FvH4_2g38980

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005427
Physical Location & Seq
Forward (+)
28099262 .. 28104803
5542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38980.t2

Sequence Viewer

Length: 2673 bp
ATGGCCGGGGACGCAGCTCTGGCGCGGAGCTCGGCCGTCGGCGCCTGGATTCCGGCCGACGATGTTTTGCTCAAGAACGCCGTTGAGGCTGGTGCTTCCTTGGAATCACTTGCTAAAGGCGCAGTTCATTTTTCTCGAAGGTTTACTGTGCAAGAACTGCAAGATCGGTGGTACTCTATCTTGTATGATCCAGTTGTGGCTGAAGAAGCTTCTGCTCGCATGGCTGAGTTTGAGTTTGCCACTCCAAATTTTGCATCAAAGTTTAGTAGGGTAGGATGTTCAAAAGAAAACAAATGTGCACCTGGAAAGAGAAAAGCTGAAAGTGTTCGGAGTAGTTACTCTGCTCTGCGTAAAAGGATCTGCAGCGAGCCATTTGACTCCATGGACCTCAGTTTTATACTTGCACCCAGCGATAGTAACTATATTGTAAATGGAGATGAGCCTCTCTCTGAACATGTCATGACTGGAGATCCAATCGCACATAATTTTGGACTTGACGGATCAGATATGGATAATACTATGCATCAGACATTTCCACAAGATCTGATGCATGACAGCACTGTAATTGGTGGTATTGAAACTGCGGATACATTTGGAGGACTGCAGAAGCCCACTGAAGAAGATTTTTTAGTTGAACAAGATAATCTACATGAAGAAGTTCCCTATATCGAAGACAATCTGCCAATCACAGGGAATGGATCTGAGGTCAATGAATTCGGTCAACCAAAGGATTTGCTGGATTGCAGCATGTTCAGTGCCGGTAATTCAGGAATGGAACCTCCGTGTTCGCTTGATCAAATTAATAATGACGGGGTACATTTGTGTTCCCCATTTGAAGGGAATCAGGTTTTAAATGTAACTGCTTCAGAAAGCACTGCATCATTTCATGACTTGGAGTATTCAACTCAACTTCCTGAATCACATATCTGGAGCACAGTTCCAGCAACATCCGTGCCGGTTGATATTGGCCCCAGAGAGAATGATACTTGTACAAGAGATTCTTTTGAACTTCTTGACGTTATTGGTGCCAAGAACACTACAATATCAGGATACAATGTTGACCTGGGGCCAGAAGTCAAAGTCAGTGATTTCAAGAGTCCGGCTATTACTGATGTTTATTTGGCAGAATTGTCCAATTCCCTTTTAAACTTTACAAATGAGGAGGAACTCATGTTCAAAGATGAGATCGATAAGTCATACTATGATGGCCTGAGCTCACTTTTGATGAGTTCTCCAAAGGATGATATTGAAGAACACATGATTAATCGAATTGAGCCTGGGACATCTATGGCTCCACCTATGTACCCTATGGATCCCTCTTGTATAGGTCCTGCACTGGCAGATGACATAAGGGGGTCCCTCCCTGATGATGATATGAATTGCCATCCAGAGATGCTGATGCAATCATCTCCAACAGCTTCAAACTCTCAGTTTCCTGAATATAAAGATGGAGTTATCTGTTGCACGTTGAATACTGAGGAATGGGAAATTCCATGCAACGATGATGTGATTTTTCACAATCATGTGCCATTGTCATCAACTCACTGTAAAGTAAAAGAAGACAGTGAGCCAAAGCCGATATGTGCATCTGTTAAGGATCTTACTGTTAATCAAAGAAAGGGTGATACTAGAAGAAAGGTCCCCAGCTTAATGCCAAAAGAACAAAGGAATCCTGGAAAATCACACTGTATACTAGAAGTGAGTTCAAAACAACCTCTGAGTGATTTTGAGCCGTCCAAGACTAACCCGGTTGATGTGGCTTCTACAAGTACATGTCATGTCAGTGGTAATTGGGGTCAAATTGACTCGGCTAAAGCAATCACAAATCCTCTTCCCGGAATTATGGAGGAAGACAGTACATTGACAAACTGTCTTGGGTATACCTCAACAGAGTCTCATATGGCTCACCCACATCTTGATTACGATTGCGTTAGAAGCTATCCTGAGCCATATGCTACTGAAACTGATTGGGAACCTGATGCATCAGCTACAATTAGAGATCATCCATCATTACTTGCAGAAGTTGCGCCTATGGATGATGCTGTCTTAGAACCAGAAGTAAACCCAATGACATCAGATTTTGAGGGTCTTTTAGAGAGTGATGATGATATACCATGTTATTCAGATATAGAGGCAATGATACTTGACATGGATTTGGATCCAGATGATCAAGAAATGTATTCTGGTGAGGAAGTCTCAAGATACCAGGATGAGGACACTAAGAGAGCAATCATGAGACTGGAGCAGGGTGCTTATTCGTATATGCAGAGAGCCATTGCATCTCACGGAGCATTTGCCATTTTGTATGGCCGCCATTCAAAGCATTTTATTAAGAAGCCTGAGGTGTTACTGGGTAGGACAACAAACGATCTTATTGTTGACATTGACTTGGCAAGAGAAGGGAGTGGTAATAAAGTCTCTCGGCAACAGGCAACATTAAAGATGGAAAAAGATGGATCCTTCTATCTGAAAAATCTTGGGAGTTGTTCAATCTCAGTAAATAGCACGGAATTAACCCCAGGACAGAGTCTACGCCTTAGTTCCAGTTGCTTGATTGAGATACGCGGAATGCCATTCATATTTGAAGTGAACGAAATTCGTGTGAAGCAGTATCTAGATAGCATAACTCAAGCCTCAAGGTTAGCACAAGACAACATCTGCGTAGGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

891

Amino Acids

98.1

Weight (kDa)

4.46

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 16 - 78 5.4e-14 N-terminal region of micro-spherule protein
FHA PF00498 780 - 851 2.6e-07 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 41, 1025
AccI GTMKAC 3 cut(s) 1690, 1880, 2530
AccII CGCG 2 cut(s) 25, 2565
AciI CCGC 4 cut(s) 25, 584, 2311, 2565
AcoI YGGCCR 4 cut(s) 3, 33, 54, 2308
AcsI RAATTY 4 cut(s) 247, 713, 1488, 2595
AcuI CTGAAG 3 cut(s) 222, 636, 849
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 6 cut(s) 173, 816, 991, 1304, 1771, 1858
AfiI CCNNNNNNNGG 4 cut(s) 689, 836, 1835, 2212
AflIII ACRYGT 2 cut(s) 454, 1772
AhdI GACNNNNNGTC 1 cut(s) 1869
AjnI CCWGG 7 cut(s) 44, 301, 1062, 1276, 1672, 2205, 2518
AloI GAACNNNNNNTCC 2 cut(s) 1157, 1189
AluBI AGCT 9 cut(s) 17, 30, 209, 317, 1215, 1418, 1647, 1938, 1988
AluI AGCT 9 cut(s) 17, 30, 209, 317, 1215, 1418, 1647, 1938, 1988
Alw21I GWGCWC 4 cut(s) 32, 301, 935, 1217
Alw26I GTCTC 4 cut(s) 1899, 2200, 2230, 2422
Alw44I GTGCAC 1 cut(s) 297
AoxI GGCC 7 cut(s) 3, 33, 54, 967, 1067, 1207, 2308
ApaLI GTGCAC 1 cut(s) 297
ApeKI GCWGC 3 cut(s) 14, 363, 744
ApoI RAATTY 4 cut(s) 247, 713, 1488, 2595
AseI ATTAAT 2 cut(s) 801, 1263
Asp700I GAANNNNTTC 2 cut(s) 324, 657
AspLEI GCGC 4 cut(s) 25, 44, 122, 2029
AspS9I GGNCC 6 cut(s) 385, 968, 1067, 1328, 1356, 1639
AsuC2I CCSGG 3 cut(s) 7, 1748, 1836
AsuHPI GGTGA 3 cut(s) 1634, 1898, 2198
AvaII GGWCC 4 cut(s) 385, 1328, 1356, 1639
