Rw6G018580

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009215
Physical Location & Seq
Reverse (-)
36894756 .. 36900949
6194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018580.1

Sequence Viewer

Length: 3285 bp
ATGGCAGATCTTGCTAGCTACGGGAATGCCAACCGTGACATTGACCAGGCAATAATTGCTTTGAAGAAGGGTGCTCAGTTACTGAAATATGGTCGTAAGGGAAAGCCTAAGTTTTGTCCATTTCGACTCTCTAGCGACGAATCATCTTTGATCTGGATTTCGAGTAGTGGAGAAAGAACTTTGAAGTTAGCTTCTGTCTCAAGAATTATTCCTGGGCAAAGAACTGCCGTTTTCCAACGATATCTGCGTCCTGAAAAGGATTATTTATCTTTTTCTCTTATATATAATAACGGAAAGCGTTCCCTTGATCTGATTTGCAAGGATAAAGTCGAGGCAGAAGTGTGGATTGCTGGCCTCAAAGCACTTATATCCTCTGGTAGAGGTGGCCGCTCCAAAATTGATGGATGGAGTGATGGAGGCTTGTACCTTGATGATAGCAGAGACTTGACATCAAATAGCCCTAGTGATAGTTCAGTTAGTGGTACACGAGATATTGGGTCTCCTGAGATTTCTGTCAGTTTCAAACCAAATACTTCACCAAAGAGTTTTCCGCCTGACAATTCTCCGGTCTCTGAGAGGTCACATGTAGCATCAGACCAGACAAATATGCAAGTAAAAGGATCCGGTTCAGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCCAGCACTTCAAGTCATGGTTCTGCACCCGATGATTGTGATGCTCTAGGTGATGTATACATATGGGGTGAGGTAATTTGTGATAGTGTTGTTAAGATTGGAGCTGATAAAAATGTAAATTATTTGAGTCCCCGAGCAGATGTGCTTGTTCCTAGGCCATTAGAGTCCAATGTTGTTTTGGATGTACATCATATAGCCTGTGGGGTCAAGCATGCAGCCTTGGTCACAAGACAAGGTGAAGTTTTTACATGGGGTGAAGAATCTGGGGGACGACTTGGCCATGGTGTGGGGAAGGATGTTGCTCAACCCCGTCTAGTTGAATCTCTGGCAGCTACTAGTGTTGATTTTGCGGCGTGTGGAGAATTTCATAGTTGTGCTGTTACAATGGCTGGGGAACTTTATACGTGGGGAGATGGTACACACAATGCTGGGCTTCTTGGTCATGGCACTGATGTCAGTCACTGGATACCAAAGAGAATTTCAGGTCCTCTTGAGGGACTTCAAGTTGCTTCTGTATCTTGTGGTCCATGGCATACGGCCTTGGTGACATCAACGGGGAAACTTTTTACATTTGGCGATGGAACATTTGGTGTCTTAGGCCATGGAGACAGAGAAAATGTTTCATATCCGAGAGAAGTAGATTCCCTAACAGGTTTGAGGACAATTGCTGTTGCATGTGGGGTATGGCACACTGCTGCTGTAGTAGAGGTTATTGCAACACAATCTAGTGCCAGTATTTCATCAGGTAAATTGTTTACTTGGGGTGATGGAGATAAAAATCGTCTTGGACATGGAGACAAGGAAGCCCGGCTTAAACCGACGTGTGTTCCAGCCCTTATTGATTACAATTTTCACAAAATTGCTTGCGGGCACAGTTTAACTGTTGGCTTGACAACATCGGGGCATGTCTTTACAATGGGTAGTACTGTTTATGGTCAGCTCGGAAATCCCAATTCTGATGGAAAAATACCTTGCTTAGTGGATGACAAACTTTCCGGGGAATGTATTGAAGAAATTGCTTGTGGTGCATATCATGTAGCAGTATTAACATCCAGAAATGAAGTTTATACATGGGGAAAAGGTGCTAATGGGAGGTTGGGCCATGGTGATGTTGAGGATCGAAAAACACCGACTATGGTCGAAGGTTTGAAGGATAGACATGTGAAATATATTGGTTGTGGTTCACACTATACAGCAGCTATATGTCTTCACAAATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGATTTACAAGGAAGAGGCATAATTGCTATAATTCAACAAGAGCAGCACTGTCTCCTAATCCTGGAAAACCATATCGAGTGTGTGATTCTTGTTATGTGAAATTGAACAAGGTGTTAGAACCTGGTAGTAATAATAGAAAGAATGTTATACCTCGCTTGTCTGGTGAGAACAAGGACAGATTGGACAAGGCTGACATAAGATTATACAAGTCTGCTGTACCTTCAAACGTGGATTTGATAAAGCAATTAGATAGCAAAGCAGCCAAGCAAGGAAAGAAAGCTGAGACATTTTCTCTTGTTCGCTCCTCTCAAGCACCTTCGCTGTTGCAATTAAAGGATGTAGTTATGTCAGCTGCTGTTGATCTGAGACGAACAGTTCCCAAACCAGTTCTTACACCGTCTGGAGTAAGTTCGAGGTCTGTGTCGCCTTTCTCAAGGAGACCAAGCCCTCCTCGTTTTGCAACTCCTGTTCCTACAACTTCTGGACTTTCCTTCTCAAAAAGTATCGCAGATAGTTTGAAGAAAACTAATGAGCTTTTGAATCAGGAAGTGCTAAAGTTACGTTCACAGGTTGATAGCCTGAGACAGAGATGTGAACTTCAAGAAGTAGAGCTTCAGAATTCAACAAAGAAAGCTCAAGAAGCTATGACAGTGGCTGCAGAGGAATCTGCGAAATCTAAAGCGGCTAAAGAAGTTATAAAGTCACTAACTGCACAGCTCAAGGATTTGGCTGAAAGGCTGCCACCTGGGGTTTATGATACTGAGGGCATAAACAGGCATATCCATCTTATGGTTTGGAATAACCATTCAAGATCAAGTTCAATGAGCAATTCTTACTTAATTTCCTCCATGGGAATCGACTCAACTACAGTAAACGGAAGTCTAGGGCAGACTCATTCACCCAGGGATTCAGTAGGAACGAATGAAACTAACGTGCAACACAATAGGGAGCTGGTGACCTCCAATGGGATGGTAAATGCACTAGATAGACTGCCCAATGGTGGTGGATCATTTCAGTCAGTTGGTAGTAATCTGTCGGTGGCTGTTGATGGCAAGGACTCTGGGCCTTTCCAAGATGGGGAGAATGGTACGAGATCTAGGAATTCTAATGGATTGAACAATATGAGCCTGGGTGTTTACATAACTCTTGTGGCTCTTCGTGATGGAACTAGAGATCTCAAACGAGTGCGCTTCAGCCGGAGAAGATTTGGAGAACACCAAGCAGAGATTTGGTGGTCTGAGAATCGCGAAAAAGTGTACGAGAAGTACAACGTCCGTGGATCGGACAAGTCTTCAGTTTCTGGCTCAGCAGCACGTAGATCGGATGGAGCTCTCTCACCTGCATCCCAACAACCCTAG

Protein Analysis

1094

Amino Acids

117.99

Weight (kDa)

8.99

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 123 1.8e-08 Pleckstrin homology domain
WD40_RLD PF25390 239 - 620 4e-77 RCC1-like domain
RCC1 PF00415 298 - 347 6.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 351 - 402 5.1e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 389 - 417 1.6e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 406 - 454 1.4e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 468 - 516 8e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 520 - 569 6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 557 - 584 5.3e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 574 - 621 8.3e-14 Regulator of chromosome condensation (RCC1) repeat
