pycom05g01730

Pentatricopeptide repeat-containing protein At1g77360

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009496
Physical Location & Seq
Forward (+)
2122386 .. 2123447
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01730.1

Sequence Viewer

Length: 1062 bp
ATGCAGAATATGGAGACGTTTGGAATCATTATGAGGAAGTATGCCAGGGCACAGAAAGTTGAGGAAGCGCTTTATACGTTTAATGTTATGGAGAAGTATGATTGTGCCCCAAATTTAGCAGCGTTCAATGACCTGCTGAGTGCTCTGTGTAAGTCCAAGAATGTGAGGAAGGCTCAGGAGGTTTTTGATAAAATGAAGGATCGGTTTCAGCCAGACTCAAAGACCTATAGTATATTGATTGGCGGATGGGGGAAGGATCCGAATTTGCCTAAAGCGAGGCAGGTGTTCAGAGAAATGAATGATGCAGGTTGCAATCCTGATATAGTGACTTATGGTATCATGGTTGATGTTCTTTGCAAGGCAGGGAGGGTGGATGAAGCCGTTGATATTGTTCGGGGCATGGATGATAGTGGTTGTACACCGACGTCTTTTATTTATAGTGTTTTGGTGCATACGTATGGGGTTGAAAACAGGATTGAAGATGCTGTTGCTGCATTCTTGGAGATGGAAAGGAATGGAATAAGGGCTGATGTGGTTGTGTACAATGCCTTGATCGGCGCTTTTTGTAAGGCTAACAAGTTCAAGAACGTCTATAGGGTCTTAAACGATATGAATTCCAAAGGCGTTACTCCCAATTCAAGGACTTGCAATGTCATTTTAAATAGTTTGATTGATCGTGAAGAGACTGAGGAGGCATTTAGTGTCTTTCGCAAAATGATCAAAGGATGCGAACCAGATGCAGATACGTATACAATGATGATAAAGATGTTTTGTGAGAGAGATGAGTTGAAGATGGCTCATAAAGTCTGGAAGTACATGAAGTTGAAGCAGTTTGTCCCGAGCATGCACACATATTCAGTCCTTATAAATGGATATTGTGAGAAGGGAAATGCTTCAAGGGCTTGTGTCTTACTGGAAGAGATGGTAGAGAAGGGGATTCGGCCAGCGGGTGTGACTTTTGGGAGATTAAGGCAGTTGCTGATAAAAGAAGGAAGAGAAGATGTACTCAAATTTTTAAACGAAAAAGTTAATCTTCTTGTCAAGGAGCCTTTATTCGATTGA

Protein Analysis

354

Amino Acids

40.23

Weight (kDa)

8.18

Isoelectric Point (pI)

