FvH4_5g13760

Ninja-family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002373
Physical Location & Seq
Forward (+)
7777026 .. 7780137
3112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13760.t1

Sequence Viewer

Length: 705 bp
ATGGCGGGACAGCAGGAGGAGTACGATCTCGGGCTCTCACTTGGTGGACTGTACAAGCGAAACTCCGCTGGGGCCCACAGGGCACGCGCTGTGTCGGAGGAGAATGCGGTGGCGGTGGCGGCGGCGCCGAGGGCCGGCAGGGCGGCTTCTCTGCCGACGGAAAGAGAGATGGAGTTGATGAGGGAGGGGCTGACTGAAGTTCAAGCTCTTAGCAGGGTGATGGCTGCAAGCTTGAAGCTGACTATTCAGAAGGAGAGGACTAACGGAGCGCCGGCGGTGGCGGCCTGGCTGCCTGGACTGGTTTCTGCAGGAGGATCAGGACGCCTGGCTGCAACAAGCAGCACAGTCAGTGGTGAGCTTATGTCTACAGACGGGAGCTCCAACCGGCCTGCAGTGACCAGCTCAATGATAGCTAAAGAGAATGCTGATGACCCTCAACAAGATCCACCAAGGAGGGATAGACTCTATAATCGCAATGTTGCAAGGCCGATTAGGCGCTATAGGAGGAACACCATTATGCCTCATGTGAAAACCATTAGTGCTGATGGGAAGAGGGTCGAGGGCTATTTGTACAAGTATCTTACAGGAAAAGTGAGAATCGTATGTCTTTGCCATGGCCTTTTCCAGTCTCCAGCAGAGTTTGTCAACCACGCCGGAGGCAATGTGGCCGGAAACCCCTTGAAGCAAATCACTGTTTCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
EAR Jas NINJA_B

Protein Analysis

235

Amino Acids

25.09

Weight (kDa)

