MD08G1021200.v1.1

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005427
Physical Location & Seq
Reverse (-)
1528192 .. 1533353
5162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1021200.v1.1.491

Sequence Viewer

Length: 2502 bp
ATGGGTGCTCTCGCTCCGTTCTCTAAATGGATCCCGGCGGATGATATTCTGCTCAAAAAAGCTGTTGAGGATGGTGCTTCGTTGGAGTCGCTTGCTAAAGGTGCAGTGCATTTTTCTCAAAGATTTACTGTTCGTGAGCTGCAAGATCGGTGGTATTCTCTCCTGTATGATCCAGTAGTTTCTGAAGATGCTTCTGCTCGCATGATTGAGTTTGAGAGTTCCACCACGGCTGTTCCATTTGACAGGTCGGGAAATTCAAAAGAAAAAAAATGTGAATCCGGAAAGAGAAAAGCTGAAAGTGTTCGTAGTAGTTACTATGCAATGCGTAAAAGGATTTGCAATGAGCCATTTAATTCCATGGGCCTTAATTTTCTTGTTGCACCCAGTAATAACAACTATCATGGAAATGGAGACGAGCCTCTTTATGGAAATTGCATAAATGGTGATCCGATGTCAGCCCCTTTTGGCCTTGAGACGTCGGATATGGATGCTATGCAAAATCTGATGAATGTCGGCATTGATGATACCTTTCATATGCTCCAGAACCCAGATGGGAATGATTTTCATATGGAACAAGGTAACATACATGAAGATATTATGACATGGAATGAATCTGAGGTGGATGAATTCAATCATCCAGAGGGATTGCCAGATTGCAGCCTGTTCAATGCTGATGGCTTGGGAATGAAATTTACACTTGATCAAATTAATGGCAACGAGGGAAATATGTGCACAGAATTTGAAGGGAACAACGCCTTTAATTCACCTGTTTCAGACGGTGGTGCATTGTTTAACAACTTGGAGTATTCATCTCCACTTCCAGCTAAGCCAGTCGATGTTGATCTTAGTGAAAATAATACATGCACCGGCGATGTTTTTGAACTTCATGAGAATATCGATGCTGACAATGCAAGAACATCAGAGACTGAAGTTCATGTTAGGATTGAGGTGGATGCTGAAATGCCATGTGGTGATTTCCAGAGTTCAGCAGCTCCTGTTAGTACTGAAGGTTATTTGGCAGAACTGTCCAATTCGCTTTTAAACTTTACGAACGAGGAGCTCATGCTCATGACTGCTGATGGAAAAGATGTGATTGATAAGTCTTACTATGATGGCTTGAGCTCCCTTCTGTTGAGGTCACCGAAAGATGGTGTTCATCAAGAACAGACGATCGTCAAATCTGAGCCAGAAACATCTGTAGCTCCAGTTATGGACTCCATGAATCCTTCTAGTACATATCTTGGAGTGGTAGATGATAATAAAGGATCCCCAAATGCTGATGAAGATATGGTTTTCCATTCAGAGACTCTGATGCTATCATCTTCAACTGCTTCGAACTATCAAAATCCTGAATTGAAAGATGGAGTTATCTGCTGCACATTAAACACCGAGGACCCAGAAATTCCATGCAATGATGATATCTTTCTACCAAATCGTCCATCAACTTCTGAAGTGAATAAGCCATTATCTTTGTCCACTAGAGGTTTCCCTGTTAATAAGAGAAATAATGCCACAGGGTCATGCTTTATGCACAGAGAGCGAAAAAATCTAGGAGAACCGCATAGTACCTCTCAGATTAAAGGATCACACTTCTTACAAGAAAAGGGTCTAAAACCTCCACTAGGTAATTTCGGGGTGAAATTTGAGCTGTCTAAGACTGACGCCATTCAAGTGGCATCTAAGAATGCAGGTCATGTTAGTGAAGGTCTGGATCAAATTTATTCAGCCAATCCAAGTACAAGTGTTCAACCTGGAATCCTGAAGGAAGACACTAGAGAAAGTATATCGGCAAAGCTTCTCAATTACAACTCAACTGAGTTTCATGTGGAGAACCCAGATTTGGGTGTCATTAAACAGGAGCTTGATCCATCAGCTACAATTAGAGACAATAAATCATTACATACAGAAGGGATTGCTGAAGCACAATTAAACCCCGAGACATCAGATCAAATGGGGCTTTTCGAGAGCGATGATGATGTACCATGTTATTCCGATATAGAGGCAATGGTACTTGATATGGATTTGGACCCAGATGACCAGGAGTTGTATTCTAGCGAGGAAGTCTCAAAATATCAGAATGAGAATGCTAAGAGGAGAATCATAAGGCTGGAGCAGTGTGCTTATTCATATTTGCAAAGAGCCATTGCAACTCACGGAGCATTTGCCATTTTGTATGGGCGTCATTCTAAGCATTACATTAAGAAGCCTGAGGTTTTATTGGGTAGAGCAACAGACGATTCTGTTGTTGATATTGACTTGGGAAGAGAAGGGCGTGGTAACAAGATATCTCGAAAACAGGCAATGATAAATATGGACAAAGATGGATCCTTCCGTCTGAAAAATCTTGGGAAGTGTCCAATCTCGGTAAATAGCAAGGAAGTAGCCCCTGGACAAAGTCTAAGCCTTAGTTCAAGTTGCTTGATCGAGATACGGGGAATGCCATTCATATTCGAGATGAACCAAACACGCGTGAAGCAGTATTTGGATAGCATCCCGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

