Rroxscaffold_7G00166110

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005427
Physical Location & Seq
Reverse (-)
7681513 .. 7686733
5221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00166110.1

Sequence Viewer

Length: 2667 bp
ATGGCCGGGGACGCAGCTGCGGTGCGGAGCTCGGCCGTCGGTGCTTGGATTCCGGCGGACGATGTTTTGCTCAAGAACGCTGTTGAGGCTGGTGCTTCCTTGGAATCACTTGCTAAAGGTGCAGTTCATTTTTCTCGAAGGTTTACTGTGCAAGAACTGCAAGATCGGTGGTACTCTATCCTATACGATCCAGTTGTGGCTGAAGAGGCTTCTGCTCGCATGACTGAGTTTGAGTGTTCCACTACAAATTCCACATCAAAGTTTAGTAGGTTAGGAAGTTCAAAAGAAAACAAATGTGTTCCTGGAAAGAGAAAAGCTGAAAGTGTTCGGAGTAGTTACTATGCTCTGCGTAAAAGGATCTGCAGTGAGCCATTTGACCCCATGGACCTCAATTTTATACTTGCACCCAGTGATAGCAACTATATTGTAAATGGAGATGAGCCTCTATCTGAACATGTCATGACTGGAGATCCAATCCCAAACCATTTTGGAGTTGAGGGATCAGATATTGACAATACTATGCATCATATGTTTCCACAAAATCTGATGCATGACAGCACTGCTATTGGTGGTATTGTTACTGTGGATACATTTCAGATTGGAGGGTTCCAGAAGCCTGCTGAAGAAGATTTTTTAGTTGAGCAAGATAATCTACGTGAAGAAGCTCCTTACATTGAAGACAGTCTGCCTATCACAGGGAATGAATCTGAGGTCAAGGAATTCGGTCAACCAAAGGAATTGCCGGATTGCAGCATGTTCGGTGCTGGTGATCTAGGAATGGAACCTCCTTGTTCACTTGATCAAATTAATAATGACGAGGTAAATATGTGTTCCCCATTTGAAGGGAATCAGGTTTTTAATGTATCTGCTTCAGACAGTAGTGCATCATTTCACGACTTGGAGTATTCAACTCAACTTCCTGGCACACATATCTGGAGCACAGTTCCAGCAACAGCTACACCAGTTGATGTTGGTATCAGAGAGAATGATACGTGTACCAGAGATTCTTTTGAACTTCTTGACGATATTGGTGCTAATAACACTACAATATCAGGGTATGATATTGACTTGAGGACGGAAATGGCAGTCAGTGATTTCAAGAGTCCAGCTAGTACTGAAGTTTATTTGGCAGAATTGTCCAATTCCCTTTTGAACTTTACAAGTGAGGAGGAACTTATGTTCAAAGATGAGATTGATAAGTCTTACTATGATGGCCTGAGCTCACTTTTGATGAGTTCGCCAAAGGATGATGTTGAAGAACACATGATTAATCTAATTGAGCCAGAGACATTGATGGCTCCAGTTATTTACCCTATGAATCCCTCTGGTACAGATCCTTCAGTGGCAGATGACATAAGGGTGTCCCTCCCTGGTGATGATATGAATTGCCATCCAGAGACGCTGATGCAATCATCTCCAACAGCTTTGAACTCTCAGTTTCCCGAATATAAAGATGGAGTTATCTGTTGCACGTTAAATACTGAGGAATGGGAAATTCCATGCAACGATGATGTGTTTCTTCTCAATCATGTGCTATTGTCATCAACTTCCTGTAAAGTAAAAGAAGCCAGTAAGCCAAAGTCGAAATGTTCATCTGTTAAGGATCTTGCAGTTAATCAAAGAAAGGGTGATACGGGCCCCTGTTTTATGCAAAAAGAACAAAGGAATCCTGGAAAATCTCTTGGATCTCAGAGTAAAGGATCACACTGTATACCAGAAGTGAGTTCAAAATCACCTCTGTGTAATTTTGAGTTGTCCAAGACTGAGCCCGTTGATGTGGCTTCTACGAGTACATGTCATGTCCGTGGTAATCGGGGTCAAATTGATTCGGCTAAAGCAATCACAAATCCTCTTCCCGTAATTATGAAAGAAGACACTAGAGATGGTACATTGACAATGTGTTGTGGGTATGCCTCAACAGAGTCTCATATGGCAAACTCATGTGTTGATTACGATTGCGTTAGAAGCTATCCTCAGCCATATGCTACTGAGACTAATTGGGAATCTGATGCATCAGCTACAATTAGAGATCATCAATCATTACATGCAGAAGTTGTGCCAATGGATAATGCTGTCTCAGAACCAGAAGTAAACCCAGTGACATCAGACTTTGAGGGTCTTTTCGATAGTGATGATGATATACCATGTTATTCAGATATAGAGGCAATGATACTTGACATGGATTTGGATCCAGATGACCAAGAAATGTATCATGGTGAGGAAGTCTCAAGATATCAGGATGACGACACTAGGAAAGCAATCATGAGACTGGAGCAGGGTGCTTATTCGTACATGCAAAGAGCCATTGCATCTCACAGAGCATTTGCCATTTTGTATGGGCGTCATTCTAAGCATTATATTAAGAAGCCTGAGGTGTTGCTGGGTAGGACAACAAAAGATGTTGTTGTTGACATTGACTTGGCAAGAGAAGGGCGTGGTAATAAAGTCTCTCGGCAACAGGCAGTATTAAAGATGGAAAAAGATGGATCTTTCCATCTCAAAAATCTTGGGAGTTGTTCAATCTCAGTAAATAGCACAGAATTAACCCCTGGACAGAGTCTATGCCTTAGTTCCAGTTGCTTGATTGAGATAAGGGGAATGCCGTTCATATTTGAGATGAACCAAACTCGTGTAAAGCAATATCTAGATAGCATAACTCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

888

Amino Acids

97.97

Weight (kDa)

4.55

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 16 - 101 2.2e-15 N-terminal region of micro-spherule protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1711
AciI CCGC 3 cut(s) 20, 25, 56
AcoI YGGCCR 2 cut(s) 3, 33
AcsI RAATTY 3 cut(s) 247, 719, 1494
AcuI CTGAAG 5 cut(s) 222, 642, 855, 1137, 1323
AcyI GRCGYC 1 cut(s) 2341
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 7 cut(s) 173, 997, 1114, 1330, 1792, 1888, 2291
AfiI CCNNNNNNNGG 2 cut(s) 695, 842
AflIII ACRYGT 3 cut(s) 454, 992, 1793
AjnI CCWGG 5 cut(s) 301, 919, 1369, 1669, 2548
AleI CACNNNNGTG 1 cut(s) 1738
AloI GAACNNNNNNTCC 2 cut(s) 1163, 1195
Alw21I GWGCWC 3 cut(s) 32, 941, 1223
Alw26I GTCTC 8 cut(s) 1280, 1391, 1929, 1985, 2080, 2230, 2260, 2452
AoxI GGCC 4 cut(s) 3, 33, 1213, 1636
ApaI GGGCCC 1 cut(s) 1640
ApeKI GCWGC 3 cut(s) 14, 17, 750
ApoI RAATTY 3 cut(s) 247, 719, 1494
AseI ATTAAT 2 cut(s) 807, 1269
Asp700I GAANNNNTTC 1 cut(s) 324
AspS9I GGNCC 3 cut(s) 385, 1636, 1637
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 5 cut(s) 779, 1385, 1640, 1725, 2228
AvaII GGWCC 1 cut(s) 385
AxyI CCTNAGG 1 cut(s) 2370
BaeGI GKGCMC 1 cut(s) 1640
BamHI GGATCC 1 cut(s) 2188
BanII GRGCYC 4 cut(s) 32, 1223, 1640, 1770
BarI GAAGNNNNNNTAC 2 cut(s) 1321, 1353
BauI CACGAG 1 cut(s) 2628
BbsI GAAGAC 2 cut(s) 684, 1878
Bbv12I GWGCWC 3 cut(s) 32, 941, 1223
BbvCI CCTCAGC 1 cut(s) 1974
BbvI GCAGC 3 cut(s) 4, 26, 762
BccI CCATC 8 cut(s) 1205, 1288, 1398, 1448, 1877, 2467, 2477, 2502
BceAI ACGGC 2 cut(s) 20, 2587
BcgI CGANNNNNNTGC 4 cut(s) 739, 773, 1013, 1047
BciT130I CCWGG 5 cut(s) 303, 921, 1371, 1671, 2550
BciVI GTATCC 1 cut(s) 580
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 8 cut(s) 1280, 1391, 1929, 1985, 2080, 2230, 2260, 2452
BfaI CTAG 5 cut(s) 773, 1110, 1878, 2250, 2645
BfmI CTRYAG 1 cut(s) 361
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 3 cut(s) 15, 18, 751
BlsI GCNGC 3 cut(s) 16, 19, 752
BmcAI AGTACT 1 cut(s) 1114
Bme1390I CCNGG 6 cut(s) 7, 303, 921, 1371, 1671, 2550
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 3 cut(s) 385, 1636, 1637
BmiI GGNNCC 6 cut(s) 608, 783, 1299, 1638, 1639, 2190
