AT1G76950

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009215
Physical Location & Seq
Forward (+)
28906441 .. 28911584
5144 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76950.1

Sequence Viewer

Length: 3312 bp
ATGGCAGATCTTGTGACCTATAGTAATGCCGACCACAACCTTGAACAGGCATTGATTACATTGAAGAAGGGTACTCAACTTTTGAAATATGGTCGCAAAGGGAAGCCCAAGTTTTATCCTTTCAGACTGTCTAGTGATGAAAAATCTTTGATCTGGATATCAAGTAGTGGTGAAAAGCGACTGAAGTTAGCATCTGTATCTAAAATTGTACCTGGGCAGAGAACTGCTGTTTTCCAGCGTTATCTTCGCCCAGAGAAAGATTACTTATCCTTTTCTCTCTTATACAACGGAAAAAAGAAGTCTCTTGACTTGATTTGTAAGGACAAGGTTGAGGCTGAGATTTGGATTGGAGGCCTGAAAACATTAATATCCACTGGGCAAGGCGGGCGCTCAAAAATCGATGGCTGGAGTGGCGGAGGCCTTTCAGTTGATGCCAGCAGAGAATTGACATCAAGCAGTCCAAGTAGCAGTTCTGCCAGTGCTTCACGGGGTCACAGCTCTCCTGGGACTCCCTTTAACATCGATCCAATTACTTCTCCTAAGAGTGCTGAGCCTGAGGTTCCTCCAACTGATTCAGAGAAATCACATGTTGCTTTGGACAACAAAAATATGCAAACAAAGGTATCTGGTTCAGATGGTTTTCGAGTTAGTGTCTCTAGTGCCCAAAGTAGTTCTAGTCATGGTTCTGCAGCAGATGACTCAGATGCTCTAGGTGATGTTTACATCTGGGGTGAGGTTATATGTGACAATGTTGTTAAGGTTGGGATTGATAAGAATGCAAGTTATCTGACTACAAGAACGGATGTCCTTGTGCCAAAGCCATTGGAGTCGAATATTGTCTTGGATGTTCATCAAATTGCATGTGGTGTCAGGCATGCTGCATTTGTGACAAGGCAAGGTGAAATTTTTACTTGGGGTGAAGAATCTGGAGGAAGACTGGGTCATGGTATTGGGAAAGATGTTTTTCATCCCCGTCTTGTTGAATCTCTCACAGCTACTTCATCTGTTGACTTTGTTGCTTGTGGAGAATTCCACACTTGTGCTGTTACACTGGCTGGAGAGCTCTATACCTGGGGTGACGGCACACATAATGTTGGGCTTCTTGGGCATGGCTCTGATATCAGTCACTGGATACCAAAGAGAATTGCCGGTTCTCTCGAGGGGCTTCATGTGGCTTCAGTAAGTTGTGGACCCTGGCACACCGCTTTGATAACATCATATGGGAGGCTTTTTACATTTGGAGATGGAACATTTGGTGTCTTAGGGCACGGGGACAAGGAAACAGTTCAGTATCCAAGAGAAGTTGAATCTCTATCAGGATTGAGAACGATTGCGGTTTCATGTGGGGTTTGGCACACTGCTGCTGTTGTTGAGATCATTGTCACACAGTCAAACTCAAGTTCTGTATCATCTGGAAAATTGTTCACATGGGGCGATGGAGACAAAAACCGTCTCGGACATGGTGACAAGGATCCTCGGCTTAAACCAACGTGTGTGCCTGCATTAATTGATTACAACTTTCACAAAATTGCATGTGGACACAGTTTGACAGTTGGTTTGACCACGTCTGGACAAGTGTTCACAATGGGGAGTACAGTTTATGGTCAACTTGGGAATTTACAGACTGACGGAAAATTACCGTGTTTGGTGGAAGACAAGCTTGCAAGTGAATTTGTCGAGGAGATCTCCTGCGGGGCATATCACGTGGCAGCCTTAACATCTAGAAATGAAGTTTACACATGGGGTAAAGGAGCCAATGGGAGATTAGGGCATGGTGATCTGGAGGATCGAAAAGTACCTACTATTGTGGAAGCTTTGAAGGATAGACACGTAAAATACATTGCTTGCGGATCGAATTACACTGCAGCCATATGTCTTCACAAATGGGTGTCTGGTGCTGAGCAGTCTCAGTGTTCAACCTGTAGACTGGCTTTTGGGTTTACACGGAAAAGACATAACTGTTACAATTGTGGACTTGTGCACTGTCATTCATGCAGTTCAAAAAAAGCATTCCGAGCTGCACTAGCCCCAAGCGCGGGAAGACTATATCGTGTCTGCGATTCTTGTTATGTTAAGCTCAGCAAAGTGTCTGAGATAAACGATACTAACAGAAGGAACAGTGCTGTGCCTCGTCTTTCAGGGGAGAATAGAGACAGATTAGATAAATCTGAAATAAGATTAGCTAAATTTGGTACATCCAATATGGACCTAATTAAGCAATTGGATAGCAAAGCGGCCAAACAAGGAAAGAAAACTGACACTTTTTCACTCGGTCGCAACTCTCAGCTGCCTTCTTTGTTGCAGCTGAAAGATGCTGTGCAGTCTAATATAGGCGATATGCGAAGAGCTACTCCAAAGTTAGCTCAGGCTCCATCAGGCATTAGCTCTCGGTCAGTGTCACCTTTCTCTAGGAGATCTAGTCCTCCTCGCTCTGCCACTCCTATGCCTTCAACTTCTGGGCTCTATTTCCCGGTGGGTATAGCAGATAATATGAAAAAAACCAATGAAATTCTGAACCAGGAGATCGTTAAGTTACGAACACAGGTTGACAGTCTAACTCAAAAATGTGAATTCCAAGAAGTAGAGCTTCAGAACTCTGTAAAGAAGACTCAGGAAGCCTTAGCATTAGCAGAGGAGGAATCTGCCAAATCAAGAGCCGCGAAAGAGGCAATCAAGTCGCTCATAGCGCAGCTCAAAGATGTCGCTGAGAAGTTACCTCCAGGTGAGAGTGTAAAACTCGCGTGCCTTCAAAACGGATTAGACCAGAATGGTTTCCATTTCCCTGAAGAAAATGGTTTCCATCCTTCAAGGTCGGAATCGATGACTTCATCTATATCTTCAGTTGCACCTTTTGACTTTGCCTTTGCAAATGCATCTTGGTCTAATCTCCAATCACCAAAGCAAACGCCTAGAGCCAGCGAAAGAAACAGCAATGCTTATCCAGCGGACCCACGGCTTAGCAGCAGCGGGTCAGTAATCAGTGAGAGAATCGAGCCTTTCCAGTTCCAGAACAACAGCGACAATGGTTCGAGTCAAACAGGAGTTAACAATACTAATCAGGTAGAAGCAGAGTGGATCGAACAGTACGAGCCAGGCGTGTATATAACCCTTGTTGCCCTCCACGATGGAACTAGAGATTTACGAAGAGTCCGCTTCAGCCGAAGAAGATTCGGGGAACATCAAGCTGAGACATGGTGGTCGGAGAATCGGGAAAAAGTGTACGAAAAATATAATGTAAGAGTATCGGAAAAATCAACGGCTTCTCAAACGCATAGAGACAGAGATGAAGAAGAAGAAGACATTCCTCACTAG

Protein Analysis

1103

Amino Acids

120.39

Weight (kDa)

8.48

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 15 - 123 6.1e-11 Pleckstrin homology domain
WD40_RLD PF25390 240 - 623 4.5e-76 RCC1-like domain
RCC1 PF00415 299 - 349 1.7e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 338 - 363 6.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 353 - 404 7.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 408 - 456 4.6e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 471 - 519 9.5e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 523 - 572 6.2e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 560 - 587 3.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 577 - 624 4.6e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 628 - 694 1.1e-13 FYVE zinc finger
BRX_N PF13713 879 - 908 1.3e-12 Transcription factor BRX N-terminal domain
BRX PF08381 1022 - 1077 6.1e-29 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3196
AccI GTMKAC 1 cut(s) 1924
AccII CGCG 3 cut(s) 2036, 2660, 2741
AclWI GGATC 6 cut(s) 518, 1466, 1479, 1794, 1858, 3083
AcoI YGGCCR 1 cut(s) 2235
AcsI RAATTY 7 cut(s) 903, 1028, 1615, 1670, 2186, 2508, 2570
AcuI CTGAAG 6 cut(s) 203, 1161, 2573, 2805, 2823, 3139
AcvI CACGTG 1 cut(s) 1705
