AT1G74900

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015351
Physical Location & Seq
Forward (+)
28133933 .. 28135667
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74900.1

Sequence Viewer

Length: 1362 bp
ATGAAACGTCTTTTTAGCAAATCGCTATGTACTTCGGCCGCCGGAGCCAATCTCAAACCACCGCCGGCAGATTCCGCCGCCATAGCTAAGCTTATCCTCTCTTCTCCGAACACAACTCACCAAGACGATCAATTTCTACTCTCCACCAAAACAACCCCATGGACCCCAAATCTCGTGAACTCTGTTCTCAAGCGACTCTGGAACCACGGCCCTAAAGCTCTCCAATTCTTCCACTTCCTCGACAATCACCACCGCGAATATGTCCACGACGCTTCCTCCTTCGATCTCGCAATCGACATTGCCGCCCGTCTTCACCTCCACCCCACCGTCTGGTCACTCATTCACCGTATGCGCTCTCTCCGCATCGGTCCTTCTCCCAAAACCTTCGCAATCGTCGCCGAGAGGTACGCATCCGCTGGGAAGCCCGATAAAGCGGTTAAGCTGTTTCTGAATATGCACGAACATGGTTGTTTTCAAGATTTAGCTTCTTTCAATACGATTCTCGATGTGTTGTGTAAATCTAAACGCGTAGAGAAAGCTTATGAGTTGTTTAGAGCTCTTAGGGGAAGATTTAGTGTTGATACAGTTACTTACAATGTGATTCTTAACGGTTGGTGTTTGATTAAAAGGACGCCTAAGGCTTTAGAAGTTTTGAAAGAGATGGTTGAGAGAGGGATAAACCCTAATTTGACTACTTATAACACAATGCTCAAAGGTTTTTTCAGAGCTGGGCAGATTAGACATGCTTGGGAATTCTTCTTGGAGATGAAGAAACGGGATTGTGAAATAGATGTTGTCACTTATACTACTGTGGTTCACGGGTTTGGTGTTGCGGGGGAGATAAAGCGGGCGAGGAATGTTTTCGATGAGATGATTAGAGAAGGTGTGCTTCCTTCTGTTGCGACGTACAATGCTATGATTCAAGTTTTGTGTAAGAAAGATAATGTGGAGAATGCGGTTGTGATGTTTGAGGAAATGGTGAGGAGAGGTTATGAGCCGAATGTGACGACTTATAATGTGTTGATAAGAGGGTTGTTTCACGCTGGAGAGTTTAGTCGTGGAGAGGAGTTGATGCAGAGAATGGAGAATGAAGGCTGTGAACCAAACTTCCAGACATATAACATGATGATCCGTTATTATTCGGAATGCAGCGAGGTCGAGAAAGCGTTGGGTTTGTTTGAGAAAATGGGGAGTGGTGATTGCTTGCCAAATCTAGATACTTACAACATACTGATAAGTGGAATGTTTGTGAGAAAAAGATCAGAAGATATGGTGGTCGCTGGGAATCAAGCTTTTGCAAAGGAGATCTTGAGATTGCAGAGTAAATCTGGTAGCCGGCTTCTCCGGAAGTTCAGGCTATGA

Protein Analysis

453

Amino Acids

51.72

Weight (kDa)

