Rw7G002250

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015351
Physical Location & Seq
Reverse (-)
1867895 .. 1870449
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002250.1

Sequence Viewer

Length: 1467 bp
ATGTTCACTCTGCTTCGCAAAACCCCATCACTGAAAAACCCAGAACCCAGTTTCTCAATTCCACTACATCACAACCTAACCACCTCACCACCACAACCGCCTCAAGACGCCGCCTTTGCCACCCTTATCCTCAAATCCGACCCGCAATCTCTCCCCCGGATCCTCCACGACCCGAACATCCACTGGACCCCCGACTCCGTCAACAAAACCCTCAAACGCCTCTGGAACCATGGCCCCAAAGCCCTCGTCTTCTTCAAAACCCTCGACCGCCACCCCACCTACACCCACTCCTCCTCCTCCTACGACCACGCCGTCGACATCGCCGGCCGTTTACGCGACTACAAGTCCCTCTGGGCCTTCGTCGCCCGAATGCGAGCCCGCCGGGTCGGCCCCGGTCCGAGAACATTCGCCATCATCGCCGAGAGGTACGTCGCCGCCGGCAAACCCGATAGAGCTGTCAAAGTTTTTCTCTCTATGCATGAACATGGGTGCCCTCAGGATTTGAATTCTTTCAATACTGTTCTTGATGTGCTCTGTAAAGCTAAGCGAGTTGAAAAGGCTTACAATTTGTTTAAGGTGTTTCGGGGAAGATTTAGAGCTGATTGTGTTAGTTACAATGTGATTGTTAATGGGTGGTGTTTGATTAAGAGGACTCCTAAGGCATTAGAGGTTTTGAGGGAGATGGTTGAGAGAGGAATCGAGCCGAGTTTGGTTACTTATAATGTGATGCTTAAAGGGTATCTTAGAGCTGGGCAGGTTAAGGAGGCGTGGGAGTTTTTTTTGGAAATGAAGAAGAGGAAGTGTGAGATTGATGTTGTTACTTATACCACTCTGGTTCATGGGTTTGGTGTTGTCGGGGAGGTTAAGAAGGCGAGGAAGATTTTCAATGGGATGGTTGAGGAGGGGGTGCTGCCTTCGGTTGCGACTTACAATGCGTTGATTCAGGTTTTGTGTAAGAAGGATAGCGTGGAGAATGCAGTGGTGGTTTTTGAGGAGATGGTGAGTAAGGGTTATGTGCCTAATGTGACGACGTATAATGTGTTGATTAGAGGGCTGTGTCATGCTGGTGATATGGAGAGGGGGATGGAGTTGATGGGGAGGATGAAGGGTGATGAGTGTGAGCCGAATGTTCAGACGTATAATGTTGTGATTCGGTACTTTTGTGATGATGGGGAGATTGATAAGGCGTTGGATGTGTTTGAGAAGATGGGTAGGGGAGAGTGCTTGCCTAATTTGGATACATACAATGTGTTGATTAGTGCCATGTTTGTGAGGAAGAAACCTGAAGATTTGTTAGTGGCAGGGAAGTTGCTGATTGAGATGGTTGATAGAGGATTTTTGCCCCGCAAGTACACTTTTAATCGGGTTCTGGATGGGCTTTTGTTAACTGGTAATCAAGGTTTTGCAAAAGAGATTCTGAGATTGCAGAGCAAATGTGGCCGTCTTCCTCGTCAGGTGAAATTATAA

Protein Analysis

488

Amino Acids

55.46

Weight (kDa)

