Rh7AG295400

Pentatricopeptide repeat-containing protein At1g77360

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009496
Physical Location & Seq
Forward (+)
32325669 .. 32332526
6858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG295400.1

Sequence Viewer

Length: 1446 bp
ATGATTATCAAGATACAGAGCTATGGAGAAAAATTGGGTTTTCTATGGATGATGCTCTCCCGAAATTACTGTTCAAGTGAAGCAACAAGGCACATTACTGATCCAACCAAAAGAATATGCAAAATCATGATGTCTTGCCCCACACTCACACTTGATACTGCCCTTGATCAAAGTGGGGTCAGAATTTCACCTGAAATAGTAGAGGATGTTCTCGTGAGATTCAAGAATGCCGGAATGGTTGCATACCGCTTCTTTGAATGGGCAGCGAAGCAGCAAAACTACACGCATAGTGTTAAGGCATATCATGCTATGATCGATTCTTTGGCAAAGATAAGGCAGTACCAGATCATGTGGGAGCTTGTGAACTCAATGAGGACCCAAAGGATACAGAATATGGAGACATTTGGTATCATTATGAGGAAGTATGCCAGGGCTCAGAAGGTTGAGGAAGCGATTTATACTTTTAATGTTATGGAAAAGTATGATTGTGCTCCGAATTTAGCGGCATTCAATGGCCTACTGAGTGCTTTGTGCAAATCCAAGAATGTGAGGAAGGCTCAGGAGATTTTTGATAAAATGAAGGACCGCTTTGAGCCAGACTCAAAGACATACAGTATATTAATTGATGGATGGGGAAAAGATCCAAATTTGCCCAAGGCAAGGGAGGTCTTTAGAGAAATGGTTGATGCTGGCTGTAATCCTGATATAGTGACTTATGGTATCATGGTTGATGTTCTGTGCAAAGCAGGGAGGGTGGACGAAGCCATTGAGATTGTTCAGGGCATGGATGCTAGTGGTTGTATGCCTACATCTTTTATTTACAGTGTTCTGGTGCATACATATGGGGTGGAAAATAGGATTGAAGATGCTGTTGAAGCATTCTTGGAGATGGAAAGGAATGGAATCAAGGCTGATGTAGCCGTCTATAATGCCTTGATTGGTGCTTTTTGTAAAGTTAACAAGTTCAAGAACGTGTGGAGGGTCTTAAATGATATGACTTCCAAAGGTGTTACAGCCAATTCGAGGACCTGCAATATCATCTTAAACAGCTTGATTGACCGTGGAGAAACTGATGAGGCATTTAGTGTCTTTCGTAAAATGATCAAAGTATGTGATCCAGATGCAGATACATATACAATGATGATAAAAATGTTTTGCGAGAGAAATGAATTAGAGATGGCTCTTAAAGTGTGGAAGTGCATGAAGTTGAAACAGTTTGTTCCCAGTATGCATACATACTCAGTCCTTATTAATGGATTGTGTGAAAATGGTAATGCTTCAAAGGCTTGTGTCTTACTGGAAGAGATGATAGAGAAGGGGATTCGGCCATCAGGTGTGACATTTGGGAGATTAAGACAGTTGCTTATAAAAGAAGGAAGAGAAGATGTACTGAAATTTCTTCAAGAAAAAATCAATCTCCTCGTCAAAGAGCCTTTATCTGATTGA

Protein Analysis

481

Amino Acids

54.95

Weight (kDa)