834

Amino Acids

92.09

Weight (kDa)

4.64

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 9 - 75 6.6e-15 N-terminal region of micro-spherule protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 1679
AccII CGCG 1 cut(s) 2469
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 3 cut(s) 38, 1559, 2495
AcsI RAATTY 7 cut(s) 253, 626, 689, 737, 1401, 1640, 1716
AcuI CTGAAG 6 cut(s) 204, 948, 1026, 1470, 1781, 1938
AcyI GRCGYC 3 cut(s) 476, 1662, 2179
AfaI GTAC 6 cut(s) 1003, 1234, 1567, 1738, 1980, 2010
AfiI CCNNNNNNNGG 5 cut(s) 425, 1148, 1622, 1840, 1841
AflIII ACRYGT 1 cut(s) 2467
AjnI CCWGG 3 cut(s) 1750, 2037, 2386
AjuI GAANNNNNNNTTGG 4 cut(s) 1823, 1855, 2465, 2497
Alw21I GWGCWC 4 cut(s) 10, 734, 1062, 1124
Alw26I GTCTC 7 cut(s) 405, 467, 917, 1298, 1878, 1931, 2068
Alw44I GTGCAC 1 cut(s) 730
AlwNI CAGNNNCTG 2 cut(s) 926, 995
Ama87I CYCGRG 1 cut(s) 1934
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 361, 466
ApaLI GTGCAC 1 cut(s) 730
ApeKI GCWGC 4 cut(s) 139, 657, 989, 1374
ApoI RAATTY 7 cut(s) 253, 626, 689, 737, 1401, 1640, 1716
AseI ATTAAT 1 cut(s) 708
Asp700I GAANNNNTTC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 361, 1393, 2026
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 5 cut(s) 455, 756, 983, 1131, 1648
AsuII TTCGAA 1 cut(s) 1334
AvaI CYCGRG 1 cut(s) 1934
AvaII GGWCC 2 cut(s) 1393, 2026
AxyI CCTNAGG 1 cut(s) 2208
BaeGI GKGCMC 1 cut(s) 734
BamHI GGATCC 3 cut(s) 30, 1265, 2324
BanII GRGCYC 2 cut(s) 1062, 1124
BbsI GAAGAC 1 cut(s) 1773
Bbv12I GWGCWC 4 cut(s) 10, 734, 1062, 1124
BbvI GCAGC 4 cut(s) 126, 669, 1001, 1361
BceAI ACGGC 1 cut(s) 243
BciT130I CCWGG 3 cut(s) 1752, 2039, 2388
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 7 cut(s) 405, 467, 917, 1298, 1878, 1931, 2068
BfaI CTAG 6 cut(s) 1230, 1479, 1550, 1622, 1773, 2052
BfmI CTRYAG 1 cut(s) 1197
BfuAI ACCTGC 1 cut(s) 1679
BisI GCNGC 4 cut(s) 140, 658, 990, 1375
BlpI GCTNAGC 1 cut(s) 825
BlsI GCNGC 4 cut(s) 141, 659, 991, 1376
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 4 cut(s) 35, 1752, 2039, 2388
Bme18I GGWCC 2 cut(s) 1393, 2026
BmeT110I CYCGRG 1 cut(s) 1934
BmgT120I GGNCC 3 cut(s) 361, 1393, 2026
