MD15G1019100.v1.1

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005427
Physical Location & Seq
Reverse (-)
1098823 .. 1104094
5272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1019100.v1.1.491

Sequence Viewer

Length: 2517 bp
ATGGGTGCTCTCGCTCCGTTCTCTCAGTGGATTCCCGCGGATGATATTCTGCTCAAGAAAGCCGTTGAGGATGGTGCTTCCTTGGAGTCACTTGCTAAAGGTGCAGTGCATTTTTCTCGAAGATTTACTGTTTGTGAACTGCAAGATCGGTGGTATTCTCTCCTGTATGATCCAGTAGTTTCTGAAGATGCTTCTTCTCGCATGATTGAGTTTGAGAGTTCCACAACAACTGTTCCATTTGACAGGGCAGGAAATTCAAAAGGAAAAAAATGTGAATCCGGGAAGAGAAAAGCTGAAAGTGTTCGTAGTAGTTACTATGCAATGCGTAAAAGGATTTGCAACGAGCCGTTTAATTCCATGGGCCTTAATTTTCTTGTTGCACCCAGTAATAACAACTATGATGGAAATGGAGATGAGCCTCTTTACGGAAATTGCATGAATGGAGATCCAATCTCAGCCCCTTTTGGCCTCGAGACATCAGATATGGATGCAATGCAAAATCTGATGGATGTCGGCACTGATGATACTTTTCATATGCTCCAGAACCCAGATGGGAATGATTTTCATATGGAACAAGGTAATATCCATGAAGATATTATGACATGGAATGAATCTGAGGTGGATGAATTCAATCATCCAGAGGGATTGCCAGATTGCAGCCTGTTCAATGCCAATGACTTAGGAATGAAACCTCCGTTTACACTTGATCAAATTACTGGCAACGAGGGAAACATGTGCACAGAGTTTGAAGGGAACAAGGCCTTTAATTCACCTGTTTCAGACAGTGGTGCATTATTTAACAACTTGGAGTATTCATCTCCACTTCCAGCTATGCCAGTTGATGTTGATCTTAGAGAAAATGATATACGCACCAGTGATTCTTTTGAACTTCATGAAAATATCGAGGCTAACAAGGCAAGAACAACAGAGAATGATGCTCATATTGGGATGGAGGTGGCCACTGAAATGCCATGCTGTGATTTCCAGAGTTCTGCAGCTCCTGTTTGTACTGAAGGTTATTTGGCAGAATTGTCCAATTCACTTTTAAACTTTACGAATGAGGAGCTCATGCTTGTGACTGCTGATGGAAAAGATGTGATTAATAAGTCTTACTATGATGGCCTGAGCTCCCTTTTGTTGAGTTCACCGAAAGATGATGTTCTTCATGAACAGACAATCGTCAAAACTGAGCCAGAAACATCTGTAGCTCCAGTTATGGACTCCATGAACCCATCTAGTGCGTATCCTGGAGTGGTAGATGACAATAGAGGATCCCCAAATGCTGATGAACATATAGTCTGCCATTCAGACACTCCGATGCTATCATCTTCAACAGCTTCGAACTATCAAAATCCTGAATTGAGGGATGGATTTATCTGCTGCACATTAAACACCGAGGACCCAGAAATCCCATGCAATGGTGATATTTTTCTACCAAATCGTTCATCAACTTCTGAAGGGAATAAGCCAATATCTTTGTCCACTAGTGGTTTCCCTGTTAATAAGAGAAATAATGACACAGGGCCGTGCTTTATGCATAAAGAGCGAAAAAATCTTGGAAAACCACATAGTACCTGTCAGATTAAAGGATCACATTTCTTACAAGAAAAGGGTCGAAAATCTCCACTAGGTAATTTCGGGGTGAAATTTGAGCTGTCTGATGTCCCCAGTGAGGTGGCATCTAAGAGTGCAGGTCATGTTGGTGAAGGTCCGGGCCAAATTGATTCAGCCAATCCAAGTACAAGTGTTCAACCTGGAATACTGAAGGAAGACACTAAAGAAAGTGTATCGGAAAAGCGTCTCAGTTATAACTCAACTGAGTTTCATGTGGAGAACCCAGATTCTGGTGTCATTAAAAAGGAGCTTGATCCATCAGCTACAATTAGAGACGATATATCATTACATACAGAAGGGCTGCCCATGTATATTGCTGAACCACAATTAAATCCCGATACATCGGATCAACTGGGGCTTTTTGAGAGCGATGATGATATACCATGTTATTCGGATATAGAGGCAATGGTACTTGATATGGATTTGGACCCAGATGACCAGGATTTGTGTTCTAGTGAGGAAGTCTCAAAATATCAAAATGAGGATGCTAAGAGGAGAATCATAAGGCTGGAGCAGTGTGCTTATTCATATTTGCAAAGAGCCATTGCATCTCATGGAGCTTTTGCCATTTTGTATGGACGTCATTCTAAGCATTACATTAAGAAGCCCGAGGTTCTATTGGGTAGAGCAACAGACGATGCTGTTGTTGACATTGACTTGGGAAGAGACGGGCGTGGTAATAAGATCTCTCGGAAACAGGCAATGATAAATATGGATAAAGATGGATCCTTCCATCTGAAAAATCTTGGGAAGTGTTCAATCTCGGTAAATAGCAAGGAAGTAGCCCCTGGACAGAGTCTAAGCCTCAGTTCAAGTTGCTTGATTGAGATACGGGGAATGCCATTCATATTTGAGATGAACCAAACACGCGTGAAGCAGTATTTGGAAAGCATCCCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

839

Amino Acids

92.33

Weight (kDa)

4.62

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 9 - 75 3.5e-14 N-terminal region of micro-spherule protein
FHA PF00498 744 - 815 9.9e-06 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1809
AatII GACGTC 1 cut(s) 2197
Acc36I ACCTGC 1 cut(s) 1682
AccB7I CCANNNNNTGG 2 cut(s) 1418, 1844
AccII CGCG 2 cut(s) 38, 2484
AciI CCGC 2 cut(s) 36, 38
AclWI GGATC 9 cut(s) 164, 440, 1266, 1279, 1597, 1862, 1968, 2334, 2347
AcoI YGGCCR 1 cut(s) 957
AcsI RAATTY 3 cut(s) 253, 626, 1646
AcuI CTGAAG 4 cut(s) 204, 1032, 1476, 1784
AcyI GRCGYC 1 cut(s) 2194
AfaI GTAC 4 cut(s) 1009, 1573, 1741, 2025
AfiI CCNNNNNNNGG 4 cut(s) 425, 1418, 1672, 1844
AflIII ACRYGT 2 cut(s) 732, 2482
AgsI TTSAA 9 cut(s) 258, 631, 667, 749, 887, 1332, 1751, 2373, 2427
AhlI ACTAGT 1 cut(s) 1484
AjnI CCWGG 4 cut(s) 1246, 1753, 2052, 2401
AjuI GAANNNNNNNTTGG 2 cut(s) 2480, 2512
Alw21I GWGCWC 4 cut(s) 10, 740, 1068, 1130
Alw26I GTCTC 5 cut(s) 467, 1805, 1881, 2083, 2274
Alw44I GTGCAC 1 cut(s) 736
AlwI GGATC 9 cut(s) 164, 440, 1266, 1279, 1597, 1862, 1968, 2334, 2347
AlwNI CAGNNNCTG 2 cut(s) 1001, 1844
Ama87I CYCGRG 2 cut(s) 470, 2222
AoxI GGCC 7 cut(s) 361, 466, 759, 957, 1120, 1523, 1714
ApaLI GTGCAC 1 cut(s) 736
ApeKI GCWGC 4 cut(s) 657, 995, 1380, 1915
ApoI RAATTY 3 cut(s) 253, 626, 1646
AseI ATTAAT 1 cut(s) 1101
Asp700I GAANNNNTTC 1 cut(s) 300
AspS9I GGNCC 6 cut(s) 361, 1399, 1523, 1709, 1714, 2041
AsuC2I CCSGG 2 cut(s) 280, 1713
AsuHPI GGTGA 5 cut(s) 762, 1137, 1433, 1654, 1715
AsuII TTCGAA 1 cut(s) 1340
AvaI CYCGRG 2 cut(s) 470, 2222
