AT1G77290

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010338
Physical Location & Seq
Forward (+)
29038366 .. 29040217
1852 bp
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UTR
Exon/CDS
Intron
AT1G77290.1

Sequence Viewer

Length: 801 bp
ATGCAGTTATATCACCATCCTTACTCAATAGACAGCCAAAGAGTGAGACTAGCTTTGGAAGAGAAAGGCATTGATTACACATCATACCATGTCAATCCCATCACAGGCAAGCACATGGACCCTTCCTTCTTTAGGATGAATCCCAACGCCAAACTTCCCGTTTTCAGGAATGGTTCTCACATCATTTTGGACACTATTGAGATCATAGAGTACTTAGAGAGAATAGCTGAAGTTTCCTCTGGTATAGAAGATGCTACTTTTAATAGAGAAGTTGTTGAATGGATGCGGAAGATTAGGGAATGGGAGTCCAAACTTTTCACTCTTGCTCACATACCGGATAATCGTCGCCTCTATGTTTCCAAGTTCTTGAGGATGGTAGTGATTGCAAGGATGGCTGAGTCTCCAGACTTAGCTAGTGCTTACCATAGGAAGCTAAGGGAAGCATACGATACAGAAGACAAACTAAAAGACCCTGGGGCCTTAAGGAGAAGCAAAGACCATTTGCTTAGGCTTCTAGATGAGGTTGAAACAAAGCTAGAGGGGACAACCTATTTGGCTGGGAATGAGTTTAGTATGGCTGATGTAATGCTAATCCCGGTGCTAGCTCGGTTATCGCTCTTGGATTTGGAAGAGGAGTATATAAGTAGCAGGAAGAATCTTGCTGAGTATTGGGCTCTTGTGAGGAGGCGGCCAAGTTACAAGAAGGTAATTGGAAGGTACTTCAATGGCTGGAGAAAATATGCAACACTTGTAAAAACTTGGATGTTTGTTAGAGTCAGAAGTTTACTCAGAAAATATTGA

Protein Analysis

266

Amino Acids

31.51

Weight (kDa)

