Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0015351

Physical Location & Seq

Genomic Coordinates Reverse (-)

32085909 .. 32087372

Gene Structure 1464 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1181800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGCTCACTCTGCAACTCAAAACTGCCGCACAGAAAAACCCAACACCCGGTTTTCCAATTCCGTGTCGGAGCCTCACCACCTCACCTTCACATCCCTCTCAAGACTCTCACTTGGCGAAACTGATTCTCAAATCCGACCCGCAAACCCTAACCCAGATTCTTCACAGCCCCGAAATCGACTGGTCTTCGGATTTGGTCGACAAAACCCTGAAGAGGCTCTGGAATCATGGACCCAAAGCCCTCCAATTCTTCAAAATCCTCGACCATCATCCGAACTACACGCATTCTTGCTCCTCCTTCGACCACGCAGTCGACGTTGCCGGCCGCCTGCGCGACTACAAGTCCCTCTGGACCCTCGTGGCCCGAATGCGGTCCCGTAGGCTCGGCCCCGGGCCGAGGACTTTCGCTATCATCACCGAGAGGTATGTGGCTGCTGGTAAGCCTGATAGAGCCGTTAAGGTGTTCCTGTCAATGCATGAACATGGTTGTCCTCAGGATTTGAATTCGTTTAATACAATTCTTGATGTGCTTTGTAAAGCTAAGCGTGTGGAAAAGGCGTATAATTTGTTTAAGGTGTTTAGGGGCAGGTTTAAGGCTGATCGTGTTAGTTACAATATAATTGCGAACGGGTGGTGTTTGATTAAGCGAACCCCGAAAGCTTTGGAGCTTTTGGGGGAGATGGTGGAGAGGGGGTTAGACCCGAGTTTAACGACGTTTAACATAATGCTTAAAGGGTATTTCAGAGCTGGTCAGATTAAGGAAGCTTGGGAGTTCTTTTTGCAGATGAAGAAGAGGAAGTGTGAGATTGATGTTGTTACTTACACTACTTTGGTTCATGGGTTTGGTGTTGTCGGGGAGATTAAGAAAGCTCGAAAGGTTTTCGATGAGATGGTTGGGGAAGGCGTGCTTCCTTCGGTGGCAACGTACAATGCTTTGATTCAGGTTTTGTGTAAGAAAGATAATGTGGAAAATGCTGTTGTGGTTTTTGAGGAAATGGTGAGCAAAGGTTATGTGCCTAATGTGACGACTTATAATGTATTGATTAGAGGATTGTGTCATGCGGGAAACATGGATAGGGCGCTGGCGTTGTTGGACAGAATGAAGGATGATGAGTGTGAGCCAAATGTTCAGACATATAATGTGGTGATCCGGTACTTTTGTGACGCCGGAGAGATTGAAAAGGCGTTGAATGAGTTTGAGAAGATGGGTCGTGGTGATTGCTTGCCTAATCTGGACACGTACAATGTTTTGATTAGTGCCATGTTTGTGAGGAAGAAACCTGAAGACCTGTTAGTGGCAGGGAAGTTGTTGATTGAGATGGTTGATAGAGGATTTTTGCCGCGAAGGTTCACCTTCAATCGCGTTCTGGATGGGCTTTTGGTAACAGGTAATCAAGGTTTTGCAAAAGAGATTTTAAGATTGCAGAGCAAATGTGGCCGTCTTCCCCGTCAAGTAAAATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

488

Amino Acids

55.27

Weight (kDa)

9.36

Isoelectric Point (pI)