BRX_N PF13713 863 - 897 4.9e-18 Transcription factor BRX N-terminal domain
BRX PF08381 1017 - 1065 1.9e-23 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2624
AarI CACCTGC 1 cut(s) 3274
Acc36I ACCTGC 1 cut(s) 3274
AccB7I CCANNNNNTGG 2 cut(s) 946, 2716
AccBSI CCGCTC 1 cut(s) 390
AccI GTMKAC 2 cut(s) 717, 1915
AccII CGCG 1 cut(s) 3174
AciI CCGC 5 cut(s) 388, 551, 1010, 1527, 2609
AclWI GGATC 5 cut(s) 615, 628, 1785, 2941, 3214
AcoI YGGCCR 2 cut(s) 385, 937
AcsI RAATTY 4 cut(s) 1022, 1137, 2545, 3028
AcuI CTGAAG 3 cut(s) 2525, 3103, 3204
AfaI GTAC 9 cut(s) 425, 484, 846, 1078, 1585, 2145, 3016, 3185, 3194
AfiI CCNNNNNNNGG 8 cut(s) 946, 1154, 1988, 2716, 2892, 2927, 3004, 3208
AflIII ACRYGT 3 cut(s) 583, 1481, 1819
AhlI ACTAGT 1 cut(s) 995
AjiI CACGTC 1 cut(s) 1482
AjnI CCWGG 7 cut(s) 45, 211, 1987, 2047, 2671, 2828, 3054
AjuI GAANNNNNNNTTGG 2 cut(s) 1123, 1155
Alw21I GWGCWC 4 cut(s) 76, 661, 1907, 3259
AlwI GGATC 5 cut(s) 615, 628, 1785, 2941, 3214
AlwNI CAGNNNCTG 5 cut(s) 82, 1122, 2282, 2582, 3227
Ama87I CYCGRG 1 cut(s) 792
AoxI GGCC 8 cut(s) 352, 385, 815, 937, 1197, 1258, 1759, 2990
ApoI RAATTY 4 cut(s) 1022, 1137, 2545, 3028
Asp700I GAANNNNTTC 1 cut(s) 298
AspA2I CCTAGG 1 cut(s) 812
AspLEI GCGC 1 cut(s) 3117
AspS9I GGNCC 4 cut(s) 1145, 1184, 1759, 2990
AsuC2I CCSGG 2 cut(s) 1468, 1657
AsuNHI GCTAGC 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 2 cut(s) 1145, 1184
AvrII CCTAGG 1 cut(s) 812
BaeGI GKGCMC 1 cut(s) 1533
BalI TGGCCA 1 cut(s) 939
BamHI GGATCC 1 cut(s) 620
BanII GRGCYC 1 cut(s) 3259
BauI CACGAG 1 cut(s) 486
BbsI GAAGAC 2 cut(s) 1859, 3210
Bbv12I GWGCWC 4 cut(s) 76, 661, 1907, 3259
BceAI ACGGC 2 cut(s) 212, 1212
BcgI CGANNNNNNTGC 2 cut(s) 3228, 3262
BciT130I CCWGG 7 cut(s) 47, 213, 1989, 2049, 2673, 2830, 3056
BciVI GTATCC 1 cut(s) 1119
BcnI CCSGG 2 cut(s) 1468, 1657
BcuI ACTAGT 1 cut(s) 995
BfmI CTRYAG 3 cut(s) 1359, 2583, 2793
BfuAI ACCTGC 1 cut(s) 3274
BfuI GTATCC 1 cut(s) 1119
BglII AGATCT 3 cut(s) 7, 3020, 3100
BlnI CCTAGG 1 cut(s) 812
BlpI GCTNAGC 2 cut(s) 1890, 3232
BmcAI AGTACT 1 cut(s) 1585
Bme1390I CCNGG 9 cut(s) 47, 213, 1468, 1657, 1989, 2049, 2673, 2830, 3056
Bme18I GGWCC 2 cut(s) 1145, 1184
BmeT110I CYCGRG 1 cut(s) 792
