Rh6DG194700

5-formyltetrahydrofolate cyclo-ligase-like protein COG0212

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009639
Physical Location & Seq
Reverse (-)
34823411 .. 34828433
5023 bp
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UTR
Exon/CDS
Intron
Rh6DG194700.1

Sequence Viewer

Length: 816 bp
ATGGGACGGTGCTTTTGGTCGCAGGAGATCAACATTGTTTTTGGGGAGAGTGAAATAAGAGGAAATGAAGAAGAAGAATATGGGATTACAAGGAACCCCAGGCCTGTTCACCACCGCATCCCCAACTTCGTCGGCGCCTCCGCTGCTGCCGATAAATTGGGCGGGTTGGAAGCGTTCCGTGTCGGGAACTGTGTGAAAGTGAACCCGGATTCTCCTCAGAAGCAAGTCAGGTTTCTCACACTCTCTGATGGGAAGAAGCTGTTAACTCCTCAACCCCGTTTACGGACTGGGTTTTTCTCTGTGCTTGAATCTGAAATGATGACTCCTAGTACTTTGAAAGAGGCTTGCACCTCTGTTGGGGTGGCCAAACACGGGCGGCCTATTGGATTAGATGAGAAAATCAAGGTTGATCTTATTGTCATTGGCTCCGTTGCTGTTGATCCCAGGACAGGTGCTCGACTTGGCAAGGGTGAGGGATTTGCAGAGCTTGAATATGGGATGCTGCGATACATGGGAGCCATTGATGATTCAACACCAGTTGTCACTACTGTGCATGACAGTCAATTAGTAGATGATATTCCAATTGAGAAGCTGTTAGTCCATGATGTCATATTTACCAACACATCAATTCCAAAGCCTCAAGGGATTTACTGGGACAAATTGTCTCCGGAGAAGCTGGGGCAAATTAAAATACTCAGAGAGCTGAAGAGAAGGATTGAACAAGAGACTGGACAAAAATTGCCATGCGGTCCATCAGAGAAACTGCCTCCCACAGCGCGAAGAGGAGCTAACTTGGTAGGTGGTAAAAGGATATAA

Protein Analysis

271

Amino Acids

29.88

Weight (kDa)