AfaI GTAC 7 cut(s) 73, 210, 1594, 1797, 2194, 3086, 3221
AfiI CCNNNNNNNGG 3 cut(s) 46, 2035, 2036
AflIII ACRYGT 3 cut(s) 586, 1490, 1828
AjiI CACGTC 1 cut(s) 1566
AjnI CCWGG 7 cut(s) 211, 502, 1070, 1193, 2517, 2719, 3091
AjuI GAANNNNNNNTTGG 4 cut(s) 454, 486, 824, 856
AleI CACNNNNGTG 1 cut(s) 1038
Alw21I GWGCWC 2 cut(s) 1065, 1983
Alw26I GTCTC 8 cut(s) 306, 658, 1434, 1457, 1911, 2145, 3182, 3270
Alw44I GTGCAC 1 cut(s) 1979
AlwI GGATC 6 cut(s) 518, 1466, 1479, 1794, 1858, 3083
AlwNI CAGNNNCTG 1 cut(s) 1128
Ama87I CYCGRG 1 cut(s) 1157
AoxI GGCC 3 cut(s) 352, 418, 2235
ApaLI GTGCAC 1 cut(s) 1979
ApoI RAATTY 7 cut(s) 903, 1028, 1615, 1670, 2186, 2508, 2570
ArsI GACNNNNNNTTYG 4 cut(s) 1540, 1572, 2846, 2878
AseI ATTAAT 2 cut(s) 365, 1505
Asp700I GAANNNNTTC 3 cut(s) 1284, 2771, 3300
AspLEI GCGC 3 cut(s) 390, 2036, 2689
AspS9I GGNCC 3 cut(s) 1190, 2206, 2947
AsuC2I CCSGG 1 cut(s) 2471
AvaI CYCGRG 1 cut(s) 1157
AvaII GGWCC 3 cut(s) 1190, 2206, 2947
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 3 cut(s) 664, 1269, 1983
BaeI ACNNNNGTAYC 2 cut(s) 606, 639
BamHI GGATCC 1 cut(s) 1471
BanII GRGCYC 2 cut(s) 1065, 2463
BbrPI CACGTG 1 cut(s) 1705
BbsI GAAGAC 6 cut(s) 940, 1659, 1868, 2047, 2612, 3303
Bbv12I GWGCWC 2 cut(s) 1065, 1983
BccI CCATC 7 cut(s) 395, 629, 1238, 1430, 2380, 2808, 3119
BceAI ACGGC 3 cut(s) 1096, 2969, 3273
BcgI CGANNNNNNTGC 2 cut(s) 2658, 2692
BciT130I CCWGG 7 cut(s) 213, 504, 1072, 1195, 2519, 2721, 3093
BciVI GTATCC 2 cut(s) 1125, 1302
BcnI CCSGG 1 cut(s) 2471
BcoDI GTCTC 8 cut(s) 306, 658, 1434, 1457, 1911, 2145, 3182, 3270
BfmI CTRYAG 4 cut(s) 19, 687, 1863, 1921
BfoI RGCGCY 1 cut(s) 391
BfuI GTATCC 2 cut(s) 1125, 1302
BglII AGATCT 3 cut(s) 7, 1683, 2414
BlpI GCTNAGC 4 cut(s) 549, 1899, 2078, 2957
Bme1390I CCNGG 8 cut(s) 213, 504, 1072, 1195, 2471, 2519, 2721, 3093
Bme18I GGWCC 3 cut(s) 1190, 2206, 2947
BmeT110I CYCGRG 1 cut(s) 1157
BmgBI CACGTC 1 cut(s) 1566
BmgT120I GGNCC 3 cut(s) 1190, 2206, 2947
BmiI GGNNCC 6 cut(s) 561, 1192, 1473, 1753, 2370, 2949
BmrFI CCNGG 8 cut(s) 213, 504, 1072, 1195, 2471, 2519, 2721, 3093
BmrI ACTGGG 2 cut(s) 384, 947
BmsI GCATC 5 cut(s) 200, 421, 694, 2302, 2882
BmuI ACTGGG 2 cut(s) 384, 947
BpiI GAAGAC 6 cut(s) 940, 1659, 1868, 2047, 2612, 3303
BplI GAGNNNNNCTC 2 cut(s) 1670, 1702
BpmI CTGGAG 5 cut(s) 427, 948, 1077, 1802, 2703
Bpu10I CCTNAGC 2 cut(s) 2364, 2620
Bpu1102I GCTNAGC 4 cut(s) 549, 1899, 2078, 2957
