Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009215

Physical Location & Seq

Genomic Coordinates Forward (+)

3467555 .. 3476140

Gene Structure 8586 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1020200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3393 bp

ATGGCAGATCTTGTTAGCTACGGGAATGCCAACCGTGACATCGACCAGGCAATAATTGCTTTGAAGAAGGGTGCTCAACTACTGAAATATGGTCGTAAGGGAAAGCCTAAGTTTTGTCCATTTAGACTTTCTACTGATGAATCAACTTTGATCTGGATTTCAAGTAGCGAAAGAAGTTTGAAGTTGGCTTCTGTCACAAGAATTGTTCCTGGACAAAGAACTGCTGTCTTTCAACGATATTTACGTCCTGAAAAGGACTATTTATCATTTTCTCTTATATACAATAACGGAAAGCGGTCTCTTGATCTGATTTGCAAGGACAAAGTTGAGGCAGAAGTGTGGATTGCAGGCCTTAAAGCACTACTTTCCTCTGGCAGAGGTGGGCGTTCAAAAATTGATGGATGGAGTGATGGAGGCCTCTACCTTGATGATGGCAAGGACTTGACATCAAATAGTCCTAGTGATAGTTCTGCTAGTGGTGCACGAGATAGTGGCTCTCCAGAGATTTCTGTTAATTTCAAACCAAATACTTCGCCTAAGAGTTTTCCCCCTGAAAATTCTCCAGTTTCTGAGAGGTCACATGCTGCATCAGACCTGACAAATATGCAAGTTAAAGGATCTGGTTCAGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCTAGCACTTCAAGTCATGGTTCTGGACCGGATGATTGTGAAGCTCTAGGTGATGTGTACATATGGGGTGAGGTTATTTGTGATAGTGTTGTTAAGGTTGGGGCTGATAAAAATGTGAATTATTTGAGCCCGAGATCAGATGTGCTTGTCCCTAGGCCATTAGAGTCCAATGTTGTTTTGGATGTCCATCATATAGCCTGTGGGGTCAAGCATGCAGCCTTGGTCACGAGACAAGGTGAAGTGTTTACATGGGGTGAAGAATCTGGAGGACGACTAGGGCATGGTGCTGGGAAGGATGTTGTTCAACCTCATCTAGTTGAATCTCTGGCAGCTACTAGTGTTGATTTTGCAGCATGTGGAGAATTTCATACATGTGCTGTTACAATGGCTGGGGAACTTTATACGTGGGGAGATGGTACGCATAATGCTGGGCTACTTGGTCATGGCACTGATGTCAGCCATTGGATACCAAAGAGAATTTCGGGTCCTCTTGAGGGACTTCAAGTTGCTTCTGTAACTTGTGGTCCATGGCATACAGCCTTGATAACATCAACGGGGAAACTCTTCACATTCGGTGATGGCACATTTGGCGTCTTAGGCCATGGAGACAGAGAAAATGTTCCTTATCCGAGAGAAGTAGAATCCCTATCAGGGTTGAGGACAATATCTGTTGCATGTGGGGTGTGGCACACTGCTGCTGTGGTGGAGGTTATTGCAACACAATCTAGTGCAAGTATTTCGTCAGGTAAGTTGTTTACTTGGGGTGATGGAGATAAAAATCGTCTCGGGCATGGGGACAAGGAAGCCCGACTTAAACCGACTTGTGTTCCAGCCCTTATTGACTACAGTTTTCACAAAATTGCTTGCGGGCACAGTTTAACAGTTGGCTTGACGACTTCAGGACATGTTTTTACAATGGGCAGTACTGTTTATGGTCAGCTTGGGAATCCCAATTCTGATGGAAAGCTACCTTGCTTGGTAGAAGACAAACTCGGTGGGGATTGTATTGAAGAAATTGCATGTGGTGCTTATCATGTAGCAGTATTAACATCCAGGAATGAAGTTTATACATGGGGAAAGGGAGCTAATGGGAGATTAGGCCATGGAGATGTTGAAGATCGGAAAACACCGACTTTGGTTGAAGCTTTAAAGGATAGACATGTGAAATATATTTGTTGCGGTTCAGAATATACAGCAGCTATTTGTCTTCACAAATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGGTTCACAAGGAAGAGGCACAACTGCTATAATTGTGGACTTGTGCACTGCCACTCATGCAGTTCAAAAAAAGCAACAAGGGCAGCTCTGGCTCCAAATCCTGGAAAACCATATCGAGTTTGTGATCCTTGTTATGTGAAATTGAATAAGGTGTCAGAAACTGGTAGTAATAACAGAAGGAATGCTATACCTCGGTTGTCGGGTGAGAACAAGGACAGGTTGGACAAGGCTGACATAAGATTATACAAGACTGCTGTACCATCGAATATGGATTTGATAAAGCAATTAGATACTAAAGCAGCCAAGCAGGGAAAGAAAGCTGACACATTTTCACTTGTCCGCTCTGCTCAAGCACCTTCATTGTTGCAATTAAAGGAGGTTGTTATGTCAACGGCTGTTGATCTGCGACGAACAGTTCCCAAACCAGTTCTTACACCATCTGGAGCAAGTTCAAGGTCCGTGTCCCCTTTCTCGAGGAGACCAAGCCCCCCTCGTTTTGCAACTCCTGTTCCTACTACATCAGGACTTTCCTTCTCAAAAAGTATTGCTGATAGTCTGAAGAAAACAAATGAACTTTTGAATCAGGAGGTGCTAAAATTACGCTCACGGGTTGAGAGCCTTAGACAGAGATGTGATCGTCAAGAATTAGAGCTTCAGAATTCTTCAAAGAAAGTACAAGAAGCAATGGCATTGGCCGCAGACGAATCTGCTAAATCTAAAGCTGCAAAAGAAGTTATAAAGTCACTAACCGCACAGCTCAAGGATTTGGCGGAACGGCTTTCACCTGGGGTTTATGATACAGAGACCATAAAGCAGGCATTACTGCCAAATGGTTTGGAGCCAAATGGCATTAACTATCCAGATGCTAATGAAGAGCAGCATTCAAGATCGACTTCCATCAGCAGTTCTTACTTGATCTCCTCCTTGGGAATCGACTCTGCTACAACAAATGGAAATCACGGGCCGACTCTTTCACCCAAGGGTCAACTTGGAACAAACGAAACAATCGTGCAGCACAGTCGGGGCCCTCTCACCCCCAATGGGATGATAAACTCCCCAGATAAACTACCCAATGGTGGTGGATCATTTGAGACAGTTGGTAGTAGTGCGTCTGAAACTGTTGATGGCAGGGAATCCGGGCCTTTTCGAGATGGGGAGAATGGTGCGAGATCCATGAATTCTCCATCGCCTGCTAATGGTAATACAGTTGAGGCAGAATGGATTGAACAATATGAGCCTGGTGTTTATATAACTCTTGTGGCTCTCCGGGATGGAACTAGAGATCTCAAACGAGTGCGCTTCAGCCGAAGGAGATTTGGAGAGCACCAAGCACAGATCTGGTGGTCGGAGAATCGTGAGAAAGTGTATGAGAAGTACAATGTGCGTGGGTCAGACAGATCTTCGGTTGCTGGCTCAGCTGCACGTAGATCAGACGGAGCTCTCTCACCGGCCTCATCTCAACAGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005085 GO:0005086 GO:0005088 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005829 GO:0008150 GO:0008289 GO:0012505 GO:0016020 GO:0017016 GO:0017112 GO:0017137 GO:0019899 GO:0031267 GO:0035091 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051020 GO:0065007 GO:0065009 GO:0070300 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1131

Amino Acids

121.57

Weight (kDa)

8.73

Isoelectric Point (pI)

39.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_12	PF16457	16 - 122	2e-09	Pleckstrin homology domain
WD40_RLD	PF25390	251 - 619	2.7e-76	RCC1-like domain
