pycom05g01820

Pentatricopeptide repeat-containing protein At1g77360

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009496
Physical Location & Seq
Forward (+)
2178196 .. 2179641
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01820.1

Sequence Viewer

Length: 1446 bp
ATGGCCATAAAATTACAAAAATGTGGGGAAAGATTAGGTTTTGGGTTGATGAAATTGACCCGGAATTACTGTTCAACAGAACCCATGAGGCAATTTTCCGACCCAACAAAAAGAATTTGCAAAATCATGATGTCCTGCCCAACACTTGCTCTTGATACCGCCCTTGACCAAACCGGGATTCGAATTTCACCTGAAATGGTAGAGGAAGTTCTCATGAGATTCAACAATGCCGGTATGGTTGCATACCGGTTCTTTGAGTGGGCGGCAAAGCAGCGAAATTATTCGCATAGTGTTAAGGCTTACCATGCTATGATTGAATCTTTGGCCAAGATCAATCAGTACCAGATTATGTTGGATCTTGTGAACTCGATGAGGGCGAAGAGGATTCAGAATATGGAGACGTTTGGAATCATTATGAGGAAGTATGCTAGGGCACAGAAGGTTGAGGAAGCGCTTTATACATTTAATGTTATGGAGAAGTATGATTGTGCTCCGAATTTAGCAGCGTTCAATGGCCTGCTGAGTGCTCTGTGTAAGTCCAAGAATGTGAGGAAGGCTCAGGAGGTTTTTGATAAAATGAAGGATCGGTTTGAGCCAGACTCAAAGACCTATAGTATATTGATCGGCGGATGGGGGAAGGATCCGAATTTGCCTAAAGCGAGGGAGGTGTTCAGAGAAATGATTGATGCGGGTTGCAATCCTGATAAAGTGACTTATGGTATCATGGTTGATGTTCTTTGCAAGGCAGGGAGGGTGGATGAAGCTATTGATATTGTTCGGGGCATGGATAATGGTGGTTGTACACCGACGTCTTTTATTTATAGTGTTTTGGTGCATACGTATGGGGTTGAAAACAGGATTGAAGATGCTGTTGCTGCATTCTTGGAGATGGAAAGGAATGGAATAAGGGCTGATGTGGTTGTGTACAATGCCTTGATCGGCGCTTTTTGTAAGGCTAACAAGTTCAAGAACGTCTATAGGGTCTTAAACGATATGAATTCCAAAGGCGTTACTCCCAATTCGAGGACTTGCAATATCATTTTAAATAGTTTGATTGACCGTGAAGAAACTGAGGAGGCATTTAGTGTCTTCCGCAAAATGATCAAAGTATGTGAGCCAGATGCAGATACGTATACAATGATGATAAAGATGTTTTGTGAGAGAGATGAGTTGAAGATGACTCATAAAGTCTGGAAGTACATGAAGTTGAAGCAGTTTGTCCCAAGCATGCACACATATTCGGTCCTTATAAATGGATTTTGTGAGAAGGGAAATGCTTCAAAGGCTTGTGTCTTACTGGAAGAGATGATAGAGAAGGGGATTCGGCCAGCAGGTGTGACTTTTGGGAGATTAAGGCAGTTGCTGATAAAAGAAGGAAGAGAAGATGTACTCAAATTTTTAAACGAAAAAGTTAATCTTCTTGTCAAGGAGCCTTTATTCGATTGA

Protein Analysis

482

Amino Acids

55.01

Weight (kDa)

8.9

Isoelectric Point (pI)