BmgBI CACGTC 1 cut(s) 1482
BmgT120I GGNCC 4 cut(s) 1145, 1184, 1759, 2990
BmiI GGNNCC 1 cut(s) 622
BmrFI CCNGG 9 cut(s) 47, 213, 1468, 1657, 1989, 2049, 2673, 2830, 3056
BmsI GCATC 4 cut(s) 599, 622, 691, 3278
BmtI GCTAGC 1 cut(s) 18
BoxI GACNNNNGTC 1 cut(s) 1796
BpiI GAAGAC 2 cut(s) 1859, 3210
BpmI CTGGAG 1 cut(s) 2349
Bpu1102I GCTNAGC 2 cut(s) 1890, 3232
BpuEI CTTGAG 6 cut(s) 184, 1172, 2220, 2344, 2547, 2630
BpuMI CCSGG 2 cut(s) 1468, 1657
BsaAI YACGTR 2 cut(s) 1065, 3242
BsaBI GATNNNNATC 2 cut(s) 846, 1437
BsaI GGTCTC 3 cut(s) 504, 574, 2359
BsaWI WCCGGW 2 cut(s) 565, 623
Bsc4I CCNNNNNNNGG 8 cut(s) 946, 1154, 1988, 2716, 2892, 2927, 3004, 3208
Bse1I ACTGG 3 cut(s) 1127, 1392, 2312
Bse8I GATNNNNATC 2 cut(s) 846, 1437
BseBI CCWGG 7 cut(s) 47, 213, 1989, 2049, 2673, 2830, 3056
BseGI GGATG 9 cut(s) 410, 847, 961, 1648, 1709, 2269, 2901, 3256, 3269
BseJI GATNNNNATC 2 cut(s) 846, 1437
BseLI CCNNNNNNNGG 8 cut(s) 946, 1154, 1988, 2716, 2892, 2927, 3004, 3208
BseNI ACTGG 3 cut(s) 1127, 1392, 2312
BseRI GAGGAG 2 cut(s) 2221, 2367
BseSI GKGCMC 1 cut(s) 1533
BseYI CCCAGC 3 cut(s) 662, 1049, 1088
BsgI GTGCAG 2 cut(s) 669, 2622
Bsh1236I CGCG 1 cut(s) 3174
BshFI GGCC 8 cut(s) 354, 387, 817, 939, 1199, 1260, 1761, 2992
BsiHKAI GWGCWC 4 cut(s) 76, 661, 1907, 3259
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 5 cut(s) 566, 624, 1468, 1656, 3124
BslFI GGGAC 3 cut(s) 774, 942, 1170
BslI CCNNNNNNNGG 8 cut(s) 946, 1154, 1988, 2716, 2892, 2927, 3004, 3208
BsmBI CGTCTC 1 cut(s) 2287
BsmFI GGGAC 3 cut(s) 774, 942, 1170
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 8 cut(s) 354, 387, 817, 939, 1199, 1260, 1761, 2992
Bso31I GGTCTC 3 cut(s) 504, 574, 2359
BsoBI CYCGRG 1 cut(s) 792
Bsp1286I GDGCHC 5 cut(s) 76, 661, 1533, 1907, 3259
Bsp1407I TGTACA 1 cut(s) 844
Bsp1720I GCTNAGC 2 cut(s) 1890, 3232
Bsp19I CCATGG 5 cut(s) 940, 1187, 1261, 1762, 2775
Bsp68I TCGCGA 1 cut(s) 3174
BspACI CCGC 5 cut(s) 388, 551, 1010, 1527, 2609
BspANI GGCC 8 cut(s) 354, 387, 817, 939, 1199, 1260, 1761, 2992
BspFNI CGCG 1 cut(s) 3174
BspLI GGNNCC 1 cut(s) 622
BspMAI CTGCAG 1 cut(s) 2587
BspMI ACCTGC 1 cut(s) 3274
BspOI GCTAGC 1 cut(s) 18
