Rh6AG199000

5-formyltetrahydrofolate cyclo-ligase-like protein COG0212

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009639
Physical Location & Seq
Reverse (-)
35830004 .. 35832896
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG199000.1

Sequence Viewer

Length: 951 bp
ATGCTCAAAACTAGCCGAAACGACGTCGGCCCCTTCGACGACGCAGCTTACGAGGCCGAGCGGCGCAGCCTCGACGCCAAGGCTCGGCAAACCATGGCTGAGGAGGCTTCCAATGTTGCGGAAGACCCCAAAGCTTGGAAATGGGTGATTCGGAAGAGAATTTGGGACATGATGGAGGCCCGCAACATAGCCCAGAACCCAAGGCCTGTTCACCACCGCATCCCCAACTTCGTCGGCGCCTCCGCTGCTGCCGATAAATTGGGCGGGTTGGAAGCGTTCCGCGTCGGGAACTGTGTGAAAGTGAACCCGGATTCTCCTCAGAAGCAAGTCAGGTTTCTCACGCTCTCTGATGGGAAGAAGCTGTTGACTCCTCAACCCCGTTTACGGACTGGGTTTTTCTCTGTGATTGAATCTGAAATGTTGACTCCTAGTACTATGAAAGAGGCTTGCACCTCTGTTGGGGTGGCCAAACACGGGCGGCCTATTGGATTAGATGAGAAAATCAAGGTTGATCTTATTGTCATTGGCTCTGTTGCTGTTGATCCCAGGACAGGTGCTCGACTTGGCAAGGGTGAGGGATTTGCAGAGCTTGAATATGGGATGCTGCGATACATGGGAGCCATTGATGATTCAACACCAGTTGTCACTACTGTGCATGACAGTCAATTAGTAGATGATATTCCAATTGAGAAGCTGTTAGTCCATGATGTACCAGTTGACATCATATGCACTCCAACCCAGGTCATTTTTACCAACACATCAATTCCAAAGCCTCAAGGGATTTACTGGGACAAATTGTCTCCGGAGAAGCTGGGGCAAATTAAAATACTCAGAGAGCTGAAGAGAAGGATTGAACAAGAGACTGGACAAAAATTGCCATGCGGTCCATCAGAGAAACTGCCTCCCACAGCGCGAAAAGGAGCTAACTTGGTAGGTGGTAAAAGGATATAA

Protein Analysis

316

Amino Acids

34.91

Weight (kDa)

