Rorug02G0517800

Ninja-family protein AFP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002373
Physical Location & Seq
Reverse (-)
64865956 .. 64872442
6487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0517800.1

Sequence Viewer

Length: 2673 bp
ATGGAGGATTTGCCTCCGCCACCTCCTATTGTACCACCGAATGTGAAGCCAGAAGTTGTGGCTCTGGCTCCTCCTAAGCACTCTATTATGGTTAGGCCACGTGGGAGTGGAACCACAGGGCGGCGCATACAATTGAGCACTAACCACTTCAAAGTTTCTGTGAAGAATCCAGATGCTGTGTTCTACCAGTACTCTGTTCTGATAACTTATGAAGATAAGAGACCTGTTGAAGGCATGGGACTTGGGAGAAAGCTGGTGGATAAGCTCTATCAAACATACACTTCAGAGTTTGCTGGTAAAAAGTTTGCATATGATGGGGAGAAAGCTTTATACACAGTCGGTCCTCTCCCGCGAAACAAACTTGAGTTCACAGTTGTTTTGGAGGAGTCATTTACAAAGAGCAACAAAATTGGGAAGTCTGGTGAGACTGATAAAAGGATGAAGCACTGTTCCAATCGGTCGAAAACATTCAATGTAGAGATAAGTTATGCTGCCAAAATACCTCTCAAGTCAATTGCTCTTGCCCTTCAAGGAGCTGAAGTAGACAACACTCAGGATGCATTGAGAGTGCTTGATATTATTCTACGGCAGAAAGCAGCTAAGATGGGATGCCTTCTAGTACGACAGTCATTCTTCCATGATGACTCCAGGAACTTTGTTGAGATTGGAGGAGGTGTGACTGGTGTCCGGGGTTTTCATTCCAGTTTCCGTCCAACTCAGGGGGGCATGTCCTTGAATATGGATGTATCTACAACAATGATTGTGACGCCTGGACCTGTTATTGATTTTCTGTTAACCAACCAGGGTGTACGAGAACCCCGCTACATTGACTGGGTAAAGGCCAAAAGAATGCTGAAAAATATGAGGATTACAACGAGACACCGTAACATGGAGTGCAAAATCACTGGGTTGAGTGAGAAGACCTGCAATAATCAGTTGTTTAATCTGAAGCTAAGGAGTGGTGATGGCACGAGTGAGGGAGAAACTGTGGAGATTACGGTGTATGAATATTTCGTCAAACACTGTGGCATAGAGCTCACCTATTCTCAACACATGCCATGCCTTGATGTTGGCAAGCCAAAGAGGCCCACTTATGTTCCAGTTGAGCTATGTTCACTTGTTTCACTACAAAGGTATACAAAAGCATTATCTTCCCAGCAAAGAGCCTCCTTAGTGGAAAAATCAAGGCAGAAGCCTCTGGAGAGAATAAAGAGTGTGACTGATGCTTTAAGAAATTACCGCTATGATGAGGATCCTGTGCTTGCTGAATGTGGTATATCTATAGAGAAGCAACTGATTCAGATGGATGGCCGTATTCTTGAGACCCCAAAGTTAAAGGTAGGTGGCAGTGAGGACATGATGCCCTTTAAGGGACGATGGAACTTCAAAGGAAAGTCATTTTTGGATCCTACTAGCATTAACTCTTGGCTAGCTGTCAACTTCTCCGGACGCTGTGACCTTAGTCAAATCTCTCGGGAGCTTATCAATGTTGGGAAGAAGACTGGAATTCAAATTCAGCGGCCCAAAACTCTTATTGAGGAAGATCCGCGAGCTGAAAGACTAGGCCCTGTTGCTAGAGTAGACAAGATGTTTGAACAGATTCAGGCTAAGCTCCAAGAACCACCTGAGTTCATTCTGTGTGTCATACCCAAGAAAGGTTCTGATATCTATGGGCCTTGGAAGAAAAAAACTCTGGTTGACTTTGGGATTCCAACACAATGTATTTCCCCACCCAAGCTTAGCGATCCTTACCTGACTAATGTCCTAATGAAAATCAATACCAAGCTTGGAGGAATAAATTCTCTCTTGGCACTGGAGTACTCCTCACGTGTTCCTCTTATAAATGATACTCCTACAATGATTTTGGGGATGGATGTGTCTCATGGTTCTCCTGGCCGTTCAGATATTCCTTCAGTTGCTGCGGTTGTTGGCTCTCATAGTTGGCCTCTTATATCAAGGTACAGAGCAGCTGTTAGAACACAATCTCCTAAGGTGGAGATGATTGATGGTCTATACAAGCCACTTCCAAATGGAAATGATGATGGTATTATCAGGGAACTGCTTCTGGACTTCTATAACACAAGCAAAGGACGCAAACCAACTCAGATCATTGTTTTCAGGGATGGCGTCAGTGAATCACAGTTTAATCAAGTGTTGAACATTGAGCTGGATCAAATTGTAAAGGCTTACCAGCACCTTGGGGAGGAAAATGTTCCAAAGTTCACTCTCATTATAGCTCAAAAGAATCATCACACAAAGCTATTCCAAGCTAATAGCCCTGACAATGTTCCACCAGGGACAGTTGTTGACACAAAAATCGTGCACCCAAGGATCTATGATTTCTACATGTGTGCTCAGGCTGGCATGATTGGAACTTCGAGGCCAGCTCACTATCATGTCTTGCTTGATGAGATTGGCTTTTCCCCTGATGACTTGCAGAACCTGATTCACTCCCTTTCTTATGTGTACCAAAGGAGCTGTACTGCAATATCCATTGTGGCACCCATATGCTATGCGCACCTTGCTGCAGCACAAGTGGGGCAGTTTATGAAGTTTGAAGATCTCTCGGAAACTTCATCGGCTAAAGGTAGTGTCACAATATCAGAGAGCATCCCAGTTCCCGAGCTCCCTAGGCTTCATAGAAAGGTCCAGGGCTCCATGTTCTTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAR Jas NINJA_B

Protein Analysis

890

Amino Acids

99.59

Weight (kDa)

9.2

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 41 - 195 5.9e-31 N-terminal domain of argonaute
ArgoL1 PF08699 207 - 255 9.5e-18 Argonaute linker 1 domain
PAZ PF02170 261 - 391 4.9e-29 PAZ domain
ArgoL2 PF16488 401 - 446 5.3e-12 Argonaute linker 2 domain
ArgoMid PF16487 454 - 512 7.7e-06 Mid domain of argonaute
Piwi PF02171 544 - 851 1.6e-104 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1841
Acc16I TGCGCA 1 cut(s) 2517
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 1 cut(s) 2500
AccI GTMKAC 3 cut(s) 543, 1136, 1581
AccII CGCG 2 cut(s) 352, 1549
AccIII TCCGGA 1 cut(s) 1445
AciI CCGC 8 cut(s) 17, 121, 350, 820, 1240, 1519, 1547, 1922
AclWI GGATC 8 cut(s) 1247, 1260, 1400, 1413, 1538, 1739, 2178, 2339
AcoI YGGCCR 2 cut(s) 1309, 1894
AcsI RAATTY 3 cut(s) 1506, 1512, 1798
AcuI CTGAAG 4 cut(s) 267, 558, 968, 1896
AcvI CACGTG 2 cut(s) 101, 1829
AcyI GRCGYC 2 cut(s) 767, 2127
AfaI GTAC 8 cut(s) 33, 191, 621, 810, 1820, 1961, 2468, 2482
AfiI CCNNNNNNNGG 7 cut(s) 120, 230, 719, 889, 1370, 1655, 2203
AflIII ACRYGT 2 cut(s) 1828, 2346
AjnI CCWGG 6 cut(s) 647, 769, 801, 1891, 2293, 2649
AloI GAACNNNNNNTCC 2 cut(s) 1969, 2001
Alw21I GWGCWC 5 cut(s) 140, 1038, 2325, 2356, 2628
Alw26I GTCTC 5 cut(s) 214, 419, 871, 1316, 1884
Alw44I GTGCAC 1 cut(s) 2321
AlwI GGATC 8 cut(s) 1247, 1260, 1400, 1413, 1538, 1739, 2178, 2339
AlwNI CAGNNNCTG 3 cut(s) 176, 1919, 2443
Ama87I CYCGRG 2 cut(s) 1473, 2621
Aor13HI TCCGGA 1 cut(s) 1445
ApaLI GTGCAC 1 cut(s) 2321
ApeKI GCWGC 6 cut(s) 491, 596, 1919, 1967, 2525, 2528
ApoI RAATTY 3 cut(s) 1506, 1512, 1798
ArsI GACNNNNNNTTYG 2 cut(s) 1574, 1606