AvaII GGWCC 3 cut(s) 1399, 1709, 2041
BaeGI GKGCMC 1 cut(s) 740
BalI TGGCCA 1 cut(s) 959
BamHI GGATCC 2 cut(s) 1271, 2339
BanII GRGCYC 2 cut(s) 1068, 1130
BbsI GAAGAC 1 cut(s) 1776
Bbv12I GWGCWC 4 cut(s) 10, 740, 1068, 1130
BbvI GCAGC 4 cut(s) 669, 1007, 1367, 1902
BceAI ACGGC 3 cut(s) 47, 331, 1510
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 4 cut(s) 1248, 1755, 2054, 2403
BciVI GTATCC 1 cut(s) 1254
BclI TGATCA 1 cut(s) 706
BcnI CCSGG 2 cut(s) 280, 1713
BcoDI GTCTC 5 cut(s) 467, 1805, 1881, 2083, 2274
BcuI ACTAGT 1 cut(s) 1484
BfaI CTAG 4 cut(s) 1236, 1485, 1628, 2067
BfmI CTRYAG 2 cut(s) 993, 1203
BfuAI ACCTGC 1 cut(s) 1682
BfuI GTATCC 1 cut(s) 1254
BglII AGATCT 1 cut(s) 2298
BisI GCNGC 4 cut(s) 658, 996, 1381, 1916
BlsI GCNGC 4 cut(s) 659, 997, 1382, 1917
Bme1390I CCNGG 6 cut(s) 280, 1248, 1713, 1755, 2054, 2403
Bme18I GGWCC 3 cut(s) 1399, 1709, 2041
BmeT110I CYCGRG 2 cut(s) 470, 2222
BmgT120I GGNCC 6 cut(s) 361, 1399, 1523, 1709, 1714, 2041
BmiI GGNNCC 4 cut(s) 1273, 1401, 2043, 2341
BmrFI CCNGG 6 cut(s) 280, 1248, 1713, 1755, 2054, 2403
BmrI ACTGGG 3 cut(s) 378, 1662, 1976
BmsI GCATC 8 cut(s) 178, 478, 925, 1308, 1688, 2089, 2171, 2242
BmuI ACTGGG 3 cut(s) 378, 1662, 1976
BoxI GACNNNNGTC 1 cut(s) 1178
BpiI GAAGAC 1 cut(s) 1776
BplI GAGNNNNNCTC 2 cut(s) 2063, 2095
BpmI CTGGAG 4 cut(s) 524, 1194, 1269, 2144
Bpu10I CCTNAGC 1 cut(s) 1124
Bpu14I TTCGAA 1 cut(s) 1340
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 2 cut(s) 280, 1713
BsaBI GATNNNNATC 1 cut(s) 846
BsaHI GRCGYC 1 cut(s) 2194
BsaJI CCNNGG 6 cut(s) 36, 81, 357, 1395, 2223, 2401
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 1418, 1672, 1844
Bse1I ACTGG 8 cut(s) 173, 384, 721, 836, 873, 1211, 1668, 1971
Bse3DI GCAATG 6 cut(s) 327, 498, 1423, 2025, 2157, 2322
Bse8I GATNNNNATC 1 cut(s) 846
BseBI CCWGG 4 cut(s) 1248, 1755, 2054, 2403
BseDI CCNNGG 6 cut(s) 36, 81, 357, 1395, 2223, 2401
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 4 cut(s) 425, 1418, 1672, 1844
BseMI GCAATG 6 cut(s) 327, 498, 1423, 2025, 2157, 2322
BseMII CTCAG 8 cut(s) 38, 468, 606, 1115, 1179, 1809, 1816, 2434
BseNI ACTGG 8 cut(s) 173, 384, 721, 836, 873, 1211, 1668, 1971