BpuEI CTTGAG 1 cut(s) 1381
BpuMI CCSGG 1 cut(s) 2471
Bsa29I ATCGAT 3 cut(s) 399, 522, 2819
BsaAI YACGTR 2 cut(s) 1705, 1831
BsaBI GATNNNNATC 1 cut(s) 849
BsaJI CCNNGG 6 cut(s) 212, 503, 1071, 1193, 1475, 2951
BsaXI ACNNNNNCTCC 2 cut(s) 2404, 2434
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 2035, 2036
Bse118I RCCGGY 1 cut(s) 1148
Bse1I ACTGG 7 cut(s) 379, 477, 942, 1056, 1133, 1932, 3001
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 2 cut(s) 1839, 2938
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 7 cut(s) 213, 504, 1072, 1195, 2519, 2721, 3093
BseCI ATCGAT 3 cut(s) 399, 522, 2819
BseDI CCNNGG 6 cut(s) 212, 503, 1071, 1193, 1475, 2951
BseGI GGATG 5 cut(s) 808, 850, 967, 2195, 2800
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 3 cut(s) 46, 2035, 2036
BseMI GCAATG 2 cut(s) 1839, 2938
BseNI ACTGG 7 cut(s) 379, 477, 942, 1056, 1133, 1932, 3001
BseRI GAGGAG 3 cut(s) 1694, 2415, 2648
BseSI GKGCMC 3 cut(s) 664, 1269, 1983
BsgI GTGCAG 2 cut(s) 2004, 2339
Bsh1236I CGCG 3 cut(s) 2036, 2660, 2741
Bsh1285I CGRYCG 1 cut(s) 2275
BshFI GGCC 3 cut(s) 354, 420, 2237
BshVI ATCGAT 3 cut(s) 399, 522, 2819
BsiEI CGRYCG 1 cut(s) 2275
BsiHKAI GWGCWC 2 cut(s) 1065, 1983
BsiHKCI CYCGRG 1 cut(s) 1157
BsiSI CCGG 2 cut(s) 1149, 2471
BslFI GGGAC 2 cut(s) 520, 1286
BslI CCNNNNNNNGG 3 cut(s) 46, 2035, 2036
BsmAI GTCTC 8 cut(s) 306, 658, 1434, 1457, 1911, 2145, 3182, 3270
BsmBI CGTCTC 1 cut(s) 1457
BsmFI GGGAC 2 cut(s) 520, 1286
BsmI GAATGC 2 cut(s) 781, 2009
BsnI GGCC 3 cut(s) 354, 420, 2237
BsoBI CYCGRG 1 cut(s) 1157
Bsp1286I GDGCHC 5 cut(s) 664, 1065, 1269, 1983, 2463
Bsp1720I GCTNAGC 4 cut(s) 549, 1899, 2078, 2957
BspANI GGCC 3 cut(s) 354, 420, 2237
BspDI ATCGAT 3 cut(s) 399, 522, 2819
BspFNI CGCG 3 cut(s) 2036, 2660, 2741
BspLI GGNNCC 6 cut(s) 561, 1192, 1473, 1753, 2370, 2949
BspMAI CTGCAG 2 cut(s) 691, 1867
BspPI GGATC 6 cut(s) 518, 1466, 1479, 1794, 1858, 3083
BspQI GCTCTTC 1 cut(s) 2338
BsrDI GCAATG 2 cut(s) 1839, 2938
BsrFI RCCGGY 1 cut(s) 1148
BsrI ACTGG 7 cut(s) 379, 477, 942, 1056, 1133, 1932, 3001
BssAI RCCGGY 1 cut(s) 1148
BssECI CCNNGG 6 cut(s) 212, 503, 1071, 1193, 1475, 2951
Bst2UI CCWGG 7 cut(s) 213, 504, 1072, 1195, 2519, 2721, 3093
Bst6I CTCTTC 2 cut(s) 2338, 3139
BstBAI YACGTR 2 cut(s) 1705, 1831
BstC8I GCNNGC 8 cut(s) 386, 436, 876, 1500, 1662, 1846, 2743, 2917
BstDSI CCRYGG 1 cut(s) 2951
