AT1G75530

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005427
Physical Location & Seq
Forward (+)
28359309 .. 28361815
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75530.1

Sequence Viewer

Length: 1668 bp
ATGGCGTTTCAGTGGTTACCTGAAGATGATTATCTTCTCAGAAAATCACTCGAGGATGGAACTTCTCTAGAGACGTTAGCTAAAGGAGCGGTGAGATTCTCTCGAAAATTTACACTTTCAGAACTAACAGAGCGATGGCATTGTCTTCTTTACAATCCCAAGGTTACATCTCTGTCTTCATCAGTCGGATTTGAGCTCCAATACGGAGCTCAATTCGTTCCACAAAGTCTCTTTGATTCAGTACCAGTCAGAACTCATTACTACACTACACGAAAAAGGCGGCGTTTGGAGTTAGAGGAGTCTCTTAAGGTCAATAACAATGCCATTGATGAACATCTTAATGTGGAGGAAGGGAATTTGTTTGGAGAATGTGATGATGATTTTGGTTTCAAGTTTGAGGACATTGAAAGTTTTCGCAACACTTTCCCAGACATTTTGCTTTCCCCTCATGATTATCAGACAACTATCTTTGGTAATGAGATTGATGATGAGGATGATCGAATGGTGAATCAGATGTTAAACATCAACGATGACCAGATTCATGACTCTTATGTTACAGACACAATAACAACAACAACAGAACATGTTCTGCTTCAAGAAGTGTTTCAAGATCCTCTGTTTCAACAACCTAACGCATCCTTGAATGAGCCAGCATCACCGTCACAGCAGCAAGTAATCCTTGATCAAGCAGAGATTTGGATTCCTCCACCGCCTTATGATCCACATCCAGAAATCGTTAACGGTGTTATCAATTGTGTTATAAACCGTGAGTCCGATGAGATTCCAGATAATGATGATATCAACCTGCAACTGTACAATCCTAAAGCTCGAAACTCGGTTAATCCTTCATCAGTGAGGAATAATATGAAGCCACCTCTGCCTCCAGTTAGAAGATCATCCTTACAAGTCAAGAGCAAAGACATTATCGACAGTTCTGGTTTTGGAGATTGTGCTGTCACAACCCGAGCAAGTTCTTCTTCTGCATTCAGTGATAGCTTTACTGAGAAAGCGACATCTACTGTTGCTGCAACAACTTCATCAACTCTACAACACTTTCCAGAGAATGAAATTTGTGAACAGACATTGAGTGCAGAGATGGATATAAACGTACCAGAGGAGAATAATATTGAAATTGAGAGTGATGAAGAGTTGCCTTCCTTTTCAGATCTAGAAGCTATGATTCTTGACATGGACCTTGAACCGATTGGTCAAGACCAGTACGAGTTGGACGCTTCAAAATATCGAAACGAGGAAATGGCGCGAAAGATAATGAGATTAGAGCAGAGTGCTGAATCTTACATGAACCGTGACATCGCTGCTCATGGAGCTTTCGCTCTTCTATATGGAAGCTCTAAGCATTACATCAATAAACCAGAGGTGTTACTTGGAAGAGCAACAGGTGAATATCCGGTGGATATCGACTTAGGAAGGTCAGGGTCTGAAACAAGATTCTCTCGACGACAGGCTCTAATAAAGTTGAAGCAAGATGGGAGTTTTGAAATCAAGAATCTTGGGAAGTTTTCGATATGGATGAACGATGAGGAAATTAATCATGGAGAAGTTGTCATTCTCAAGAACAATTGCTTAATCCAGATACGAGAAAAGTCGTTCATATTCGAGAAGAATGAGAAAGCAGTCAAAAGATATTTAGATGGAATTCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

555

Amino Acids

63.27

Weight (kDa)

4.63

Isoelectric Point (pI)