9.42

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 198 - 247 1.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 201 - 247 3.5e-12 PPR repeat family
PPR_3 PF13812 224 - 269 6.7e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 231 - 264 5.9e-08 PPR repeat
PPR_2 PF13041 235 - 282 5.9e-19 PPR repeat family
PPR PF01535 238 - 268 4.9e-09 PPR repeat
TPR_24 PF23276 256 - 364 2e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 261 - 317 5.2e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 267 - 299 2.3e-07 PPR repeat
PPR PF01535 273 - 303 5.7e-06 PPR repeat
PPR_long PF17177 287 - 418 9.2e-14 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 302 - 334 4.8e-09 PPR repeat
PPR_2 PF13041 305 - 354 2.8e-18 PPR repeat family
PPR PF01535 309 - 337 6.6e-07 PPR repeat
PPR_3 PF13812 329 - 387 4.7e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 336 - 369 3.4e-09 PPR repeat
PPR_2 PF13041 340 - 389 6.1e-15 PPR repeat family
PPR PF01535 344 - 373 5.2e-07 PPR repeat
PPR_1 PF12854 372 - 403 1.6e-09 PPR repeat
PPR_2 PF13041 375 - 422 1.3e-14 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015351)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74900
fragaria_vesca FvH4_5g16320
malus_domestica MD06G1181800.v1.1 MD14G1187700.v1.1
prunus_persica Prupe.5G180100_v2.0.a1
pyrus_communis pycom06g16080 pycom14g15560
rosa_chinensis RchiOBHm_Chr7g0179811
rosa_laevigata RLG00000005322
rosa_multiflora Rmu_sc0001754.1_g000006 Rmu_sc0004156.1_g000009
rosa_roxburghii Rroxscaffold_3G00273450
rosa_rugosa Rorug06G0428500
rosa_samantha Rh7CG029100 Rh7DG028600
rosa_wichuraiana Rw7G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 720, 1465
AasI GACNNNNNNGTC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 745
AccB1I GGYRCC 1 cut(s) 489
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 336
AciI CCGC 7 cut(s) 98, 111, 143, 268, 379, 435, 1345
AclWI GGATC 2 cut(s) 154, 167
AcoI YGGCCR 2 cut(s) 325, 1438
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 1305
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 3 cut(s) 428, 1157, 1352
AgsI TTSAA 5 cut(s) 256, 505, 514, 554, 886
AhdI GACNNNNNGTC 1 cut(s) 343
AluBI AGCT 4 cut(s) 455, 542, 599, 749
AluI AGCT 4 cut(s) 455, 542, 599, 749
Alw21I GWGCWC 1 cut(s) 534
AlwI GGATC 2 cut(s) 154, 167
AoxI GGCC 5 cut(s) 232, 325, 354, 388, 1438
ApeKI GCWGC 1 cut(s) 910
ApoI RAATTY 1 cut(s) 505
ArsI GACNNNNNNTTYG 4 cut(s) 98, 130, 231, 263
Asp700I GAANNNNTTC 2 cut(s) 509, 881
AspS9I GGNCC 5 cut(s) 186, 233, 354, 389, 395
AsuC2I CCSGG 3 cut(s) 157, 383, 393
AsuHPI GGTGA 4 cut(s) 78, 1012, 1079, 1121
AvaII GGWCC 2 cut(s) 186, 395
AxyI CCTNAGG 2 cut(s) 495, 657
BaeGI GKGCMC 1 cut(s) 494
BaeI ACNNNNGTAYC 2 cut(s) 1147, 1180
BamHI GGATCC 1 cut(s) 159
BanI GGYRCC 1 cut(s) 489
BanII GRGCYC 1 cut(s) 379
BbsI GAAGAC 2 cut(s) 241, 1436
Bbv12I GWGCWC 1 cut(s) 534
BbvI GCAGC 1 cut(s) 897
BceAI ACGGC 3 cut(s) 296, 312, 1425
BciVI GTATCC 1 cut(s) 1231
BcnI CCSGG 3 cut(s) 157, 383, 393
BfuAI ACCTGC 1 cut(s) 745
