AT1G76730

5-formyltetrahydrofolate cyclo-ligase-like protein COG0212

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009639
Physical Location & Seq
Reverse (-)
28802753 .. 28804858
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76730.1

Sequence Viewer

Length: 1065 bp
ATGATAAGATTATCACCTGGATTTGCAATCAATCCTCGTTTTCGATCCGATTCCTTTGTCAATTCCCAGAAACCGCCTTTTCTCTCCGTCCAAATCGGGTCACAGCGGAGGAATCTCTCTAGAATCGGATCGGAGAACGGCGACGGCGTCGCTTTCGATGCTGTAGCTTATGAGGCTGATCGGTTGAGCCTCGACGCGGCGGCGATGGAGGATATGGCGGAGACTGCAAAGAAGGAACTAGAATCGGATCCAGATAGTGACCCGAAAGCGTGGAAATGGGTTATCCGAAAGAAGATGTGGGATCTCATGGAAGCTCGGAACTACGCTATGAGCCCTAGACCTGTTCATCATCGAATCCCTAATTTCGTCGGTGCATCGGCTGCTGCAAGAAAATTAGCCGAGCTTGATGCTTTTCGTATGGCTATGGTCGTGAAGGTTAATCCCGATTCGCCTCAAAAGCAAATCAGGTTTCTTACACTTTCTGGTGAAAAGAAGCTGTTGACTCCTCAACCGAGGCTAAGAACAGGATTCTTCTCTGTACTAGAATCTGATTTGTTGAAACCCGAAACGATCATGGAGGCTTGCACTTCTGTAGGAGTAGCCAAATATGGAAGAGCGATTGGCCTTGATGAGAAAATCAAAGTGGATCTCATTGTCATTGGCTCTGTTGCGGTTAACCCACAAACGGGTGCTCGATTAGGGAAAGGAGAGGGTTTTGCGGAACTCGAATATGGAATGTTGCGTTACATGGGAGCCATTGATGATTCCACACCCGTTGTTACAACTGTACATGATTGTCAGCTTGTAGATGATATACCTCTCGAGAAACTGGCGATTCACGATGTTCCTGTGGATATCATATGCACTCCTACTCGAGTCATTTTCACAAACACACCAATACCAAAACCACAAGGAATTTATTGGGACAAACTGTCGCCGGAGAAGCTACGACAGATTCGAATACTAAGAGAGCTAAAGAATCGACTGGAAAAGAAGACTGGCCGTAAACTTCCGACTGGACCATCTGAGAAATTACCTCCTACGGCTGAGCGAAAACGCCGGTAA

Protein Analysis

354

Amino Acids

39.56

Weight (kDa)