26.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 34 - 66 3.2e-06 PPR repeat
PPR_2 PF13041 37 - 82 1.6e-11 PPR repeat family
PPR_2 PF13041 71 - 120 3e-12 PPR repeat family
PPR_long PF17177 90 - 219 1.4e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 95 - 154 6.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 103 - 134 3.1e-12 PPR repeat
PPR PF01535 109 - 139 1.5e-06 PPR repeat
TPR_24 PF23276 164 - 271 1.7e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 168 - 223 5.7e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 172 - 204 1.4e-06 PPR repeat
PPR_2 PF13041 177 - 223 1.6e-14 PPR repeat family
PPR_3 PF13812 199 - 259 6.2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 215 - 258 2.1e-11 PPR repeat family
PPR_long PF17177 229 - 339 1.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 237 - 292 8.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 241 - 274 5e-07 PPR repeat
PPR_2 PF13041 253 - 293 7.3e-11 PPR repeat family
PPR_1 PF12854 278 - 309 7.8e-09 PPR repeat
PPR PF01535 284 - 313 2.1e-07 PPR repeat
PPR_2 PF13041 288 - 320 4.8e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AarI CACCTGC 1 cut(s) 271
AatII GACGTC 1 cut(s) 428
Acc36I ACCTGC 3 cut(s) 141, 271, 296
AccI GTMKAC 1 cut(s) 749
AciI CCGC 2 cut(s) 243, 947
AclWI GGATC 3 cut(s) 207, 251, 264
AcoI YGGCCR 1 cut(s) 941
AcsI RAATTY 4 cut(s) 112, 262, 613, 1010
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 4 cut(s) 418, 542, 815, 1005
AfeI AGCGCT 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 639
AgsI TTSAA 8 cut(s) 127, 467, 479, 583, 639, 790, 826, 897
AjnI CCWGG 1 cut(s) 44
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 2 cut(s) 8, 677
AlwI GGATC 3 cut(s) 207, 251, 264
AlwNI CAGNNNCTG 1 cut(s) 979
Ama87I CYCGRG 1 cut(s) 838
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 1 cut(s) 941
ApeKI GCWGC 2 cut(s) 119, 491
ApoI RAATTY 4 cut(s) 112, 262, 613, 1010
Asp700I GAANNNNTTC 1 cut(s) 892
AspLEI GCGC 2 cut(s) 70, 560
AvaI CYCGRG 1 cut(s) 838
BaeGI GKGCMC 2 cut(s) 52, 109
BamHI GGATCC 1 cut(s) 256
Bbv12I GWGCWC 1 cut(s) 145
BbvI GCAGC 2 cut(s) 131, 478
BccI CCATC 4 cut(s) 240, 499, 787, 916
BceAI ACGGC 1 cut(s) 365
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BciT130I CCWGG 1 cut(s) 46
BclI TGATCA 1 cut(s) 717
BcoDI GTCTC 2 cut(s) 8, 677
BfmI CTRYAG 2 cut(s) 226, 592
BfoI RGCGCY 2 cut(s) 71, 561
BfuAI ACCTGC 3 cut(s) 141, 271, 296
BisI GCNGC 2 cut(s) 120, 492
BlsI GCNGC 2 cut(s) 121, 493
Bme1390I CCNGG 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 838
BmiI GGNNCC 2 cut(s) 258, 1047
BmrFI CCNGG 1 cut(s) 46
BmsI GCATC 4 cut(s) 292, 472, 716, 727
BplI GAGNNNNNCTC 2 cut(s) 157, 189
Bpu10I CCTNAGC 1 cut(s) 174
BsaAI YACGTR 2 cut(s) 456, 747
BsaHI GRCGYC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse1I ACTGG 1 cut(s) 918
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 1 cut(s) 46
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 4 cut(s) 251, 379, 409, 731
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 3 cut(s) 128, 188, 678
BseNI ACTGG 1 cut(s) 918
BseRI GAGGAG 1 cut(s) 704
BseSI GKGCMC 2 cut(s) 52, 109
BseXI GCAGC 2 cut(s) 131, 478
BshFI GGCC 1 cut(s) 943