60.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 4 - 60 1.3e-12 N-terminal region of micro-spherule protein
FHA PF00498 461 - 532 8.3e-08 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 813
AccBSI CCGCTC 1 cut(s) 89
AccII CGCG 1 cut(s) 1261
AciI CCGC 3 cut(s) 89, 280, 710
AclWI GGATC 2 cut(s) 605, 713
AcsI RAATTY 4 cut(s) 107, 355, 1068, 1656
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 4 cut(s) 243, 815, 1110, 1220
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 583
AluBI AGCT 8 cut(s) 80, 196, 209, 827, 996, 1175, 1328, 1350
AluI AGCT 8 cut(s) 80, 196, 209, 827, 996, 1175, 1328, 1350
Alw21I GWGCWC 2 cut(s) 198, 211
Alw26I GTCTC 3 cut(s) 65, 233, 306
AlwI GGATC 2 cut(s) 605, 713
AlwNI CAGNNNCTG 1 cut(s) 1439
Ama87I CYCGRG 2 cut(s) 50, 963
ApeKI GCWGC 3 cut(s) 667, 1025, 1316
ApoI RAATTY 4 cut(s) 107, 355, 1068, 1656
AseI ATTAAT 1 cut(s) 1548
Asp700I GAANNNNTTC 3 cut(s) 411, 585, 603
AspLEI GCGC 1 cut(s) 1261
AspS9I GGNCC 1 cut(s) 1192
AsuHPI GGTGA 4 cut(s) 103, 517, 648, 1412
AvaI CYCGRG 2 cut(s) 50, 963
AvaII GGWCC 1 cut(s) 1192
BanII GRGCYC 2 cut(s) 198, 211
BbsI GAAGAC 2 cut(s) 137, 168
Bbv12I GWGCWC 2 cut(s) 198, 211
BbvI GCAGC 3 cut(s) 679, 1012, 1303
BccI CCATC 5 cut(s) 50, 129, 1090, 1481, 1646
BclI TGATCA 1 cut(s) 682
BcoDI GTCTC 3 cut(s) 65, 233, 306
BfaI CTAG 2 cut(s) 68, 1169
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 813
BglII AGATCT 1 cut(s) 1165
BisI GCNGC 4 cut(s) 281, 668, 1026, 1317
BlsI GCNGC 4 cut(s) 282, 669, 1027, 1318
Bme18I GGWCC 1 cut(s) 1192
BmeT110I CYCGRG 2 cut(s) 50, 963
BmgT120I GGNCC 1 cut(s) 1192
BmsI GCATC 2 cut(s) 644, 662
BpiI GAAGAC 2 cut(s) 137, 168
BplI GAGNNNNNCTC 2 cut(s) 85, 117
BpmI CTGGAG 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 1556
BsaBI GATNNNNATC 3 cut(s) 30, 333, 723
BsaJI CCNNGG 1 cut(s) 159
BsaWI WCCGGW 1 cut(s) 1408
BsaXI ACNNNNNCTCC 2 cut(s) 1548, 1578
Bse1I ACTGG 3 cut(s) 245, 884, 1216
Bse8I GATNNNNATC 3 cut(s) 30, 333, 723
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 6 cut(s) 61, 499, 635, 724, 896, 1536
BseJI GATNNNNATC 3 cut(s) 30, 333, 723
BseMII CTCAG 2 cut(s) 52, 993
BseNI ACTGG 3 cut(s) 245, 884, 1216
BseRI GAGGAG 2 cut(s) 311, 1130
BseXI GCAGC 3 cut(s) 679, 1012, 1303
BsgI GTGCAG 1 cut(s) 1110
Bsh1236I CGCG 1 cut(s) 1261
BsiHKAI GWGCWC 2 cut(s) 198, 211
BsiHKCI CYCGRG 2 cut(s) 50, 963
BsiSI CCGG 1 cut(s) 1409
BsmAI GTCTC 3 cut(s) 65, 233, 306
