FvH4_2g14280

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009215
Physical Location & Seq
Reverse (-)
12546995 .. 12554121
7127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14280.t1

Sequence Viewer

Length: 3381 bp
ATGGCAGATCTTGTTAGCTACGGCAACGCCAATCGTGACATTGACCAGGCAATAATTTCTTTGAAGAAGGGTGCTCAGCTACTGAAATATGGTCGTAAGGGAAAGCCTAAGTTTTGTCCGTTTCGACTCTCTAGTGACGAATCATCTTTGATCTGGATTTCGAGTAGTGGAGAAAGAACTTTGAAGTTAGCTTCTGTCTCAAGAATTATTCCTGGACAAAGAACTGCTGTTTTCCAACGATATCTGCGGCCTGAAAAAGACTATTTATCTTTTTCTCTTATATATAATAACGGAAAGCGGTCCCTTGATCTGATTTGTAAGGATAAAGTTGAGGCAGAAGTGTGGATTGCTGGCCTCAAGGCACTTATATCCTCCGGTACAGGTGGTCGCTCCAAAATTGATGGATGGAGTGATGGAGGCTTGTACCTTGATGATAGCAGAGACTTGACATCAAATAGCCCTAGTGATAGTTCAGTTAGTGGTGCACGAGATAGTGGCTCTCCTGAGATTTCTGTCAATTTTAAACCAAATACTTCACCAAAGAGTTTTCCACCTGACAATTCTCCAGTCTCCGAGAGGTCGCATGTAGCATCAGAGAAGACAAATATGCAAGTAAAAGGTTCAGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCCAGCACTTCAAGTCATGGTTCTGCACCCGATGATTGTGATGCTCTAGGTGATGTATACATATGGGGTGAGGTCATTTGTGACAGTGTTGTTAAGATTGGAGCTGATAAAAATGTAAATTATTCAAGTCCCCGAGCAGATGTGCTTGTTCCTAGGCCCTTAGAGTGCAATGTTGTTTTGGATGTACATCATATAGCCTGTGGGGTCAAGCATGCAGCCTTGGTCACAAGACAAGGTGAAGTTTTTACATGGGGTGAAGAATCTGGGGGAAGACTTGGCCATGGTGTGGGGAAAGATGTTGCTCAACCTTGTCTGGTTGAATCTCTGGCAGCTACTAATGTTGATTTTGCGGCATGTGGAGAATTTCATAGTTGTGCTGTTACAATGGCTGGGGAGCTTTATACATGGGGAGATGGTACACACAATGCTGGGCTTCTTGGTCATGGCACTGATGTCAGTCACTGGATACCAAAGAGAATTTCAGGTCCTCTTGAGGGACTTCAAGTTGCTTCTGTATCATGTGGTCCATGGCATACGGCCGTGGTGACATCAACAGGGAAACTTTTTACATTTGGCGATGGAACATTTGGTGTCTTAGGCCATGGAGACAGAGGAAATGTTTCATATCCGAGAGAAGTAGATTCCCTATCAGGATTGAGGACAATAGCTGTTGCATGTGGGGTGTGGCACACTGCTGCTGTAGTGGAGGTTATCGCGACACAATCTAGTGCCAGTATTTCATCAGGTAAATTGTTTACTTGGGGTGACGGAGATAAAAATCGTCTTGGACATGGAGACAAGGAAGCCCGGCTTAAACCGACATGTGTTCCAGCCCTTATTGATTACAATTTTCATAAAATTGCTTGCGGGCACAGTTTAACTGTTGGCTTGACAACATCAGGGCATGTCTTTACAATGGGTAGTACTGTTTATGGTCAGCTTGGGAATCCCAATTCTGATGGAAAACTACCTTGCTTGGTGGATGACAAACTTTCCGGGGAATGTATTGAAGAAATTGCTTGTGGTGCATATCATGTAGCAGTATTAACATCCAGAAATGAAGTTTATACGTGGGGAAAAGGTGCTAATGGGAGGTTGGGGCATGGTGATGTTGAGGATCGAAAGACACCGACTTTGGTTGAAGGTTTAAAGGATAGACATGTGAAATATATTGGTTGTGGTTCACACTATACAGCAGCTATATGTCTTCACAGATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGATTTACAAGGAAGAGGCATAATTGCTATAACTGTGGACTTGTGCACTGCCATTCATGTAGTTCCAGAAAAGCAACAAGAGCAGCACTGTCTCCTAATCCTGGAAAACCATATCGAGTGTGTGATTCTTGTTATGTGAAATTGAACAAGGTGTTAGAACCTGGTAGTAATAATAGAAAGAATGTTATACCTCGCTTGTCTGGTGAGAACAAGGACAGATTGGACAAGGCTGAGATAAGATTATACAAGTCTGCCGTACCTTCAAACGTGGATTTGATAAAGCAATTAGATAGCAAAGCAGCCAAGCAAGGAAAGAAAGCTGAGACATTTTCCCTTGTTCGCTCCTCTCAAACACCTTCGCTGTTGCAATTAAAGGATGTAGTTATGTCAGCTGCTGTTGATCTGAGACGAACAGTGCCCAAACCAGTTCTTACACCATCTGGAGTAAGTTCGAGGTCTGTGTCACCGTTCTCAAGGAGACCAAGCCCTCCTCGTTTTGCAACTCCTGTTCCTACAACATCCGGACTTTCCTTCTCTAAAAGTATTGCAGATAGTTTGAAGAAAACTAATGAGCTTTTGAACCAAGAAGTGCTAAAGTTACGTTCGCAGGTTGATAGCTTGAGACAGAGATGTGAACTTCAAGAAGAAGAGCTTCAGAATTCAACAAAGAAAACACAAGAAGCTATGGCAATGGCTGCAGAAGAATCTGCCAAATCTAAAGCGGCTAAAGAAGTTATAAAGTCACTAACCGCACAGCTCAAGGATTTGGCTGAAAGGTTGCCACCTGGGGTTTATGATAGCGAGAGCATAAAGAAGGCATATCCATCTAACGGTTTGGAGCCAAATGGTATTCATTATCCAGATGGAGATAACCATTCAAGATCAAGTTCAATGAGCAATTCTTACTTAATTTCCTCCATGGGAATTGACTCAACTACAGTTAATGGAAGTCGAGGGCAGACTCATTCACCCAGGGATTCTGTAGGAACGAATGAAACTAACCTACAACACAATAGGGAGCTGGTGACCTCCAATGGGATGGTGAATGCTTTAGATAGACTGCCCAATGGTGGCGGATCATTTCAGTCAGTTGGTAGTAATCTGTCAGTGGCCGTTGATGGCAAGGACTCTGGGCCTATCCAAGATGGGGAGAATGGTACGAGATCTAGGAACCCTACATTGGCTGTTAGTGGTAATACAGTTGAGGCAGAATGGATTGAACAATATGAGCCTGGTGTTTACATAACTCTTGTGGCTCTCCGCGATGGAACTAGAGATCTCAAACGAGTGCGCTTCAGCCGGAGAAGATTTGGAGAGCACCAAGCAGAGATTTGGTGGTCCGAGAACCGTGAGAAAGTGTACGAGAAGTACAACGTCCGTGGGTCAGACAAGTCTTCAGTTTCTGGCTCAGCGTCTCGTAGATCGGATGGAGCTCTCTCACCTGCACCCCAACAACCTTAG

Protein Analysis

1127

Amino Acids

121.38

Weight (kDa)

8.76

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 16 - 123 1.9e-08 Pleckstrin homology domain
WD40_RLD PF25390 237 - 618 2.2e-76 RCC1-like domain
RCC1 PF00415 296 - 345 8.4e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 349 - 400 4e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 387 - 415 5.1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 404 - 452 4.3e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 466 - 514 8.3e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 518 - 567 8.8e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 555 - 582 5.5e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 572 - 619 1.3e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 623 - 689 5.1e-15 FYVE zinc finger
BRX_N PF13713 874 - 908 4.7e-18 Transcription factor BRX N-terminal domain
BRX PF08381 1042 - 1097 7.8e-29 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2657
