut(s)	558
Kzo9I	GATC	3 cut(s)	76, 236, 472
LmnI	GCTCC	4 cut(s)	391, 477, 602, 1048
Lsp1109I	GCAGC	7 cut(s)	52, 210, 308, 381, 450, 454, 969
MaeII	ACGT	2 cut(s)	156, 302
MaeIII	GTNAC	2 cut(s)	607, 712
MalI	GATC	3 cut(s)	78, 238, 474
MboI	GATC	3 cut(s)	76, 236, 472
MboII	GAAGA	2 cut(s)	234, 394
MflI	RGATCY	1 cut(s)	76
MhlI	GDGCHC	2 cut(s)	275, 645
MlsI	TGGCCA	2 cut(s)	5, 624
MluCI	AATT	4 cut(s)	64, 90, 487, 727
MluNI	TGGCCA	2 cut(s)	5, 624
MlyI	GAGTC	3 cut(s)	623, 689, 961
MmeI	TCCRAC	5 cut(s)	174, 276, 380, 414, 819
Mox20I	TGGCCA	2 cut(s)	5, 624
MreI	CGCCGGCG	1 cut(s)	556
MroNI	GCCGGC	1 cut(s)	556
MroXI	GAANNNNTTC	2 cut(s)	94, 900
MscI	TGGCCA	2 cut(s)	5, 624
MseI	TTAA	1 cut(s)	984
MslI	CAYNNNNRTG	2 cut(s)	278, 993
Msp20I	TGGCCA	2 cut(s)	5, 624
MspI	CCGG	7 cut(s)	37, 99, 137, 278, 316, 557, 971
MspR9I	CCNGG	3 cut(s)	37, 170, 316
MvaI	CCWGG	1 cut(s)	170
MvnI	CGCG	1 cut(s)	269
MwoI	GCNNNNNNNGC	3 cut(s)	232, 553, 812
NaeI	GCCGGC	1 cut(s)	558
NciI	CCSGG	2 cut(s)	37, 316
NdeII	GATC	3 cut(s)	76, 236, 472
NgoMIV	GCCGGC	1 cut(s)	556
NlaIII	CATG	2 cut(s)	956, 992
NlaIV	GGNNCC	9 cut(s)	204, 330, 479, 598, 642, 705, 767, 768, 814
NmuCI	GTSAC	1 cut(s)	712
NspI	RCATGY	1 cut(s)	992
NspV	TTCGAA	1 cut(s)	458
OliI	CACNNNNGTG	1 cut(s)	278
PaeI	GCATGC	1 cut(s)	992
PcsI	WCGNNNNNNNCGW	2 cut(s)	129, 266
PdiI	GCCGGC	1 cut(s)	558
PdmI	GAANNNNTTC	2 cut(s)	94, 900
PfeI	GAWTC	2 cut(s)	146, 563
Ple19I	CGATCG	1 cut(s)	239
PleI	GAGTC	3 cut(s)	622, 688, 961
PpsI	GAGTC	3 cut(s)	622, 688, 961
PpuMI	RGGWCCY	1 cut(s)	766
Psp5II	RGGWCCY	1 cut(s)	766
Psp6I	CCWGG	1 cut(s)	168
PspEI	GGTNACC	1 cut(s)	607
PspGI	CCWGG	1 cut(s)	168
PspN4I	GGNNCC	9 cut(s)	204, 330, 479, 598, 642, 705, 767, 768, 814
PspPI	GGNCC	3 cut(s)	202, 766, 875
PspPPI	RGGWCCY	1 cut(s)	766
PstI	CTGCAG	2 cut(s)	399, 723
PsuI	RGATCY	1 cut(s)	76
PvuI	CGATCG	1 cut(s)	239
RsaI	GTAC	2 cut(s)	312, 914
RsaNI	GTAC	2 cut(s)	311, 913
RseI	CAYNNNNRTG	2 cut(s)	278, 993
SaqAI	TTAA	1 cut(s)	984
Sau3AI	GATC	3 cut(s)	76, 236, 472
Sau96I	GGNCC	3 cut(s)	202, 766, 875
SchI	GAGTC	3 cut(s)	623, 689, 961
ScrFI	CCNGG	3 cut(s)	37, 170, 316
SduI	GDGCHC	2 cut(s)	275, 645
SfcI	CTRYAG	2 cut(s)	395, 719
SfuI	TTCGAA	1 cut(s)	458
SgrAI	CRCCGGYG	1 cut(s)	556
SinI	GGWCC	3 cut(s)	202, 766, 875
SmiMI	CAYNNNNRTG	2 cut(s)	278, 993
SmlI	CTYRAG	2 cut(s)	230, 403
SmoI	CTYRAG	2 cut(s)	230, 403
SphI	GCATGC	1 cut(s)	992
Sse9I	AATT	4 cut(s)	64, 90, 487, 727
SspI	AATATT	1 cut(s)	676
StyD4I	CCNGG	3 cut(s)	35, 168, 314
StyI	CCWWGG	3 cut(s)	213, 807, 816
TaaI	ACNGT	2 cut(s)	188, 736
TaiI	ACGT	2 cut(s)	159, 305
TaqI	TCGA	3 cut(s)	115, 458, 492
TasI	AATT	4 cut(s)	64, 90, 487, 727
TauI	GCSGC	3 cut(s)	269, 528, 550
TfiI	GAWTC	2 cut(s)	146, 563
Tru1I	TTAA	1 cut(s)	984
Tru9I	TTAA	1 cut(s)	984
TscAI	CASTG	2 cut(s)	63, 788
TseFI	GTSAC	1 cut(s)	712
TseI	GCWGC	7 cut(s)	40, 223, 296, 394, 442, 463, 957
Tsp45I	GTSAC	1 cut(s)	712
TspDTI	ATGAA	1 cut(s)	740
TspGWI	ACGGA	2 cut(s)	374, 774
TspRI	CASTG	2 cut(s)	63, 788
VpaK11BI	GGWCC	3 cut(s)	202, 766, 875
XapI	RAATTY	2 cut(s)	487, 727
XceI	RCATGY	1 cut(s)	992
XmiI	GTMKAC	1 cut(s)	860
XmnI	GAANNNNTTC	2 cut(s)	94, 900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.