8.37

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 133 - 179 3.8e-08 PPR repeat family
PPR_1 PF12854 162 - 194 1.3e-06 PPR repeat
PPR_2 PF13041 165 - 210 5.5e-12 PPR repeat family
PPR_2 PF13041 207 - 248 2.8e-09 PPR repeat family
TPR_24 PF23276 220 - 321 9.3e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 223 - 282 1.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 231 - 262 4.4e-12 PPR repeat
PPR PF01535 237 - 267 3.2e-06 PPR repeat
TPR_24 PF23276 288 - 399 7.1e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 296 - 351 1.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 305 - 351 5.4e-13 PPR repeat family
PPR_3 PF13812 327 - 387 1.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 340 - 386 1.2e-10 PPR repeat family
PPR_long PF17177 358 - 468 8e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 371 - 402 6.7e-06 PPR repeat
PPR_2 PF13041 373 - 421 3.4e-14 PPR repeat family
PPR_1 PF12854 406 - 436 9.1e-09 PPR repeat
PPR PF01535 412 - 441 1.8e-07 PPR repeat
PPR_2 PF13041 416 - 448 1.1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1367
Acc36I ACCTGC 1 cut(s) 1037
AciI CCGC 3 cut(s) 247, 503, 586
AclWI GGATC 3 cut(s) 95, 635, 1109
AcoI YGGCCR 1 cut(s) 1325
AcsI RAATTY 4 cut(s) 183, 496, 646, 1394
AfaI GTAC 2 cut(s) 341, 1389
AfiI CCNNNNNNNGG 2 cut(s) 660, 1023
AflIII ACRYGT 1 cut(s) 972
AjnI CCWGG 1 cut(s) 428
AluBI AGCT 3 cut(s) 21, 358, 1050
AluI AGCT 3 cut(s) 21, 358, 1050
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 1 cut(s) 392
AlwI GGATC 3 cut(s) 95, 635, 1109
AoxI GGCC 2 cut(s) 514, 1325
ApeKI GCWGC 2 cut(s) 263, 271
ApoI RAATTY 4 cut(s) 183, 496, 646, 1394
AseI ATTAAT 2 cut(s) 620, 1251
AspS9I GGNCC 3 cut(s) 375, 583, 1026
AsuHPI GGTGA 1 cut(s) 180
AvaII GGWCC 3 cut(s) 375, 583, 1026
BanII GRGCYC 1 cut(s) 436
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 275, 283
BccI CCATC 5 cut(s) 620, 624, 883, 1171, 1336
BceAI ACGGC 1 cut(s) 905
BciT130I CCWGG 1 cut(s) 430
BciVI GTATCC 1 cut(s) 378
BclI TGATCA 2 cut(s) 166, 1101
BcoDI GTCTC 1 cut(s) 392
BfaI CTAG 1 cut(s) 792
BfuAI ACCTGC 1 cut(s) 1037
BfuI GTATCC 1 cut(s) 378
BisI GCNGC 3 cut(s) 264, 272, 504
BlsI GCNGC 3 cut(s) 265, 273, 505
Bme1390I CCNGG 1 cut(s) 430
Bme18I GGWCC 3 cut(s) 375, 583, 1026
BmgT120I GGNCC 3 cut(s) 375, 583, 1026
BmiI GGNNCC 1 cut(s) 377
BmrFI CCNGG 1 cut(s) 430
BmrI ACTGGG 1 cut(s) 1218
BmsI GCATC 5 cut(s) 42, 676, 778, 856, 1111
BmuI ACTGGG 1 cut(s) 1218
BplI GAGNNNNNCTC 4 cut(s) 541, 573, 584, 616
Bpu10I CCTNAGC 1 cut(s) 558
Bsa29I ATCGAT 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 429, 654, 1060
Bsc4I CCNNNNNNNGG 2 cut(s) 660, 1023
Bse1I ACTGG 2 cut(s) 1224, 1302
BseBI CCWGG 1 cut(s) 430
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 429, 654, 1060
BseGI GGATG 4 cut(s) 54, 211, 635, 793
BseLI CCNNNNNNNGG 2 cut(s) 660, 1023
BseMII CTCAG 4 cut(s) 449, 512, 572, 1254
BseNI ACTGG 2 cut(s) 1224, 1302