BsmBI CGTCTC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 983
BsoBI CYCGRG 2 cut(s) 50, 963
Bsp1286I GDGCHC 2 cut(s) 198, 211
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
BspACI CCGC 3 cut(s) 89, 280, 710
BspCNI CTCAG 2 cut(s) 51, 994
BspFNI CGCG 1 cut(s) 1261
BspHI TCATGA 2 cut(s) 448, 541
BspMI ACCTGC 1 cut(s) 813
BspPI GGATC 2 cut(s) 605, 713
BspQI GCTCTTC 2 cut(s) 1341, 1384
BspTI CTTAAG 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 3 cut(s) 245, 884, 1216
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
BssT1I CCWWGG 1 cut(s) 159
Bst4CI ACNGT 7 cut(s) 660, 743, 767, 813, 932, 1021, 1307
Bst6I CTCTTC 3 cut(s) 1140, 1341, 1384
BstAFI CTTAAG 1 cut(s) 305
BstAUI TGTACA 1 cut(s) 813
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 4 cut(s) 38, 1002, 1353, 1423
BstEII GGTNACC 1 cut(s) 15
BstF5I GGATG 6 cut(s) 61, 499, 635, 724, 896, 1536
BstFNI CGCG 1 cut(s) 1261
BstHHI GCGC 1 cut(s) 1261
BstKTI GATC 6 cut(s) 499, 613, 685, 721, 896, 1168
BstMAI GTCTC 3 cut(s) 65, 233, 306
BstMBI GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
BstMWI GCNNNNNNNGC 3 cut(s) 86, 877, 1325
BstNSI RCATGY 1 cut(s) 587
BstPI GGTNACC 1 cut(s) 15
BstUI CGCG 1 cut(s) 1261
BstV1I GCAGC 3 cut(s) 679, 1012, 1303
BstV2I GAAGAC 2 cut(s) 137, 168
BstX2I RGATCY 2 cut(s) 610, 1165
BstYI RGATCY 2 cut(s) 610, 1165
BtgZI GCGATG 2 cut(s) 148, 1297
BtsCI GGATG 6 cut(s) 61, 499, 635, 724, 896, 1536
BtsIMutI CAGTG 3 cut(s) 17, 858, 994
BveI ACCTGC 1 cut(s) 813
Cac8I GCNNGC 1 cut(s) 651
CaiI CAGNNNCTG 1 cut(s) 1439
CciI TCATGA 2 cut(s) 448, 541
CfoI GCGC 1 cut(s) 1261
Cfr13I GGNCC 1 cut(s) 1192
CseI GACGC 1 cut(s) 1238
Csp6I GTAC 4 cut(s) 242, 814, 1109, 1219
CviAII CATG 7 cut(s) 449, 542, 584, 1189, 1300, 1322, 1553
CviQI GTAC 4 cut(s) 242, 814, 1109, 1219
DdeI CTNAG 4 cut(s) 38, 1002, 1353, 1423
DpnI GATC 6 cut(s) 498, 612, 684, 720, 895, 1167
DpnII GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
Eam1104I CTCTTC 3 cut(s) 1140, 1341, 1384
EarI CTCTTC 3 cut(s) 1140, 1341, 1384
Ecl136II GAGCTC 2 cut(s) 196, 209
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 2 cut(s) 198, 211
Eco32I GATATC 2 cut(s) 799, 1417
Eco47I GGWCC 1 cut(s) 1192
Eco53kI GAGCTC 2 cut(s) 196, 209
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 2 cut(s) 50, 963
Eco91I GGTNACC 1 cut(s) 15
EcoICRI GAGCTC 2 cut(s) 196, 209
EcoO65I GGTNACC 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 1656
EcoRV GATATC 2 cut(s) 799, 1417
