Rw6G017300

5-formyltetrahydrofolate cyclo-ligase-like protein COG0212

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009639
Physical Location & Seq
Reverse (-)
34352783 .. 34355856
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017300.1

Sequence Viewer

Length: 1065 bp
ATGGATTCACGACGTCTGCTCCGGAATTCACACCCTTTTACATTTACACTCGCTCCACCCATCACCAAAACCCGCCATTACTCACTCTCTCTCCCCTCCCGCAAACTCTTCTTCATGCTCAAAACTAGCCGAAACGACGTCGGCCCCTTCGACGACGCAGCTTACGAGGCCGAGCGGCGCAGCCTCGACGCCAAGGCTCGGCAAACCATGGCTGAGGAGGCTTCCAATGTTGCGGAAGACCCCAAAGCTTGGAAATGGGTGATTCGGAAGAGAATTTGGGACATGATGGAGGCCCGCAACATAGCCCAGAACCCAAGGCCTGTTCACCACCGCATCCCCAACTTCGTCGGCGCCTCCGCTGCTGCCGATAAATTGGGCGGGTTGGAAGCGTTCCGCGTCGGGAACTGTGTGAAAGTGAACCCGGATTCTCCTCAGAAGCAAGTCAGGTTTCTCACGCTCTCTGATGGGAAGAAGCTGTTGACTCCTCAACCCCGTTTACGGACTGGGTTTTTCTCTGTGATTGAATCTGAAATGTTGACTCCTAGTACTATGAAAGAGGCTTGCACCTCTGTTGGGGTGGCCAAACACGGGCGGCCTATTGGATTAGATGAGAAAATCAAGGTTGATCTTATTGTCATTGGCTCTGTTGCTGTTGATCCCAGGACAGGTGCTCGACTTGGCAAGGGTGAGGGATTTGCAGAGCTTGAATATGGGATGCTGCGATACATGGGAGCCATTGATGATTCAACACCAGTTGTCACTACTGTGCATGACAGTCAATTAGTAGATGATATTCCAATTGAGAAGCTGTTAGTCCATGATGTACCAGTTGACATCATATGCACTCCAACCCAGGTCATTTTTACCAACACATCAATTCCAAAGCCTCAAGGGATTTACTGGGACAAATTGTCTCCGGAGAAGCTGGGGCAAATTAAAATACTCAGAGAGCTGAAGAGAAGGATTGAACGAGAGACTGGACAAAAATTGCCATGCGGTCCATCGGAGAAACTGCCTCCCACAGCGCGAAGAGGAGCTAACTTGGTAGGTGGTAAAAGGATATAA

Protein Analysis

354

Amino Acids

39.48

Weight (kDa)

9.65

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5-FTHF_cyc-lig PF01812 85 - 283 7.2e-35 5-formyltetrahydrofolate cyclo-ligase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 16, 141
AccB1I GGYRCC 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 249
AccBSI CCGCTC 1 cut(s) 175
AccII CGCG 2 cut(s) 396, 1027
AccIII TCCGGA 2 cut(s) 21, 916
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 579
AcsI RAATTY 2 cut(s) 25, 273
AcuI CTGAAG 1 cut(s) 974
AcyI GRCGYC 4 cut(s) 13, 138, 189, 351
AfaI GTAC 2 cut(s) 547, 825
AfiI CCNNNNNNNGG 6 cut(s) 198, 249, 498, 573, 588, 665
AgsI TTSAA 4 cut(s) 524, 707, 747, 968
AhdI GACNNNNNGTC 1 cut(s) 910
AjnI CCWGG 2 cut(s) 659, 852
AleI CACNNNNGTG 1 cut(s) 764
AluBI AGCT 8 cut(s) 161, 248, 475, 703, 808, 925, 952, 1037
AluI AGCT 8 cut(s) 161, 248, 475, 703, 808, 925, 952, 1037
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 2 cut(s) 918, 968
AlwI GGATC 1 cut(s) 650
Aor13HI TCCGGA 2 cut(s) 21, 916
AoxI GGCC 6 cut(s) 142, 168, 291, 317, 579, 593
ApeKI GCWGC 5 cut(s) 158, 180, 359, 362, 718
ApoI RAATTY 2 cut(s) 25, 273
AspLEI GCGC 3 cut(s) 180, 353, 1027
AspS9I GGNCC 3 cut(s) 143, 292, 998
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 4 cut(s) 55, 271, 317, 698
AvaII GGWCC 1 cut(s) 998
BalI TGGCCA 1 cut(s) 581
BanI GGYRCC 1 cut(s) 350
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 673
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 5 cut(s) 170, 192, 346, 349, 705
BccI CCATC 4 cut(s) 68, 280, 458, 1009
BciT130I CCWGG 2 cut(s) 661, 854
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 918, 968
BfaI CTAG 2 cut(s) 126, 543
BfoI RGCGCY 1 cut(s) 354
BisI GCNGC 7 cut(s) 159, 176, 181, 360, 363, 593, 719
BlsI GCNGC 7 cut(s) 160, 177, 182, 361, 364, 594, 720
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 3 cut(s) 422, 661, 854
Bme18I GGWCC 1 cut(s) 998
BmeRI GACNNNNNGTC 1 cut(s) 910
BmgT120I GGNCC 3 cut(s) 143, 292, 998
BmiI GGNNCC 3 cut(s) 145, 352, 733
BmrFI CCNGG 3 cut(s) 422, 661, 854
BmrI ACTGGG 2 cut(s) 513, 910
BmsI GCATC 2 cut(s) 342, 705
BmuI ACTGGG 2 cut(s) 513, 910
BpiI GAAGAC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 873
BpuMI CCSGG 1 cut(s) 422
BsaHI GRCGYC 4 cut(s) 13, 138, 189, 351
BsaJI CCNNGG 5 cut(s) 192, 207, 314, 659, 852
BsaWI WCCGGW 2 cut(s) 21, 916
BsaXI ACNNNNNCTCC 5 cut(s) 33, 37, 67, 75, 105
Bsc4I CCNNNNNNNGG 6 cut(s) 198, 249, 498, 573, 588, 665
Bse1I ACTGG 5 cut(s) 508, 752, 827, 905, 982
BseAI TCCGGA 2 cut(s) 21, 916
BseBI CCWGG 2 cut(s) 661, 854
BseDI CCNNGG 5 cut(s) 192, 207, 314, 659, 852
BseGI GGATG 2 cut(s) 333, 720
BseLI CCNNNNNNNGG 6 cut(s) 198, 249, 498, 573, 588, 665
BseMII CTCAG 3 cut(s) 204, 446, 958
BseNI ACTGG 5 cut(s) 508, 752, 827, 905, 982
BseRI GAGGAG 4 cut(s) 230, 420, 474, 1047
BseXI GCAGC 5 cut(s) 170, 192, 346, 349, 705
BseYI CCCAGC 1 cut(s) 925
Bsh1236I CGCG 2 cut(s) 396, 1027
BshFI GGCC 6 cut(s) 144, 170, 293, 319, 581, 595
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 673
BsiSI CCGG 3 cut(s) 22, 422, 917
BslFI GGGAC 2 cut(s) 293, 917
BslI CCNNNNNNNGG 6 cut(s) 198, 249, 498, 573, 588, 665
BsmAI GTCTC 2 cut(s) 918, 968
BsmFI GGGAC 2 cut(s) 293, 917
BsnI GGCC 6 cut(s) 144, 170, 293, 319, 581, 595
Bsp1286I GDGCHC 1 cut(s) 673
Bsp13I TCCGGA 2 cut(s) 21, 916
Bsp143I GATC 2 cut(s) 625, 655
Bsp19I CCATGG 1 cut(s) 207
BspANI GGCC 6 cut(s) 144, 170, 293, 319, 581, 595
BspCNI CTCAG 3 cut(s) 205, 445, 957
BspEI TCCGGA 2 cut(s) 21, 916
BspFNI CGCG 2 cut(s) 396, 1027
BspLI GGNNCC 3 cut(s) 145, 352, 733
BspPI GGATC 1 cut(s) 650