35.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	200 - 247	5.5e-10	PPR repeat family
PPR_3	PF13812	223 - 268	1e-05	Pentatricopeptide repeat domain
TPR_24	PF23276	227 - 331	7.2e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	230 - 263	1.5e-07	PPR repeat
PPR_2	PF13041	238 - 281	1.9e-16	PPR repeat family
PPR	PF01535	238 - 267	2.1e-07	PPR repeat
TPR_24	PF23276	250 - 364	2.2e-09	Fungal tetratrico peptide repeats
PPR_long	PF17177	252 - 336	4.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	261 - 316	3.1e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	266 - 298	4.7e-09	PPR repeat
PPR_2	PF13041	270 - 310	4.6e-10	PPR repeat family
PPR	PF01535	272 - 302	9.5e-07	PPR repeat
PPR_long	PF17177	287 - 417	1.6e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	301 - 333	9.1e-10	PPR repeat
PPR_2	PF13041	304 - 353	8.2e-19	PPR repeat family
PPR	PF01535	308 - 336	1.7e-07	PPR repeat
TPR_24	PF23276	308 - 401	3e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	328 - 386	1.1e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	335 - 368	2.2e-12	PPR repeat
PPR_2	PF13041	339 - 388	3.9e-17	PPR repeat family
PPR	PF01535	343 - 372	2.9e-08	PPR repeat
PPR_1	PF12854	371 - 402	4.6e-07	PPR repeat
PPR_2	PF13041	374 - 421	4.1e-13	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0015351)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74900
fragaria_vesca	FvH4_5g16320
malus_domestica	MD06G1181800.v1.1 MD14G1187700.v1.1
prunus_persica	Prupe.5G180100_v2.0.a1
pyrus_communis	pycom06g16080 pycom14g15560
rosa_chinensis	RchiOBHm_Chr7g0179811
rosa_laevigata	RLG00000005322
rosa_multiflora	Rmu_sc0001754.1_g000006 Rmu_sc0004156.1_g000009
rosa_roxburghii	Rroxscaffold_3G00273450
rosa_rugosa	Rorug06G0428500
rosa_samantha	Rh7CG029100 Rh7DG028600
rosa_wichuraiana	Rw7G002250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1032
AasI	GACNNNNNNGTC	1 cut(s)	308
Acc36I	ACCTGC	1 cut(s)	576
AccI	GTMKAC	2 cut(s)	198, 312
AccII	CGCG	3 cut(s)	333, 1342, 1362
AciI	CCGC	6 cut(s)	27, 140, 325, 370, 1061, 1340
AclWI	GGATC	1 cut(s)	1141
AcoI	YGGCCR	2 cut(s)	322, 1435
AcsI	RAATTY	1 cut(s)	502
AcuI	CTGAAG	2 cut(s)	230, 1302
AcyI	GRCGYC	1 cut(s)	1164
AfaI	GTAC	3 cut(s)	926, 1154, 1241