BseRI GAGGAG 2 cut(s) 1076, 2122
BseSI GKGCMC 1 cut(s) 740
BseXI GCAGC 4 cut(s) 669, 1007, 1367, 1902
BsgI GTGCAG 3 cut(s) 123, 1366, 1710
Bsh1236I CGCG 2 cut(s) 38, 2484
BshFI GGCC 7 cut(s) 363, 468, 761, 959, 1122, 1525, 1716
BsiHKAI GWGCWC 4 cut(s) 10, 740, 1068, 1130
BsiHKCI CYCGRG 2 cut(s) 470, 2222
BsiSI CCGG 2 cut(s) 279, 1712
BslFI GGGAC 1 cut(s) 1649
BslI CCNNNNNNNGG 4 cut(s) 425, 1418, 1672, 1844
BsmAI GTCTC 5 cut(s) 467, 1805, 1881, 2083, 2274
BsmBI CGTCTC 3 cut(s) 1805, 1881, 2274
BsmFI GGGAC 1 cut(s) 1649
BsmI GAATGC 1 cut(s) 2457
BsnI GGCC 7 cut(s) 363, 468, 761, 959, 1122, 1525, 1716
BsoBI CYCGRG 2 cut(s) 470, 2222
Bsp119I TTCGAA 1 cut(s) 1340
Bsp1286I GDGCHC 4 cut(s) 10, 740, 1068, 1130
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 2 cut(s) 36, 38
BspANI GGCC 7 cut(s) 363, 468, 761, 959, 1122, 1525, 1716
BspCNI CTCAG 8 cut(s) 37, 467, 607, 1116, 1180, 1810, 1815, 2433
BspFNI CGCG 2 cut(s) 38, 2484
BspHI TCATGA 2 cut(s) 892, 1165
BspLI GGNNCC 4 cut(s) 1273, 1401, 2043, 2341
BspMAI CTGCAG 1 cut(s) 997
BspMI ACCTGC 1 cut(s) 1682
BspPI GGATC 9 cut(s) 164, 440, 1266, 1279, 1597, 1862, 1968, 2334, 2347
BspT104I TTCGAA 1 cut(s) 1340
BsrDI GCAATG 6 cut(s) 327, 498, 1423, 2025, 2157, 2322
BsrI ACTGG 8 cut(s) 173, 384, 721, 836, 873, 1211, 1668, 1971
BssECI CCNNGG 6 cut(s) 36, 81, 357, 1395, 2223, 2401
BssNI GRCGYC 1 cut(s) 2194
BssT1I CCWWGG 2 cut(s) 81, 357
Bst2UI CCWGG 4 cut(s) 1248, 1755, 2054, 2403
Bst4CI ACNGT 3 cut(s) 130, 232, 785
Bst6I CTCTTC 2 cut(s) 278, 2272
BstACI GRCGYC 1 cut(s) 2194
BstBI TTCGAA 1 cut(s) 1340
BstDSI CCRYGG 2 cut(s) 36, 357
BstFNI CGCG 2 cut(s) 38, 2484
BstMAI GTCTC 5 cut(s) 467, 1805, 1881, 2083, 2274
BstMWI GCNNNNNNNGC 3 cut(s) 101, 914, 1543
BstNI CCWGG 4 cut(s) 1248, 1755, 2054, 2403
BstNSI RCATGY 1 cut(s) 736
BstPAI GACNNNNGTC 1 cut(s) 1178
BstSCI CCNGG 6 cut(s) 278, 1246, 1711, 1753, 2052, 2401
BstSFI CTRYAG 2 cut(s) 993, 1203
BstSLI GKGCMC 1 cut(s) 740
BstUI CGCG 2 cut(s) 38, 2484
BstV1I GCAGC 4 cut(s) 669, 1007, 1367, 1902
BstV2I GAAGAC 1 cut(s) 1776
BstX2I RGATCY 4 cut(s) 445, 1271, 2298, 2339
BstXI CCANNNNNNTGG 1 cut(s) 1675
BstYI RGATCY 4 cut(s) 445, 1271, 2298, 2339
BsuI GTATCC 1 cut(s) 1254
