AT1G77360

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009496
Physical Location & Seq
Reverse (-)
29071983 .. 29073729
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77360.1

Sequence Viewer

Length: 1554 bp
ATGAAGCGTTTTAGGATTAGATCAGTTGATTTTCGTCAGCTGGTTAATTTTTTTTCTTTTATGCGATGGGAATGTAGTTCTTCTGCTACGGTCTGGGTTAGATTCAACATGACGATCAGAATTATCAATCGTCAATCCAGATTCTGCTGTAAGAGTTTTCTGTCTGCGAGGCTGTATAGTTCAAGCGAACAAGTGAGAGATGTTGCGGATGTGGCGAAGAACATATCTAAAGTATTGATGTCTTCTCCGCAATTGGTGCTTGACTCGGCTTTAGATCAAAGTGGGTTAAGGGTTTCACAAGAAGTTGTAGAAGATGTTTTAAATAGATTCAGGAATGCTGGTTTGTTGACATACAGGTTCTTCCAATGGTCTGAGAAGCAACGGCATTACGAGCATAGTGTTCGTGCGTACCACATGATGATTGAGTCTACGGCGAAGATAAGGCAGTACAAGCTCATGTGGGATCTTATCAATGCAATGAGGAAGAAGAAGATGCTAAATGTAGAGACTTTTTGTATCGTGATGAGGAAGTATGCGAGGGCGCAGAAAGTGGATGAGGCGATTTACGCGTTTAATGTGATGGAGAAGTATGATTTGCCGCCGAATCTTGTGGCGTTCAACGGGTTGTTGAGTGCGTTGTGTAAATCCAAGAATGTGAGGAAAGCACAGGAAGTTTTCGAAAATATGAGGGATCGCTTTACGCCTGACTCCAAAACGTATAGCATATTGCTTGAGGGATGGGGGAAAGAACCCAACTTGCCCAAGGCAAGAGAGGTCTTCCGGGAAATGATTGATGCGGGTTGTCATCCGGATATAGTGACGTATAGTATAATGGTTGACATACTTTGTAAAGCTGGAAGAGTTGATGAAGCTCTTGGCATTGTGAGGAGCATGGATCCAAGCATTTGCAAGCCGACCACTTTTATCTATAGTGTGCTTGTACATACTTATGGAACTGAGAACCGGCTTGAAGAGGCGGTTGATACTTTCCTCGAGATGGAAAGAAGTGGAATGAAAGCCGATGTAGCAGTTTTCAATTCATTGATCGGTGCATTTTGCAAAGCAAATAGGATGAAGAATGTGTATAGGGTTTTGAAAGAAATGAAGAGTAAAGGTGTGACGCCTAACTCCAAGTCATGTAACATCATTCTACGCCATCTAATAGAACGAGGGGAGAAAGACGAAGCTTTTGATGTCTTTAGGAAGATGATTAAAGTGTGTGAACCAGATGCAGACACTTACACGATGGTGATCAAGATGTTTTGTGAAAAGAAAGAGATGGAGACAGCGGATAAAGTGTGGAAATACATGAGGAAGAAAGGGGTGTTCCCTAGTATGCATACATTCTCGGTTCTTATTAATGGGTTGTGCGAGGAGCGTACCACTCAGAAAGCTTGTGTTTTGTTAGAGGAGATGATCGAGATGGGGATTCGACCATCGGGTGTAACGTTTGGTAGATTAAGACAGCTGCTTATCAAAGAAGAGAGAGAAGATGTGCTCAAGTTCCTTAATGAGAAGATGAACGTTTTGGTCAATGAGCCGTTGTGTGATTGA

Protein Analysis

517

Amino Acids

60.2

Weight (kDa)