Asp700I GAANNNNTTC 5 cut(s) 467, 1599, 1798, 2061, 2211
AspA2I CCTAGG 1 cut(s) 2630
AspLEI GCGC 2 cut(s) 126, 2518
AspS9I GGNCC 7 cut(s) 341, 773, 1086, 1521, 1565, 1673, 2647
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 3 cut(s) 434, 974, 1030
AsuNHI GCTAGC 1 cut(s) 1429
AvaI CYCGRG 2 cut(s) 1473, 2621
AvaII GGWCC 3 cut(s) 341, 773, 2647
AvrII CCTAGG 1 cut(s) 2630
AxyI CCTNAGG 1 cut(s) 1989
BaeGI GKGCMC 1 cut(s) 2325
BamHI GGATCC 2 cut(s) 1252, 1405
BanI GGYRCC 1 cut(s) 2500
BanII GRGCYC 3 cut(s) 1038, 2628, 2657
BauI CACGAG 1 cut(s) 970
BbrPI CACGTG 2 cut(s) 101, 1829
BbsI GAAGAC 2 cut(s) 926, 1505
Bbv12I GWGCWC 5 cut(s) 140, 1038, 2325, 2356, 2628
BbvI GCAGC 6 cut(s) 478, 608, 1906, 1979, 2512, 2540
BceAI ACGGC 3 cut(s) 602, 1296, 1881
BciT130I CCWGG 6 cut(s) 649, 771, 803, 1893, 2295, 2651
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 5 cut(s) 214, 419, 871, 1316, 1884
BfaI CTAG 6 cut(s) 617, 1413, 1430, 1562, 1575, 2631
BfmI CTRYAG 2 cut(s) 1281, 2526
BfuAI ACCTGC 1 cut(s) 932
BglI GCCNNNNNGGC 1 cut(s) 1084
BglII AGATCT 1 cut(s) 2560
BisI GCNGC 8 cut(s) 122, 492, 597, 1520, 1920, 1968, 2526, 2529
BlnI CCTAGG 1 cut(s) 2630
BlpI GCTNAGC 2 cut(s) 1608, 1739
BlsI GCNGC 8 cut(s) 123, 493, 598, 1521, 1921, 1969, 2527, 2530
BmcAI AGTACT 2 cut(s) 191, 1820
Bme1390I CCNGG 7 cut(s) 649, 689, 771, 803, 1893, 2295, 2651
Bme18I GGWCC 3 cut(s) 341, 773, 2647
BmeT110I CYCGRG 2 cut(s) 1473, 2621
BmgT120I GGNCC 7 cut(s) 341, 773, 1086, 1521, 1565, 1673, 2647
BmiI GGNNCC 6 cut(s) 69, 112, 1254, 1407, 2502, 2656
BmrFI CCNGG 7 cut(s) 649, 689, 771, 803, 1893, 2295, 2651
BmrI ACTGGG 3 cut(s) 841, 915, 2609
BmsI GCATC 6 cut(s) 163, 547, 599, 1213, 1350, 2619
BmtI GCTAGC 1 cut(s) 1433
BmuI ACTGGG 3 cut(s) 841, 915, 2609
BoxI GACNNNNGTC 3 cut(s) 683, 1461, 1760
BpiI GAAGAC 2 cut(s) 926, 1505
BplI GAGNNNNNCTC 4 cut(s) 1808, 1840, 2371, 2403
BpmI CTGGAG 3 cut(s) 631, 1220, 1835
Bpu10I CCTNAGC 3 cut(s) 75, 953, 2355
Bpu1102I GCTNAGC 2 cut(s) 1608, 1739
BpuEI CTTGAG 3 cut(s) 383, 491, 1340
BpuMI CCSGG 1 cut(s) 689
BsaAI YACGTR 2 cut(s) 101, 1829
BsaBI GATNNNNATC 1 cut(s) 1251
BsaHI GRCGYC 2 cut(s) 767, 2127
BsaI GGTCTC 2 cut(s) 214, 1316
BsaJI CCNNGG 8 cut(s) 688, 802, 1676, 2197, 2294, 2327, 2630, 2650
BsaWI WCCGGW 1 cut(s) 1445
BsaXI ACNNNNNCTCC 8 cut(s) 525, 555, 660, 690, 972, 1002, 1969, 1999