8.82

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 27 - 203 5.5e-28 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 667
AciI CCGC 5 cut(s) 115, 141, 162, 376, 747
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 331
AfiI CCNNNNNNNGG 4 cut(s) 282, 357, 372, 449
AgsI TTSAA 5 cut(s) 308, 337, 491, 531, 719
AhdI GACNNNNNGTC 1 cut(s) 661
AjnI CCWGG 2 cut(s) 98, 443
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 6 cut(s) 259, 487, 592, 676, 703, 788
AluI AGCT 6 cut(s) 259, 487, 592, 676, 703, 788
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 669, 719
AlwI GGATC 1 cut(s) 434
Aor13HI TCCGGA 1 cut(s) 667
AoxI GGCC 3 cut(s) 101, 363, 377
ApeKI GCWGC 3 cut(s) 143, 146, 502
ArsI GACNNNNNNTTYG 1 cut(s) 29
AspLEI GCGC 2 cut(s) 137, 778
AspS9I GGNCC 1 cut(s) 749
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 101, 482
AvaII GGWCC 1 cut(s) 749
BalI TGGCCA 1 cut(s) 365
BanI GGYRCC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 3 cut(s) 130, 133, 489
BccI CCATC 2 cut(s) 242, 760
BciT130I CCWGG 2 cut(s) 100, 445
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 2 cut(s) 669, 719
BfaI CTAG 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 4 cut(s) 144, 147, 377, 503
BlsI GCNGC 4 cut(s) 145, 148, 378, 504
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 3 cut(s) 100, 206, 445
Bme18I GGWCC 1 cut(s) 749
BmeRI GACNNNNNGTC 1 cut(s) 661
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 4 cut(s) 95, 136, 427, 517
BmrFI CCNGG 3 cut(s) 100, 206, 445
BmrI ACTGGG 2 cut(s) 297, 661
BmsI GCATC 2 cut(s) 126, 489
BmuI ACTGGG 2 cut(s) 297, 661
BpuEI CTTGAG 1 cut(s) 624
BpuMI CCSGG 1 cut(s) 206
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 98, 443
BsaWI WCCGGW 1 cut(s) 667
Bsc4I CCNNNNNNNGG 4 cut(s) 282, 357, 372, 449
Bse1I ACTGG 4 cut(s) 292, 536, 656, 733
BseAI TCCGGA 1 cut(s) 667
BseBI CCWGG 2 cut(s) 100, 445
BseDI CCNNGG 2 cut(s) 98, 443
BseGI GGATG 2 cut(s) 117, 504
BseLI CCNNNNNNNGG 4 cut(s) 282, 357, 372, 449
BseMII CTCAG 2 cut(s) 230, 709
BseNI ACTGG 4 cut(s) 292, 536, 656, 733
BseRI GAGGAG 3 cut(s) 204, 258, 798
BseXI GCAGC 3 cut(s) 130, 133, 489
BseYI CCCAGC 1 cut(s) 676
Bsh1236I CGCG 1 cut(s) 778
BshFI GGCC 3 cut(s) 103, 365, 379
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 457
BsiSI CCGG 2 cut(s) 206, 668
BslFI GGGAC 2 cut(s) 18, 668
BslI CCNNNNNNNGG 4 cut(s) 282, 357, 372, 449
BsmAI GTCTC 2 cut(s) 669, 719
BsmFI GGGAC 2 cut(s) 18, 668
BsnI GGCC 3 cut(s) 103, 365, 379
Bsp1286I GDGCHC 1 cut(s) 457
Bsp13I TCCGGA 1 cut(s) 667
Bsp143I GATC 3 cut(s) 27, 409, 439
BspACI CCGC 5 cut(s) 115, 141, 162, 376, 747
BspANI GGCC 3 cut(s) 103, 365, 379
BspCNI CTCAG 2 cut(s) 229, 708
BspEI TCCGGA 1 cut(s) 667
BspFNI CGCG 1 cut(s) 778
BspLI GGNNCC 4 cut(s) 95, 136, 427, 517
BspPI GGATC 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 134
BsrI ACTGG 4 cut(s) 292, 536, 656, 733
BssECI CCNNGG 2 cut(s) 98, 443
BssMI GATC 3 cut(s) 27, 409, 439
BssNI GRCGYC 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 100, 445
Bst4CI ACNGT 4 cut(s) 9, 191, 550, 560
Bst6I CTCTTC 2 cut(s) 701, 775
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 216, 695
BstF5I GGATG 2 cut(s) 117, 504
BstFNI CGCG 1 cut(s) 778
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 2 cut(s) 137, 778
BstKTI GATC 3 cut(s) 30, 412, 442
BstMAI GTCTC 2 cut(s) 669, 719
BstMBI GATC 3 cut(s) 27, 409, 439
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 2 cut(s) 100, 445
BstSCI CCNGG 3 cut(s) 98, 204, 443
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 3 cut(s) 130, 133, 489
BsuRI GGCC 3 cut(s) 103, 365, 379
BtsCI GGATG 2 cut(s) 117, 504
Cac8I GCNNGC 1 cut(s) 346
CfoI GCGC 2 cut(s) 137, 778
Cfr13I GGNCC 1 cut(s) 749
Csp6I GTAC 1 cut(s) 330
CviAII CATG 4 cut(s) 511, 554, 602, 744
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 216, 695
DinI GGCGCC 1 cut(s) 136
DpnI GATC 3 cut(s) 29, 411, 441
DpnII GATC 3 cut(s) 27, 409, 439
DriI GACNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 2 cut(s) 701, 775
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 2 cut(s) 701, 775
Eco147I AGGCCT 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 98, 443
