RLG00000005069

Ninja-family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002373
Physical Location & Seq
Forward (+)
64817989 .. 64820532
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005069

Sequence Viewer

Length: 723 bp
ATGGCGGGCGAAGAGGACCAACACGATCTCGGGCTCTCACTTGGGGGACTTTACCAAGGCAACTCGGGTGGGTCCCGCGTGGCACGCGCTGCGTCGGTGGGAGTCGATGTGACTGAGGTGCCGGCGGCGCCGCGTCCCAGGGCTGTTTCTCTGCCGTCGGTTAGGGAGCTGGAGATGATGAGGGTCTCTGAGGTTCAGTTTCTAAGCAGGGCGATGGCTGCAAGCCTGAGGTTGAATATTCGGGAGGAAAAGACTAAGAAAGCTCCGGCAGCGGCTGCCCGAGTGCCTGAAGTGGCTGCACTGGGAGTGATTGTGACTGGATCTGGACGCTTGGCTGCAACAAGCAGCACAGTCAGTTCTGAGCTTATGTCTGGAGAAGGGAGCCCCAATCAGCCTGTGGTCACCGTGTCAATGGTGGCTAACACAAAAAAGGTGAAGGCTGATGACCCTGAACAGGACAGTGCAGCAAAGAGGGCTAGAGTTTATCATTGCAATGTTGTGAGGCCAATTAGGCGTGAAGGGATGGTTAGAATGCCTTCTGTGAAAACCATTGGGGCTGATGGGAAGAAAGTGGAAGGGTACCTGTACAAGTATCTTGAAGGAAAAGTGCGGATTGTGTGTGCTTGCCATGGGACTTTCCAGTCCCCAGCGGAGTTCGTCAAGCATGCCGGTGGAAATGAGGTCGCCAATCCTTTGAAGCAAATCACTGTTTCCCTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
EAR Jas NINJA_B

Protein Analysis

241

Amino Acids

25.47

Weight (kDa)