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 2 cut(s) 198, 211
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 7 cut(s) 452, 545, 587, 1192, 1303, 1325, 1556
FalI AAGNNNNNCTT 4 cut(s) 663, 695, 961, 993
FatI CATG 7 cut(s) 448, 541, 583, 1188, 1299, 1321, 1552
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 4 cut(s) 281, 668, 1026, 1317
FokI GGATG 6 cut(s) 68, 506, 622, 711, 883, 1543
FriOI GRGCYC 2 cut(s) 198, 211
Fsp4HI GCNGC 4 cut(s) 281, 668, 1026, 1317
FspBI CTAG 2 cut(s) 68, 1169
GlaI GCGC 1 cut(s) 1260
GluI GCNGC 4 cut(s) 281, 668, 1026, 1317
GsuI CTGGAG 1 cut(s) 867
HapII CCGG 1 cut(s) 1409
HgaI GACGC 1 cut(s) 1238
HhaI GCGC 1 cut(s) 1261
Hin1II CATG 7 cut(s) 452, 545, 587, 1192, 1303, 1325, 1556
Hin6I GCGC 1 cut(s) 1259
HinP1I GCGC 1 cut(s) 1259
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 1 cut(s) 1409
HphI GGTGA 4 cut(s) 103, 517, 648, 1412
Hpy166II GTNNAC 2 cut(s) 739, 1076
Hpy188I TCNGA 9 cut(s) 41, 121, 188, 251, 459, 513, 775, 1165, 1441
Hpy8I GTNNAC 2 cut(s) 739, 1076
Hpy99I CGWCG 1 cut(s) 1461
HpyAV CCTTC 4 cut(s) 344, 855, 1164, 1422
HpyCH4III ACNGT 7 cut(s) 660, 743, 767, 813, 932, 1021, 1307
HpyCH4IV ACGT 2 cut(s) 74, 1107
HpyCH4V TGCA 4 cut(s) 808, 983, 1028, 1091
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 877, 1325
HpyF3I CTNAG 4 cut(s) 38, 1002, 1353, 1423
HpySE526I ACGT 2 cut(s) 74, 1107
Hsp92II CATG 7 cut(s) 452, 545, 587, 1192, 1303, 1325, 1556
HspAI GCGC 1 cut(s) 1259
Ksp22I TGATCA 1 cut(s) 682
KspAI GTTAAC 1 cut(s) 739
Kzo9I GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
LguI GCTCTTC 2 cut(s) 1341, 1384
LmnI GCTCC 4 cut(s) 86, 201, 206, 1325
Lsp1109I GCAGC 3 cut(s) 679, 1012, 1303
LweI GCATC 2 cut(s) 644, 662
MaeI CTAG 2 cut(s) 68, 1169
MaeII ACGT 2 cut(s) 74, 1107
MaeIII GTNAC 7 cut(s) 15, 163, 553, 660, 955, 1307, 1380
MalI GATC 6 cut(s) 498, 612, 684, 720, 895, 1167
MbiI CCGCTC 1 cut(s) 89
MboI GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
MfeI CAATTG 2 cut(s) 751, 1579
MflI RGATCY 2 cut(s) 610, 1165
MhlI GDGCHC 2 cut(s) 198, 211
MluCI AATT 9 cut(s) 107, 212, 355, 751, 1068, 1131, 1545, 1579, 1656
MlyI GAGTC 3 cut(s) 308, 539, 779
MmeI TCCRAC 2 cut(s) 166, 1206
MroXI GAANNNNTTC 3 cut(s) 411, 585, 603
MseI TTAA 7 cut(s) 306, 339, 518, 738, 840, 1548, 1586
MslI CAYNNNNRTG 1 cut(s) 339
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 1 cut(s) 1409
MunI CAATTG 2 cut(s) 751, 1579
Mva1269I GAATGC 1 cut(s) 983
MvnI CGCG 1 cut(s) 1261