AfiI	CCNNNNNNNGG	5 cut(s)	47, 213, 369, 396, 1294
AflIII	ACRYGT	1 cut(s)	1236
AgsI	TTSAA	5 cut(s)	253, 502, 1178, 1189, 1357
AhdI	GACNNNNNGTC	1 cut(s)	340
AluBI	AGCT	6 cut(s)	539, 659, 667, 746, 764, 869
AluI	AGCT	6 cut(s)	539, 659, 667, 746, 764, 869
AlwI	GGATC	1 cut(s)	1141
Ama87I	CYCGRG	2 cut(s)	389, 700
AoxI	GGCC	5 cut(s)	322, 360, 385, 392, 1435
ApeKI	GCWGC	1 cut(s)	431
ApoI	RAATTY	1 cut(s)	502
AspLEI	GCGC	2 cut(s)	333, 1081
AspS9I	GGNCC	6 cut(s)	230, 351, 361, 372, 386, 392
AsuC2I	CCSGG	3 cut(s)	48, 390, 391
AsuHPI	GGTGA	7 cut(s)	67, 75, 406, 1009, 1156, 1226, 1342
AvaI	CYCGRG	2 cut(s)	389, 700
AvaII	GGWCC	3 cut(s)	230, 351, 372
AxyI	CCTNAGG	1 cut(s)	492
BaeI	ACNNNNGTAYC	2 cut(s)	1144, 1177
BauI	CACGAG	1 cut(s)	356
BbsI	GAAGAC	3 cut(s)	177, 1290, 1433
BbvI	GCAGC	1 cut(s)	418
BccI	CCATC	6 cut(s)	273, 673, 883, 1198, 1312, 1364
BceAI	ACGGC	2 cut(s)	437, 1422
BcnI	CCSGG	3 cut(s)	48, 390, 391
BfoI	RGCGCY	1 cut(s)	1082
BfuAI	ACCTGC	1 cut(s)	576
BisI	GCNGC	4 cut(s)	27, 325, 432, 1340
BlpI	GCTNAGC	1 cut(s)	540
BlsI	GCNGC	4 cut(s)	28, 326, 433, 1341
Bme1390I	CCNGG	3 cut(s)	48, 390, 391
Bme18I	GGWCC	3 cut(s)	230, 351, 372
BmeRI	GACNNNNNGTC	1 cut(s)	340
BmeT110I	CYCGRG	2 cut(s)	389, 700
BmgT120I	GGNCC	6 cut(s)	230, 351, 361, 372, 386, 392
BmiI	GGNNCC	5 cut(s)	71, 232, 353, 374, 388
BmrFI	CCNGG	3 cut(s)	48, 390, 391
BpiI	GAAGAC	3 cut(s)	177, 1290, 1433
Bpu1102I	GCTNAGC	1 cut(s)	540
BpuEI	CTTGAG	1 cut(s)	84
BpuMI	CCSGG	3 cut(s)	48, 390, 391
BsaAI	YACGTR	1 cut(s)	1239
BsaHI	GRCGYC	1 cut(s)	1164
BsaJI	CCNNGG	3 cut(s)	388, 389, 395
BsaWI	WCCGGW	1 cut(s)	1149
BsaXI	ACNNNNNCTCC	2 cut(s)	677, 707
Bsc4I	CCNNNNNNNGG	5 cut(s)	47, 213, 369, 396, 1294
Bse118I	RCCGGY	1 cut(s)	320
Bse1I	ACTGG	1 cut(s)	185
Bse21I	CCTNAGG	1 cut(s)	492
BseDI	CCNNGG	3 cut(s)	388, 389, 395
BseGI	GGATG	4 cut(s)	91, 268, 1111, 1375
BseLI	CCNNNNNNNGG	5 cut(s)	47, 213, 369, 396, 1294
BseMII	CTCAG	1 cut(s)	506
BseNI	ACTGG	1 cut(s)	185
BseRI	GAGGAG	1 cut(s)	283
BseX3I	CGGCCG	1 cut(s)	322
BseXI	GCAGC	1 cut(s)	418
Bsh1236I	CGCG	3 cut(s)	333, 1342, 1362
Bsh1285I	CGRYCG	1 cut(s)	325
BshFI	GGCC	5 cut(s)	324, 362, 387, 394, 1437
BsiEI	CGRYCG	1 cut(s)	325
BsiHKCI	CYCGRG	2 cut(s)	389, 700
BsiSI	CCGG	5 cut(s)	48, 321, 390, 1150, 1167
