Rh6BG217500

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009215
Physical Location & Seq
Reverse (-)
41003000 .. 41010413
7414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG217500.1

Sequence Viewer

Length: 2541 bp
ATGGCAGATCTTGATAGCTACGGGAATGCCAACCGTGACATTGACCAGGCAATAATTGCTTTGAAGAAGGGTGCTCAGTTACTGAAATATGGTCGTAAGGGAAAGCCTAAGTTTTGTCCATTTCGACTCTCTAGCGACGAATCATCTTTGATCTGGATTTCGAGTAGTGGAGAAAGAACTTTGAAGTTAGCTTCTGTCTCAAGAATTATTCCTGGGCAAAGAACTGCCGTTTTCCAACGATATCTGCGTCCTGAAAAGGATTATTTATCTTTTTCTCTTATATATAATAACGGAAAGCGTTCCCTTGATCTGATTTGCAAGGATAAAGTCGAGGCAGAAGTGTGGATTGCGGGCCTCAAAGCACTTATATCCTCTGGTAGAGGTGGCCGCTCCAAAATTGATGGATGGAGTGATGGAGGCTTGTACCTTGATGATAGCAGAGACTTGACATCAAATAGCCCTAGTGATAGTTCAGTTAGTGGTACACGAGATATTGGGTCTCCTGAGATTTCTGTCAGTTTCAAACCAAATACTTCACCGAAGAGTTTTCCGCCTGACAATTCTCCGGTCTCTGAGAGGTCACATGTAGCATCAGACCAGACAAATATGCAAGTAAAAGGATCCGGTTCAGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCCAGCACTTCAAGTCATGGTTCTGCACCCGATGATTGTGATGCTCTAGGTGATGTATACATATGGGGTGAGGTCATTTGTGATAGTGTTGTTAAGATTGGAGCTGATAAAAATGTAAATTATTTGAGTCCCCGAGCAGATGTGCTTGTTCCTAGGCCATTAGAGTCCAATGTTGTTTTGGATGTACATCATATAGCCTGTGGGGTCAAGCATGCAGCCTTGGTCACAAGACAAGGTGAAGTTTTTACATGGGGTGAAGAATCTGGAGGACGACTTGGCCATGGTGTGGGGAAGGATGTTGCTCAACCCCGTCTAGTTGAATCTCTGGCAGCTACTAGTGTTGATTTTGCGGCGTGTGGAGAATTTCATAGTTGTGCTGTTACAATGGCTGGGGAACTTTATACGTGGGGAGATGGTACACACAATGCTGGGCTTCTTGGTCATGGCACTGATGTCAGTCACTGGATACCAAAGAGAATTTCAGGTCCTCTTGAGGGACTTCAAGTTGCTTCTGTATCTTGTGGTCCATGGCATACGGCCTTGGTGACATCAACGGGGAAACTTTTTACATTTGGCGATGGAACATTTGGTGTCTTAGGCCATGGAGACAGAGAAAATGTTTCATATCCGAGAGAAGTAGATTCCCTAACAGGTTTGAGGACAATTGCTGTTGCATGTGGGGTATGGCACACTGCTGCTGTAGTAGAGGTTATTGCAACACAATCTAGTGCCAGTATTTCATCAGGTAAATTGTTTACTTGGGGTGATGGAGATAAAAATCGTCTTGGACATGGAGACAAGGAAGCCCGGCTTAAACCGACGTGTGTTCCAGCCCTTATTGATTACAATTTTCACAAAATTGCTTGCGGGCACAGTTTAACTGTTGGCTTGACAACATCAGGGCATGTCTTTACAATGGGTAGTACTGTTTATGGTCAGCTCGGAAATCCCAATTCTGATGGAAAAATACCTTGCTTAGTGGATGACAAACTTTCCGGGGAATGTATTGAAGAAATTGCTTGTGGTGCATATCATGTAGCAGTATTAACATCCAGAAATGAAGTTTATACATGGGGAAAAGGTGCTAATGGGAGGTTGGGGCATGGTGATATTGAGGATCGAAAAACACCGACTATGGTCGAAGGTTTGAAGGATAGACATGTGAAATATATTGGTTGTGGTTCACACTATACAGCGGCTATATGTCTTCACAAATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGATTTACAAGGAAGAGGCATAATTGCTATAATTGTGGACTTGTGCACTGCCATTCATGTAGTTCCAGAAAAGCAACAAGAGCAGCACTGTCTCCTAATCCTGGAAAACCGTATCGAGTGTGTGATTCTTGTTATGTGAAATTGAACAAGGTGTTAGAACCTGGTAGTAATAATAGAAAGAATGTTATACCTCGCTTGTCTGGTGAGAACAAGGACAGATTGGACAAGGCTGACATAAGATTATACAAGTCTGCTGTACCTTCAAACGTGGATTTGATAAAGCAATTAGATAGCAAAGCAGCCAAGCAAGGAAAGAAAGCTGAGACATTTTCTCTTGTTCGCTCCTCTCAAGCACCTTCGCTGTTGCAATTAAAGGATGTAGTTATGTCAGCTGCTGTTGATCTGAGACGAACAGTTCCCAAACCAGTTCTTACACCATCTGGAGTAAGTTCGAGGTCTGTGTCGCCTTTCTCAAGGAGACCAAGCCCTCCTCGTTTTGCAACTCCTGTTCCTACAACATCTGGACTTTCCTTCTCAAAAAGTATCGCAGATAGTTTGAAGAAAACTAATGAGCTTTTGAATCAGGAAGTGCTAAAGTTACGTTCACAGGTATAA

Protein Analysis

846

Amino Acids

90.89

Weight (kDa)

8.94

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 123 1.3e-08 Pleckstrin homology domain
WD40_RLD PF25390 239 - 620 2.9e-77 RCC1-like domain
RCC1 PF00415 298 - 347 5e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 351 - 402 3.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 389 - 417 1.2e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 406 - 454 1e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 468 - 516 5.9e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 520 - 569 4.4e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 557 - 584 4e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 574 - 621 7.3e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 625 - 691 3.3e-15 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 946
AccBSI CCGCTC 1 cut(s) 390
AccI GTMKAC 2 cut(s) 717, 1915
AciI CCGC 6 cut(s) 350, 388, 551, 1010, 1527, 1856
AclWI GGATC 3 cut(s) 615, 628, 1785
AcoI YGGCCR 2 cut(s) 385, 937
AcsI RAATTY 2 cut(s) 1022, 1137
AfaI GTAC 6 cut(s) 425, 484, 846, 1078, 1585, 2184
AfiI CCNNNNNNNGG 3 cut(s) 946, 1154, 2027
AflIII ACRYGT 3 cut(s) 583, 1481, 1819
AhlI ACTAGT 1 cut(s) 995
AjiI CACGTC 1 cut(s) 1482
AjnI CCWGG 4 cut(s) 45, 211, 2026, 2086
AjuI GAANNNNNNNTTGG 2 cut(s) 1123, 1155
AluBI AGCT 8 cut(s) 18, 191, 764, 992, 1600, 2246, 2318, 2500
AluI AGCT 8 cut(s) 18, 191, 764, 992, 1600, 2246, 2318, 2500
Alw21I GWGCWC 4 cut(s) 76, 661, 1907, 1974
Alw44I GTGCAC 1 cut(s) 1970
AlwI GGATC 3 cut(s) 615, 628, 1785
AlwNI CAGNNNCTG 3 cut(s) 82, 1122, 2321
Ama87I CYCGRG 1 cut(s) 792
AoxI GGCC 6 cut(s) 352, 385, 815, 937, 1197, 1258
ApaLI GTGCAC 1 cut(s) 1970
ApeKI GCWGC 6 cut(s) 875, 989, 1355, 2009, 2225, 2318
ApoI RAATTY 2 cut(s) 1022, 1137
Asp700I GAANNNNTTC 1 cut(s) 298
AspA2I CCTAGG 1 cut(s) 812
AspS9I GGNCC 3 cut(s) 352, 1145, 1184
AsuC2I CCSGG 2 cut(s) 1468, 1657
AsuHPI GGTGA 9 cut(s) 528, 722, 740, 908, 926, 1216, 1436, 1778, 2141