RCC1	PF00415	297 - 346	1.5e-09	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	350 - 401	1.3e-09	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	388 - 416	7.2e-06	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	405 - 453	1.4e-14	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	467 - 515	1.3e-11	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	519 - 568	3.5e-08	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	556 - 583	5.5e-07	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	573 - 620	9.8e-15	Regulator of chromosome condensation (RCC1) repeat
FYVE	PF01363	624 - 690	3e-15	FYVE zinc finger
BRX_N	PF13713	875 - 910	1.9e-17	Transcription factor BRX N-terminal domain
BRX	PF08381	1045 - 1100	7.4e-29	Transcription factor regulating root and shoot growth via Pin3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76950 AT5G42140 AT5G42140
fragaria_vesca	FvH4_2g14280 FvH4_2g14280 FvH4_2g14280 FvH4_2g14280
malus_domestica	MD05G1020200.v1.1 MD10G1021300.v1.1
prunus_persica	Prupe.8G024300_v2.0.a1
pyrus_communis	pycom05g01180 pycom10g01440
rosa_chinensis	RchiOBHm_Chr6g0276571
rosa_laevigata	RLG00000013389
rosa_multiflora	Rmu_ssc0000059.1_g000010
rosa_roxburghii	Rroxscaffold_7G00192170
rosa_rugosa	Rorug06G0101800
rosa_samantha	Rh6AG212600 Rh6BG217500 Rh6CG219800 Rh6DG209400
rosa_wichuraiana	Rw6G018580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2660
AccB7I	CCANNNNNTGG	1 cut(s)	2024
AccBSI	CCGCTC	1 cut(s)	2265
AccI	GTMKAC	1 cut(s)	1912
AciI	CCGC	7 cut(s)	295, 1524, 1836, 2263, 2619, 2673, 2693
AclWI	GGATC	4 cut(s)	625, 2042, 3013, 3087
AcoI	YGGCCR	1 cut(s)	2616
AcsI	RAATTY	5 cut(s)	556, 1019, 1134, 2581, 3100
AcuI	CTGAAG	4 cut(s)	1539, 2501, 2561, 3208
AcyI	GRCGYC	1 cut(s)	1248
AfaI	GTAC	6 cut(s)	716, 1075, 1582, 2181, 2598, 3299
AfiI	CCNNNNNNNGG	6 cut(s)	1151, 1308, 2024, 2964, 2999, 3119
AflIII	ACRYGT	3 cut(s)	1028, 1561, 1816
AhlI	ACTAGT	1 cut(s)	992
AjnI	CCWGG	6 cut(s)	45, 208, 1709, 2023, 2707, 3160
AjuI	GAANNNNNNNTTGG	2 cut(s)	1120, 1152
Alw21I	GWGCWC	7 cut(s)	76, 484, 658, 1904, 1971, 3249, 3364
Alw26I	GTCTC	7 cut(s)	303, 880, 1257, 1445, 2395, 2720, 3008
Alw44I	GTGCAC	2 cut(s)	480, 1967
AlwI	GGATC	4 cut(s)	625, 2042, 3013, 3087
AlwNI	CAGNNNCTG	2 cut(s)	569, 2084
Ama87I	CYCGRG	3 cut(s)	787, 1442, 2395
ApaI	GGGCCC	1 cut(s)	2951
ApaLI	GTGCAC	2 cut(s)	480, 1967
ApoI	RAATTY	5 cut(s)	556, 1019, 1134, 2581, 3100
Asp700I	GAANNNNTTC	2 cut(s)	1220, 1275
