Rw6G036760

N-terminal region of micro-spherule protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0005427
Physical Location & Seq
Forward (+)
61174381 .. 61179725
5345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036760.1

Sequence Viewer

Length: 2391 bp
ATGGCCGGGGACGCAGCTGCGGTGCGGAGCTCGGCCTTCGGTGCTTGGATTCCGGCGGACGATGTTTTGCTCAAGAACGCTGTTGAGGCTGGTGCTTCCTTGGAATCACTTGCTAAAGGTGCGGTTCATTTTTCTCGAAGGTTTACTGTGCAAGAACTGCAAGATCGATGGTACTCTATCCTATACGATCCAGTTGTGGCTGAAGAGGCTTCTGCTCGCATGACTGAGTTTGAGTGTTCCGCTCCAAATTCCACATCAAAGTTTAGTAGGTTAGGAAGTTCAAAAGAAAACAAATGTGTTCCTGGAAAGAGAAAAGCTGAAAGTGTTCGGAGTAGTTACTATGCTCTGCGTAAAAGGATCTGCAGTGAGCCATTTGACCCCATGGACCTCAATTTTATACTTGCACCCAGTGATAGCAACTATATTGTAAATGGAGATGGGCCTCTATCTGAACATGTCATGACTGGAGATCCAATCCCAAATCATTTTGGAGTTGAGGGATCAGATATGGACAATACTATGCATCATATGTTTCCACAAAATCTGATGCATGACAGCACTGCTATTGGTGGTATTGTTACTGTGGATACATTTCAGATTGGAGGGTTCCACAAGCCTGCTGAAGAAGATTTTTTAGTTGAGCAAGATAATCTACGTGAAGAAGCTCCTTACATTGAAGACAATCTGCCTATCACAGGGAATGAATCTGAGGTCAAGGAATTTGGTCAACCAAAGGAATTGCCGGATTGCAGCATGTTCGGTGCTGGCGATCTAGGAATGGAACCTCCTTGTTCACTTGATCAAATTAATAATGACGAGGTAAATATGTGTTCCCCATTTGAAGGGAATCAGGTTTTTAATGTATCTGCTTCAGACAGTAGTGCATCATTTCACGACTTGGAGTATTCAACTCAACTTACTGGCACACATATCTGGAGCACAGTTCCAGCAACAACTACGCCAGTTGATGTTGGTATCAGAGAGAATGATACGTGTACCAGAGATTCTTTTGAACTTCTTGACGATATTGGTGCTAATAACACTACAATATCAGGGTATGATATTGACTTGAGGACGGAAGTGGCAGTCAGTGATTTCAAGAGTCCAGCTAGTACTGAAGTTTATTTGGCAGAATTGTCCAATTCCCTTTTGAACTTTACAAGTGAGGAGGAACTTATGTTCAAAGATGAGATCGATAAGTCTTACTATGATGGCCTGAGCTCACTTTTGATGAGTTCGCCAAAGGATGATGTTGAAGAACACATGATTAATCTAATTGAGCGAGAGACATTGATGGCTCCAGTTATGTACCCTATGAATCCCTCTGGTACAGATCCTGCAGTCACAGATGACATAACGGTGTCCCTCCCTGGTGATGATATGAATTGCCATCCAGAGACGCTGATGCAATCATTTCCAACAGCTTTGAACTCTCAGTTTCCCGAATATAAAGATGGAGTTATCTGTTGCACGTTAAATACTGAGGAATGGGAAATTCCATGCAACGATGACGTGTTTCTTCTCAATCACATGCCATTGTCATCAACTTCCTGTAAAGTAAAAGAAGCCAGTAAGCCAAAGTTGAAATGTTCATCTGTTAAGGATCTCGCAGTTAATCAAAGAAAGGGTGATATAGGCCCCTGCTTTATGCTAAAAGAACAAAGGAATCCTGGAAAATCTCTTGGATCTCAGAGTAAAGGATCACGCTGTATACCAGAAGTAAGTTCAAAATCACCTCTGTCTAATTTTGAGTTGTCCAAGACTGACCCCGTTGATGTGGCTTCTACGAGTACATGTCATGTCAGTGGTAATCGGGGTCAAATTGATTCAGCTAAAGCAATCACAAATCCTCTTCCTGGAATTATGAAGGAAGACACTAGAGATGGTACATTGACAATGTGTCTTGGGTATGCCTCAACAGAGTCTCATATGGCAAACTCATGTGTTGATTATGATTGTGTTAGAAGCTATCCTCAGCCATATGCTACTGAAACTAATTGGGAATCTGATGCATCCGCTACAATTAGAGATCATCAATCATTACATGCAGAAGTTGTGCCAATGGATAATGCTGTCTCAGAACCAGAAGTAAACCCAGTGACATCAGATTTTGAGGGTCTTTTCGATAGTGATGATGATATACCATGTTATTCGGATATAGAGGCAATGATACTTGACATGGATTTGGATCCAGATGACCAAGAAATGTATCATGGTGAGGAAGTCTCAAGATATCAGGATGACGACACTAGGAGAGCAATCATGAGACTGGAGCAGGGTGCTTATTCGTACATGCAAAGAGCCATTGCATCTCACGGAGCATTTGCCATTTTGTATGGGCGTCATTCTAAGCATTATATTAAGAAGCCTGAGGTTTGGAATTGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0002151 