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 230, 719, 889, 1370, 1655, 2203
Bse1I ACTGG 9 cut(s) 187, 685, 702, 836, 910, 1100, 1507, 1818, 2615
Bse21I CCTNAGG 1 cut(s) 1989
Bse8I GATNNNNATC 1 cut(s) 1251
BseAI TCCGGA 1 cut(s) 1445
BseBI CCWGG 6 cut(s) 649, 771, 803, 1893, 2295, 2651
BseDI CCNNGG 8 cut(s) 688, 802, 1676, 2197, 2294, 2327, 2630, 2650
BseGI GGATG 9 cut(s) 444, 562, 614, 748, 1312, 1875, 1879, 2128, 2610
BseJI GATNNNNATC 1 cut(s) 1251
BseLI CCNNNNNNNGG 7 cut(s) 120, 230, 719, 889, 1370, 1655, 2203
BseMII CTCAG 5 cut(s) 566, 731, 1617, 2117, 2369
BseNI ACTGG 9 cut(s) 187, 685, 702, 836, 910, 1100, 1507, 1818, 2615
BseRI GAGGAG 4 cut(s) 60, 398, 684, 1813
BseSI GKGCMC 1 cut(s) 2325
BseXI GCAGC 6 cut(s) 478, 608, 1906, 1979, 2512, 2540
BseYI CCCAGC 1 cut(s) 1155
Bsh1236I CGCG 2 cut(s) 352, 1549
Bsh1285I CGRYCG 1 cut(s) 461
BshNI GGYRCC 1 cut(s) 2500
BsiEI CGRYCG 1 cut(s) 461
BsiHKAI GWGCWC 5 cut(s) 140, 1038, 2325, 2356, 2628
BsiHKCI CYCGRG 2 cut(s) 1473, 2621
BsiSI CCGG 2 cut(s) 688, 1446
BslFI GGGAC 3 cut(s) 252, 1386, 2311
BslI CCNNNNNNNGG 7 cut(s) 120, 230, 719, 889, 1370, 1655, 2203
BsmAI GTCTC 5 cut(s) 214, 419, 871, 1316, 1884
BsmFI GGGAC 3 cut(s) 252, 1386, 2311
BsmI GAATGC 1 cut(s) 855
Bso31I GGTCTC 2 cut(s) 214, 1316
BsoBI CYCGRG 2 cut(s) 1473, 2621
Bsp1286I GDGCHC 6 cut(s) 140, 1038, 2325, 2356, 2628, 2657
Bsp13I TCCGGA 1 cut(s) 1445
Bsp143I GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
Bsp1720I GCTNAGC 2 cut(s) 1608, 1739
BspACI CCGC 8 cut(s) 17, 121, 350, 820, 1240, 1519, 1547, 1922
BspCNI CTCAG 5 cut(s) 565, 730, 1618, 2116, 2368
BspEI TCCGGA 1 cut(s) 1445
BspFNI CGCG 2 cut(s) 352, 1549
BspLI GGNNCC 6 cut(s) 69, 112, 1254, 1407, 2502, 2656
BspMAI CTGCAG 1 cut(s) 2530
BspMI ACCTGC 1 cut(s) 932
BspOI GCTAGC 1 cut(s) 1433
BspPI GGATC 8 cut(s) 1247, 1260, 1400, 1413, 1538, 1739, 2178, 2339
BspT107I GGYRCC 1 cut(s) 2500
BspTNI GGTCTC 2 cut(s) 214, 1316
BsrI ACTGG 9 cut(s) 187, 685, 702, 836, 910, 1100, 1507, 1818, 2615
BssECI CCNNGG 8 cut(s) 688, 802, 1676, 2197, 2294, 2327, 2630, 2650
BssMI GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
BssNAI GTATAC 1 cut(s) 1137
BssNI GRCGYC 2 cut(s) 767, 2127
BssSI CACGAG 1 cut(s) 970
BssT1I CCWWGG 4 cut(s) 1676, 2197, 2327, 2630
Bst1107I GTATAC 1 cut(s) 1137
