pycom06g18020

Ninja-family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002373
Physical Location & Seq
Reverse (-)
21907723 .. 21910668
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18020.2

Sequence Viewer

Length: 870 bp
ATGGCCGCCGCAAACAAAGACAACCTTTCTCAACCCAACAACGCCAACATTCAAAACAACACCGTCGATTCCCAAATCCCGGACCTCAACCTGGAAATCTCTCTCAGCGGGATTTACACCGATAATTCCAACCAAAACCCTCTCGACCGGTCCTCCACGGAGGCCAGGGTCTTGAGGCTTCAAGAAACCCAAAAGGCGCCGCAGATGGCTGCTGCTCCGATTCGTGCTATACGGCTTCCAAGGGCAAGCTCTCTTCCATCAGAGTCCAGGTTGGCGATCAGGCACAAGGACGTTCTAGCCTTGAGAAGGCCGGAGACCAAACAGAAGATAGCTCAGAGGCAGATGAGCCAGCAACCAGCTGCTTTGCCGGAGGTGGCTAGGGCTTTAGCAGCTCCGGCGGCTCCAGCAGATCTGGCGGTCTGTAGTGCGCCTTGTGCTTCTAACGGTGCCGGGGCTTTGGATACAGTCTATCATTACAAAGAGGAGGGATATTTCACTGTGTCTCGTGCTGCAGAGGGTGCGGTAAGTGTTCAGACGTTGAATTCCCAAAGGAATGTTACACCAGTAGCCACGTCTGTAACAGCGGCCACTACAGAAGAGGTTGGCCCTTCACAAAGTACACCAAAGAGGGTCAAACATTCCACTGGAAGTGTTCCCCAGAATGATGGGGACGATATGGTTTCAGTGAGAACCACTGGAGAAGGGGGAAGACAGATAGAAGGAATATTATATGCCCGTGATGGCGAGGTCCACATTTTGTGTGTCTGTCATGGCCTCTTCCACTCCCCTGCCGAATTCATTAAGCATGGCGGTGGAAAGGAAGTCGCCAATCCTTTGCGGCATATATTTGTCCGCCCTTCTTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
EAR Jas NINJA_B

Protein Analysis

290

Amino Acids

30.88

Weight (kDa)

7.75

Isoelectric Point (pI)