AxyI CCTNAGG 1 cut(s) 2340
BaeGI GKGCMC 1 cut(s) 301
BaeI ACNNNNGTAYC 4 cut(s) 806, 839, 1286, 1319
BamHI GGATCC 3 cut(s) 1312, 2158, 2456
BanI GGYRCC 2 cut(s) 41, 1025
BanII GRGCYC 2 cut(s) 32, 1217
BbsI GAAGAC 3 cut(s) 678, 1566, 1857
Bbv12I GWGCWC 4 cut(s) 32, 301, 935, 1217
BbvI GCAGC 3 cut(s) 26, 375, 756
BccI CCATC 6 cut(s) 1199, 1392, 1442, 2014, 2437, 2447
BceAI ACGGC 3 cut(s) 20, 65, 1717
BciT130I CCWGG 7 cut(s) 46, 303, 1064, 1278, 1674, 2207, 2520
BciVI GTATCC 2 cut(s) 580, 1043
BclI TGATCA 2 cut(s) 793, 2167
BcnI CCSGG 3 cut(s) 7, 1748, 1836
BcoDI GTCTC 4 cut(s) 1899, 2200, 2230, 2422
BfaI CTAG 3 cut(s) 1629, 1694, 2615
BfmI CTRYAG 2 cut(s) 361, 602
BfoI RGCGCY 1 cut(s) 45
BfuI GTATCC 2 cut(s) 580, 1043
BglI GCCNNNNNGGC 1 cut(s) 86
BglII AGATCT 1 cut(s) 541
BisI GCNGC 4 cut(s) 15, 364, 745, 2311
BlsI GCNGC 4 cut(s) 16, 365, 746, 2312
Bme18I GGWCC 4 cut(s) 385, 1328, 1356, 1639
BmeRI GACNNNNNGTC 1 cut(s) 1869
BmgT120I GGNCC 6 cut(s) 385, 968, 1067, 1328, 1356, 1639
BmrI ACTGGG 1 cut(s) 2360
BmuI ACTGGG 1 cut(s) 2360
BpiI GAAGAC 3 cut(s) 678, 1566, 1857
BplI GAGNNNNNCTC 4 cut(s) 431, 463, 2180, 2212
BpmI CTGGAG 3 cut(s) 486, 949, 2261
Bpu10I CCTNAGC 2 cut(s) 1211, 1944
BpuEI CTTGAG 4 cut(s) 56, 2182, 2613, 2620
BpuMI CCSGG 3 cut(s) 7, 1748, 1836
Bsa29I ATCGAT 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 2157
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 6 cut(s) 6, 99, 381, 1063, 1277, 2518
Bsc4I CCNNNNNNNGG 4 cut(s) 689, 836, 1835, 2212
Bse118I RCCGGY 2 cut(s) 758, 955
Bse1I ACTGG 6 cut(s) 191, 469, 1341, 2244, 2355, 2544
Bse21I CCTNAGG 1 cut(s) 2340
Bse3DI GCAATG 2 cut(s) 2142, 2274
Bse8I GATNNNNATC 1 cut(s) 2157
BseBI CCWGG 7 cut(s) 46, 303, 1064, 1278, 1674, 2207, 2520
BseCI ATCGAT 1 cut(s) 1188
BseDI CCNNGG 6 cut(s) 6, 99, 381, 1063, 1277, 2518
BseGI GGATG 7 cut(s) 281, 947, 1246, 1384, 2002, 2041, 2215
BseJI GATNNNNATC 1 cut(s) 2157
BseLI CCNNNNNNNGG 4 cut(s) 689, 836, 1835, 2212
BseMI GCAATG 2 cut(s) 2142, 2274
BseNI ACTGG 6 cut(s) 191, 469, 1341, 2244, 2355, 2544
BseRI GAGGAG 1 cut(s) 1175
BseSI GKGCMC 1 cut(s) 301
BseX3I CGGCCG 2 cut(s) 33, 54
BseXI GCAGC 3 cut(s) 26, 375, 756
BseYI CCCAGC 2 cut(s) 407, 1643
BsgI GTGCAG 1 cut(s) 1317