BsiHKAI GWGCWC 1 cut(s) 145
BsiHKCI CYCGRG 1 cut(s) 838
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 1 cut(s) 639
BsmAI GTCTC 2 cut(s) 8, 677
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 821
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 1 cut(s) 943
BsoBI CYCGRG 1 cut(s) 838
Bsp1286I GDGCHC 3 cut(s) 52, 109, 145
Bsp1407I TGTACA 2 cut(s) 416, 540
Bsp143I GATC 5 cut(s) 199, 256, 552, 673, 717
BspACI CCGC 2 cut(s) 243, 947
BspANI GGCC 1 cut(s) 943
BspCNI CTCAG 3 cut(s) 129, 187, 679
BspLI GGNNCC 2 cut(s) 258, 1047
BspMI ACCTGC 3 cut(s) 141, 271, 296
BspPI GGATC 3 cut(s) 207, 251, 264
BsrDI GCAATG 1 cut(s) 655
BsrGI TGTACA 2 cut(s) 416, 540
BsrI ACTGG 1 cut(s) 918
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 5 cut(s) 199, 256, 552, 673, 717
BssNAI GTATAC 1 cut(s) 750
BssNI GRCGYC 1 cut(s) 425
Bst1107I GTATAC 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 46
Bst6I CTCTTC 3 cut(s) 675, 912, 988
BstACI GRCGYC 1 cut(s) 425
BstAUI TGTACA 2 cut(s) 416, 540
BstBAI YACGTR 2 cut(s) 456, 747
BstC8I GCNNGC 2 cut(s) 845, 945
BstDEI CTNAG 3 cut(s) 137, 174, 687
BstF5I GGATG 4 cut(s) 251, 379, 409, 731
BstH2I RGCGCY 2 cut(s) 71, 561
BstHHI GCGC 2 cut(s) 70, 560
BstKTI GATC 5 cut(s) 202, 259, 555, 676, 720
BstMAI GTCTC 2 cut(s) 8, 677
BstMBI GATC 5 cut(s) 199, 256, 552, 673, 717
BstMWI GCNNNNNNNGC 2 cut(s) 491, 899
BstNI CCWGG 1 cut(s) 46
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 2 cut(s) 226, 592
BstSLI GKGCMC 2 cut(s) 52, 109
BstSNI TACGTA 2 cut(s) 456, 747
BstV1I GCAGC 2 cut(s) 131, 478
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BstZ17I GTATAC 1 cut(s) 750
BsuRI GGCC 1 cut(s) 943
BtsCI GGATG 4 cut(s) 251, 379, 409, 731
BveI ACCTGC 3 cut(s) 141, 271, 296
Cac8I GCNNGC 2 cut(s) 845, 945
CaiI CAGNNNCTG 1 cut(s) 979
CfoI GCGC 2 cut(s) 70, 560
Csp6I GTAC 4 cut(s) 417, 541, 814, 1004
CviAII CATG 4 cut(s) 340, 400, 817, 844
CviJI RGCY 9 cut(s) 173, 211, 380, 527, 572, 797, 902, 943, 1048
CviKI_1 RGCY 9 cut(s) 173, 211, 380, 527, 572, 797, 902, 943, 1048
CviQI GTAC 4 cut(s) 417, 541, 814, 1004
DdeI CTNAG 3 cut(s) 137, 174, 687
DpnI GATC 5 cut(s) 201, 258, 554, 675, 719
DpnII GATC 5 cut(s) 199, 256, 552, 673, 717
DraI TTTAAA 2 cut(s) 660, 1017
EaeI YGGCCR 1 cut(s) 941
Eam1104I CTCTTC 3 cut(s) 675, 912, 988
EarI CTCTTC 3 cut(s) 675, 912, 988
EciI GGCGGA 1 cut(s) 258
Eco105I TACGTA 2 cut(s) 456, 747
Eco47III AGCGCT 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 838
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 4 cut(s) 343, 403, 820, 847
FalI AAGNNNNNCTT 2 cut(s) 1017, 1049
FaqI GGGAC 1 cut(s) 821
FatI CATG 4 cut(s) 339, 399, 816, 843
FauI CCCGC 1 cut(s) 940
FbaI TGATCA 1 cut(s) 717
FblI GTMKAC 1 cut(s) 749
Fnu4HI GCNGC 2 cut(s) 120, 492
FokI GGATG 4 cut(s) 258, 386, 416, 738
Fsp4HI GCNGC 2 cut(s) 120, 492
GlaI GCGC 2 cut(s) 69, 559
GluI GCNGC 2 cut(s) 120, 492
HaeII RGCGCY 2 cut(s) 71, 561
HaeIII GGCC 1 cut(s) 943
HhaI GCGC 2 cut(s) 70, 560
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 4 cut(s) 343, 403, 820, 847
Hin6I GCGC 2 cut(s) 68, 558
HinP1I GCGC 2 cut(s) 68, 558
HinfI GANTC 3 cut(s) 24, 215, 937
Hpy166II GTNNAC 3 cut(s) 419, 541, 750
Hpy188I TCNGA 2 cut(s) 261, 290