BmrFI CCNGG 6 cut(s) 7, 303, 921, 1371, 1671, 2550
BmrI ACTGGG 2 cut(s) 402, 2090
BmsI GCATC 7 cut(s) 532, 537, 893, 1395, 1999, 2021, 2318
BmuI ACTGGG 2 cut(s) 402, 2090
BpiI GAAGAC 2 cut(s) 684, 1878
BplI GAGNNNNNCTC 2 cut(s) 2210, 2242
BpmI CTGGAG 4 cut(s) 486, 955, 1284, 2291
Bpu10I CCTNAGC 2 cut(s) 1217, 1974
BpuEI CTTGAG 4 cut(s) 56, 1090, 2212, 2643
BpuMI CCSGG 1 cut(s) 7
BsaAI YACGTR 2 cut(s) 656, 993
BsaBI GATNNNNATC 1 cut(s) 2187
BsaHI GRCGYC 1 cut(s) 2341
BsaJI CCNNGG 6 cut(s) 6, 99, 381, 1369, 1804, 2548
Bsc4I CCNNNNNNNGG 2 cut(s) 695, 842
Bse1I ACTGG 9 cut(s) 191, 408, 469, 962, 1301, 1569, 2096, 2274, 2574
Bse21I CCTNAGG 1 cut(s) 2370
Bse3DI GCAATG 2 cut(s) 2172, 2304
Bse8I GATNNNNATC 1 cut(s) 2187
BseBI CCWGG 5 cut(s) 303, 921, 1371, 1671, 2550
BseDI CCNNGG 6 cut(s) 6, 99, 381, 1369, 1804, 2548
BseGI GGATG 3 cut(s) 1252, 1390, 2245
BseJI GATNNNNATC 1 cut(s) 2187
BseLI CCNNNNNNNGG 2 cut(s) 695, 842
BseMI GCAATG 2 cut(s) 2172, 2304
BseNI ACTGG 9 cut(s) 191, 408, 469, 962, 1301, 1569, 2096, 2274, 2574
BseRI GAGGAG 1 cut(s) 1181
BseSI GKGCMC 1 cut(s) 1640
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 3 cut(s) 4, 26, 762
BseYI CCCAGC 1 cut(s) 2380
BsgI GTGCAG 1 cut(s) 141
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 4 cut(s) 5, 35, 1215, 1638
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 3 cut(s) 32, 941, 1223
BsiSI CCGG 3 cut(s) 6, 53, 743
BslFI GGGAC 2 cut(s) 23, 1348
BslI CCNNNNNNNGG 2 cut(s) 695, 842
BsmAI GTCTC 8 cut(s) 1280, 1391, 1929, 1985, 2080, 2230, 2260, 2452
BsmBI CGTCTC 1 cut(s) 1391
BsmFI GGGAC 2 cut(s) 23, 1348
BsmI GAATGC 1 cut(s) 2604
BsnI GGCC 4 cut(s) 5, 35, 1215, 1638
Bsp120I GGGCCC 1 cut(s) 1636
Bsp1286I GDGCHC 5 cut(s) 32, 941, 1223, 1640, 1770
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 3 cut(s) 20, 25, 56
BspANI GGCC 4 cut(s) 5, 35, 1215, 1638
BspHI TCATGA 2 cut(s) 459, 2262
BspLI GGNNCC 6 cut(s) 608, 783, 1299, 1638, 1639, 2190
BspMAI CTGCAG 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 2172, 2304
BsrI ACTGG 9 cut(s) 191, 408, 469, 962, 1301, 1569, 2096, 2274, 2574
BssECI CCNNGG 6 cut(s) 6, 99, 381, 1369, 1804, 2548
BssNAI GTATAC 1 cut(s) 1712
BssNI GRCGYC 1 cut(s) 2341
BssSI CACGAG 1 cut(s) 2628
BssT1I CCWWGG 2 cut(s) 99, 381
Bst1107I GTATAC 1 cut(s) 1712
Bst2BI CACGAG 1 cut(s) 2628
Bst2UI CCWGG 5 cut(s) 303, 921, 1371, 1671, 2550
Bst4CI ACNGT 6 cut(s) 148, 583, 683, 878, 943, 1709
Bst6I CTCTTC 2 cut(s) 198, 1857
BstACI GRCGYC 1 cut(s) 2341
BstAPI GCANNNNNTGC 1 cut(s) 157
BstBAI YACGTR 2 cut(s) 656, 993
BstC8I GCNNGC 2 cut(s) 217, 618