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 2 cut(s) 1145, 1184
AvrII CCTAGG 1 cut(s) 812
BaeGI GKGCMC 2 cut(s) 1533, 1974
BalI TGGCCA 1 cut(s) 939
BamHI GGATCC 1 cut(s) 620
BauI CACGAG 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 1859
Bbv12I GWGCWC 4 cut(s) 76, 661, 1907, 1974
BbvI GCAGC 6 cut(s) 887, 1001, 1342, 2021, 2237, 2305
BccI CCATC 8 cut(s) 395, 399, 407, 1067, 1232, 1421, 1613, 2371
BceAI ACGGC 2 cut(s) 212, 1212
BciT130I CCWGG 4 cut(s) 47, 213, 2028, 2088
BciVI GTATCC 1 cut(s) 1119
BcnI CCSGG 2 cut(s) 1468, 1657
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 7 cut(s) 132, 462, 707, 813, 974, 996, 1386
BfmI CTRYAG 1 cut(s) 1359
BfuI GTATCC 1 cut(s) 1119
BglII AGATCT 1 cut(s) 7
BisI GCNGC 9 cut(s) 388, 876, 990, 1011, 1356, 1857, 2010, 2226, 2319
BlnI CCTAGG 1 cut(s) 812
BlpI GCTNAGC 1 cut(s) 1890
BlsI GCNGC 9 cut(s) 389, 877, 991, 1012, 1357, 1858, 2011, 2227, 2320
BmcAI AGTACT 1 cut(s) 1585
Bme1390I CCNGG 6 cut(s) 47, 213, 1468, 1657, 2028, 2088
Bme18I GGWCC 2 cut(s) 1145, 1184
BmeT110I CYCGRG 1 cut(s) 792
BmgBI CACGTC 1 cut(s) 1482
BmgT120I GGNCC 3 cut(s) 352, 1145, 1184
BmiI GGNNCC 1 cut(s) 622
BmrFI CCNGG 6 cut(s) 47, 213, 1468, 1657, 2028, 2088
BmsI GCATC 3 cut(s) 599, 622, 691
BoxI GACNNNNGTC 1 cut(s) 1796
BpiI GAAGAC 1 cut(s) 1859
BpmI CTGGAG 2 cut(s) 945, 2388
Bpu1102I GCTNAGC 1 cut(s) 1890
BpuEI CTTGAG 4 cut(s) 184, 1172, 2259, 2383
BpuMI CCSGG 2 cut(s) 1468, 1657
BsaAI YACGTR 1 cut(s) 1065
BsaBI GATNNNNATC 2 cut(s) 846, 1437
BsaI GGTCTC 3 cut(s) 504, 574, 2398
BsaJI CCNNGG 8 cut(s) 212, 812, 879, 940, 1187, 1200, 1261, 1656
BsaWI WCCGGW 2 cut(s) 565, 623
Bsc4I CCNNNNNNNGG 3 cut(s) 946, 1154, 2027
Bse1I ACTGG 3 cut(s) 1127, 1392, 2351
Bse8I GATNNNNATC 2 cut(s) 846, 1437
BseBI CCWGG 4 cut(s) 47, 213, 2028, 2088
BseDI CCNNGG 8 cut(s) 212, 812, 879, 940, 1187, 1200, 1261, 1656
BseGI GGATG 6 cut(s) 410, 847, 961, 1648, 1709, 2308
BseJI GATNNNNATC 2 cut(s) 846, 1437
BseLI CCNNNNNNNGG 3 cut(s) 946, 1154, 2027
BseMII CTCAG 6 cut(s) 89, 495, 564, 1881, 2238, 2321
BseNI ACTGG 3 cut(s) 1127, 1392, 2351
BseRI GAGGAG 2 cut(s) 2260, 2406
BseSI GKGCMC 2 cut(s) 1533, 1974
BseXI GCAGC 6 cut(s) 887, 1001, 1342, 2021, 2237, 2305
BseYI CCCAGC 3 cut(s) 662, 1049, 1088
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 6 cut(s) 354, 387, 817, 939, 1199, 1260
BsiHKAI GWGCWC 4 cut(s) 76, 661, 1907, 1974
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 4 cut(s) 566, 624, 1468, 1656