Hpy188III TCNNGA 6 cut(s) 176, 317, 583, 677, 808, 838
Hpy8I GTNNAC 3 cut(s) 419, 541, 750
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 6 cut(s) 163, 190, 247, 877, 925, 983
HpyCH4IV ACGT 6 cut(s) 17, 77, 425, 455, 588, 746
HpyCH4V TGCA 9 cut(s) 4, 305, 312, 357, 451, 494, 648, 740, 847
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 899
HpyF3I CTNAG 3 cut(s) 137, 174, 687
HpySE526I ACGT 6 cut(s) 17, 77, 425, 455, 588, 746
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 4 cut(s) 343, 403, 820, 847
HspAI GCGC 2 cut(s) 68, 558
Ksp22I TGATCA 1 cut(s) 717
Kzo9I GATC 5 cut(s) 199, 256, 552, 673, 717
LmnI GCTCC 1 cut(s) 1045
Lsp1109I GCAGC 2 cut(s) 131, 478
LweI GCATC 4 cut(s) 292, 472, 716, 727
MaeII ACGT 6 cut(s) 17, 77, 425, 455, 588, 746
MaeIII GTNAC 3 cut(s) 325, 625, 952
MalI GATC 5 cut(s) 201, 258, 554, 675, 719
MboI GATC 5 cut(s) 199, 256, 552, 673, 717
MboII GAAGA 7 cut(s) 491, 692, 802, 929, 1005, 1010, 1025
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 3 cut(s) 52, 109, 145
MluCI AATT 5 cut(s) 112, 262, 613, 634, 1010
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 8 cut(s) 27, 55, 159, 172, 270, 360, 682, 685
MroXI GAANNNNTTC 1 cut(s) 892
MseI TTAA 6 cut(s) 81, 602, 659, 968, 1016, 1029
MslI CAYNNNNRTG 1 cut(s) 456
MspA1I CMGCKG 1 cut(s) 947
MspR9I CCNGG 1 cut(s) 46
Mva1269I GAATGC 1 cut(s) 494
MvaI CCWGG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 491, 899
NdeII GATC 5 cut(s) 199, 256, 552, 673, 717
NlaIII CATG 4 cut(s) 343, 403, 820, 847
NlaIV GGNNCC 2 cut(s) 258, 1047
NmuCI GTSAC 2 cut(s) 325, 952
NspI RCATGY 1 cut(s) 847
PaeI GCATGC 1 cut(s) 847
PaqCI CACCTGC 1 cut(s) 271
PctI GAATGC 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 892
PfeI GAWTC 2 cut(s) 24, 937
PkrI GCNGC 2 cut(s) 121, 493
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
Ppu21I YACGTR 2 cut(s) 456, 747
PsiI TTATAA 1 cut(s) 866
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 258, 1047
PstNI CAGNNNCTG 1 cut(s) 979
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 4 cut(s) 418, 542, 815, 1005
RsaNI GTAC 4 cut(s) 417, 541, 814, 1004
RseI CAYNNNNRTG 1 cut(s) 456
SaqAI TTAA 6 cut(s) 81, 602, 659, 968, 1016, 1029
SatI GCNGC 2 cut(s) 120, 492
Sau3AI GATC 5 cut(s) 199, 256, 552, 673, 717
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 3 cut(s) 52, 109, 145
SfaNI GCATC 4 cut(s) 292, 472, 716, 727
SfcI CTRYAG 2 cut(s) 226, 592
SmiMI CAYNNNNRTG 1 cut(s) 456
SnaBI TACGTA 2 cut(s) 456, 747
SphI GCATGC 1 cut(s) 847
Sse9I AATT 5 cut(s) 112, 262, 613, 634, 1010
SsiI CCGC 2 cut(s) 243, 947
StyD4I CCNGG 1 cut(s) 44
TaiI ACGT 6 cut(s) 20, 80, 428, 458, 591, 749
TaqI TCGA 1 cut(s) 1056
TasI AATT 5 cut(s) 112, 262, 613, 634, 1010
TatI WGTACW 4 cut(s) 416, 540, 813, 1003
TfiI GAWTC 2 cut(s) 24, 937
Tru1I TTAA 6 cut(s) 81, 602, 659, 968, 1016, 1029
Tru9I TTAA 6 cut(s) 81, 602, 659, 968, 1016, 1029
TseFI GTSAC 2 cut(s) 325, 952
TseI GCWGC 2 cut(s) 119, 491
Tsp45I GTSAC 2 cut(s) 325, 952
TspDTI ATGAA 5 cut(s) 209, 311, 390, 626, 833
XapI RAATTY 4 cut(s) 112, 262, 613, 1010
XceI RCATGY 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 892
ZraI GACGTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.