FvH4_2g13270

5-formyltetrahydrofolate cyclo-ligase-like protein COG0212

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009639
Physical Location & Seq
Reverse (-)
11591906 .. 11595434
3529 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13270.t5

Sequence Viewer

Length: 1011 bp
ATGTCAATGGATACAACTCGTCTTCTCCGGAATTCCCACCCCTTCACATTCCCACTCAGACTCTCTCCACCCCTCACCAAAACCCGCCATTATCTTCCCGCCACAAATTCCCGCAAACTCTTGTTCATGCTCAAGACAACCCGAAACGACGCCGTCCCCTTCGACGACGCCGCTTACGAGGCCGAGCGCCTAACCCTCGACGCCAAGGCTCGCCAAACCATGGCCGAGGAGGCCTCCAACGTCACGGAAGACCCGAAAGCTTGGAAATGGGTGATCCGGAAGAGAATCTGGGACATGATGGAGGCCCACAACGTAGCCCAGAACCCAAGGCCTGTCCACCACCGCATCCCCAACTTCGTCGGCGCCGCCGCCGCCGCCGATAAATTGGGGCGGTTGGAAGCTTTCCGTGTCGGGAATTGTGTGAAAGTTAACCCGGATTCTCCTCAGAAACAAGTCAGGTTTCTCACACTGTCTGATGGGAAGAAGTTGTTAACTCCTCAACCCCGTTTACGGACCGGGTTTTTCTCTGTGCTTGAGTCTCAGATGTTGACAGCTAGTACTATGAAGGAGGCGTGCACGTCTGTTGGAGTGGCAAAATACGGGCGGCCTATTGGATTAGATGAGAAGATCAAGGTTGATCTTATTGTCATCGGCTCCGTTGCTGTTGATCCCAGGACAGGTGCTCGACTTGGCAAGGGTGAGGGATTTGCGGAGCTTGAATATGGGATGCTGCGATACATGGGAGCTATTGATGATTCAACACCAGTTGTCACCACTGTGCATGACAGTCAATTAGTAGATGACATTCCAATTGAGAAGCTGTTAGTCCATGATGTACCAGTTGACATCATATGCACTCCAACCCAGGTCATTTTTACCAACACATCAATTCCAAAGCCTCAAGGTTGGTTAACTTCTGCAATCTGGTTTTGGTTTTTTCTTTTTCTTTTTTCTTGCAAAAAAAGAGAGAAAAGAAACCCCTTTAAAGAGTTAATTGAGTACAGACCCTAA

Protein Analysis

337

Amino Acids

38.01

Weight (kDa)