BsuRI GGCC 7 cut(s) 363, 468, 761, 959, 1122, 1525, 1716
BtgI CCRYGG 2 cut(s) 36, 357
BtgZI GCGATG 1 cut(s) 1998
BtsI GCAGTG 2 cut(s) 111, 2135
BtsIMutI CAGTG 8 cut(s) 32, 111, 516, 790, 880, 960, 1675, 2135
BveI ACCTGC 1 cut(s) 1682
CaiI CAGNNNCTG 2 cut(s) 1001, 1844
CciI TCATGA 2 cut(s) 892, 1165
Cfr13I GGNCC 6 cut(s) 361, 1399, 1523, 1709, 1714, 2041
Cfr42I CCGCGG 1 cut(s) 39
CseI GACGC 1 cut(s) 1787
Csp6I GTAC 4 cut(s) 1008, 1572, 1740, 2024
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviQI GTAC 4 cut(s) 1008, 1572, 1740, 2024
DraI TTTAAA 1 cut(s) 1047
EaeI YGGCCR 1 cut(s) 957
Eam1104I CTCTTC 2 cut(s) 278, 2272
EarI CTCTTC 2 cut(s) 278, 2272
Ecl136II GAGCTC 2 cut(s) 1066, 1128
Eco130I CCWWGG 2 cut(s) 81, 357
Eco147I AGGCCT 1 cut(s) 761
Eco24I GRGCYC 2 cut(s) 1068, 1130
Eco47I GGWCC 3 cut(s) 1399, 1709, 2041
Eco53kI GAGCTC 2 cut(s) 1066, 1128
Eco57I CTGAAG 4 cut(s) 204, 1032, 1476, 1784
Eco88I CYCGRG 2 cut(s) 470, 2222
EcoICRI GAGCTC 2 cut(s) 1066, 1128
EcoO109I RGGNCCY 1 cut(s) 1399
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 4 cut(s) 1246, 1753, 2052, 2401
EcoT14I CCWWGG 2 cut(s) 81, 357
EcoT22I ATGCAT 1 cut(s) 1539
EcoT38I GRGCYC 2 cut(s) 1068, 1130
ErhI CCWWGG 2 cut(s) 81, 357
Esp3I CGTCTC 3 cut(s) 1805, 1881, 2274
FaqI GGGAC 1 cut(s) 1649
FauI CCCGC 1 cut(s) 43
FauNDI CATATG 2 cut(s) 534, 567
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 4 cut(s) 658, 996, 1381, 1916
FriOI GRGCYC 2 cut(s) 1068, 1130
Fsp4HI GCNGC 4 cut(s) 658, 996, 1381, 1916
FspBI CTAG 4 cut(s) 1236, 1485, 1628, 2067
GluI GCNGC 4 cut(s) 658, 996, 1381, 1916
GsuI CTGGAG 4 cut(s) 524, 1194, 1269, 2144
HaeIII GGCC 7 cut(s) 363, 468, 761, 959, 1122, 1525, 1716
HapII CCGG 2 cut(s) 279, 1712
HgaI GACGC 1 cut(s) 1787
Hin1I GRCGYC 1 cut(s) 2194
HincII GTYRAC 1 cut(s) 2263
HindII GTYRAC 1 cut(s) 2263
HpaII CCGG 2 cut(s) 279, 1712
HphI GGTGA 5 cut(s) 762, 1137, 1433, 1654, 1715
Hpy166II GTNNAC 6 cut(s) 137, 699, 738, 1145, 1482, 2263
Hpy8I GTNNAC 6 cut(s) 137, 699, 738, 1145, 1482, 2263
HpyAV CCTTC 7 cut(s) 743, 1007, 1451, 1700, 1759, 1904, 2353
HpyCH4III ACNGT 3 cut(s) 130, 232, 785
HpyCH4IV ACGT 1 cut(s) 2194
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 914, 1543