9.16

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 47 - 245 5.3e-35 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 61
AccII CGCG 2 cut(s) 282, 913
AccIII TCCGGA 1 cut(s) 802
AciI CCGC 9 cut(s) 61, 119, 181, 217, 243, 264, 280, 478, 882
AclWI GGATC 1 cut(s) 536
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 860
AcyI GRCGYC 3 cut(s) 24, 75, 237
AfaI GTAC 2 cut(s) 433, 711
AfiI CCNNNNNNNGG 6 cut(s) 84, 135, 384, 459, 474, 551
AgsI TTSAA 4 cut(s) 410, 593, 633, 854
AhdI GACNNNNNGTC 1 cut(s) 796
AjnI CCWGG 2 cut(s) 545, 738
AleI CACNNNNGTG 1 cut(s) 650
AluBI AGCT 8 cut(s) 47, 134, 361, 589, 694, 811, 838, 923
AluI AGCT 8 cut(s) 47, 134, 361, 589, 694, 811, 838, 923
Alw21I GWGCWC 1 cut(s) 559
Alw26I GTCTC 2 cut(s) 804, 854
AlwI GGATC 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 802
AoxI GGCC 6 cut(s) 28, 54, 177, 203, 465, 479
ApeKI GCWGC 5 cut(s) 44, 66, 245, 248, 604
ApoI RAATTY 1 cut(s) 159
AspLEI GCGC 3 cut(s) 66, 239, 913
AspS9I GGNCC 3 cut(s) 29, 178, 884
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 3 cut(s) 157, 203, 584
AvaII GGWCC 1 cut(s) 884
BalI TGGCCA 1 cut(s) 467
BanI GGYRCC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 559
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 56, 78, 232, 235, 591
BccI CCATC 3 cut(s) 166, 344, 895
BciT130I CCWGG 2 cut(s) 547, 740
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 2 cut(s) 804, 854
BfaI CTAG 2 cut(s) 12, 429
BfoI RGCGCY 1 cut(s) 240
BisI GCNGC 7 cut(s) 45, 62, 67, 246, 249, 479, 605
BlsI GCNGC 7 cut(s) 46, 63, 68, 247, 250, 480, 606
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 3 cut(s) 308, 547, 740
Bme18I GGWCC 1 cut(s) 884
BmeRI GACNNNNNGTC 1 cut(s) 796
BmgT120I GGNCC 3 cut(s) 29, 178, 884
BmiI GGNNCC 3 cut(s) 31, 238, 619
BmrFI CCNGG 3 cut(s) 308, 547, 740
BmrI ACTGGG 2 cut(s) 399, 796
BmsI GCATC 2 cut(s) 228, 591
BmuI ACTGGG 2 cut(s) 399, 796
BpiI GAAGAC 1 cut(s) 129
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 308
BsaHI GRCGYC 3 cut(s) 24, 75, 237
BsaJI CCNNGG 5 cut(s) 78, 93, 200, 545, 738
BsaWI WCCGGW 1 cut(s) 802
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 135, 384, 459, 474, 551
Bse1I ACTGG 5 cut(s) 394, 638, 713, 791, 868
BseAI TCCGGA 1 cut(s) 802
BseBI CCWGG 2 cut(s) 547, 740
BseDI CCNNGG 5 cut(s) 78, 93, 200, 545, 738
BseGI GGATG 2 cut(s) 219, 606
BseLI CCNNNNNNNGG 6 cut(s) 84, 135, 384, 459, 474, 551
BseMII CTCAG 3 cut(s) 90, 332, 844
BseNI ACTGG 5 cut(s) 394, 638, 713, 791, 868
BseRI GAGGAG 3 cut(s) 116, 306, 360
BseXI GCAGC 5 cut(s) 56, 78, 232, 235, 591
BseYI CCCAGC 1 cut(s) 811
Bsh1236I CGCG 2 cut(s) 282, 913
BshFI GGCC 6 cut(s) 30, 56, 179, 205, 467, 481
BshNI GGYRCC 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 559
BsiSI CCGG 2 cut(s) 308, 803
BslFI GGGAC 2 cut(s) 179, 803
BslI CCNNNNNNNGG 6 cut(s) 84, 135, 384, 459, 474, 551
BsmAI GTCTC 2 cut(s) 804, 854
BsmFI GGGAC 2 cut(s) 179, 803
BsnI GGCC 6 cut(s) 30, 56, 179, 205, 467, 481
Bsp1286I GDGCHC 1 cut(s) 559
Bsp13I TCCGGA 1 cut(s) 802
Bsp143I GATC 2 cut(s) 511, 541
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 9 cut(s) 61, 119, 181, 217, 243, 264, 280, 478, 882
BspANI GGCC 6 cut(s) 30, 56, 179, 205, 467, 481
BspCNI CTCAG 3 cut(s) 91, 331, 843
BspEI TCCGGA 1 cut(s) 802
BspFNI CGCG 2 cut(s) 282, 913
BspLI GGNNCC 3 cut(s) 31, 238, 619
BspPI GGATC 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 236