AarI CACCTGC 1 cut(s) 3370
Acc36I ACCTGC 2 cut(s) 2518, 3370
AccB7I CCANNNNNTGG 1 cut(s) 940
AccI GTMKAC 2 cut(s) 711, 1909
AccII CGCG 2 cut(s) 1370, 3183
AccIII TCCGGA 1 cut(s) 2441
AciI CCGC 8 cut(s) 247, 298, 1004, 1521, 2642, 2670, 2994, 3181
AclWI GGATC 2 cut(s) 1779, 3004
AcoI YGGCCR 3 cut(s) 931, 1191, 3030
AcsI RAATTY 3 cut(s) 1016, 1131, 2578
AcuI CTGAAG 3 cut(s) 2558, 3199, 3300
AfaI GTAC 9 cut(s) 379, 425, 840, 1072, 1579, 2178, 3079, 3281, 3290
AfiI CCNNNNNNNGG 8 cut(s) 940, 1148, 2021, 2750, 2955, 2990, 3067, 3100
AflIII ACRYGT 2 cut(s) 1475, 1813
AjnI CCWGG 7 cut(s) 45, 211, 2020, 2080, 2704, 2891, 3151
AjuI GAANNNNNNNTTGG 4 cut(s) 1117, 1149, 3083, 3115
Alw21I GWGCWC 7 cut(s) 76, 487, 655, 1901, 1968, 3240, 3355
Alw44I GTGCAC 2 cut(s) 483, 1964
AlwI GGATC 2 cut(s) 1779, 3004
AlwNI CAGNNNCTG 4 cut(s) 82, 1116, 2315, 3323
Ama87I CYCGRG 1 cut(s) 786
Aor13HI TCCGGA 1 cut(s) 2441
AoxI GGCC 8 cut(s) 248, 352, 809, 931, 1191, 1252, 3030, 3053
ApaLI GTGCAC 2 cut(s) 483, 1964
ApeKI GCWGC 8 cut(s) 869, 983, 1349, 1850, 2003, 2219, 2312, 2615
ApoI RAATTY 3 cut(s) 1016, 1131, 2578
ArsI GACNNNNNNTTYG 2 cut(s) 1771, 1803
Asp700I GAANNNNTTC 2 cut(s) 1273, 2571
AspA2I CCTAGG 1 cut(s) 806
AspLEI GCGC 1 cut(s) 3213
AspS9I GGNCC 6 cut(s) 300, 810, 1139, 1178, 3053, 3258
AsuC2I CCSGG 2 cut(s) 1462, 1651
AvaI CYCGRG 1 cut(s) 786
AvaII GGWCC 4 cut(s) 300, 1139, 1178, 3258
AvrII CCTAGG 1 cut(s) 806
BaeGI GKGCMC 4 cut(s) 487, 1527, 1968, 2340
BalI TGGCCA 1 cut(s) 933
BanII GRGCYC 1 cut(s) 3355
BauI CACGAG 1 cut(s) 486
BbsI GAAGAC 4 cut(s) 605, 931, 1853, 3306
Bbv12I GWGCWC 7 cut(s) 76, 487, 655, 1901, 1968, 3240, 3355
BbvI GCAGC 8 cut(s) 881, 995, 1336, 1862, 2015, 2231, 2299, 2602
BceAI ACGGC 5 cut(s) 37, 1178, 1206, 2159, 3017
BciT130I CCWGG 7 cut(s) 47, 213, 2022, 2082, 2706, 2893, 3153
BciVI GTATCC 1 cut(s) 1113
BcnI CCSGG 2 cut(s) 1462, 1651
BfaI CTAG 7 cut(s) 132, 462, 701, 807, 1380, 3087, 3192
BfmI CTRYAG 4 cut(s) 1353, 2616, 2856, 2901
BfuAI ACCTGC 2 cut(s) 2518, 3370
BfuI GTATCC 1 cut(s) 1113
BglII AGATCT 3 cut(s) 7, 3083, 3196
BlnI CCTAGG 1 cut(s) 806
BlpI GCTNAGC 3 cut(s) 75, 1884, 3328
BmcAI AGTACT 1 cut(s) 1579
Bme1390I CCNGG 9 cut(s) 47, 213, 1462, 1651, 2022, 2082, 2706, 2893, 3153
Bme18I GGWCC 4 cut(s) 300, 1139, 1178, 3258
BmeT110I CYCGRG 1 cut(s) 786