9.42

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 89 - 261 4e-27 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 220
AccIII TCCGGA 2 cut(s) 27, 276
AclWI GGATC 2 cut(s) 268, 662
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 2 cut(s) 31, 106
AcyI GRCGYC 4 cut(s) 150, 168, 201, 363
AfaI GTAC 3 cut(s) 559, 837, 1001
AfiI CCNNNNNNNGG 3 cut(s) 220, 510, 677
AgsI TTSAA 2 cut(s) 719, 759
AjiI CACGTC 1 cut(s) 579
AjnI CCWGG 2 cut(s) 671, 864
AleI CACNNNNGTG 1 cut(s) 776
AluBI AGCT 6 cut(s) 260, 401, 554, 715, 746, 820
AluI AGCT 6 cut(s) 260, 401, 554, 715, 746, 820
Alw21I GWGCWC 2 cut(s) 578, 685
Alw26I GTCTC 1 cut(s) 543
Alw44I GTGCAC 1 cut(s) 574
AlwI GGATC 2 cut(s) 268, 662
Aor13HI TCCGGA 2 cut(s) 27, 276
AoxI GGCC 6 cut(s) 180, 222, 231, 303, 329, 605
ApaLI GTGCAC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 730
ApoI RAATTY 2 cut(s) 31, 106
AspLEI GCGC 2 cut(s) 189, 365
AspS9I GGNCC 2 cut(s) 304, 513
AsuC2I CCSGG 2 cut(s) 434, 517
AsuHPI GGTGA 4 cut(s) 67, 283, 710, 763
AvaII GGWCC 1 cut(s) 513
BaeGI GKGCMC 1 cut(s) 578
BanI GGYRCC 1 cut(s) 362
BbsI GAAGAC 2 cut(s) 14, 255
Bbv12I GWGCWC 2 cut(s) 578, 685
BbvI GCAGC 1 cut(s) 717
BccI CCATC 2 cut(s) 292, 470
BceAI ACGGC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 673, 866
BciVI GTATCC 1 cut(s) 4
BcnI CCSGG 2 cut(s) 434, 517
BcoDI GTCTC 1 cut(s) 543
BfaI CTAG 1 cut(s) 555
BfoI RGCGCY 2 cut(s) 190, 366
BfuI GTATCC 1 cut(s) 4
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 7 cut(s) 171, 366, 369, 372, 375, 605, 731
BlsI GCNGC 7 cut(s) 172, 367, 370, 373, 376, 606, 732
BmcAI AGTACT 1 cut(s) 559
Bme1390I CCNGG 4 cut(s) 434, 517, 673, 866
Bme18I GGWCC 1 cut(s) 513
BmgBI CACGTC 1 cut(s) 579
BmgT120I GGNCC 2 cut(s) 304, 513
BmiI GGNNCC 2 cut(s) 364, 655
BmrFI CCNGG 4 cut(s) 434, 517, 673, 866
BmsI GCATC 2 cut(s) 354, 717
BpiI GAAGAC 2 cut(s) 14, 255
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuEI CTTGAG 3 cut(s) 116, 554, 885
BpuMI CCSGG 2 cut(s) 434, 517
BsaHI GRCGYC 4 cut(s) 150, 168, 201, 363
BsaJI CCNNGG 6 cut(s) 204, 219, 225, 326, 671, 864
BsaWI WCCGGW 2 cut(s) 27, 276
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 510, 677
Bse1I ACTGG 2 cut(s) 764, 839
BseAI TCCGGA 2 cut(s) 27, 276
BseBI CCWGG 2 cut(s) 673, 866
BseDI CCNNGG 6 cut(s) 204, 219, 225, 326, 671, 864
BseGI GGATG 2 cut(s) 345, 732
BseLI CCNNNNNNNGG 3 cut(s) 220, 510, 677
BseMII CTCAG 3 cut(s) 70, 458, 554
BseNI ACTGG 2 cut(s) 764, 839
BseRI GAGGAG 3 cut(s) 242, 432, 486
BseSI GKGCMC 1 cut(s) 578
BseXI GCAGC 1 cut(s) 717