BsrBI CCGCTC 1 cut(s) 61
BsrI ACTGG 5 cut(s) 394, 638, 713, 791, 868
BssECI CCNNGG 5 cut(s) 78, 93, 200, 545, 738
BssMI GATC 2 cut(s) 511, 541
BssNI GRCGYC 3 cut(s) 24, 75, 237
BssT1I CCWWGG 3 cut(s) 78, 93, 200
Bst2UI CCWGG 2 cut(s) 547, 740
Bst4CI ACNGT 3 cut(s) 293, 652, 662
Bst6I CTCTTC 2 cut(s) 149, 836
BstACI GRCGYC 3 cut(s) 24, 75, 237
BstC8I GCNNGC 2 cut(s) 181, 448
BstDEI CTNAG 3 cut(s) 99, 318, 830
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 2 cut(s) 219, 606
BstFNI CGCG 2 cut(s) 282, 913
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 3 cut(s) 66, 239, 913
BstKTI GATC 2 cut(s) 514, 544
BstMAI GTCTC 2 cut(s) 804, 854
BstMBI GATC 2 cut(s) 511, 541
BstMWI GCNNNNNNNGC 3 cut(s) 53, 104, 245
BstNI CCWGG 2 cut(s) 547, 740
BstSCI CCNGG 3 cut(s) 306, 545, 738
BstUI CGCG 2 cut(s) 282, 913
BstV1I GCAGC 5 cut(s) 56, 78, 232, 235, 591
BstV2I GAAGAC 1 cut(s) 129
BsuRI GGCC 6 cut(s) 30, 56, 179, 205, 467, 481
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 219, 606
Cac8I GCNNGC 2 cut(s) 181, 448
CfoI GCGC 3 cut(s) 66, 239, 913
Cfr13I GGNCC 3 cut(s) 29, 178, 884
CseI GACGC 3 cut(s) 50, 83, 271
Csp6I GTAC 2 cut(s) 432, 710
CviAII CATG 6 cut(s) 94, 169, 613, 656, 704, 879
CviQI GTAC 2 cut(s) 432, 710
DdeI CTNAG 3 cut(s) 99, 318, 830
DinI GGCGCC 1 cut(s) 238
DpnI GATC 2 cut(s) 513, 543
DpnII GATC 2 cut(s) 511, 541
DriI GACNNNNNGTC 1 cut(s) 796
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 149, 836
Eam1105I GACNNNNNGTC 1 cut(s) 796
EarI CTCTTC 2 cut(s) 149, 836
Eco130I CCWWGG 3 cut(s) 78, 93, 200
Eco147I AGGCCT 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 884
Eco57I CTGAAG 1 cut(s) 860
EcoRII CCWGG 2 cut(s) 545, 738
EcoT14I CCWWGG 3 cut(s) 78, 93, 200
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 3 cut(s) 78, 93, 200
FaeI CATG 6 cut(s) 97, 172, 616, 659, 707, 882
FaqI GGGAC 2 cut(s) 179, 803
FatI CATG 6 cut(s) 93, 168, 612, 655, 703, 878
FauI CCCGC 2 cut(s) 188, 257
FauNDI CATATG 1 cut(s) 725
Fnu4HI GCNGC 7 cut(s) 45, 62, 67, 246, 249, 479, 605
FokI GGATG 2 cut(s) 206, 613
Fsp4HI GCNGC 7 cut(s) 45, 62, 67, 246, 249, 479, 605
FspBI CTAG 2 cut(s) 12, 429
GlaI GCGC 3 cut(s) 65, 238, 912
GluI GCNGC 7 cut(s) 45, 62, 67, 246, 249, 479, 605
GsaI CCCAGC 1 cut(s) 815
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 6 cut(s) 30, 56, 179, 205, 467, 481
HapII CCGG 2 cut(s) 308, 803
HgaI GACGC 3 cut(s) 50, 83, 271
HhaI GCGC 3 cut(s) 66, 239, 913
Hin1I GRCGYC 3 cut(s) 24, 75, 237
Hin1II CATG 6 cut(s) 97, 172, 616, 659, 707, 882
Hin6I GCGC 3 cut(s) 64, 237, 911
HinP1I GCGC 3 cut(s) 64, 237, 911
HincII GTYRAC 3 cut(s) 366, 423, 718
HindII GTYRAC 3 cut(s) 366, 423, 718
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 6 cut(s) 148, 311, 367, 410, 424, 629
HpaII CCGG 2 cut(s) 308, 803
HphI GGTGA 3 cut(s) 157, 203, 584
Hpy166II GTNNAC 6 cut(s) 211, 304, 366, 383, 423, 718
Hpy188I TCNGA 6 cut(s) 153, 321, 349, 415, 833, 892
Hpy188III TCNNGA 2 cut(s) 286, 803
Hpy8I GTNNAC 6 cut(s) 211, 304, 366, 383, 423, 718
Hpy99I CGWCG 7 cut(s) 26, 29, 41, 44, 77, 236, 287
HpyAV CCTTC 2 cut(s) 43, 840
HpyCH4III ACNGT 3 cut(s) 293, 652, 662
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 4 cut(s) 450, 584, 655, 729
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 104, 245
HpyF3I CTNAG 3 cut(s) 99, 318, 830
HpySE526I ACGT 1 cut(s) 24
Hsp92I GRCGYC 3 cut(s) 24, 75, 237
