Rh6AG376600

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010338
Physical Location & Seq
Reverse (-)
57534954 .. 57536966
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG376600.1

Sequence Viewer

Length: 804 bp
ATGCAGTTATACCACCACCCGTATTCAATAGATAGCCAAAGGGTGAGACTCGCTTTGGAAGAAAAAGGCATTGATTACACATCATTCCATCTCAATCCTGTAACGGGCAAGAATATGAATGCCTCATTCTTCAGAATGAATCCAAGTGCAACACTCCCGGTTTTCCAGAACGGCGACCACAAGATTTACAATACAATTGAAATTATTCAGTATGTAGAAAGAATTGCAAGTGTCTCTACAGGCGTGGAGAACATGACCTTCAGTGGCAGAGAAGTCACTGAATGGATGCACCGAATACAACAATGGAACCCCAAGTTTTTCACACTTGCCCACATCCCGGACAAGTACTTTCGTTCTGTCTCCAAATTCTTGAGGCGGGTGGTGATGGCTCGAATGGAGGAATCTCCTGATTTAGCAGGTGCTTATCACAGAAAGTTGCAAGAAGTATATGACACAGAAGACAAGTTGAAAAACTCAAGTGTTTTGATACAGGACAAAGAACATCTAATAAGACTCCTTGATGAAGTTGAAAAACAGCTAAATGAAACAACATATTTAGCAGGGGAAGAGTTTACAATGGCAGATGTCATGCTCATTCCGGTGCTAGCACGGTTGGTGCTCTTGAATTTGGAGGACGAGTATATAGGTAGCCGGCCAAACACTGCTAAATACTGGATTATGGTACAGCAGAGGCCTAGTTATAAGAAAGTGATTGCGAGTCATTTCAATGGCTGGAGGAAGTATAGAACTTTGTGGAAAACCTGGTGCTCTATCCACATCAGAAGCATTCTAAGGAGATTTTGA

Protein Analysis

267

Amino Acids

31.63

Weight (kDa)