BmiI GGNNCC 5 cut(s) 32, 1267, 1395, 2028, 2326
BmrFI CCNGG 4 cut(s) 35, 1752, 2039, 2388
BmrI ACTGGG 1 cut(s) 378
BmsI GCATC 6 cut(s) 178, 478, 889, 943, 1302, 1685
BmuI ACTGGG 1 cut(s) 378
BoxI GACNNNNGTC 1 cut(s) 1172
BpiI GAAGAC 1 cut(s) 1773
BplI GAGNNNNNCTC 2 cut(s) 2048, 2080
BpmI CTGGAG 3 cut(s) 524, 1188, 2129
Bpu1102I GCTNAGC 1 cut(s) 825
Bpu14I TTCGAA 1 cut(s) 1334
BpuEI CTTGAG 2 cut(s) 491, 1138
BpuMI CCSGG 1 cut(s) 35
Bsa29I ATCGAT 1 cut(s) 897
BsaBI GATNNNNATC 1 cut(s) 840
BsaHI GRCGYC 3 cut(s) 476, 1662, 2179
BsaJI CCNNGG 4 cut(s) 225, 357, 1389, 2386
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 5 cut(s) 425, 1148, 1622, 1840, 1841
Bse118I RCCGGY 1 cut(s) 866
Bse1I ACTGG 4 cut(s) 173, 384, 830, 1205
Bse21I CCTNAGG 1 cut(s) 2208
Bse3DI GCAATG 6 cut(s) 327, 346, 1417, 2010, 2142, 2307
Bse8I GATNNNNATC 1 cut(s) 840
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 3 cut(s) 1752, 2039, 2388
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 4 cut(s) 225, 357, 1389, 2386
BseGI GGATG 7 cut(s) 46, 76, 493, 628, 634, 958, 2490
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 5 cut(s) 425, 1148, 1622, 1840, 1841
BseMI GCAATG 6 cut(s) 327, 346, 1417, 2010, 2142, 2307
BseMII CTCAG 5 cut(s) 606, 1173, 1586, 1806, 2199
BseNI ACTGG 4 cut(s) 173, 384, 830, 1205
BseRI GAGGAG 2 cut(s) 1070, 2107
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 4 cut(s) 126, 669, 1001, 1361
BsgI GTGCAG 2 cut(s) 123, 1360
Bsh1236I CGCG 1 cut(s) 2469
Bsh1285I CGRYCG 1 cut(s) 1173
BshFI GGCC 2 cut(s) 363, 468
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 1173
BsiHKAI GWGCWC 4 cut(s) 10, 734, 1062, 1124
BsiHKCI CYCGRG 1 cut(s) 1934
BsiSI CCGG 3 cut(s) 35, 279, 867
BslI CCNNNNNNNGG 5 cut(s) 425, 1148, 1622, 1840, 1841
BsmAI GTCTC 7 cut(s) 405, 467, 917, 1298, 1878, 1931, 2068
BsmBI CGTCTC 2 cut(s) 405, 467
BsmI GAATGC 3 cut(s) 1690, 2089, 2442
BsnI GGCC 2 cut(s) 363, 468
BsoBI CYCGRG 1 cut(s) 1934
Bsp119I TTCGAA 1 cut(s) 1334
Bsp1286I GDGCHC 4 cut(s) 10, 734, 1062, 1124
Bsp13I TCCGGA 1 cut(s) 278
Bsp1720I GCTNAGC 1 cut(s) 825
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 3 cut(s) 38, 1559, 2495
BspANI GGCC 2 cut(s) 363, 468
BspCNI CTCAG 5 cut(s) 607, 1174, 1585, 1807, 2200
BspDI ATCGAT 1 cut(s) 897
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 1 cut(s) 2469
BspHI TCATGA 2 cut(s) 886, 1068
BspLI GGNNCC 5 cut(s) 32, 1267, 1395, 2028, 2326
BspMI ACCTGC 1 cut(s) 1679
BspT104I TTCGAA 1 cut(s) 1334