Bsh1236I CGCG 2 cut(s) 25, 2565
Bsh1285I CGRYCG 2 cut(s) 36, 57
BshFI GGCC 7 cut(s) 5, 35, 56, 969, 1069, 1209, 2310
BshNI GGYRCC 2 cut(s) 41, 1025
BshVI ATCGAT 1 cut(s) 1188
BsiEI CGRYCG 2 cut(s) 36, 57
BsiHKAI GWGCWC 4 cut(s) 32, 301, 935, 1217
BsiSI CCGG 7 cut(s) 6, 53, 759, 956, 1100, 1748, 1836
BslFI GGGAC 4 cut(s) 23, 1294, 1342, 1625
BslI CCNNNNNNNGG 4 cut(s) 689, 836, 1835, 2212
BsmAI GTCTC 4 cut(s) 1899, 2200, 2230, 2422
BsmFI GGGAC 4 cut(s) 23, 1294, 1342, 1625
BsmI GAATGC 1 cut(s) 2574
BsnI GGCC 7 cut(s) 5, 35, 56, 969, 1069, 1209, 2310
Bsp1286I GDGCHC 4 cut(s) 32, 301, 935, 1217
Bsp1407I TGTACA 1 cut(s) 989
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 4 cut(s) 25, 584, 2311, 2565
BspANI GGCC 7 cut(s) 5, 35, 56, 969, 1069, 1209, 2310
BspDI ATCGAT 1 cut(s) 1188
BspFNI CGCG 2 cut(s) 25, 2565
BspHI TCATGA 3 cut(s) 459, 886, 2232
BspMAI CTGCAG 2 cut(s) 365, 606
BspT107I GGYRCC 2 cut(s) 41, 1025
BsrDI GCAATG 2 cut(s) 2142, 2274
BsrFI RCCGGY 2 cut(s) 758, 955
BsrGI TGTACA 1 cut(s) 989
BsrI ACTGG 6 cut(s) 191, 469, 1341, 2244, 2355, 2544
BssAI RCCGGY 2 cut(s) 758, 955
BssECI CCNNGG 6 cut(s) 6, 99, 381, 1063, 1277, 2518
BssNAI GTATAC 2 cut(s) 1691, 1881
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 99, 381
Bst1107I GTATAC 2 cut(s) 1691, 1881
Bst2UI CCWGG 7 cut(s) 46, 303, 1064, 1278, 1674, 2207, 2520
Bst4CI ACNGT 9 cut(s) 148, 562, 937, 1547, 1565, 1606, 1688, 1856, 1871
Bst6I CTCTTC 1 cut(s) 1836
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 157, 2024
BstAUI TGTACA 1 cut(s) 989
BstC8I GCNNGC 2 cut(s) 217, 368
BstDSI CCRYGG 1 cut(s) 381
BstF5I GGATG 7 cut(s) 281, 947, 1246, 1384, 2002, 2041, 2215
BstFNI CGCG 2 cut(s) 25, 2565
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 4 cut(s) 25, 44, 122, 2029
BstMAI GTCTC 4 cut(s) 1899, 2200, 2230, 2422
BstMCI CGRYCG 2 cut(s) 36, 57
BstNI CCWGG 7 cut(s) 46, 303, 1064, 1278, 1674, 2207, 2520
BstNSI RCATGY 3 cut(s) 458, 751, 1776
BstSFI CTRYAG 2 cut(s) 361, 602
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 2 cut(s) 25, 2565
BstV1I GCAGC 3 cut(s) 26, 375, 756
BstV2I GAAGAC 3 cut(s) 678, 1566, 1857
BstX2I RGATCY 8 cut(s) 357, 469, 541, 698, 1312, 1597, 2158, 2456