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 5 cut(s) 808, 850, 967, 2195, 2800
BstFNI CGCG 3 cut(s) 2036, 2660, 2741
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 3 cut(s) 390, 2036, 2689
BstMAI GTCTC 8 cut(s) 306, 658, 1434, 1457, 1911, 2145, 3182, 3270
BstMCI CGRYCG 1 cut(s) 2275
BstMWI GCNNNNNNNGC 9 cut(s) 385, 411, 1105, 2015, 2024, 2033, 2666, 2686, 2942
BstNI CCWGG 7 cut(s) 213, 504, 1072, 1195, 2519, 2721, 3093
BstNSI RCATGY 4 cut(s) 590, 864, 878, 1536
BstSCI CCNGG 8 cut(s) 211, 502, 1070, 1193, 2469, 2517, 2719, 3091
BstSFI CTRYAG 4 cut(s) 19, 687, 1863, 1921
BstSLI GKGCMC 3 cut(s) 664, 1269, 1983
BstUI CGCG 3 cut(s) 2036, 2660, 2741
BstV2I GAAGAC 6 cut(s) 940, 1659, 1868, 2047, 2612, 3303
BstX2I RGATCY 4 cut(s) 7, 1471, 1683, 2414
BstYI RGATCY 4 cut(s) 7, 1471, 1683, 2414
Bsu15I ATCGAT 3 cut(s) 399, 522, 2819
Bsu36I CCTNAGG 1 cut(s) 555
BsuI GTATCC 2 cut(s) 1125, 1302
BsuRI GGCC 3 cut(s) 354, 420, 2237
BsuTUI ATCGAT 3 cut(s) 399, 522, 2819
BtgI CCRYGG 1 cut(s) 2951
BtgZI GCGATG 1 cut(s) 1449
BtrI CACGTC 1 cut(s) 1566
BtsCI GGATG 5 cut(s) 808, 850, 967, 2195, 2800
BtsI GCAGTG 2 cut(s) 1356, 1860
Cac8I GCNNGC 8 cut(s) 386, 436, 876, 1500, 1662, 1846, 2743, 2917
CaiI CAGNNNCTG 1 cut(s) 1128
CfoI GCGC 3 cut(s) 390, 2036, 2689
Cfr10I RCCGGY 1 cut(s) 1148
Cfr13I GGNCC 3 cut(s) 1190, 2206, 2947
ClaI ATCGAT 3 cut(s) 399, 522, 2819
Csp6I GTAC 7 cut(s) 72, 209, 1593, 1796, 2193, 3085, 3220
CviQI GTAC 7 cut(s) 72, 209, 1593, 1796, 2193, 3085, 3220
DrdI GACNNNNNNGTC 1 cut(s) 3196
DseDI GACNNNNNNGTC 1 cut(s) 3196
EaeI YGGCCR 1 cut(s) 2235
Eam1104I CTCTTC 2 cut(s) 2338, 3139
EarI CTCTTC 2 cut(s) 2338, 3139
EciI GGCGGA 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 1063
Eco147I AGGCCT 2 cut(s) 354, 420
Eco24I GRGCYC 2 cut(s) 1065, 2463
Eco32I GATATC 2 cut(s) 159, 1120
Eco47I GGWCC 3 cut(s) 1190, 2206, 2947
Eco53kI GAGCTC 1 cut(s) 1063
Eco57I CTGAAG 6 cut(s) 203, 1161, 2573, 2805, 2823, 3139
Eco72I CACGTG 1 cut(s) 1705
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 1157
EcoICRI GAGCTC 1 cut(s) 1063
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoRI GAATTC 2 cut(s) 1028, 2570
EcoRII CCWGG 7 cut(s) 211, 502, 1070, 1193, 2517, 2719, 3091
EcoRV GATATC 2 cut(s) 159, 1120
EcoT22I ATGCAT 1 cut(s) 2875
EcoT38I GRGCYC 2 cut(s) 1065, 2463
Esp3I CGTCTC 1 cut(s) 1457
FalI AAGNNNNNCTT 4 cut(s) 1644, 1676, 2571, 2603
FaqI GGGAC 2 cut(s) 520, 1286