Hsp92II CATG 6 cut(s) 97, 172, 616, 659, 707, 882
HspAI GCGC 3 cut(s) 64, 237, 911
KasI GGCGCC 1 cut(s) 236
Kpn2I TCCGGA 1 cut(s) 802
Kzo9I GATC 2 cut(s) 511, 541
LmnI GCTCC 2 cut(s) 617, 920
Lsp1109I GCAGC 5 cut(s) 56, 78, 232, 235, 591
LweI GCATC 2 cut(s) 228, 591
MaeI CTAG 2 cut(s) 12, 429
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 643
MalI GATC 2 cut(s) 513, 543
MbiI CCGCTC 1 cut(s) 61
MboI GATC 2 cut(s) 511, 541
MboII GAAGA 4 cut(s) 134, 166, 367, 853
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 1 cut(s) 559
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 8 cut(s) 159, 257, 665, 684, 762, 794, 819, 872
MluNI TGGCCA 1 cut(s) 467
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 2 cut(s) 361, 418
MmeI TCCRAC 2 cut(s) 249, 758
Mox20I TGGCCA 1 cut(s) 467
MroI TCCGGA 1 cut(s) 802
MscI TGGCCA 1 cut(s) 467
MseI TTAA 1 cut(s) 822
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 308, 803
MspR9I CCNGG 3 cut(s) 308, 547, 740
MunI CAATTG 1 cut(s) 684
MvaI CCWGG 2 cut(s) 547, 740
MvnI CGCG 2 cut(s) 282, 913
MwoI GCNNNNNNNGC 3 cut(s) 53, 104, 245
NarI GGCGCC 1 cut(s) 237
NciI CCSGG 1 cut(s) 308
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 725
NdeII GATC 2 cut(s) 511, 541
NlaIII CATG 6 cut(s) 97, 172, 616, 659, 707, 882
NlaIV GGNNCC 3 cut(s) 31, 238, 619
NmeAIII GCCGAG 2 cut(s) 64, 82
NmuCI GTSAC 1 cut(s) 643
OliI CACNNNNGTG 1 cut(s) 650
PceI AGGCCT 1 cut(s) 205
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 48
PfeI GAWTC 4 cut(s) 148, 311, 410, 629
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 7 cut(s) 46, 63, 68, 247, 250, 480, 606
PleI GAGTC 2 cut(s) 361, 418
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 2 cut(s) 361, 418
Psp6I CCWGG 2 cut(s) 545, 738
PspFI CCCAGC 1 cut(s) 811
PspGI CCWGG 2 cut(s) 545, 738
PspN4I GGNNCC 3 cut(s) 31, 238, 619
PspPI GGNCC 3 cut(s) 29, 178, 884
RsaI GTAC 2 cut(s) 433, 711
RsaNI GTAC 2 cut(s) 432, 710
RseI CAYNNNNRTG 1 cut(s) 650
SaqAI TTAA 1 cut(s) 822
SatI GCNGC 7 cut(s) 45, 62, 67, 246, 249, 479, 605
Sau3AI GATC 2 cut(s) 511, 541
Sau96I GGNCC 3 cut(s) 29, 178, 884
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 2 cut(s) 361, 418
ScrFI CCNGG 3 cut(s) 308, 547, 740
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 2 cut(s) 228, 591
SfoI GGCGCC 1 cut(s) 238
SinI GGWCC 1 cut(s) 884
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 1 cut(s) 774
SmoI CTYRAG 1 cut(s) 774
Sse9I AATT 8 cut(s) 159, 257, 665, 684, 762, 794, 819, 872
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 9 cut(s) 61, 119, 181, 217, 243, 264, 280, 478, 882
SspDI GGCGCC 1 cut(s) 236
SspMI CTAG 2 cut(s) 12, 429
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 3 cut(s) 306, 545, 738
StyI CCWWGG 3 cut(s) 78, 93, 200
TaaI ACNGT 3 cut(s) 293, 652, 662
TaiI ACGT 1 cut(s) 27
TaqI TCGA 3 cut(s) 36, 72, 559
TasI AATT 8 cut(s) 159, 257, 665, 684, 762, 794, 819, 872
TatI WGTACW 1 cut(s) 431
TauI GCSGC 2 cut(s) 64, 481
TfiI GAWTC 4 cut(s) 148, 311, 410, 629
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 5 cut(s) 44, 66, 245, 248, 604
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 1 cut(s) 452
TspGWI ACGGA 1 cut(s) 400
Van91I CCANNNNNTGG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 884
XapI RAATTY 1 cut(s) 159
XspI CTAG 2 cut(s) 12, 429
ZraI GACGTC 1 cut(s) 25
ZrmI AGTACT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.