Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0015351

Physical Location & Seq

Genomic Coordinates Reverse (-)

27977315 .. 27978157

Gene Structure 843 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1187700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 843 bp

ATGCTCGCTCAGCAACTCAAAACTGCCGCACCGAAAAACCCAAAACCCAGTTTTCTAATTCCGTGTCGGAGCTTCACCACCTCACCTTCTCATCCCTCTCAAGACTCTCACTTGGCGAACCTGATTCTCAAATCCTACCCGCAAACCCTAACCCAGATTCTTCACAGCCCCCAAATCGATTGGACTTCCGATTTGGTCGACAAAACCCTGAAGAGGCTCTGGAATCATGGACCCAAAGCCCTCCAGTTCTTCAGAATACTCGACCACCATCCGAACTACACACACTCTTGCTCCTCCTTCGACCACGCCGTCGATATTGCCGGCCGCCTGCGCGACTACAAGTCCCTCTGGACCCTCGTGGCCCGAATGAGGGCCCGCAGGCTCGGCCCTGGGCCGAGGACTTTCGCTATCATCACGGAGAGGTATGTGGCTGCTGGTAAGCCTGATAGAGCCGTTAAGGTGTTCCTGTCAATGCATGAACATGGTTGTCCTCAGGATTTGAATTCGTTTAATACAATTCTTGATGTGCTTTGTAAAGCTAAGCGTGTCGAAAAGGCGTATAATTTGTTTAAGGTGTTTAGGGGCAGGTTTAAGGCCGATTGTGTTAGTTACAATATAATTGCGAACGGGTGGTGTTTGATTAAGCGAACCCCGAAAGCTTTGGAGCTTTTGGGGGAGATGGTGGAGAGGGGATTGGACCCGAGTTTAACGACGTTTAACATAATGCTTAAAGGATATTTTAGAGCTGGTCAGATTAAGGAAGCTTGGGAGTTCTTTTTGCAAATGAAGAAGAGGAAGGTTTTGATGAGATGGTTGGGAAGGTGTTTGGTTACAATGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

281

Amino Acids

32.21

Weight (kDa)

10.0

Isoelectric Point (pI)