Bst2BI CACGAG 1 cut(s) 970
Bst2UI CCWGG 6 cut(s) 649, 771, 803, 1893, 2295, 2651
BstACI GRCGYC 2 cut(s) 767, 2127
BstBAI YACGTR 2 cut(s) 101, 1829
BstC8I GCNNGC 6 cut(s) 1076, 1263, 1431, 1551, 2362, 2385
BstENI CCTNNNNNAGG 2 cut(s) 228, 2201
BstF5I GGATG 9 cut(s) 444, 562, 614, 748, 1312, 1875, 1879, 2128, 2610
BstFNI CGCG 2 cut(s) 352, 1549
BstHHI GCGC 2 cut(s) 126, 2518
BstKTI GATC 8 cut(s) 1255, 1408, 1546, 1747, 2109, 2173, 2334, 2563
BstMAI GTCTC 5 cut(s) 214, 419, 871, 1316, 1884
BstMBI GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
BstMCI CGRYCG 1 cut(s) 461
BstMWI GCNNNNNNNGC 4 cut(s) 1084, 2091, 2522, 2632
BstNI CCWGG 6 cut(s) 649, 771, 803, 1893, 2295, 2651
BstNSI RCATGY 3 cut(s) 730, 1057, 2350
BstPAI GACNNNNGTC 3 cut(s) 683, 1461, 1760
BstSCI CCNGG 7 cut(s) 647, 687, 769, 801, 1891, 2293, 2649
BstSFI CTRYAG 2 cut(s) 1281, 2526
BstSLI GKGCMC 1 cut(s) 2325
BstUI CGCG 2 cut(s) 352, 1549
BstV1I GCAGC 6 cut(s) 478, 608, 1906, 1979, 2512, 2540
BstV2I GAAGAC 2 cut(s) 926, 1505
BstX2I RGATCY 5 cut(s) 1252, 1405, 1543, 2331, 2560
BstXI CCANNNNNNTGG 1 cut(s) 2198
BstYI RGATCY 5 cut(s) 1252, 1405, 1543, 2331, 2560
BstZ17I GTATAC 1 cut(s) 1137
Bsu36I CCTNAGG 1 cut(s) 1989
BtsCI GGATG 9 cut(s) 444, 562, 614, 748, 1312, 1875, 1879, 2128, 2610
BtsI GCAGTG 1 cut(s) 1354
BtsIMutI CAGTG 6 cut(s) 445, 903, 1021, 1354, 1811, 2137
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 6 cut(s) 1076, 1263, 1431, 1551, 2362, 2385
CaiI CAGNNNCTG 3 cut(s) 176, 1919, 2443
CfoI GCGC 2 cut(s) 126, 2518
Cfr13I GGNCC 7 cut(s) 341, 773, 1086, 1521, 1565, 1673, 2647
CseI GACGC 4 cut(s) 775, 1458, 2100, 2116
Csp6I GTAC 8 cut(s) 32, 190, 620, 809, 1819, 1960, 2467, 2481
CspCI CAANNNNNGTGG 2 cut(s) 1006, 1041
CviQI GTAC 8 cut(s) 32, 190, 620, 809, 1819, 1960, 2467, 2481
DpnI GATC 8 cut(s) 1254, 1407, 1545, 1746, 2108, 2172, 2333, 2562
DpnII GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
EaeI YGGCCR 2 cut(s) 1309, 1894
EciI GGCGGA 1 cut(s) 6
Ecl136II GAGCTC 2 cut(s) 1036, 2626
Eco130I CCWWGG 4 cut(s) 1676, 2197, 2327, 2630
Eco24I GRGCYC 3 cut(s) 1038, 2628, 2657
Eco31I GGTCTC 2 cut(s) 214, 1316
Eco32I GATATC 1 cut(s) 1666
Eco47I GGWCC 3 cut(s) 341, 773, 2647
Eco53kI GAGCTC 2 cut(s) 1036, 2626
Eco57I CTGAAG 4 cut(s) 267, 558, 968, 1896
Eco72I CACGTG 2 cut(s) 101, 1829
Eco81I CCTNAGG 1 cut(s) 1989