9.24

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 100 - 220 5.9e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 136 - 173 6.4e-06 PPR repeat family
PPR_1 PF12854 156 - 185 3.9e-06 PPR repeat
TPR_24 PF23276 181 - 290 1.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 191 - 238 1.6e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 194 - 241 6.3e-15 PPR repeat family
PPR_1 PF12854 224 - 257 1.2e-08 PPR repeat
PPR_2 PF13041 228 - 275 6.5e-17 PPR repeat family
PPR PF01535 232 - 261 1.4e-07 PPR repeat
PPR_3 PF13812 254 - 310 1.3e-12 Pentatricopeptide repeat domain
TPR_24 PF23276 255 - 353 8.5e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 260 - 291 2e-08 PPR repeat
PPR_2 PF13041 264 - 312 1.3e-13 PPR repeat family
PPR PF01535 266 - 296 2.1e-06 PPR repeat
PPR_long PF17177 280 - 412 1.3e-13 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 295 - 327 1.8e-09 PPR repeat
PPR_2 PF13041 298 - 346 1.5e-18 PPR repeat family
PPR PF01535 302 - 330 4e-08 PPR repeat
PPR_3 PF13812 322 - 380 4.3e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 329 - 362 6.7e-06 PPR repeat
PPR_2 PF13041 333 - 378 2.9e-12 PPR repeat family
PPR_2 PF13041 372 - 415 2.3e-12 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74900
fragaria_vesca FvH4_5g16320
malus_domestica MD06G1181800.v1.1 MD14G1187700.v1.1
prunus_persica Prupe.5G180100_v2.0.a1
pyrus_communis pycom06g16080 pycom14g15560
rosa_chinensis RchiOBHm_Chr7g0179811
rosa_laevigata RLG00000005322
rosa_multiflora Rmu_sc0001754.1_g000006 Rmu_sc0004156.1_g000009
rosa_roxburghii Rroxscaffold_3G00273450
rosa_rugosa Rorug06G0428500
rosa_samantha Rh7CG029100 Rh7DG028600
rosa_wichuraiana Rw7G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 699, 1014
AccB7I CCANNNNNTGG 1 cut(s) 330
AccII CGCG 2 cut(s) 255, 528
AccIII TCCGGA 1 cut(s) 1344
AclWI GGATC 1 cut(s) 1123
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 752
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 3 cut(s) 31, 407, 908
AfiI CCNNNNNNNGG 1 cut(s) 330
AflIII ACRYGT 1 cut(s) 526
AgsI TTSAA 4 cut(s) 476, 493, 655, 923
AloI GAACNNNNNNTCC 2 cut(s) 1092, 1124
AluBI AGCT 9 cut(s) 86, 91, 218, 442, 485, 539, 557, 728, 1292
AluI AGCT 9 cut(s) 86, 91, 218, 442, 485, 539, 557, 728, 1292
Alw21I GWGCWC 1 cut(s) 559
AlwI GGATC 1 cut(s) 1123
Aor13HI TCCGGA 1 cut(s) 1344
AoxI GGCC 2 cut(s) 36, 208
ApeKI GCWGC 1 cut(s) 1149
ApoI RAATTY 1 cut(s) 752
Asp700I GAANNNNTTC 1 cut(s) 860
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 3 cut(s) 162, 209, 368
AsuHPI GGTGA 6 cut(s) 110, 239, 305, 335, 991, 1208
AvaII GGWCC 2 cut(s) 162, 368
AxyI CCTNAGG 1 cut(s) 636
BanII GRGCYC 1 cut(s) 559
BauI CACGAG 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 1 cut(s) 1161