FauI CCCGC 4 cut(s) 377, 1685, 2029, 2960
FauNDI CATATG 2 cut(s) 1219, 1871
FblI GTMKAC 1 cut(s) 1924
FokI GGATG 5 cut(s) 815, 857, 954, 2182, 2787
FriOI GRGCYC 2 cut(s) 1065, 2463
GlaI GCGC 3 cut(s) 389, 2035, 2688
GsuI CTGGAG 5 cut(s) 427, 948, 1077, 1802, 2703
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 3 cut(s) 354, 420, 2237
HapII CCGG 2 cut(s) 1149, 2471
HhaI GCGC 3 cut(s) 390, 2036, 2689
Hin6I GCGC 3 cut(s) 388, 2034, 2687
HinP1I GCGC 3 cut(s) 388, 2034, 2687
HincII GTYRAC 4 cut(s) 1009, 1607, 2548, 3046
HindII GTYRAC 4 cut(s) 1009, 1607, 2548, 3046
HindIII AAGCTT 2 cut(s) 1658, 1812
HpaI GTTAAC 1 cut(s) 3046
HpaII CCGG 2 cut(s) 1149, 2471
HpyAV CCTTC 7 cut(s) 61, 1813, 2106, 2301, 2457, 2756, 2814
HpyCH4IV ACGT 4 cut(s) 1490, 1565, 1704, 1830
HpyF10VI GCNNNNNNNGC 9 cut(s) 385, 411, 1105, 2015, 2024, 2033, 2666, 2686, 2942
HpySE526I ACGT 4 cut(s) 1490, 1565, 1704, 1830
HspAI GCGC 3 cut(s) 388, 2034, 2687
KspAI GTTAAC 1 cut(s) 3046
LguI GCTCTTC 1 cut(s) 2338
LmnI GCTCC 2 cut(s) 1751, 2374
LweI GCATC 5 cut(s) 200, 421, 694, 2302, 2882
MaeII ACGT 4 cut(s) 1490, 1565, 1704, 1830
MfeI CAATTG 2 cut(s) 1966, 2219
MflI RGATCY 4 cut(s) 7, 1471, 1683, 2414
MhlI GDGCHC 5 cut(s) 664, 1065, 1269, 1983, 2463
MlyI GAGTC 6 cut(s) 502, 692, 836, 2602, 3040, 3156
MmeI TCCRAC 3 cut(s) 590, 2793, 3180
Mph1103I ATGCAT 1 cut(s) 2875
MroXI GAANNNNTTC 3 cut(s) 1284, 2771, 3300
MslI CAYNNNNRTG 2 cut(s) 1038, 2441
MspA1I CMGCKG 4 cut(s) 2287, 2305, 2945, 2967
MspI CCGG 2 cut(s) 1149, 2471
MspR9I CCNGG 8 cut(s) 213, 504, 1072, 1195, 2471, 2519, 2721, 3093
MunI CAATTG 2 cut(s) 1966, 2219
Mva1269I GAATGC 2 cut(s) 781, 2009
MvaI CCWGG 7 cut(s) 213, 504, 1072, 1195, 2519, 2721, 3093
MvnI CGCG 3 cut(s) 2036, 2660, 2741
MwoI GCNNNNNNNGC 9 cut(s) 385, 411, 1105, 2015, 2024, 2033, 2666, 2686, 2942
NciI CCSGG 1 cut(s) 2471
NdeI CATATG 2 cut(s) 1219, 1871
NlaIV GGNNCC 6 cut(s) 561, 1192, 1473, 1753, 2370, 2949
NmeAIII GCCGAG 1 cut(s) 1456
NmuCI GTSAC 9 cut(s) 13, 491, 743, 886, 1076, 1124, 1381, 1463, 2397
NsiI ATGCAT 1 cut(s) 2875
NspI RCATGY 4 cut(s) 590, 864, 878, 1536
OliI CACNNNNGTG 1 cut(s) 1038
PaeI GCATGC 1 cut(s) 878
PaeR7I CTCGAG 1 cut(s) 1157
PceI AGGCCT 2 cut(s) 354, 420
PciI ACATGT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 2338
PcsI WCGNNNNNNNCGW 2 cut(s) 3084, 3093
PctI GAATGC 2 cut(s) 781, 2009
PdmI GAANNNNTTC 3 cut(s) 1284, 2771, 3300
PflFI GACNNNGTC 2 cut(s) 939, 1564