BfuI GTATCC 1 cut(s) 1231
BglI GCCNNNNNGGC 1 cut(s) 387
BisI GCNGC 3 cut(s) 111, 435, 911
BlpI GCTNAGC 1 cut(s) 543
BlsI GCNGC 3 cut(s) 112, 436, 912
Bme1390I CCNGG 3 cut(s) 157, 383, 393
Bme18I GGWCC 2 cut(s) 186, 395
BmeRI GACNNNNNGTC 1 cut(s) 343
BmgT120I GGNCC 5 cut(s) 186, 233, 354, 389, 395
BmiI GGNNCC 6 cut(s) 161, 188, 227, 235, 391, 491
BmrFI CCNGG 3 cut(s) 157, 383, 393
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 717
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 2 cut(s) 241, 1436
Bpu1102I GCTNAGC 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 87
BpuMI CCSGG 3 cut(s) 157, 383, 393
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 3 cut(s) 155, 229, 391
BsaXI ACNNNNNCTCC 4 cut(s) 272, 278, 302, 308
Bse118I RCCGGY 2 cut(s) 323, 437
Bse1I ACTGG 3 cut(s) 48, 188, 1393
Bse21I CCTNAGG 2 cut(s) 495, 657
BseDI CCNNGG 3 cut(s) 155, 229, 391
BseGI GGATG 6 cut(s) 177, 897, 1089, 1107, 1198, 1378
BseMII CTCAG 2 cut(s) 509, 1409
BseNI ACTGG 3 cut(s) 48, 188, 1393
BseRI GAGGAG 5 cut(s) 280, 283, 286, 914, 1007
BseSI GKGCMC 1 cut(s) 494
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 1 cut(s) 897
BseYI CCCAGC 1 cut(s) 749
Bsh1236I CGCG 1 cut(s) 336
Bsh1285I CGRYCG 2 cut(s) 268, 328
BshFI GGCC 5 cut(s) 234, 327, 356, 390, 1440
BshNI GGYRCC 1 cut(s) 489
BsiEI CGRYCG 2 cut(s) 268, 328
BsiHKAI GWGCWC 1 cut(s) 534
BsiSI CCGG 5 cut(s) 157, 324, 382, 393, 438
BslFI GGGAC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 2 cut(s) 375, 979
BsnI GGCC 5 cut(s) 234, 327, 356, 390, 1440
Bsp1286I GDGCHC 3 cut(s) 379, 494, 534
Bsp143I GATC 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 543
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 7 cut(s) 98, 111, 143, 268, 379, 435, 1345
BspANI GGCC 5 cut(s) 234, 327, 356, 390, 1440
BspCNI CTCAG 2 cut(s) 508, 1410
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 6 cut(s) 161, 188, 227, 235, 391, 491
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 2 cut(s) 154, 167
BspT107I GGYRCC 1 cut(s) 489
BsrFI RCCGGY 2 cut(s) 323, 437
BsrI ACTGG 3 cut(s) 48, 188, 1393
BssAI RCCGGY 2 cut(s) 323, 437
BssECI CCNNGG 3 cut(s) 155, 229, 391
BssMI GATC 1 cut(s) 159
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 229
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 1 cut(s) 788
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 5 cut(s) 325, 375, 379, 439, 1226
BstDEI CTNAG 5 cut(s) 495, 543, 657, 743, 1418
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 6 cut(s) 177, 897, 1089, 1107, 1198, 1378
BstFNI CGCG 1 cut(s) 336
BstKTI GATC 1 cut(s) 162
BstMBI GATC 1 cut(s) 159
BstMCI CGRYCG 2 cut(s) 268, 328
BstMWI GCNNNNNNNGC 6 cut(s) 116, 333, 362, 387, 416, 1437
BstSCI CCNGG 3 cut(s) 155, 381, 391
BstSLI GKGCMC 1 cut(s) 494
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 897
BstV2I GAAGAC 2 cut(s) 241, 1436
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BstZI CGGCCG 1 cut(s) 325
Bsu36I CCTNAGG 2 cut(s) 495, 657
BsuI GTATCC 1 cut(s) 1231
BsuRI GGCC 5 cut(s) 234, 327, 356, 390, 1440