BspPI GGATC 5 cut(s) 615, 628, 1785, 2941, 3214
BspQI GCTCTTC 1 cut(s) 3087
BspTNI GGTCTC 3 cut(s) 504, 574, 2359
BsrBI CCGCTC 1 cut(s) 390
BsrGI TGTACA 1 cut(s) 844
BsrI ACTGG 3 cut(s) 1127, 1392, 2312
BssNAI GTATAC 1 cut(s) 718
BssSI CACGAG 1 cut(s) 486
BssT1I CCWWGG 8 cut(s) 812, 879, 940, 1187, 1200, 1261, 1762, 2775
Bst1107I GTATAC 1 cut(s) 718
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 7 cut(s) 47, 213, 1989, 2049, 2673, 2830, 3056
Bst6I CTCTTC 2 cut(s) 1934, 3087
BstAPI GCANNNNNTGC 2 cut(s) 11, 56
BstAUI TGTACA 1 cut(s) 844
BstBAI YACGTR 2 cut(s) 1065, 3242
BstC8I GCNNGC 5 cut(s) 16, 352, 873, 1525, 1529
BstDSI CCRYGG 6 cut(s) 940, 1187, 1261, 1762, 2775, 3202
BstEII GGTNACC 1 cut(s) 2881
BstF5I GGATG 9 cut(s) 410, 847, 961, 1648, 1709, 2269, 2901, 3256, 3269
BstFNI CGCG 1 cut(s) 3174
BstHHI GCGC 1 cut(s) 3117
BstMWI GCNNNNNNNGC 4 cut(s) 11, 56, 1685, 2567
BstNI CCWGG 7 cut(s) 47, 213, 1989, 2049, 2673, 2830, 3056
BstNSI RCATGY 5 cut(s) 587, 875, 1338, 1568, 1823
BstPAI GACNNNNGTC 1 cut(s) 1796
BstPI GGTNACC 1 cut(s) 2881
BstSCI CCNGG 9 cut(s) 45, 211, 1466, 1655, 1987, 2047, 2671, 2828, 3054
BstSFI CTRYAG 3 cut(s) 1359, 2583, 2793
BstSLI GKGCMC 1 cut(s) 1533
BstUI CGCG 1 cut(s) 3174
BstV2I GAAGAC 2 cut(s) 1859, 3210
BstX2I RGATCY 4 cut(s) 7, 620, 3020, 3100
BstXI CCANNNNNNTGG 1 cut(s) 2896
BstYI RGATCY 4 cut(s) 7, 620, 3020, 3100
BstZ17I GTATAC 1 cut(s) 718
BsuI GTATCC 1 cut(s) 1119
BsuRI GGCC 8 cut(s) 354, 387, 817, 939, 1199, 1260, 1761, 2992
BtgI CCRYGG 6 cut(s) 940, 1187, 1261, 1762, 2775, 3202
BtgZI GCGATG 1 cut(s) 1251
BtrI CACGTC 1 cut(s) 1482
BtsCI GGATG 9 cut(s) 410, 847, 961, 1648, 1709, 2269, 2901, 3256, 3269
BtsI GCAGTG 1 cut(s) 1350
BtsIMutI CAGTG 6 cut(s) 1107, 1120, 1350, 1907, 1973, 2583
BtuMI TCGCGA 1 cut(s) 3174
BveI ACCTGC 1 cut(s) 3274
Cac8I GCNNGC 5 cut(s) 16, 352, 873, 1525, 1529
CaiI CAGNNNCTG 5 cut(s) 82, 1122, 2282, 2582, 3227
CfoI GCGC 1 cut(s) 3117
Cfr13I GGNCC 4 cut(s) 1145, 1184, 1759, 2990
CseI GACGC 1 cut(s) 236
CsiI ACCWGGT 1 cut(s) 2047
Csp6I GTAC 9 cut(s) 424, 483, 845, 1077, 1584, 2144, 3015, 3184, 3193
CspCI CAANNNNNGTGG 4 cut(s) 2911, 2946, 3184, 3219