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0004857 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0005737 GO:0005783 GO:0005844 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010521 GO:0010556 GO:0010558 GO:0010605 GO:0010639 GO:0012505 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0030234 GO:0030425 GO:0031011 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032204 GO:0032205 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033044 GO:0033202 GO:0034046 GO:0034708 GO:0035097 GO:0036211 GO:0036477 GO:0042995 GO:0043005 GO:0043025 GO:0043086 GO:0043170 GO:0043204 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043981 GO:0043982 GO:0043984 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044297 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044454 GO:0044463 GO:0044464 GO:0044665 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051053 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051276 GO:0051338 GO:0051348 GO:0051972 GO:0051974 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070603 GO:0070717 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0097346 GO:0097447 GO:0097458 GO:0098772 GO:0120025 GO:0120038 GO:1900180 GO:1900182 GO:1901363 GO:1901564 GO:1902493 GO:1902494 GO:1903827 GO:1903829 GO:1904356 GO:1904357 GO:1904749 GO:1904751 GO:1904949 GO:1990234 GO:1990904 GO:2000112 GO:2000113 GO:2000278 GO:2000279 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
FHA MCRS_N

Protein Analysis

796

Amino Acids

87.84

Weight (kDa)

4.44

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MCRS_N PF13325 16 - 100 7.5e-15 N-terminal region of micro-spherule protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 1711
AciI CCGC 6 cut(s) 20, 25, 56, 122, 240, 2016
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 247, 719, 1494
AcuI CTGAAG 4 cut(s) 222, 642, 855, 1137
AcyI GRCGYC 1 cut(s) 2341
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 8 cut(s) 173, 997, 1114, 1310, 1330, 1792, 1888, 2291
AfiI CCNNNNNNNGG 3 cut(s) 695, 842, 1856
AflIII ACRYGT 4 cut(s) 454, 992, 1512, 1793
AhdI GACNNNNNGTC 1 cut(s) 1899
AjiI CACGTC 1 cut(s) 1513
AjnI CCWGG 4 cut(s) 301, 1369, 1669, 1855
AloI GAACNNNNNNTCC 2 cut(s) 1163, 1195
AluBI AGCT 9 cut(s) 17, 30, 317, 665, 1109, 1221, 1424, 1832, 1968
AluI AGCT 9 cut(s) 17, 30, 317, 665, 1109, 1221, 1424, 1832, 1968
Alw21I GWGCWC 3 cut(s) 32, 941, 1223
Alw26I GTCTC 6 cut(s) 1280, 1391, 1929, 2080, 2230, 2260
AlwNI CAGNNNCTG 1 cut(s) 1337
AoxI GGCC 5 cut(s) 3, 33, 440, 1213, 1636
ApeKI GCWGC 3 cut(s) 14, 17, 750
ApoI RAATTY 3 cut(s) 247, 719, 1494
AseI ATTAAT 2 cut(s) 807, 1269
Asp700I GAANNNNTTC 1 cut(s) 324