9.44

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 74 3.7e-12 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 3 - 77 9.4e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 74 1.8e-13 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 160 - 223 1.2e-10 Glutathione S-transferase, C-terminal domain
GST_C PF00043 164 - 231 2.7e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 286, 688
AcoI YGGCCR 1 cut(s) 689
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 212, 719
AfiI CCNNNNNNNGG 3 cut(s) 104, 132, 165
AflII CTTAAG 1 cut(s) 481
AgsI TTSAA 3 cut(s) 278, 527, 724
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 6 cut(s) 53, 227, 413, 433, 535, 605
AluI AGCT 6 cut(s) 53, 227, 413, 433, 535, 605
Alw26I GTCTC 2 cut(s) 40, 405
AoxI GGCC 2 cut(s) 477, 689
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
AspS9I GGNCC 2 cut(s) 118, 477
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 1 cut(s) 5
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 462
BccI CCATC 4 cut(s) 24, 107, 367, 385
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 596
BcoDI GTCTC 2 cut(s) 40, 405
BfaI CTAG 5 cut(s) 50, 414, 515, 536, 602
BfrI CTTAAG 1 cut(s) 481
BisI GCNGC 1 cut(s) 689
BlsI GCNGC 1 cut(s) 690
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 2 cut(s) 474, 596
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 118, 477
BmiI GGNNCC 2 cut(s) 120, 478
BmrFI CCNGG 2 cut(s) 474, 596
BmsI GCATC 2 cut(s) 241, 273
BmtI GCTAGC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 2 cut(s) 387, 751
Bpu10I CCTNAGC 2 cut(s) 434, 506
BpuEI CTTGAG 1 cut(s) 388
BpuMI CCSGG 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 472, 473
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 132, 165
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 472, 473
BseGI GGATG 6 cut(s) 16, 141, 288, 378, 396, 768
BseLI CCNNNNNNNGG 3 cut(s) 104, 132, 165
BseMII CTCAG 2 cut(s) 387, 654
BseRI GAGGAG 2 cut(s) 647, 697
BseYI CCCAGC 1 cut(s) 557
BshFI GGCC 2 cut(s) 479, 691
BsiSI CCGG 2 cut(s) 335, 596
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 3 cut(s) 104, 132, 165
BsmAI GTCTC 2 cut(s) 40, 405
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 2 cut(s) 479, 691
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 1 cut(s) 201
BspACI CCGC 2 cut(s) 286, 688
BspANI GGCC 2 cut(s) 479, 691
BspCNI CTCAG 3 cut(s) 388, 655, 801
BspLI GGNNCC 2 cut(s) 120, 478
BspOI GCTAGC 1 cut(s) 605
BspTI CTTAAG 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 472, 473
BssMI GATC 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 474
Bst6I CTCTTC 2 cut(s) 54, 624
BstAFI CTTAAG 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 110, 603
BstDEI CTNAG 7 cut(s) 214, 396, 409, 434, 506, 663, 788
BstENI CCTNNNNNAGG 1 cut(s) 130
BstF5I GGATG 6 cut(s) 16, 141, 288, 378, 396, 768
BstKTI GATC 1 cut(s) 204
BstMAI GTCTC 2 cut(s) 40, 405
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 2 cut(s) 472, 594
BstV2I GAAGAC 1 cut(s) 462
BsuRI GGCC 2 cut(s) 479, 691
BtsCI GGATG 6 cut(s) 16, 141, 288, 378, 396, 768
Cac8I GCNNGC 2 cut(s) 110, 603
Cfr13I GGNCC 2 cut(s) 118, 477
Csp6I GTAC 2 cut(s) 211, 718
CviAII CATG 2 cut(s) 89, 115
CviQI GTAC 2 cut(s) 211, 718
DdeI CTNAG 7 cut(s) 214, 396, 409, 434, 506, 663, 788
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 689
Eam1104I CTCTTC 2 cut(s) 54, 624
EarI CTCTTC 2 cut(s) 54, 624
Eco24I GRGCYC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 249
EcoNI CCTNNNNNAGG 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 676
FaeI CATG 2 cut(s) 92, 118
FaqI GGGAC 1 cut(s) 556
FatI CATG 2 cut(s) 88, 114
Fnu4HI GCNGC 1 cut(s) 689
FokI GGATG 6 cut(s) 3, 148, 295, 385, 403, 775
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 1 cut(s) 689
FspBI CTAG 5 cut(s) 50, 414, 515, 536, 602
GluI GCNGC 1 cut(s) 689
GsaI CCCAGC 1 cut(s) 561
GsuI CTGGAG 2 cut(s) 387, 751
HaeIII GGCC 2 cut(s) 479, 691
HapII CCGG 2 cut(s) 335, 596
Hin1II CATG 2 cut(s) 92, 118
HinfI GANTC 5 cut(s) 139, 305, 398, 655, 774
HpaII CCGG 2 cut(s) 335, 596
HphI GGTGA 1 cut(s) 5
Hpy166II GTNNAC 1 cut(s) 785
Hpy188I TCNGA 2 cut(s) 779, 791
Hpy188III TCNNGA 4 cut(s) 166, 367, 404, 515
Hpy8I GTNNAC 1 cut(s) 785
Hpy99I CGWCG 1 cut(s) 348
HpyAV CCTTC 4 cut(s) 132, 136, 697, 708
HpyCH4V TGCA 3 cut(s) 4, 386, 743
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 7 cut(s) 214, 396, 409, 434, 506, 663, 788
Hsp92II CATG 2 cut(s) 92, 118
Kzo9I GATC 1 cut(s) 201
LweI GCATC 2 cut(s) 241, 273
MaeI CTAG 5 cut(s) 50, 414, 515, 536, 602
MaeIII GTNAC 1 cut(s) 695
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MboII GAAGA 6 cut(s) 71, 260, 301, 467, 641, 664
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 1 cut(s) 708
MlyI GAGTC 3 cut(s) 314, 407, 783
MnlI CCTC 8 cut(s) 247, 359, 363, 514, 532, 625, 675, 678
MseI TTAA 2 cut(s) 261, 482
MspCI CTTAAG 1 cut(s) 481
MspI CCGG 2 cut(s) 335, 596
MspR9I CCNGG 2 cut(s) 474, 596
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 392
NciI CCSGG 1 cut(s) 596
NdeII GATC 1 cut(s) 201
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 2 cut(s) 92, 118
NlaIV GGNNCC 2 cut(s) 120, 478
PasI CCCWGGG 1 cut(s) 473
PfeI GAWTC 2 cut(s) 139, 655
PkrI GCNGC 1 cut(s) 690
PleI GAGTC 3 cut(s) 313, 406, 782
PpsI GAGTC 3 cut(s) 313, 406, 782
Psp6I CCWGG 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 557
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 2 cut(s) 120, 478
PspPI GGNCC 2 cut(s) 118, 477
RsaI GTAC 2 cut(s) 212, 719
RsaNI GTAC 2 cut(s) 211, 718
SaqAI TTAA 2 cut(s) 261, 482
SatI GCNGC 1 cut(s) 689
Sau3AI GATC 1 cut(s) 201
Sau96I GGNCC 2 cut(s) 118, 477
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 3 cut(s) 314, 407, 783
ScrFI CCNGG 2 cut(s) 474, 596
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 2 cut(s) 241, 273
SinI GGWCC 1 cut(s) 118
SmlI CTYRAG 2 cut(s) 367, 481
SmoI CTYRAG 2 cut(s) 367, 481
Sse9I AATT 1 cut(s) 708
SsiI CCGC 2 cut(s) 286, 688
SspI AATATT 1 cut(s) 797
SspMI CTAG 5 cut(s) 50, 414, 515, 536, 602
StyD4I CCNGG 2 cut(s) 472, 594
TasI AATT 1 cut(s) 708
TatI WGTACW 1 cut(s) 210
TauI GCSGC 1 cut(s) 691
TfiI GAWTC 2 cut(s) 139, 655
Tru1I TTAA 2 cut(s) 261, 482
Tru9I TTAA 2 cut(s) 261, 482
TspDTI ATGAA 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 481
VpaK11BI GGWCC 1 cut(s) 118
XagI CCTNNNNNAGG 1 cut(s) 130
XbaI TCTAGA 1 cut(s) 514
XspI CTAG 5 cut(s) 50, 414, 515, 536, 602
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.