BseRI GAGGAG 1 cut(s) 1409
BseXI GCAGC 2 cut(s) 275, 283
BshFI GGCC 2 cut(s) 516, 1327
BshVI ATCGAT 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 660, 1023
BsmAI GTCTC 1 cut(s) 392
BsmI GAATGC 3 cut(s) 232, 506, 878
BsnI GGCC 2 cut(s) 516, 1327
Bsp1286I GDGCHC 2 cut(s) 436, 493
Bsp143I GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
BspACI CCGC 3 cut(s) 247, 503, 586
BspANI GGCC 2 cut(s) 516, 1327
BspCNI CTCAG 4 cut(s) 448, 513, 571, 1253
BspDI ATCGAT 1 cut(s) 315
BspHI TCATGA 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 377
BspMI ACCTGC 1 cut(s) 1037
BspPI GGATC 3 cut(s) 95, 635, 1109
BsrI ACTGG 2 cut(s) 1224, 1302
BssECI CCNNGG 3 cut(s) 429, 654, 1060
BssMI GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 654
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 430
Bst4CI ACNGT 6 cut(s) 71, 614, 824, 1061, 1215, 1359
Bst6I CTCTTC 2 cut(s) 1296, 1372
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 691
BstDEI CTNAG 4 cut(s) 435, 521, 558, 1240
BstDSI CCRYGG 1 cut(s) 1060
BstF5I GGATG 4 cut(s) 54, 211, 635, 793
BstKTI GATC 7 cut(s) 103, 169, 315, 348, 643, 1104, 1117
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
BstMWI GCNNNNNNNGC 4 cut(s) 305, 875, 917, 1283
BstNI CCWGG 1 cut(s) 430
BstSCI CCNGG 1 cut(s) 428
BstV1I GCAGC 2 cut(s) 275, 283
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
Bsu15I ATCGAT 1 cut(s) 315
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 2 cut(s) 516, 1327
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 1060
BtsCI GGATG 4 cut(s) 54, 211, 635, 793
BtsIMutI CAGTG 1 cut(s) 829
BveI ACCTGC 1 cut(s) 1037
Cac8I GCNNGC 1 cut(s) 691
CciI TCATGA 1 cut(s) 126
Cfr13I GGNCC 3 cut(s) 375, 583, 1026
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 2 cut(s) 340, 1388
CviAII CATG 6 cut(s) 127, 305, 349, 724, 784, 1201
CviQI GTAC 2 cut(s) 340, 1388
DdeI CTNAG 4 cut(s) 435, 521, 558, 1240
DpnI GATC 7 cut(s) 102, 168, 314, 347, 642, 1103, 1116
DpnII GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
EaeI YGGCCR 1 cut(s) 1325
Eam1104I CTCTTC 2 cut(s) 1296, 1372
EarI CTCTTC 2 cut(s) 1296, 1372
Eco130I CCWWGG 1 cut(s) 654
Eco24I GRGCYC 1 cut(s) 436
Eco47I GGWCC 3 cut(s) 375, 583, 1026
EcoO109I RGGNCCY 2 cut(s) 375, 1026
EcoRII CCWGG 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 654
EcoT22I ATGCAT 1 cut(s) 1233
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 6 cut(s) 130, 308, 352, 727, 787, 1204
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FatI CATG 6 cut(s) 126, 304, 348, 723, 783, 1200
FauNDI CATATG 1 cut(s) 841
FbaI TGATCA 2 cut(s) 166, 1101
Fnu4HI GCNGC 3 cut(s) 264, 272, 504
FokI GGATG 4 cut(s) 61, 218, 642, 800
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 3 cut(s) 264, 272, 504
FspBI CTAG 1 cut(s) 792
GluI GCNGC 3 cut(s) 264, 272, 504
HaeIII GGCC 2 cut(s) 516, 1327
HapII CCGG 1 cut(s) 231
Hin1II CATG 6 cut(s) 130, 308, 352, 727, 787, 1204