AspS9I GGNCC 3 cut(s) 385, 440, 1637
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 4 cut(s) 1385, 1640, 1725, 2228
AvaII GGWCC 1 cut(s) 385
AxyI CCTNAGG 1 cut(s) 2370
BamHI GGATCC 1 cut(s) 2188
BanII GRGCYC 2 cut(s) 32, 1223
BbsI GAAGAC 2 cut(s) 684, 1878
Bbv12I GWGCWC 3 cut(s) 32, 941, 1223
BbvCI CCTCAGC 1 cut(s) 1974
BbvI GCAGC 3 cut(s) 4, 26, 762
BccI CCATC 7 cut(s) 162, 431, 1205, 1288, 1398, 1448, 1877
BcgI CGANNNNNNTGC 4 cut(s) 739, 773, 1013, 1047
BciT130I CCWGG 4 cut(s) 303, 1371, 1671, 1857
BciVI GTATCC 1 cut(s) 580
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 6 cut(s) 1280, 1391, 1929, 2080, 2230, 2260
BfaI CTAG 4 cut(s) 773, 1110, 1878, 2250
BfmI CTRYAG 2 cut(s) 361, 1338
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 3 cut(s) 15, 18, 751
BlsI GCNGC 3 cut(s) 16, 19, 752
BmcAI AGTACT 1 cut(s) 1114
Bme1390I CCNGG 5 cut(s) 7, 303, 1371, 1671, 1857
Bme18I GGWCC 1 cut(s) 385
BmeRI GACNNNNNGTC 1 cut(s) 1899
BmgBI CACGTC 1 cut(s) 1513
BmgT120I GGNCC 3 cut(s) 385, 440, 1637
BmiI GGNNCC 5 cut(s) 608, 783, 1299, 1639, 2190
BmrFI CCNGG 5 cut(s) 7, 303, 1371, 1671, 1857
BmrI ACTGGG 2 cut(s) 402, 2090
BmsI GCATC 7 cut(s) 532, 537, 893, 1395, 1999, 2021, 2318
BmuI ACTGGG 2 cut(s) 402, 2090
BpiI GAAGAC 2 cut(s) 684, 1878
BplI GAGNNNNNCTC 2 cut(s) 2210, 2242
BpmI CTGGAG 4 cut(s) 486, 955, 1284, 2291
Bpu10I CCTNAGC 2 cut(s) 1217, 1974
BpuEI CTTGAG 3 cut(s) 56, 1090, 2212
BpuMI CCSGG 1 cut(s) 7
Bsa29I ATCGAT 2 cut(s) 166, 1194
BsaAI YACGTR 2 cut(s) 656, 993
BsaBI GATNNNNATC 1 cut(s) 2187
BsaHI GRCGYC 1 cut(s) 2341
BsaJI CCNNGG 4 cut(s) 6, 99, 381, 1369
Bsc4I CCNNNNNNNGG 3 cut(s) 695, 842, 1856
Bse1I ACTGG 9 cut(s) 191, 408, 469, 925, 962, 1301, 1569, 2096, 2274
Bse21I CCTNAGG 1 cut(s) 2370
Bse3DI GCAATG 2 cut(s) 2172, 2304
Bse8I GATNNNNATC 1 cut(s) 2187
BseBI CCWGG 4 cut(s) 303, 1371, 1671, 1857
BseCI ATCGAT 2 cut(s) 166, 1194
BseDI CCNNGG 4 cut(s) 6, 99, 381, 1369
BseGI GGATG 4 cut(s) 1252, 1390, 2012, 2245
BseJI GATNNNNATC 1 cut(s) 2187
BseLI CCNNNNNNNGG 3 cut(s) 695, 842, 1856
BseMI GCAATG 2 cut(s) 2172, 2304
BseMII CTCAG 9 cut(s) 216, 699, 1208, 1448, 1473, 1703, 1988, 2091, 2361
BseNI ACTGG 9 cut(s) 191, 408, 469, 925, 962, 1301, 1569, 2096, 2274
BseRI GAGGAG 1 cut(s) 1181
BseXI GCAGC 3 cut(s) 4, 26, 762
BshFI GGCC 5 cut(s) 5, 35, 442, 1215, 1638
BshVI ATCGAT 2 cut(s) 166, 1194
BsiHKAI GWGCWC 3 cut(s) 32, 941, 1223
BsiSI CCGG 3 cut(s) 6, 53, 743
BslFI GGGAC 2 cut(s) 23, 1348
BslI CCNNNNNNNGG 3 cut(s) 695, 842, 1856
BsmAI GTCTC 6 cut(s) 1280, 1391, 1929, 2080, 2230, 2260
BsmBI CGTCTC 1 cut(s) 1391
BsmFI GGGAC 2 cut(s) 23, 1348
BsnI GGCC 5 cut(s) 5, 35, 442, 1215, 1638
Bsp1286I GDGCHC 3 cut(s) 32, 941, 1223