BslFI GGGAC 2 cut(s) 774, 1170
BslI CCNNNNNNNGG 3 cut(s) 946, 1154, 2027
BsmBI CGTCTC 1 cut(s) 2326
BsmFI GGGAC 2 cut(s) 774, 1170
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 6 cut(s) 354, 387, 817, 939, 1199, 1260
Bso31I GGTCTC 3 cut(s) 504, 574, 2398
BsoBI CYCGRG 1 cut(s) 792
Bsp1286I GDGCHC 5 cut(s) 76, 661, 1533, 1907, 1974
Bsp1407I TGTACA 1 cut(s) 844
Bsp143I GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
Bsp1720I GCTNAGC 1 cut(s) 1890
Bsp19I CCATGG 3 cut(s) 940, 1187, 1261
BspACI CCGC 6 cut(s) 350, 388, 551, 1010, 1527, 1856
BspANI GGCC 6 cut(s) 354, 387, 817, 939, 1199, 1260
BspCNI CTCAG 6 cut(s) 88, 496, 565, 1882, 2239, 2322
BspLI GGNNCC 1 cut(s) 622
BspPI GGATC 3 cut(s) 615, 628, 1785
BspTNI GGTCTC 3 cut(s) 504, 574, 2398
BsrBI CCGCTC 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 844
BsrI ACTGG 3 cut(s) 1127, 1392, 2351
BssECI CCNNGG 8 cut(s) 212, 812, 879, 940, 1187, 1200, 1261, 1656
BssMI GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
BssNAI GTATAC 1 cut(s) 718
BssSI CACGAG 1 cut(s) 486
BssT1I CCWWGG 6 cut(s) 812, 879, 940, 1187, 1200, 1261
Bst1107I GTATAC 1 cut(s) 718
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 4 cut(s) 47, 213, 2028, 2088
Bst4CI ACNGT 8 cut(s) 35, 1535, 1543, 1588, 1902, 2016, 2037, 2341
Bst6I CTCTTC 2 cut(s) 536, 1934
BstAPI GCANNNNNTGC 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 844
BstBAI YACGTR 1 cut(s) 1065
BstC8I GCNNGC 4 cut(s) 352, 873, 1525, 1529
BstDEI CTNAG 9 cut(s) 75, 108, 504, 573, 1255, 1636, 1890, 2247, 2330
BstDSI CCRYGG 3 cut(s) 940, 1187, 1261
BstF5I GGATG 6 cut(s) 410, 847, 961, 1648, 1709, 2308
BstKTI GATC 6 cut(s) 10, 153, 310, 623, 1780, 2329
BstMBI GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
BstMWI GCNNNNNNNGC 3 cut(s) 56, 1685, 2006
BstNI CCWGG 4 cut(s) 47, 213, 2028, 2088
BstNSI RCATGY 5 cut(s) 587, 875, 1338, 1568, 1823
BstPAI GACNNNNGTC 1 cut(s) 1796
BstSCI CCNGG 6 cut(s) 45, 211, 1466, 1655, 2026, 2086
BstSFI CTRYAG 1 cut(s) 1359
BstSLI GKGCMC 2 cut(s) 1533, 1974
BstV1I GCAGC 6 cut(s) 887, 1001, 1342, 2021, 2237, 2305
BstV2I GAAGAC 1 cut(s) 1859
BstX2I RGATCY 2 cut(s) 7, 620
BstYI RGATCY 2 cut(s) 7, 620
BstZ17I GTATAC 1 cut(s) 718
BsuI GTATCC 1 cut(s) 1119
BsuRI GGCC 6 cut(s) 354, 387, 817, 939, 1199, 1260
BtgI CCRYGG 3 cut(s) 940, 1187, 1261
BtgZI GCGATG 1 cut(s) 1251
BtrI CACGTC 1 cut(s) 1482