9.25

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 74 4.7e-11 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 3 - 76 1e-10 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 74 3.8e-10 Glutathione S-transferase, N-terminal domain
GST_C_3 PF14497 149 - 240 3.5e-07 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 166 - 210 1.2e-08 Glutathione S-transferase, C-terminal domain
GST_C PF00043 166 - 232 8.1e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AarI CACCTGC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 407
AciI CCGC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 653
AcsI RAATTY 2 cut(s) 365, 625
AcuI CTGAAG 2 cut(s) 115, 244
AfaI GTAC 2 cut(s) 347, 684
AfiI CCNNNNNNNGG 2 cut(s) 104, 337
AgsI TTSAA 6 cut(s) 27, 200, 469, 530, 625, 727
AjnI CCWGG 1 cut(s) 761
AluBI AGCT 1 cut(s) 538
AluI AGCT 1 cut(s) 538
Alw21I GWGCWC 2 cut(s) 621, 770
Alw26I GTCTC 3 cut(s) 40, 238, 364
AoxI GGCC 2 cut(s) 653, 692
ApoI RAATTY 2 cut(s) 365, 625
Asp700I GAANNNNTTC 1 cut(s) 204
AsuC2I CCSGG 2 cut(s) 158, 338
AsuHPI GGTGA 2 cut(s) 55, 394
AsuNHI GCTAGC 1 cut(s) 604
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 2 cut(s) 621, 770
BccI CCATC 2 cut(s) 96, 379
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 763
BcnI CCSGG 2 cut(s) 158, 338
BcoDI GTCTC 3 cut(s) 40, 238, 364
BfaI CTAG 2 cut(s) 605, 696
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 407
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 3 cut(s) 158, 338, 763
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 3 cut(s) 158, 338, 763
BmsI GCATC 1 cut(s) 276
BmtI GCTAGC 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 754
BpuEI CTTGAG 2 cut(s) 391, 460
BpuMI CCSGG 2 cut(s) 158, 338
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 337
Bse118I RCCGGY 1 cut(s) 651
Bse1I ACTGG 1 cut(s) 677
BseBI CCWGG 1 cut(s) 763
BseGI GGATG 2 cut(s) 291, 333
BseLI CCNNNNNNNGG 2 cut(s) 104, 337
BseNI ACTGG 1 cut(s) 677
BshFI GGCC 2 cut(s) 655, 694
BsiHKAI GWGCWC 2 cut(s) 621, 770
BsiSI CCGG 4 cut(s) 158, 338, 599, 652
BslI CCNNNNNNNGG 2 cut(s) 104, 337
BsmAI GTCTC 3 cut(s) 40, 238, 364
BsmI GAATGC 2 cut(s) 124, 786
BsnI GGCC 2 cut(s) 655, 694
Bsp1286I GDGCHC 2 cut(s) 621, 770
BspACI CCGC 1 cut(s) 376
BspANI GGCC 2 cut(s) 655, 694
BspLI GGNNCC 1 cut(s) 308
BspMI ACCTGC 1 cut(s) 407
BspOI GCTAGC 1 cut(s) 608
BsrFI RCCGGY 1 cut(s) 651
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 651
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 1 cut(s) 612
Bst6I CTCTTC 1 cut(s) 561
BstC8I GCNNGC 2 cut(s) 606, 653
BstDEI CTNAG 1 cut(s) 791
BstF5I GGATG 2 cut(s) 291, 333
BstMAI GTCTC 3 cut(s) 40, 238, 364
BstNI CCWGG 1 cut(s) 763
BstSCI CCNGG 3 cut(s) 156, 336, 761
BstSFI CTRYAG 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 465
BsuRI GGCC 2 cut(s) 655, 694
BtsCI GGATG 2 cut(s) 291, 333
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 3 cut(s) 268, 276, 660
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 2 cut(s) 606, 653
Cfr10I RCCGGY 1 cut(s) 651
CsiI ACCWGGT 1 cut(s) 761
Csp6I GTAC 2 cut(s) 346, 683
CviAII CATG 2 cut(s) 253, 589
CviJI RGCY 7 cut(s) 36, 389, 538, 651, 655, 694, 732
CviKI_1 RGCY 7 cut(s) 36, 389, 538, 651, 655, 694, 732
CviQI GTAC 2 cut(s) 346, 683
DdeI CTNAG 1 cut(s) 791
EaeI YGGCCR 1 cut(s) 653
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
Eco147I AGGCCT 1 cut(s) 694
Eco57I CTGAAG 2 cut(s) 115, 244
EcoRII CCWGG 1 cut(s) 761
FaeI CATG 2 cut(s) 256, 592
FatI CATG 2 cut(s) 252, 588
FauI CCCGC 1 cut(s) 369
FokI GGATG 2 cut(s) 298, 320
FspBI CTAG 2 cut(s) 605, 696
GsuI CTGGAG 1 cut(s) 754
HaeIII GGCC 2 cut(s) 655, 694
HapII CCGG 4 cut(s) 158, 338, 599, 652
Hin1II CATG 2 cut(s) 256, 592
HinfI GANTC 5 cut(s) 48, 139, 401, 513, 718
HpaII CCGG 4 cut(s) 158, 338, 599, 652
HphI GGTGA 2 cut(s) 55, 394
Hpy166II GTNNAC 1 cut(s) 573
Hpy188I TCNGA 2 cut(s) 134, 782
Hpy188III TCNNGA 4 cut(s) 166, 370, 407, 622
Hpy8I GTNNAC 1 cut(s) 573
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 1 cut(s) 612
HpyCH4V TGCA 5 cut(s) 4, 149, 227, 289, 439
HpyF3I CTNAG 1 cut(s) 791
Hsp92II CATG 2 cut(s) 256, 592
KroI GCCGGC 1 cut(s) 651
KroNI GCCGGC 1 cut(s) 653
LweI GCATC 1 cut(s) 276
MabI ACCWGGT 1 cut(s) 761
MaeI CTAG 2 cut(s) 605, 696
MaeIII GTNAC 2 cut(s) 100, 274
MboII GAAGA 4 cut(s) 71, 121, 470, 578
MfeI CAATTG 1 cut(s) 195
MhlI GDGCHC 2 cut(s) 621, 770
MluCI AATT 5 cut(s) 195, 201, 222, 365, 625
MlyI GAGTC 3 cut(s) 42, 507, 727
MnlI CCTC 6 cut(s) 133, 366, 391, 625, 684, 729
MroNI GCCGGC 1 cut(s) 651
MroXI GAANNNNTTC 1 cut(s) 204
MslI CAYNNNNRTG 2 cut(s) 599, 726
MspI CCGG 4 cut(s) 158, 338, 599, 652
MspR9I CCNGG 3 cut(s) 158, 338, 763
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 2 cut(s) 124, 786
MvaI CCWGG 1 cut(s) 763
NaeI GCCGGC 1 cut(s) 653
NciI CCSGG 2 cut(s) 158, 338
NgoMIV GCCGGC 1 cut(s) 651
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 2 cut(s) 256, 592
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 1 cut(s) 274
PaqCI CACCTGC 1 cut(s) 407
PceI AGGCCT 1 cut(s) 694
PctI GAATGC 2 cut(s) 124, 786
PdiI GCCGGC 1 cut(s) 653
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 2 cut(s) 139, 401
PfoI TCCNGGA 1 cut(s) 336
PleI GAGTC 3 cut(s) 42, 507, 726
PpsI GAGTC 3 cut(s) 42, 507, 726
PsiI TTATAA 1 cut(s) 702
Psp6I CCWGG 1 cut(s) 761
PspGI CCWGG 1 cut(s) 761
PspN4I GGNNCC 1 cut(s) 308
RsaI GTAC 2 cut(s) 347, 684
RsaNI GTAC 2 cut(s) 346, 683
RseI CAYNNNNRTG 2 cut(s) 599, 726
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 3 cut(s) 42, 507, 727
ScrFI CCNGG 3 cut(s) 158, 338, 763
SduI GDGCHC 2 cut(s) 621, 770
SetI ASST 5 cut(s) 260, 421, 540, 649, 764
SexAI ACCWGGT 1 cut(s) 761
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 599, 726
SmlI CTYRAG 2 cut(s) 370, 475
SmoI CTYRAG 2 cut(s) 370, 475
Sse9I AATT 5 cut(s) 195, 201, 222, 365, 625
SseBI AGGCCT 1 cut(s) 694
SsiI CCGC 1 cut(s) 376
SspMI CTAG 2 cut(s) 605, 696
StuI AGGCCT 1 cut(s) 694
StyD4I CCNGG 3 cut(s) 156, 336, 761
TaaI ACNGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 391
TasI AATT 5 cut(s) 195, 201, 222, 365, 625
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 2 cut(s) 139, 401
TscAI CASTG 3 cut(s) 268, 283, 667
TseFI GTSAC 1 cut(s) 274
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 4 cut(s) 131, 152, 537, 558
TspRI CASTG 3 cut(s) 268, 283, 667
XapI RAATTY 2 cut(s) 365, 625
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 605, 696
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.