38.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_1	PF12854	162 - 191	9.5e-06	PPR repeat
PPR_2	PF13041	200 - 247	2.7e-10	PPR repeat family
PPR_1	PF12854	230 - 263	7.2e-08	PPR repeat
PPR_2	PF13041	234 - 267	3.3e-09	PPR repeat family
PPR	PF01535	238 - 266	1.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74900
fragaria_vesca	FvH4_5g16320
malus_domestica	MD06G1181800.v1.1 MD14G1187700.v1.1
prunus_persica	Prupe.5G180100_v2.0.a1
pyrus_communis	pycom06g16080 pycom14g15560
rosa_chinensis	RchiOBHm_Chr7g0179811
rosa_laevigata	RLG00000005322
rosa_multiflora	Rmu_sc0001754.1_g000006 Rmu_sc0004156.1_g000009
rosa_roxburghii	Rroxscaffold_3G00273450
rosa_rugosa	Rorug06G0428500
rosa_samantha	Rh7CG029100 Rh7DG028600
rosa_wichuraiana	Rw7G002250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	308
Acc36I	ACCTGC	1 cut(s)	576
AccI	GTMKAC	1 cut(s)	198
AccII	CGCG	1 cut(s)	333
AciI	CCGC	4 cut(s)	27, 140, 325, 376
AcoI	YGGCCR	1 cut(s)	322
AcsI	RAATTY	1 cut(s)	502
AcuI	CTGAAG	2 cut(s)	230, 235
AfiI	CCNNNNNNNGG	3 cut(s)	213, 369, 370
AgsI	TTSAA	1 cut(s)	502
AhdI	GACNNNNNGTC	1 cut(s)	340
AjnI	CCWGG	1 cut(s)	388
AluBI	AGCT	6 cut(s)	72, 539, 659, 667, 746, 764
AluI	AGCT	6 cut(s)	72, 539, 659, 667, 746, 764
Ama87I	CYCGRG	1 cut(s)	700
AoxI	GGCC	6 cut(s)	322, 360, 372, 385, 392, 594
ApaI	GGGCCC	1 cut(s)	376
ApeKI	GCWGC	1 cut(s)	431
ApoI	RAATTY	1 cut(s)	502
ArsI	GACNNNNNNTTYG	2 cut(s)	175, 207
AspLEI	GCGC	1 cut(s)	333
AspS9I	GGNCC	8 cut(s)	230, 351, 361, 372, 373, 386, 392, 697
AsuHPI	GGTGA	2 cut(s)	67, 75
AvaI	CYCGRG	1 cut(s)	700
AvaII	GGWCC	3 cut(s)	230, 351, 697
AxyI	CCTNAGG	1 cut(s)	492
BaeGI	GKGCMC	1 cut(s)	376
BanII	GRGCYC	1 cut(s)	376
BauI	CACGAG	1 cut(s)	356
BbvI	GCAGC	1 cut(s)	418
BccI	CCATC	3 cut(s)	276, 673, 804
BceAI	ACGGC	2 cut(s)	293, 437
BciT130I	CCWGG	1 cut(s)	390
BfuAI	ACCTGC	1 cut(s)	576
BisI	GCNGC	3 cut(s)	27, 325, 432
BlpI	GCTNAGC	2 cut(s)	9, 540
BlsI	GCNGC	3 cut(s)	28, 326, 433
Bme1390I	CCNGG	1 cut(s)	390
Bme18I	GGWCC	3 cut(s)	230, 351, 697
BmeRI	GACNNNNNGTC	1 cut(s)	340
BmeT110I	CYCGRG	1 cut(s)	700
BmgT120I	GGNCC	8 cut(s)	230, 351, 361, 372, 373, 386, 392, 697
BmiI	GGNNCC	4 cut(s)	232, 353, 374, 699
BmrFI	CCNGG	1 cut(s)	390
BmrI	ACTGGG	1 cut(s)	42
BmuI	ACTGGG	1 cut(s)	42
BpmI	CTGGAG	1 cut(s)	227
Bpu1102I	GCTNAGC	2 cut(s)	9, 540
BpuEI	CTTGAG	1 cut(s)	84
Bsa29I	ATCGAT	1 cut(s)	177
BsaJI	CCNNGG	3 cut(s)	388, 389, 395
BsaXI	ACNNNNNCTCC	2 cut(s)	275, 305
Bsc4I	CCNNNNNNNGG	3 cut(s)	213, 369, 370
Bse118I	RCCGGY	1 cut(s)	320
Bse1I	ACTGG	2 cut(s)	48, 244
Bse21I	CCTNAGG	1 cut(s)	492
BseBI	CCWGG	1 cut(s)	390
BseCI	ATCGAT	1 cut(s)	177
BseDI	CCNNGG	3 cut(s)	388, 389, 395
BseGI	GGATG	2 cut(s)	91, 268
BseLI	CCNNNNNNNGG	3 cut(s)	213, 369, 370
BseMII	CTCAG	2 cut(s)	23, 506
BseNI	ACTGG	2 cut(s)	48, 244
BseRI	GAGGAG	1 cut(s)	283
BseSI	GKGCMC	1 cut(s)	376
BseX3I	CGGCCG	1 cut(s)	322
BseXI	GCAGC	1 cut(s)	418
Bsh1236I	CGCG	1 cut(s)	333