28.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 114 - 194 3e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 133 - 179 3.8e-08 PPR repeat family
PPR_1 PF12854 162 - 194 4.3e-07 PPR repeat
PPR_2 PF13041 165 - 210 1.4e-11 PPR repeat family
PPR_2 PF13041 199 - 248 6.5e-12 PPR repeat family
PPR_long PF17177 218 - 347 6.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 231 - 262 8.4e-12 PPR repeat
TPR_24 PF23276 291 - 392 2.8e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 296 - 351 1.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 300 - 332 2.1e-06 PPR repeat
PPR_2 PF13041 305 - 351 8.7e-15 PPR repeat family
PPR_3 PF13812 327 - 387 6.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 340 - 386 4.3e-10 PPR repeat family
PPR_long PF17177 357 - 467 2.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 373 - 421 1.1e-12 PPR repeat family
PPR_1 PF12854 406 - 436 1e-08 PPR repeat
PPR PF01535 412 - 441 3.2e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1250
AarI CACCTGC 1 cut(s) 1322
AatII GACGTC 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 1322
AccI GTMKAC 1 cut(s) 1133
AciI CCGC 5 cut(s) 159, 263, 627, 689, 1093
AclWI GGATC 4 cut(s) 363, 591, 635, 648
AcoI YGGCCR 3 cut(s) 3, 324, 1325
AcsI RAATTY 6 cut(s) 114, 183, 496, 646, 997, 1394
AcyI GRCGYC 1 cut(s) 809
AfaI GTAC 5 cut(s) 341, 802, 926, 1199, 1389
AfeI AGCGCT 1 cut(s) 453
AfiI CCNNNNNNNGG 1 cut(s) 1023
AgeI ACCGGT 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AloI GAACNNNNNNTCC 2 cut(s) 55, 87
AluBI AGCT 1 cut(s) 764
AluI AGCT 1 cut(s) 764
Alw21I GWGCWC 2 cut(s) 493, 529
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 4 cut(s) 363, 591, 635, 648
AlwNI CAGNNNCTG 1 cut(s) 1363
Aor51HI AGCGCT 1 cut(s) 453
AoxI GGCC 4 cut(s) 3, 324, 514, 1325
ApeKI GCWGC 3 cut(s) 271, 503, 875
ApoI RAATTY 6 cut(s) 114, 183, 496, 646, 997, 1394
AsiGI ACCGGT 1 cut(s) 246
Asp700I GAANNNNTTC 2 cut(s) 280, 1276
AspLEI GCGC 2 cut(s) 454, 944
AspS9I GGNCC 1 cut(s) 1243
AsuC2I CCSGG 2 cut(s) 61, 175
AsuHPI GGTGA 1 cut(s) 180
AsuII TTCGAA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 1243
BaeGI GKGCMC 1 cut(s) 436
BalI TGGCCA 2 cut(s) 5, 326
BamHI GGATCC 1 cut(s) 640
BbsI GAAGAC 1 cut(s) 1081
Bbv12I GWGCWC 2 cut(s) 493, 529
BbvI GCAGC 3 cut(s) 283, 515, 862
BccI CCATC 2 cut(s) 624, 883
BclI TGATCA 1 cut(s) 1101
BcnI CCSGG 2 cut(s) 61, 175
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 2 cut(s) 610, 976
BfoI RGCGCY 2 cut(s) 455, 945
BfuAI ACCTGC 1 cut(s) 1322
BisI GCNGC 4 cut(s) 264, 272, 504, 876
BlsI GCNGC 4 cut(s) 265, 273, 505, 877
Bme1390I CCNGG 2 cut(s) 61, 175
Bme18I GGWCC 1 cut(s) 1243
BmgT120I GGNCC 1 cut(s) 1243
BmiI GGNNCC 2 cut(s) 642, 1431
BmrFI CCNGG 2 cut(s) 61, 175
BmsI GCATC 3 cut(s) 676, 856, 1111
BpiI GAAGAC 1 cut(s) 1081