BmgT120I GGNCC 6 cut(s) 300, 810, 1139, 1178, 3053, 3258
BmiI GGNNCC 3 cut(s) 302, 2760, 3092
BmrFI CCNGG 9 cut(s) 47, 213, 1462, 1651, 2022, 2082, 2706, 2893, 3153
BmsI GCATC 3 cut(s) 599, 616, 685
BpiI GAAGAC 4 cut(s) 605, 931, 1853, 3306
BpmI CTGGAG 2 cut(s) 549, 2382
Bpu1102I GCTNAGC 3 cut(s) 75, 1884, 3328
BpuEI CTTGAG 6 cut(s) 184, 341, 1166, 2377, 2560, 2663
BpuMI CCSGG 2 cut(s) 1462, 1651
BsaAI YACGTR 1 cut(s) 1725
BsaBI GATNNNNATC 3 cut(s) 840, 1431, 1873
BsaI GGTCTC 1 cut(s) 2392
BsaWI WCCGGW 2 cut(s) 374, 2441
Bsc4I CCNNNNNNNGG 8 cut(s) 940, 1148, 2021, 2750, 2955, 2990, 3067, 3100
Bse1I ACTGG 4 cut(s) 566, 1121, 1386, 2345
Bse3DI GCAATG 2 cut(s) 829, 2616
Bse8I GATNNNNATC 3 cut(s) 840, 1431, 1873
BseAI TCCGGA 1 cut(s) 2441
BseBI CCWGG 7 cut(s) 47, 213, 2022, 2082, 2706, 2893, 3153
BseGI GGATG 8 cut(s) 410, 841, 1642, 1703, 2302, 2438, 2964, 3352
BseJI GATNNNNATC 3 cut(s) 840, 1431, 1873
BseLI CCNNNNNNNGG 8 cut(s) 940, 1148, 2021, 2750, 2955, 2990, 3067, 3100
BseMI GCAATG 2 cut(s) 829, 2616
BseMII CTCAG 7 cut(s) 89, 495, 1875, 2142, 2232, 2315, 3342
BseNI ACTGG 4 cut(s) 566, 1121, 1386, 2345
BseRI GAGGAG 2 cut(s) 2254, 2400
BseSI GKGCMC 4 cut(s) 487, 1527, 1968, 2340
BseX3I CGGCCG 1 cut(s) 1191
BseXI GCAGC 8 cut(s) 881, 995, 1336, 1862, 2015, 2231, 2299, 2602
BseYI CCCAGC 3 cut(s) 656, 1043, 1082
BsgI GTGCAG 2 cut(s) 663, 3348
Bsh1236I CGCG 2 cut(s) 1370, 3183
Bsh1285I CGRYCG 1 cut(s) 1194
BshFI GGCC 8 cut(s) 250, 354, 811, 933, 1193, 1254, 3032, 3055
BsiEI CGRYCG 1 cut(s) 1194
BsiHKAI GWGCWC 7 cut(s) 76, 487, 655, 1901, 1968, 3240, 3355
BsiHKCI CYCGRG 1 cut(s) 786
BsiSI CCGG 5 cut(s) 375, 1462, 1650, 2442, 3220
BslFI GGGAC 3 cut(s) 286, 768, 1164
BslI CCNNNNNNNGG 8 cut(s) 940, 1148, 2021, 2750, 2955, 2990, 3067, 3100
BsmBI CGTCTC 2 cut(s) 2320, 3339
BsmFI GGGAC 3 cut(s) 286, 768, 1164
BsmI GAATGC 1 cut(s) 2971
BsnI GGCC 8 cut(s) 250, 354, 811, 933, 1193, 1254, 3032, 3055
Bso31I GGTCTC 1 cut(s) 2392
BsoBI CYCGRG 1 cut(s) 786
Bsp1286I GDGCHC 9 cut(s) 76, 487, 655, 1527, 1901, 1968, 2340, 3240, 3355
Bsp13I TCCGGA 1 cut(s) 2441
Bsp1407I TGTACA 1 cut(s) 838
Bsp1720I GCTNAGC 3 cut(s) 75, 1884, 3328
Bsp19I CCATGG 4 cut(s) 934, 1181, 1255, 2838
Bsp68I TCGCGA 1 cut(s) 1370
BspACI CCGC 8 cut(s) 247, 298, 1004, 1521, 2642, 2670, 2994, 3181