BstDSI CCRYGG 2 cut(s) 381, 1804
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 3 cut(s) 1252, 1390, 2245
BstMAI GTCTC 8 cut(s) 1280, 1391, 1929, 1985, 2080, 2230, 2260, 2452
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 7 cut(s) 11, 41, 86, 119, 157, 206, 1965
BstNI CCWGG 5 cut(s) 303, 921, 1371, 1671, 2550
BstNSI RCATGY 5 cut(s) 458, 757, 1797, 2048, 2296
BstSCI CCNGG 6 cut(s) 5, 301, 919, 1369, 1669, 2548
BstSFI CTRYAG 1 cut(s) 361
BstSLI GKGCMC 1 cut(s) 1640
BstV1I GCAGC 3 cut(s) 4, 26, 762
BstV2I GAAGAC 2 cut(s) 684, 1878
BstX2I RGATCY 7 cut(s) 357, 469, 1333, 1603, 1685, 2188, 2486
BstYI RGATCY 7 cut(s) 357, 469, 1333, 1603, 1685, 2188, 2486
BstZ17I GTATAC 1 cut(s) 1712
BstZI CGGCCG 1 cut(s) 33
Bsu36I CCTNAGG 1 cut(s) 2370
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 4 cut(s) 5, 35, 1215, 1638
BtgI CCRYGG 2 cut(s) 381, 1804
BtsCI GGATG 3 cut(s) 1252, 1390, 2245
BtsI GCAGTG 2 cut(s) 370, 558
BtsIMutI CAGTG 7 cut(s) 370, 415, 558, 1096, 1347, 1705, 2103
Cac8I GCNNGC 2 cut(s) 217, 618
CciI TCATGA 2 cut(s) 459, 2262
Cfr13I GGNCC 3 cut(s) 385, 1636, 1637
CseI GACGC 3 cut(s) 20, 1408, 2330
Csp6I GTAC 7 cut(s) 172, 996, 1113, 1329, 1791, 1887, 2290
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviQI GTAC 7 cut(s) 172, 996, 1113, 1329, 1791, 1887, 2290
DraIII CACNNNGTG 1 cut(s) 410
EaeI YGGCCR 2 cut(s) 3, 33
EagI CGGCCG 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 198, 1857
EarI CTCTTC 2 cut(s) 198, 1857
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 2 cut(s) 30, 1221
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 99, 381
Eco24I GRGCYC 4 cut(s) 32, 1223, 1640, 1770
Eco32I GATATC 1 cut(s) 2234
Eco47I GGWCC 1 cut(s) 385
Eco52I CGGCCG 1 cut(s) 33
Eco53kI GAGCTC 2 cut(s) 30, 1221
Eco57I CTGAAG 5 cut(s) 222, 642, 855, 1137, 1323
Eco81I CCTNAGG 1 cut(s) 2370
EcoICRI GAGCTC 2 cut(s) 30, 1221
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoO109I RGGNCCY 1 cut(s) 1637
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 5 cut(s) 301, 919, 1369, 1669, 2548
EcoRV GATATC 1 cut(s) 2234
EcoT14I CCWWGG 2 cut(s) 99, 381
EcoT22I ATGCAT 3 cut(s) 525, 552, 2014
EcoT38I GRGCYC 4 cut(s) 32, 1223, 1640, 1770
ErhI CCWWGG 2 cut(s) 99, 381
Esp3I CGTCTC 1 cut(s) 1391
FaqI GGGAC 2 cut(s) 23, 1348
FauNDI CATATG 3 cut(s) 528, 1929, 1981
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 1 cut(s) 1711
Fnu4HI GCNGC 3 cut(s) 15, 18, 751
FokI GGATG 3 cut(s) 1259, 1377, 2252
FriOI GRGCYC 4 cut(s) 32, 1223, 1640, 1770
Fsp4HI GCNGC 3 cut(s) 15, 18, 751