BplI GAGNNNNNCTC 4 cut(s) 541, 573, 584, 616
Bpu10I CCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 181
BpuMI CCSGG 2 cut(s) 61, 175
BsaAI YACGTR 2 cut(s) 840, 1131
BsaHI GRCGYC 1 cut(s) 809
BsaWI WCCGGW 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 1023
Bse118I RCCGGY 2 cut(s) 230, 246
Bse1I ACTGG 1 cut(s) 1302
BseGI GGATG 2 cut(s) 635, 763
BseLI CCNNNNNNNGG 1 cut(s) 1023
BseMII CTCAG 3 cut(s) 512, 572, 1062
BseNI ACTGG 1 cut(s) 1302
BseRI GAGGAG 1 cut(s) 1088
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 3 cut(s) 283, 515, 862
BshFI GGCC 4 cut(s) 5, 326, 516, 1327
BshTI ACCGGT 1 cut(s) 246
BsiHKAI GWGCWC 2 cut(s) 493, 529
BsiSI CCGG 4 cut(s) 61, 174, 231, 247
BslFI GGGAC 1 cut(s) 1205
BslI CCNNNNNNNGG 1 cut(s) 1023
BsmAI GTCTC 1 cut(s) 392
BsmBI CGTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 1205
BsmI GAATGC 1 cut(s) 878
BsnI GGCC 4 cut(s) 5, 326, 516, 1327
Bsp119I TTCGAA 1 cut(s) 181
Bsp1286I GDGCHC 3 cut(s) 436, 493, 529
Bsp1407I TGTACA 2 cut(s) 800, 924
Bsp143I GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
BspACI CCGC 5 cut(s) 159, 263, 627, 689, 1093
BspANI GGCC 4 cut(s) 5, 326, 516, 1327
BspCNI CTCAG 3 cut(s) 513, 571, 1063
BspHI TCATGA 2 cut(s) 126, 213
BspLI GGNNCC 2 cut(s) 642, 1431
BspMI ACCTGC 1 cut(s) 1322
BspPI GGATC 4 cut(s) 363, 591, 635, 648
BspT104I TTCGAA 1 cut(s) 181
BsrFI RCCGGY 2 cut(s) 230, 246
BsrGI TGTACA 2 cut(s) 800, 924
BsrI ACTGG 1 cut(s) 1302
BssAI RCCGGY 2 cut(s) 230, 246
BssMI GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
BssNAI GTATAC 1 cut(s) 1134
BssNI GRCGYC 1 cut(s) 809
Bst1107I GTATAC 1 cut(s) 1134
Bst4CI ACNGT 2 cut(s) 71, 1061
Bst6I CTCTTC 3 cut(s) 374, 1296, 1372
BstACI GRCGYC 1 cut(s) 809
BstAUI TGTACA 2 cut(s) 800, 924
BstBAI YACGTR 2 cut(s) 840, 1131
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 3 cut(s) 518, 1229, 1329
BstDEI CTNAG 3 cut(s) 521, 558, 1071
BstF5I GGATG 2 cut(s) 635, 763
BstH2I RGCGCY 2 cut(s) 455, 945
BstHHI GCGC 2 cut(s) 454, 944
BstKTI GATC 7 cut(s) 333, 358, 586, 624, 643, 939, 1104
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
BstMWI GCNNNNNNNGC 3 cut(s) 305, 875, 1283
BstNSI RCATGY 1 cut(s) 1231
BstSCI CCNGG 2 cut(s) 59, 173
BstSFI CTRYAG 2 cut(s) 610, 976
BstSLI GKGCMC 1 cut(s) 436
BstSNI TACGTA 2 cut(s) 840, 1131
BstV1I GCAGC 3 cut(s) 283, 515, 862
BstV2I GAAGAC 1 cut(s) 1081
BstX2I RGATCY 2 cut(s) 355, 640
BstYI RGATCY 2 cut(s) 355, 640
BstZ17I GTATAC 1 cut(s) 1134
BsuRI GGCC 4 cut(s) 5, 326, 516, 1327
BtsCI GGATG 2 cut(s) 635, 763
BveI ACCTGC 1 cut(s) 1322
Cac8I GCNNGC 3 cut(s) 518, 1229, 1329
CaiI CAGNNNCTG 1 cut(s) 1363
CciI TCATGA 2 cut(s) 126, 213
CfoI GCGC 2 cut(s) 454, 944
Cfr10I RCCGGY 2 cut(s) 230, 246
Cfr13I GGNCC 1 cut(s) 1243
Csp6I GTAC 5 cut(s) 340, 801, 925, 1198, 1388