BspANI GGCC 8 cut(s) 250, 354, 811, 933, 1193, 1254, 3032, 3055
BspCNI CTCAG 7 cut(s) 88, 496, 1876, 2143, 2233, 2316, 3341
BspEI TCCGGA 1 cut(s) 2441
BspFNI CGCG 2 cut(s) 1370, 3183
BspLI GGNNCC 3 cut(s) 302, 2760, 3092
BspMAI CTGCAG 1 cut(s) 2620
BspMI ACCTGC 2 cut(s) 2518, 3370
BspPI GGATC 2 cut(s) 1779, 3004
BspQI GCTCTTC 1 cut(s) 2562
BspTNI GGTCTC 1 cut(s) 2392
BsrDI GCAATG 2 cut(s) 829, 2616
BsrGI TGTACA 1 cut(s) 838
BsrI ACTGG 4 cut(s) 566, 1121, 1386, 2345
BssNAI GTATAC 1 cut(s) 712
BssSI CACGAG 1 cut(s) 486
BssT1I CCWWGG 6 cut(s) 806, 873, 934, 1181, 1255, 2838
Bst1107I GTATAC 1 cut(s) 712
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 7 cut(s) 47, 213, 2022, 2082, 2706, 2893, 3153
Bst6I CTCTTC 2 cut(s) 1928, 2562
BstAPI GCANNNNNTGC 1 cut(s) 2615
BstAUI TGTACA 1 cut(s) 838
BstBAI YACGTR 1 cut(s) 1725
BstC8I GCNNGC 4 cut(s) 352, 867, 1519, 1523
BstDSI CCRYGG 6 cut(s) 934, 1181, 1194, 1255, 2838, 3298
BstEII GGTNACC 1 cut(s) 2944
BstF5I GGATG 8 cut(s) 410, 841, 1642, 1703, 2302, 2438, 2964, 3352
BstFNI CGCG 2 cut(s) 1370, 3183
BstHHI GCGC 1 cut(s) 3213
BstMCI CGRYCG 1 cut(s) 1194
BstMWI GCNNNNNNNGC 3 cut(s) 1679, 2000, 2615
BstNI CCWGG 7 cut(s) 47, 213, 2022, 2082, 2706, 2893, 3153
BstNSI RCATGY 7 cut(s) 587, 869, 1011, 1332, 1479, 1562, 1817
BstPI GGTNACC 1 cut(s) 2944
BstSCI CCNGG 9 cut(s) 45, 211, 1460, 1649, 2020, 2080, 2704, 2891, 3151
BstSFI CTRYAG 4 cut(s) 1353, 2616, 2856, 2901
BstSLI GKGCMC 4 cut(s) 487, 1527, 1968, 2340
BstUI CGCG 2 cut(s) 1370, 3183
BstV1I GCAGC 8 cut(s) 881, 995, 1336, 1862, 2015, 2231, 2299, 2602
BstV2I GAAGAC 4 cut(s) 605, 931, 1853, 3306
BstX2I RGATCY 3 cut(s) 7, 3083, 3196
BstXI CCANNNNNNTGG 1 cut(s) 2959
BstYI RGATCY 3 cut(s) 7, 3083, 3196
BstZ17I GTATAC 1 cut(s) 712
BstZI CGGCCG 1 cut(s) 1191
BsuI GTATCC 1 cut(s) 1113
BsuRI GGCC 8 cut(s) 250, 354, 811, 933, 1193, 1254, 3032, 3055
BtgI CCRYGG 6 cut(s) 934, 1181, 1194, 1255, 2838, 3298
BtgZI GCGATG 2 cut(s) 1245, 3198
BtsCI GGATG 8 cut(s) 410, 841, 1642, 1703, 2302, 2438, 2964, 3352
BtsI GCAGTG 2 cut(s) 1344, 1966
BtsIMutI CAGTG 9 cut(s) 745, 1101, 1114, 1344, 1901, 1966, 2006, 2340, 3033
BtuMI TCGCGA 1 cut(s) 1370
BveI ACCTGC 2 cut(s) 2518, 3370
Cac8I GCNNGC 4 cut(s) 352, 867, 1519, 1523
CaiI CAGNNNCTG 4 cut(s) 82, 1116, 2315, 3323
CfoI GCGC 1 cut(s) 3213
