Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009215

Physical Location & Seq

Genomic Coordinates Forward (+)

2624749 .. 2632164

Gene Structure 7416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1021300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3369 bp

ATGGCAGATCTTGTTAGCTACGGGAATGCCAACCGTGACATCGACCAGGCAATAATTGCTTTAAAGAAGGGTGCTCAACTACTGAAATATGGTCGTAAGGGAAAGCCTAAGTTTTGTCCATTTAGACTTTCTACTGACGAATCATCTTTGATCTGGATTTCAAGTAGTGAAAGAAGTTTGAAGTTGGCTTCTGTCACAAGAATTGTTCCTGGACAAAGAACTGCTGTCTTTCAACGATATTTGTGTCCTGAAAAGGATTATTTATCCTTTTCTCTTATATACAATAACGGAAAGCGGTCTCTTGATCTGATTTGCAAGGACAAAGTTGAGGCAGAAGTGTGGATTGTAGGCCTCAAAGCACTAATTTCCTCTGGTAGTGGTGGTCGTTCCAAAATTGACGGATGGAGTGATGGAGGCCTCTACCTTGATGATGGCAGGGACTTGACATCAAATAGTCAAAGTGATAGTTCTGCTAGTGGTGCACGAGATAGTGGCTCTCCAGAGATTTCTGTTAGTTTCAAAACAAATTCGCCCAAGAGTTTTCTGCCTGAAAATTCCCCGGTTTCTGAGAAGTCACATGCTGCATCAGACCAGACAAAGATGCAAGTAAAAGGATCTGGTTCGGATGCATTTCGAGTTAGTGTTTCAAGTGCTCCTAGCACTTCAAGTCATGGTTCAGCACCGGATGATTGTGAAGCTCTAGGTGATGTGTACATATGGGGTGAAGCTATTTGTGATAGTGTTGTTAAGATTGGGGCTGATAAAAATGTGAATTATTTGAGCCCGAGATCAGATGTGCTTGTCCCTAGGCCATTAGAGTCCAATCTTGTTTTGGATGTCCATCATATAGCCTGTGGGGTCAAACATGCAGCCTTGGTCACGAGACAAGGTGAAGTGTTTACATGGGGAGAAGAATCTGGAGGACGACTAGGACATGGTGCTGGGAAGGATGTTTCTCAGCCTCGTCTAGTTGAATCTCTGGCAGCTACTAGTGTTGATTTTGTGGCATGTGGAGAATTTCATACTTGTGCTGTTACAATGGCTGGGGAACTTTATACATGGGGAGATGGTACGCATAATGCTGGACTTCTTGGTCATGGCACTGATGTCAGCCATTGGATACCAAAGAGAATTTCAGGTCCTCTTGAGGAACTTCAAGTTGCTTCTGTAACTTGTGGTCCATGGCATACAGCCTTGGTAACATCAACCGGGAAACTCTTCACATTTGGCGATGGCACATTTGGTGTCTTGGGCCATGGAGACAGAAAAAACATTCCCTATCCGAGAGAAGTAGAATCCCTATCAGGGTTGAGGACTATATCTGTTGCATGTGGGGTGTGGCACACTGCTGCTGTGGTGGAGGTTATTGCAACACAATCTAGTGCAAGTAGTTCATCAGGTAAGTTGTTTACTTGGGGTGATGGAGATAAGAATCGACTTGGGCATGGAGACAAGGAAGCCCGGCTTAAACCGACATGTGTTCCTGCCCTTATTGACTACAATTTTCAGAAAATTGCTTGTGGGCACAGTTTAACTGTAGGCTTGACGACATCAGGACATGTTTTTACAATGGGCAGTACTGTTTATGGTCAGCTTGGAAATCCCAATTCTGATGGAAAGCTTCCTTGCTTGGTAGAAGACAAGCTTTCTGGGGATTGCGTTGAAGAAATTGCTTGTGGTGCATATCATGTAGCAGTATTAACATCCAGGAATGAAGTTTATACATGGGGAAAGGGAGCTAATGGGAGGTTGGGCCATGGGGATATTGAAGATCGGAAAACACCGACTCTCGTTGAAGCTTTAAAGGATAGACATGTGAAATATATCGGCTGTGGTCAAAACTATACAGCAGCTATTTGTCTTCACAAATGGGTATCTGGTGCTGAGCAGTCACAGTGCTCTGCTTGTAGACAGGCTTTTGGGTTCACAAAGAAGAGGCACAACTGCTATAATTGTGGACTTGTCCACTGCCATTCATGCAGTTCAAGAAAAGCAACAAGAGCAGCACTGGCTCCTAATCCTGGAAAACCATATCGGGTTTGTGATGCTTGTTATGTGAAATTGAATAAGGTGTTAGAAACCGGTGGTAATAACAGAAGGAATGCTATACCTCGCTTGTCAGGTGAGAACAAGGACAGGTTGGACAAGGCTGACATAAGATTGTATAAGTCTGCTGTACTATCTAATATGGATTTGATAAAGCAATTAGATACTAAAGCAGCCAAGCAGGGAAAGAAAGCTGACACATTTTCACTTGTCCGCTCCGCTCAAGCACCTTCATTGTTGCAATTAAAGGATGTTGTTATGTCAACTGCTGTTGATTTGCGACGAACAGTTCCCAAACCAGTTCTTACACCATCTGGAGCGAGTTCAAGGTCCGTGTCACCTTTCTCAAGGAGACCAAGCCCTCCTCGTTTTGCAACTCCTGTTCCTACTACATCAGGACTTTCCTTCTCAAAAAGTATTGCTGATAGTCTGAAGAAAACAAATGAACTTTTGAATCAGGAAGTGCTAAAATTACGTTCACAGGTTGAGAGCCTTAGACAGAGATGTGATATTCAAGAATTAGAGCTTCAGAATTCTTCGAAGAAAGTACAAGAAGCAATGGCATTGGCCGCAGAGGAATCTGCTAAATCTAAAGCTGCAAAAGAAGTTATAAAGTCACTAACCGCACAGCTCAAGGATTTGGCAGAACGGCTGCCACCTGGGGTTTATGATACAGAGACCATAAAGAAGGCATTCCTGCCAAATGGGTTGGAGCCAAATGGCATTAACTATCCAGATGCTAATGAAGAGCAGCATTCAAGATCGAATTCCATCAGCAGTTCTTACTTGATCTCATCCTTGGGAATCGACTCTGCTACAACAAATGGAAATCACGTCCCGATTCATTCACCCAAGGATCCACTTGGAACAAATGAAACAAATGTGCAGCAGAGTCGGGAGCTTCTGACCTCCAATAAACTACCCAATAGTGGTGGATCATTTCAGTCAGTCAGTAGTAGTGTGTCTGAAACTGTTGATGGCAAGGAATCCGGGCCTTTTCAAGATGGGGAGAATGGTGCGAGGTCCAAGAATTCTCCATCGCCTGCTAATGGTAATACAGTTGAGGCAGAATGGATTGAACAATATGAGCCGGGTGTTTATATAACTCTTGTGGCTCTGCGGGATGGAACTAGAGATCTCAAACGAGTGCGCTTCAGCCGAAGGAGATTTGGAGAGCAACAAGCAGAGATCTGGTGGTCGGAGAATCGTGAGAAAGTGTACGAGAAGTACAATGTCCGTGGGTCAGACAAATCATCGGTTGCTGGCTCAGCTGCACGCAGATCAGACGGAGCCCTCTCACCTGCCTCATCTCAACAAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005085 GO:0005086 GO:0005088 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005829 GO:0008150 GO:0008289 GO:0012505 GO:0016020 GO:0017016 GO:0017112 GO:0017137 GO:0019899 GO:0031267 GO:0035091 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051020 GO:0065007 GO:0065009 GO:0070300 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRX BRX_N FYVE PH_12 RCC1

Protein Analysis

1123

Amino Acids

120.93

Weight (kDa)

8.72

Isoelectric Point (pI)

41.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_12	PF16457	16 - 122	4.4e-08	Pleckstrin homology domain
WD40_RLD	PF25390	237 - 618	3.4e-79	RCC1-like domain
RCC1	PF00415	296 - 345	2.5e-11	Regulator of chromosome condensation (RCC1) repeat
Alsin_RLD	PF25384	297 - 408	2.4e-06	Alsin RCC1-like domain
