Rh6DG209400

Pleckstrin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009215
Physical Location & Seq
Reverse (-)
37321857 .. 37328067
6211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG209400.1

Sequence Viewer

Length: 3300 bp
ATGGCAGATCTTGCTAGCTACGGGAATGCCAACCGTGACATTGACCAGGACGAATCATCTTTGATCTGGATTTCGAGTAGTGGAGAAAGAACTTTGAAGTTAGCTTCTGTCTCAAGAATTATTCCTGGGCAAAGAACTGCCGTTTTCCAACGATATCTGCGTCCTGAAAAGGATTATTTATCTTTTTCTCTTATATATAATAACGGAAAGCGTTCCCTTGATCTGATTTGCAAGGATAAAGTCGAGGCAGAAGTGTGGATTGCTGGCCTCAAAGCACTTATATCCTCTGGTAGAGGTGGCCGCTCCAAAATTGATGGATGGAGTGATGGAGGCTTGTACCTTGATGATAGCAGAGACTTGACATCAAATAGCCCTAGTGATAGTTCAGTTAGTGGTACACGAGATATTGGGTCTCCTGAGATTTCTGTCAGTTTCAAACCAAATACTTCACCAAAGAGTTTTCCGCCTGACAATTCTCCGGTCTCTGAGAGGTCACATGTAGCATCAGACCAGACAAATATGCAAGTAAAAGGATCCGGTTCAGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCCAGCACTTCAAGTCATGGTTCTGCACCCGATGATTGTGATGCTCTAGGTGATGTATACATATGGGGTGAGGTCATTTGTGATAGTGTCGTTAAGATTGGAGCTGATAAAAATGTAAATTATTTGAGTCCCCGAGCAGATGTGCTTGTTCCTAGGCCATTAGAGTCCAATGTTGTTTTGGATGTACATCATATAGCCTGTGGGGTCAAGCATGCAGCCTTGGTCACAAGACAAGGTGAAGTTTTTACATGGGGTGAAGAATCTGGGGGACGACTTGGCCATGGTGTGGGGAAGGATGTTGCTCAACCCCGTCTAGTTGAATCTCTAGCAGCTACTAGTGTTGATTTTGCGGCGTGTGGAGAATTTCATAGTTGTGCTGTTACAATGGCTGGGGAACTTTATACGTGGGGAGATGGTACACACAATGCTGGGCTTCTTGGTCATGGCACTGATGTCAGTCACTGGATACCAAAGAGAATTTCAGGTCCTCTTGAGGGACTTCAAGTTGCTTCTGTATCTTGTGGTCCATGGCATACGGCCTTGGTGACATCAACGGGGAAACTTTTTACATTTGGTGATGGAACATTTGGTGTCTTAGGCCATGGAGACAGAGAAAATGTTTCATATCCGAGAGAAGTAGATTCCCTAACAGGTTTGAGGACAATTGCTGTTGCATGTGGGGTATGGCACACTGCTGCTGTAGTAGAGGTTATTGCAACACAATCTAGTGCCAGTATTTCATCAGGTAAATTGTTTACTTGGGGTGATGGAGATAAAAATCGTCTTGGACATGGAGACAAGGAAGCCCGGCTTAAACCGACGTGTGTTCCAGCCCTTATTGATTACAATTTTCACAAAATTGCTTGCGGGCACAGTTTAACTGTTGGCTTGACAACATCAGGGCATGTCTTTACAATGGGTAGTACTGTTTATGGTCAGCTCGGAAATCCCAATTCTGATGGAAAAATACCTTGCTTAGTGGATGACAAACTTTCCGGGGAATGTATTGAAGAAATTGCTTGTGGTGCATATCATGTAGCAGTATTAACATCCAGAAATGAAGTTTATACATGGGGAAAAGGTGCTAATGGGAGGTTGGGGCATGGTGATGTTGAGGATCGAAAAACACCGACTATGGTCGAAGGTTTGAAGGATAGACATGTGAAATATATTGGTTGTGGTTCACACTATACAGCAGCTATATGTCTTCACAAATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGATTTACAAGGAAGAGGCATAATTGCTATAATTGTGGACTCGTGCACTGCCATTCATGTAGTTCCAGAAAAGCAACAAGAGCAGCACTGTCTCCTAATCCTGGAAAACCGTATCGAGTGTGTGATTCTTGTTATGTGAAATTGAACAAGGTGTTAGAACCTGGTAGTAATAATAGAAAGAATGTTATACCTCGCTTGTCTGGTGAGAACAAGGACAGATTGGACAAGGCTGACATAAGATTATACAAGTCTGCTGTACCTTCAAACGTGGATTTGATAAAGCAATTAGATAGCAAAGCAGCCAAGCAAGGAAAGAAAGCTGAGACATTTTCTCTTGTTCGCTCCTCTCAAGCACCTTCGCTGTTGCAATTAAAGGATGTAGTTATGTCAGCTGCTGTTGATCTGAGACGAACAGTTCCCAAACCAGTTCTTACACCATCTGGAGTAAGTTCGAGGTCTGTGTCGCCTTTCTCAAGGAGACCAAGCCCTCCTCGTTTTGCAACTCCTGTTCCTACAACATCTGGACTTTCCTTCTCAAAAAGTATCGCAGATAGTTTGAAGAAAACTAATGAGCTTTTGAATCAGGAAGTGCTAAAGTTACGTTCACAGGTTGATAGCCTGAGACAGAGATGTGAACTTCAAGAAGTAGAGCTTCAGAATTCAACAAAGAAAGCTCAAGAAGCTATGACAGTGGCTGCAGAGGAATCTGCGAAATCTAAAGCGGCTAAAGAAGTTATAAAGTCACTAACTGCACAGCTCAAGGATTTGGCTGAAAGGCTGCCACCTGGGGTTTATGATACTGAGGGCATAAAGCAGGCATATCCATCTTATGGTTTGGAGTCAAATGGCATTCACTATCCAGATGGAGATAACCATTCAAGATCAAGTTCAATGAGCAATTCTTACTTAATTTCCTCCATGGGAATCGACTCAACTACAGTAAACGGAAGTCTAGGGCAGACTCATTCACCCAGGGATTCAGTAGGAACGAATGAAACTAACGTGCAACACAATAGGGAGCTGGTGACCTCCAATGGGATGGTAAATGCACTAGATAGACTGCCCAATGGTGGCGGATCATTTCAGTCAGTTGGTAGTAATCTGTCGGTGGCTGTTGATGGCAAGGACTCTGGGCCTTTTCAAGATGGGGAGAATGGTACGAGATCTAGGAATTCTGCATTGGCTGTTACTGGTAATACAGTTGAGGCAGAATGGATTGAACAATATGAGCCTGGTGTTTACATAACTCTTGTGGCTCTTCGTGATGGAACTAGAGATCTCAAACGAGTGCGCTTCAGCCGGAGAAGATTTGGAGAACACCAAGCAGAGATTTGGTGGTCTGAGAATCGCGAAAAAGTGTACGAGAAGTACAACGTCCGTGGATCGGACAAGTCTTCAGTTTCTGGCTCAGCAGCGCGTAGATCGGATGGAGCTCTCTCACCTGCATCCCAACAACCCTAG

Protein Analysis

1099

Amino Acids

118.32

Weight (kDa)

8.34

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 29 - 94 1.7e-06 Pleckstrin homology domain
WD40_RLD PF25390 210 - 591 4e-77 RCC1-like domain
RCC1 PF00415 269 - 318 6.8e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 322 - 373 5.2e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 360 - 388 1.6e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 377 - 425 1.4e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 439 - 487 8.1e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 491 - 540 6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 528 - 555 5.4e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 545 - 592 8.4e-14 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 596 - 662 4.6e-15 FYVE zinc finger