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
FaeI CATG 4 cut(s) 514, 557, 605, 747
FaiI YATR 8 cut(s) 81, 495, 512, 555, 603, 611, 745, 814
FaqI GGGAC 2 cut(s) 18, 668
FatI CATG 4 cut(s) 510, 553, 601, 743
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 4 cut(s) 144, 147, 377, 503
FokI GGATG 2 cut(s) 104, 511
Fsp4HI GCNGC 4 cut(s) 144, 147, 377, 503
FspBI CTAG 1 cut(s) 327
GlaI GCGC 2 cut(s) 136, 777
GluI GCNGC 4 cut(s) 144, 147, 377, 503
GsaI CCCAGC 1 cut(s) 680
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 3 cut(s) 103, 365, 379
HapII CCGG 2 cut(s) 206, 668
HhaI GCGC 2 cut(s) 137, 778
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 4 cut(s) 514, 557, 605, 747
Hin6I GCGC 2 cut(s) 135, 776
HinP1I GCGC 2 cut(s) 135, 776
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 4 cut(s) 209, 308, 322, 527
HpaI GTTAAC 1 cut(s) 264
HpaII CCGG 2 cut(s) 206, 668
HphI GGTGA 2 cut(s) 101, 482
Hpy166II GTNNAC 4 cut(s) 109, 202, 264, 281
Hpy188I TCNGA 5 cut(s) 219, 247, 313, 698, 757
Hpy188III TCNNGA 2 cut(s) 184, 668
Hpy8I GTNNAC 4 cut(s) 109, 202, 264, 281
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 705
HpyCH4III ACNGT 4 cut(s) 9, 191, 550, 560
HpyCH4V TGCA 3 cut(s) 348, 482, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 2 cut(s) 216, 695
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 4 cut(s) 514, 557, 605, 747
HspAI GCGC 2 cut(s) 135, 776
KasI GGCGCC 1 cut(s) 134
Kpn2I TCCGGA 1 cut(s) 667
KspAI GTTAAC 1 cut(s) 264
Kzo9I GATC 3 cut(s) 27, 409, 439
LmnI GCTCC 3 cut(s) 431, 515, 785
Lsp1109I GCAGC 3 cut(s) 130, 133, 489
LweI GCATC 2 cut(s) 126, 489
MaeI CTAG 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 541
MalI GATC 3 cut(s) 29, 411, 441
MboI GATC 3 cut(s) 27, 409, 439
MboII GAAGA 6 cut(s) 80, 83, 86, 265, 718, 792
MfeI CAATTG 1 cut(s) 582
MhlI GDGCHC 1 cut(s) 457
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 7 cut(s) 155, 563, 582, 627, 659, 684, 737
MluNI TGGCCA 1 cut(s) 365
Mly113I GGCGCC 1 cut(s) 135
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 147
Mox20I TGGCCA 1 cut(s) 365
MroI TCCGGA 1 cut(s) 667
MscI TGGCCA 1 cut(s) 365
MseI TTAA 2 cut(s) 263, 687
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 2 cut(s) 206, 668
MspR9I CCNGG 3 cut(s) 100, 206, 445
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 2 cut(s) 100, 445
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 1 cut(s) 143
NarI GGCGCC 1 cut(s) 135
NciI CCSGG 1 cut(s) 206
NdeII GATC 3 cut(s) 27, 409, 439
NlaIII CATG 4 cut(s) 514, 557, 605, 747
NlaIV GGNNCC 4 cut(s) 95, 136, 427, 517
NmuCI GTSAC 1 cut(s) 541
OliI CACNNNNGTG 1 cut(s) 548
PceI AGGCCT 1 cut(s) 103
PfeI GAWTC 3 cut(s) 209, 308, 527
PkrI GCNGC 4 cut(s) 145, 148, 378, 504
PleI GAGTC 1 cut(s) 316
PluTI GGCGCC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 98, 443
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 2 cut(s) 98, 443
PspN4I GGNNCC 4 cut(s) 95, 136, 427, 517
PspPI GGNCC 1 cut(s) 749
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 2 cut(s) 263, 687
SatI GCNGC 4 cut(s) 144, 147, 377, 503
Sau3AI GATC 3 cut(s) 27, 409, 439
Sau96I GGNCC 1 cut(s) 749
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 3 cut(s) 100, 206, 445
SduI GDGCHC 1 cut(s) 457
SfaNI GCATC 2 cut(s) 126, 489
SfoI GGCGCC 1 cut(s) 136
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 639
SmoI CTYRAG 1 cut(s) 639
Sse9I AATT 7 cut(s) 155, 563, 582, 627, 659, 684, 737
SseBI AGGCCT 1 cut(s) 103
SsiI CCGC 5 cut(s) 115, 141, 162, 376, 747
SspDI GGCGCC 1 cut(s) 134
SspMI CTAG 1 cut(s) 327
StuI AGGCCT 1 cut(s) 103
StyD4I CCNGG 3 cut(s) 98, 204, 443
TaaI ACNGT 4 cut(s) 9, 191, 550, 560
TaqI TCGA 1 cut(s) 457
TasI AATT 7 cut(s) 155, 563, 582, 627, 659, 684, 737
TatI WGTACW 1 cut(s) 329
TauI GCSGC 1 cut(s) 379
TfiI GAWTC 3 cut(s) 209, 308, 527
Tru1I TTAA 2 cut(s) 263, 687
Tru9I TTAA 2 cut(s) 263, 687
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 3 cut(s) 143, 146, 502
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 1 cut(s) 81
TspGWI ACGGA 3 cut(s) 167, 298, 418
VpaK11BI GGWCC 1 cut(s) 749
XspI CTAG 1 cut(s) 327
ZrmI AGTACT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.