9.2

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 167 - 215 1.1e-07 PPR repeat family
PPR_3 PF13812 194 - 249 5.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 199 - 230 7.3e-07 PPR repeat
PPR_2 PF13041 201 - 246 4e-11 PPR repeat family
PPR_2 PF13041 235 - 284 3.5e-12 PPR repeat family
PPR_long PF17177 253 - 383 4.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 259 - 319 7.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 267 - 298 4.6e-12 PPR repeat
PPR_3 PF13812 332 - 385 6.8e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 337 - 403 1.9e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 337 - 369 8.2e-07 PPR repeat
PPR_2 PF13041 341 - 386 1.5e-12 PPR repeat family
PPR PF01535 344 - 373 1.6e-06 PPR repeat
PPR_2 PF13041 377 - 422 9.7e-10 PPR repeat family
PPR_long PF17177 394 - 485 1.5e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 408 - 454 9.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 409 - 457 1.1e-15 PPR repeat family
PPR_1 PF12854 440 - 472 1.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 428
AccII CGCG 1 cut(s) 569
AccIII TCCGGA 1 cut(s) 808
AciI CCGC 6 cut(s) 206, 248, 599, 797, 977, 1289
AclI AACGTT 2 cut(s) 1448, 1524
AclWI GGATC 4 cut(s) 471, 699, 890, 903
AcyI GRCGYC 1 cut(s) 1121
AfaI GTAC 4 cut(s) 410, 449, 942, 1381
AfiI CCNNNNNNNGG 1 cut(s) 997
AflIII ACRYGT 1 cut(s) 567
AgsI TTSAA 6 cut(s) 106, 183, 619, 971, 1036, 1096
AleI CACNNNNGTG 1 cut(s) 1247
AluBI AGCT 7 cut(s) 40, 454, 854, 872, 1187, 1394, 1468
AluI AGCT 7 cut(s) 40, 454, 854, 872, 1187, 1394, 1468
Alw21I GWGCWC 1 cut(s) 1500
Alw26I GTCTC 2 cut(s) 500, 1277
AlwI GGATC 4 cut(s) 471, 699, 890, 903
AlwNI CAGNNNCTG 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 992
Aor13HI TCCGGA 1 cut(s) 808
ApeKI GCWGC 1 cut(s) 1468
ArsI GACNNNNNNTTYG 2 cut(s) 1172, 1204
AseI ATTAAT 1 cut(s) 1359
AspLEI GCGC 1 cut(s) 544
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 1 cut(s) 1261
AsuII TTCGAA 1 cut(s) 678
AvaI CYCGRG 1 cut(s) 992
BamHI GGATCC 1 cut(s) 895
BbsI GAAGAC 2 cut(s) 234, 769
Bbv12I GWGCWC 1 cut(s) 1500
BbvI GCAGC 1 cut(s) 1455
BccI CCATC 9 cut(s) 60, 574, 732, 991, 1164, 1240, 1273, 1417, 1444
BceAI ACGGC 3 cut(s) 398, 447, 1525
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BclI TGATCA 1 cut(s) 1251
BcnI CCSGG 1 cut(s) 782
BcoDI GTCTC 2 cut(s) 500, 1277
BfaI CTAG 1 cut(s) 1332
BfmI CTRYAG 1 cut(s) 928
BisI GCNGC 2 cut(s) 599, 1469
BlsI GCNGC 2 cut(s) 600, 1470
Bme1390I CCNGG 1 cut(s) 782
BmeT110I CYCGRG 1 cut(s) 992
BmiI GGNNCC 1 cut(s) 897
BmrFI CCNGG 1 cut(s) 782
BmsI GCATC 3 cut(s) 483, 784, 1219
BpiI GAAGAC 2 cut(s) 234, 769
Bpu14I TTCGAA 1 cut(s) 678
BpuEI CTTGAG 2 cut(s) 752, 1484
BpuMI CCSGG 1 cut(s) 782
BsaBI GATNNNNATC 1 cut(s) 1250
BsaHI GRCGYC 1 cut(s) 1121
BsaJI CCNNGG 1 cut(s) 762
BsaWI WCCGGW 1 cut(s) 808
Bsc4I CCNNNNNNNGG 1 cut(s) 997
Bse118I RCCGGY 1 cut(s) 963
Bse3DI GCAATG 1 cut(s) 483
Bse8I GATNNNNATC 1 cut(s) 1250
BseAI TCCGGA 1 cut(s) 808
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 5 cut(s) 214, 559, 743, 805, 1077
BseJI GATNNNNATC 1 cut(s) 1250
BseLI CCNNNNNNNGG 1 cut(s) 997
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 3 cut(s) 363, 948, 1400
BseRI GAGGAG 3 cut(s) 901, 1388, 1424
BseXI GCAGC 1 cut(s) 1455
Bsh1236I CGCG 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 1500
BsiHKCI CYCGRG 1 cut(s) 992
BsiSI CCGG 3 cut(s) 781, 809, 964
BslI CCNNNNNNNGG 1 cut(s) 997
BsmAI GTCTC 2 cut(s) 500, 1277