61.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 196, 446
AcoI YGGCCR 2 cut(s) 3, 585
AcsI RAATTY 2 cut(s) 541, 794
AcyI GRCGYC 1 cut(s) 197
AfaI GTAC 1 cut(s) 619
AfiI CCNNNNNNNGG 3 cut(s) 79, 91, 306
AgeI ACCGGT 1 cut(s) 147
AgsI TTSAA 3 cut(s) 53, 182, 541
AjiI CACGTC 1 cut(s) 573
AjnI CCWGG 3 cut(s) 90, 164, 266
AluBI AGCT 4 cut(s) 249, 332, 359, 392
AluI AGCT 4 cut(s) 249, 332, 359, 392
Alw26I GTCTC 2 cut(s) 308, 507
AoxI GGCC 6 cut(s) 3, 162, 308, 585, 604, 772
ApeKI GCWGC 5 cut(s) 209, 212, 359, 389, 509
ApoI RAATTY 2 cut(s) 541, 794
AsiGI ACCGGT 1 cut(s) 147
AspLEI GCGC 2 cut(s) 199, 430
AspS9I GGNCC 4 cut(s) 82, 150, 605, 748
AsuC2I CCSGG 2 cut(s) 80, 451
AvaII GGWCC 3 cut(s) 82, 150, 748
BanI GGYRCC 2 cut(s) 196, 446
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 715
BbvI GCAGC 5 cut(s) 196, 199, 346, 401, 496
BccI CCATC 4 cut(s) 199, 265, 659, 734
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 3 cut(s) 92, 166, 268
BciVI GTATCC 1 cut(s) 454
BcnI CCSGG 2 cut(s) 80, 451
BcoDI GTCTC 2 cut(s) 308, 507
BfaI CTAG 2 cut(s) 296, 378
BfmI CTRYAG 3 cut(s) 421, 510, 591
BfoI RGCGCY 1 cut(s) 200
BfuI GTATCC 1 cut(s) 454
BglII AGATCT 1 cut(s) 409
Bme1390I CCNGG 5 cut(s) 80, 92, 166, 268, 451
Bme18I GGWCC 3 cut(s) 82, 150, 748
BmgBI CACGTC 1 cut(s) 573
BmgT120I GGNCC 4 cut(s) 82, 150, 605, 748
BmiI GGNNCC 3 cut(s) 198, 402, 448
BmrFI CCNGG 5 cut(s) 80, 92, 166, 268, 451
BpiI GAAGAC 1 cut(s) 715
BpmI CTGGAG 2 cut(s) 387, 717
BpuEI CTTGAG 2 cut(s) 193, 322
BpuMI CCSGG 2 cut(s) 80, 451
BsaHI GRCGYC 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 4 cut(s) 156, 165, 239, 450
BsaWI WCCGGW 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 91, 306
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 3 cut(s) 563, 649, 700
BseBI CCWGG 3 cut(s) 92, 166, 268
BseDI CCNNGG 4 cut(s) 156, 165, 239, 450
BseLI CCNNNNNNNGG 3 cut(s) 79, 91, 306
BseMII CTCAG 2 cut(s) 118, 347
BseNI ACTGG 3 cut(s) 563, 649, 700
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 5 cut(s) 196, 199, 346, 401, 496
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 6 cut(s) 5, 164, 310, 587, 606, 774
BshNI GGYRCC 2 cut(s) 196, 446
BshTI ACCGGT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 148
BsiSI CCGG 6 cut(s) 80, 148, 311, 368, 395, 450
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 3 cut(s) 79, 91, 306
BsmAI GTCTC 2 cut(s) 308, 507
BsmFI GGGAC 1 cut(s) 683
BsnI GGCC 6 cut(s) 5, 164, 310, 587, 606, 774
Bso31I GGTCTC 1 cut(s) 308
Bsp143I GATC 2 cut(s) 276, 409
BspANI GGCC 6 cut(s) 5, 164, 310, 587, 606, 774
BspCNI CTCAG 2 cut(s) 117, 346
BspLI GGNNCC 3 cut(s) 198, 402, 448
BspMAI CTGCAG 1 cut(s) 514
BspT107I GGYRCC 2 cut(s) 196, 446
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 3 cut(s) 563, 649, 700
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 4 cut(s) 156, 165, 239, 450
BssMI GATC 2 cut(s) 276, 409
BssNI GRCGYC 1 cut(s) 197
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 3 cut(s) 92, 166, 268
Bst4CI ACNGT 4 cut(s) 64, 446, 466, 499
Bst6I CTCTTC 3 cut(s) 258, 591, 782
BstACI GRCGYC 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 247, 350
BstDEI CTNAG 2 cut(s) 104, 333
BstDSI CCRYGG 1 cut(s) 156
BstENI CCTNNNNNAGG 1 cut(s) 304
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 2 cut(s) 199, 430
BstKTI GATC 2 cut(s) 279, 412
BstMAI GTCTC 2 cut(s) 308, 507
BstMBI GATC 2 cut(s) 276, 409
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 7 cut(s) 389, 395, 398, 404, 413, 434, 518
BstNI CCWGG 3 cut(s) 92, 166, 268
BstSCI CCNGG 5 cut(s) 78, 90, 164, 266, 449
BstSFI CTRYAG 3 cut(s) 421, 510, 591
BstV1I GCAGC 5 cut(s) 196, 199, 346, 401, 496
BstV2I GAAGAC 1 cut(s) 715
BstX2I RGATCY 1 cut(s) 409
BstXI CCANNNNNNTGG 1 cut(s) 665
BstYI RGATCY 1 cut(s) 409
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 6 cut(s) 5, 164, 310, 587, 606, 774
BtgI CCRYGG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 573
BtsIMutI CAGTG 4 cut(s) 495, 642, 690, 693
Cac8I GCNNGC 2 cut(s) 247, 350
CfoI GCGC 2 cut(s) 199, 430
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 4 cut(s) 82, 150, 605, 748
Csp6I GTAC 1 cut(s) 618
CspAI ACCGGT 1 cut(s) 147
CviAII CATG 2 cut(s) 770, 806
CviQI GTAC 1 cut(s) 618
DdeI CTNAG 2 cut(s) 104, 333
DinI GGCGCC 1 cut(s) 198
