MD15G1133600.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010338
Physical Location & Seq
Forward (+)
9698962 .. 9701045
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1133600.v1.1.491

Sequence Viewer

Length: 804 bp
ATGCAGTTATATCACCATCCTTACTCATTGGATAGCCAAAGAGTGAGACTTGCTTTGGAGGAAAAAGGCATTGATTACACATCATTCCATGTCAATCCCGTGACGGTCAAGAACATGGATGCCTCATTCTTCAGGAGGAATCCGAGTGCAAAACTCCCGGTTTTCCAGAACGGGCACCATATCATTTACAACACCATTGAGATCATTCAGTATGTGGAAAGAATTGCACTAGTCTCATCAGGGGAAGAGAACATCCCTTCAAGTGGCGGAGAAGTGACTGCATGGATGCACAGAATAAAACAATGGAACCCCAAGTTCTTTACGCTTTCTCACATCCCAAAGAAGCACCGCGATTATGTCTATAAGTTCATAAGGCAGGTGATTATTGCTCGAATGGCGGAAGCTACTGATCTAGCTGCTGCTTATCACGCCAAGTTAGTCCAAGTGTACGAGACAGAAGACAAGTTAAAAGACCCCGCGGTTTTGAACCAGAGCAAGGAACAGTTAATAAGACTTCTTGATCAAGTCGAAAAAAAGCTGAACGAATCGGCTTATTTGGCCGGGGAAGAGTTTAGCATGGCGGATGTGATGCTCGTTCCTGTGCTGGCACGGCTAGTGCTCTTGAATTTGAAGGAAGAGTACATAGATGGCCGGCCGAACACGGCTAAATACTGGATTATGGTGCAGCAGAGGCCTAGTTATAAGAAGGTGATTGGGAAGCACTTCAGTGGGTGGAGGAAGCACAAAACATTTATTAGAACTTGGTGTTTTGTTCACATCAGAACCATTCTAAGGAGATTTTGA

Protein Analysis

268

Amino Acids

31.37

Weight (kDa)