CspAI ACCGGT 1 cut(s) 246
CviAII CATG 8 cut(s) 85, 127, 214, 305, 724, 784, 1201, 1228
CviQI GTAC 5 cut(s) 340, 801, 925, 1198, 1388
DdeI CTNAG 3 cut(s) 521, 558, 1071
DpnI GATC 7 cut(s) 332, 357, 585, 623, 642, 938, 1103
DpnII GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
DraI TTTAAA 2 cut(s) 1044, 1401
EaeI YGGCCR 3 cut(s) 3, 324, 1325
Eam1104I CTCTTC 3 cut(s) 374, 1296, 1372
EarI CTCTTC 3 cut(s) 374, 1296, 1372
EciI GGCGGA 1 cut(s) 642
Eco105I TACGTA 2 cut(s) 840, 1131
Eco47I GGWCC 1 cut(s) 1243
Eco47III AGCGCT 1 cut(s) 453
EcoRI GAATTC 1 cut(s) 997
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 8 cut(s) 88, 130, 217, 308, 727, 787, 1204, 1231
FalI AAGNNNNNCTT 2 cut(s) 1401, 1433
FaqI GGGAC 1 cut(s) 1205
FatI CATG 8 cut(s) 84, 126, 213, 304, 723, 783, 1200, 1227
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 1 cut(s) 1101
FblI GTMKAC 1 cut(s) 1133
Fnu4HI GCNGC 4 cut(s) 264, 272, 504, 876
FokI GGATG 2 cut(s) 642, 770
Fsp4HI GCNGC 4 cut(s) 264, 272, 504, 876
FspBI CTAG 1 cut(s) 429
GlaI GCGC 2 cut(s) 453, 943
GluI GCNGC 4 cut(s) 264, 272, 504, 876
HaeII RGCGCY 2 cut(s) 455, 945
HaeIII GGCC 4 cut(s) 5, 326, 516, 1327
HapII CCGG 4 cut(s) 61, 174, 231, 247
HhaI GCGC 2 cut(s) 454, 944
Hin1I GRCGYC 1 cut(s) 809
Hin1II CATG 8 cut(s) 88, 130, 217, 308, 727, 787, 1204, 1231
Hin6I GCGC 2 cut(s) 452, 942
HinP1I GCGC 2 cut(s) 452, 942
HinfI GANTC 8 cut(s) 178, 219, 317, 385, 408, 599, 1180, 1321
HpaII CCGG 4 cut(s) 61, 174, 231, 247
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 4 cut(s) 364, 803, 925, 1134
Hpy188I TCNGA 5 cut(s) 100, 390, 495, 645, 674
Hpy188III TCNNGA 7 cut(s) 127, 152, 214, 560, 701, 967, 1192
Hpy8I GTNNAC 4 cut(s) 364, 803, 925, 1134
Hpy99I CGWCG 1 cut(s) 811
HpyAV CCTTC 7 cut(s) 433, 547, 574, 631, 1261, 1309, 1367
HpyCH4III ACNGT 2 cut(s) 71, 1061
HpyCH4IV ACGT 5 cut(s) 401, 809, 839, 972, 1130
HpyCH4V TGCA 9 cut(s) 120, 242, 696, 741, 835, 878, 1032, 1124, 1231
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 875, 1283
HpyF3I CTNAG 3 cut(s) 521, 558, 1071
HpySE526I ACGT 5 cut(s) 401, 809, 839, 972, 1130
Hsp92I GRCGYC 1 cut(s) 809
Hsp92II CATG 8 cut(s) 88, 130, 217, 308, 727, 787, 1204, 1231
HspAI GCGC 2 cut(s) 452, 942
Ksp22I TGATCA 1 cut(s) 1101
Kzo9I GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
LmnI GCTCC 2 cut(s) 496, 1429
Lsp1109I GCAGC 3 cut(s) 283, 515, 862
LweI GCATC 3 cut(s) 676, 856, 1111
MaeI CTAG 1 cut(s) 429
MaeII ACGT 5 cut(s) 401, 809, 839, 972, 1130
MaeIII GTNAC 3 cut(s) 709, 1009, 1336
MalI GATC 7 cut(s) 332, 357, 585, 623, 642, 938, 1103
MboI GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
MboII GAAGA 9 cut(s) 391, 875, 1076, 1081, 1186, 1313, 1389, 1394, 1409
MflI RGATCY 2 cut(s) 355, 640
MhlI GDGCHC 3 cut(s) 436, 493, 529
MlsI TGGCCA 2 cut(s) 5, 326