MwoI GCNNNNNNNGC 3 cut(s) 86, 877, 1325
NdeII GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
NlaIII CATG 7 cut(s) 452, 545, 587, 1192, 1303, 1325, 1556
NmuCI GTSAC 3 cut(s) 660, 955, 1307
NspI RCATGY 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 2 cut(s) 448, 541
PciI ACATGT 1 cut(s) 583
PciSI GCTCTTC 2 cut(s) 1341, 1384
PctI GAATGC 1 cut(s) 983
PdmI GAANNNNTTC 3 cut(s) 411, 585, 603
PkrI GCNGC 4 cut(s) 282, 669, 1027, 1318
PleI GAGTC 3 cut(s) 307, 539, 778
PpsI GAGTC 3 cut(s) 307, 539, 778
PscI ACATGT 1 cut(s) 583
PshBI ATTAAT 1 cut(s) 1548
PsiI TTATAA 1 cut(s) 761
Psp124BI GAGCTC 2 cut(s) 198, 211
PspEI GGTNACC 1 cut(s) 15
PspPI GGNCC 1 cut(s) 1192
PspXI VCTCGAGB 1 cut(s) 50
PstNI CAGNNNCTG 1 cut(s) 1439
PsuI RGATCY 2 cut(s) 610, 1165
RsaI GTAC 4 cut(s) 243, 815, 1110, 1220
RsaNI GTAC 4 cut(s) 242, 814, 1109, 1219
RseI CAYNNNNRTG 1 cut(s) 339
SacI GAGCTC 2 cut(s) 198, 211
SapI GCTCTTC 2 cut(s) 1341, 1384
SaqAI TTAA 7 cut(s) 306, 339, 518, 738, 840, 1548, 1586
SatI GCNGC 4 cut(s) 281, 668, 1026, 1317
Sau3AI GATC 6 cut(s) 496, 610, 682, 718, 893, 1165
Sau96I GGNCC 1 cut(s) 1192
SchI GAGTC 3 cut(s) 308, 539, 779
SduI GDGCHC 2 cut(s) 198, 211
SfaNI GCATC 2 cut(s) 644, 662
Sfr274I CTCGAG 1 cut(s) 50
SinI GGWCC 1 cut(s) 1192
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 3 cut(s) 50, 305, 1571
SmoI CTYRAG 3 cut(s) 50, 305, 1571
Sse9I AATT 9 cut(s) 107, 212, 355, 751, 1068, 1131, 1545, 1579, 1656
SsiI CCGC 3 cut(s) 89, 280, 710
SspI AATATT 1 cut(s) 1126
SspMI CTAG 2 cut(s) 68, 1169
SstI GAGCTC 2 cut(s) 198, 211
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 7 cut(s) 660, 743, 767, 813, 932, 1021, 1307
TaiI ACGT 2 cut(s) 77, 1110
TasI AATT 9 cut(s) 107, 212, 355, 751, 1068, 1131, 1545, 1579, 1656
TatI WGTACW 1 cut(s) 813
TauI GCSGC 1 cut(s) 283
Tru1I TTAA 7 cut(s) 306, 339, 518, 738, 840, 1548, 1586
Tru9I TTAA 7 cut(s) 306, 339, 518, 738, 840, 1548, 1586
TscAI CASTG 3 cut(s) 17, 858, 994
TseFI GTSAC 3 cut(s) 660, 955, 1307
TseI GCWGC 3 cut(s) 667, 1025, 1316
Tsp45I GTSAC 3 cut(s) 660, 955, 1307
TspGWI ACGGA 1 cut(s) 219
TspRI CASTG 3 cut(s) 17, 858, 994
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 1192
VspI ATTAAT 1 cut(s) 1548
XapI RAATTY 4 cut(s) 107, 355, 1068, 1656
XbaI TCTAGA 2 cut(s) 67, 1168
XceI RCATGY 1 cut(s) 587
XhoI CTCGAG 1 cut(s) 50
XmnI GAANNNNTTC 3 cut(s) 411, 585, 603
XspI CTAG 2 cut(s) 68, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.