AspA2I	CCTAGG	1 cut(s)	809
AspLEI	GCGC	1 cut(s)	3222
AspS9I	GGNCC	8 cut(s)	683, 1142, 1181, 2379, 2885, 2947, 2948, 3062
AsuC2I	CCSGG	2 cut(s)	3061, 3191
AvaI	CYCGRG	3 cut(s)	787, 1442, 2395
AvaII	GGWCC	4 cut(s)	683, 1142, 1181, 2379
AvrII	CCTAGG	1 cut(s)	809
BaeGI	GKGCMC	4 cut(s)	484, 1530, 1971, 2951
BaeI	ACNNNNGTAYC	2 cut(s)	2163, 2196
BanII	GRGCYC	3 cut(s)	788, 2951, 3364
BauI	CACGAG	2 cut(s)	483, 883
BbsI	GAAGAC	2 cut(s)	1647, 1856
Bbv12I	GWGCWC	7 cut(s)	76, 484, 658, 1904, 1971, 3249, 3364
BceAI	ACGGC	2 cut(s)	2331, 2714
BcgI	CGANNNNNNTGC	4 cut(s)	1377, 1411, 2924, 2958
BciT130I	CCWGG	6 cut(s)	47, 210, 1711, 2025, 2709, 3162
BciVI	GTATCC	1 cut(s)	1116
BcnI	CCSGG	2 cut(s)	3061, 3191
BcoDI	GTCTC	7 cut(s)	303, 880, 1257, 1445, 2395, 2720, 3008
BcuI	ACTAGT	1 cut(s)	992
BfmI	CTRYAG	1 cut(s)	1501
BfuI	GTATCC	1 cut(s)	1116
BglII	AGATCT	4 cut(s)	7, 3205, 3258, 3320
BlnI	CCTAGG	1 cut(s)	809
BlpI	GCTNAGC	2 cut(s)	1887, 3337
BmcAI	AGTACT	1 cut(s)	1582
Bme1390I	CCNGG	8 cut(s)	47, 210, 1711, 2025, 2709, 3061, 3162, 3191
Bme18I	GGWCC	4 cut(s)	683, 1142, 1181, 2379
BmeT110I	CYCGRG	3 cut(s)	787, 1442, 2395
BmgT120I	GGNCC	8 cut(s)	683, 1142, 1181, 2379, 2885, 2947, 2948, 3062
BmiI	GGNNCC	5 cut(s)	1143, 2016, 2763, 2948, 2949
BmrFI	CCNGG	8 cut(s)	47, 210, 1711, 2025, 2709, 3061, 3162, 3191
BmsI	GCATC	3 cut(s)	596, 619, 2776
BpiI	GAAGAC	2 cut(s)	1647, 1856
BpmI	CTGGAG	4 cut(s)	483, 546, 942, 2385
Bpu1102I	GCTNAGC	2 cut(s)	1887, 3337
BpuEI	CTTGAG	3 cut(s)	1169, 2256, 2666
BpuMI	CCSGG	2 cut(s)	3061, 3191
BsaAI	YACGTR	2 cut(s)	1062, 3347
BsaBI	GATNNNNATC	2 cut(s)	843, 1434
BsaHI	GRCGYC	1 cut(s)	1248
BsaI	GGTCTC	3 cut(s)	303, 2395, 2720
BsaJI	CCNNGG	9 cut(s)	809, 876, 1184, 1258, 1759, 2114, 2708, 2847, 2901
BsaWI	WCCGGW	1 cut(s)	685
BsaXI	ACNNNNNCTCC	2 cut(s)	2825, 2855
Bsc4I	CCNNNNNNNGG	6 cut(s)	1151, 1308, 2024, 2964, 2999, 3119
Bse118I	RCCGGY	1 cut(s)	3370
Bse1I	ACTGG	3 cut(s)	563, 2089, 2348
Bse3DI	GCAATG	1 cut(s)	2613
Bse8I	GATNNNNATC	2 cut(s)	843, 1434
BseBI	CCWGG	6 cut(s)	47, 210, 1711, 2025, 2709, 3162
BseDI	CCNNGG	9 cut(s)	809, 876, 1184, 1258, 1759, 2114, 2708, 2847, 2901
BseGI	GGATG	7 cut(s)	407, 694, 844, 958, 1706, 2973, 3199
BseJI	GATNNNNATC	2 cut(s)	843, 1434
BseLI	CCNNNNNNNGG	6 cut(s)	1151, 1308, 2024, 2964, 2999, 3119