BstYI RGATCY 8 cut(s) 357, 469, 541, 698, 1312, 1597, 2158, 2456
BstZ17I GTATAC 2 cut(s) 1691, 1881
BstZI CGGCCG 2 cut(s) 33, 54
Bsu15I ATCGAT 1 cut(s) 1188
Bsu36I CCTNAGG 1 cut(s) 2340
BsuI GTATCC 2 cut(s) 580, 1043
BsuRI GGCC 7 cut(s) 5, 35, 56, 969, 1069, 1209, 2310
BsuTUI ATCGAT 1 cut(s) 1188
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 7 cut(s) 281, 947, 1246, 1384, 2002, 2041, 2215
BtsI GCAGTG 1 cut(s) 873
Cac8I GCNNGC 2 cut(s) 217, 368
CciI TCATGA 3 cut(s) 459, 886, 2232
CfoI GCGC 4 cut(s) 25, 44, 122, 2029
Cfr10I RCCGGY 2 cut(s) 758, 955
Cfr13I GGNCC 6 cut(s) 385, 968, 1067, 1328, 1356, 1639
ClaI ATCGAT 1 cut(s) 1188
CseI GACGC 1 cut(s) 20
Csp6I GTAC 6 cut(s) 172, 815, 990, 1303, 1770, 1857
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviQI GTAC 6 cut(s) 172, 815, 990, 1303, 1770, 1857
DinI GGCGCC 1 cut(s) 43
DraI TTTAAA 2 cut(s) 852, 1146
DriI GACNNNNNGTC 1 cut(s) 1869
EaeI YGGCCR 4 cut(s) 3, 33, 54, 2308
EagI CGGCCG 2 cut(s) 33, 54
Eam1104I CTCTTC 1 cut(s) 1836
Eam1105I GACNNNNNGTC 1 cut(s) 1869
EarI CTCTTC 1 cut(s) 1836
Ecl136II GAGCTC 2 cut(s) 30, 1215
EclXI CGGCCG 2 cut(s) 33, 54
Eco130I CCWWGG 2 cut(s) 99, 381
Eco24I GRGCYC 2 cut(s) 32, 1217
Eco47I GGWCC 4 cut(s) 385, 1328, 1356, 1639
Eco52I CGGCCG 2 cut(s) 33, 54
Eco53kI GAGCTC 2 cut(s) 30, 1215
Eco57I CTGAAG 3 cut(s) 222, 636, 849
Eco81I CCTNAGG 1 cut(s) 2340
EcoICRI GAGCTC 2 cut(s) 30, 1215
EcoO109I RGGNCCY 3 cut(s) 1328, 1356, 1639
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 7 cut(s) 44, 301, 1062, 1276, 1672, 2205, 2518
EcoT14I CCWWGG 2 cut(s) 99, 381
EcoT22I ATGCAT 3 cut(s) 525, 552, 1984
EcoT38I GRGCYC 2 cut(s) 32, 1217
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 99, 381
FalI AAGNNNNNCTT 2 cut(s) 985, 1017
FaqI GGGAC 4 cut(s) 23, 1294, 1342, 1625
FauNDI CATATG 2 cut(s) 1899, 1951
FbaI TGATCA 2 cut(s) 793, 2167
FblI GTMKAC 3 cut(s) 1690, 1880, 2530
Fnu4HI GCNGC 4 cut(s) 15, 364, 745, 2311
FokI GGATG 7 cut(s) 288, 934, 1253, 1371, 1989, 2048, 2222
FriOI GRGCYC 2 cut(s) 32, 1217
Fsp4HI GCNGC 4 cut(s) 15, 364, 745, 2311
FspBI CTAG 3 cut(s) 1629, 1694, 2615
GlaI GCGC 4 cut(s) 24, 43, 121, 2028
GluI GCNGC 4 cut(s) 15, 364, 745, 2311
GsaI CCCAGC 2 cut(s) 411, 1647