BccI CCATC 1 cut(s) 655
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 1138, 1172
BfaI CTAG 1 cut(s) 1214
BglII AGATCT 1 cut(s) 1305
BisI GCNGC 4 cut(s) 39, 78, 303, 1150
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 4 cut(s) 40, 79, 304, 1151
Bme18I GGWCC 2 cut(s) 162, 368
BmgT120I GGNCC 3 cut(s) 162, 209, 368
BmiI GGNNCC 3 cut(s) 46, 164, 203
BmsI GCATC 3 cut(s) 372, 419, 1062
BpiI GAAGAC 1 cut(s) 302
BplI GAGNNNNNCTC 2 cut(s) 36, 68
BpmI CTGGAG 1 cut(s) 1065
Bpu1102I GCTNAGC 1 cut(s) 87
BpuEI CTTGAG 2 cut(s) 173, 1330
BsaHI GRCGYC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 158, 205
BsaWI WCCGGW 1 cut(s) 1344
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 330
Bse118I RCCGGY 2 cut(s) 64, 1335
Bse21I CCTNAGG 1 cut(s) 636
Bse3DI GCAATG 1 cut(s) 297
BseAI TCCGGA 1 cut(s) 1344
BseDI CCNNGG 2 cut(s) 158, 205
BseGI GGATG 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 330
BseMI GCAATG 1 cut(s) 297
BseRI GAGGAG 2 cut(s) 997, 1079
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 1161
BseYI CCCAGC 3 cut(s) 416, 728, 1280
Bsh1236I CGCG 2 cut(s) 255, 528
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 38, 210
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 4 cut(s) 42, 65, 1336, 1345
BslI CCNNNNNNNGG 1 cut(s) 330
BsmI GAATGC 2 cut(s) 958, 1151
BsnI GGCC 2 cut(s) 38, 210
Bsp1286I GDGCHC 1 cut(s) 559
Bsp13I TCCGGA 1 cut(s) 1344
Bsp143I GATC 5 cut(s) 127, 283, 1128, 1259, 1305
Bsp1720I GCTNAGC 1 cut(s) 87
Bsp19I CCATGG 1 cut(s) 158
BspANI GGCC 2 cut(s) 38, 210
BspEI TCCGGA 1 cut(s) 1344
BspFNI CGCG 2 cut(s) 255, 528
BspLI GGNNCC 3 cut(s) 46, 164, 203
BspPI GGATC 1 cut(s) 1123
BsrDI GCAATG 1 cut(s) 297
BsrFI RCCGGY 2 cut(s) 64, 1335
BssAI RCCGGY 2 cut(s) 64, 1335
BssECI CCNNGG 2 cut(s) 158, 205
BssMI GATC 5 cut(s) 127, 283, 1128, 1259, 1305
BssNI GRCGYC 1 cut(s) 632
BssSI CACGAG 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 173
Bst4CI ACNGT 5 cut(s) 328, 347, 586, 611, 811
Bst6I CTCTTC 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 632
BstC8I GCNNGC 4 cut(s) 66, 849, 1205, 1337
BstDEI CTNAG 3 cut(s) 87, 560, 636
BstDSI CCRYGG 2 cut(s) 158, 205
BstF5I GGATG 1 cut(s) 410
BstFNI CGCG 2 cut(s) 255, 528
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 5 cut(s) 130, 286, 1131, 1262, 1308
BstMBI GATC 5 cut(s) 127, 283, 1128, 1259, 1305
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 5 cut(s) 44, 74, 83, 360, 395
BstNSI RCATGY 1 cut(s) 746
BstUI CGCG 2 cut(s) 255, 528
BstV1I GCAGC 1 cut(s) 1161
BstV2I GAAGAC 1 cut(s) 302
BstX2I RGATCY 1 cut(s) 1305
BstYI RGATCY 1 cut(s) 1305
BstZI CGGCCG 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 636
BsuRI GGCC 2 cut(s) 38, 210
BtgI CCRYGG 2 cut(s) 158, 205
BtsCI GGATG 1 cut(s) 410