BslFI	GGGAC	2 cut(s)	328, 358
BslI	CCNNNNNNNGG	5 cut(s)	47, 213, 369, 396, 1294
BsmFI	GGGAC	2 cut(s)	328, 358
BsmI	GAATGC	2 cut(s)	283, 372
BsnI	GGCC	5 cut(s)	324, 362, 387, 394, 1437
BsoBI	CYCGRG	2 cut(s)	389, 700
Bsp143I	GATC	2 cut(s)	598, 1146
Bsp1720I	GCTNAGC	1 cut(s)	540
BspACI	CCGC	6 cut(s)	27, 140, 325, 370, 1061, 1340
BspANI	GGCC	5 cut(s)	324, 362, 387, 394, 1437
BspCNI	CTCAG	1 cut(s)	505
BspFNI	CGCG	3 cut(s)	333, 1342, 1362
BspLI	GGNNCC	5 cut(s)	71, 232, 353, 374, 388
BspMI	ACCTGC	1 cut(s)	576
BspPI	GGATC	1 cut(s)	1141
BsrFI	RCCGGY	1 cut(s)	320
BsrI	ACTGG	1 cut(s)	185
BssAI	RCCGGY	1 cut(s)	320
BssECI	CCNNGG	3 cut(s)	388, 389, 395
BssMI	GATC	2 cut(s)	598, 1146
BssNI	GRCGYC	1 cut(s)	1164
BssSI	CACGAG	1 cut(s)	356
Bst2BI	CACGAG	1 cut(s)	356
Bst6I	CTCTTC	2 cut(s)	206, 785
BstACI	GRCGYC	1 cut(s)	1164
BstBAI	YACGTR	1 cut(s)	1239
BstC8I	GCNNGC	5 cut(s)	322, 329, 905, 1083, 1223
BstDEI	CTNAG	2 cut(s)	492, 540
BstF5I	GGATG	4 cut(s)	91, 268, 1111, 1375
BstFNI	CGCG	3 cut(s)	333, 1342, 1362
BstH2I	RGCGCY	1 cut(s)	1082
BstHHI	GCGC	2 cut(s)	333, 1081
BstKTI	GATC	2 cut(s)	601, 1149
BstMBI	GATC	2 cut(s)	598, 1146
BstMCI	CGRYCG	1 cut(s)	325
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 330, 1434
BstSCI	CCNGG	3 cut(s)	46, 388, 389
BstUI	CGCG	3 cut(s)	333, 1342, 1362
BstV1I	GCAGC	1 cut(s)	418
BstV2I	GAAGAC	3 cut(s)	177, 1290, 1433
BstZI	CGGCCG	1 cut(s)	322
Bsu36I	CCTNAGG	1 cut(s)	492
BsuRI	GGCC	5 cut(s)	324, 362, 387, 394, 1437
BtsCI	GGATG	4 cut(s)	91, 268, 1111, 1375
BveI	ACCTGC	1 cut(s)	576
Cac8I	GCNNGC	5 cut(s)	322, 329, 905, 1083, 1223
CfoI	GCGC	2 cut(s)	333, 1081
Cfr10I	RCCGGY	1 cut(s)	320
Cfr13I	GGNCC	6 cut(s)	230, 351, 361, 372, 386, 392
Cfr9I	CCCGGG	1 cut(s)	389
CseI	GACGC	1 cut(s)	1172
Csp6I	GTAC	3 cut(s)	925, 1153, 1240
CviAII	CATG	7 cut(s)	227, 476, 482, 836, 1058, 1069, 1261
CviQI	GTAC	3 cut(s)	925, 1153, 1240
DdeI	CTNAG	2 cut(s)	492, 540
DpnI	GATC	2 cut(s)	600, 1148
DpnII	GATC	2 cut(s)	598, 1146
DrdI	GACNNNNNNGTC	1 cut(s)	308
DriI	GACNNNNNGTC	1 cut(s)	340
DseDI	GACNNNNNNGTC	1 cut(s)	308
EaeI	YGGCCR	2 cut(s)	322, 1435
EagI	CGGCCG	1 cut(s)	322
Eam1104I	CTCTTC	2 cut(s)	206, 785
Eam1105I	GACNNNNNGTC	1 cut(s)	340
EarI	CTCTTC	2 cut(s)	206, 785