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 2 cut(s) 304, 400
BtsCI GGATG 6 cut(s) 177, 897, 1089, 1107, 1198, 1378
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 3 cut(s) 29, 181, 984
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 5 cut(s) 325, 375, 379, 439, 1226
Cfr10I RCCGGY 2 cut(s) 323, 437
Cfr13I GGNCC 5 cut(s) 186, 233, 354, 389, 395
CpoI CGGWCCG 1 cut(s) 395
CseI GACGC 1 cut(s) 116
Csp6I GTAC 3 cut(s) 427, 1156, 1351
CspI CGGWCCG 1 cut(s) 395
CviAII CATG 6 cut(s) 230, 479, 485, 839, 1061, 1264
CviQI GTAC 3 cut(s) 427, 1156, 1351
DdeI CTNAG 5 cut(s) 495, 543, 657, 743, 1418
DpnI GATC 1 cut(s) 161
DpnII GATC 1 cut(s) 159
DrdI GACNNNNNNGTC 1 cut(s) 311
DriI GACNNNNNGTC 1 cut(s) 343
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 325, 1438
EagI CGGCCG 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 788
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 788
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 379
Eco47I GGWCC 2 cut(s) 186, 395
Eco52I CGGCCG 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 1305
Eco81I CCTNAGG 2 cut(s) 495, 657
EcoRI GAATTC 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 6 cut(s) 233, 482, 488, 842, 1064, 1267
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 331
FatI CATG 6 cut(s) 229, 478, 484, 838, 1060, 1263
FauI CCCGC 3 cut(s) 150, 386, 1352
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 3 cut(s) 111, 435, 911
FokI GGATG 6 cut(s) 164, 904, 1096, 1114, 1205, 1385
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 3 cut(s) 111, 435, 911
GluI GCNGC 3 cut(s) 111, 435, 911
GsaI CCCAGC 1 cut(s) 753
HaeIII GGCC 5 cut(s) 234, 327, 356, 390, 1440
HapII CCGG 5 cut(s) 157, 324, 382, 393, 438
HgaI GACGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 6 cut(s) 233, 482, 488, 842, 1064, 1267
HincII GTYRAC 3 cut(s) 202, 316, 1386
HindII GTYRAC 3 cut(s) 202, 316, 1386
HinfI GANTC 6 cut(s) 194, 652, 696, 940, 1150, 1414
HpaI GTTAAC 1 cut(s) 1386
HpaII CCGG 5 cut(s) 157, 324, 382, 393, 438
HphI GGTGA 4 cut(s) 78, 1012, 1079, 1121
Hpy166II GTNNAC 6 cut(s) 6, 202, 316, 332, 1353, 1386
Hpy188I TCNGA 4 cut(s) 139, 399, 1134, 1419
Hpy188III TCNNGA 5 cut(s) 104, 223, 497, 524, 1370
Hpy8I GTNNAC 6 cut(s) 6, 202, 316, 332, 1353, 1386
Hpy99I CGWCG 4 cut(s) 317, 365, 434, 1033
HpyAV CCTTC 5 cut(s) 367, 862, 924, 952, 1099
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4IV ACGT 3 cut(s) 429, 1031, 1136
HpyCH4V TGCA 4 cut(s) 478, 977, 1406, 1426
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 333, 362, 387, 416, 1437
HpyF3I CTNAG 5 cut(s) 495, 543, 657, 743, 1418
HpySE526I ACGT 3 cut(s) 429, 1031, 1136
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 6 cut(s) 233, 482, 488, 842, 1064, 1267
KroI GCCGGC 2 cut(s) 323, 437
KroNI GCCGGC 2 cut(s) 325, 439
KspAI GTTAAC 1 cut(s) 1386
Kzo9I GATC 1 cut(s) 159
Lsp1109I GCAGC 1 cut(s) 897
LweI GCATC 1 cut(s) 717
MaeII ACGT 3 cut(s) 429, 1031, 1136
MaeIII GTNAC 4 cut(s) 611, 712, 817, 1024
MalI GATC 1 cut(s) 161