Cfr13I GGNCC 6 cut(s) 300, 810, 1139, 1178, 3053, 3258
CseI GACGC 1 cut(s) 3321
CsiI ACCWGGT 1 cut(s) 2080
Csp6I GTAC 9 cut(s) 378, 424, 839, 1071, 1578, 2177, 3078, 3280, 3289
CspCI CAANNNNNGTGG 2 cut(s) 3280, 3315
CviQI GTAC 9 cut(s) 378, 424, 839, 1071, 1578, 2177, 3078, 3280, 3289
DraI TTTAAA 2 cut(s) 523, 1803
EaeI YGGCCR 3 cut(s) 931, 1191, 3030
EagI CGGCCG 1 cut(s) 1191
Eam1104I CTCTTC 2 cut(s) 1928, 2562
EarI CTCTTC 2 cut(s) 1928, 2562
EciI GGCGGA 1 cut(s) 3009
Ecl136II GAGCTC 1 cut(s) 3353
EclXI CGGCCG 1 cut(s) 1191
Eco130I CCWWGG 6 cut(s) 806, 873, 934, 1181, 1255, 2838
Eco24I GRGCYC 1 cut(s) 3355
Eco31I GGTCTC 1 cut(s) 2392
Eco32I GATATC 1 cut(s) 242
Eco47I GGWCC 4 cut(s) 300, 1139, 1178, 3258
Eco52I CGGCCG 1 cut(s) 1191
Eco53kI GAGCTC 1 cut(s) 3353
Eco57I CTGAAG 3 cut(s) 2558, 3199, 3300
Eco88I CYCGRG 1 cut(s) 786
Eco91I GGTNACC 1 cut(s) 2944
EcoICRI GAGCTC 1 cut(s) 3353
EcoO109I RGGNCCY 2 cut(s) 810, 1139
EcoO65I GGTNACC 1 cut(s) 2944
EcoRI GAATTC 1 cut(s) 2578
EcoRII CCWGG 7 cut(s) 45, 211, 2020, 2080, 2704, 2891, 3151
EcoRV GATATC 1 cut(s) 242
EcoT14I CCWWGG 6 cut(s) 806, 873, 934, 1181, 1255, 2838
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 3355
ErhI CCWWGG 6 cut(s) 806, 873, 934, 1181, 1255, 2838
Esp3I CGTCTC 2 cut(s) 2320, 3339
FalI AAGNNNNNCTT 4 cut(s) 1449, 1481, 2556, 2588
FaqI GGGAC 3 cut(s) 286, 768, 1164
FauI CCCGC 1 cut(s) 1514
FauNDI CATATG 1 cut(s) 716
FblI GTMKAC 2 cut(s) 711, 1909
FokI GGATG 8 cut(s) 417, 848, 1649, 1690, 2309, 2425, 2971, 3359
FriOI GRGCYC 1 cut(s) 3355
FspBI CTAG 7 cut(s) 132, 462, 701, 807, 1380, 3087, 3192
GlaI GCGC 1 cut(s) 3212
GsaI CCCAGC 3 cut(s) 660, 1047, 1086
GsuI CTGGAG 2 cut(s) 549, 2382
HaeIII GGCC 8 cut(s) 250, 354, 811, 933, 1193, 1254, 3032, 3055
HapII CCGG 5 cut(s) 375, 1462, 1650, 2442, 3220
HgaI GACGC 1 cut(s) 3321
HhaI GCGC 1 cut(s) 3213
Hin6I GCGC 1 cut(s) 3211
HinP1I GCGC 1 cut(s) 3211
HpaII CCGG 5 cut(s) 375, 1462, 1650, 2442, 3220
HpyAV CCTTC 6 cut(s) 61, 1790, 2190, 2286, 2461, 2728
HpyCH4IV ACGT 4 cut(s) 1724, 2187, 2521, 3294
HpyF10VI GCNNNNNNNGC 3 cut(s) 1679, 2000, 2615
HpySE526I ACGT 4 cut(s) 1724, 2187, 2521, 3294
HspAI GCGC 1 cut(s) 3211
Kpn2I TCCGGA 1 cut(s) 2441
LguI GCTCTTC 1 cut(s) 2562
LmnI GCTCC 8 cut(s) 395, 658, 755, 1048, 2267, 2758, 2938, 3350
Lsp1109I GCAGC 8 cut(s) 881, 995, 1336, 1862, 2015, 2231, 2299, 2602