Eco88I CYCGRG 2 cut(s) 1473, 2621
EcoICRI GAGCTC 2 cut(s) 1036, 2626
EcoNI CCTNNNNNAGG 2 cut(s) 228, 2201
EcoO109I RGGNCCY 1 cut(s) 1565
EcoRI GAATTC 1 cut(s) 1506
EcoRII CCWGG 6 cut(s) 647, 769, 801, 1891, 2293, 2649
EcoRV GATATC 1 cut(s) 1666
EcoT14I CCWWGG 4 cut(s) 1676, 2197, 2327, 2630
EcoT22I ATGCAT 1 cut(s) 562
EcoT38I GRGCYC 3 cut(s) 1038, 2628, 2657
ErhI CCWWGG 4 cut(s) 1676, 2197, 2327, 2630
FaqI GGGAC 3 cut(s) 252, 1386, 2311
FauI CCCGC 2 cut(s) 357, 827
FauNDI CATATG 2 cut(s) 310, 2507
FblI GTMKAC 3 cut(s) 543, 1136, 1581
Fnu4HI GCNGC 8 cut(s) 122, 492, 597, 1520, 1920, 1968, 2526, 2529
FokI GGATG 9 cut(s) 451, 569, 621, 755, 1319, 1882, 1886, 2135, 2597
FriOI GRGCYC 3 cut(s) 1038, 2628, 2657
Fsp4HI GCNGC 8 cut(s) 122, 492, 597, 1520, 1920, 1968, 2526, 2529
FspAI RTGCGCAY 1 cut(s) 2517
FspBI CTAG 6 cut(s) 617, 1413, 1430, 1562, 1575, 2631
FspI TGCGCA 1 cut(s) 2517
GlaI GCGC 2 cut(s) 125, 2517
GluI GCNGC 8 cut(s) 122, 492, 597, 1520, 1920, 1968, 2526, 2529
GsaI CCCAGC 1 cut(s) 1159
GsuI CTGGAG 3 cut(s) 631, 1220, 1835
HapII CCGG 2 cut(s) 688, 1446
HgaI GACGC 4 cut(s) 775, 1458, 2100, 2116
HhaI GCGC 2 cut(s) 126, 2518
Hin1I GRCGYC 2 cut(s) 767, 2127
Hin6I GCGC 2 cut(s) 124, 2516
HinP1I GCGC 2 cut(s) 124, 2516
HincII GTYRAC 4 cut(s) 795, 1438, 1699, 2308
HindII GTYRAC 4 cut(s) 795, 1438, 1699, 2308
HindIII AAGCTT 3 cut(s) 324, 1736, 1784
HinfI GANTC 9 cut(s) 166, 386, 644, 1297, 1600, 1708, 2135, 2245, 2446
HpaI GTTAAC 1 cut(s) 795
HpaII CCGG 2 cut(s) 688, 1446
HphI GGTGA 3 cut(s) 434, 974, 1030
Hpy188I TCNGA 9 cut(s) 201, 286, 948, 1302, 1663, 1903, 2106, 2569, 2605
Hpy188III TCNNGA 8 cut(s) 170, 554, 1199, 1319, 1446, 1475, 2066, 2621
HpyAV CCTTC 4 cut(s) 224, 536, 623, 1920
HpyCH4IV ACGT 2 cut(s) 100, 1828
HpyCH4V TGCA 8 cut(s) 308, 560, 897, 927, 2323, 2437, 2486, 2528
HpyF10VI GCNNNNNNNGC 4 cut(s) 1084, 2091, 2522, 2632
HpySE526I ACGT 2 cut(s) 100, 1828
Hsp92I GRCGYC 2 cut(s) 767, 2127
HspAI GCGC 2 cut(s) 124, 2516
Kpn2I TCCGGA 1 cut(s) 1445
KspAI GTTAAC 1 cut(s) 795
Kzo9I GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
LmnI GCTCC 7 cut(s) 73, 533, 1477, 1617, 2475, 2631, 2660
Lsp1109I GCAGC 6 cut(s) 478, 608, 1906, 1979, 2512, 2540
LweI GCATC 6 cut(s) 163, 547, 599, 1213, 1350, 2619
MaeI CTAG 6 cut(s) 617, 1413, 1430, 1562, 1575, 2631
MaeII ACGT 2 cut(s) 100, 1828