Cac8I GCNNGC 4 cut(s) 66, 849, 1205, 1337
CfoI GCGC 1 cut(s) 354
Cfr10I RCCGGY 2 cut(s) 64, 1335
Cfr13I GGNCC 3 cut(s) 162, 209, 368
CseI GACGC 2 cut(s) 278, 640
Csp6I GTAC 3 cut(s) 30, 406, 907
CviAII CATG 4 cut(s) 159, 464, 743, 1123
CviQI GTAC 3 cut(s) 30, 406, 907
DdeI CTNAG 3 cut(s) 87, 560, 636
DpnI GATC 5 cut(s) 129, 285, 1130, 1261, 1307
DpnII GATC 5 cut(s) 127, 283, 1128, 1259, 1305
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 557
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 559
Eco47I GGWCC 2 cut(s) 162, 368
Eco52I CGGCCG 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 557
Eco81I CCTNAGG 1 cut(s) 636
EcoICRI GAGCTC 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 752
EcoT14I CCWWGG 1 cut(s) 158
EcoT38I GRGCYC 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 4 cut(s) 162, 467, 746, 1126
FalI AAGNNNNNCTT 4 cut(s) 873, 905, 1292, 1324
FatI CATG 4 cut(s) 158, 463, 742, 1122
FauI CCCGC 2 cut(s) 826, 840
Fnu4HI GCNGC 4 cut(s) 39, 78, 303, 1150
FokI GGATG 1 cut(s) 397
FriOI GRGCYC 1 cut(s) 559
Fsp4HI GCNGC 4 cut(s) 39, 78, 303, 1150
FspBI CTAG 1 cut(s) 1214
GlaI GCGC 1 cut(s) 353
GluI GCNGC 4 cut(s) 39, 78, 303, 1150
GsaI CCCAGC 3 cut(s) 420, 732, 1284
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 2 cut(s) 38, 210
HapII CCGG 4 cut(s) 42, 65, 1336, 1345
HgaI GACGC 2 cut(s) 278, 640
HhaI GCGC 1 cut(s) 354
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 4 cut(s) 162, 467, 746, 1126
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HindIII AAGCTT 3 cut(s) 89, 537, 1290
HinfI GANTC 6 cut(s) 71, 195, 499, 601, 919, 1285
HpaII CCGG 4 cut(s) 42, 65, 1336, 1345
HphI GGTGA 6 cut(s) 110, 239, 305, 335, 991, 1208
Hpy166II GTNNAC 4 cut(s) 178, 265, 817, 1100
Hpy188I TCNGA 5 cut(s) 108, 450, 725, 1144, 1264
Hpy188III TCNNGA 9 cut(s) 175, 199, 476, 503, 1111, 1159, 1214, 1309, 1345
Hpy8I GTNNAC 4 cut(s) 178, 265, 817, 1100
Hpy99I CGWCG 3 cut(s) 272, 398, 907
HpyAV CCTTC 6 cut(s) 289, 381, 394, 875, 903, 1085
HpyCH4III ACNGT 5 cut(s) 328, 347, 586, 611, 811
HpyCH4IV ACGT 2 cut(s) 7, 905
HpyCH4V TGCA 5 cut(s) 457, 1075, 1149, 1298, 1318
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 74, 83, 360, 395
HpyF3I CTNAG 3 cut(s) 87, 560, 636
HpySE526I ACGT 2 cut(s) 7, 905
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 4 cut(s) 162, 467, 746, 1126
HspAI GCGC 1 cut(s) 352
Kpn2I TCCGGA 1 cut(s) 1344
KroI GCCGGC 2 cut(s) 64, 1335
KroNI GCCGGC 2 cut(s) 66, 1337
Kzo9I GATC 5 cut(s) 127, 283, 1128, 1259, 1305
LmnI GCTCC 1 cut(s) 44
Lsp1109I GCAGC 1 cut(s) 1161
LweI GCATC 3 cut(s) 372, 419, 1062
MaeI CTAG 1 cut(s) 1214
MaeII ACGT 2 cut(s) 7, 905
MaeIII GTNAC 4 cut(s) 333, 586, 796, 1003
MalI GATC 5 cut(s) 129, 285, 1130, 1261, 1307