Bsp19I CCATGG 1 cut(s) 381
BspACI CCGC 6 cut(s) 20, 25, 56, 122, 240, 2016
BspANI GGCC 5 cut(s) 5, 35, 442, 1215, 1638
BspCNI CTCAG 9 cut(s) 217, 700, 1209, 1447, 1474, 1702, 1987, 2090, 2362
BspDI ATCGAT 2 cut(s) 166, 1194
BspHI TCATGA 2 cut(s) 459, 2262
BspLI GGNNCC 5 cut(s) 608, 783, 1299, 1639, 2190
BspMAI CTGCAG 2 cut(s) 365, 1342
BsrBI CCGCTC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 2172, 2304
BsrI ACTGG 9 cut(s) 191, 408, 469, 925, 962, 1301, 1569, 2096, 2274
BssECI CCNNGG 4 cut(s) 6, 99, 381, 1369
BssNAI GTATAC 1 cut(s) 1712
BssNI GRCGYC 1 cut(s) 2341
BssT1I CCWWGG 2 cut(s) 99, 381
Bst1107I GTATAC 1 cut(s) 1712
Bst2UI CCWGG 4 cut(s) 303, 1371, 1671, 1857
Bst4CI ACNGT 5 cut(s) 148, 583, 878, 943, 1360
Bst6I CTCTTC 2 cut(s) 198, 1857
BstACI GRCGYC 1 cut(s) 2341
BstAPI GCANNNNNTGC 1 cut(s) 157
BstBAI YACGTR 2 cut(s) 656, 993
BstC8I GCNNGC 3 cut(s) 217, 618, 766
BstDSI CCRYGG 1 cut(s) 381
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 4 cut(s) 1252, 1390, 2012, 2245
BstMAI GTCTC 6 cut(s) 1280, 1391, 1929, 2080, 2230, 2260
BstMWI GCNNNNNNNGC 6 cut(s) 11, 41, 86, 119, 157, 206
BstNI CCWGG 4 cut(s) 303, 1371, 1671, 1857
BstNSI RCATGY 6 cut(s) 458, 757, 1534, 1797, 2048, 2296
BstSCI CCNGG 5 cut(s) 5, 301, 1369, 1669, 1855
BstSFI CTRYAG 2 cut(s) 361, 1338
BstV1I GCAGC 3 cut(s) 4, 26, 762
BstV2I GAAGAC 2 cut(s) 684, 1878
BstX2I RGATCY 6 cut(s) 357, 469, 1333, 1603, 1685, 2188
BstYI RGATCY 6 cut(s) 357, 469, 1333, 1603, 1685, 2188
BstZ17I GTATAC 1 cut(s) 1712
Bsu15I ATCGAT 2 cut(s) 166, 1194
Bsu36I CCTNAGG 1 cut(s) 2370
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 5 cut(s) 5, 35, 442, 1215, 1638
BsuTUI ATCGAT 2 cut(s) 166, 1194
BtgI CCRYGG 1 cut(s) 381
BtrI CACGTC 1 cut(s) 1513
BtsCI GGATG 4 cut(s) 1252, 1390, 2012, 2245
BtsI GCAGTG 2 cut(s) 370, 558
BtsIMutI CAGTG 6 cut(s) 370, 415, 558, 1096, 1810, 2103
Cac8I GCNNGC 3 cut(s) 217, 618, 766
CaiI CAGNNNCTG 1 cut(s) 1337
CciI TCATGA 2 cut(s) 459, 2262
Cfr13I GGNCC 3 cut(s) 385, 440, 1637
ClaI ATCGAT 2 cut(s) 166, 1194
CseI GACGC 3 cut(s) 20, 1408, 2330
Csp6I GTAC 8 cut(s) 172, 996, 1113, 1309, 1329, 1791, 1887, 2290
CviQI GTAC 8 cut(s) 172, 996, 1113, 1309, 1329, 1791, 1887, 2290
DraIII CACNNNGTG 1 cut(s) 410
DriI GACNNNNNGTC 1 cut(s) 1899
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 198, 1857
Eam1105I GACNNNNNGTC 1 cut(s) 1899
EarI CTCTTC 2 cut(s) 198, 1857
EciI GGCGGA 1 cut(s) 71
Ecl136II GAGCTC 2 cut(s) 30, 1221
Eco130I CCWWGG 2 cut(s) 99, 381
Eco24I GRGCYC 2 cut(s) 32, 1223
Eco32I GATATC 1 cut(s) 2234
Eco47I GGWCC 1 cut(s) 385
Eco53kI GAGCTC 2 cut(s) 30, 1221
Eco57I CTGAAG 4 cut(s) 222, 642, 855, 1137
Eco81I CCTNAGG 1 cut(s) 2370