MaeIII GTNAC 6 cut(s) 676, 763, 884, 1216, 1454, 2593
MalI GATC 8 cut(s) 1254, 1407, 1545, 1746, 2108, 2172, 2333, 2562
MboI GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
MfeI CAATTG 2 cut(s) 131, 513
MflI RGATCY 5 cut(s) 1252, 1405, 1543, 2331, 2560
MhlI GDGCHC 6 cut(s) 140, 1038, 2325, 2356, 2628, 2657
MluCI AATT 8 cut(s) 131, 408, 513, 1234, 1506, 1512, 1798, 2175
MlyI GAGTC 2 cut(s) 395, 638
MmeI TCCRAC 2 cut(s) 737, 1736
Mph1103I ATGCAT 1 cut(s) 562
MroI TCCGGA 1 cut(s) 1445
MroXI GAANNNNTTC 5 cut(s) 467, 1599, 1798, 2061, 2211
MseI TTAA 7 cut(s) 794, 942, 1229, 1334, 1368, 1419, 2145
MslI CAYNNNNRTG 2 cut(s) 2394, 2506
MspA1I CMGCKG 2 cut(s) 1519, 1970
MspI CCGG 2 cut(s) 688, 1446
MspR9I CCNGG 7 cut(s) 649, 689, 771, 803, 1893, 2295, 2651
MunI CAATTG 2 cut(s) 131, 513
Mva1269I GAATGC 1 cut(s) 855
MvaI CCWGG 6 cut(s) 649, 771, 803, 1893, 2295, 2651
MvnI CGCG 2 cut(s) 352, 1549
MwoI GCNNNNNNNGC 4 cut(s) 1084, 2091, 2522, 2632
NciI CCSGG 1 cut(s) 689
NdeI CATATG 2 cut(s) 310, 2507
NdeII GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
NheI GCTAGC 1 cut(s) 1429
NlaIV GGNNCC 6 cut(s) 69, 112, 1254, 1407, 2502, 2656
NmuCI GTSAC 5 cut(s) 676, 763, 1216, 1454, 2593
NsbI TGCGCA 1 cut(s) 2517
NsiI ATGCAT 1 cut(s) 562
NspI RCATGY 3 cut(s) 730, 1057, 2350
PciI ACATGT 1 cut(s) 2346
PctI GAATGC 1 cut(s) 855
PdmI GAANNNNTTC 5 cut(s) 467, 1599, 1798, 2061, 2211
PfeI GAWTC 7 cut(s) 166, 1297, 1600, 1708, 2135, 2245, 2446
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 8 cut(s) 123, 493, 598, 1521, 1921, 1969, 2527, 2530
PleI GAGTC 2 cut(s) 394, 638
PmaCI CACGTG 2 cut(s) 101, 1829
PmlI CACGTG 2 cut(s) 101, 1829
PpsI GAGTC 2 cut(s) 394, 638
Ppu21I YACGTR 2 cut(s) 101, 1829
PscI ACATGT 1 cut(s) 2346
PshAI GACNNNNGTC 3 cut(s) 683, 1461, 1760
PsiI TTATAA 1 cut(s) 1841
Psp124BI GAGCTC 2 cut(s) 1038, 2628
Psp6I CCWGG 6 cut(s) 647, 769, 801, 1891, 2293, 2649
PspCI CACGTG 2 cut(s) 101, 1829
PspFI CCCAGC 1 cut(s) 1155
PspGI CCWGG 6 cut(s) 647, 769, 801, 1891, 2293, 2649
PspN4I GGNNCC 6 cut(s) 69, 112, 1254, 1407, 2502, 2656
PspPI GGNCC 7 cut(s) 341, 773, 1086, 1521, 1565, 1673, 2647
PstI CTGCAG 1 cut(s) 2530
PstNI CAGNNNCTG 3 cut(s) 176, 1919, 2443
PsuI RGATCY 5 cut(s) 1252, 1405, 1543, 2331, 2560
PvuII CAGCTG 1 cut(s) 1970
RsaI GTAC 8 cut(s) 33, 191, 621, 810, 1820, 1961, 2468, 2482