MluNI TGGCCA 2 cut(s) 5, 326
MlyI GAGTC 2 cut(s) 593, 1174
MmeI TCCRAC 2 cut(s) 123, 333
Mox20I TGGCCA 2 cut(s) 5, 326
MroXI GAANNNNTTC 2 cut(s) 280, 1276
MscI TGGCCA 2 cut(s) 5, 326
MseI TTAA 7 cut(s) 294, 465, 986, 1043, 1352, 1400, 1413
MslI CAYNNNNRTG 1 cut(s) 840
Msp20I TGGCCA 2 cut(s) 5, 326
MspI CCGG 4 cut(s) 61, 174, 231, 247
MspR9I CCNGG 2 cut(s) 61, 175
Mva1269I GAATGC 1 cut(s) 878
MwoI GCNNNNNNNGC 3 cut(s) 305, 875, 1283
NciI CCSGG 2 cut(s) 61, 175
NdeII GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
NlaIII CATG 8 cut(s) 88, 130, 217, 308, 727, 787, 1204, 1231
NlaIV GGNNCC 2 cut(s) 642, 1431
NmuCI GTSAC 2 cut(s) 709, 1336
NspI RCATGY 1 cut(s) 1231
NspV TTCGAA 1 cut(s) 181
PaeI GCATGC 1 cut(s) 1231
PagI TCATGA 2 cut(s) 126, 213
PaqCI CACCTGC 1 cut(s) 1322
PcsI WCGNNNNNNNCGW 1 cut(s) 374
PctI GAATGC 1 cut(s) 878
PdmI GAANNNNTTC 2 cut(s) 280, 1276
PfeI GAWTC 6 cut(s) 178, 219, 317, 385, 408, 1321
PinAI ACCGGT 1 cut(s) 246
PkrI GCNGC 4 cut(s) 265, 273, 505, 877
PleI GAGTC 2 cut(s) 593, 1174
PpsI GAGTC 2 cut(s) 593, 1174
Ppu21I YACGTR 2 cut(s) 840, 1131
PsiI TTATAA 1 cut(s) 1250
PspN4I GGNNCC 2 cut(s) 642, 1431
PspPI GGNCC 1 cut(s) 1243
PsrI GAACNNNNNNTAC 2 cut(s) 192, 224
PstNI CAGNNNCTG 1 cut(s) 1363
PsuI RGATCY 2 cut(s) 355, 640
RsaI GTAC 5 cut(s) 341, 802, 926, 1199, 1389
RsaNI GTAC 5 cut(s) 340, 801, 925, 1198, 1388
RseI CAYNNNNRTG 1 cut(s) 840
SaqAI TTAA 7 cut(s) 294, 465, 986, 1043, 1352, 1400, 1413
SatI GCNGC 4 cut(s) 264, 272, 504, 876
Sau3AI GATC 7 cut(s) 330, 355, 583, 621, 640, 936, 1101
Sau96I GGNCC 1 cut(s) 1243
SchI GAGTC 2 cut(s) 593, 1174
ScrFI CCNGG 2 cut(s) 61, 175
SduI GDGCHC 3 cut(s) 436, 493, 529
SfaNI GCATC 3 cut(s) 676, 856, 1111
SfcI CTRYAG 2 cut(s) 610, 976
SfuI TTCGAA 1 cut(s) 181
SinI GGWCC 1 cut(s) 1243
SmiMI CAYNNNNRTG 1 cut(s) 840
SnaBI TACGTA 2 cut(s) 840, 1131
SphI GCATGC 1 cut(s) 1231
SsiI CCGC 5 cut(s) 159, 263, 627, 689, 1093
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 59, 173
TaaI ACNGT 2 cut(s) 71, 1061
TaiI ACGT 5 cut(s) 404, 812, 842, 975, 1133
TaqI TCGA 4 cut(s) 181, 368, 1022, 1440
TaqII GACCGA 1 cut(s) 1231
TatI WGTACW 4 cut(s) 800, 924, 1197, 1387
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 6 cut(s) 178, 219, 317, 385, 408, 1321
Tru1I TTAA 7 cut(s) 294, 465, 986, 1043, 1352, 1400, 1413
Tru9I TTAA 7 cut(s) 294, 465, 986, 1043, 1352, 1400, 1413
TseFI GTSAC 2 cut(s) 709, 1336
TseI GCWGC 3 cut(s) 271, 503, 875
Tsp45I GTSAC 2 cut(s) 709, 1336
TspDTI ATGAA 5 cut(s) 65, 593, 774, 1010, 1217
VpaK11BI GGWCC 1 cut(s) 1243
XapI RAATTY 6 cut(s) 114, 183, 496, 646, 997, 1394
XceI RCATGY 1 cut(s) 1231
XmiI GTMKAC 1 cut(s) 1133
XmnI GAANNNNTTC 2 cut(s) 280, 1276
XspI CTAG 1 cut(s) 429
ZraI GACGTC 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.