RCC1_2	PF13540	334 - 359	4.8e-06	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	349 - 400	1e-09	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	387 - 415	9.5e-06	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	404 - 452	5.1e-14	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	466 - 514	1.4e-12	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	518 - 567	2.7e-08	Regulator of chromosome condensation (RCC1) repeat
RCC1_2	PF13540	555 - 582	5.5e-07	Regulator of chromosome condensation (RCC1) repeat
RCC1	PF00415	572 - 619	2.8e-14	Regulator of chromosome condensation (RCC1) repeat
FYVE	PF01363	623 - 689	6.9e-15	FYVE zinc finger
BRX_N	PF13713	874 - 909	2.4e-18	Transcription factor BRX N-terminal domain
BRX	PF08381	1037 - 1092	9.5e-29	Transcription factor regulating root and shoot growth via Pin3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0009215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76950 AT5G42140 AT5G42140
fragaria_vesca	FvH4_2g14280 FvH4_2g14280 FvH4_2g14280 FvH4_2g14280
malus_domestica	MD05G1020200.v1.1 MD10G1021300.v1.1
prunus_persica	Prupe.8G024300_v2.0.a1
pyrus_communis	pycom05g01180 pycom10g01440
rosa_chinensis	RchiOBHm_Chr6g0276571
rosa_laevigata	RLG00000013389
rosa_multiflora	Rmu_ssc0000059.1_g000010
rosa_roxburghii	Rroxscaffold_7G00192170
rosa_rugosa	Rorug06G0101800
rosa_samantha	Rh6AG212600 Rh6BG217500 Rh6CG219800 Rh6DG209400
rosa_wichuraiana	Rw6G018580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2657
AarI	CACCTGC	1 cut(s)	3355
AasI	GACNNNNNNGTC	1 cut(s)	1092
Acc36I	ACCTGC	1 cut(s)	3355
AccBSI	CCGCTC	2 cut(s)	2262, 2267
AccI	GTMKAC	1 cut(s)	1909
AciI	CCGC	6 cut(s)	295, 2260, 2265, 2616, 2670, 3166
AclWI	GGATC	4 cut(s)	622, 2897, 2910, 2989
AcoI	YGGCCR	1 cut(s)	2613
AcsI	RAATTY	7 cut(s)	526, 553, 1016, 1131, 2578, 2812, 3076
AcuI	CTGAAG	3 cut(s)	2498, 2558, 3184
AfaI	GTAC	7 cut(s)	713, 1072, 1579, 2178, 2595, 3266, 3275
AfiI	CCNNNNNNNGG	4 cut(s)	1305, 2021, 2975, 3095
AflIII	ACRYGT	3 cut(s)	1475, 1558, 1813
AgeI	ACCGGT	1 cut(s)	2081
AhlI	ACTAGT	1 cut(s)	989
AjiI	CACGTC	1 cut(s)	2881
AjnI	CCWGG	5 cut(s)	45, 208, 1706, 2020, 2704
AjuI	GAANNNNNNNTTGG	2 cut(s)	1117, 1149
Alw21I	GWGCWC	4 cut(s)	76, 484, 655, 1901
Alw26I	GTCTC	6 cut(s)	303, 877, 1254, 1443, 2392, 2717
Alw44I	GTGCAC	1 cut(s)	480
AlwI	GGATC	4 cut(s)	622, 2897, 2910, 2989
Ama87I	CYCGRG	1 cut(s)	784
AoxI	GGCC	7 cut(s)	349, 415, 809, 1252, 1753, 2613, 3038
ApaLI	GTGCAC	1 cut(s)	480
ApoI	RAATTY	7 cut(s)	526, 553, 1016, 1131, 2578, 2812, 3076