BseMI	GCAATG	1 cut(s)	2613
BseMII	CTCAG	3 cut(s)	561, 1878, 3351
BseNI	ACTGG	3 cut(s)	563, 2089, 2348
BseRI	GAGGAG	2 cut(s)	2413, 2833
BseSI	GKGCMC	4 cut(s)	484, 1530, 1971, 2951
BseYI	CCCAGC	3 cut(s)	944, 1046, 1085
BsgI	GTGCAG	2 cut(s)	2954, 3327
BsiHKAI	GWGCWC	7 cut(s)	76, 484, 658, 1904, 1971, 3249, 3364
BsiHKCI	CYCGRG	3 cut(s)	787, 1442, 2395
BsiSI	CCGG	4 cut(s)	686, 3060, 3190, 3371
BslFI	GGGAC	4 cut(s)	791, 1167, 1466, 2371
BslI	CCNNNNNNNGG	6 cut(s)	1151, 1308, 2024, 2964, 2999, 3119
BsmAI	GTCTC	7 cut(s)	303, 880, 1257, 1445, 2395, 2720, 3008
BsmBI	CGTCTC	1 cut(s)	1445
BsmFI	GGGAC	4 cut(s)	791, 1167, 1466, 2371
BsmI	GAATGC	3 cut(s)	31, 2110, 2803
Bso31I	GGTCTC	3 cut(s)	303, 2395, 2720
BsoBI	CYCGRG	3 cut(s)	787, 1442, 2395
Bsp120I	GGGCCC	1 cut(s)	2947
Bsp1407I	TGTACA	1 cut(s)	714
Bsp1720I	GCTNAGC	2 cut(s)	1887, 3337
Bsp19I	CCATGG	3 cut(s)	1184, 1258, 1759
BspACI	CCGC	7 cut(s)	295, 1524, 1836, 2263, 2619, 2673, 2693
BspCNI	CTCAG	3 cut(s)	562, 1879, 3350
BspLI	GGNNCC	5 cut(s)	1143, 2016, 2763, 2948, 2949
BspPI	GGATC	4 cut(s)	625, 2042, 3013, 3087
BspQI	GCTCTTC	1 cut(s)	2790
BspTNI	GGTCTC	3 cut(s)	303, 2395, 2720
BsrBI	CCGCTC	1 cut(s)	2265
BsrDI	GCAATG	1 cut(s)	2613
BsrFI	RCCGGY	1 cut(s)	3370
BsrGI	TGTACA	1 cut(s)	714
BsrI	ACTGG	3 cut(s)	563, 2089, 2348
BssAI	RCCGGY	1 cut(s)	3370
BssECI	CCNNGG	9 cut(s)	809, 876, 1184, 1258, 1759, 2114, 2708, 2847, 2901
BssNI	GRCGYC	1 cut(s)	1248
BssSI	CACGAG	2 cut(s)	483, 883
BssT1I	CCWWGG	7 cut(s)	809, 876, 1184, 1258, 1759, 2847, 2901
Bst2BI	CACGAG	2 cut(s)	483, 883
Bst2UI	CCWGG	6 cut(s)	47, 210, 1711, 2025, 2709, 3162
Bst6I	CTCTTC	3 cut(s)	1226, 1931, 2790
BstACI	GRCGYC	1 cut(s)	1248
BstAPI	GCANNNNNTGC	2 cut(s)	56, 1682
BstAUI	TGTACA	1 cut(s)	714
BstBAI	YACGTR	2 cut(s)	1062, 3347
BstC8I	GCNNGC	7 cut(s)	349, 870, 1522, 1526, 2739, 3114, 3334
BstDEI	CTNAG	8 cut(s)	108, 537, 570, 1252, 1887, 2543, 3337, 3390
BstDSI	CCRYGG	3 cut(s)	1184, 1258, 1759
BstF5I	GGATG	7 cut(s)	407, 694, 844, 958, 1706, 2973, 3199
BstHHI	GCGC	1 cut(s)	3222
BstMAI	GTCTC	7 cut(s)	303, 880, 1257, 1445, 2395, 2720, 3008
BstNI	CCWGG	6 cut(s)	47, 210, 1711, 2025, 2709, 3162
BstNSI	RCATGY	8 cut(s)	584, 872, 1014, 1032, 1335, 1565, 1680, 1820
BstSCI	CCNGG	8 cut(s)	45, 208, 1709, 2023, 2707, 3059, 3160, 3189