EcoICRI GAGCTC 2 cut(s) 30, 1221
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoO109I RGGNCCY 1 cut(s) 1637
EcoRII CCWGG 4 cut(s) 301, 1369, 1669, 1855
EcoRV GATATC 1 cut(s) 2234
EcoT14I CCWWGG 2 cut(s) 99, 381
EcoT22I ATGCAT 3 cut(s) 525, 552, 2014
EcoT38I GRGCYC 2 cut(s) 32, 1223
ErhI CCWWGG 2 cut(s) 99, 381
Esp3I CGTCTC 1 cut(s) 1391
FaqI GGGAC 2 cut(s) 23, 1348
FauNDI CATATG 3 cut(s) 528, 1929, 1981
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 1 cut(s) 1711
Fnu4HI GCNGC 3 cut(s) 15, 18, 751
FokI GGATG 4 cut(s) 1259, 1377, 1999, 2252
FriOI GRGCYC 2 cut(s) 32, 1223
Fsp4HI GCNGC 3 cut(s) 15, 18, 751
FspBI CTAG 4 cut(s) 773, 1110, 1878, 2250
GluI GCNGC 3 cut(s) 15, 18, 751
GsuI CTGGAG 4 cut(s) 486, 955, 1284, 2291
HaeIII GGCC 5 cut(s) 5, 35, 442, 1215, 1638
HapII CCGG 3 cut(s) 6, 53, 743
HgaI GACGC 3 cut(s) 20, 1408, 2330
Hin1I GRCGYC 1 cut(s) 2341
HincII GTYRAC 1 cut(s) 728
HindII GTYRAC 1 cut(s) 728
HpaII CCGG 3 cut(s) 6, 53, 743
HphI GGTGA 4 cut(s) 1385, 1640, 1725, 2228
Hpy166II GTNNAC 6 cut(s) 144, 728, 794, 996, 1712, 2092
Hpy8I GTNNAC 6 cut(s) 144, 728, 794, 996, 1712, 2092
HpyAV CCTTC 4 cut(s) 46, 132, 836, 1861
HpyCH4III ACNGT 5 cut(s) 148, 583, 878, 943, 1360
HpyCH4IV ACGT 4 cut(s) 655, 992, 1472, 1512
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 41, 86, 119, 157, 206
HpySE526I ACGT 4 cut(s) 655, 992, 1472, 1512
Hsp92I GRCGYC 1 cut(s) 2341
Ksp22I TGATCA 1 cut(s) 799
LmnI GCTCC 7 cut(s) 27, 247, 670, 936, 1303, 2272, 2318
Lsp1109I GCAGC 3 cut(s) 4, 26, 762
LweI GCATC 7 cut(s) 532, 537, 893, 1395, 1999, 2021, 2318
MaeI CTAG 4 cut(s) 773, 1110, 1878, 2250
MaeII ACGT 4 cut(s) 655, 992, 1472, 1512
MaeIII GTNAC 4 cut(s) 335, 577, 1342, 2098
MbiI CCGCTC 1 cut(s) 242
MboII GAAGA 9 cut(s) 215, 635, 638, 671, 689, 1268, 1511, 1844, 1883
MflI RGATCY 6 cut(s) 357, 469, 1333, 1603, 1685, 2188
MhlI GDGCHC 3 cut(s) 32, 941, 1223
MlyI GAGTC 2 cut(s) 1111, 1931
MmeI TCCRAC 1 cut(s) 1442
Mph1103I ATGCAT 3 cut(s) 525, 552, 2014
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 8 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2389
MslI CAYNNNNRTG 2 cut(s) 1358, 1803
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 6, 53, 743
MspR9I CCNGG 5 cut(s) 7, 303, 1371, 1671, 1857
MvaI CCWGG 4 cut(s) 303, 1371, 1671, 1857
MwoI GCNNNNNNNGC 6 cut(s) 11, 41, 86, 119, 157, 206
NciI CCSGG 1 cut(s) 7
NcoI CCATGG 1 cut(s) 381
NdeI CATATG 3 cut(s) 528, 1929, 1981
NlaIV GGNNCC 5 cut(s) 608, 783, 1299, 1639, 2190
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 2 cut(s) 1342, 2098
NsiI ATGCAT 3 cut(s) 525, 552, 2014
NspI RCATGY 6 cut(s) 458, 757, 1534, 1797, 2048, 2296
PagI TCATGA 2 cut(s) 459, 2262
PciI ACATGT 2 cut(s) 454, 1793