AsiGI	ACCGGT	1 cut(s)	2081
Asp700I	GAANNNNTTC	2 cut(s)	1217, 2738
AspA2I	CCTAGG	1 cut(s)	806
AspLEI	GCGC	1 cut(s)	3198
AspS9I	GGNCC	7 cut(s)	1139, 1178, 1252, 1753, 2376, 3038, 3069
AsuC2I	CCSGG	5 cut(s)	560, 1210, 1462, 3037, 3138
AsuHPI	GGTGA	8 cut(s)	716, 734, 902, 1430, 2135, 2376, 2886, 3336
AsuII	TTCGAA	1 cut(s)	2585
AvaI	CYCGRG	1 cut(s)	784
AvaII	GGWCC	4 cut(s)	1139, 1178, 2376, 3069
AvrII	CCTAGG	1 cut(s)	806
BaeGI	GKGCMC	2 cut(s)	484, 1527
BamHI	GGATCC	1 cut(s)	2902
BanII	GRGCYC	2 cut(s)	785, 3340
BauI	CACGAG	2 cut(s)	483, 880
BbsI	GAAGAC	2 cut(s)	1644, 1853
Bbv12I	GWGCWC	4 cut(s)	76, 484, 655, 1901
BceAI	ACGGC	1 cut(s)	2711
BcgI	CGANNNNNNTGC	2 cut(s)	2921, 2955
BciT130I	CCWGG	5 cut(s)	47, 210, 1708, 2022, 2706
BciVI	GTATCC	1 cut(s)	1113
BcnI	CCSGG	5 cut(s)	560, 1210, 1462, 3037, 3138
BcoDI	GTCTC	6 cut(s)	303, 877, 1254, 1443, 2392, 2717
BcuI	ACTAGT	1 cut(s)	989
BfaI	CTAG	9 cut(s)	474, 657, 701, 807, 929, 968, 990, 1380, 3177
BfmI	CTRYAG	1 cut(s)	1536
BfuAI	ACCTGC	1 cut(s)	3355
BfuI	GTATCC	1 cut(s)	1113
BglII	AGATCT	3 cut(s)	7, 3181, 3234
BlnI	CCTAGG	1 cut(s)	806
BlpI	GCTNAGC	2 cut(s)	1884, 3313
BmcAI	AGTACT	1 cut(s)	1579
Bme18I	GGWCC	4 cut(s)	1139, 1178, 2376, 3069
BmeT110I	CYCGRG	1 cut(s)	784
BmgBI	CACGTC	1 cut(s)	2881
BmgT120I	GGNCC	7 cut(s)	1139, 1178, 1252, 1753, 2376, 3038, 3069
BmiI	GGNNCC	4 cut(s)	2013, 2760, 2904, 3337
BmsI	GCATC	5 cut(s)	591, 593, 616, 2035, 2773
BpiI	GAAGAC	2 cut(s)	1644, 1853
BpmI	CTGGAG	3 cut(s)	483, 939, 2382
Bpu1102I	GCTNAGC	2 cut(s)	1884, 3313
Bpu14I	TTCGAA	1 cut(s)	2585
BpuEI	CTTGAG	4 cut(s)	1166, 2253, 2377, 2663
BpuMI	CCSGG	5 cut(s)	560, 1210, 1462, 3037, 3138
BsaBI	GATNNNNATC	2 cut(s)	840, 1431
BsaI	GGTCTC	3 cut(s)	303, 2392, 2717
BsaWI	WCCGGW	2 cut(s)	682, 2081
Bsc4I	CCNNNNNNNGG	4 cut(s)	1305, 2021, 2975, 3095
Bse118I	RCCGGY	1 cut(s)	2081
Bse1I	ACTGG	2 cut(s)	2013, 2345
Bse3DI	GCAATG	1 cut(s)	2610
Bse8I	GATNNNNATC	2 cut(s)	840, 1431
BseBI	CCWGG	5 cut(s)	47, 210, 1708, 2022, 2706
BseGI	GGATG	9 cut(s)	407, 631, 691, 841, 955, 1703, 2302, 2840, 3175
BseJI	GATNNNNATC	2 cut(s)	840, 1431
BseLI	CCNNNNNNNGG	4 cut(s)	1305, 2021, 2975, 3095
BseMI	GCAATG	1 cut(s)	2610
BseMII	CTCAG	4 cut(s)	558, 971, 1875, 3327
BseNI	ACTGG	2 cut(s)	2013, 2345
BseRI	GAGGAG	1 cut(s)	2400
BseSI	GKGCMC	2 cut(s)	484, 1527
BseYI	CCCAGC	2 cut(s)	941, 1043