BRX_N PF13713 847 - 881 4.9e-18 Transcription factor BRX N-terminal domain
BRX PF08381 1015 - 1070 7.5e-29 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2576
AarI CACCTGC 1 cut(s) 3289
Acc36I ACCTGC 1 cut(s) 3289
AccB7I CCANNNNNTGG 2 cut(s) 859, 2669
AccBSI CCGCTC 1 cut(s) 303
AccI GTMKAC 2 cut(s) 630, 1828
AccII CGCG 2 cut(s) 3189, 3256
AciI CCGC 6 cut(s) 301, 464, 923, 1440, 2561, 2913
AclWI GGATC 5 cut(s) 528, 541, 1698, 2923, 3229
AcoI YGGCCR 2 cut(s) 298, 850
AcsI RAATTY 4 cut(s) 935, 1050, 2497, 3010
AcuI CTGAAG 3 cut(s) 2477, 3118, 3219
AfaI GTAC 9 cut(s) 338, 397, 759, 991, 1498, 2097, 2998, 3200, 3209
AfiI CCNNNNNNNGG 7 cut(s) 859, 1067, 1940, 2669, 2874, 2909, 3223
AflIII ACRYGT 3 cut(s) 496, 1394, 1732
AhlI ACTAGT 1 cut(s) 908
AjiI CACGTC 1 cut(s) 1395
AjnI CCWGG 7 cut(s) 45, 124, 1939, 1999, 2623, 2810, 3070
AjuI GAANNNNNNNTTGG 4 cut(s) 1036, 1068, 3002, 3034
Alw21I GWGCWC 4 cut(s) 574, 1820, 1887, 3274
Alw44I GTGCAC 1 cut(s) 1883
AlwI GGATC 5 cut(s) 528, 541, 1698, 2923, 3229
AlwNI CAGNNNCTG 4 cut(s) 1035, 2234, 2534, 3242
Ama87I CYCGRG 1 cut(s) 705
AoxI GGCC 7 cut(s) 265, 298, 728, 850, 1110, 1171, 2972
ApaLI GTGCAC 1 cut(s) 1883
ApoI RAATTY 4 cut(s) 935, 1050, 2497, 3010
Asp700I GAANNNNTTC 1 cut(s) 211
AspA2I CCTAGG 1 cut(s) 725
AspLEI GCGC 2 cut(s) 3132, 3256
AspS9I GGNCC 3 cut(s) 1058, 1097, 2972
AsuC2I CCSGG 2 cut(s) 1381, 1570
AsuNHI GCTAGC 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 2 cut(s) 1058, 1097
AvrII CCTAGG 1 cut(s) 725
BaeGI GKGCMC 2 cut(s) 1446, 1887
BalI TGGCCA 1 cut(s) 852
BamHI GGATCC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 3274
BauI CACGAG 2 cut(s) 399, 1880
BbsI GAAGAC 2 cut(s) 1772, 3225
Bbv12I GWGCWC 4 cut(s) 574, 1820, 1887, 3274
BceAI ACGGC 2 cut(s) 125, 1125
BciT130I CCWGG 7 cut(s) 47, 126, 1941, 2001, 2625, 2812, 3072
BciVI GTATCC 1 cut(s) 1032
BcnI CCSGG 2 cut(s) 1381, 1570
BcuI ACTAGT 1 cut(s) 908
BfmI CTRYAG 3 cut(s) 1272, 2535, 2775
BfuAI ACCTGC 1 cut(s) 3289
BfuI GTATCC 1 cut(s) 1032
BglII AGATCT 3 cut(s) 7, 3002, 3115
BlnI CCTAGG 1 cut(s) 725
BlpI GCTNAGC 2 cut(s) 1803, 3247
BmcAI AGTACT 1 cut(s) 1498
Bme1390I CCNGG 9 cut(s) 47, 126, 1381, 1570, 1941, 2001, 2625, 2812, 3072
Bme18I GGWCC 2 cut(s) 1058, 1097