BsrDI GCAATG 6 cut(s) 327, 346, 1417, 2010, 2142, 2307
BsrFI RCCGGY 1 cut(s) 866
BsrI ACTGG 4 cut(s) 173, 384, 830, 1205
BssAI RCCGGY 1 cut(s) 866
BssECI CCNNGG 4 cut(s) 225, 357, 1389, 2386
BssNI GRCGYC 3 cut(s) 476, 1662, 2179
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 3 cut(s) 1752, 2039, 2388
Bst4CI ACNGT 3 cut(s) 130, 779, 1026
Bst6I CTCTTC 1 cut(s) 2257
BstACI GRCGYC 3 cut(s) 476, 1662, 2179
BstBI TTCGAA 1 cut(s) 1334
BstC8I GCNNGC 2 cut(s) 93, 199
BstDSI CCRYGG 2 cut(s) 225, 357
BstEII GGTNACC 1 cut(s) 1137
BstENI CCTNNNNNAGG 1 cut(s) 1620
BstF5I GGATG 7 cut(s) 46, 76, 493, 628, 634, 958, 2490
BstFNI CGCG 1 cut(s) 2469
BstMAI GTCTC 7 cut(s) 405, 467, 917, 1298, 1878, 1931, 2068
BstMCI CGRYCG 1 cut(s) 1173
BstMWI GCNNNNNNNGC 3 cut(s) 101, 908, 1537
BstNI CCWGG 3 cut(s) 1752, 2039, 2388
BstNSI RCATGY 1 cut(s) 864
BstPAI GACNNNNGTC 1 cut(s) 1172
BstPI GGTNACC 1 cut(s) 1137
BstSCI CCNGG 4 cut(s) 33, 1750, 2037, 2386
BstSFI CTRYAG 1 cut(s) 1197
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 1 cut(s) 2469
BstV1I GCAGC 4 cut(s) 126, 669, 1001, 1361
BstV2I GAAGAC 1 cut(s) 1773
BstX2I RGATCY 3 cut(s) 30, 1265, 2324
BstXI CCANNNNNNTGG 1 cut(s) 1672
BstYI RGATCY 3 cut(s) 30, 1265, 2324
Bsu15I ATCGAT 1 cut(s) 897
Bsu36I CCTNAGG 1 cut(s) 2208
BsuRI GGCC 2 cut(s) 363, 468
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 2 cut(s) 225, 357
BtgZI GCGATG 2 cut(s) 885, 1983
BtsCI GGATG 7 cut(s) 46, 76, 493, 628, 634, 958, 2490
BtsI GCAGTG 2 cut(s) 111, 2120
BtsIMutI CAGTG 2 cut(s) 111, 2120
BveI ACCTGC 1 cut(s) 1679
Cac8I GCNNGC 2 cut(s) 93, 199
CaiI CAGNNNCTG 2 cut(s) 926, 995
CciI TCATGA 2 cut(s) 886, 1068
Cfr10I RCCGGY 1 cut(s) 866
Cfr13I GGNCC 3 cut(s) 361, 1393, 2026
ClaI ATCGAT 1 cut(s) 897
CseI GACGC 2 cut(s) 1670, 2168
Csp6I GTAC 6 cut(s) 1002, 1233, 1566, 1737, 1979, 2009
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviQI GTAC 6 cut(s) 1002, 1233, 1566, 1737, 1979, 2009
DraI TTTAAA 1 cut(s) 1041
Eam1104I CTCTTC 1 cut(s) 2257
EarI CTCTTC 1 cut(s) 2257
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 2 cut(s) 1060, 1122
Eco130I CCWWGG 1 cut(s) 357
Eco24I GRGCYC 2 cut(s) 1062, 1124
Eco32I GATATC 2 cut(s) 1420, 2286
Eco47I GGWCC 2 cut(s) 1393, 2026
Eco53kI GAGCTC 2 cut(s) 1060, 1122
Eco57I CTGAAG 6 cut(s) 204, 948, 1026, 1470, 1781, 1938
Eco81I CCTNAGG 1 cut(s) 2208
Eco88I CYCGRG 1 cut(s) 1934
Eco91I GGTNACC 1 cut(s) 1137
EcoICRI GAGCTC 2 cut(s) 1060, 1122
EcoNI CCTNNNNNAGG 1 cut(s) 1620
EcoO109I RGGNCCY 1 cut(s) 1393