9.63

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 74 5.5e-10 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 3 - 75 5e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 74 8.7e-10 Glutathione S-transferase, N-terminal domain
GST_C_3 PF14497 147 - 236 1.9e-07 Glutathione S-transferase, C-terminal domain
GST_C PF00043 157 - 232 8e-10 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 160 - 224 8.2e-09 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AccB1I GGYRCC 1 cut(s) 174
AccII CGCG 2 cut(s) 351, 479
AciI CCGC 6 cut(s) 267, 349, 398, 477, 479, 581
AcoI YGGCCR 3 cut(s) 558, 649, 653
AcsI RAATTY 1 cut(s) 625
AcuI CTGAAG 2 cut(s) 115, 709
AfaI GTAC 2 cut(s) 449, 641
AfiI CCNNNNNNNGG 3 cut(s) 263, 496, 792
AgsI TTSAA 4 cut(s) 261, 487, 625, 631
AhlI ACTAGT 1 cut(s) 229
AleI CACNNNNGTG 1 cut(s) 726
AluBI AGCT 3 cut(s) 404, 416, 538
AluI AGCT 3 cut(s) 404, 416, 538
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 3 cut(s) 40, 238, 446
AoxI GGCC 4 cut(s) 558, 649, 653, 692
ApeKI GCWGC 3 cut(s) 416, 419, 685
ApoI RAATTY 1 cut(s) 625
Asp700I GAANNNNTTC 1 cut(s) 722
AsuC2I CCSGG 2 cut(s) 158, 562
AsuHPI GGTGA 3 cut(s) 5, 391, 721
BaeGI GKGCMC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 174
BarI GAAGNNNNNNTAC 2 cut(s) 623, 655
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 3 cut(s) 403, 406, 697
BccI CCATC 2 cut(s) 24, 641
BceAI ACGGC 2 cut(s) 626, 678
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 2 cut(s) 158, 562
BcoDI GTCTC 3 cut(s) 40, 238, 446
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 4 cut(s) 230, 413, 614, 696
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 3 cut(s) 417, 420, 686
BlsI GCNGC 3 cut(s) 418, 421, 687
Bme1390I CCNGG 2 cut(s) 158, 562
BmiI GGNNCC 2 cut(s) 176, 308
BmrFI CCNGG 2 cut(s) 158, 562
BmsI GCATC 3 cut(s) 109, 276, 579
BpiI GAAGAC 1 cut(s) 465
BpuMI CCSGG 2 cut(s) 158, 562
BsaJI CCNNGG 2 cut(s) 477, 561
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 496, 792
Bse118I RCCGGY 1 cut(s) 651
Bse1I ACTGG 1 cut(s) 677
BseDI CCNNGG 2 cut(s) 477, 561
BseGI GGATG 6 cut(s) 16, 124, 252, 291, 333, 589
BseLI CCNNNNNNNGG 3 cut(s) 263, 496, 792
BseNI ACTGG 1 cut(s) 677
BseSI GKGCMC 1 cut(s) 177
BseX3I CGGCCG 1 cut(s) 653
BseXI GCAGC 3 cut(s) 403, 406, 697
BsgI GTGCAG 1 cut(s) 704
Bsh1236I CGCG 2 cut(s) 351, 479
Bsh1285I CGRYCG 1 cut(s) 656
BshFI GGCC 4 cut(s) 560, 651, 655, 694
BshNI GGYRCC 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 3 cut(s) 158, 561, 652
BslI CCNNNNNNNGG 3 cut(s) 263, 496, 792
BsmAI GTCTC 3 cut(s) 40, 238, 446
BsnI GGCC 4 cut(s) 560, 651, 655, 694
Bsp1286I GDGCHC 2 cut(s) 177, 621
Bsp143I GATC 3 cut(s) 201, 409, 520
BspACI CCGC 6 cut(s) 267, 349, 398, 477, 479, 581
BspANI GGCC 4 cut(s) 560, 651, 655, 694
BspFNI CGCG 2 cut(s) 351, 479
BspLI GGNNCC 2 cut(s) 176, 308
BspMI ACCTGC 1 cut(s) 367
BspT107I GGYRCC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 651
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 651
BssECI CCNNGG 2 cut(s) 477, 561
BssMI GATC 3 cut(s) 201, 409, 520
Bst4CI ACNGT 2 cut(s) 106, 504
Bst6I CTCTTC 3 cut(s) 240, 561, 630
BstC8I GCNNGC 2 cut(s) 606, 653
BstDEI CTNAG 1 cut(s) 791
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 6 cut(s) 16, 124, 252, 291, 333, 589
BstFNI CGCG 2 cut(s) 351, 479
BstKTI GATC 3 cut(s) 204, 412, 523
BstMAI GTCTC 3 cut(s) 40, 238, 446
BstMBI GATC 3 cut(s) 201, 409, 520
BstMCI CGRYCG 1 cut(s) 656
BstMWI GCNNNNNNNGC 5 cut(s) 395, 428, 557, 610, 691
BstSCI CCNGG 2 cut(s) 156, 560
BstSLI GKGCMC 1 cut(s) 177
BstUI CGCG 2 cut(s) 351, 479
BstV1I GCAGC 3 cut(s) 403, 406, 697
BstV2I GAAGAC 1 cut(s) 465
BstZI CGGCCG 1 cut(s) 653
BsuRI GGCC 4 cut(s) 560, 651, 655, 694
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 6 cut(s) 16, 124, 252, 291, 333, 589
BtsIMutI CAGTG 1 cut(s) 733
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 2 cut(s) 606, 653
Cfr10I RCCGGY 1 cut(s) 651
Cfr42I CCGCGG 1 cut(s) 480
Csp6I GTAC 2 cut(s) 448, 640
