Rorug06G0265600

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010338
Physical Location & Seq
Reverse (-)
41200010 .. 41200934
925 bp
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UTR
Exon/CDS
Intron
Rorug06G0265600.1

Sequence Viewer

Length: 534 bp
ATGGCAGCGGTCTATCTCTTTTCCTCTGCGACTAGTCTTCCCACCCTTTGGAGCTCGCTGTCACCGCCATTGCCTCAAACCGAAGAAGTTCTCGGTACCCTACCCGGTAGAACTCGTTTTCTGAATAAGTGTTCAGTTTCAACAAGTTTTTCTGTACAAACTTCCTTGCCGGAGAATAGCGATAGATTTAAAGTGGAATACACTCCTTGGATGATTGTTGGATTGGGTAACTCTGGAAATAAGTACCATGGAACTAGGCACAATGTTGGTTTTGAAATGATAGATAGTATTTCCAAAACGCAGGGCATTGTGATGAATACAATTCAGTCAAAGGCACTGGTTGGAATAGGTCGGTCTTCAGTCCTTGTAACTTTTATTTTCATACTCTTATACGTGTTGGTGTTCATTCAGATTTCTTGGTGTCATCAGTACAGGTTCATTGGAGAGGTACCAATTTTGGTGGTGAAGCCTCAAGCTTACATGAATTATAGTGGGGAAGCGGTGTGTTCTCATCATCTTTGTTTGCTATCTTGA

Protein Analysis

177

Amino Acids

19.64

Weight (kDa)

8.72

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pept_tRNA_hydro PF01195 71 - 169 1.1e-17 Peptidyl-tRNA hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 95, 448
AccB1I GGYRCC 2 cut(s) 95, 448
AccB7I CCANNNNNTGG 1 cut(s) 48
AciI CCGC 3 cut(s) 8, 65, 500
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 5 cut(s) 97, 156, 245, 431, 450
AfiI CCNNNNNNNGG 1 cut(s) 48
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 2 cut(s) 141, 275
AhlI ACTAGT 1 cut(s) 32
AluBI AGCT 2 cut(s) 54, 476
AluI AGCT 2 cut(s) 54, 476
Alw21I GWGCWC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 87
Asp718I GGTACC 2 cut(s) 95, 448
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 54, 475
BanI GGYRCC 2 cut(s) 95, 448
BanII GRGCYC 1 cut(s) 56
BbsI GAAGAC 2 cut(s) 29, 348
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 17
BcnI CCSGG 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 2 cut(s) 33, 255
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 105
BmiI GGNNCC 2 cut(s) 97, 450
BmrFI CCNGG 1 cut(s) 105
BpiI GAAGAC 2 cut(s) 29, 348
BpuEI CTTGAG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 206, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse1I ACTGG 1 cut(s) 342
Bse3DI GCAATG 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 206, 247
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMI GCAATG 1 cut(s) 68
BseNI ACTGG 1 cut(s) 342
BseXI GCAGC 1 cut(s) 17
BshNI GGYRCC 2 cut(s) 95, 448
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 105, 170
BslI CCNNNNNNNGG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 154
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 3 cut(s) 8, 65, 500
BspLI GGNNCC 2 cut(s) 97, 450
BspT107I GGYRCC 2 cut(s) 95, 448
BsrDI GCAATG 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 1 cut(s) 342
BssECI CCNNGG 2 cut(s) 206, 247
BssT1I CCWWGG 2 cut(s) 206, 247
BstAUI TGTACA 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 56
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstSCI CCNGG 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 2 cut(s) 29, 348
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 335
Cac8I GCNNGC 1 cut(s) 56
Csp6I GTAC 5 cut(s) 96, 155, 244, 430, 449
CspCI CAANNNNNGTGG 2 cut(s) 441, 476
CviAII CATG 2 cut(s) 248, 481
CviJI RGCY 3 cut(s) 54, 469, 476
CviKI_1 RGCY 3 cut(s) 54, 469, 476
CviQI GTAC 5 cut(s) 96, 155, 244, 430, 449
DraI TTTAAA 1 cut(s) 190
Ecl136II GAGCTC 1 cut(s) 54
Eco130I CCWWGG 2 cut(s) 206, 247
Eco24I GRGCYC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 54
EcoT14I CCWWGG 2 cut(s) 206, 247
EcoT38I GRGCYC 1 cut(s) 56
ErhI CCWWGG 2 cut(s) 206, 247
FaeI CATG 2 cut(s) 251, 484
FaiI YATR 5 cut(s) 249, 383, 391, 482, 489
FatI CATG 2 cut(s) 247, 480
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 1 cut(s) 223
FriOI GRGCYC 1 cut(s) 56
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 33, 255
GluI GCNGC 1 cut(s) 6
HapII CCGG 2 cut(s) 105, 170
Hin1II CATG 2 cut(s) 251, 484
HindIII AAGCTT 1 cut(s) 474
HpaII CCGG 2 cut(s) 105, 170
HphI GGTGA 2 cut(s) 54, 475
Hpy188I TCNGA 2 cut(s) 123, 411
Hpy188III TCNNGA 2 cut(s) 234, 531
HpyCH4IV ACGT 1 cut(s) 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 2 cut(s) 251, 484
KpnI GGTACC 2 cut(s) 99, 452
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 6 cut(s) 118, 183, 219, 287, 323, 418
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 2 cut(s) 33, 255
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 3 cut(s) 60, 227, 367
MboII GAAGA 3 cut(s) 29, 95, 348
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 3 cut(s) 321, 453, 484
MmeI TCCRAC 2 cut(s) 199, 322
MnlI CCTC 4 cut(s) 34, 84, 439, 480
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 105, 170
MspR9I CCNGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 105
NcoI CCATGG 1 cut(s) 247
NlaIII CATG 2 cut(s) 251, 484
NlaIV GGNNCC 2 cut(s) 97, 450
NmuCI GTSAC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 87
PflMI CCANNNNNTGG 1 cut(s) 48
PkrI GCNGC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 97, 450
RsaI GTAC 5 cut(s) 97, 156, 245, 431, 450
RsaNI GTAC 5 cut(s) 96, 155, 244, 430, 449
RseI CAYNNNNRTG 1 cut(s) 311
SacI GAGCTC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 56
SetI ASST 6 cut(s) 56, 352, 396, 437, 450, 478
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 3 cut(s) 321, 453, 484
SsiI CCGC 3 cut(s) 8, 65, 500
SspMI CTAG 2 cut(s) 33, 255
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 2 cut(s) 206, 247
TaiI ACGT 1 cut(s) 396
TaqII GACCGA 1 cut(s) 342
TasI AATT 3 cut(s) 321, 453, 484
TatI WGTACW 2 cut(s) 154, 429
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 5 cut(s) 329, 370, 394, 427, 497
TspRI CASTG 1 cut(s) 342
Van91I CCANNNNNTGG 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 2 cut(s) 33, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.