BsgI	GTGCAG	2 cut(s)	2951, 3303
BshFI	GGCC	7 cut(s)	351, 417, 811, 1254, 1755, 2615, 3040
BshTI	ACCGGT	1 cut(s)	2081
BsiHKAI	GWGCWC	4 cut(s)	76, 484, 655, 1901
BsiHKCI	CYCGRG	1 cut(s)	784
BsiSI	CCGG	7 cut(s)	560, 683, 1209, 1462, 2082, 3036, 3137
BslFI	GGGAC	3 cut(s)	452, 788, 2867
BslI	CCNNNNNNNGG	4 cut(s)	1305, 2021, 2975, 3095
BsmAI	GTCTC	6 cut(s)	303, 877, 1254, 1443, 2392, 2717
BsmFI	GGGAC	3 cut(s)	452, 788, 2867
BsmI	GAATGC	4 cut(s)	31, 2107, 2738, 2800
BsnI	GGCC	7 cut(s)	351, 417, 811, 1254, 1755, 2615, 3040
Bso31I	GGTCTC	3 cut(s)	303, 2392, 2717
BsoBI	CYCGRG	1 cut(s)	784
Bsp119I	TTCGAA	1 cut(s)	2585
Bsp1286I	GDGCHC	7 cut(s)	76, 484, 655, 785, 1527, 1901, 3340
Bsp1407I	TGTACA	1 cut(s)	711
Bsp1720I	GCTNAGC	2 cut(s)	1884, 3313
Bsp19I	CCATGG	3 cut(s)	1181, 1255, 1756
BspACI	CCGC	6 cut(s)	295, 2260, 2265, 2616, 2670, 3166
BspANI	GGCC	7 cut(s)	351, 417, 811, 1254, 1755, 2615, 3040
BspCNI	CTCAG	4 cut(s)	559, 970, 1876, 3326
BspLI	GGNNCC	4 cut(s)	2013, 2760, 2904, 3337
BspMI	ACCTGC	1 cut(s)	3355
BspPI	GGATC	4 cut(s)	622, 2897, 2910, 2989
BspQI	GCTCTTC	1 cut(s)	2787
BspT104I	TTCGAA	1 cut(s)	2585
BspTNI	GGTCTC	3 cut(s)	303, 2392, 2717
BsrBI	CCGCTC	2 cut(s)	2262, 2267
BsrDI	GCAATG	1 cut(s)	2610
BsrFI	RCCGGY	1 cut(s)	2081
BsrGI	TGTACA	1 cut(s)	711
BsrI	ACTGG	2 cut(s)	2013, 2345
BssAI	RCCGGY	1 cut(s)	2081
BssSI	CACGAG	2 cut(s)	483, 880
BssT1I	CCWWGG	8 cut(s)	806, 873, 1181, 1194, 1255, 1756, 2844, 2898
Bst2BI	CACGAG	2 cut(s)	483, 880
Bst2UI	CCWGG	5 cut(s)	47, 210, 1708, 2022, 2706
Bst4CI	ACNGT	8 cut(s)	35, 1529, 1537, 1582, 1896, 2335, 3019, 3106
Bst6I	CTCTTC	3 cut(s)	1223, 1928, 2787
BstAPI	GCANNNNNTGC	1 cut(s)	56
BstAUI	TGTACA	1 cut(s)	711
BstBI	TTCGAA	1 cut(s)	2585
BstC8I	GCNNGC	3 cut(s)	3090, 3310, 3322
BstDEI	CTNAG	7 cut(s)	108, 567, 957, 1884, 2540, 3313, 3366
BstDSI	CCRYGG	4 cut(s)	1181, 1255, 1756, 3283
BstF5I	GGATG	9 cut(s)	407, 631, 691, 841, 955, 1703, 2302, 2840, 3175
BstHHI	GCGC	1 cut(s)	3198
BstMAI	GTCTC	6 cut(s)	303, 877, 1254, 1443, 2392, 2717
BstMWI	GCNNNNNNNGC	8 cut(s)	56, 479, 1679, 1977, 2000, 2009, 2615, 3314
BstNI	CCWGG	5 cut(s)	47, 210, 1708, 2022, 2706
BstNSI	RCATGY	7 cut(s)	581, 869, 1011, 1332, 1479, 1562, 1817
BstSFI	CTRYAG	1 cut(s)	1536
BstSLI	GKGCMC	2 cut(s)	484, 1527
BstV2I	GAAGAC	2 cut(s)	1644, 1853
BstX2I	RGATCY	5 cut(s)	7, 614, 2902, 3181, 3234