LweI GCATC 3 cut(s) 599, 616, 685
MabI ACCWGGT 1 cut(s) 2080
MaeI CTAG 7 cut(s) 132, 462, 701, 807, 1380, 3087, 3192
MaeII ACGT 4 cut(s) 1724, 2187, 2521, 3294
MflI RGATCY 3 cut(s) 7, 3083, 3196
MhlI GDGCHC 9 cut(s) 76, 487, 655, 1527, 1901, 1968, 2340, 3240, 3355
MlsI TGGCCA 1 cut(s) 933
MluNI TGGCCA 1 cut(s) 933
MlyI GAGTC 4 cut(s) 120, 2843, 2875, 3041
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 933
Mph1103I ATGCAT 1 cut(s) 631
MroI TCCGGA 1 cut(s) 2441
MroXI GAANNNNTTC 2 cut(s) 1273, 2571
MscI TGGCCA 1 cut(s) 933
MseI TTAA 9 cut(s) 522, 747, 1467, 1532, 1700, 1802, 2291, 2828, 2862
MslI CAYNNNNRTG 2 cut(s) 1026, 1761
Msp20I TGGCCA 1 cut(s) 933
MspA1I CMGCKG 1 cut(s) 2312
MspI CCGG 5 cut(s) 375, 1462, 1650, 2442, 3220
MspR9I CCNGG 9 cut(s) 47, 213, 1462, 1651, 2022, 2082, 2706, 2893, 3153
Mva1269I GAATGC 1 cut(s) 2971
MvaI CCWGG 7 cut(s) 47, 213, 2022, 2082, 2706, 2893, 3153
MvnI CGCG 2 cut(s) 1370, 3183
MwoI GCNNNNNNNGC 3 cut(s) 1679, 2000, 2615
NciI CCSGG 2 cut(s) 1462, 1651
NcoI CCATGG 4 cut(s) 934, 1181, 1255, 2838
NdeI CATATG 1 cut(s) 716
NlaIV GGNNCC 3 cut(s) 302, 2760, 3092
NruI TCGCGA 1 cut(s) 1370
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 7 cut(s) 587, 869, 1011, 1332, 1479, 1562, 1817
PaeI GCATGC 1 cut(s) 869
PaqCI CACCTGC 1 cut(s) 3370
PasI CCCWGGG 1 cut(s) 2892
PciI ACATGT 2 cut(s) 1475, 1813
PciSI GCTCTTC 1 cut(s) 2562
PctI GAATGC 1 cut(s) 2971
PdmI GAANNNNTTC 2 cut(s) 1273, 2571
PfeI GAWTC 8 cut(s) 140, 914, 974, 1295, 1600, 2045, 2624, 2897
PflMI CCANNNNNTGG 1 cut(s) 940
PfoI TCCNGGA 2 cut(s) 211, 2020
PleI GAGTC 4 cut(s) 120, 2843, 2875, 3041
PpsI GAGTC 4 cut(s) 120, 2843, 2875, 3041
Ppu21I YACGTR 1 cut(s) 1725
PpuMI RGGWCCY 1 cut(s) 1139
PscI ACATGT 2 cut(s) 1475, 1813
PsiI TTATAA 1 cut(s) 2657
Psp124BI GAGCTC 1 cut(s) 3355
Psp5II RGGWCCY 1 cut(s) 1139
Psp6I CCWGG 7 cut(s) 45, 211, 2020, 2080, 2704, 2891, 3151
PspEI GGTNACC 1 cut(s) 2944
PspFI CCCAGC 3 cut(s) 656, 1043, 1082
PspGI CCWGG 7 cut(s) 45, 211, 2020, 2080, 2704, 2891, 3151
PspN4I GGNNCC 3 cut(s) 302, 2760, 3092
PspPI GGNCC 6 cut(s) 300, 810, 1139, 1178, 3053, 3258
PspPPI RGGWCCY 1 cut(s) 1139
PstI CTGCAG 1 cut(s) 2620
PstNI CAGNNNCTG 4 cut(s) 82, 1116, 2315, 3323
PsuI RGATCY 3 cut(s) 7, 3083, 3196
PvuII CAGCTG 1 cut(s) 2312
RruI TCGCGA 1 cut(s) 1370