BshFI GGCC 6 cut(s) 182, 224, 233, 305, 331, 607
BshNI GGYRCC 1 cut(s) 362
BsiHKAI GWGCWC 2 cut(s) 578, 685
BsiSI CCGG 4 cut(s) 28, 277, 434, 516
BslFI GGGAC 2 cut(s) 140, 305
BslI CCNNNNNNNGG 3 cut(s) 220, 510, 677
BsmAI GTCTC 1 cut(s) 543
BsmFI GGGAC 2 cut(s) 140, 305
BsnI GGCC 6 cut(s) 182, 224, 233, 305, 331, 607
Bsp1286I GDGCHC 2 cut(s) 578, 685
Bsp13I TCCGGA 2 cut(s) 27, 276
Bsp143I GATC 4 cut(s) 273, 627, 637, 667
Bsp19I CCATGG 1 cut(s) 219
BspANI GGCC 6 cut(s) 182, 224, 233, 305, 331, 607
BspCNI CTCAG 3 cut(s) 69, 457, 553
BspEI TCCGGA 2 cut(s) 27, 276
BspLI GGNNCC 2 cut(s) 364, 655
BspPI GGATC 2 cut(s) 268, 662
BspT107I GGYRCC 1 cut(s) 362
BsrI ACTGG 2 cut(s) 764, 839
BssECI CCNNGG 6 cut(s) 204, 219, 225, 326, 671, 864
BssMI GATC 4 cut(s) 273, 627, 637, 667
BssNI GRCGYC 4 cut(s) 150, 168, 201, 363
BssT1I CCWWGG 3 cut(s) 204, 219, 326
Bst2UI CCWGG 2 cut(s) 673, 866
Bst4CI ACNGT 3 cut(s) 471, 778, 788
Bst6I CTCTTC 1 cut(s) 275
BstACI GRCGYC 4 cut(s) 150, 168, 201, 363
BstC8I GCNNGC 2 cut(s) 211, 574
BstDEI CTNAG 3 cut(s) 56, 444, 540
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 345, 732
BstH2I RGCGCY 2 cut(s) 190, 366
BstHHI GCGC 2 cut(s) 189, 365
BstKTI GATC 4 cut(s) 276, 630, 640, 670
BstMAI GTCTC 1 cut(s) 543
BstMBI GATC 4 cut(s) 273, 627, 637, 667
BstMWI GCNNNNNNNGC 4 cut(s) 179, 230, 371, 374
BstNI CCWGG 2 cut(s) 673, 866
BstSCI CCNGG 4 cut(s) 432, 515, 671, 864
BstSLI GKGCMC 1 cut(s) 578
BstV1I GCAGC 1 cut(s) 717
BstV2I GAAGAC 2 cut(s) 14, 255
BsuI GTATCC 1 cut(s) 4
BsuRI GGCC 6 cut(s) 182, 224, 233, 305, 331, 607
BtgI CCRYGG 1 cut(s) 219
BtrI CACGTC 1 cut(s) 579
BtsCI GGATG 2 cut(s) 345, 732
BtsIMutI CAGTG 2 cut(s) 467, 774
Cac8I GCNNGC 2 cut(s) 211, 574
CfoI GCGC 2 cut(s) 189, 365
Cfr13I GGNCC 2 cut(s) 304, 513
CpoI CGGWCCG 1 cut(s) 513
CseI GACGC 3 cut(s) 158, 176, 209
Csp6I GTAC 3 cut(s) 558, 836, 1000
CspI CGGWCCG 1 cut(s) 513
CviAII CATG 6 cut(s) 127, 220, 295, 739, 782, 830
CviQI GTAC 3 cut(s) 558, 836, 1000
DdeI CTNAG 3 cut(s) 56, 444, 540
DinI GGCGCC 1 cut(s) 364
DpnI GATC 4 cut(s) 275, 629, 639, 669
DpnII GATC 4 cut(s) 273, 627, 637, 667
DraI TTTAAA 1 cut(s) 985
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Eco130I CCWWGG 3 cut(s) 204, 219, 326
Eco147I AGGCCT 2 cut(s) 233, 331
Eco47I GGWCC 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 31
EcoRII CCWGG 2 cut(s) 671, 864
EcoT14I CCWWGG 3 cut(s) 204, 219, 326
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 3 cut(s) 204, 219, 326
FaeI CATG 6 cut(s) 130, 223, 298, 742, 785, 833