BmeT110I CYCGRG 1 cut(s) 705
BmgBI CACGTC 1 cut(s) 1395
BmgT120I GGNCC 3 cut(s) 1058, 1097, 2972
BmiI GGNNCC 1 cut(s) 535
BmrFI CCNGG 9 cut(s) 47, 126, 1381, 1570, 1941, 2001, 2625, 2812, 3072
BmsI GCATC 4 cut(s) 512, 535, 604, 3293
BmtI GCTAGC 1 cut(s) 18
BoxI GACNNNNGTC 1 cut(s) 1709
BpiI GAAGAC 2 cut(s) 1772, 3225
BpmI CTGGAG 1 cut(s) 2301
Bpu1102I GCTNAGC 2 cut(s) 1803, 3247
BpuEI CTTGAG 6 cut(s) 97, 1085, 2172, 2296, 2499, 2582
BpuMI CCSGG 2 cut(s) 1381, 1570
BsaAI YACGTR 1 cut(s) 978
BsaBI GATNNNNATC 2 cut(s) 759, 1350
BsaI GGTCTC 3 cut(s) 417, 487, 2311
BsaWI WCCGGW 2 cut(s) 478, 536
Bsc4I CCNNNNNNNGG 7 cut(s) 859, 1067, 1940, 2669, 2874, 2909, 3223
Bse1I ACTGG 4 cut(s) 1040, 1305, 2264, 3034
Bse8I GATNNNNATC 2 cut(s) 759, 1350
BseBI CCWGG 7 cut(s) 47, 126, 1941, 2001, 2625, 2812, 3072
BseGI GGATG 9 cut(s) 323, 760, 874, 1561, 1622, 2221, 2883, 3271, 3284
BseJI GATNNNNATC 2 cut(s) 759, 1350
BseLI CCNNNNNNNGG 7 cut(s) 859, 1067, 1940, 2669, 2874, 2909, 3223
BseMII CTCAG 9 cut(s) 408, 477, 1794, 2151, 2234, 2450, 2631, 3171, 3261
BseNI ACTGG 4 cut(s) 1040, 1305, 2264, 3034
BseRI GAGGAG 2 cut(s) 2173, 2319
BseSI GKGCMC 2 cut(s) 1446, 1887
BseYI CCCAGC 3 cut(s) 575, 962, 1001
BsgI GTGCAG 2 cut(s) 582, 2574
Bsh1236I CGCG 2 cut(s) 3189, 3256
BshFI GGCC 7 cut(s) 267, 300, 730, 852, 1112, 1173, 2974
BsiHKAI GWGCWC 4 cut(s) 574, 1820, 1887, 3274
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 5 cut(s) 479, 537, 1381, 1569, 3139
BslFI GGGAC 3 cut(s) 687, 855, 1083
BslI CCNNNNNNNGG 7 cut(s) 859, 1067, 1940, 2669, 2874, 2909, 3223
BsmBI CGTCTC 1 cut(s) 2239
BsmFI GGGAC 3 cut(s) 687, 855, 1083
BsmI GAATGC 2 cut(s) 31, 2688
BsnI GGCC 7 cut(s) 267, 300, 730, 852, 1112, 1173, 2974
Bso31I GGTCTC 3 cut(s) 417, 487, 2311
BsoBI CYCGRG 1 cut(s) 705
Bsp1286I GDGCHC 5 cut(s) 574, 1446, 1820, 1887, 3274
Bsp1407I TGTACA 1 cut(s) 757
Bsp1720I GCTNAGC 2 cut(s) 1803, 3247
Bsp19I CCATGG 4 cut(s) 853, 1100, 1174, 2757
Bsp68I TCGCGA 1 cut(s) 3189
BspACI CCGC 6 cut(s) 301, 464, 923, 1440, 2561, 2913
BspANI GGCC 7 cut(s) 267, 300, 730, 852, 1112, 1173, 2974
BspCNI CTCAG 9 cut(s) 409, 478, 1795, 2152, 2235, 2451, 2632, 3172, 3260
BspFNI CGCG 2 cut(s) 3189, 3256