9.49

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 204 - 236 2.3e-07 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 640
Acc65I GGTACC 1 cut(s) 579
AccB1I GGYRCC 3 cut(s) 118, 127, 579
AccII CGCG 3 cut(s) 78, 87, 133
AciI CCGC 7 cut(s) 5, 76, 125, 131, 272, 610, 650
AclWI GGATC 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 2 cut(s) 581, 587
AfiI CCNNNNNNNGG 1 cut(s) 454
AgsI TTSAA 3 cut(s) 235, 599, 697
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 3 cut(s) 169, 263, 364
AluI AGCT 3 cut(s) 169, 263, 364
Alw26I GTCTC 1 cut(s) 190
AlwI GGATC 1 cut(s) 328
AlwNI CAGNNNCTG 1 cut(s) 275
Ama87I CYCGRG 3 cut(s) 29, 64, 279
AoxI GGCC 1 cut(s) 503
ApeKI GCWGC 8 cut(s) 89, 218, 269, 275, 296, 335, 345, 464
Asp700I GAANNNNTTC 1 cut(s) 535
Asp718I GGTACC 1 cut(s) 579
AspLEI GCGC 2 cut(s) 89, 130
AspS9I GGNCC 2 cut(s) 16, 72
AsuHPI GGTGA 2 cut(s) 394, 445
AvaI CYCGRG 3 cut(s) 29, 64, 279
AvaII GGWCC 2 cut(s) 16, 72
AxyI CCTNAGG 1 cut(s) 227
BanI GGYRCC 3 cut(s) 118, 127, 579
BanII GRGCYC 2 cut(s) 36, 386
BbvI GCAGC 8 cut(s) 76, 205, 262, 281, 283, 322, 357, 476
BccI CCATC 3 cut(s) 208, 517, 554
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 190
BfaI CTAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 131
BglI GCCNNNNNGGC 1 cut(s) 511
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 2 cut(s) 16, 72
BmeT110I CYCGRG 3 cut(s) 29, 64, 279
BmgT120I GGNCC 2 cut(s) 16, 72
BmiI GGNNCC 6 cut(s) 73, 74, 120, 129, 383, 581
BmrFI CCNGG 1 cut(s) 139
BmrI ACTGGG 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 311
BpmI CTGGAG 2 cut(s) 191, 393
BsaHI GRCGYC 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 190
BsaJI CCNNGG 4 cut(s) 55, 137, 138, 628
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 297, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 454
Bse118I RCCGGY 2 cut(s) 121, 668
Bse1I ACTGG 3 cut(s) 306, 322, 640
Bse21I CCTNAGG 1 cut(s) 227
Bse3DI GCAATG 2 cut(s) 487, 499
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 4 cut(s) 55, 137, 138, 628
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 1 cut(s) 454
BseMI GCAATG 2 cut(s) 487, 499
BseMII CTCAG 4 cut(s) 105, 180, 218, 351
BseNI ACTGG 3 cut(s) 306, 322, 640
BseXI GCAGC 8 cut(s) 76, 205, 262, 281, 283, 322, 357, 476
BseYI CCCAGC 1 cut(s) 646
BsgI GTGCAG 2 cut(s) 282, 483
Bsh1236I CGCG 3 cut(s) 78, 87, 133
BshFI GGCC 1 cut(s) 505
BshNI GGYRCC 3 cut(s) 118, 127, 579
BsiHKCI CYCGRG 3 cut(s) 29, 64, 279
BsiSI CCGG 3 cut(s) 122, 266, 669
BslFI GGGAC 5 cut(s) 58, 60, 120, 628, 646
BslI CCNNNNNNNGG 1 cut(s) 454
BsmAI GTCTC 1 cut(s) 190
BsmFI GGGAC 5 cut(s) 58, 60, 120, 628, 646
BsmI GAATGC 1 cut(s) 537
BsnI GGCC 1 cut(s) 505
Bso31I GGTCTC 1 cut(s) 190
BsoBI CYCGRG 3 cut(s) 29, 64, 279
Bsp1286I GDGCHC 2 cut(s) 36, 386
Bsp1407I TGTACA 1 cut(s) 585
Bsp143I GATC 2 cut(s) 25, 320
Bsp19I CCATGG 1 cut(s) 628
BspACI CCGC 7 cut(s) 5, 76, 125, 131, 272, 610, 650
BspANI GGCC 1 cut(s) 505
BspCNI CTCAG 4 cut(s) 106, 181, 219, 352
BspFNI CGCG 3 cut(s) 78, 87, 133
BspLI GGNNCC 6 cut(s) 73, 74, 120, 129, 383, 581
BspPI GGATC 1 cut(s) 328
BspT107I GGYRCC 3 cut(s) 118, 127, 579
BspTNI GGTCTC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 487, 499