Bsh1285I	CGRYCG	1 cut(s)	325
BshFI	GGCC	6 cut(s)	324, 362, 374, 387, 394, 596
BshVI	ATCGAT	1 cut(s)	177
BsiEI	CGRYCG	1 cut(s)	325
BsiHKCI	CYCGRG	1 cut(s)	700
BsiSI	CCGG	1 cut(s)	321
BslFI	GGGAC	1 cut(s)	328
BslI	CCNNNNNNNGG	3 cut(s)	213, 369, 370
BsmFI	GGGAC	1 cut(s)	328
BsnI	GGCC	6 cut(s)	324, 362, 374, 387, 394, 596
BsoBI	CYCGRG	1 cut(s)	700
Bsp120I	GGGCCC	1 cut(s)	372
Bsp1286I	GDGCHC	1 cut(s)	376
Bsp1720I	GCTNAGC	2 cut(s)	9, 540
BspACI	CCGC	4 cut(s)	27, 140, 325, 376
BspANI	GGCC	6 cut(s)	324, 362, 374, 387, 394, 596
BspCNI	CTCAG	2 cut(s)	22, 505
BspDI	ATCGAT	1 cut(s)	177
BspFNI	CGCG	1 cut(s)	333
BspLI	GGNNCC	4 cut(s)	232, 353, 374, 699
BspMI	ACCTGC	1 cut(s)	576
BsrFI	RCCGGY	1 cut(s)	320
BsrI	ACTGG	2 cut(s)	48, 244
BssAI	RCCGGY	1 cut(s)	320
BssECI	CCNNGG	3 cut(s)	388, 389, 395
BssSI	CACGAG	1 cut(s)	356
Bst2BI	CACGAG	1 cut(s)	356
Bst2UI	CCWGG	1 cut(s)	390
Bst6I	CTCTTC	2 cut(s)	206, 785
BstC8I	GCNNGC	5 cut(s)	6, 322, 329, 376, 380
BstDEI	CTNAG	3 cut(s)	9, 492, 540
BstF5I	GGATG	2 cut(s)	91, 268
BstFNI	CGCG	1 cut(s)	333
BstHHI	GCGC	1 cut(s)	333
BstMCI	CGRYCG	1 cut(s)	325
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 330, 384
BstNI	CCWGG	1 cut(s)	390
BstSCI	CCNGG	1 cut(s)	388
BstSLI	GKGCMC	1 cut(s)	376
BstUI	CGCG	1 cut(s)	333
BstV1I	GCAGC	1 cut(s)	418
BstZI	CGGCCG	1 cut(s)	322
Bsu15I	ATCGAT	1 cut(s)	177
Bsu36I	CCTNAGG	1 cut(s)	492
BsuRI	GGCC	6 cut(s)	324, 362, 374, 387, 394, 596
BsuTUI	ATCGAT	1 cut(s)	177
BtsCI	GGATG	2 cut(s)	91, 268
BveI	ACCTGC	1 cut(s)	576
Cac8I	GCNNGC	5 cut(s)	6, 322, 329, 376, 380
CfoI	GCGC	1 cut(s)	333
Cfr10I	RCCGGY	1 cut(s)	320
Cfr13I	GGNCC	8 cut(s)	230, 351, 361, 372, 373, 386, 392, 697
ClaI	ATCGAT	1 cut(s)	177
CviAII	CATG	3 cut(s)	227, 476, 482
DdeI	CTNAG	3 cut(s)	9, 492, 540
DrdI	GACNNNNNNGTC	1 cut(s)	308
DriI	GACNNNNNGTC	1 cut(s)	340
DseDI	GACNNNNNNGTC	1 cut(s)	308
EaeI	YGGCCR	1 cut(s)	322
EagI	CGGCCG	1 cut(s)	322
Eam1104I	CTCTTC	2 cut(s)	206, 785
Eam1105I	GACNNNNNGTC	1 cut(s)	340
EarI	CTCTTC	2 cut(s)	206, 785
EclXI	CGGCCG	1 cut(s)	322
Eco24I	GRGCYC	1 cut(s)	376
Eco47I	GGWCC	3 cut(s)	230, 351, 697
Eco52I	CGGCCG	1 cut(s)	322
Eco57I	CTGAAG	2 cut(s)	230, 235
Eco81I	CCTNAGG	1 cut(s)	492
Eco88I	CYCGRG	1 cut(s)	700
EcoO109I	RGGNCCY	1 cut(s)	372
EcoRI	GAATTC	1 cut(s)	502
EcoRII	CCWGG	1 cut(s)	388
EcoT22I	ATGCAT	1 cut(s)	477
EcoT38I	GRGCYC	1 cut(s)	376
FaeI	CATG	3 cut(s)	230, 479, 485
FaiI	YATR	7 cut(s)	228, 426, 477, 483, 561, 617, 722
FaqI	GGGAC	1 cut(s)	328
FatI	CATG	3 cut(s)	226, 475, 481
FauI	CCCGC	2 cut(s)	147, 383
FblI	GTMKAC	1 cut(s)	198
Fnu4HI	GCNGC	3 cut(s)	27, 325, 432
FokI	GGATG	2 cut(s)	78, 255
FriOI	GRGCYC	1 cut(s)	376
Fsp4HI	GCNGC	3 cut(s)	27, 325, 432
GlaI	GCGC	1 cut(s)	332
GluI	GCNGC	3 cut(s)	27, 325, 432
GsuI	CTGGAG	1 cut(s)	227
HaeIII	GGCC	6 cut(s)	324, 362, 374, 387, 394, 596
HapII	CCGG	1 cut(s)	321
HhaI	GCGC	1 cut(s)	333
Hin1II	CATG	3 cut(s)	230, 479, 485
Hin6I	GCGC	1 cut(s)	331
HinP1I	GCGC	1 cut(s)	331