BspT107I GGYRCC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 175
BsrI ACTGG 5 cut(s) 508, 752, 827, 905, 982
BssECI CCNNGG 5 cut(s) 192, 207, 314, 659, 852
BssMI GATC 2 cut(s) 625, 655
BssNI GRCGYC 4 cut(s) 13, 138, 189, 351
BssT1I CCWWGG 3 cut(s) 192, 207, 314
Bst2UI CCWGG 2 cut(s) 661, 854
Bst4CI ACNGT 3 cut(s) 407, 766, 776
Bst6I CTCTTC 4 cut(s) 113, 263, 950, 1024
BstACI GRCGYC 4 cut(s) 13, 138, 189, 351
BstC8I GCNNGC 2 cut(s) 295, 562
BstDEI CTNAG 3 cut(s) 213, 432, 944
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 333, 720
BstFNI CGCG 2 cut(s) 396, 1027
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 3 cut(s) 180, 353, 1027
BstKTI GATC 2 cut(s) 628, 658
BstMAI GTCTC 2 cut(s) 918, 968
BstMBI GATC 2 cut(s) 625, 655
BstMWI GCNNNNNNNGC 3 cut(s) 167, 218, 359
BstNI CCWGG 2 cut(s) 661, 854
BstSCI CCNGG 3 cut(s) 420, 659, 852
BstUI CGCG 2 cut(s) 396, 1027
BstV1I GCAGC 5 cut(s) 170, 192, 346, 349, 705
BstV2I GAAGAC 1 cut(s) 243
BsuRI GGCC 6 cut(s) 144, 170, 293, 319, 581, 595
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 333, 720
Cac8I GCNNGC 2 cut(s) 295, 562
CfoI GCGC 3 cut(s) 180, 353, 1027
Cfr13I GGNCC 3 cut(s) 143, 292, 998
CseI GACGC 3 cut(s) 164, 197, 385
Csp6I GTAC 2 cut(s) 546, 824
CviAII CATG 7 cut(s) 115, 208, 283, 727, 770, 818, 993
CviQI GTAC 2 cut(s) 546, 824
DdeI CTNAG 3 cut(s) 213, 432, 944
DinI GGCGCC 1 cut(s) 352
DpnI GATC 2 cut(s) 627, 657
DpnII GATC 2 cut(s) 625, 655
DriI GACNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 579
Eam1104I CTCTTC 4 cut(s) 113, 263, 950, 1024
Eam1105I GACNNNNNGTC 1 cut(s) 910
EarI CTCTTC 4 cut(s) 113, 263, 950, 1024
Eco130I CCWWGG 3 cut(s) 192, 207, 314
Eco147I AGGCCT 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 998
Eco57I CTGAAG 1 cut(s) 974
EcoRI GAATTC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 659, 852
EcoT14I CCWWGG 3 cut(s) 192, 207, 314
EgeI GGCGCC 1 cut(s) 352
EheI GGCGCC 1 cut(s) 352
ErhI CCWWGG 3 cut(s) 192, 207, 314
FaeI CATG 7 cut(s) 118, 211, 286, 730, 773, 821, 996
FaqI GGGAC 2 cut(s) 293, 917
FatI CATG 7 cut(s) 114, 207, 282, 726, 769, 817, 992
FauI CCCGC 4 cut(s) 80, 107, 302, 371
FauNDI CATATG 1 cut(s) 839
Fnu4HI GCNGC 7 cut(s) 159, 176, 181, 360, 363, 593, 719
FokI GGATG 2 cut(s) 320, 727
Fsp4HI GCNGC 7 cut(s) 159, 176, 181, 360, 363, 593, 719
FspBI CTAG 2 cut(s) 126, 543
GlaI GCGC 3 cut(s) 179, 352, 1026
GluI GCNGC 7 cut(s) 159, 176, 181, 360, 363, 593, 719
GsaI CCCAGC 1 cut(s) 929
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 6 cut(s) 144, 170, 293, 319, 581, 595
HapII CCGG 3 cut(s) 22, 422, 917
HgaI GACGC 3 cut(s) 164, 197, 385
HhaI GCGC 3 cut(s) 180, 353, 1027
Hin1I GRCGYC 4 cut(s) 13, 138, 189, 351
Hin1II CATG 7 cut(s) 118, 211, 286, 730, 773, 821, 996
Hin6I GCGC 3 cut(s) 178, 351, 1025
HinP1I GCGC 3 cut(s) 178, 351, 1025
HincII GTYRAC 3 cut(s) 480, 537, 832
HindII GTYRAC 3 cut(s) 480, 537, 832
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 7 cut(s) 5, 262, 425, 481, 524, 538, 743
HpaII CCGG 3 cut(s) 22, 422, 917
HphI GGTGA 4 cut(s) 55, 271, 317, 698
Hpy166II GTNNAC 6 cut(s) 325, 418, 480, 497, 537, 832
Hpy188I TCNGA 6 cut(s) 267, 435, 463, 529, 947, 1006
Hpy188III TCNNGA 4 cut(s) 9, 22, 400, 917
Hpy8I GTNNAC 6 cut(s) 325, 418, 480, 497, 537, 832
Hpy99I CGWCG 8 cut(s) 15, 140, 143, 155, 158, 191, 350, 401
HpyAV CCTTC 2 cut(s) 157, 954
HpyCH4III ACNGT 3 cut(s) 407, 766, 776
HpyCH4IV ACGT 2 cut(s) 13, 138
HpyCH4V TGCA 4 cut(s) 564, 698, 769, 843
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 218, 359