BstYI	RGATCY	5 cut(s)	7, 614, 2902, 3181, 3234
BsuI	GTATCC	1 cut(s)	1113
BsuRI	GGCC	7 cut(s)	351, 417, 811, 1254, 1755, 2615, 3040
BtgI	CCRYGG	4 cut(s)	1181, 1255, 1756, 3283
BtgZI	GCGATG	2 cut(s)	1245, 3069
BtrI	CACGTC	1 cut(s)	2881
BtsCI	GGATG	9 cut(s)	407, 631, 691, 841, 955, 1703, 2302, 2840, 3175
BtsI	GCAGTG	2 cut(s)	1344, 1966
BtsIMutI	CAGTG	5 cut(s)	1101, 1344, 1901, 1966, 2006
BveI	ACCTGC	1 cut(s)	3355
Cac8I	GCNNGC	3 cut(s)	3090, 3310, 3322
CfoI	GCGC	1 cut(s)	3198
Cfr10I	RCCGGY	1 cut(s)	2081
Cfr13I	GGNCC	7 cut(s)	1139, 1178, 1252, 1753, 2376, 3038, 3069
Csp6I	GTAC	7 cut(s)	712, 1071, 1578, 2177, 2594, 3265, 3274
CspAI	ACCGGT	1 cut(s)	2081
CspCI	CAANNNNNGTGG	4 cut(s)	2959, 2994, 3265, 3300
CviQI	GTAC	7 cut(s)	712, 1071, 1578, 2177, 2594, 3265, 3274
DdeI	CTNAG	7 cut(s)	108, 567, 957, 1884, 2540, 3313, 3366
DraI	TTTAAA	2 cut(s)	63, 1803
DrdI	GACNNNNNNGTC	1 cut(s)	1092
DseDI	GACNNNNNNGTC	1 cut(s)	1092
EaeI	YGGCCR	1 cut(s)	2613
Eam1104I	CTCTTC	3 cut(s)	1223, 1928, 2787
EarI	CTCTTC	3 cut(s)	1223, 1928, 2787
Eco130I	CCWWGG	8 cut(s)	806, 873, 1181, 1194, 1255, 1756, 2844, 2898
Eco147I	AGGCCT	2 cut(s)	351, 417
Eco24I	GRGCYC	2 cut(s)	785, 3340
Eco31I	GGTCTC	3 cut(s)	303, 2392, 2717
Eco47I	GGWCC	4 cut(s)	1139, 1178, 2376, 3069
Eco57I	CTGAAG	3 cut(s)	2498, 2558, 3184
Eco88I	CYCGRG	1 cut(s)	784
EcoO109I	RGGNCCY	1 cut(s)	1139
EcoRI	GAATTC	3 cut(s)	2578, 2812, 3076
EcoRII	CCWGG	5 cut(s)	45, 208, 1706, 2020, 2704
EcoT14I	CCWWGG	8 cut(s)	806, 873, 1181, 1194, 1255, 1756, 2844, 2898
EcoT22I	ATGCAT	1 cut(s)	631
EcoT38I	GRGCYC	2 cut(s)	785, 3340
ErhI	CCWWGG	8 cut(s)	806, 873, 1181, 1194, 1255, 1756, 2844, 2898
FalI	AAGNNNNNCTT	4 cut(s)	1449, 1481, 1629, 1661
FaqI	GGGAC	3 cut(s)	452, 788, 2867
FauI	CCCGC	1 cut(s)	3159
FauNDI	CATATG	1 cut(s)	716
FblI	GTMKAC	1 cut(s)	1909
FokI	GGATG	9 cut(s)	414, 638, 698, 848, 962, 1690, 2309, 2827, 3182
FriOI	GRGCYC	2 cut(s)	785, 3340
FspBI	CTAG	9 cut(s)	474, 657, 701, 807, 929, 968, 990, 1380, 3177
GlaI	GCGC	1 cut(s)	3197
GsaI	CCCAGC	2 cut(s)	945, 1047
GsuI	CTGGAG	3 cut(s)	483, 939, 2382
HaeIII	GGCC	7 cut(s)	351, 417, 811, 1254, 1755, 2615, 3040
HapII	CCGG	7 cut(s)	560, 683, 1209, 1462, 2082, 3036, 3137
HhaI	GCGC	1 cut(s)	3198
Hin6I	GCGC	1 cut(s)	3196
HinP1I	GCGC	1 cut(s)	3196
HincII	GTYRAC	1 cut(s)	2310
HindII	GTYRAC	1 cut(s)	2310
HindIII	AAGCTT	4 cut(s)	1619, 1643, 1797, 3363