RsaNI GTAC 8 cut(s) 32, 190, 620, 809, 1819, 1960, 2467, 2481
RseI CAYNNNNRTG 2 cut(s) 2394, 2506
SacI GAGCTC 2 cut(s) 1038, 2628
SaqAI TTAA 7 cut(s) 794, 942, 1229, 1334, 1368, 1419, 2145
SatI GCNGC 8 cut(s) 122, 492, 597, 1520, 1920, 1968, 2526, 2529
Sau3AI GATC 8 cut(s) 1252, 1405, 1543, 1744, 2106, 2170, 2331, 2560
Sau96I GGNCC 7 cut(s) 341, 773, 1086, 1521, 1565, 1673, 2647
ScaI AGTACT 2 cut(s) 191, 1820
SchI GAGTC 2 cut(s) 395, 638
ScrFI CCNGG 7 cut(s) 649, 689, 771, 803, 1893, 2295, 2651
SduI GDGCHC 6 cut(s) 140, 1038, 2325, 2356, 2628, 2657
SfaNI GCATC 6 cut(s) 163, 547, 599, 1213, 1350, 2619
SfcI CTRYAG 2 cut(s) 1281, 2526
SinI GGWCC 3 cut(s) 341, 773, 2647
SmiMI CAYNNNNRTG 2 cut(s) 2394, 2506
SmlI CTYRAG 3 cut(s) 362, 506, 1319
SmoI CTYRAG 3 cut(s) 362, 506, 1319
Sse9I AATT 8 cut(s) 131, 408, 513, 1234, 1506, 1512, 1798, 2175
SsiI CCGC 8 cut(s) 17, 121, 350, 820, 1240, 1519, 1547, 1922
SspI AATATT 1 cut(s) 1010
SspMI CTAG 6 cut(s) 617, 1413, 1430, 1562, 1575, 2631
SstI GAGCTC 2 cut(s) 1038, 2628
StyD4I CCNGG 7 cut(s) 647, 687, 769, 801, 1891, 2293, 2649
StyI CCWWGG 4 cut(s) 1676, 2197, 2327, 2630
TaiI ACGT 2 cut(s) 103, 1831
TaqI TCGA 2 cut(s) 461, 2378
TaqII GACCGA 2 cut(s) 329, 447
TasI AATT 8 cut(s) 131, 408, 513, 1234, 1506, 1512, 1798, 2175
TatI WGTACW 3 cut(s) 189, 1818, 2480
TauI GCSGC 2 cut(s) 124, 1522
TfiI GAWTC 7 cut(s) 166, 1297, 1600, 1708, 2135, 2245, 2446
Tru1I TTAA 7 cut(s) 794, 942, 1229, 1334, 1368, 1419, 2145
Tru9I TTAA 7 cut(s) 794, 942, 1229, 1334, 1368, 1419, 2145
TscAI CASTG 6 cut(s) 452, 910, 1028, 1354, 1818, 2137
TseFI GTSAC 5 cut(s) 676, 763, 1216, 1454, 2593
TseI GCWGC 6 cut(s) 491, 596, 1919, 1967, 2525, 2528
Tsp45I GTSAC 5 cut(s) 676, 763, 1216, 1454, 2593
TspDTI ATGAA 9 cut(s) 225, 455, 686, 1020, 1621, 1784, 2564, 2565, 2627
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 6 cut(s) 452, 910, 1028, 1354, 1818, 2137
VneI GTGCAC 1 cut(s) 2321
VpaK11BI GGWCC 3 cut(s) 341, 773, 2647
XagI CCTNNNNNAGG 2 cut(s) 228, 2201
XapI RAATTY 3 cut(s) 1506, 1512, 1798
XceI RCATGY 3 cut(s) 730, 1057, 2350
XmaJI CCTAGG 1 cut(s) 2630
XmiI GTMKAC 3 cut(s) 543, 1136, 1581
XmnI GAANNNNTTC 5 cut(s) 467, 1599, 1798, 2061, 2211
XspI CTAG 6 cut(s) 617, 1413, 1430, 1562, 1575, 2631
ZrmI AGTACT 2 cut(s) 191, 1820
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.