9.41

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 92 - 290 3.5e-38 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 197
AciI CCGC 7 cut(s) 74, 106, 197, 200, 218, 671, 719
AclWI GGATC 6 cut(s) 39, 136, 242, 255, 309, 654
AcoI YGGCCR 1 cut(s) 1000
AcsI RAATTY 1 cut(s) 915
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 2 cut(s) 540, 789
AfiI CCNNNNNNNGG 3 cut(s) 196, 685, 686
AgsI TTSAA 1 cut(s) 559
AhdI GACNNNNNGTC 1 cut(s) 931
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 7 cut(s) 167, 314, 403, 496, 802, 946, 973
AluI AGCT 7 cut(s) 167, 314, 403, 496, 802, 946, 973
Alw21I GWGCWC 1 cut(s) 694
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 6 cut(s) 39, 136, 242, 255, 309, 654
Ama87I CYCGRG 2 cut(s) 821, 873
AoxI GGCC 2 cut(s) 622, 1000
ApeKI GCWGC 2 cut(s) 380, 383
ApoI RAATTY 1 cut(s) 915
AspS9I GGNCC 1 cut(s) 1019
AsuHPI GGTGA 2 cut(s) 6, 497
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 2 cut(s) 821, 873
AvaII GGWCC 1 cut(s) 1019
BamHI GGATCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 1001
Bbv12I GWGCWC 1 cut(s) 694
BbvI GCAGC 2 cut(s) 367, 370
BccI CCATC 2 cut(s) 199, 1030
BceAI ACGGC 4 cut(s) 154, 160, 987, 1059
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 18
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 4 cut(s) 120, 239, 336, 542
BfmI CTRYAG 2 cut(s) 162, 591
BisI GCNGC 4 cut(s) 198, 201, 381, 384
BlpI GCTNAGC 1 cut(s) 1047
BlsI GCNGC 4 cut(s) 199, 202, 382, 385
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 1019
BmeRI GACNNNNNGTC 1 cut(s) 931
BmeT110I CYCGRG 2 cut(s) 821, 873
BmgT120I GGNCC 1 cut(s) 1019
BmiI GGNNCC 2 cut(s) 249, 754
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 3 cut(s) 148, 383, 397
BpiI GAAGAC 1 cut(s) 1001
Bpu1102I GCTNAGC 1 cut(s) 1047
Bpu14I TTCGAA 1 cut(s) 958
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 512
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 685, 686
Bse118I RCCGGY 1 cut(s) 1059
Bse1I ACTGG 4 cut(s) 834, 990, 1003, 1021
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 512
BseLI CCNNNNNNNGG 3 cut(s) 196, 685, 686
BseMII CTCAG 2 cut(s) 1017, 1038
BseNI ACTGG 4 cut(s) 834, 990, 1003, 1021
BseRI GAGGAG 1 cut(s) 495
BseXI GCAGC 2 cut(s) 367, 370
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 2 cut(s) 624, 1002
BsiHKAI GWGCWC 1 cut(s) 694
BsiHKCI CYCGRG 2 cut(s) 821, 873
BsiSI CCGG 2 cut(s) 938, 1060
BslFI GGGAC 1 cut(s) 938
BslI CCNNNNNNNGG 3 cut(s) 196, 685, 686
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 938
BsnI GGCC 2 cut(s) 624, 1002
BsoBI CYCGRG 2 cut(s) 821, 873
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 2 cut(s) 335, 694
Bsp1407I TGTACA 1 cut(s) 787
Bsp143I GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
Bsp1720I GCTNAGC 1 cut(s) 1047
BspACI CCGC 7 cut(s) 74, 106, 197, 200, 218, 671, 719
BspANI GGCC 2 cut(s) 624, 1002
BspCNI CTCAG 2 cut(s) 1018, 1039
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 2 cut(s) 249, 754
BspPI GGATC 6 cut(s) 39, 136, 242, 255, 309, 654
BspQI GCTCTTC 1 cut(s) 607
BspT104I TTCGAA 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 1059
BsrGI TGTACA 1 cut(s) 787
BsrI ACTGG 4 cut(s) 834, 990, 1003, 1021
BssAI RCCGGY 1 cut(s) 1059
BssECI CCNNGG 1 cut(s) 512
BssMI GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
BssNI GRCGYC 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 787, 933
Bst6I CTCTTC 1 cut(s) 607
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 787
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 4 cut(s) 518, 965, 1026, 1047
BstFNI CGCG 1 cut(s) 197
BstKTI GATC 7 cut(s) 47, 131, 181, 250, 304, 573, 649
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
BstMWI GCNNNNNNNGC 6 cut(s) 158, 173, 224, 380, 457, 943
BstNI CCWGG 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 2 cut(s) 162, 591
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 367, 370
BstV2I GAAGAC 1 cut(s) 1001
BstX2I RGATCY 3 cut(s) 247, 301, 646
BstYI RGATCY 3 cut(s) 247, 301, 646
BsuRI GGCC 2 cut(s) 624, 1002
BtgZI GCGATG 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 583
Cfr10I RCCGGY 1 cut(s) 1059
Cfr13I GGNCC 1 cut(s) 1019
CseI GACGC 2 cut(s) 136, 203
Csp6I GTAC 2 cut(s) 539, 788
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 4 cut(s) 307, 574, 748, 791
CviQI GTAC 2 cut(s) 539, 788
DdeI CTNAG 4 cut(s) 518, 965, 1026, 1047
DpnI GATC 7 cut(s) 46, 130, 180, 249, 303, 572, 648