BstSFI	CTRYAG	1 cut(s)	1501
BstSLI	GKGCMC	4 cut(s)	484, 1530, 1971, 2951
BstV2I	GAAGAC	2 cut(s)	1647, 1856
BstX2I	RGATCY	6 cut(s)	7, 617, 3092, 3205, 3258, 3320
BstYI	RGATCY	6 cut(s)	7, 617, 3092, 3205, 3258, 3320
BsuI	GTATCC	1 cut(s)	1116
BtgI	CCRYGG	3 cut(s)	1184, 1258, 1759
BtgZI	GCGATG	1 cut(s)	3093
BtsCI	GGATG	7 cut(s)	407, 694, 844, 958, 1706, 2973, 3199
BtsI	GCAGTG	2 cut(s)	1347, 1969
BtsIMutI	CAGTG	4 cut(s)	1104, 1347, 1904, 1969
Cac8I	GCNNGC	7 cut(s)	349, 870, 1522, 1526, 2739, 3114, 3334
CaiI	CAGNNNCTG	2 cut(s)	569, 2084
CfoI	GCGC	1 cut(s)	3222
Cfr10I	RCCGGY	1 cut(s)	3370
Cfr13I	GGNCC	8 cut(s)	683, 1142, 1181, 2379, 2885, 2947, 2948, 3062
CseI	GACGC	2 cut(s)	1237, 3021
Csp6I	GTAC	6 cut(s)	715, 1074, 1581, 2180, 2597, 3298
CspCI	CAANNNNNGTGG	6 cut(s)	1633, 1668, 2983, 3018, 3289, 3324
CviQI	GTAC	6 cut(s)	715, 1074, 1581, 2180, 2597, 3298
DdeI	CTNAG	8 cut(s)	108, 537, 570, 1252, 1887, 2543, 3337, 3390
DraI	TTTAAA	1 cut(s)	1806
EaeI	YGGCCR	1 cut(s)	2616
Eam1104I	CTCTTC	3 cut(s)	1226, 1931, 2790
EarI	CTCTTC	3 cut(s)	1226, 1931, 2790
EciI	GGCGGA	1 cut(s)	2708
Ecl136II	GAGCTC	1 cut(s)	3362
Eco130I	CCWWGG	7 cut(s)	809, 876, 1184, 1258, 1759, 2847, 2901
Eco147I	AGGCCT	2 cut(s)	351, 417
Eco24I	GRGCYC	3 cut(s)	788, 2951, 3364
Eco31I	GGTCTC	3 cut(s)	303, 2395, 2720
Eco47I	GGWCC	4 cut(s)	683, 1142, 1181, 2379
Eco53kI	GAGCTC	1 cut(s)	3362
Eco57I	CTGAAG	4 cut(s)	1539, 2501, 2561, 3208
Eco88I	CYCGRG	3 cut(s)	787, 1442, 2395
EcoICRI	GAGCTC	1 cut(s)	3362
EcoO109I	RGGNCCY	3 cut(s)	1142, 2947, 2948
EcoRI	GAATTC	2 cut(s)	2581, 3100
EcoRII	CCWGG	6 cut(s)	45, 208, 1709, 2023, 2707, 3160
EcoT14I	CCWWGG	7 cut(s)	809, 876, 1184, 1258, 1759, 2847, 2901
EcoT22I	ATGCAT	1 cut(s)	634
EcoT38I	GRGCYC	3 cut(s)	788, 2951, 3364
ErhI	CCWWGG	7 cut(s)	809, 876, 1184, 1258, 1759, 2847, 2901
Esp3I	CGTCTC	1 cut(s)	1445
FalI	AAGNNNNNCTT	6 cut(s)	348, 380, 1452, 1484, 2800, 2832
FaqI	GGGAC	4 cut(s)	791, 1167, 1466, 2371
FauI	CCCGC	1 cut(s)	1517
FauNDI	CATATG	1 cut(s)	719
FblI	GTMKAC	1 cut(s)	1912
FokI	GGATG	7 cut(s)	414, 701, 851, 965, 1693, 2980, 3206
FriOI	GRGCYC	3 cut(s)	788, 2951, 3364
GlaI	GCGC	1 cut(s)	3221
GsaI	CCCAGC	3 cut(s)	948, 1050, 1089
GsuI	CTGGAG	4 cut(s)	483, 546, 942, 2385
HapII	CCGG	4 cut(s)	686, 3060, 3190, 3371