BspLI GGNNCC 1 cut(s) 535
BspMAI CTGCAG 1 cut(s) 2539
BspMI ACCTGC 1 cut(s) 3289
BspOI GCTAGC 1 cut(s) 18
BspPI GGATC 5 cut(s) 528, 541, 1698, 2923, 3229
BspQI GCTCTTC 1 cut(s) 3102
BspTNI GGTCTC 3 cut(s) 417, 487, 2311
BsrBI CCGCTC 1 cut(s) 303
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 4 cut(s) 1040, 1305, 2264, 3034
BssNAI GTATAC 1 cut(s) 631
BssSI CACGAG 2 cut(s) 399, 1880
BssT1I CCWWGG 7 cut(s) 725, 792, 853, 1100, 1113, 1174, 2757
Bst1107I GTATAC 1 cut(s) 631
Bst2BI CACGAG 2 cut(s) 399, 1880
Bst2UI CCWGG 7 cut(s) 47, 126, 1941, 2001, 2625, 2812, 3072
Bst6I CTCTTC 2 cut(s) 1847, 3102
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 757
BstBAI YACGTR 1 cut(s) 978
BstC8I GCNNGC 6 cut(s) 16, 265, 786, 1438, 1442, 2655
BstDSI CCRYGG 5 cut(s) 853, 1100, 1174, 2757, 3217
BstEII GGTNACC 1 cut(s) 2863
BstF5I GGATG 9 cut(s) 323, 760, 874, 1561, 1622, 2221, 2883, 3271, 3284
BstFNI CGCG 2 cut(s) 3189, 3256
BstHHI GCGC 2 cut(s) 3132, 3256
BstMWI GCNNNNNNNGC 4 cut(s) 11, 1598, 1919, 2519
BstNI CCWGG 7 cut(s) 47, 126, 1941, 2001, 2625, 2812, 3072
BstNSI RCATGY 5 cut(s) 500, 788, 1251, 1481, 1736
BstPAI GACNNNNGTC 1 cut(s) 1709
BstPI GGTNACC 1 cut(s) 2863
BstSCI CCNGG 9 cut(s) 45, 124, 1379, 1568, 1939, 1999, 2623, 2810, 3070
BstSFI CTRYAG 3 cut(s) 1272, 2535, 2775
BstSLI GKGCMC 2 cut(s) 1446, 1887
BstUI CGCG 2 cut(s) 3189, 3256
BstV2I GAAGAC 2 cut(s) 1772, 3225
BstX2I RGATCY 4 cut(s) 7, 533, 3002, 3115
BstXI CCANNNNNNTGG 1 cut(s) 2878
BstYI RGATCY 4 cut(s) 7, 533, 3002, 3115
BstZ17I GTATAC 1 cut(s) 631
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 7 cut(s) 267, 300, 730, 852, 1112, 1173, 2974
BtgI CCRYGG 5 cut(s) 853, 1100, 1174, 2757, 3217
BtrI CACGTC 1 cut(s) 1395
BtsCI GGATG 9 cut(s) 323, 760, 874, 1561, 1622, 2221, 2883, 3271, 3284
BtsI GCAGTG 2 cut(s) 1263, 1885
BtsIMutI CAGTG 7 cut(s) 1020, 1033, 1263, 1820, 1885, 1925, 2535
BtuMI TCGCGA 1 cut(s) 3189
BveI ACCTGC 1 cut(s) 3289
Cac8I GCNNGC 6 cut(s) 16, 265, 786, 1438, 1442, 2655
CaiI CAGNNNCTG 4 cut(s) 1035, 2234, 2534, 3242
CfoI GCGC 2 cut(s) 3132, 3256
Cfr13I GGNCC 3 cut(s) 1058, 1097, 2972
CseI GACGC 1 cut(s) 149
CsiI ACCWGGT 1 cut(s) 1999
Csp6I GTAC 9 cut(s) 337, 396, 758, 990, 1497, 2096, 2997, 3199, 3208