9.9

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 197 - 231 1e-06 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 365
AccII CGCG 1 cut(s) 87
AciI CCGC 9 cut(s) 5, 66, 107, 113, 119, 122, 143, 275, 281
AclWI GGATC 2 cut(s) 322, 437
AcoI YGGCCR 1 cut(s) 666
AcuI CTGAAG 1 cut(s) 216
AcyI GRCGYC 2 cut(s) 125, 322
AdeI CACNNNGTG 1 cut(s) 44
AfaI GTAC 3 cut(s) 23, 53, 572
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 3 cut(s) 203, 235, 682
AjnI CCWGG 3 cut(s) 284, 292, 324
AluBI AGCT 7 cut(s) 206, 231, 238, 358, 378, 402, 413
AluI AGCT 7 cut(s) 206, 231, 238, 358, 378, 402, 413
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 2 cut(s) 322, 437
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 7 cut(s) 72, 132, 282, 386, 485, 616, 666
ApaI GGGCCC 1 cut(s) 76
ApeKI GCWGC 4 cut(s) 224, 289, 329, 339
AspLEI GCGC 4 cut(s) 89, 127, 271, 498
AspS9I GGNCC 3 cut(s) 72, 73, 132
AsuHPI GGTGA 2 cut(s) 229, 365
AvaI CYCGRG 1 cut(s) 29
BaeGI GKGCMC 2 cut(s) 76, 85
BanI GGYRCC 1 cut(s) 124
BanII GRGCYC 3 cut(s) 36, 76, 380
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 4 cut(s) 211, 276, 316, 351
BccI CCATC 3 cut(s) 163, 214, 539
BciT130I CCWGG 3 cut(s) 286, 294, 326
BcoDI GTCTC 1 cut(s) 633
BfmI CTRYAG 4 cut(s) 306, 366, 390, 499
BfoI RGCGCY 3 cut(s) 128, 272, 499
BglI GCCNNNNNGGC 3 cut(s) 80, 140, 493
BisI GCNGC 8 cut(s) 120, 123, 144, 225, 282, 290, 330, 340
BlsI GCNGC 8 cut(s) 121, 124, 145, 226, 283, 291, 331, 341
Bme1390I CCNGG 3 cut(s) 286, 294, 326
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 3 cut(s) 72, 73, 132
BmiI GGNNCC 3 cut(s) 73, 74, 126
BmrFI CCNGG 3 cut(s) 286, 294, 326
BpmI CTGGAG 1 cut(s) 615
BsaHI GRCGYC 2 cut(s) 125, 322
BsaJI CCNNGG 3 cut(s) 128, 449, 613
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 648, 678
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 3 cut(s) 134, 271, 384
Bse1I ACTGG 2 cut(s) 303, 625
Bse3DI GCAATG 2 cut(s) 481, 667
BseBI CCWGG 3 cut(s) 286, 294, 326
BseDI CCNNGG 3 cut(s) 128, 449, 613
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 2 cut(s) 481, 667
BseNI ACTGG 2 cut(s) 303, 625
BseRI GAGGAG 2 cut(s) 32, 113
BseSI GKGCMC 2 cut(s) 76, 85
BseXI GCAGC 4 cut(s) 211, 276, 316, 351
BseYI CCCAGC 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 7 cut(s) 74, 134, 284, 388, 487, 618, 668
BshNI GGYRCC 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 5 cut(s) 135, 272, 385, 654, 669
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 21
BsmI GAATGC 2 cut(s) 109, 427
BsnI GGCC 7 cut(s) 74, 134, 284, 388, 487, 618, 668
BsoBI CYCGRG 1 cut(s) 29
Bsp120I GGGCCC 1 cut(s) 72
Bsp1286I GDGCHC 4 cut(s) 36, 76, 85, 380
Bsp1407I TGTACA 2 cut(s) 51, 570
Bsp143I GATC 3 cut(s) 25, 314, 442
Bsp19I CCATGG 1 cut(s) 613
BspACI CCGC 9 cut(s) 5, 66, 107, 113, 119, 122, 143, 275, 281
BspANI GGCC 7 cut(s) 74, 134, 284, 388, 487, 618, 668
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 3 cut(s) 73, 74, 126
BspMAI CTGCAG 2 cut(s) 310, 394
BspPI GGATC 2 cut(s) 322, 437
BspT107I GGYRCC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 481, 667
BsrFI RCCGGY 3 cut(s) 134, 271, 384