CviQI GTAC 9 cut(s) 424, 483, 845, 1077, 1584, 2144, 3015, 3184, 3193
EaeI YGGCCR 2 cut(s) 385, 937
Eam1104I CTCTTC 2 cut(s) 1934, 3087
EarI CTCTTC 2 cut(s) 1934, 3087
EciI GGCGGA 1 cut(s) 540
Ecl136II GAGCTC 1 cut(s) 3257
Eco130I CCWWGG 8 cut(s) 812, 879, 940, 1187, 1200, 1261, 1762, 2775
Eco24I GRGCYC 1 cut(s) 3259
Eco31I GGTCTC 3 cut(s) 504, 574, 2359
Eco32I GATATC 1 cut(s) 242
Eco47I GGWCC 2 cut(s) 1145, 1184
Eco53kI GAGCTC 1 cut(s) 3257
Eco57I CTGAAG 3 cut(s) 2525, 3103, 3204
Eco88I CYCGRG 1 cut(s) 792
Eco91I GGTNACC 1 cut(s) 2881
EcoICRI GAGCTC 1 cut(s) 3257
EcoO109I RGGNCCY 1 cut(s) 1145
EcoO65I GGTNACC 1 cut(s) 2881
EcoRI GAATTC 2 cut(s) 2545, 3028
EcoRII CCWGG 7 cut(s) 45, 211, 1987, 2047, 2671, 2828, 3054
EcoRV GATATC 1 cut(s) 242
EcoT14I CCWWGG 8 cut(s) 812, 879, 940, 1187, 1200, 1261, 1762, 2775
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 3259
ErhI CCWWGG 8 cut(s) 812, 879, 940, 1187, 1200, 1261, 1762, 2775
Esp3I CGTCTC 1 cut(s) 2287
FalI AAGNNNNNCTT 4 cut(s) 1455, 1487, 2523, 2555
FaqI GGGAC 3 cut(s) 774, 942, 1170
FauI CCCGC 1 cut(s) 1520
FauNDI CATATG 1 cut(s) 722
FblI GTMKAC 2 cut(s) 717, 1915
FokI GGATG 9 cut(s) 417, 854, 968, 1655, 1696, 2276, 2908, 3256, 3263
FriOI GRGCYC 1 cut(s) 3259
GlaI GCGC 1 cut(s) 3116
GsaI CCCAGC 3 cut(s) 666, 1053, 1092
GsuI CTGGAG 1 cut(s) 2349
HaeIII GGCC 8 cut(s) 354, 387, 817, 939, 1199, 1260, 1761, 2992
HapII CCGG 5 cut(s) 566, 624, 1468, 1656, 3124
HgaI GACGC 1 cut(s) 236
HhaI GCGC 1 cut(s) 3117
Hin6I GCGC 1 cut(s) 3115
HinP1I GCGC 1 cut(s) 3115
HpaII CCGG 5 cut(s) 566, 624, 1468, 1656, 3124
Hpy99I CGWCG 2 cut(s) 140, 1483
HpyAV CCTTC 7 cut(s) 61, 946, 1796, 1804, 2157, 2253, 2428
HpyCH4IV ACGT 7 cut(s) 1064, 1481, 2154, 2488, 2859, 3198, 3241
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 56, 1685, 2567
HpySE526I ACGT 7 cut(s) 1064, 1481, 2154, 2488, 2859, 3198, 3241
HspAI GCGC 1 cut(s) 3115
LguI GCTCTTC 1 cut(s) 3087
LmnI GCTCC 6 cut(s) 395, 664, 761, 2234, 2875, 3254
LweI GCATC 4 cut(s) 599, 622, 691, 3278
MabI ACCWGGT 1 cut(s) 2047
MaeII ACGT 7 cut(s) 1064, 1481, 2154, 2488, 2859, 3198, 3241
MbiI CCGCTC 1 cut(s) 390
MboII GAAGA 9 cut(s) 76, 929, 1682, 1859, 1951, 2458, 3074, 3141, 3210