EclXI	CGGCCG	1 cut(s)	322
Eco47I	GGWCC	3 cut(s)	230, 351, 372
Eco52I	CGGCCG	1 cut(s)	322
Eco57I	CTGAAG	2 cut(s)	230, 1302
Eco81I	CCTNAGG	1 cut(s)	492
Eco88I	CYCGRG	2 cut(s)	389, 700
EcoRI	GAATTC	1 cut(s)	502
EcoT22I	ATGCAT	1 cut(s)	477
FaeI	CATG	7 cut(s)	230, 479, 485, 839, 1061, 1072, 1264
FalI	AAGNNNNNCTT	2 cut(s)	891, 923
FaqI	GGGAC	2 cut(s)	328, 358
FatI	CATG	7 cut(s)	226, 475, 481, 835, 1057, 1068, 1260
FauI	CCCGC	2 cut(s)	147, 1054
FblI	GTMKAC	2 cut(s)	198, 312
Fnu4HI	GCNGC	4 cut(s)	27, 325, 432, 1340
FokI	GGATG	4 cut(s)	78, 255, 1118, 1382
Fsp4HI	GCNGC	4 cut(s)	27, 325, 432, 1340
GlaI	GCGC	2 cut(s)	332, 1080
GluI	GCNGC	4 cut(s)	27, 325, 432, 1340
HaeII	RGCGCY	1 cut(s)	1082
HaeIII	GGCC	5 cut(s)	324, 362, 387, 394, 1437
HapII	CCGG	5 cut(s)	48, 321, 390, 1150, 1167
HgaI	GACGC	1 cut(s)	1172
HhaI	GCGC	2 cut(s)	333, 1081
Hin1I	GRCGYC	1 cut(s)	1164
Hin1II	CATG	7 cut(s)	230, 479, 485, 839, 1061, 1072, 1264
Hin6I	GCGC	2 cut(s)	331, 1079
HinP1I	GCGC	2 cut(s)	331, 1079
HincII	GTYRAC	2 cut(s)	199, 313
HindII	GTYRAC	2 cut(s)	199, 313
HindIII	AAGCTT	2 cut(s)	657, 762
HinfI	GANTC	5 cut(s)	104, 124, 157, 223, 937
HpaII	CCGG	5 cut(s)	48, 321, 390, 1150, 1167
HphI	GGTGA	7 cut(s)	67, 75, 406, 1009, 1156, 1226, 1342
Hpy166II	GTNNAC	3 cut(s)	199, 313, 1350
Hpy188I	TCNGA	7 cut(s)	69, 136, 190, 273, 743, 753, 1131
Hpy188III	TCNNGA	7 cut(s)	101, 220, 349, 494, 521, 1232, 1367
Hpy8I	GTNNAC	3 cut(s)	199, 313, 1350
Hpy99I	CGWCG	2 cut(s)	317, 715
HpyAV	CCTTC	7 cut(s)	96, 307, 893, 921, 1096, 1338, 1363
HpyCH4IV	ACGT	4 cut(s)	315, 713, 923, 1238
HpyCH4V	TGCA	5 cut(s)	13, 475, 781, 1403, 1423
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 330, 1434
HpyF3I	CTNAG	2 cut(s)	492, 540
HpySE526I	ACGT	4 cut(s)	315, 713, 923, 1238
Hsp92I	GRCGYC	1 cut(s)	1164
Hsp92II	CATG	7 cut(s)	230, 479, 485, 839, 1061, 1072, 1264
HspAI	GCGC	2 cut(s)	331, 1079
KroI	GCCGGC	1 cut(s)	320
KroNI	GCCGGC	1 cut(s)	322
Kzo9I	GATC	2 cut(s)	598, 1146
LmnI	GCTCC	3 cut(s)	69, 296, 664
Lsp1109I	GCAGC	1 cut(s)	418
MaeII	ACGT	4 cut(s)	315, 713, 923, 1238
MaeIII	GTNAC	5 cut(s)	608, 814, 1021, 1160, 1381
MalI	GATC	2 cut(s)	600, 1148
MboI	GATC	2 cut(s)	598, 1146
MluCI	AATT	7 cut(s)	57, 245, 502, 516, 562, 618, 1457