CviAII CATG 4 cut(s) 89, 115, 282, 577
CviQI GTAC 2 cut(s) 448, 640
DdeI CTNAG 1 cut(s) 791
DpnI GATC 3 cut(s) 203, 411, 522
DpnII GATC 3 cut(s) 201, 409, 520
EaeI YGGCCR 3 cut(s) 558, 649, 653
EagI CGGCCG 1 cut(s) 653
Eam1104I CTCTTC 3 cut(s) 240, 561, 630
EarI CTCTTC 3 cut(s) 240, 561, 630
EciI GGCGGA 3 cut(s) 282, 413, 596
EclXI CGGCCG 1 cut(s) 653
Eco147I AGGCCT 1 cut(s) 694
Eco52I CGGCCG 1 cut(s) 653
Eco57I CTGAAG 2 cut(s) 115, 709
FaeI CATG 4 cut(s) 92, 118, 285, 580
FatI CATG 4 cut(s) 88, 114, 281, 576
FauI CCCGC 1 cut(s) 484
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 3 cut(s) 417, 420, 686
FokI GGATG 6 cut(s) 3, 131, 239, 298, 320, 596
FseI GGCCGGCC 1 cut(s) 655
Fsp4HI GCNGC 3 cut(s) 417, 420, 686
FspBI CTAG 4 cut(s) 230, 413, 614, 696
GluI GCNGC 3 cut(s) 417, 420, 686
HaeIII GGCC 4 cut(s) 560, 651, 655, 694
HapII CCGG 3 cut(s) 158, 561, 652
Hin1II CATG 4 cut(s) 92, 118, 285, 580
HinfI GANTC 2 cut(s) 139, 545
HpaII CCGG 3 cut(s) 158, 561, 652
HphI GGTGA 3 cut(s) 5, 391, 721
Hpy166II GTNNAC 2 cut(s) 448, 775
Hpy188I TCNGA 2 cut(s) 144, 782
Hpy188III TCNNGA 5 cut(s) 109, 133, 166, 518, 622
Hpy8I GTNNAC 2 cut(s) 448, 775
HpyAV CCTTC 3 cut(s) 267, 625, 700
HpyCH4III ACNGT 2 cut(s) 106, 504
HpyCH4V TGCA 6 cut(s) 4, 149, 227, 281, 289, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 395, 428, 557, 610, 691
HpyF3I CTNAG 1 cut(s) 791
Hsp92II CATG 4 cut(s) 92, 118, 285, 580
KroI GCCGGC 1 cut(s) 651
KroNI GCCGGC 1 cut(s) 653
Ksp22I TGATCA 1 cut(s) 520
KspI CCGCGG 1 cut(s) 480
Kzo9I GATC 3 cut(s) 201, 409, 520
Lsp1109I GCAGC 3 cut(s) 403, 406, 697
LweI GCATC 3 cut(s) 109, 276, 579
MaeI CTAG 4 cut(s) 230, 413, 614, 696
MaeIII GTNAC 2 cut(s) 100, 274
MalI GATC 3 cut(s) 203, 411, 522
MboI GATC 3 cut(s) 201, 409, 520
MboII GAAGA 5 cut(s) 121, 257, 470, 578, 647
MhlI GDGCHC 2 cut(s) 177, 621
MluCI AATT 2 cut(s) 222, 625
MnlI CCTC 5 cut(s) 52, 129, 133, 684, 729
MroNI GCCGGC 1 cut(s) 651
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 2 cut(s) 467, 506
MslI CAYNNNNRTG 1 cut(s) 726
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 3 cut(s) 158, 561, 652
MspR9I CCNGG 2 cut(s) 158, 562
MvnI CGCG 2 cut(s) 351, 479
MwoI GCNNNNNNNGC 5 cut(s) 395, 428, 557, 610, 691
NaeI GCCGGC 1 cut(s) 653
NciI CCSGG 2 cut(s) 158, 562
NdeII GATC 3 cut(s) 201, 409, 520
NgoMIV GCCGGC 1 cut(s) 651
NlaIII CATG 4 cut(s) 92, 118, 285, 580
NlaIV GGNNCC 2 cut(s) 176, 308
NmuCI GTSAC 2 cut(s) 100, 274
OliI CACNNNNGTG 1 cut(s) 726
PaqCI CACCTGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 694
PdiI GCCGGC 1 cut(s) 653
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 2 cut(s) 139, 545
PkrI GCNGC 3 cut(s) 418, 421, 687
PsiI TTATAA 1 cut(s) 702
PspN4I GGNNCC 2 cut(s) 176, 308
RigI GGCCGGCC 1 cut(s) 655
RsaI GTAC 2 cut(s) 449, 641
RsaNI GTAC 2 cut(s) 448, 640
RseI CAYNNNNRTG 1 cut(s) 726
SacII CCGCGG 1 cut(s) 480
SaqAI TTAA 2 cut(s) 467, 506
SatI GCNGC 3 cut(s) 417, 420, 686
Sau3AI GATC 3 cut(s) 201, 409, 520
ScrFI CCNGG 2 cut(s) 158, 562
SduI GDGCHC 2 cut(s) 177, 621
SetI ASST 5 cut(s) 381, 406, 418, 540, 711
SfaNI GCATC 3 cut(s) 109, 276, 579
Sfr303I CCGCGG 1 cut(s) 480
SgrBI CCGCGG 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 726
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 2 cut(s) 222, 625
SseBI AGGCCT 1 cut(s) 694
SsiI CCGC 6 cut(s) 267, 349, 398, 477, 479, 581
SspMI CTAG 4 cut(s) 230, 413, 614, 696
StuI AGGCCT 1 cut(s) 694
StyD4I CCNGG 2 cut(s) 156, 560
TaaI ACNGT 2 cut(s) 106, 504
TaqI TCGA 2 cut(s) 391, 528
TasI AATT 2 cut(s) 222, 625
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 2 cut(s) 139, 545
Tru1I TTAA 2 cut(s) 467, 506
Tru9I TTAA 2 cut(s) 467, 506
TscAI CASTG 1 cut(s) 733
TseFI GTSAC 2 cut(s) 100, 274
TseI GCWGC 3 cut(s) 416, 419, 685
Tsp45I GTSAC 2 cut(s) 100, 274
TspDTI ATGAA 1 cut(s) 358
TspRI CASTG 1 cut(s) 733
XapI RAATTY 1 cut(s) 625
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 4 cut(s) 230, 413, 614, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.