CspCI CAANNNNNGTGG 2 cut(s) 3199, 3234
CviQI GTAC 9 cut(s) 337, 396, 758, 990, 1497, 2096, 2997, 3199, 3208
EaeI YGGCCR 2 cut(s) 298, 850
Eam1104I CTCTTC 2 cut(s) 1847, 3102
EarI CTCTTC 2 cut(s) 1847, 3102
EciI GGCGGA 2 cut(s) 453, 2928
Ecl136II GAGCTC 1 cut(s) 3272
Eco130I CCWWGG 7 cut(s) 725, 792, 853, 1100, 1113, 1174, 2757
Eco24I GRGCYC 1 cut(s) 3274
Eco31I GGTCTC 3 cut(s) 417, 487, 2311
Eco32I GATATC 1 cut(s) 155
Eco47I GGWCC 2 cut(s) 1058, 1097
Eco53kI GAGCTC 1 cut(s) 3272
Eco57I CTGAAG 3 cut(s) 2477, 3118, 3219
Eco88I CYCGRG 1 cut(s) 705
Eco91I GGTNACC 1 cut(s) 2863
EcoICRI GAGCTC 1 cut(s) 3272
EcoO109I RGGNCCY 1 cut(s) 1058
EcoO65I GGTNACC 1 cut(s) 2863
EcoRI GAATTC 2 cut(s) 2497, 3010
EcoRII CCWGG 7 cut(s) 45, 124, 1939, 1999, 2623, 2810, 3070
EcoRV GATATC 1 cut(s) 155
EcoT14I CCWWGG 7 cut(s) 725, 792, 853, 1100, 1113, 1174, 2757
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 3274
ErhI CCWWGG 7 cut(s) 725, 792, 853, 1100, 1113, 1174, 2757
Esp3I CGTCTC 1 cut(s) 2239
FalI AAGNNNNNCTT 4 cut(s) 1368, 1400, 2475, 2507
FaqI GGGAC 3 cut(s) 687, 855, 1083
FauI CCCGC 1 cut(s) 1433
FauNDI CATATG 1 cut(s) 635
FblI GTMKAC 2 cut(s) 630, 1828
FokI GGATG 9 cut(s) 330, 767, 881, 1568, 1609, 2228, 2890, 3271, 3278
FriOI GRGCYC 1 cut(s) 3274
GlaI GCGC 2 cut(s) 3131, 3255
GsaI CCCAGC 3 cut(s) 579, 966, 1005
GsuI CTGGAG 1 cut(s) 2301
HaeIII GGCC 7 cut(s) 267, 300, 730, 852, 1112, 1173, 2974
HapII CCGG 5 cut(s) 479, 537, 1381, 1569, 3139
HgaI GACGC 1 cut(s) 149
HhaI GCGC 2 cut(s) 3132, 3256
Hin6I GCGC 2 cut(s) 3130, 3254
HinP1I GCGC 2 cut(s) 3130, 3254
HpaII CCGG 5 cut(s) 479, 537, 1381, 1569, 3139
Hpy99I CGWCG 1 cut(s) 1396
HpyAV CCTTC 6 cut(s) 859, 1709, 1717, 2109, 2205, 2380
HpyCH4IV ACGT 6 cut(s) 977, 1394, 2106, 2440, 2841, 3213
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 1598, 1919, 2519
HpySE526I ACGT 6 cut(s) 977, 1394, 2106, 2440, 2841, 3213
HspAI GCGC 2 cut(s) 3130, 3254
LguI GCTCTTC 1 cut(s) 3102
LmnI GCTCC 6 cut(s) 308, 577, 674, 2186, 2857, 3269
LweI GCATC 4 cut(s) 512, 535, 604, 3293
MabI ACCWGGT 1 cut(s) 1999
MaeII ACGT 6 cut(s) 977, 1394, 2106, 2440, 2841, 3213
MbiI CCGCTC 1 cut(s) 303
MboII GAAGA 8 cut(s) 842, 1595, 1772, 1864, 2410, 3089, 3156, 3225