EcoO65I GGTNACC 1 cut(s) 1137
EcoRI GAATTC 1 cut(s) 626
EcoRII CCWGG 3 cut(s) 1750, 2037, 2386
EcoRV GATATC 2 cut(s) 1420, 2286
EcoT14I CCWWGG 1 cut(s) 357
EcoT38I GRGCYC 2 cut(s) 1062, 1124
ErhI CCWWGG 1 cut(s) 357
Esp3I CGTCTC 2 cut(s) 405, 467
FauNDI CATATG 2 cut(s) 534, 567
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 140, 658, 990, 1375
FokI GGATG 7 cut(s) 53, 83, 500, 621, 635, 965, 2477
FriOI GRGCYC 2 cut(s) 1062, 1124
Fsp4HI GCNGC 4 cut(s) 140, 658, 990, 1375
FspBI CTAG 6 cut(s) 1230, 1479, 1550, 1622, 1773, 2052
GluI GCNGC 4 cut(s) 140, 658, 990, 1375
GsuI CTGGAG 3 cut(s) 524, 1188, 2129
HaeIII GGCC 2 cut(s) 363, 468
HapII CCGG 3 cut(s) 35, 279, 867
HgaI GACGC 2 cut(s) 1670, 2168
Hin1I GRCGYC 3 cut(s) 476, 1662, 2179
HindIII AAGCTT 1 cut(s) 1793
HinfI GANTC 9 cut(s) 86, 275, 611, 1214, 1222, 1306, 1755, 2097, 2237
HpaII CCGG 3 cut(s) 35, 279, 867
HphI GGTGA 5 cut(s) 455, 756, 983, 1131, 1648
Hpy166II GTNNAC 2 cut(s) 732, 1476
Hpy8I GTNNAC 2 cut(s) 732, 1476
Hpy99I CGWCG 1 cut(s) 481
HpyAV CCTTC 9 cut(s) 737, 1001, 1136, 1236, 1697, 1756, 1901, 2261, 2338
HpyCH4III ACNGT 3 cut(s) 130, 779, 1026
HpyCH4IV ACGT 1 cut(s) 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 908, 1537
HpySE526I ACGT 1 cut(s) 476
Hsp92I GRCGYC 3 cut(s) 476, 1662, 2179
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 1 cut(s) 700
LmnI GCTCC 9 cut(s) 19, 543, 997, 1057, 1127, 1207, 1858, 2110, 2156
Lsp1109I GCAGC 4 cut(s) 126, 669, 1001, 1361
LweI GCATC 6 cut(s) 178, 478, 889, 943, 1302, 1685
MaeI CTAG 6 cut(s) 1230, 1479, 1550, 1622, 1773, 2052
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 4 cut(s) 311, 578, 1137, 2276
MboII GAAGA 6 cut(s) 197, 602, 1295, 1314, 1778, 2274
MflI RGATCY 3 cut(s) 30, 1265, 2324
MhlI GDGCHC 4 cut(s) 10, 734, 1062, 1124
MluI ACGCGT 1 cut(s) 2467
MlyI GAGTC 3 cut(s) 95, 1208, 1300
MmeI TCCRAC 2 cut(s) 63, 459
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 300
MslI CAYNNNNRTG 4 cut(s) 405, 1067, 1670, 1698
MspI CCGG 3 cut(s) 35, 279, 867
MspR9I CCNGG 4 cut(s) 35, 1752, 2039, 2388
Mva1269I GAATGC 3 cut(s) 1690, 2089, 2442
MvaI CCWGG 3 cut(s) 1752, 2039, 2388
MvnI CGCG 1 cut(s) 2469
MwoI GCNNNNNNNGC 3 cut(s) 101, 908, 1537
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 534, 567
NlaIV GGNNCC 5 cut(s) 32, 1267, 1395, 2028, 2326
NmuCI GTSAC 1 cut(s) 1137
NspI RCATGY 1 cut(s) 864
NspV TTCGAA 1 cut(s) 1334
PagI TCATGA 2 cut(s) 886, 1068
PctI GAATGC 3 cut(s) 1690, 2089, 2442