MboI GATC 5 cut(s) 127, 283, 1128, 1259, 1305
MboII GAAGA 7 cut(s) 93, 220, 302, 579, 748, 781, 1277
MflI RGATCY 1 cut(s) 1305
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 4 cut(s) 131, 224, 685, 752
MluI ACGCGT 1 cut(s) 526
MlyI GAGTC 1 cut(s) 189
MroI TCCGGA 1 cut(s) 1344
MroNI GCCGGC 2 cut(s) 64, 1335
MroXI GAANNNNTTC 1 cut(s) 860
MseI TTAA 3 cut(s) 438, 606, 624
MslI CAYNNNNRTG 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 4 cut(s) 42, 65, 1336, 1345
Mva1269I GAATGC 2 cut(s) 958, 1151
MvnI CGCG 2 cut(s) 255, 528
MwoI GCNNNNNNNGC 5 cut(s) 44, 74, 83, 360, 395
NaeI GCCGGC 2 cut(s) 66, 1337
NcoI CCATGG 1 cut(s) 158
NdeII GATC 5 cut(s) 127, 283, 1128, 1259, 1305
NgoMIV GCCGGC 2 cut(s) 64, 1335
NlaIII CATG 4 cut(s) 162, 467, 746, 1126
NlaIV GGNNCC 3 cut(s) 46, 164, 203
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 3 cut(s) 333, 796, 1003
NspI RCATGY 1 cut(s) 746
PctI GAATGC 2 cut(s) 958, 1151
PdiI GCCGGC 2 cut(s) 66, 1337
PdmI GAANNNNTTC 1 cut(s) 860
PfeI GAWTC 5 cut(s) 71, 499, 601, 919, 1285
PflMI CCANNNNNTGG 1 cut(s) 330
PkrI GCNGC 4 cut(s) 40, 79, 304, 1151
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PsiI TTATAA 2 cut(s) 699, 1014
Psp124BI GAGCTC 1 cut(s) 559
PspFI CCCAGC 3 cut(s) 416, 728, 1280
PspN4I GGNNCC 3 cut(s) 46, 164, 203
PspPI GGNCC 3 cut(s) 162, 209, 368
PsuI RGATCY 1 cut(s) 1305
RsaI GTAC 3 cut(s) 31, 407, 908
RsaNI GTAC 3 cut(s) 30, 406, 907
RseI CAYNNNNRTG 1 cut(s) 462
SacI GAGCTC 1 cut(s) 559
SaqAI TTAA 3 cut(s) 438, 606, 624
SatI GCNGC 4 cut(s) 39, 78, 303, 1150
Sau3AI GATC 5 cut(s) 127, 283, 1128, 1259, 1305
Sau96I GGNCC 3 cut(s) 162, 209, 368
SchI GAGTC 1 cut(s) 189
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 3 cut(s) 372, 419, 1062
SinI GGWCC 2 cut(s) 162, 368
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 188, 1309
SmoI CTYRAG 2 cut(s) 188, 1309
Sse9I AATT 4 cut(s) 131, 224, 685, 752
SspMI CTAG 1 cut(s) 1214
SstI GAGCTC 1 cut(s) 559
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 5 cut(s) 328, 347, 586, 611, 811
TaiI ACGT 2 cut(s) 10, 908
TaqI TCGA 6 cut(s) 240, 282, 294, 504, 864, 1158
TaqII GACCGA 1 cut(s) 356
TasI AATT 4 cut(s) 131, 224, 685, 752
TatI WGTACW 1 cut(s) 29
TauI GCSGC 3 cut(s) 41, 80, 305
TfiI GAWTC 5 cut(s) 71, 499, 601, 919, 1285
Tru1I TTAA 3 cut(s) 438, 606, 624
Tru9I TTAA 3 cut(s) 438, 606, 624
TseFI GTSAC 3 cut(s) 333, 796, 1003
TseI GCWGC 1 cut(s) 1149
Tsp45I GTSAC 3 cut(s) 333, 796, 1003
TspDTI ATGAA 3 cut(s) 17, 782, 1104
TspGWI ACGGA 1 cut(s) 1121
Van91I CCANNNNNTGG 1 cut(s) 330
VpaK11BI GGWCC 2 cut(s) 162, 368
XapI RAATTY 1 cut(s) 752
XbaI TCTAGA 1 cut(s) 1213
XceI RCATGY 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 860
XspI CTAG 1 cut(s) 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.