FspBI CTAG 5 cut(s) 773, 1110, 1878, 2250, 2645
GluI GCNGC 3 cut(s) 15, 18, 751
GsaI CCCAGC 1 cut(s) 2384
GsuI CTGGAG 4 cut(s) 486, 955, 1284, 2291
HaeIII GGCC 4 cut(s) 5, 35, 1215, 1638
HapII CCGG 3 cut(s) 6, 53, 743
HgaI GACGC 3 cut(s) 20, 1408, 2330
Hin1I GRCGYC 1 cut(s) 2341
HincII GTYRAC 2 cut(s) 728, 2410
HindII GTYRAC 2 cut(s) 728, 2410
HpaII CCGG 3 cut(s) 6, 53, 743
HphI GGTGA 5 cut(s) 779, 1385, 1640, 1725, 2228
Hpy166II GTNNAC 7 cut(s) 144, 728, 794, 996, 1712, 2092, 2410
Hpy8I GTNNAC 7 cut(s) 144, 728, 794, 996, 1712, 2092, 2410
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 132, 836, 1347, 2423
HpyCH4III ACNGT 6 cut(s) 148, 583, 683, 878, 943, 1709
HpyCH4IV ACGT 3 cut(s) 655, 992, 1472
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 41, 86, 119, 157, 206, 1965
HpySE526I ACGT 3 cut(s) 655, 992, 1472
Hsp92I GRCGYC 1 cut(s) 2341
Ksp22I TGATCA 1 cut(s) 799
LmnI GCTCC 5 cut(s) 27, 670, 936, 1303, 2272
Lsp1109I GCAGC 3 cut(s) 4, 26, 762
LweI GCATC 7 cut(s) 532, 537, 893, 1395, 1999, 2021, 2318
MaeI CTAG 5 cut(s) 773, 1110, 1878, 2250, 2645
MaeII ACGT 3 cut(s) 655, 992, 1472
MaeIII GTNAC 3 cut(s) 335, 577, 2098
MboII GAAGA 9 cut(s) 215, 635, 638, 671, 689, 1268, 1511, 1844, 1883
MflI RGATCY 7 cut(s) 357, 469, 1333, 1603, 1685, 2188, 2486
MhlI GDGCHC 5 cut(s) 32, 941, 1223, 1640, 1770
MlyI GAGTC 3 cut(s) 1111, 1931, 2566
MmeI TCCRAC 1 cut(s) 1442
Mph1103I ATGCAT 3 cut(s) 525, 552, 2014
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 9 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2468, 2543
MslI CAYNNNNRTG 3 cut(s) 1358, 1738, 1803
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 6, 53, 743
MspR9I CCNGG 6 cut(s) 7, 303, 921, 1371, 1671, 2550
Mva1269I GAATGC 1 cut(s) 2604
MvaI CCWGG 5 cut(s) 303, 921, 1371, 1671, 2550
MwoI GCNNNNNNNGC 7 cut(s) 11, 41, 86, 119, 157, 206, 1965
NciI CCSGG 1 cut(s) 7
NcoI CCATGG 1 cut(s) 381
NdeI CATATG 3 cut(s) 528, 1929, 1981
NlaIV GGNNCC 6 cut(s) 608, 783, 1299, 1638, 1639, 2190
NmeAIII GCCGAG 2 cut(s) 11, 2431
NmuCI GTSAC 1 cut(s) 2098
NsiI ATGCAT 3 cut(s) 525, 552, 2014
NspI RCATGY 5 cut(s) 458, 757, 1797, 2048, 2296
OliI CACNNNNGTG 1 cut(s) 1738
PagI TCATGA 2 cut(s) 459, 2262
PciI ACATGT 2 cut(s) 454, 1793
PctI GAATGC 1 cut(s) 2604
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 9 cut(s) 49, 104, 704, 847, 1004, 1318, 1666, 1826, 2003
PflFI GACNNNGTC 1 cut(s) 2556
PfoI TCCNGGA 2 cut(s) 301, 1669
PkrI GCNGC 3 cut(s) 16, 19, 752
PleI GAGTC 3 cut(s) 1110, 1930, 2565
PpsI GAGTC 3 cut(s) 1110, 1930, 2565
Ppu21I YACGTR 2 cut(s) 656, 993
PscI ACATGT 2 cut(s) 454, 1793