HpaII	CCGG	7 cut(s)	560, 683, 1209, 1462, 2082, 3036, 3137
HphI	GGTGA	8 cut(s)	716, 734, 902, 1430, 2135, 2376, 2886, 3336
Hpy99I	CGWCG	1 cut(s)	2331
HpyAV	CCTTC	7 cut(s)	61, 940, 2091, 2286, 2461, 2728, 3201
HpyCH4III	ACNGT	8 cut(s)	35, 1529, 1537, 1582, 1896, 2335, 3019, 3106
HpyCH4IV	ACGT	2 cut(s)	2521, 2880
HpyF10VI	GCNNNNNNNGC	8 cut(s)	56, 479, 1679, 1977, 2000, 2009, 2615, 3314
HpyF3I	CTNAG	7 cut(s)	108, 567, 957, 1884, 2540, 3313, 3366
HpySE526I	ACGT	2 cut(s)	2521, 2880
HspAI	GCGC	1 cut(s)	3196
LguI	GCTCTTC	1 cut(s)	2787
LmnI	GCTCC	8 cut(s)	658, 1736, 2017, 2267, 2363, 2758, 2944, 3335
LweI	GCATC	5 cut(s)	591, 593, 616, 2035, 2773
MaeI	CTAG	9 cut(s)	474, 657, 701, 807, 929, 968, 990, 1380, 3177
MaeII	ACGT	2 cut(s)	2521, 2880
MbiI	CCGCTC	2 cut(s)	2262, 2267
MflI	RGATCY	5 cut(s)	7, 614, 2902, 3181, 3234
MhlI	GDGCHC	7 cut(s)	76, 484, 655, 785, 1527, 1901, 3340
MlyI	GAGTC	4 cut(s)	827, 1780, 2849, 2947
MmeI	TCCRAC	3 cut(s)	2121, 2736, 3225
Mph1103I	ATGCAT	1 cut(s)	631
MroXI	GAANNNNTTC	2 cut(s)	1217, 2738
MseI	TTAA	8 cut(s)	62, 747, 1467, 1532, 1700, 1802, 2291, 2772
MslI	CAYNNNNRTG	1 cut(s)	1026
MspA1I	CMGCKG	1 cut(s)	3317
MspI	CCGG	7 cut(s)	560, 683, 1209, 1462, 2082, 3036, 3137
Mva1269I	GAATGC	4 cut(s)	31, 2107, 2738, 2800
MvaI	CCWGG	5 cut(s)	47, 210, 1708, 2022, 2706
MwoI	GCNNNNNNNGC	8 cut(s)	56, 479, 1679, 1977, 2000, 2009, 2615, 3314
NciI	CCSGG	5 cut(s)	560, 1210, 1462, 3037, 3138
NcoI	CCATGG	3 cut(s)	1181, 1255, 1756
NdeI	CATATG	1 cut(s)	716
NlaIV	GGNNCC	4 cut(s)	2013, 2760, 2904, 3337
NmuCI	GTSAC	7 cut(s)	35, 193, 573, 877, 1890, 2382, 2661
NsiI	ATGCAT	1 cut(s)	631
NspI	RCATGY	7 cut(s)	581, 869, 1011, 1332, 1479, 1562, 1817
NspV	TTCGAA	1 cut(s)	2585
PaqCI	CACCTGC	1 cut(s)	3355
PceI	AGGCCT	2 cut(s)	351, 417
PciI	ACATGT	3 cut(s)	1475, 1558, 1813
PciSI	GCTCTTC	1 cut(s)	2787
PctI	GAATGC	4 cut(s)	31, 2107, 2738, 2800
PdmI	GAANNNNTTC	2 cut(s)	1217, 2738
PfoI	TCCNGGA	3 cut(s)	208, 1706, 2020
PinAI	ACCGGT	1 cut(s)	2081
PleI	GAGTC	4 cut(s)	826, 1780, 2849, 2946
PpsI	GAGTC	4 cut(s)	826, 1780, 2849, 2946
PpuMI	RGGWCCY	1 cut(s)	1139
PscI	ACATGT	3 cut(s)	1475, 1558, 1813
PsiI	TTATAA	1 cut(s)	2657
Psp5II	RGGWCCY	1 cut(s)	1139
Psp6I	CCWGG	5 cut(s)	45, 208, 1706, 2020, 2704
PspFI	CCCAGC	2 cut(s)	941, 1043
PspGI	CCWGG	5 cut(s)	45, 208, 1706, 2020, 2704