DpnII GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
DriI GACNNNNNGTC 1 cut(s) 931
EaeI YGGCCR 1 cut(s) 1000
Eam1104I CTCTTC 1 cut(s) 607
Eam1105I GACNNNNNGTC 1 cut(s) 931
EarI CTCTTC 1 cut(s) 607
EciI GGCGGA 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 335
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 1 cut(s) 1019
Eco88I CYCGRG 2 cut(s) 821, 873
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 856
EcoT38I GRGCYC 1 cut(s) 335
FaeI CATG 4 cut(s) 310, 577, 751, 794
FaqI GGGAC 1 cut(s) 938
FatI CATG 4 cut(s) 306, 573, 747, 790
FauNDI CATATG 1 cut(s) 860
Fnu4HI GCNGC 4 cut(s) 198, 201, 381, 384
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 4 cut(s) 198, 201, 381, 384
FspBI CTAG 4 cut(s) 120, 239, 336, 542
GluI GCNGC 4 cut(s) 198, 201, 381, 384
HaeIII GGCC 2 cut(s) 624, 1002
HapII CCGG 2 cut(s) 938, 1060
HgaI GACGC 2 cut(s) 136, 203
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 4 cut(s) 310, 577, 751, 794
HincII GTYRAC 2 cut(s) 501, 676
HindII GTYRAC 2 cut(s) 501, 676
HpaI GTTAAC 1 cut(s) 676
HpaII CCGG 2 cut(s) 938, 1060
HphI GGTGA 2 cut(s) 6, 497
Hpy166II GTNNAC 3 cut(s) 501, 676, 1007
Hpy188I TCNGA 9 cut(s) 49, 128, 133, 247, 287, 318, 550, 1014, 1027
Hpy188III TCNNGA 7 cut(s) 120, 251, 430, 443, 821, 823, 839
Hpy8I GTNNAC 3 cut(s) 501, 676, 1007
Hpy99I CGWCG 4 cut(s) 146, 152, 197, 371
HpyAV CCTTC 2 cut(s) 226, 427
HpyCH4III ACNGT 2 cut(s) 787, 933
HpyCH4V TGCA 6 cut(s) 26, 227, 374, 386, 585, 864
HpyF10VI GCNNNNNNNGC 6 cut(s) 158, 173, 224, 380, 457, 943
HpyF3I CTNAG 4 cut(s) 518, 965, 1026, 1047
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 4 cut(s) 310, 577, 751, 794
KspAI GTTAAC 1 cut(s) 676
Kzo9I GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
LguI GCTCTTC 1 cut(s) 607
LmnI GCTCC 1 cut(s) 752
Lsp1109I GCAGC 2 cut(s) 367, 370
LweI GCATC 3 cut(s) 148, 383, 397
MaeI CTAG 4 cut(s) 120, 239, 336, 542
MaeIII GTNAC 4 cut(s) 99, 257, 743, 778
MalI GATC 7 cut(s) 46, 130, 180, 249, 303, 572, 648
MboI GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
MboII GAAGA 4 cut(s) 304, 523, 624, 1006
MflI RGATCY 3 cut(s) 247, 301, 646
MhlI GDGCHC 2 cut(s) 335, 694
MluCI AATT 5 cut(s) 61, 361, 392, 915, 1031
MlyI GAGTC 2 cut(s) 496, 885
MmeI TCCRAC 1 cut(s) 1037
MseI TTAA 2 cut(s) 438, 675
MspA1I CMGCKG 1 cut(s) 106
MspI CCGG 2 cut(s) 938, 1060
MspR9I CCNGG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 6 cut(s) 158, 173, 224, 380, 457, 943
NdeI CATATG 1 cut(s) 860
NdeII GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
NlaIII CATG 4 cut(s) 310, 577, 751, 794
NlaIV GGNNCC 2 cut(s) 249, 754
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 2 cut(s) 99, 257
NspV TTCGAA 1 cut(s) 958
PaeR7I CTCGAG 2 cut(s) 821, 873
PciSI GCTCTTC 1 cut(s) 607
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 955
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 4 cut(s) 199, 202, 382, 385
PleI GAGTC 2 cut(s) 496, 884
PpsI GAGTC 2 cut(s) 496, 884
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 2 cut(s) 249, 754
PspPI GGNCC 1 cut(s) 1019
PspXI VCTCGAGB 1 cut(s) 873
PsuI RGATCY 3 cut(s) 247, 301, 646
PsyI GACNNNGTC 1 cut(s) 146
RsaI GTAC 2 cut(s) 540, 789
RsaNI GTAC 2 cut(s) 539, 788
SapI GCTCTTC 1 cut(s) 607
SaqAI TTAA 2 cut(s) 438, 675
SatI GCNGC 4 cut(s) 198, 201, 381, 384
Sau3AI GATC 7 cut(s) 44, 128, 178, 247, 301, 570, 646
Sau96I GGNCC 1 cut(s) 1019
SchI GAGTC 2 cut(s) 496, 885
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 2 cut(s) 335, 694
SfaNI GCATC 3 cut(s) 148, 383, 397
SfcI CTRYAG 2 cut(s) 162, 591
Sfr274I CTCGAG 2 cut(s) 821, 873
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 1 cut(s) 1019
SlaI CTCGAG 2 cut(s) 821, 873
SmlI CTYRAG 2 cut(s) 821, 873
SmoI CTYRAG 2 cut(s) 821, 873
Sse9I AATT 5 cut(s) 61, 361, 392, 915, 1031
SsiI CCGC 7 cut(s) 74, 106, 197, 200, 218, 671, 719
SspMI CTAG 4 cut(s) 120, 239, 336, 542
StyD4I CCNGG 1 cut(s) 16
TaaI ACNGT 2 cut(s) 787, 933
TasI AATT 5 cut(s) 61, 361, 392, 915, 1031
TatI WGTACW 2 cut(s) 538, 787
TauI GCSGC 2 cut(s) 200, 203
Tru1I TTAA 2 cut(s) 438, 675
Tru9I TTAA 2 cut(s) 438, 675
TseFI GTSAC 2 cut(s) 99, 257
TseI GCWGC 2 cut(s) 380, 383
Tsp45I GTSAC 2 cut(s) 99, 257
TspDTI ATGAA 1 cut(s) 335
TspGWI ACGGA 1 cut(s) 76
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 1019
XapI RAATTY 1 cut(s) 915
XbaI TCTAGA 1 cut(s) 119
XhoI CTCGAG 2 cut(s) 821, 873
XspI CTAG 4 cut(s) 120, 239, 336, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.