BsmI GAATGC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 992
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 1 cut(s) 1500
Bsp13I TCCGGA 1 cut(s) 808
Bsp1407I TGTACA 1 cut(s) 940
Bsp143I GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
BspACI CCGC 6 cut(s) 206, 248, 599, 797, 977, 1289
BspCNI CTCAG 3 cut(s) 364, 949, 1399
BspEI TCCGGA 1 cut(s) 808
BspFNI CGCG 1 cut(s) 569
BspLI GGNNCC 1 cut(s) 897
BspPI GGATC 4 cut(s) 471, 699, 890, 903
BspT104I TTCGAA 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 963
BsrGI TGTACA 1 cut(s) 940
BssAI RCCGGY 1 cut(s) 963
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
BssNI GRCGYC 1 cut(s) 1121
BssT1I CCWWGG 1 cut(s) 762
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 4 cut(s) 853, 966, 1100, 1476
BstACI GRCGYC 1 cut(s) 1121
BstAPI GCANNNNNTGC 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 940
BstBI TTCGAA 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 911
BstDEI CTNAG 3 cut(s) 372, 957, 1386
BstF5I GGATG 5 cut(s) 214, 559, 743, 805, 1077
BstFNI CGCG 1 cut(s) 569
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 9 cut(s) 23, 117, 277, 466, 694, 898, 1047, 1254, 1419
BstMAI GTCTC 2 cut(s) 500, 1277
BstMBI GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
BstMWI GCNNNNNNNGC 6 cut(s) 212, 256, 391, 451, 566, 1025
BstSCI CCNGG 1 cut(s) 780
BstSFI CTRYAG 1 cut(s) 928
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 1455
BstV2I GAAGAC 2 cut(s) 234, 769
BstX2I RGATCY 2 cut(s) 463, 895
BstYI RGATCY 2 cut(s) 463, 895
BtgZI GCGATG 1 cut(s) 79
BtsCI GGATG 5 cut(s) 214, 559, 743, 805, 1077
Cac8I GCNNGC 1 cut(s) 911
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 963
CseI GACGC 1 cut(s) 1129
Csp6I GTAC 4 cut(s) 409, 448, 941, 1380
CviAII CATG 6 cut(s) 109, 415, 457, 892, 1137, 1309
CviQI GTAC 4 cut(s) 409, 448, 941, 1380
DdeI CTNAG 3 cut(s) 372, 957, 1386
DpnI GATC 9 cut(s) 22, 116, 276, 465, 693, 897, 1046, 1253, 1418
DpnII GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
DraI TTTAAA 1 cut(s) 321
Eam1104I CTCTTC 4 cut(s) 853, 966, 1100, 1476
EarI CTCTTC 4 cut(s) 853, 966, 1100, 1476
Eco130I CCWWGG 1 cut(s) 762
Eco88I CYCGRG 1 cut(s) 992
EcoT14I CCWWGG 1 cut(s) 762
EcoT22I ATGCAT 1 cut(s) 1341
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 6 cut(s) 112, 418, 460, 895, 1140, 1312
FatI CATG 6 cut(s) 108, 414, 456, 891, 1136, 1308
FauI CCCGC 1 cut(s) 790
FbaI TGATCA 1 cut(s) 1251
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 2 cut(s) 599, 1469
FokI GGATG 5 cut(s) 221, 566, 750, 792, 1084
Fsp4HI GCNGC 2 cut(s) 599, 1469
FspBI CTAG 1 cut(s) 1332
GlaI GCGC 1 cut(s) 543
GluI GCNGC 2 cut(s) 599, 1469
HapII CCGG 3 cut(s) 781, 809, 964
HgaI GACGC 1 cut(s) 1129
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 1 cut(s) 1121
Hin1II CATG 6 cut(s) 112, 418, 460, 895, 1140, 1312
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 2 cut(s) 348, 838
HindII GTYRAC 2 cut(s) 348, 838
HindIII AAGCTT 2 cut(s) 1185, 1392
HinfI GANTC 8 cut(s) 102, 141, 263, 327, 425, 604, 707, 1429
HpaII CCGG 3 cut(s) 781, 809, 964
HphI GGTGA 1 cut(s) 1261
Hpy166II GTNNAC 4 cut(s) 348, 429, 838, 1223
Hpy188I TCNGA 3 cut(s) 119, 373, 1389
Hpy188III TCNNGA 7 cut(s) 138, 331, 520, 809, 994, 1255, 1420
Hpy8I GTNNAC 4 cut(s) 348, 429, 838, 1223
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 4 cut(s) 716, 821, 1448, 1524
HpyCH4V TGCA 6 cut(s) 476, 909, 1052, 1059, 1232, 1339
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 256, 391, 451, 566, 1025