HpySE526I ACGT 1 cut(s) 2194
Hsp92I GRCGYC 1 cut(s) 2194
Ksp22I TGATCA 1 cut(s) 706
KspI CCGCGG 1 cut(s) 39
LmnI GCTCC 9 cut(s) 19, 543, 1003, 1063, 1133, 1213, 1861, 2125, 2171
Lsp1109I GCAGC 4 cut(s) 669, 1007, 1367, 1902
LweI GCATC 8 cut(s) 178, 478, 925, 1308, 1688, 2089, 2171, 2242
MaeI CTAG 4 cut(s) 1236, 1485, 1628, 2067
MaeII ACGT 1 cut(s) 2194
MaeIII GTNAC 3 cut(s) 87, 311, 1075
MboII GAAGA 9 cut(s) 132, 186, 197, 295, 602, 1154, 1320, 1781, 2289
MflI RGATCY 4 cut(s) 445, 1271, 2298, 2339
MhlI GDGCHC 4 cut(s) 10, 740, 1068, 1130
MlsI TGGCCA 1 cut(s) 959
MluI ACGCGT 1 cut(s) 2482
MluNI TGGCCA 1 cut(s) 959
MlyI GAGTC 3 cut(s) 95, 1214, 2419
Mox20I TGGCCA 1 cut(s) 959
Mph1103I ATGCAT 1 cut(s) 1539
MroXI GAANNNNTTC 1 cut(s) 300
MscI TGGCCA 1 cut(s) 959
MslI CAYNNNNRTG 4 cut(s) 965, 1073, 1316, 1701
Msp20I TGGCCA 1 cut(s) 959
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 2 cut(s) 279, 1712
MspR9I CCNGG 6 cut(s) 280, 1248, 1713, 1755, 2054, 2403
Mva1269I GAATGC 1 cut(s) 2457
MvaI CCWGG 4 cut(s) 1248, 1755, 2054, 2403
MvnI CGCG 2 cut(s) 38, 2484
MwoI GCNNNNNNNGC 3 cut(s) 101, 914, 1543
NciI CCSGG 2 cut(s) 280, 1713
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 534, 567
NlaIV GGNNCC 4 cut(s) 1273, 1401, 2043, 2341
NmuCI GTSAC 2 cut(s) 87, 1075
NsiI ATGCAT 1 cut(s) 1539
NspI RCATGY 1 cut(s) 736
NspV TTCGAA 1 cut(s) 1340
PaeR7I CTCGAG 1 cut(s) 470
PagI TCATGA 2 cut(s) 892, 1165
PceI AGGCCT 1 cut(s) 761
PciI ACATGT 1 cut(s) 732
PctI GAATGC 1 cut(s) 2457
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 7 cut(s) 31, 275, 611, 878, 1724, 1841, 2112
PflFI GACNNNGTC 1 cut(s) 2409
PflMI CCANNNNNTGG 2 cut(s) 1418, 1844
PfoI TCCNGGA 2 cut(s) 278, 1246
PkrI GCNGC 4 cut(s) 659, 997, 1382, 1917
PleI GAGTC 3 cut(s) 94, 1214, 2418
PpsI GAGTC 3 cut(s) 94, 1214, 2418
PpuMI RGGWCCY 1 cut(s) 1399
PscI ACATGT 1 cut(s) 732
PshAI GACNNNNGTC 1 cut(s) 1178
PshBI ATTAAT 1 cut(s) 1101
PsiI TTATAA 1 cut(s) 1809
Psp124BI GAGCTC 2 cut(s) 1068, 1130
Psp5II RGGWCCY 1 cut(s) 1399
Psp6I CCWGG 4 cut(s) 1246, 1753, 2052, 2401
PspGI CCWGG 4 cut(s) 1246, 1753, 2052, 2401
PspN4I GGNNCC 4 cut(s) 1273, 1401, 2043, 2341
PspPI GGNCC 6 cut(s) 361, 1399, 1523, 1709, 1714, 2041