GsuI CTGGAG 3 cut(s) 486, 949, 2261
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 7 cut(s) 5, 35, 56, 969, 1069, 1209, 2310
HapII CCGG 7 cut(s) 6, 53, 759, 956, 1100, 1748, 1836
HgaI GACGC 1 cut(s) 20
HhaI GCGC 4 cut(s) 25, 44, 122, 2029
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 4 cut(s) 23, 42, 120, 2027
HinP1I GCGC 4 cut(s) 23, 42, 120, 2027
HincII GTYRAC 3 cut(s) 722, 1060, 2380
HindII GTYRAC 3 cut(s) 722, 1060, 2380
HindIII AAGCTT 1 cut(s) 207
HpaII CCGG 7 cut(s) 6, 53, 759, 956, 1100, 1748, 1836
HphI GGTGA 3 cut(s) 1634, 1898, 2198
Hpy99I CGWCG 2 cut(s) 41, 62
HpyAV CCTTC 4 cut(s) 132, 830, 2393, 2470
HpyCH4III ACNGT 9 cut(s) 148, 562, 937, 1547, 1565, 1606, 1688, 1856, 1871
HpyCH4IV ACGT 2 cut(s) 1017, 1466
HpySE526I ACGT 2 cut(s) 1017, 1466
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 4 cut(s) 23, 42, 120, 2027
KasI GGCGCC 1 cut(s) 41
KflI GGGWCCC 1 cut(s) 1356
Ksp22I TGATCA 2 cut(s) 793, 2167
LmnI GCTCC 5 cut(s) 27, 930, 1297, 2242, 2288
Lsp1109I GCAGC 3 cut(s) 26, 375, 756
MaeI CTAG 3 cut(s) 1629, 1694, 2615
MaeII ACGT 2 cut(s) 1017, 1466
MaeIII GTNAC 4 cut(s) 335, 416, 856, 2346
MflI RGATCY 8 cut(s) 357, 469, 541, 698, 1312, 1597, 2158, 2456
MhlI GDGCHC 4 cut(s) 32, 301, 935, 1217
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 5 cut(s) 371, 1105, 1799, 1901, 2536
MmeI TCCRAC 1 cut(s) 1436
Mph1103I ATGCAT 3 cut(s) 525, 552, 1984
MroXI GAANNNNTTC 2 cut(s) 324, 657
MslI CAYNNNNRTG 2 cut(s) 1521, 1782
MspI CCGG 7 cut(s) 6, 53, 759, 956, 1100, 1748, 1836
Mva1269I GAATGC 1 cut(s) 2574
MvaI CCWGG 7 cut(s) 46, 303, 1064, 1278, 1674, 2207, 2520
MvnI CGCG 2 cut(s) 25, 2565
NarI GGCGCC 1 cut(s) 42
NciI CCSGG 3 cut(s) 7, 1748, 1836
NcoI CCATGG 1 cut(s) 381
NdeI CATATG 2 cut(s) 1899, 1951
NmeAIII GCCGAG 3 cut(s) 11, 1787, 2401
NsiI ATGCAT 3 cut(s) 525, 552, 1984
NspI RCATGY 3 cut(s) 458, 751, 1776
PagI TCATGA 3 cut(s) 459, 886, 2232
PciI ACATGT 2 cut(s) 454, 1772
PctI GAATGC 1 cut(s) 2574
PdmI GAANNNNTTC 2 cut(s) 324, 657
PfeI GAWTC 6 cut(s) 49, 104, 841, 917, 998, 1669
PflFI GACNNNGTC 1 cut(s) 2526
PfoI TCCNGGA 2 cut(s) 1672, 1834
PkrI GCNGC 4 cut(s) 16, 365, 746, 2312
PleI GAGTC 5 cut(s) 371, 1104, 1799, 1900, 2535