PshBI ATTAAT 2 cut(s) 807, 1269
Psp124BI GAGCTC 2 cut(s) 32, 1223
Psp6I CCWGG 5 cut(s) 301, 919, 1369, 1669, 2548
PspFI CCCAGC 1 cut(s) 2380
PspGI CCWGG 5 cut(s) 301, 919, 1369, 1669, 2548
PspN4I GGNNCC 6 cut(s) 608, 783, 1299, 1638, 1639, 2190
PspOMI GGGCCC 1 cut(s) 1636
PspPI GGNCC 3 cut(s) 385, 1636, 1637
PstI CTGCAG 1 cut(s) 365
PsuI RGATCY 7 cut(s) 357, 469, 1333, 1603, 1685, 2188, 2486
PsyI GACNNNGTC 1 cut(s) 2556
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 7 cut(s) 173, 997, 1114, 1330, 1792, 1888, 2291
RsaNI GTAC 7 cut(s) 172, 996, 1113, 1329, 1791, 1887, 2290
RseI CAYNNNNRTG 3 cut(s) 1358, 1738, 1803
SacI GAGCTC 2 cut(s) 32, 1223
SaqAI TTAA 9 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2468, 2543
SatI GCNGC 3 cut(s) 15, 18, 751
Sau96I GGNCC 3 cut(s) 385, 1636, 1637
ScaI AGTACT 1 cut(s) 1114
SchI GAGTC 3 cut(s) 1111, 1931, 2566
ScrFI CCNGG 6 cut(s) 7, 303, 921, 1371, 1671, 2550
SduI GDGCHC 5 cut(s) 32, 941, 1223, 1640, 1770
SfaNI GCATC 7 cut(s) 532, 537, 893, 1395, 1999, 2021, 2318
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 3 cut(s) 1358, 1738, 1803
SmlI CTYRAG 4 cut(s) 71, 1069, 2227, 2658
SmoI CTYRAG 4 cut(s) 71, 1069, 2227, 2658
SsiI CCGC 3 cut(s) 20, 25, 56
SspMI CTAG 5 cut(s) 773, 1110, 1878, 2250, 2645
SstI GAGCTC 2 cut(s) 32, 1223
StyD4I CCNGG 6 cut(s) 5, 301, 919, 1369, 1669, 2548
StyI CCWWGG 2 cut(s) 99, 381
TaaI ACNGT 6 cut(s) 148, 583, 683, 878, 943, 1709
TaiI ACGT 3 cut(s) 658, 995, 1475
TaqI TCGA 3 cut(s) 136, 1583, 2124
TaqII GACCGA 1 cut(s) 713
TatI WGTACW 2 cut(s) 1112, 1790
TfiI GAWTC 9 cut(s) 49, 104, 704, 847, 1004, 1318, 1666, 1826, 2003
Tru1I TTAA 9 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2468, 2543
Tru9I TTAA 9 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2468, 2543
TscAI CASTG 7 cut(s) 370, 415, 565, 1096, 1347, 1712, 2103
TseFI GTSAC 1 cut(s) 2098
TseI GCWGC 3 cut(s) 14, 17, 750
Tsp45I GTSAC 1 cut(s) 2098
TspDTI ATGAA 8 cut(s) 116, 717, 1331, 1397, 1581, 1880, 2596, 2633
TspGWI ACGGA 2 cut(s) 1091, 1793
TspRI CASTG 7 cut(s) 370, 415, 565, 1096, 1347, 1712, 2103
Tth111I GACNNNGTC 1 cut(s) 2556
VpaK11BI GGWCC 1 cut(s) 385
VspI ATTAAT 2 cut(s) 807, 1269
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 3 cut(s) 247, 719, 1494
XbaI TCTAGA 1 cut(s) 2644
XceI RCATGY 5 cut(s) 458, 757, 1797, 2048, 2296
XmiI GTMKAC 1 cut(s) 1711
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 5 cut(s) 773, 1110, 1878, 2250, 2645
ZrmI AGTACT 1 cut(s) 1114
Zsp2I ATGCAT 3 cut(s) 525, 552, 2014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.