PspN4I	GGNNCC	4 cut(s)	2013, 2760, 2904, 3337
PspPI	GGNCC	7 cut(s)	1139, 1178, 1252, 1753, 2376, 3038, 3069
PspPPI	RGGWCCY	1 cut(s)	1139
PsuI	RGATCY	5 cut(s)	7, 614, 2902, 3181, 3234
PvuII	CAGCTG	1 cut(s)	3317
RsaI	GTAC	7 cut(s)	713, 1072, 1579, 2178, 2595, 3266, 3275
RsaNI	GTAC	7 cut(s)	712, 1071, 1578, 2177, 2594, 3265, 3274
RseI	CAYNNNNRTG	1 cut(s)	1026
SapI	GCTCTTC	1 cut(s)	2787
SaqAI	TTAA	8 cut(s)	62, 747, 1467, 1532, 1700, 1802, 2291, 2772
Sau96I	GGNCC	7 cut(s)	1139, 1178, 1252, 1753, 2376, 3038, 3069
ScaI	AGTACT	1 cut(s)	1579
SchI	GAGTC	4 cut(s)	827, 1780, 2849, 2947
SduI	GDGCHC	7 cut(s)	76, 484, 655, 785, 1527, 1901, 3340
SfaNI	GCATC	5 cut(s)	591, 593, 616, 2035, 2773
SfcI	CTRYAG	1 cut(s)	1536
SfuI	TTCGAA	1 cut(s)	2585
SinI	GGWCC	4 cut(s)	1139, 1178, 2376, 3069
SmiMI	CAYNNNNRTG	1 cut(s)	1026
SmlI	CTYRAG	4 cut(s)	1145, 2268, 2392, 2678
SmoI	CTYRAG	4 cut(s)	1145, 2268, 2392, 2678
SpeI	ACTAGT	1 cut(s)	989
SseBI	AGGCCT	2 cut(s)	351, 417
SsiI	CCGC	6 cut(s)	295, 2260, 2265, 2616, 2670, 3166
SspMI	CTAG	9 cut(s)	474, 657, 701, 807, 929, 968, 990, 1380, 3177
StuI	AGGCCT	2 cut(s)	351, 417
StyI	CCWWGG	8 cut(s)	806, 873, 1181, 1194, 1255, 1756, 2844, 2898
TaaI	ACNGT	8 cut(s)	35, 1529, 1537, 1582, 1896, 2335, 3019, 3106
TaiI	ACGT	2 cut(s)	2524, 2883
TaqI	TCGA	6 cut(s)	42, 634, 1435, 2585, 2810, 2853
TatI	WGTACW	5 cut(s)	711, 1577, 2176, 2593, 3273
TauI	GCSGC	1 cut(s)	2618
Tru1I	TTAA	8 cut(s)	62, 747, 1467, 1532, 1700, 1802, 2291, 2772
Tru9I	TTAA	8 cut(s)	62, 747, 1467, 1532, 1700, 1802, 2291, 2772
TscAI	CASTG	5 cut(s)	1108, 1351, 1901, 1973, 2013
TseFI	GTSAC	7 cut(s)	35, 193, 573, 877, 1890, 2382, 2661
Tsp45I	GTSAC	7 cut(s)	35, 193, 573, 877, 1890, 2382, 2661
TspDTI	ATGAA	9 cut(s)	1010, 1383, 1728, 1965, 2268, 2505, 2805, 2879, 2934
TspGWI	ACGGA	5 cut(s)	303, 414, 2368, 3272, 3348
TspRI	CASTG	5 cut(s)	1108, 1351, 1901, 1973, 2013
VneI	GTGCAC	1 cut(s)	480
VpaK11BI	GGWCC	4 cut(s)	1139, 1178, 2376, 3069
XapI	RAATTY	7 cut(s)	526, 553, 1016, 1131, 2578, 2812, 3076
XceI	RCATGY	7 cut(s)	581, 869, 1011, 1332, 1479, 1562, 1817
XcmI	CCANNNNNNNNNTGG	1 cut(s)	829
XmaJI	CCTAGG	1 cut(s)	806
XmiI	GTMKAC	1 cut(s)	1909
XmnI	GAANNNNTTC	2 cut(s)	1217, 2738
XspI	CTAG	9 cut(s)	474, 657, 701, 807, 929, 968, 990, 1380, 3177
ZrmI	AGTACT	1 cut(s)	1579
Zsp2I	ATGCAT	1 cut(s)	631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.