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 5 cut(s) 371, 1104, 1799, 1900, 2535
PpuMI RGGWCCY 3 cut(s) 1328, 1356, 1639
PscI ACATGT 2 cut(s) 454, 1772
PshBI ATTAAT 2 cut(s) 801, 1263
Psp124BI GAGCTC 2 cut(s) 32, 1217
Psp5II RGGWCCY 3 cut(s) 1328, 1356, 1639
Psp6I CCWGG 7 cut(s) 44, 301, 1062, 1276, 1672, 2205, 2518
PspFI CCCAGC 2 cut(s) 407, 1643
PspGI CCWGG 7 cut(s) 44, 301, 1062, 1276, 1672, 2205, 2518
PspPI GGNCC 6 cut(s) 385, 968, 1067, 1328, 1356, 1639
PspPPI RGGWCCY 3 cut(s) 1328, 1356, 1639
PstI CTGCAG 2 cut(s) 365, 606
PsuI RGATCY 8 cut(s) 357, 469, 541, 698, 1312, 1597, 2158, 2456
PsyI GACNNNGTC 1 cut(s) 2526
RsaI GTAC 6 cut(s) 173, 816, 991, 1304, 1771, 1858
RsaNI GTAC 6 cut(s) 172, 815, 990, 1303, 1770, 1857
RseI CAYNNNNRTG 2 cut(s) 1521, 1782
SacI GAGCTC 2 cut(s) 32, 1217
SatI GCNGC 4 cut(s) 15, 364, 745, 2311
Sau96I GGNCC 6 cut(s) 385, 968, 1067, 1328, 1356, 1639
SchI GAGTC 5 cut(s) 371, 1105, 1799, 1901, 2536
SduI GDGCHC 4 cut(s) 32, 301, 935, 1217
SfcI CTRYAG 2 cut(s) 361, 602
SfoI GGCGCC 1 cut(s) 43
SinI GGWCC 4 cut(s) 385, 1328, 1356, 1639
SmiMI CAYNNNNRTG 2 cut(s) 1521, 1782
SmlI CTYRAG 4 cut(s) 71, 2197, 2628, 2635
SmoI CTYRAG 4 cut(s) 71, 2197, 2628, 2635
SsiI CCGC 4 cut(s) 25, 584, 2311, 2565
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 3 cut(s) 1629, 1694, 2615
SstI GAGCTC 2 cut(s) 32, 1217
StyI CCWWGG 2 cut(s) 99, 381
TaaI ACNGT 9 cut(s) 148, 562, 937, 1547, 1565, 1606, 1688, 1856, 1871
TaiI ACGT 2 cut(s) 1020, 1469
TaqI TCGA 4 cut(s) 136, 669, 1188, 1267
TaqII GACCGA 1 cut(s) 707
TatI WGTACW 3 cut(s) 989, 1769, 1856
TauI GCSGC 1 cut(s) 2313
TfiI GAWTC 6 cut(s) 49, 104, 841, 917, 998, 1669
TseI GCWGC 3 cut(s) 14, 363, 744
TspDTI ATGAA 6 cut(s) 116, 666, 726, 875, 1391, 2566
TspGWI ACGGA 5 cut(s) 513, 771, 940, 2301, 2522
Tth111I GACNNNGTC 1 cut(s) 2526
VneI GTGCAC 1 cut(s) 297
VpaK11BI GGWCC 4 cut(s) 385, 1328, 1356, 1639
VspI ATTAAT 2 cut(s) 801, 1263
XapI RAATTY 4 cut(s) 247, 713, 1488, 2595
XbaI TCTAGA 1 cut(s) 2614
XceI RCATGY 3 cut(s) 458, 751, 1776
XmiI GTMKAC 3 cut(s) 1690, 1880, 2530
XmnI GAANNNNTTC 2 cut(s) 324, 657
XspI CTAG 3 cut(s) 1629, 1694, 2615
Zsp2I ATGCAT 3 cut(s) 525, 552, 1984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.