HgaI	GACGC	2 cut(s)	1237, 3021
HhaI	GCGC	1 cut(s)	3222
Hin1I	GRCGYC	1 cut(s)	1248
Hin6I	GCGC	1 cut(s)	3220
HinP1I	GCGC	1 cut(s)	3220
HincII	GTYRAC	2 cut(s)	2313, 2909
HindII	GTYRAC	2 cut(s)	2313, 2909
HindIII	AAGCTT	1 cut(s)	1800
HpaII	CCGG	4 cut(s)	686, 3060, 3190, 3371
Hpy99I	CGWCG	1 cut(s)	2334
HpyAV	CCTTC	6 cut(s)	61, 943, 2094, 2289, 2464, 3225
HpyCH4IV	ACGT	3 cut(s)	244, 1061, 3346
HpyF3I	CTNAG	8 cut(s)	108, 537, 570, 1252, 1887, 2543, 3337, 3390
HpySE526I	ACGT	3 cut(s)	244, 1061, 3346
Hsp92I	GRCGYC	1 cut(s)	1248
HspAI	GCGC	1 cut(s)	3220
LguI	GCTCTTC	1 cut(s)	2790
LmnI	GCTCC	6 cut(s)	661, 1739, 2020, 2366, 2761, 3359
LweI	GCATC	3 cut(s)	596, 619, 2776
MaeII	ACGT	3 cut(s)	244, 1061, 3346
MaeIII	GTNAC	8 cut(s)	35, 193, 576, 880, 1036, 1171, 1893, 2664
MbiI	CCGCTC	1 cut(s)	2265
MflI	RGATCY	6 cut(s)	7, 617, 3092, 3205, 3258, 3320
MlyI	GAGTC	3 cut(s)	830, 2852, 2884
MmeI	TCCRAC	2 cut(s)	2124, 3249
Mph1103I	ATGCAT	1 cut(s)	634
MroXI	GAANNNNTTC	2 cut(s)	1220, 1275
MslI	CAYNNNNRTG	2 cut(s)	1029, 1764
MspA1I	CMGCKG	1 cut(s)	3341
MspI	CCGG	4 cut(s)	686, 3060, 3190, 3371
MspR9I	CCNGG	8 cut(s)	47, 210, 1711, 2025, 2709, 3061, 3162, 3191
Mva1269I	GAATGC	3 cut(s)	31, 2110, 2803
MvaI	CCWGG	6 cut(s)	47, 210, 1711, 2025, 2709, 3162
NciI	CCSGG	2 cut(s)	3061, 3191
NcoI	CCATGG	3 cut(s)	1184, 1258, 1759
NdeI	CATATG	1 cut(s)	719
NlaIV	GGNNCC	5 cut(s)	1143, 2016, 2763, 2948, 2949
NmuCI	GTSAC	6 cut(s)	35, 193, 576, 880, 1893, 2664
NsiI	ATGCAT	1 cut(s)	634
NspI	RCATGY	8 cut(s)	584, 872, 1014, 1032, 1335, 1565, 1680, 1820
PaeI	GCATGC	1 cut(s)	872
PaeR7I	CTCGAG	1 cut(s)	2395
PceI	AGGCCT	2 cut(s)	351, 417
PciI	ACATGT	3 cut(s)	1028, 1561, 1816
PciSI	GCTCTTC	1 cut(s)	2790
PcsI	WCGNNNNNNNCGW	2 cut(s)	241, 2928
PctI	GAATGC	3 cut(s)	31, 2110, 2803
PdmI	GAANNNNTTC	2 cut(s)	1220, 1275
PflMI	CCANNNNNTGG	1 cut(s)	2024
PfoI	TCCNGGA	4 cut(s)	208, 1709, 2023, 3189
PleI	GAGTC	3 cut(s)	829, 2852, 2884
PpsI	GAGTC	3 cut(s)	829, 2852, 2884
Ppu21I	YACGTR	2 cut(s)	1062, 3347
PpuMI	RGGWCCY	1 cut(s)	1142
PscI	ACATGT	3 cut(s)	1028, 1561, 1816
PsiI	TTATAA	1 cut(s)	2660
Psp124BI	GAGCTC	1 cut(s)	3364
Psp5II	RGGWCCY	1 cut(s)	1142
Psp6I	CCWGG	6 cut(s)	45, 208, 1709, 2023, 2707, 3160
PspFI	CCCAGC	3 cut(s)	944, 1046, 1085
PspGI	CCWGG	6 cut(s)	45, 208, 1709, 2023, 2707, 3160