BtsCI GGATG 6 cut(s) 410, 847, 961, 1648, 1709, 2308
BtsI GCAGTG 2 cut(s) 1350, 1972
BtsIMutI CAGTG 6 cut(s) 1107, 1120, 1350, 1907, 1972, 2012
Cac8I GCNNGC 4 cut(s) 352, 873, 1525, 1529
CaiI CAGNNNCTG 3 cut(s) 82, 1122, 2321
Cfr13I GGNCC 3 cut(s) 352, 1145, 1184
CseI GACGC 1 cut(s) 236
CsiI ACCWGGT 1 cut(s) 2086
Csp6I GTAC 6 cut(s) 424, 483, 845, 1077, 1584, 2183
CviQI GTAC 6 cut(s) 424, 483, 845, 1077, 1584, 2183
DdeI CTNAG 9 cut(s) 75, 108, 504, 573, 1255, 1636, 1890, 2247, 2330
DpnI GATC 6 cut(s) 9, 152, 309, 622, 1779, 2328
DpnII GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
EaeI YGGCCR 2 cut(s) 385, 937
Eam1104I CTCTTC 2 cut(s) 536, 1934
EarI CTCTTC 2 cut(s) 536, 1934
EciI GGCGGA 1 cut(s) 540
Eco130I CCWWGG 6 cut(s) 812, 879, 940, 1187, 1200, 1261
Eco31I GGTCTC 3 cut(s) 504, 574, 2398
Eco32I GATATC 1 cut(s) 242
Eco47I GGWCC 2 cut(s) 1145, 1184
Eco88I CYCGRG 1 cut(s) 792
EcoO109I RGGNCCY 1 cut(s) 1145
EcoRII CCWGG 4 cut(s) 45, 211, 2026, 2086
EcoRV GATATC 1 cut(s) 242
EcoT14I CCWWGG 6 cut(s) 812, 879, 940, 1187, 1200, 1261
EcoT22I ATGCAT 1 cut(s) 637
ErhI CCWWGG 6 cut(s) 812, 879, 940, 1187, 1200, 1261
Esp3I CGTCTC 1 cut(s) 2326
FalI AAGNNNNNCTT 2 cut(s) 1455, 1487
FaqI GGGAC 2 cut(s) 774, 1170
FauI CCCGC 2 cut(s) 343, 1520
FauNDI CATATG 1 cut(s) 722
FblI GTMKAC 2 cut(s) 717, 1915
Fnu4HI GCNGC 9 cut(s) 388, 876, 990, 1011, 1356, 1857, 2010, 2226, 2319
FokI GGATG 6 cut(s) 417, 854, 968, 1655, 1696, 2315
Fsp4HI GCNGC 9 cut(s) 388, 876, 990, 1011, 1356, 1857, 2010, 2226, 2319
FspBI CTAG 7 cut(s) 132, 462, 707, 813, 974, 996, 1386
GluI GCNGC 9 cut(s) 388, 876, 990, 1011, 1356, 1857, 2010, 2226, 2319
GsaI CCCAGC 3 cut(s) 666, 1053, 1092
GsuI CTGGAG 2 cut(s) 945, 2388
HaeIII GGCC 6 cut(s) 354, 387, 817, 939, 1199, 1260
HapII CCGG 4 cut(s) 566, 624, 1468, 1656
HgaI GACGC 1 cut(s) 236
HinfI GANTC 9 cut(s) 126, 140, 787, 824, 920, 980, 1301, 2051, 2506
HpaII CCGG 4 cut(s) 566, 624, 1468, 1656
HphI GGTGA 9 cut(s) 528, 722, 740, 908, 926, 1216, 1436, 1778, 2141
Hpy166II GTNNAC 9 cut(s) 485, 718, 1079, 1416, 1844, 1916, 1964, 1972, 2531
Hpy188I TCNGA 8 cut(s) 312, 574, 595, 631, 1290, 1604, 1618, 2331
Hpy8I GTNNAC 9 cut(s) 485, 718, 1079, 1416, 1844, 1916, 1964, 1972, 2531
Hpy99I CGWCG 2 cut(s) 140, 1483
HpyAV CCTTC 7 cut(s) 61, 946, 1796, 1804, 2196, 2292, 2467
HpyCH4III ACNGT 8 cut(s) 35, 1535, 1543, 1588, 1902, 2016, 2037, 2341
HpyCH4IV ACGT 4 cut(s) 1064, 1481, 2193, 2527