HpyF3I CTNAG 3 cut(s) 372, 957, 1386
HpySE526I ACGT 4 cut(s) 716, 821, 1448, 1524
Hsp92I GRCGYC 1 cut(s) 1121
Hsp92II CATG 6 cut(s) 112, 418, 460, 895, 1140, 1312
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 1251
Kzo9I GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
LmnI GCTCC 2 cut(s) 888, 1375
Lsp1109I GCAGC 1 cut(s) 1455
LweI GCATC 3 cut(s) 483, 784, 1219
MaeI CTAG 1 cut(s) 1332
MaeII ACGT 4 cut(s) 716, 821, 1448, 1524
MaeIII GTNAC 4 cut(s) 817, 1117, 1139, 1444
MalI GATC 9 cut(s) 22, 116, 276, 465, 693, 897, 1046, 1253, 1418
MboI GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
MfeI CAATTG 1 cut(s) 251
MflI RGATCY 2 cut(s) 463, 895
MhlI GDGCHC 1 cut(s) 1500
MluCI AATT 4 cut(s) 46, 120, 251, 1036
MluI ACGCGT 1 cut(s) 567
MlyI GAGTC 3 cut(s) 257, 434, 701
Mph1103I ATGCAT 1 cut(s) 1341
MroI TCCGGA 1 cut(s) 808
MseI TTAA 8 cut(s) 45, 287, 320, 573, 1212, 1359, 1460, 1509
MslI CAYNNNNRTG 2 cut(s) 948, 1247
MspA1I CMGCKG 3 cut(s) 40, 1289, 1468
MspI CCGG 3 cut(s) 781, 809, 964
MspR9I CCNGG 1 cut(s) 782
MunI CAATTG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 340
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 6 cut(s) 212, 256, 391, 451, 566, 1025
NciI CCSGG 1 cut(s) 782
NdeII GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
NlaIII CATG 6 cut(s) 112, 418, 460, 895, 1140, 1312
NlaIV GGNNCC 1 cut(s) 897
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 2 cut(s) 817, 1117
NsiI ATGCAT 1 cut(s) 1341
NspV TTCGAA 1 cut(s) 678
OliI CACNNNNGTG 1 cut(s) 1247
PaeR7I CTCGAG 1 cut(s) 992
PcsI WCGNNNNNNNCGW 1 cut(s) 1445
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 5 cut(s) 102, 141, 327, 604, 1429
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 2 cut(s) 600, 1470
PleI GAGTC 3 cut(s) 257, 433, 701
PpsI GAGTC 3 cut(s) 257, 433, 701
PshBI ATTAAT 1 cut(s) 1359
Psp1406I AACGTT 2 cut(s) 1448, 1524
PspN4I GGNNCC 1 cut(s) 897
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 2 cut(s) 463, 895
PvuII CAGCTG 2 cut(s) 40, 1468
RsaI GTAC 4 cut(s) 410, 449, 942, 1381
RsaNI GTAC 4 cut(s) 409, 448, 941, 1380
RseI CAYNNNNRTG 2 cut(s) 948, 1247
SaqAI TTAA 8 cut(s) 45, 287, 320, 573, 1212, 1359, 1460, 1509
SatI GCNGC 2 cut(s) 599, 1469
Sau3AI GATC 9 cut(s) 20, 114, 274, 463, 691, 895, 1044, 1251, 1416
SchI GAGTC 3 cut(s) 257, 434, 701
ScrFI CCNGG 1 cut(s) 782
SduI GDGCHC 1 cut(s) 1500
SfaNI GCATC 3 cut(s) 483, 784, 1219
SfcI CTRYAG 1 cut(s) 928
Sfr274I CTCGAG 1 cut(s) 992
SfuI TTCGAA 1 cut(s) 678
SlaI CTCGAG 1 cut(s) 992
SmiMI CAYNNNNRTG 2 cut(s) 948, 1247
SmlI CTYRAG 3 cut(s) 731, 992, 1499
SmoI CTYRAG 3 cut(s) 731, 992, 1499
Sse9I AATT 4 cut(s) 46, 120, 251, 1036
SsiI CCGC 6 cut(s) 206, 248, 599, 797, 977, 1289
SspMI CTAG 1 cut(s) 1332
StyD4I CCNGG 1 cut(s) 780
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 4 cut(s) 719, 824, 1451, 1527
TaqI TCGA 4 cut(s) 678, 993, 1419, 1432
TasI AATT 4 cut(s) 46, 120, 251, 1036
TatI WGTACW 2 cut(s) 447, 940
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 5 cut(s) 102, 141, 327, 604, 1429
Tru1I TTAA 8 cut(s) 45, 287, 320, 573, 1212, 1359, 1460, 1509
Tru9I TTAA 8 cut(s) 45, 287, 320, 573, 1212, 1359, 1460, 1509
TseFI GTSAC 2 cut(s) 817, 1117
TseI GCWGC 1 cut(s) 1468
Tsp45I GTSAC 2 cut(s) 817, 1117
TspDTI ATGAA 7 cut(s) 17, 882, 1028, 1029, 1088, 1118, 1535
VspI ATTAAT 1 cut(s) 1359
XhoI CTCGAG 1 cut(s) 992
XmiI GTMKAC 1 cut(s) 428
XspI CTAG 1 cut(s) 1332
Zsp2I ATGCAT 1 cut(s) 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.