BsrFI RCCGGY 2 cut(s) 121, 668
BsrGI TGTACA 1 cut(s) 585
BsrI ACTGG 3 cut(s) 306, 322, 640
BssAI RCCGGY 2 cut(s) 121, 668
BssECI CCNNGG 4 cut(s) 55, 137, 138, 628
BssMI GATC 2 cut(s) 25, 320
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 2 cut(s) 55, 628
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 4 cut(s) 352, 406, 461, 709
Bst6I CTCTTC 1 cut(s) 6
BstACI GRCGYC 1 cut(s) 128
BstAPI GCANNNNNTGC 2 cut(s) 89, 275
BstAUI TGTACA 1 cut(s) 585
BstC8I GCNNGC 6 cut(s) 7, 85, 123, 223, 625, 666
BstDEI CTNAG 6 cut(s) 114, 189, 203, 227, 255, 360
BstDSI CCRYGG 1 cut(s) 628
BstEII GGTNACC 1 cut(s) 400
BstF5I GGATG 1 cut(s) 528
BstFNI CGCG 3 cut(s) 78, 87, 133
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 2 cut(s) 89, 130
BstKTI GATC 2 cut(s) 28, 323
BstMAI GTCTC 1 cut(s) 190
BstMBI GATC 2 cut(s) 25, 320
BstMWI GCNNNNNNNGC 8 cut(s) 84, 89, 127, 218, 269, 275, 473, 511
BstNI CCWGG 1 cut(s) 139
BstNSI RCATGY 1 cut(s) 668
BstPI GGTNACC 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 137
BstUI CGCG 3 cut(s) 78, 87, 133
BstV1I GCAGC 8 cut(s) 76, 205, 262, 281, 283, 322, 357, 476
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 1 cut(s) 505
BtgI CCRYGG 1 cut(s) 628
BtgZI GCGATG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 3 cut(s) 299, 466, 705
Cac8I GCNNGC 6 cut(s) 7, 85, 123, 223, 625, 666
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 2 cut(s) 89, 130
Cfr10I RCCGGY 2 cut(s) 121, 668
Cfr13I GGNCC 2 cut(s) 16, 72
CseI GACGC 3 cut(s) 81, 122, 336
Csp6I GTAC 2 cut(s) 580, 586
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 2 cut(s) 629, 665
CviQI GTAC 2 cut(s) 580, 586
DdeI CTNAG 6 cut(s) 114, 189, 203, 227, 255, 360
DinI GGCGCC 1 cut(s) 129
DpnI GATC 2 cut(s) 27, 322
DpnII GATC 2 cut(s) 25, 320
DrdI GACNNNNNNGTC 1 cut(s) 640
DseDI GACNNNNNNGTC 1 cut(s) 640
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 55, 628
Eco24I GRGCYC 2 cut(s) 36, 386
Eco31I GGTCTC 1 cut(s) 190
Eco47I GGWCC 2 cut(s) 16, 72
Eco57I CTGAAG 1 cut(s) 309
Eco81I CCTNAGG 1 cut(s) 227
Eco88I CYCGRG 3 cut(s) 29, 64, 279
Eco91I GGTNACC 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 137
EcoT14I CCWWGG 2 cut(s) 55, 628
EcoT38I GRGCYC 2 cut(s) 36, 386
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 2 cut(s) 55, 628
FaeI CATG 2 cut(s) 632, 668
FaiI YATR 3 cut(s) 368, 630, 666
FaqI GGGAC 5 cut(s) 58, 60, 120, 628, 646
FatI CATG 2 cut(s) 628, 664
FauI CCCGC 1 cut(s) 83
FokI GGATG 1 cut(s) 535
FriOI GRGCYC 2 cut(s) 36, 386
FspBI CTAG 1 cut(s) 477
GlaI GCGC 2 cut(s) 88, 129
GsaI CCCAGC 1 cut(s) 650
GsuI CTGGAG 2 cut(s) 191, 393
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 1 cut(s) 505
HapII CCGG 3 cut(s) 122, 266, 669
HgaI GACGC 3 cut(s) 81, 122, 336
HhaI GCGC 2 cut(s) 89, 130
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 2 cut(s) 632, 668
Hin6I GCGC 2 cut(s) 87, 128
HinP1I GCGC 2 cut(s) 87, 128
HinfI GANTC 1 cut(s) 102
HpaII CCGG 3 cut(s) 122, 266, 669
HphI GGTGA 2 cut(s) 394, 445
Hpy188I TCNGA 2 cut(s) 190, 361
Hpy188III TCNNGA 4 cut(s) 242, 324, 372, 596
Hpy99I CGWCG 2 cut(s) 97, 160
HpyAV CCTTC 6 cut(s) 371, 430, 512, 546, 569, 593
HpyCH4III ACNGT 4 cut(s) 352, 406, 461, 709