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 1685, 2006
HpyF3I CTNAG 9 cut(s) 75, 108, 504, 573, 1255, 1636, 1890, 2247, 2330
HpySE526I ACGT 4 cut(s) 1064, 1481, 2193, 2527
Kzo9I GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
LmnI GCTCC 4 cut(s) 395, 664, 761, 2273
Lsp1109I GCAGC 6 cut(s) 887, 1001, 1342, 2021, 2237, 2305
LweI GCATC 3 cut(s) 599, 622, 691
MabI ACCWGGT 1 cut(s) 2086
MaeI CTAG 7 cut(s) 132, 462, 707, 813, 974, 996, 1386
MaeII ACGT 4 cut(s) 1064, 1481, 2193, 2527
MaeIII GTNAC 9 cut(s) 35, 78, 579, 883, 1039, 1118, 1204, 1896, 2523
MalI GATC 6 cut(s) 9, 152, 309, 622, 1779, 2328
MbiI CCGCTC 1 cut(s) 390
MboI GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
MboII GAAGA 7 cut(s) 76, 553, 929, 1682, 1859, 1951, 2497
MfeI CAATTG 1 cut(s) 1323
MflI RGATCY 2 cut(s) 7, 620
MhlI GDGCHC 5 cut(s) 76, 661, 1533, 1907, 1974
MlsI TGGCCA 1 cut(s) 939
MluNI TGGCCA 1 cut(s) 939
MlyI GAGTC 3 cut(s) 120, 796, 833
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 939
Mph1103I ATGCAT 1 cut(s) 637
MroXI GAANNNNTTC 1 cut(s) 298
MscI TGGCCA 1 cut(s) 939
MseI TTAA 5 cut(s) 753, 1473, 1538, 1706, 2297
MslI CAYNNNNRTG 1 cut(s) 1032
Msp20I TGGCCA 1 cut(s) 939
MspA1I CMGCKG 2 cut(s) 1856, 2318
MspI CCGG 4 cut(s) 566, 624, 1468, 1656
MspR9I CCNGG 6 cut(s) 47, 213, 1468, 1657, 2028, 2088
MunI CAATTG 1 cut(s) 1323
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 4 cut(s) 47, 213, 2028, 2088
MwoI GCNNNNNNNGC 3 cut(s) 56, 1685, 2006
NciI CCSGG 2 cut(s) 1468, 1657
NcoI CCATGG 3 cut(s) 940, 1187, 1261
NdeI CATATG 1 cut(s) 722
NdeII GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
NlaIV GGNNCC 1 cut(s) 622
NmuCI GTSAC 6 cut(s) 35, 579, 883, 1118, 1204, 1896
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 5 cut(s) 587, 875, 1338, 1568, 1823
PaeI GCATGC 1 cut(s) 875
PciI ACATGT 2 cut(s) 583, 1819
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 298
PfeI GAWTC 6 cut(s) 140, 920, 980, 1301, 2051, 2506
PflMI CCANNNNNTGG 1 cut(s) 946
PfoI TCCNGGA 1 cut(s) 2026
PkrI GCNGC 9 cut(s) 389, 877, 991, 1012, 1357, 1858, 2011, 2227, 2320
PleI GAGTC 3 cut(s) 120, 795, 832
PpsI GAGTC 3 cut(s) 120, 795, 832
Ppu21I YACGTR 1 cut(s) 1065
PpuMI RGGWCCY 1 cut(s) 1145
PscI ACATGT 2 cut(s) 583, 1819
PshAI GACNNNNGTC 1 cut(s) 1796
Psp5II RGGWCCY 1 cut(s) 1145
Psp6I CCWGG 4 cut(s) 45, 211, 2026, 2086
PspFI CCCAGC 3 cut(s) 662, 1049, 1088