HpyF3I CTNAG 3 cut(s) 213, 432, 944
HpySE526I ACGT 2 cut(s) 13, 138
Hsp92I GRCGYC 4 cut(s) 13, 138, 189, 351
Hsp92II CATG 7 cut(s) 118, 211, 286, 730, 773, 821, 996
HspAI GCGC 3 cut(s) 178, 351, 1025
KasI GGCGCC 1 cut(s) 350
Kpn2I TCCGGA 2 cut(s) 21, 916
Kzo9I GATC 2 cut(s) 625, 655
LmnI GCTCC 4 cut(s) 24, 58, 731, 1034
Lsp1109I GCAGC 5 cut(s) 170, 192, 346, 349, 705
LweI GCATC 2 cut(s) 342, 705
MaeI CTAG 2 cut(s) 126, 543
MaeII ACGT 2 cut(s) 13, 138
MaeIII GTNAC 1 cut(s) 757
MalI GATC 2 cut(s) 627, 657
MbiI CCGCTC 1 cut(s) 175
MboI GATC 2 cut(s) 625, 655
MboII GAAGA 7 cut(s) 100, 103, 248, 280, 481, 967, 1041
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 1 cut(s) 673
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 9 cut(s) 25, 273, 371, 779, 798, 876, 908, 933, 986
MluNI TGGCCA 1 cut(s) 581
Mly113I GGCGCC 1 cut(s) 351
MlyI GAGTC 2 cut(s) 475, 532
MmeI TCCRAC 2 cut(s) 363, 872
Mox20I TGGCCA 1 cut(s) 581
MroI TCCGGA 2 cut(s) 21, 916
MscI TGGCCA 1 cut(s) 581
MseI TTAA 1 cut(s) 936
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 1 cut(s) 581
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 3 cut(s) 22, 422, 917
MspR9I CCNGG 3 cut(s) 422, 661, 854
MunI CAATTG 1 cut(s) 798
MvaI CCWGG 2 cut(s) 661, 854
MvnI CGCG 2 cut(s) 396, 1027
MwoI GCNNNNNNNGC 3 cut(s) 167, 218, 359
NarI GGCGCC 1 cut(s) 351
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 839
NdeII GATC 2 cut(s) 625, 655
NlaIII CATG 7 cut(s) 118, 211, 286, 730, 773, 821, 996
NlaIV GGNNCC 3 cut(s) 145, 352, 733
NmeAIII GCCGAG 2 cut(s) 178, 196
NmuCI GTSAC 1 cut(s) 757
OliI CACNNNNGTG 1 cut(s) 764
PceI AGGCCT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 162
PfeI GAWTC 5 cut(s) 5, 262, 425, 524, 743
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 7 cut(s) 160, 177, 182, 361, 364, 594, 720
PleI GAGTC 2 cut(s) 475, 532
PluTI GGCGCC 1 cut(s) 354
PpsI GAGTC 2 cut(s) 475, 532
Psp6I CCWGG 2 cut(s) 659, 852
PspFI CCCAGC 1 cut(s) 925
PspGI CCWGG 2 cut(s) 659, 852
PspN4I GGNNCC 3 cut(s) 145, 352, 733
PspPI GGNCC 3 cut(s) 143, 292, 998
RsaI GTAC 2 cut(s) 547, 825
RsaNI GTAC 2 cut(s) 546, 824
RseI CAYNNNNRTG 1 cut(s) 764
SaqAI TTAA 1 cut(s) 936
SatI GCNGC 7 cut(s) 159, 176, 181, 360, 363, 593, 719
Sau3AI GATC 2 cut(s) 625, 655
Sau96I GGNCC 3 cut(s) 143, 292, 998
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 2 cut(s) 475, 532
ScrFI CCNGG 3 cut(s) 422, 661, 854
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 2 cut(s) 342, 705
SfoI GGCGCC 1 cut(s) 352
SinI GGWCC 1 cut(s) 998
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 888
SmoI CTYRAG 1 cut(s) 888
Sse9I AATT 9 cut(s) 25, 273, 371, 779, 798, 876, 908, 933, 986
SseBI AGGCCT 1 cut(s) 319
SspDI GGCGCC 1 cut(s) 350
SspMI CTAG 2 cut(s) 126, 543
StuI AGGCCT 1 cut(s) 319
StyD4I CCNGG 3 cut(s) 420, 659, 852
StyI CCWWGG 3 cut(s) 192, 207, 314
TaaI ACNGT 3 cut(s) 407, 766, 776
TaiI ACGT 2 cut(s) 16, 141
TaqI TCGA 3 cut(s) 150, 186, 673
TasI AATT 9 cut(s) 25, 273, 371, 779, 798, 876, 908, 933, 986
TatI WGTACW 1 cut(s) 545
TauI GCSGC 2 cut(s) 178, 595
TfiI GAWTC 5 cut(s) 5, 262, 425, 524, 743
Tru1I TTAA 1 cut(s) 936
Tru9I TTAA 1 cut(s) 936
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 5 cut(s) 158, 180, 359, 362, 718
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 2 cut(s) 103, 566
TspGWI ACGGA 1 cut(s) 514
Van91I CCANNNNNTGG 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 998
XapI RAATTY 2 cut(s) 25, 273
XspI CTAG 2 cut(s) 126, 543
ZraI GACGTC 2 cut(s) 14, 139
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.