PleI GAGTC 6 cut(s) 502, 692, 835, 2602, 3039, 3155
PmaCI CACGTG 1 cut(s) 1705
PmlI CACGTG 1 cut(s) 1705
PpsI GAGTC 6 cut(s) 502, 692, 835, 2602, 3039, 3155
Ppu21I YACGTR 2 cut(s) 1705, 1831
PscI ACATGT 1 cut(s) 586
PshBI ATTAAT 2 cut(s) 365, 1505
Psp124BI GAGCTC 1 cut(s) 1065
Psp6I CCWGG 7 cut(s) 211, 502, 1070, 1193, 2517, 2719, 3091
PspCI CACGTG 1 cut(s) 1705
PspGI CCWGG 7 cut(s) 211, 502, 1070, 1193, 2517, 2719, 3091
PspN4I GGNNCC 6 cut(s) 561, 1192, 1473, 1753, 2370, 2949
PspPI GGNCC 3 cut(s) 1190, 2206, 2947
PstI CTGCAG 2 cut(s) 691, 1867
PstNI CAGNNNCTG 1 cut(s) 1128
PsuI RGATCY 4 cut(s) 7, 1471, 1683, 2414
PsyI GACNNNGTC 2 cut(s) 939, 1564
PvuII CAGCTG 2 cut(s) 2287, 2305
RsaI GTAC 7 cut(s) 73, 210, 1594, 1797, 2194, 3086, 3221
RsaNI GTAC 7 cut(s) 72, 209, 1593, 1796, 2193, 3085, 3220
RseI CAYNNNNRTG 2 cut(s) 1038, 2441
SacI GAGCTC 1 cut(s) 1065
SapI GCTCTTC 1 cut(s) 2338
Sau96I GGNCC 3 cut(s) 1190, 2206, 2947
SchI GAGTC 6 cut(s) 502, 692, 836, 2602, 3040, 3156
ScrFI CCNGG 8 cut(s) 213, 504, 1072, 1195, 2471, 2519, 2721, 3093
SduI GDGCHC 5 cut(s) 664, 1065, 1269, 1983, 2463
SfaNI GCATC 5 cut(s) 200, 421, 694, 2302, 2882
SfcI CTRYAG 4 cut(s) 19, 687, 1863, 1921
Sfr274I CTCGAG 1 cut(s) 1157
SinI GGWCC 3 cut(s) 1190, 2206, 2947
SlaI CTCGAG 1 cut(s) 1157
SmiMI CAYNNNNRTG 2 cut(s) 1038, 2441
SmlI CTYRAG 2 cut(s) 1157, 1396
SmoI CTYRAG 2 cut(s) 1157, 1396
SphI GCATGC 1 cut(s) 878
SseBI AGGCCT 2 cut(s) 354, 420
SspI AATATT 1 cut(s) 835
SstI GAGCTC 1 cut(s) 1065
StuI AGGCCT 2 cut(s) 354, 420
StyD4I CCNGG 8 cut(s) 211, 502, 1070, 1193, 2469, 2517, 2719, 3091
TaiI ACGT 4 cut(s) 1493, 1568, 1707, 1833
TaqII GACCGA 2 cut(s) 2261, 2379
TatI WGTACW 1 cut(s) 1592
TauI GCSGC 2 cut(s) 2237, 2660
TseFI GTSAC 9 cut(s) 13, 491, 743, 886, 1076, 1124, 1381, 1463, 2397
Tsp45I GTSAC 9 cut(s) 13, 491, 743, 886, 1076, 1124, 1381, 1463, 2397
TspGWI ACGGA 5 cut(s) 303, 815, 1644, 1960, 2769
Tth111I GACNNNGTC 2 cut(s) 939, 1564
VneI GTGCAC 1 cut(s) 1979
VpaK11BI GGWCC 3 cut(s) 1190, 2206, 2947
VspI ATTAAT 2 cut(s) 365, 1505
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 7 cut(s) 903, 1028, 1615, 1670, 2186, 2508, 2570
XbaI TCTAGA 1 cut(s) 1721
XceI RCATGY 4 cut(s) 590, 864, 878, 1536
XhoI CTCGAG 1 cut(s) 1157
XmiI GTMKAC 1 cut(s) 1924
XmnI GAANNNNTTC 3 cut(s) 1284, 2771, 3300
Zsp2I ATGCAT 1 cut(s) 2875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.