HincII	GTYRAC	1 cut(s)	199
HindII	GTYRAC	1 cut(s)	199
HindIII	AAGCTT	2 cut(s)	657, 762
HinfI	GANTC	4 cut(s)	104, 124, 157, 223
HpaII	CCGG	1 cut(s)	321
HphI	GGTGA	2 cut(s)	67, 75
Hpy166II	GTNNAC	1 cut(s)	199
Hpy188I	TCNGA	5 cut(s)	69, 190, 254, 273, 753
Hpy188III	TCNNGA	5 cut(s)	101, 220, 349, 494, 521
Hpy8I	GTNNAC	1 cut(s)	199
Hpy99I	CGWCG	2 cut(s)	314, 715
HpyAV	CCTTC	4 cut(s)	96, 307, 790, 813
HpyCH4IV	ACGT	1 cut(s)	713
HpyCH4V	TGCA	2 cut(s)	475, 781
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 330, 384
HpyF3I	CTNAG	3 cut(s)	9, 492, 540
HpySE526I	ACGT	1 cut(s)	713
Hsp92II	CATG	3 cut(s)	230, 479, 485
HspAI	GCGC	1 cut(s)	331
KroI	GCCGGC	1 cut(s)	320
KroNI	GCCGGC	1 cut(s)	322
LmnI	GCTCC	3 cut(s)	69, 296, 664
Lsp1109I	GCAGC	1 cut(s)	418
MaeII	ACGT	1 cut(s)	713
MaeIII	GTNAC	2 cut(s)	608, 829
MboII	GAAGA	5 cut(s)	152, 223, 241, 799, 802
MhlI	GDGCHC	1 cut(s)	376
MluCI	AATT	5 cut(s)	57, 502, 516, 562, 618
MlyI	GAGTC	1 cut(s)	98
MmeI	TCCRAC	1 cut(s)	47
Mph1103I	ATGCAT	1 cut(s)	477
MroNI	GCCGGC	1 cut(s)	320
MseI	TTAA	9 cut(s)	456, 510, 570, 591, 642, 707, 717, 729, 756
MslI	CAYNNNNRTG	1 cut(s)	480
MspI	CCGG	1 cut(s)	321
MspR9I	CCNGG	1 cut(s)	390
MvaI	CCWGG	1 cut(s)	390
MvnI	CGCG	1 cut(s)	333
MwoI	GCNNNNNNNGC	3 cut(s)	10, 330, 384
NaeI	GCCGGC	1 cut(s)	322
NgoMIV	GCCGGC	1 cut(s)	320
NlaIII	CATG	3 cut(s)	230, 479, 485
NlaIV	GGNNCC	4 cut(s)	232, 353, 374, 699
NmeAIII	GCCGAG	2 cut(s)	363, 420
NsiI	ATGCAT	1 cut(s)	477
PasI	CCCWGGG	1 cut(s)	389
PcsI	WCGNNNNNNNCGW	1 cut(s)	306
PdiI	GCCGGC	1 cut(s)	322
PfeI	GAWTC	3 cut(s)	124, 157, 223
PkrI	GCNGC	3 cut(s)	28, 326, 433
PleI	GAGTC	1 cut(s)	98
PpsI	GAGTC	1 cut(s)	98
Psp6I	CCWGG	1 cut(s)	388
PspGI	CCWGG	1 cut(s)	388
PspN4I	GGNNCC	4 cut(s)	232, 353, 374, 699
PspOMI	GGGCCC	1 cut(s)	372
PspPI	GGNCC	8 cut(s)	230, 351, 361, 372, 373, 386, 392, 697
RseI	CAYNNNNRTG	1 cut(s)	480
SalI	GTCGAC	1 cut(s)	197
SaqAI	TTAA	9 cut(s)	456, 510, 570, 591, 642, 707, 717, 729, 756
SatI	GCNGC	3 cut(s)	27, 325, 432
Sau96I	GGNCC	8 cut(s)	230, 351, 361, 372, 373, 386, 392, 697
SchI	GAGTC	1 cut(s)	98
ScrFI	CCNGG	1 cut(s)	390
SduI	GDGCHC	1 cut(s)	376
SinI	GGWCC	3 cut(s)	230, 351, 697
SmiMI	CAYNNNNRTG	1 cut(s)	480
SmlI	CTYRAG	1 cut(s)	99
SmoI	CTYRAG	1 cut(s)	99
Sse9I	AATT	5 cut(s)	57, 502, 516, 562, 618
SsiI	CCGC	4 cut(s)	27, 140, 325, 376
StyD4I	CCNGG	1 cut(s)	388
TaiI	ACGT	1 cut(s)	716
TaqI	TCGA	6 cut(s)	177, 198, 261, 300, 312, 549
TasI	AATT	5 cut(s)	57, 502, 516, 562, 618
TauI	GCSGC	2 cut(s)	29, 327
TfiI	GAWTC	3 cut(s)	124, 157, 223
Tru1I	TTAA	9 cut(s)	456, 510, 570, 591, 642, 707, 717, 729, 756
Tru9I	TTAA	9 cut(s)	456, 510, 570, 591, 642, 707, 717, 729, 756
TseI	GCWGC	1 cut(s)	431
TspDTI	ATGAA	2 cut(s)	492, 800
TspGWI	ACGGA	2 cut(s)	51, 431
VpaK11BI	GGWCC	3 cut(s)	230, 351, 697
XapI	RAATTY	1 cut(s)	502
XmiI	GTMKAC	1 cut(s)	198
Zsp2I	ATGCAT	1 cut(s)	477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.