MfeI CAATTG 1 cut(s) 1323
MflI RGATCY 4 cut(s) 7, 620, 3020, 3100
MhlI GDGCHC 5 cut(s) 76, 661, 1533, 1907, 3259
MlsI TGGCCA 1 cut(s) 939
MluNI TGGCCA 1 cut(s) 939
MlyI GAGTC 6 cut(s) 120, 796, 833, 2780, 2812, 2978
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 939
Mph1103I ATGCAT 1 cut(s) 637
MroXI GAANNNNTTC 1 cut(s) 298
MscI TGGCCA 1 cut(s) 939
MseI TTAA 6 cut(s) 753, 1473, 1538, 1706, 2258, 2765
MslI CAYNNNNRTG 2 cut(s) 1032, 1767
Msp20I TGGCCA 1 cut(s) 939
MspA1I CMGCKG 1 cut(s) 2279
MspI CCGG 5 cut(s) 566, 624, 1468, 1656, 3124
MspR9I CCNGG 9 cut(s) 47, 213, 1468, 1657, 1989, 2049, 2673, 2830, 3056
MunI CAATTG 1 cut(s) 1323
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 7 cut(s) 47, 213, 1989, 2049, 2673, 2830, 3056
MvnI CGCG 1 cut(s) 3174
MwoI GCNNNNNNNGC 4 cut(s) 11, 56, 1685, 2567
NciI CCSGG 2 cut(s) 1468, 1657
NcoI CCATGG 5 cut(s) 940, 1187, 1261, 1762, 2775
NdeI CATATG 1 cut(s) 722
NheI GCTAGC 1 cut(s) 14
NlaIV GGNNCC 1 cut(s) 622
NmuCI GTSAC 8 cut(s) 35, 579, 883, 1118, 1204, 1896, 2628, 2881
NruI TCGCGA 1 cut(s) 3174
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 5 cut(s) 587, 875, 1338, 1568, 1823
PaeI GCATGC 1 cut(s) 875
PaqCI CACCTGC 1 cut(s) 3274
PasI CCCWGGG 1 cut(s) 2829
PciI ACATGT 2 cut(s) 583, 1819
PciSI GCTCTTC 1 cut(s) 3087
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PctI GAATGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 298
PflMI CCANNNNNTGG 2 cut(s) 946, 2716
PfoI TCCNGGA 1 cut(s) 1987
PleI GAGTC 6 cut(s) 120, 795, 832, 2780, 2812, 2978
PpsI GAGTC 6 cut(s) 120, 795, 832, 2780, 2812, 2978
Ppu21I YACGTR 2 cut(s) 1065, 3242
PpuMI RGGWCCY 1 cut(s) 1145
PscI ACATGT 2 cut(s) 583, 1819
PshAI GACNNNNGTC 1 cut(s) 1796
PsiI TTATAA 1 cut(s) 2624
Psp124BI GAGCTC 1 cut(s) 3259
Psp5II RGGWCCY 1 cut(s) 1145
Psp6I CCWGG 7 cut(s) 45, 211, 1987, 2047, 2671, 2828, 3054
PspEI GGTNACC 1 cut(s) 2881
PspFI CCCAGC 3 cut(s) 662, 1049, 1088
PspGI CCWGG 7 cut(s) 45, 211, 1987, 2047, 2671, 2828, 3054
PspN4I GGNNCC 1 cut(s) 622
PspPI GGNCC 4 cut(s) 1145, 1184, 1759, 2990
PspPPI RGGWCCY 1 cut(s) 1145
PstI CTGCAG 1 cut(s) 2587
PstNI CAGNNNCTG 5 cut(s) 82, 1122, 2282, 2582, 3227