DpnI GATC 2 cut(s) 278, 411
DpnII GATC 2 cut(s) 276, 409
EaeI YGGCCR 2 cut(s) 3, 585
Eam1104I CTCTTC 3 cut(s) 258, 591, 782
EarI CTCTTC 3 cut(s) 258, 591, 782
EciI GGCGGA 1 cut(s) 842
Eco130I CCWWGG 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 3 cut(s) 82, 150, 748
EcoNI CCTNNNNNAGG 1 cut(s) 304
EcoRI GAATTC 2 cut(s) 541, 794
EcoRII CCWGG 3 cut(s) 90, 164, 266
EcoT14I CCWWGG 1 cut(s) 239
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 2 cut(s) 773, 809
FaiI YATR 8 cut(s) 230, 677, 730, 732, 771, 807, 843, 845
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FaqI GGGAC 1 cut(s) 683
FatI CATG 2 cut(s) 769, 805
FauI CCCGC 1 cut(s) 101
FspBI CTAG 2 cut(s) 296, 378
GlaI GCGC 2 cut(s) 198, 429
GsuI CTGGAG 2 cut(s) 387, 717
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 6 cut(s) 5, 164, 310, 587, 606, 774
HapII CCGG 6 cut(s) 80, 148, 311, 368, 395, 450
HhaI GCGC 2 cut(s) 199, 430
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 2 cut(s) 773, 809
Hin6I GCGC 2 cut(s) 197, 428
HinP1I GCGC 2 cut(s) 197, 428
HinfI GANTC 3 cut(s) 68, 220, 263
HpaII CCGG 6 cut(s) 80, 148, 311, 368, 395, 450
Hpy166II GTNNAC 2 cut(s) 620, 751
Hpy188I TCNGA 5 cut(s) 219, 262, 336, 534, 865
Hpy188III TCNNGA 3 cut(s) 143, 172, 182
Hpy8I GTNNAC 2 cut(s) 620, 751
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 5 cut(s) 300, 618, 695, 713, 867
HpyCH4III ACNGT 4 cut(s) 64, 446, 466, 499
HpyCH4IV ACGT 3 cut(s) 291, 536, 572
HpyCH4V TGCA 1 cut(s) 512
HpyF10VI GCNNNNNNNGC 7 cut(s) 389, 395, 398, 404, 413, 434, 518
HpyF3I CTNAG 2 cut(s) 104, 333
HpySE526I ACGT 3 cut(s) 291, 536, 572
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 2 cut(s) 773, 809
HspAI GCGC 2 cut(s) 197, 428
KasI GGCGCC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 276, 409
LmnI GCTCC 3 cut(s) 220, 397, 406
Lsp1109I GCAGC 5 cut(s) 196, 199, 346, 401, 496
MaeI CTAG 2 cut(s) 296, 378
MaeII ACGT 3 cut(s) 291, 536, 572
MaeIII GTNAC 2 cut(s) 556, 577
MalI GATC 2 cut(s) 278, 411
MboI GATC 2 cut(s) 276, 409
MboII GAAGA 6 cut(s) 245, 337, 608, 720, 769, 852
MflI RGATCY 1 cut(s) 409
MluCI AATT 3 cut(s) 124, 541, 794
Mly113I GGCGCC 1 cut(s) 197
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 153
MseI TTAA 1 cut(s) 801
MslI CAYNNNNRTG 1 cut(s) 810
MspA1I CMGCKG 3 cut(s) 108, 359, 584
MspI CCGG 6 cut(s) 80, 148, 311, 368, 395, 450
MspR9I CCNGG 5 cut(s) 80, 92, 166, 268, 451
MvaI CCWGG 3 cut(s) 92, 166, 268
MwoI GCNNNNNNNGC 7 cut(s) 389, 395, 398, 404, 413, 434, 518
NarI GGCGCC 1 cut(s) 197
NciI CCSGG 2 cut(s) 80, 451
NdeII GATC 2 cut(s) 276, 409
NlaIII CATG 2 cut(s) 773, 809
NlaIV GGNNCC 3 cut(s) 198, 402, 448
PfeI GAWTC 2 cut(s) 68, 220
PfoI TCCNGGA 1 cut(s) 78
PinAI ACCGGT 1 cut(s) 147
PleI GAGTC 1 cut(s) 271
PluTI GGCGCC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 271
Psp6I CCWGG 3 cut(s) 90, 164, 266
PspGI CCWGG 3 cut(s) 90, 164, 266
PspN4I GGNNCC 3 cut(s) 198, 402, 448
PspPI GGNCC 4 cut(s) 82, 150, 605, 748
PstI CTGCAG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 619
RsaNI GTAC 1 cut(s) 618
RseI CAYNNNNRTG 1 cut(s) 810
SaqAI TTAA 1 cut(s) 801
Sau3AI GATC 2 cut(s) 276, 409
Sau96I GGNCC 4 cut(s) 82, 150, 605, 748
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 5 cut(s) 80, 92, 166, 268, 451
SfcI CTRYAG 3 cut(s) 421, 510, 591
SfoI GGCGCC 1 cut(s) 198
SinI GGWCC 3 cut(s) 82, 150, 748
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 2 cut(s) 172, 301
SmoI CTYRAG 2 cut(s) 172, 301
Sse9I AATT 3 cut(s) 124, 541, 794
SspDI GGCGCC 1 cut(s) 196
SspI AATATT 1 cut(s) 726
SspMI CTAG 2 cut(s) 296, 378
StyD4I CCNGG 5 cut(s) 78, 90, 164, 266, 449
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 4 cut(s) 64, 446, 466, 499
TaiI ACGT 3 cut(s) 294, 539, 575
TaqI TCGA 2 cut(s) 66, 144
TasI AATT 3 cut(s) 124, 541, 794
TatI WGTACW 1 cut(s) 617
TauI GCSGC 6 cut(s) 8, 11, 202, 401, 587, 841
TfiI GAWTC 2 cut(s) 68, 220
Tru1I TTAA 1 cut(s) 801
Tru9I TTAA 1 cut(s) 801
TscAI CASTG 4 cut(s) 502, 649, 690, 700
TseI GCWGC 5 cut(s) 209, 212, 359, 389, 509
TspDTI ATGAA 1 cut(s) 787
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 4 cut(s) 502, 649, 690, 700
VpaK11BI GGWCC 3 cut(s) 82, 150, 748
XagI CCTNNNNNAGG 1 cut(s) 304
XapI RAATTY 2 cut(s) 541, 794
XspI CTAG 2 cut(s) 296, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.