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 4 cut(s) 7, 533, 3002, 3115
MhlI GDGCHC 5 cut(s) 574, 1446, 1820, 1887, 3274
MlsI TGGCCA 1 cut(s) 852
MluNI TGGCCA 1 cut(s) 852
MlyI GAGTC 7 cut(s) 709, 746, 1872, 2687, 2762, 2794, 2960
MmeI TCCRAC 1 cut(s) 172
Mox20I TGGCCA 1 cut(s) 852
Mph1103I ATGCAT 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 211
MscI TGGCCA 1 cut(s) 852
MseI TTAA 6 cut(s) 666, 1386, 1451, 1619, 2210, 2747
MslI CAYNNNNRTG 2 cut(s) 945, 1680
Msp20I TGGCCA 1 cut(s) 852
MspA1I CMGCKG 1 cut(s) 2231
MspI CCGG 5 cut(s) 479, 537, 1381, 1569, 3139
MspR9I CCNGG 9 cut(s) 47, 126, 1381, 1570, 1941, 2001, 2625, 2812, 3072
MunI CAATTG 1 cut(s) 1236
Mva1269I GAATGC 2 cut(s) 31, 2688
MvaI CCWGG 7 cut(s) 47, 126, 1941, 2001, 2625, 2812, 3072
MvnI CGCG 2 cut(s) 3189, 3256
MwoI GCNNNNNNNGC 4 cut(s) 11, 1598, 1919, 2519
NciI CCSGG 2 cut(s) 1381, 1570
NcoI CCATGG 4 cut(s) 853, 1100, 1174, 2757
NdeI CATATG 1 cut(s) 635
NheI GCTAGC 1 cut(s) 14
NlaIV GGNNCC 1 cut(s) 535
NmuCI GTSAC 8 cut(s) 35, 492, 796, 1031, 1117, 1809, 2580, 2863
NruI TCGCGA 1 cut(s) 3189
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 5 cut(s) 500, 788, 1251, 1481, 1736
PaeI GCATGC 1 cut(s) 788
PaqCI CACCTGC 1 cut(s) 3289
PasI CCCWGGG 1 cut(s) 2811
PciI ACATGT 2 cut(s) 496, 1732
PciSI GCTCTTC 1 cut(s) 3102
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 2 cut(s) 31, 2688
PdmI GAANNNNTTC 1 cut(s) 211
PflMI CCANNNNNTGG 2 cut(s) 859, 2669
PfoI TCCNGGA 1 cut(s) 1939
PleI GAGTC 7 cut(s) 708, 745, 1872, 2686, 2762, 2794, 2960
PpsI GAGTC 7 cut(s) 708, 745, 1872, 2686, 2762, 2794, 2960
Ppu21I YACGTR 1 cut(s) 978
PpuMI RGGWCCY 1 cut(s) 1058
PscI ACATGT 2 cut(s) 496, 1732
PshAI GACNNNNGTC 1 cut(s) 1709
PsiI TTATAA 1 cut(s) 2576
Psp124BI GAGCTC 1 cut(s) 3274
Psp5II RGGWCCY 1 cut(s) 1058
Psp6I CCWGG 7 cut(s) 45, 124, 1939, 1999, 2623, 2810, 3070
PspEI GGTNACC 1 cut(s) 2863
PspFI CCCAGC 3 cut(s) 575, 962, 1001
PspGI CCWGG 7 cut(s) 45, 124, 1939, 1999, 2623, 2810, 3070
PspN4I GGNNCC 1 cut(s) 535
PspPI GGNCC 3 cut(s) 1058, 1097, 2972
PspPPI RGGWCCY 1 cut(s) 1058
PstI CTGCAG 1 cut(s) 2539
PstNI CAGNNNCTG 4 cut(s) 1035, 2234, 2534, 3242