PsuI RGATCY 4 cut(s) 7, 620, 3020, 3100
PvuII CAGCTG 1 cut(s) 2279
RruI TCGCGA 1 cut(s) 3174
RsaI GTAC 9 cut(s) 425, 484, 846, 1078, 1585, 2145, 3016, 3185, 3194
RsaNI GTAC 9 cut(s) 424, 483, 845, 1077, 1584, 2144, 3015, 3184, 3193
RseI CAYNNNNRTG 2 cut(s) 1032, 1767
SacI GAGCTC 1 cut(s) 3259
SapI GCTCTTC 1 cut(s) 3087
SaqAI TTAA 6 cut(s) 753, 1473, 1538, 1706, 2258, 2765
Sau96I GGNCC 4 cut(s) 1145, 1184, 1759, 2990
ScaI AGTACT 1 cut(s) 1585
SchI GAGTC 6 cut(s) 120, 796, 833, 2780, 2812, 2978
ScrFI CCNGG 9 cut(s) 47, 213, 1468, 1657, 1989, 2049, 2673, 2830, 3056
SduI GDGCHC 5 cut(s) 76, 661, 1533, 1907, 3259
SexAI ACCWGGT 1 cut(s) 2047
SfaNI GCATC 4 cut(s) 599, 622, 691, 3278
SfcI CTRYAG 3 cut(s) 1359, 2583, 2793
SinI GGWCC 2 cut(s) 1145, 1184
SmiMI CAYNNNNRTG 2 cut(s) 1032, 1767
SmlI CTYRAG 6 cut(s) 199, 1151, 2235, 2359, 2562, 2645
SmoI CTYRAG 6 cut(s) 199, 1151, 2235, 2359, 2562, 2645
SpeI ACTAGT 1 cut(s) 995
SphI GCATGC 1 cut(s) 875
SsiI CCGC 5 cut(s) 388, 551, 1010, 1527, 2609
SstI GAGCTC 1 cut(s) 3259
StyD4I CCNGG 9 cut(s) 45, 211, 1466, 1655, 1987, 2047, 2671, 2828, 3054
StyI CCWWGG 8 cut(s) 812, 879, 940, 1187, 1200, 1261, 1762, 2775
TaiI ACGT 7 cut(s) 1067, 1484, 2157, 2491, 2862, 3201, 3244
TaqI TCGA 9 cut(s) 124, 161, 330, 640, 1780, 1800, 2002, 2339, 2784
TatI WGTACW 3 cut(s) 844, 1583, 3192
TauI GCSGC 3 cut(s) 390, 1013, 2612
Tru1I TTAA 6 cut(s) 753, 1473, 1538, 1706, 2258, 2765
Tru9I TTAA 6 cut(s) 753, 1473, 1538, 1706, 2258, 2765
TscAI CASTG 6 cut(s) 1114, 1127, 1357, 1907, 1980, 2583
TseFI GTSAC 8 cut(s) 35, 579, 883, 1118, 1204, 1896, 2628, 2881
Tsp45I GTSAC 8 cut(s) 35, 579, 883, 1118, 1204, 1896, 2628, 2881
TspDTI ATGAA 5 cut(s) 1016, 1272, 1389, 1734, 2865
TspGWI ACGGA 3 cut(s) 306, 2817, 3191
TspRI CASTG 6 cut(s) 1114, 1127, 1357, 1907, 1980, 2583
Van91I CCANNNNNTGG 2 cut(s) 946, 2716
VpaK11BI GGWCC 2 cut(s) 1145, 1184
XapI RAATTY 4 cut(s) 1022, 1137, 2545, 3028
XceI RCATGY 5 cut(s) 587, 875, 1338, 1568, 1823
XcmI CCANNNNNNNNNTGG 1 cut(s) 835
XmaJI CCTAGG 1 cut(s) 812
XmiI GTMKAC 2 cut(s) 717, 1915
XmnI GAANNNNTTC 1 cut(s) 298
ZrmI AGTACT 1 cut(s) 1585
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.