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 6 cut(s) 275, 611, 1222, 1755, 2097, 2237
PflFI GACNNNGTC 1 cut(s) 2394
PkrI GCNGC 4 cut(s) 141, 659, 991, 1376
Ple19I CGATCG 1 cut(s) 1173
PleI GAGTC 3 cut(s) 94, 1208, 1300
PpsI GAGTC 3 cut(s) 94, 1208, 1300
PpuMI RGGWCCY 1 cut(s) 1393
PshAI GACNNNNGTC 1 cut(s) 1172
PshBI ATTAAT 1 cut(s) 708
Psp124BI GAGCTC 2 cut(s) 1062, 1124
Psp5II RGGWCCY 1 cut(s) 1393
Psp6I CCWGG 3 cut(s) 1750, 2037, 2386
PspEI GGTNACC 1 cut(s) 1137
PspGI CCWGG 3 cut(s) 1750, 2037, 2386
PspN4I GGNNCC 5 cut(s) 32, 1267, 1395, 2028, 2326
PspPI GGNCC 3 cut(s) 361, 1393, 2026
PspPPI RGGWCCY 1 cut(s) 1393
PstNI CAGNNNCTG 2 cut(s) 926, 995
PsuI RGATCY 3 cut(s) 30, 1265, 2324
PsyI GACNNNGTC 1 cut(s) 2394
PvuI CGATCG 1 cut(s) 1173
RsaI GTAC 6 cut(s) 1003, 1234, 1567, 1738, 1980, 2010
RsaNI GTAC 6 cut(s) 1002, 1233, 1566, 1737, 1979, 2009
RseI CAYNNNNRTG 4 cut(s) 405, 1067, 1670, 1698
SacI GAGCTC 2 cut(s) 1062, 1124
SatI GCNGC 4 cut(s) 140, 658, 990, 1375
Sau96I GGNCC 3 cut(s) 361, 1393, 2026
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 3 cut(s) 95, 1208, 1300
ScrFI CCNGG 4 cut(s) 35, 1752, 2039, 2388
SduI GDGCHC 4 cut(s) 10, 734, 1062, 1124
SfaNI GCATC 6 cut(s) 178, 478, 889, 943, 1302, 1685
SfcI CTRYAG 1 cut(s) 1197
SfuI TTCGAA 1 cut(s) 1334
SgrAI CRCCGGYG 1 cut(s) 866
SinI GGWCC 2 cut(s) 1393, 2026
SmiMI CAYNNNNRTG 4 cut(s) 405, 1067, 1670, 1698
SmlI CTYRAG 2 cut(s) 470, 1117
SmoI CTYRAG 2 cut(s) 470, 1117
SsiI CCGC 3 cut(s) 38, 1559, 2495
SspMI CTAG 6 cut(s) 1230, 1479, 1550, 1622, 1773, 2052
SstI GAGCTC 2 cut(s) 1062, 1124
StyD4I CCNGG 4 cut(s) 33, 1750, 2037, 2386
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 3 cut(s) 130, 779, 1026
TaiI ACGT 1 cut(s) 479
TaqI TCGA 7 cut(s) 834, 897, 1334, 1962, 2290, 2424, 2451
TatI WGTACW 3 cut(s) 1001, 1232, 1736
TfiI GAWTC 6 cut(s) 275, 611, 1222, 1755, 2097, 2237
TscAI CASTG 2 cut(s) 111, 2120
TseFI GTSAC 1 cut(s) 1137
TseI GCWGC 4 cut(s) 139, 657, 989, 1374
Tsp45I GTSAC 1 cut(s) 1137
TspGWI ACGGA 3 cut(s) 6, 2169, 2321
TspRI CASTG 2 cut(s) 111, 2120
Tth111I GACNNNGTC 1 cut(s) 2394
VneI GTGCAC 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 1393, 2026
VspI ATTAAT 1 cut(s) 708
XagI CCTNNNNNAGG 1 cut(s) 1620
XapI RAATTY 7 cut(s) 253, 626, 689, 737, 1401, 1640, 1716
XceI RCATGY 1 cut(s) 864
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 6 cut(s) 1230, 1479, 1550, 1622, 1773, 2052
ZraI GACGTC 1 cut(s) 477
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.