BsrGI TGTACA 2 cut(s) 51, 570
BsrI ACTGG 2 cut(s) 303, 625
BssAI RCCGGY 3 cut(s) 134, 271, 384
BssECI CCNNGG 3 cut(s) 128, 449, 613
BssMI GATC 3 cut(s) 25, 314, 442
BssNI GRCGYC 2 cut(s) 125, 322
BssT1I CCWWGG 2 cut(s) 449, 613
Bst2UI CCWGG 3 cut(s) 286, 294, 326
Bst4CI ACNGT 3 cut(s) 51, 346, 694
Bst6I CTCTTC 1 cut(s) 545
BstACI GRCGYC 2 cut(s) 125, 322
BstAUI TGTACA 2 cut(s) 51, 570
BstC8I GCNNGC 5 cut(s) 85, 136, 229, 273, 390
BstDEI CTNAG 1 cut(s) 209
BstDSI CCRYGG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 3 cut(s) 128, 272, 499
BstHHI GCGC 4 cut(s) 89, 127, 271, 498
BstKTI GATC 3 cut(s) 28, 317, 445
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 3 cut(s) 25, 314, 442
BstMWI GCNNNNNNNGC 6 cut(s) 80, 119, 131, 140, 281, 493
BstNI CCWGG 3 cut(s) 286, 294, 326
BstSCI CCNGG 3 cut(s) 284, 292, 324
BstSFI CTRYAG 4 cut(s) 306, 366, 390, 499
BstSLI GKGCMC 2 cut(s) 76, 85
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 211, 276, 316, 351
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 7 cut(s) 74, 134, 284, 388, 487, 618, 668
BtgI CCRYGG 1 cut(s) 613
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 3 cut(s) 355, 399, 690
Cac8I GCNNGC 5 cut(s) 85, 136, 229, 273, 390
CfoI GCGC 4 cut(s) 89, 127, 271, 498
Cfr10I RCCGGY 3 cut(s) 134, 271, 384
Cfr13I GGNCC 3 cut(s) 72, 73, 132
CseI GACGC 1 cut(s) 330
Csp6I GTAC 3 cut(s) 22, 52, 571
CviAII CATG 2 cut(s) 524, 614
CviQI GTAC 3 cut(s) 22, 52, 571
DdeI CTNAG 1 cut(s) 209
DinI GGCGCC 1 cut(s) 126
DpnI GATC 3 cut(s) 27, 316, 444
DpnII GATC 3 cut(s) 25, 314, 442
DraIII CACNNNGTG 1 cut(s) 44
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 2 cut(s) 449, 613
Eco24I GRGCYC 3 cut(s) 36, 76, 380
Eco53kI GAGCTC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 3 cut(s) 284, 292, 324
EcoT14I CCWWGG 2 cut(s) 449, 613
EcoT38I GRGCYC 3 cut(s) 36, 76, 380
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 2 cut(s) 449, 613
FaeI CATG 2 cut(s) 527, 617
FaiI YATR 7 cut(s) 362, 468, 501, 518, 525, 604, 615
FaqI GGGAC 1 cut(s) 21
FatI CATG 2 cut(s) 523, 613
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 8 cut(s) 120, 123, 144, 225, 282, 290, 330, 340
FriOI GRGCYC 3 cut(s) 36, 76, 380
Fsp4HI GCNGC 8 cut(s) 120, 123, 144, 225, 282, 290, 330, 340
GlaI GCGC 4 cut(s) 88, 126, 270, 497
GluI GCNGC 8 cut(s) 120, 123, 144, 225, 282, 290, 330, 340
GsaI CCCAGC 1 cut(s) 72
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 3 cut(s) 128, 272, 499
HaeIII GGCC 7 cut(s) 74, 134, 284, 388, 487, 618, 668
HapII CCGG 5 cut(s) 135, 272, 385, 654, 669
HgaI GACGC 1 cut(s) 330
HhaI GCGC 4 cut(s) 89, 127, 271, 498
Hin1I GRCGYC 2 cut(s) 125, 322
Hin1II CATG 2 cut(s) 527, 617
Hin6I GCGC 4 cut(s) 87, 125, 269, 496
HinP1I GCGC 4 cut(s) 87, 125, 269, 496
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 1 cut(s) 229
HinfI GANTC 2 cut(s) 462, 597
HpaII CCGG 5 cut(s) 135, 272, 385, 654, 669
HphI GGTGA 2 cut(s) 229, 365
Hpy166II GTNNAC 3 cut(s) 47, 366, 646
Hpy188I TCNGA 2 cut(s) 97, 249
Hpy188III TCNNGA 1 cut(s) 318
Hpy8I GTNNAC 3 cut(s) 47, 366, 646