PsuI RGATCY 4 cut(s) 7, 533, 3002, 3115
PvuII CAGCTG 1 cut(s) 2231
RruI TCGCGA 1 cut(s) 3189
RsaI GTAC 9 cut(s) 338, 397, 759, 991, 1498, 2097, 2998, 3200, 3209
RsaNI GTAC 9 cut(s) 337, 396, 758, 990, 1497, 2096, 2997, 3199, 3208
RseI CAYNNNNRTG 2 cut(s) 945, 1680
SacI GAGCTC 1 cut(s) 3274
SapI GCTCTTC 1 cut(s) 3102
SaqAI TTAA 6 cut(s) 666, 1386, 1451, 1619, 2210, 2747
Sau96I GGNCC 3 cut(s) 1058, 1097, 2972
ScaI AGTACT 1 cut(s) 1498
SchI GAGTC 7 cut(s) 709, 746, 1872, 2687, 2762, 2794, 2960
ScrFI CCNGG 9 cut(s) 47, 126, 1381, 1570, 1941, 2001, 2625, 2812, 3072
SduI GDGCHC 5 cut(s) 574, 1446, 1820, 1887, 3274
SexAI ACCWGGT 1 cut(s) 1999
SfaNI GCATC 4 cut(s) 512, 535, 604, 3293
SfcI CTRYAG 3 cut(s) 1272, 2535, 2775
SinI GGWCC 2 cut(s) 1058, 1097
SmiMI CAYNNNNRTG 2 cut(s) 945, 1680
SmlI CTYRAG 6 cut(s) 112, 1064, 2187, 2311, 2514, 2597
SmoI CTYRAG 6 cut(s) 112, 1064, 2187, 2311, 2514, 2597
SpeI ACTAGT 1 cut(s) 908
SphI GCATGC 1 cut(s) 788
SsiI CCGC 6 cut(s) 301, 464, 923, 1440, 2561, 2913
SstI GAGCTC 1 cut(s) 3274
StyD4I CCNGG 9 cut(s) 45, 124, 1379, 1568, 1939, 1999, 2623, 2810, 3070
StyI CCWWGG 7 cut(s) 725, 792, 853, 1100, 1113, 1174, 2757
TaiI ACGT 6 cut(s) 980, 1397, 2109, 2443, 2844, 3216
TaqI TCGA 8 cut(s) 74, 243, 553, 1693, 1713, 1954, 2291, 2766
TatI WGTACW 3 cut(s) 757, 1496, 3207
TauI GCSGC 3 cut(s) 303, 926, 2564
Tru1I TTAA 6 cut(s) 666, 1386, 1451, 1619, 2210, 2747
Tru9I TTAA 6 cut(s) 666, 1386, 1451, 1619, 2210, 2747
TscAI CASTG 7 cut(s) 1027, 1040, 1270, 1820, 1892, 1932, 2535
TseFI GTSAC 8 cut(s) 35, 492, 796, 1031, 1117, 1809, 2580, 2863
Tsp45I GTSAC 8 cut(s) 35, 492, 796, 1031, 1117, 1809, 2580, 2863
TspDTI ATGAA 6 cut(s) 929, 1185, 1302, 1647, 1884, 2847
TspGWI ACGGA 3 cut(s) 219, 2799, 3206
TspRI CASTG 7 cut(s) 1027, 1040, 1270, 1820, 1892, 1932, 2535
Van91I CCANNNNNTGG 2 cut(s) 859, 2669
VneI GTGCAC 1 cut(s) 1883
VpaK11BI GGWCC 2 cut(s) 1058, 1097
XapI RAATTY 4 cut(s) 935, 1050, 2497, 3010
XceI RCATGY 5 cut(s) 500, 788, 1251, 1481, 1736
XcmI CCANNNNNNNNNTGG 1 cut(s) 748
XmaJI CCTAGG 1 cut(s) 725
XmiI GTMKAC 2 cut(s) 630, 1828
XmnI GAANNNNTTC 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 1498
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.