MboI GATC 1 cut(s) 159
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 3 cut(s) 379, 494, 534
MluCI AATT 5 cut(s) 57, 505, 565, 1231, 1460
MlyI GAGTC 2 cut(s) 188, 646
MmeI TCCRAC 2 cut(s) 162, 1170
Mph1103I ATGCAT 1 cut(s) 480
MroNI GCCGGC 2 cut(s) 323, 437
MroXI GAANNNNTTC 2 cut(s) 509, 881
MseI TTAA 8 cut(s) 573, 627, 645, 732, 759, 864, 1359, 1385
MslI CAYNNNNRTG 3 cut(s) 483, 1065, 1268
MspI CCGG 5 cut(s) 157, 324, 382, 393, 438
MspR9I CCNGG 3 cut(s) 157, 383, 393
Mva1269I GAATGC 2 cut(s) 375, 979
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 6 cut(s) 116, 333, 362, 387, 416, 1437
NaeI GCCGGC 2 cut(s) 325, 439
NciI CCSGG 3 cut(s) 157, 383, 393
NcoI CCATGG 1 cut(s) 229
NdeII GATC 1 cut(s) 159
NgoMIV GCCGGC 2 cut(s) 323, 437
NlaIII CATG 6 cut(s) 233, 482, 488, 842, 1064, 1267
NlaIV GGNNCC 6 cut(s) 161, 188, 227, 235, 391, 491
NmeAIII GCCGAG 2 cut(s) 445, 729
NmuCI GTSAC 1 cut(s) 1024
NsiI ATGCAT 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 2 cut(s) 375, 979
PdiI GCCGGC 2 cut(s) 325, 439
PdmI GAANNNNTTC 2 cut(s) 509, 881
PfeI GAWTC 4 cut(s) 696, 940, 1150, 1414
PflFI GACNNNGTC 1 cut(s) 197
PkrI GCNGC 3 cut(s) 112, 436, 912
PleI GAGTC 2 cut(s) 188, 646
PpsI GAGTC 2 cut(s) 188, 646
PsiI TTATAA 2 cut(s) 720, 1465
PspFI CCCAGC 1 cut(s) 749
PspN4I GGNNCC 6 cut(s) 161, 188, 227, 235, 391, 491
PspPI GGNCC 5 cut(s) 186, 233, 354, 389, 395
PsuI RGATCY 1 cut(s) 159
PsyI GACNNNGTC 1 cut(s) 197
RsaI GTAC 3 cut(s) 428, 1157, 1352
RsaNI GTAC 3 cut(s) 427, 1156, 1351
RseI CAYNNNNRTG 3 cut(s) 483, 1065, 1268
Rsr2I CGGWCCG 1 cut(s) 395
RsrII CGGWCCG 1 cut(s) 395
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 8 cut(s) 573, 627, 645, 732, 759, 864, 1359, 1385
SatI GCNGC 3 cut(s) 111, 435, 911
Sau3AI GATC 1 cut(s) 159
Sau96I GGNCC 5 cut(s) 186, 233, 354, 389, 395
SchI GAGTC 2 cut(s) 188, 646
ScrFI CCNGG 3 cut(s) 157, 383, 393
SduI GDGCHC 3 cut(s) 379, 494, 534
SfaNI GCATC 1 cut(s) 717
SinI GGWCC 2 cut(s) 186, 395
SmiMI CAYNNNNRTG 3 cut(s) 483, 1065, 1268
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 5 cut(s) 57, 505, 565, 1231, 1460
SsiI CCGC 7 cut(s) 98, 111, 143, 268, 379, 435, 1345
StyD4I CCNGG 3 cut(s) 155, 381, 391
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 520
TaiI ACGT 3 cut(s) 432, 1034, 1139
TaqI TCGA 3 cut(s) 264, 315, 699
TasI AATT 5 cut(s) 57, 505, 565, 1231, 1460
TatI WGTACW 1 cut(s) 1350
TauI GCSGC 2 cut(s) 113, 437
TfiI GAWTC 4 cut(s) 696, 940, 1150, 1414
Tru1I TTAA 8 cut(s) 573, 627, 645, 732, 759, 864, 1359, 1385
Tru9I TTAA 8 cut(s) 573, 627, 645, 732, 759, 864, 1359, 1385
TscAI CASTG 3 cut(s) 36, 188, 984
TseFI GTSAC 1 cut(s) 1024
TseI GCWGC 1 cut(s) 910
Tsp45I GTSAC 1 cut(s) 1024
TspDTI ATGAA 4 cut(s) 495, 803, 827, 1118
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 3 cut(s) 36, 188, 984
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 2 cut(s) 186, 395
XapI RAATTY 1 cut(s) 505
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 2 cut(s) 509, 881
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.