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 3 cut(s) 51, 346, 694
HpyCH4V TGCA 5 cut(s) 227, 308, 332, 392, 482
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 119, 131, 140, 281, 493
HpyF3I CTNAG 1 cut(s) 209
Hsp92I GRCGYC 2 cut(s) 125, 322
Hsp92II CATG 2 cut(s) 527, 617
HspAI GCGC 4 cut(s) 87, 125, 269, 496
KasI GGCGCC 1 cut(s) 124
KroI GCCGGC 2 cut(s) 134, 271
KroNI GCCGGC 2 cut(s) 136, 273
Kzo9I GATC 3 cut(s) 25, 314, 442
LmnI GCTCC 3 cut(s) 266, 375, 383
Lsp1109I GCAGC 4 cut(s) 211, 276, 316, 351
MaeIII GTNAC 1 cut(s) 394
MalI GATC 3 cut(s) 27, 316, 444
MboI GATC 3 cut(s) 25, 314, 442
MboII GAAGA 1 cut(s) 562
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 4 cut(s) 36, 76, 85, 380
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 2 cut(s) 75, 405
MreI CGCCGGCG 1 cut(s) 271
MroNI GCCGGC 2 cut(s) 134, 271
MslI CAYNNNNRTG 1 cut(s) 515
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 5 cut(s) 135, 272, 385, 654, 669
MspR9I CCNGG 3 cut(s) 286, 294, 326
Mva1269I GAATGC 2 cut(s) 109, 427
MvaI CCWGG 3 cut(s) 286, 294, 326
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 6 cut(s) 80, 119, 131, 140, 281, 493
NaeI GCCGGC 2 cut(s) 136, 273
NarI GGCGCC 1 cut(s) 125
NcoI CCATGG 1 cut(s) 613
NdeII GATC 3 cut(s) 25, 314, 442
NgoMIV GCCGGC 2 cut(s) 134, 271
NlaIII CATG 2 cut(s) 527, 617
NlaIV GGNNCC 3 cut(s) 73, 74, 126
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 394
PctI GAATGC 2 cut(s) 109, 427
PdiI GCCGGC 2 cut(s) 136, 273
PfeI GAWTC 1 cut(s) 597
PkrI GCNGC 8 cut(s) 121, 124, 145, 226, 283, 291, 331, 341
PleI GAGTC 1 cut(s) 456
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 456
Psp124BI GAGCTC 1 cut(s) 380
Psp6I CCWGG 3 cut(s) 284, 292, 324
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 3 cut(s) 284, 292, 324
PspN4I GGNNCC 3 cut(s) 73, 74, 126
PspOMI GGGCCC 1 cut(s) 72
PspPI GGNCC 3 cut(s) 72, 73, 132
PstI CTGCAG 2 cut(s) 310, 394
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 3 cut(s) 23, 53, 572
RsaNI GTAC 3 cut(s) 22, 52, 571
RseI CAYNNNNRTG 1 cut(s) 515
SacI GAGCTC 1 cut(s) 380
SatI GCNGC 8 cut(s) 120, 123, 144, 225, 282, 290, 330, 340
Sau3AI GATC 3 cut(s) 25, 314, 442
Sau96I GGNCC 3 cut(s) 72, 73, 132
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 3 cut(s) 286, 294, 326
SduI GDGCHC 4 cut(s) 36, 76, 85, 380
SetI ASST 7 cut(s) 208, 233, 240, 360, 380, 404, 415
SfcI CTRYAG 4 cut(s) 306, 366, 390, 499
SfoI GGCGCC 1 cut(s) 126
SgrAI CRCCGGYG 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 515
SsiI CCGC 9 cut(s) 5, 66, 107, 113, 119, 122, 143, 275, 281
SspDI GGCGCC 1 cut(s) 124
SstI GAGCTC 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 284, 292, 324
StyI CCWWGG 2 cut(s) 449, 613
TaaI ACNGT 3 cut(s) 51, 346, 694
TaqI TCGA 1 cut(s) 558
TatI WGTACW 2 cut(s) 51, 570
TauI GCSGC 4 cut(s) 122, 125, 146, 284
TfiI GAWTC 1 cut(s) 597
TscAI CASTG 3 cut(s) 355, 399, 697
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 4 cut(s) 224, 289, 329, 339
Tsp45I GTSAC 1 cut(s) 394
TspGWI ACGGA 2 cut(s) 173, 279
TspRI CASTG 3 cut(s) 355, 399, 697
XmiI GTMKAC 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.