MlyI	GAGTC	1 cut(s)	98
MmeI	TCCRAC	3 cut(s)	47, 159, 1071
Mph1103I	ATGCAT	1 cut(s)	477
MroNI	GCCGGC	1 cut(s)	320
MslI	CAYNNNNRTG	2 cut(s)	480, 1265
MspI	CCGG	5 cut(s)	48, 321, 390, 1150, 1167
MspR9I	CCNGG	3 cut(s)	48, 390, 391
Mva1269I	GAATGC	2 cut(s)	283, 372
MvnI	CGCG	3 cut(s)	333, 1342, 1362
MwoI	GCNNNNNNNGC	3 cut(s)	10, 330, 1434
NaeI	GCCGGC	1 cut(s)	322
NciI	CCSGG	3 cut(s)	48, 390, 391
NdeII	GATC	2 cut(s)	598, 1146
NgoMIV	GCCGGC	1 cut(s)	320
NlaIII	CATG	7 cut(s)	230, 479, 485, 839, 1061, 1072, 1264
NlaIV	GGNNCC	5 cut(s)	71, 232, 353, 374, 388
NmeAIII	GCCGAG	2 cut(s)	363, 420
NmuCI	GTSAC	2 cut(s)	1021, 1160
NsiI	ATGCAT	1 cut(s)	477
PcsI	WCGNNNNNNNCGW	2 cut(s)	312, 920
PctI	GAATGC	2 cut(s)	283, 372
PdiI	GCCGGC	1 cut(s)	322
PfeI	GAWTC	4 cut(s)	124, 157, 223, 937
PkrI	GCNGC	4 cut(s)	28, 326, 433, 1341
PleI	GAGTC	1 cut(s)	98
PpsI	GAGTC	1 cut(s)	98
Ppu21I	YACGTR	1 cut(s)	1239
PsiI	TTATAA	1 cut(s)	1032
PspN4I	GGNNCC	5 cut(s)	71, 232, 353, 374, 388
PspPI	GGNCC	6 cut(s)	230, 351, 361, 372, 386, 392
RsaI	GTAC	3 cut(s)	926, 1154, 1241
RsaNI	GTAC	3 cut(s)	925, 1153, 1240
RseI	CAYNNNNRTG	2 cut(s)	480, 1265
SalI	GTCGAC	2 cut(s)	197, 311
SatI	GCNGC	4 cut(s)	27, 325, 432, 1340
Sau3AI	GATC	2 cut(s)	598, 1146
Sau96I	GGNCC	6 cut(s)	230, 351, 361, 372, 386, 392
SchI	GAGTC	1 cut(s)	98
ScrFI	CCNGG	3 cut(s)	48, 390, 391
SinI	GGWCC	3 cut(s)	230, 351, 372
SmaI	CCCGGG	1 cut(s)	391
SmiMI	CAYNNNNRTG	2 cut(s)	480, 1265
SmlI	CTYRAG	1 cut(s)	99
SmoI	CTYRAG	1 cut(s)	99
Sse9I	AATT	7 cut(s)	57, 245, 502, 516, 562, 618, 1457
SsiI	CCGC	6 cut(s)	27, 140, 325, 370, 1061, 1340
StyD4I	CCNGG	3 cut(s)	46, 388, 389
TaiI	ACGT	4 cut(s)	318, 716, 926, 1241
TaqI	TCGA	7 cut(s)	177, 198, 261, 300, 312, 871, 882
TasI	AATT	7 cut(s)	57, 245, 502, 516, 562, 618, 1457
TauI	GCSGC	3 cut(s)	29, 327, 1342
TfiI	GAWTC	4 cut(s)	124, 157, 223, 937
TseFI	GTSAC	2 cut(s)	1021, 1160
TseI	GCWGC	1 cut(s)	431
Tsp45I	GTSAC	2 cut(s)	1021, 1160
TspDTI	ATGAA	4 cut(s)	492, 800, 824, 1115
TspGWI	ACGGA	1 cut(s)	51
TspMI	CCCGGG	1 cut(s)	389
VpaK11BI	GGWCC	3 cut(s)	230, 351, 372
XapI	RAATTY	1 cut(s)	502
XmaI	CCCGGG	1 cut(s)	389
XmiI	GTMKAC	2 cut(s)	198, 312
Zsp2I	ATGCAT	1 cut(s)	477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.