PspGI CCWGG 4 cut(s) 45, 211, 2026, 2086
PspN4I GGNNCC 1 cut(s) 622
PspPI GGNCC 3 cut(s) 352, 1145, 1184
PspPPI RGGWCCY 1 cut(s) 1145
PstNI CAGNNNCTG 3 cut(s) 82, 1122, 2321
PsuI RGATCY 2 cut(s) 7, 620
PvuII CAGCTG 1 cut(s) 2318
RsaI GTAC 6 cut(s) 425, 484, 846, 1078, 1585, 2184
RsaNI GTAC 6 cut(s) 424, 483, 845, 1077, 1584, 2183
RseI CAYNNNNRTG 1 cut(s) 1032
SaqAI TTAA 5 cut(s) 753, 1473, 1538, 1706, 2297
SatI GCNGC 9 cut(s) 388, 876, 990, 1011, 1356, 1857, 2010, 2226, 2319
Sau3AI GATC 6 cut(s) 7, 150, 307, 620, 1777, 2326
Sau96I GGNCC 3 cut(s) 352, 1145, 1184
ScaI AGTACT 1 cut(s) 1585
SchI GAGTC 3 cut(s) 120, 796, 833
ScrFI CCNGG 6 cut(s) 47, 213, 1468, 1657, 2028, 2088
SduI GDGCHC 5 cut(s) 76, 661, 1533, 1907, 1974
SexAI ACCWGGT 1 cut(s) 2086
SfaNI GCATC 3 cut(s) 599, 622, 691
SfcI CTRYAG 1 cut(s) 1359
SinI GGWCC 2 cut(s) 1145, 1184
SmiMI CAYNNNNRTG 1 cut(s) 1032
SmlI CTYRAG 4 cut(s) 199, 1151, 2274, 2398
SmoI CTYRAG 4 cut(s) 199, 1151, 2274, 2398
SpeI ACTAGT 1 cut(s) 995
SphI GCATGC 1 cut(s) 875
SsiI CCGC 6 cut(s) 350, 388, 551, 1010, 1527, 1856
SspMI CTAG 7 cut(s) 132, 462, 707, 813, 974, 996, 1386
StyD4I CCNGG 6 cut(s) 45, 211, 1466, 1655, 2026, 2086
StyI CCWWGG 6 cut(s) 812, 879, 940, 1187, 1200, 1261
TaaI ACNGT 8 cut(s) 35, 1535, 1543, 1588, 1902, 2016, 2037, 2341
TaiI ACGT 4 cut(s) 1067, 1484, 2196, 2530
TaqI TCGA 8 cut(s) 124, 161, 330, 640, 1780, 1800, 2041, 2378
TatI WGTACW 2 cut(s) 844, 1583
TauI GCSGC 3 cut(s) 390, 1013, 1859
TfiI GAWTC 6 cut(s) 140, 920, 980, 1301, 2051, 2506
Tru1I TTAA 5 cut(s) 753, 1473, 1538, 1706, 2297
Tru9I TTAA 5 cut(s) 753, 1473, 1538, 1706, 2297
TscAI CASTG 6 cut(s) 1114, 1127, 1357, 1907, 1979, 2019
TseFI GTSAC 6 cut(s) 35, 579, 883, 1118, 1204, 1896
TseI GCWGC 6 cut(s) 875, 989, 1355, 2009, 2225, 2318
Tsp45I GTSAC 6 cut(s) 35, 579, 883, 1118, 1204, 1896
TspDTI ATGAA 5 cut(s) 1016, 1272, 1389, 1734, 1971
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 6 cut(s) 1114, 1127, 1357, 1907, 1979, 2019
Van91I CCANNNNNTGG 1 cut(s) 946
VneI GTGCAC 1 cut(s) 1970
VpaK11BI GGWCC 2 cut(s) 1145, 1184
XapI RAATTY 2 cut(s) 1022, 1137
XceI RCATGY 5 cut(s) 587, 875, 1338, 1568, 1823
XcmI CCANNNNNNNNNTGG 1 cut(s) 835
XmaJI CCTAGG 1 cut(s) 812
XmiI GTMKAC 2 cut(s) 717, 1915
XmnI GAANNNNTTC 1 cut(s) 298
XspI CTAG 7 cut(s) 132, 462, 707, 813, 974, 996, 1386
ZrmI AGTACT 1 cut(s) 1585
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.