FaqI GGGAC 2 cut(s) 140, 305
FatI CATG 6 cut(s) 126, 219, 294, 738, 781, 829
FauI CCCGC 3 cut(s) 92, 106, 119
FauNDI CATATG 1 cut(s) 851
Fnu4HI GCNGC 7 cut(s) 171, 366, 369, 372, 375, 605, 731
FokI GGATG 2 cut(s) 332, 739
Fsp4HI GCNGC 7 cut(s) 171, 366, 369, 372, 375, 605, 731
FspBI CTAG 1 cut(s) 555
GlaI GCGC 2 cut(s) 188, 364
GluI GCNGC 7 cut(s) 171, 366, 369, 372, 375, 605, 731
HaeII RGCGCY 2 cut(s) 190, 366
HaeIII GGCC 6 cut(s) 182, 224, 233, 305, 331, 607
HapII CCGG 4 cut(s) 28, 277, 434, 516
HgaI GACGC 3 cut(s) 158, 176, 209
HhaI GCGC 2 cut(s) 189, 365
Hin1I GRCGYC 4 cut(s) 150, 168, 201, 363
Hin1II CATG 6 cut(s) 130, 223, 298, 742, 785, 833
Hin6I GCGC 2 cut(s) 187, 363
HinP1I GCGC 2 cut(s) 187, 363
HincII GTYRAC 5 cut(s) 430, 492, 549, 844, 912
HindII GTYRAC 5 cut(s) 430, 492, 549, 844, 912
HindIII AAGCTT 2 cut(s) 258, 399
HinfI GANTC 5 cut(s) 60, 285, 437, 536, 755
HpaI GTTAAC 3 cut(s) 430, 492, 912
HpaII CCGG 4 cut(s) 28, 277, 434, 516
HphI GGTGA 4 cut(s) 67, 283, 710, 763
Hpy166II GTNNAC 8 cut(s) 337, 430, 492, 509, 549, 576, 844, 912
Hpy188I TCNGA 4 cut(s) 59, 447, 475, 543
Hpy188III TCNNGA 4 cut(s) 28, 133, 277, 412
Hpy8I GTNNAC 8 cut(s) 337, 430, 492, 509, 549, 576, 844, 912
Hpy99I CGWCG 5 cut(s) 152, 167, 170, 203, 362
HpyAV CCTTC 3 cut(s) 52, 169, 559
HpyCH4III ACNGT 3 cut(s) 471, 778, 788
HpyCH4IV ACGT 3 cut(s) 240, 312, 578
HpyCH4V TGCA 5 cut(s) 576, 781, 855, 920, 957
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 230, 371, 374
HpyF3I CTNAG 3 cut(s) 56, 444, 540
HpySE526I ACGT 3 cut(s) 240, 312, 578
Hsp92I GRCGYC 4 cut(s) 150, 168, 201, 363
Hsp92II CATG 6 cut(s) 130, 223, 298, 742, 785, 833
HspAI GCGC 2 cut(s) 187, 363
KasI GGCGCC 1 cut(s) 362
Kpn2I TCCGGA 2 cut(s) 27, 276
KspAI GTTAAC 3 cut(s) 430, 492, 912
Kzo9I GATC 4 cut(s) 273, 627, 637, 667
LmnI GCTCC 3 cut(s) 659, 712, 743
Lsp1109I GCAGC 1 cut(s) 717
LweI GCATC 2 cut(s) 354, 717
MaeI CTAG 1 cut(s) 555
MaeII ACGT 3 cut(s) 240, 312, 578
MaeIII GTNAC 2 cut(s) 241, 769
MalI GATC 4 cut(s) 275, 629, 639, 669
MboI GATC 4 cut(s) 273, 627, 637, 667
MboII GAAGA 6 cut(s) 14, 86, 260, 292, 493, 637
MfeI CAATTG 1 cut(s) 810
MhlI GDGCHC 2 cut(s) 578, 685
MluCI AATT 8 cut(s) 31, 106, 383, 415, 791, 810, 888, 993
Mly113I GGCGCC 1 cut(s) 363
MlyI GAGTC 2 cut(s) 54, 545
MmeI TCCRAC 4 cut(s) 261, 375, 565, 884
MroI TCCGGA 2 cut(s) 27, 276
MseI TTAA 5 cut(s) 429, 491, 911, 984, 992
MslI CAYNNNNRTG 1 cut(s) 776
MspI CCGG 4 cut(s) 28, 277, 434, 516
MspR9I CCNGG 4 cut(s) 434, 517, 673, 866
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 2 cut(s) 673, 866