HpyCH4V TGCA 5 cut(s) 221, 299, 338, 464, 492
HpyF10VI GCNNNNNNNGC 8 cut(s) 84, 89, 127, 218, 269, 275, 473, 511
HpyF3I CTNAG 6 cut(s) 114, 189, 203, 227, 255, 360
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 2 cut(s) 632, 668
HspAI GCGC 2 cut(s) 87, 128
KasI GGCGCC 1 cut(s) 127
KflI GGGWCCC 1 cut(s) 72
KpnI GGTACC 1 cut(s) 583
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 2 cut(s) 25, 320
LmnI GCTCC 3 cut(s) 166, 268, 381
Lsp1109I GCAGC 8 cut(s) 76, 205, 262, 281, 283, 322, 357, 476
MaeI CTAG 1 cut(s) 477
MaeIII GTNAC 3 cut(s) 109, 313, 400
MalI GATC 2 cut(s) 27, 322
MboI GATC 2 cut(s) 25, 320
MboII GAAGA 2 cut(s) 23, 577
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 2 cut(s) 36, 386
MluCI AATT 1 cut(s) 507
Mly113I GGCGCC 1 cut(s) 128
MlyI GAGTC 1 cut(s) 111
MnlI CCTC 9 cut(s) 7, 109, 174, 184, 222, 238, 465, 495, 673
MroNI GCCGGC 1 cut(s) 121
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 1 cut(s) 721
MslI CAYNNNNRTG 2 cut(s) 492, 669
MspA1I CMGCKG 2 cut(s) 272, 650
MspI CCGG 3 cut(s) 122, 266, 669
MspR9I CCNGG 1 cut(s) 139
Mva1269I GAATGC 1 cut(s) 537
MvaI CCWGG 1 cut(s) 139
MvnI CGCG 3 cut(s) 78, 87, 133
MwoI GCNNNNNNNGC 8 cut(s) 84, 89, 127, 218, 269, 275, 473, 511
NaeI GCCGGC 1 cut(s) 123
NarI GGCGCC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 628
NdeII GATC 2 cut(s) 25, 320
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 632, 668
NlaIV GGNNCC 6 cut(s) 73, 74, 120, 129, 383, 581
NmuCI GTSAC 3 cut(s) 109, 313, 400
NspI RCATGY 1 cut(s) 668
PaeI GCATGC 1 cut(s) 668
PasI CCCWGGG 1 cut(s) 138
PctI GAATGC 1 cut(s) 537
PdiI GCCGGC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 535
PleI GAGTC 1 cut(s) 110
PluTI GGCGCC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 110
PpuMI RGGWCCY 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 137
PspEI GGTNACC 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 646
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 6 cut(s) 73, 74, 120, 129, 383, 581
PspPI GGNCC 2 cut(s) 16, 72
PspPPI RGGWCCY 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 2 cut(s) 581, 587
RsaNI GTAC 2 cut(s) 580, 586
RseI CAYNNNNRTG 2 cut(s) 492, 669
SaqAI TTAA 1 cut(s) 721
Sau3AI GATC 2 cut(s) 25, 320
Sau96I GGNCC 2 cut(s) 16, 72
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 2 cut(s) 36, 386
SetI ASST 9 cut(s) 120, 171, 195, 233, 265, 366, 435, 585, 684
SfoI GGCGCC 1 cut(s) 129
SinI GGWCC 2 cut(s) 16, 72
SmiMI CAYNNNNRTG 2 cut(s) 492, 669
SphI GCATGC 1 cut(s) 668
Sse9I AATT 1 cut(s) 507
SsiI CCGC 7 cut(s) 5, 76, 125, 131, 272, 610, 650
SspDI GGCGCC 1 cut(s) 127
SspI AATATT 1 cut(s) 238
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 137
StyI CCWWGG 2 cut(s) 55, 628
TaaI ACNGT 4 cut(s) 352, 406, 461, 709
TaqI TCGA 1 cut(s) 105
TasI AATT 1 cut(s) 507
TatI WGTACW 1 cut(s) 585
TauI GCSGC 3 cut(s) 128, 133, 275
Tru1I TTAA 1 cut(s) 721
Tru9I TTAA 1 cut(s) 721
TscAI CASTG 3 cut(s) 306, 466, 712
TseFI GTSAC 3 cut(s) 109, 313, 400
TseI GCWGC 8 cut(s) 89, 218, 269, 275, 296, 335, 345, 464
Tsp45I GTSAC 3 cut(s) 109, 313, 400
TspRI CASTG 3 cut(s) 306, 466, 712
VpaK11BI GGWCC 2 cut(s) 16, 72
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.