PspN4I	GGNNCC	5 cut(s)	1143, 2016, 2763, 2948, 2949
PspOMI	GGGCCC	1 cut(s)	2947
PspPI	GGNCC	8 cut(s)	683, 1142, 1181, 2379, 2885, 2947, 2948, 3062
PspPPI	RGGWCCY	1 cut(s)	1142
PstNI	CAGNNNCTG	2 cut(s)	569, 2084
PsuI	RGATCY	6 cut(s)	7, 617, 3092, 3205, 3258, 3320
PvuII	CAGCTG	1 cut(s)	3341
RsaI	GTAC	6 cut(s)	716, 1075, 1582, 2181, 2598, 3299
RsaNI	GTAC	6 cut(s)	715, 1074, 1581, 2180, 2597, 3298
RseI	CAYNNNNRTG	2 cut(s)	1029, 1764
SacI	GAGCTC	1 cut(s)	3364
SapI	GCTCTTC	1 cut(s)	2790
Sau96I	GGNCC	8 cut(s)	683, 1142, 1181, 2379, 2885, 2947, 2948, 3062
ScaI	AGTACT	1 cut(s)	1582
SchI	GAGTC	3 cut(s)	830, 2852, 2884
ScrFI	CCNGG	8 cut(s)	47, 210, 1711, 2025, 2709, 3061, 3162, 3191
SfaNI	GCATC	3 cut(s)	596, 619, 2776
SfcI	CTRYAG	1 cut(s)	1501
Sfr274I	CTCGAG	1 cut(s)	2395
SinI	GGWCC	4 cut(s)	683, 1142, 1181, 2379
SlaI	CTCGAG	1 cut(s)	2395
SmiMI	CAYNNNNRTG	2 cut(s)	1029, 1764
SmlI	CTYRAG	4 cut(s)	1148, 2271, 2395, 2681
SmoI	CTYRAG	4 cut(s)	1148, 2271, 2395, 2681
SpeI	ACTAGT	1 cut(s)	992
SphI	GCATGC	1 cut(s)	872
SseBI	AGGCCT	2 cut(s)	351, 417
SsiI	CCGC	7 cut(s)	295, 1524, 1836, 2263, 2619, 2673, 2693
SstI	GAGCTC	1 cut(s)	3364
StuI	AGGCCT	2 cut(s)	351, 417
StyD4I	CCNGG	8 cut(s)	45, 208, 1709, 2023, 2707, 3059, 3160, 3189
StyI	CCWWGG	7 cut(s)	809, 876, 1184, 1258, 1759, 2847, 2901
TaiI	ACGT	3 cut(s)	247, 1064, 3349
TaqI	TCGA	8 cut(s)	42, 637, 2038, 2186, 2396, 2813, 2856, 3070
TatI	WGTACW	4 cut(s)	714, 1580, 2596, 3297
TauI	GCSGC	1 cut(s)	2621
TscAI	CASTG	4 cut(s)	1111, 1354, 1904, 1976
TseFI	GTSAC	6 cut(s)	35, 193, 576, 880, 1893, 2664
Tsp45I	GTSAC	6 cut(s)	35, 193, 576, 880, 1893, 2664
TspDTI	ATGAA	7 cut(s)	153, 1013, 1731, 2271, 2508, 2808, 3113
TspGWI	ACGGA	3 cut(s)	303, 2371, 3372
TspRI	CASTG	4 cut(s)	1111, 1354, 1904, 1976
Van91I	CCANNNNNTGG	1 cut(s)	2024
VneI	GTGCAC	2 cut(s)	480, 1967
VpaK11BI	GGWCC	4 cut(s)	683, 1142, 1181, 2379
XapI	RAATTY	5 cut(s)	556, 1019, 1134, 2581, 3100
XceI	RCATGY	8 cut(s)	584, 872, 1014, 1032, 1335, 1565, 1680, 1820
XcmI	CCANNNNNNNNNTGG	1 cut(s)	832
XhoI	CTCGAG	1 cut(s)	2395
XmaJI	CCTAGG	1 cut(s)	809
XmiI	GTMKAC	1 cut(s)	1912
XmnI	GAANNNNTTC	2 cut(s)	1220, 1275
ZrmI	AGTACT	1 cut(s)	1582
Zsp2I	ATGCAT	1 cut(s)	634

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.