MwoI GCNNNNNNNGC 4 cut(s) 179, 230, 371, 374
NarI GGCGCC 1 cut(s) 363
NciI CCSGG 2 cut(s) 434, 517
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 851
NdeII GATC 4 cut(s) 273, 627, 637, 667
NlaIII CATG 6 cut(s) 130, 223, 298, 742, 785, 833
NlaIV GGNNCC 2 cut(s) 364, 655
NmeAIII GCCGAG 2 cut(s) 208, 250
NmuCI GTSAC 2 cut(s) 241, 769
OliI CACNNNNGTG 1 cut(s) 776
PceI AGGCCT 2 cut(s) 233, 331
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 251
PfeI GAWTC 3 cut(s) 285, 437, 755
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 7 cut(s) 172, 367, 370, 373, 376, 606, 732
PleI GAGTC 2 cut(s) 54, 544
PluTI GGCGCC 1 cut(s) 366
PpsI GAGTC 2 cut(s) 54, 544
Psp6I CCWGG 2 cut(s) 671, 864
PspGI CCWGG 2 cut(s) 671, 864
PspN4I GGNNCC 2 cut(s) 364, 655
PspPI GGNCC 2 cut(s) 304, 513
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 3 cut(s) 559, 837, 1001
RsaNI GTAC 3 cut(s) 558, 836, 1000
RseI CAYNNNNRTG 1 cut(s) 776
Rsr2I CGGWCCG 1 cut(s) 513
RsrII CGGWCCG 1 cut(s) 513
SaqAI TTAA 5 cut(s) 429, 491, 911, 984, 992
SatI GCNGC 7 cut(s) 171, 366, 369, 372, 375, 605, 731
Sau3AI GATC 4 cut(s) 273, 627, 637, 667
Sau96I GGNCC 2 cut(s) 304, 513
ScaI AGTACT 1 cut(s) 559
SchI GAGTC 2 cut(s) 54, 545
ScrFI CCNGG 4 cut(s) 434, 517, 673, 866
SduI GDGCHC 2 cut(s) 578, 685
SfaNI GCATC 2 cut(s) 354, 717
SfiI GGCCNNNNNGGCC 1 cut(s) 230
SfoI GGCGCC 1 cut(s) 364
SinI GGWCC 1 cut(s) 513
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 3 cut(s) 131, 533, 900
SmoI CTYRAG 3 cut(s) 131, 533, 900
Sse9I AATT 8 cut(s) 31, 106, 383, 415, 791, 810, 888, 993
SseBI AGGCCT 2 cut(s) 233, 331
SspDI GGCGCC 1 cut(s) 362
SspMI CTAG 1 cut(s) 555
StuI AGGCCT 2 cut(s) 233, 331
StyD4I CCNGG 4 cut(s) 432, 515, 671, 864
StyI CCWWGG 3 cut(s) 204, 219, 326
TaaI ACNGT 3 cut(s) 471, 778, 788
TaiI ACGT 3 cut(s) 243, 315, 581
TaqI TCGA 3 cut(s) 162, 198, 685
TasI AATT 8 cut(s) 31, 106, 383, 415, 791, 810, 888, 993
TatI WGTACW 2 cut(s) 557, 999
TauI GCSGC 6 cut(s) 173, 368, 371, 374, 377, 607
TfiI GAWTC 3 cut(s) 285, 437, 755
Tru1I TTAA 5 cut(s) 429, 491, 911, 984, 992
Tru9I TTAA 5 cut(s) 429, 491, 911, 984, 992
TscAI CASTG 2 cut(s) 474, 781
TseFI GTSAC 2 cut(s) 241, 769
TseI GCWGC 1 cut(s) 730
Tsp45I GTSAC 2 cut(s) 241, 769
TspDTI ATGAA 2 cut(s) 115, 578
TspGWI ACGGA 4 cut(s) 260, 395, 526, 646
TspRI CASTG 2 cut(s) 474, 781
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 513
XapI RAATTY 2 cut(s) 31, 106
XspI CTAG 1 cut(s) 555
ZrmI AGTACT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.