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 9 cut(s) 49, 104, 704, 847, 1004, 1318, 1666, 1826, 2003
PfoI TCCNGGA 3 cut(s) 301, 1669, 1855
PkrI GCNGC 3 cut(s) 16, 19, 752
PleI GAGTC 2 cut(s) 1110, 1930
PpsI GAGTC 2 cut(s) 1110, 1930
Ppu21I YACGTR 2 cut(s) 656, 993
PscI ACATGT 2 cut(s) 454, 1793
PshBI ATTAAT 2 cut(s) 807, 1269
Psp124BI GAGCTC 2 cut(s) 32, 1223
Psp6I CCWGG 4 cut(s) 301, 1369, 1669, 1855
PspGI CCWGG 4 cut(s) 301, 1369, 1669, 1855
PspN4I GGNNCC 5 cut(s) 608, 783, 1299, 1639, 2190
PspPI GGNCC 3 cut(s) 385, 440, 1637
PstI CTGCAG 2 cut(s) 365, 1342
PstNI CAGNNNCTG 1 cut(s) 1337
PsuI RGATCY 6 cut(s) 357, 469, 1333, 1603, 1685, 2188
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 8 cut(s) 173, 997, 1114, 1310, 1330, 1792, 1888, 2291
RsaNI GTAC 8 cut(s) 172, 996, 1113, 1309, 1329, 1791, 1887, 2290
RseI CAYNNNNRTG 2 cut(s) 1358, 1803
SacI GAGCTC 2 cut(s) 32, 1223
SaqAI TTAA 8 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2389
SatI GCNGC 3 cut(s) 15, 18, 751
Sau96I GGNCC 3 cut(s) 385, 440, 1637
ScaI AGTACT 1 cut(s) 1114
SchI GAGTC 2 cut(s) 1111, 1931
ScrFI CCNGG 5 cut(s) 7, 303, 1371, 1671, 1857
SduI GDGCHC 3 cut(s) 32, 941, 1223
SfaNI GCATC 7 cut(s) 532, 537, 893, 1395, 1999, 2021, 2318
SfcI CTRYAG 2 cut(s) 361, 1338
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 2 cut(s) 1358, 1803
SmlI CTYRAG 3 cut(s) 71, 1069, 2227
SmoI CTYRAG 3 cut(s) 71, 1069, 2227
SsiI CCGC 6 cut(s) 20, 25, 56, 122, 240, 2016
SspMI CTAG 4 cut(s) 773, 1110, 1878, 2250
SstI GAGCTC 2 cut(s) 32, 1223
StyD4I CCNGG 5 cut(s) 5, 301, 1369, 1669, 1855
StyI CCWWGG 2 cut(s) 99, 381
TaaI ACNGT 5 cut(s) 148, 583, 878, 943, 1360
TaiI ACGT 4 cut(s) 658, 995, 1475, 1515
TaqI TCGA 4 cut(s) 136, 166, 1194, 2124
TatI WGTACW 2 cut(s) 1112, 1790
TfiI GAWTC 9 cut(s) 49, 104, 704, 847, 1004, 1318, 1666, 1826, 2003
Tru1I TTAA 8 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2389
Tru9I TTAA 8 cut(s) 807, 858, 1269, 1475, 1599, 1614, 2361, 2389
TscAI CASTG 6 cut(s) 370, 415, 565, 1096, 1810, 2103
TseFI GTSAC 2 cut(s) 1342, 2098
TseI GCWGC 3 cut(s) 14, 17, 750
Tsp45I GTSAC 2 cut(s) 1342, 2098
TspDTI ATGAA 6 cut(s) 116, 717, 1331, 1397, 1581, 1880
TspGWI ACGGA 2 cut(s) 1091, 2331
TspRI CASTG 6 cut(s) 370, 415, 565, 1096, 1810, 2103
VpaK11BI GGWCC 1 cut(s) 385
VspI ATTAAT 2 cut(s) 807, 1269
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 3 cut(s) 247, 719, 1494
XceI RCATGY 6 cut(s) 458, 757, 1534, 1797, 2048, 2296
XmiI GTMKAC 1 cut(s) 1711
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 4 cut(s) 773, 1110, 1878, 2250
ZrmI AGTACT 1 cut(s) 1114
Zsp2I ATGCAT 3 cut(s) 525, 552, 2014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.