PspPPI RGGWCCY 1 cut(s) 1399
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 2 cut(s) 1001, 1844
PsuI RGATCY 4 cut(s) 445, 1271, 2298, 2339
PsyI GACNNNGTC 1 cut(s) 2409
RsaI GTAC 4 cut(s) 1009, 1573, 1741, 2025
RsaNI GTAC 4 cut(s) 1008, 1572, 1740, 2024
RseI CAYNNNNRTG 4 cut(s) 965, 1073, 1316, 1701
SacI GAGCTC 2 cut(s) 1068, 1130
SacII CCGCGG 1 cut(s) 39
SatI GCNGC 4 cut(s) 658, 996, 1381, 1916
Sau96I GGNCC 6 cut(s) 361, 1399, 1523, 1709, 1714, 2041
SchI GAGTC 3 cut(s) 95, 1214, 2419
ScrFI CCNGG 6 cut(s) 280, 1248, 1713, 1755, 2054, 2403
SduI GDGCHC 4 cut(s) 10, 740, 1068, 1130
SfaNI GCATC 8 cut(s) 178, 478, 925, 1308, 1688, 2089, 2171, 2242
SfcI CTRYAG 2 cut(s) 993, 1203
Sfr274I CTCGAG 1 cut(s) 470
Sfr303I CCGCGG 1 cut(s) 39
SfuI TTCGAA 1 cut(s) 1340
SgrBI CCGCGG 1 cut(s) 39
SinI GGWCC 3 cut(s) 1399, 1709, 2041
SlaI CTCGAG 1 cut(s) 470
SmiMI CAYNNNNRTG 4 cut(s) 965, 1073, 1316, 1701
SmlI CTYRAG 2 cut(s) 53, 470
SmoI CTYRAG 2 cut(s) 53, 470
SpeI ACTAGT 1 cut(s) 1484
SseBI AGGCCT 1 cut(s) 761
SsiI CCGC 2 cut(s) 36, 38
SspMI CTAG 4 cut(s) 1236, 1485, 1628, 2067
SstI GAGCTC 2 cut(s) 1068, 1130
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 6 cut(s) 278, 1246, 1711, 1753, 2052, 2401
StyI CCWWGG 2 cut(s) 81, 357
TaaI ACNGT 3 cut(s) 130, 232, 785
TaiI ACGT 1 cut(s) 2197
TaqI TCGA 5 cut(s) 118, 471, 903, 1340, 1615
TatI WGTACW 2 cut(s) 1007, 1739
TfiI GAWTC 7 cut(s) 31, 275, 611, 878, 1724, 1841, 2112
TscAI CASTG 8 cut(s) 32, 111, 523, 790, 880, 967, 1675, 2135
TseFI GTSAC 2 cut(s) 87, 1075
TseI GCWGC 4 cut(s) 657, 995, 1380, 1915
Tsp45I GTSAC 2 cut(s) 87, 1075
TspGWI ACGGA 3 cut(s) 6, 441, 684
TspRI CASTG 8 cut(s) 32, 111, 523, 790, 880, 967, 1675, 2135
Tth111I GACNNNGTC 1 cut(s) 2409
Van91I CCANNNNNTGG 2 cut(s) 1418, 1844
VneI GTGCAC 1 cut(s) 736
VpaK11BI GGWCC 3 cut(s) 1399, 1709, 2041
VspI ATTAAT 1 cut(s) 1101
XapI RAATTY 3 cut(s) 253, 626, 1646
XceI RCATGY 1 cut(s) 736
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XhoI CTCGAG 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 4 cut(s) 1236, 1485, 1628, 2067
ZraI GACGTC 1 cut(s) 2195
Zsp2I ATGCAT 1 cut(s) 1539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.