RsaI GTAC 9 cut(s) 379, 425, 840, 1072, 1579, 2178, 3079, 3281, 3290
RsaNI GTAC 9 cut(s) 378, 424, 839, 1071, 1578, 2177, 3078, 3280, 3289
RseI CAYNNNNRTG 2 cut(s) 1026, 1761
SacI GAGCTC 1 cut(s) 3355
SapI GCTCTTC 1 cut(s) 2562
SaqAI TTAA 9 cut(s) 522, 747, 1467, 1532, 1700, 1802, 2291, 2828, 2862
Sau96I GGNCC 6 cut(s) 300, 810, 1139, 1178, 3053, 3258
ScaI AGTACT 1 cut(s) 1579
SchI GAGTC 4 cut(s) 120, 2843, 2875, 3041
ScrFI CCNGG 9 cut(s) 47, 213, 1462, 1651, 2022, 2082, 2706, 2893, 3153
SduI GDGCHC 9 cut(s) 76, 487, 655, 1527, 1901, 1968, 2340, 3240, 3355
SexAI ACCWGGT 1 cut(s) 2080
SfaNI GCATC 3 cut(s) 599, 616, 685
SfcI CTRYAG 4 cut(s) 1353, 2616, 2856, 2901
SinI GGWCC 4 cut(s) 300, 1139, 1178, 3258
SmiMI CAYNNNNRTG 2 cut(s) 1026, 1761
SmlI CTYRAG 6 cut(s) 199, 356, 1145, 2392, 2539, 2678
SmoI CTYRAG 6 cut(s) 199, 356, 1145, 2392, 2539, 2678
SphI GCATGC 1 cut(s) 869
SsiI CCGC 8 cut(s) 247, 298, 1004, 1521, 2642, 2670, 2994, 3181
SspMI CTAG 7 cut(s) 132, 462, 701, 807, 1380, 3087, 3192
SstI GAGCTC 1 cut(s) 3355
StyD4I CCNGG 9 cut(s) 45, 211, 1460, 1649, 2020, 2080, 2704, 2891, 3151
StyI CCWWGG 6 cut(s) 806, 873, 934, 1181, 1255, 2838
TaiI ACGT 4 cut(s) 1727, 2190, 2524, 3297
TaqI TCGA 7 cut(s) 124, 161, 634, 1774, 2035, 2372, 2872
TatI WGTACW 3 cut(s) 838, 1577, 3288
TauI GCSGC 3 cut(s) 250, 1007, 2645
TfiI GAWTC 8 cut(s) 140, 914, 974, 1295, 1600, 2045, 2624, 2897
Tru1I TTAA 9 cut(s) 522, 747, 1467, 1532, 1700, 1802, 2291, 2828, 2862
Tru9I TTAA 9 cut(s) 522, 747, 1467, 1532, 1700, 1802, 2291, 2828, 2862
TscAI CASTG 9 cut(s) 745, 1108, 1121, 1351, 1901, 1973, 2013, 2340, 3033
TseI GCWGC 8 cut(s) 869, 983, 1349, 1850, 2003, 2219, 2312, 2615
TspDTI ATGAA 8 cut(s) 1010, 1266, 1383, 1496, 1728, 1965, 2762, 2928
TspGWI ACGGA 4 cut(s) 108, 306, 1437, 3287
TspRI CASTG 9 cut(s) 745, 1108, 1121, 1351, 1901, 1973, 2013, 2340, 3033
Van91I CCANNNNNTGG 1 cut(s) 940
VneI GTGCAC 2 cut(s) 483, 1964
VpaK11BI GGWCC 4 cut(s) 300, 1139, 1178, 3258
XapI RAATTY 3 cut(s) 1016, 1131, 2578
XceI RCATGY 7 cut(s) 587, 869, 1011, 1332, 1479, 1562, 1817
XmaJI CCTAGG 1 cut(s) 806
XmiI GTMKAC 2 cut(s) 711, 1909
XmnI GAANNNNTTC 2 cut(s) 1273, 2571
XspI CTAG 7 cut(s) 132, 462, 701, 807, 1380, 3087, 3192
ZrmI AGTACT 1 cut(s) 1579
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.