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HinfI GANTC 5 cut(s) 219, 317, 599, 903, 1321
HpaI GTTAAC 1 cut(s) 958
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 3 cut(s) 364, 757, 958
Hpy188I TCNGA 4 cut(s) 182, 438, 495, 1441
Hpy8I GTNNAC 3 cut(s) 364, 757, 958
HpyAV CCTTC 5 cut(s) 433, 547, 574, 1309, 1367
HpyCH4III ACNGT 6 cut(s) 71, 614, 824, 1061, 1215, 1359
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 9 cut(s) 120, 242, 534, 741, 835, 1032, 1124, 1200, 1231
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 875, 917, 1283
HpyF3I CTNAG 4 cut(s) 435, 521, 558, 1240
HpySE526I ACGT 1 cut(s) 972
Hsp92II CATG 6 cut(s) 130, 308, 352, 727, 787, 1204
Ksp22I TGATCA 2 cut(s) 166, 1101
KspAI GTTAAC 1 cut(s) 958
Kzo9I GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
LmnI GCTCC 2 cut(s) 355, 496
Lsp1109I GCAGC 2 cut(s) 275, 283
LweI GCATC 5 cut(s) 42, 676, 778, 856, 1111
MaeI CTAG 1 cut(s) 792
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 3 cut(s) 709, 1009, 1336
MalI GATC 7 cut(s) 102, 168, 314, 347, 642, 1103, 1116
MboI GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
MboII GAAGA 5 cut(s) 875, 1313, 1389, 1391, 1394
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 436, 493
MluCI AATT 9 cut(s) 32, 64, 183, 496, 621, 646, 1018, 1169, 1394
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 128
Mph1103I ATGCAT 1 cut(s) 1233
MseI TTAA 9 cut(s) 294, 465, 620, 957, 986, 1043, 1185, 1251, 1352
MslI CAYNNNNRTG 1 cut(s) 840
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 430
Mva1269I GAATGC 3 cut(s) 232, 506, 878
MvaI CCWGG 1 cut(s) 430
MwoI GCNNNNNNNGC 4 cut(s) 305, 875, 917, 1283
NdeI CATATG 1 cut(s) 841
NdeII GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
NlaIII CATG 6 cut(s) 130, 308, 352, 727, 787, 1204
NlaIV GGNNCC 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 709, 1336
NsiI ATGCAT 1 cut(s) 1233
PagI TCATGA 1 cut(s) 126
PctI GAATGC 3 cut(s) 232, 506, 878
PfeI GAWTC 4 cut(s) 219, 317, 903, 1321
PkrI GCNGC 3 cut(s) 265, 273, 505
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
PpuMI RGGWCCY 2 cut(s) 375, 1026
PshBI ATTAAT 2 cut(s) 620, 1251
PsiI TTATAA 1 cut(s) 1367
Psp5II RGGWCCY 2 cut(s) 375, 1026
Psp6I CCWGG 1 cut(s) 428
PspGI CCWGG 1 cut(s) 428
PspN4I GGNNCC 1 cut(s) 377
PspPI GGNCC 3 cut(s) 375, 583, 1026
PspPPI RGGWCCY 2 cut(s) 375, 1026
PsrI GAACNNNNNNTAC 2 cut(s) 192, 224
PsuI RGATCY 1 cut(s) 640
RsaI GTAC 2 cut(s) 341, 1389
RsaNI GTAC 2 cut(s) 340, 1388
RseI CAYNNNNRTG 1 cut(s) 840
SaqAI TTAA 9 cut(s) 294, 465, 620, 957, 986, 1043, 1185, 1251, 1352
SatI GCNGC 3 cut(s) 264, 272, 504
Sau3AI GATC 7 cut(s) 100, 166, 312, 345, 640, 1101, 1114
Sau96I GGNCC 3 cut(s) 375, 583, 1026
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 1 cut(s) 430
SduI GDGCHC 2 cut(s) 436, 493
SfaNI GCATC 5 cut(s) 42, 676, 778, 856, 1111
SinI GGWCC 3 cut(s) 375, 583, 1026
SmiMI CAYNNNNRTG 1 cut(s) 840
Sse9I AATT 9 cut(s) 32, 64, 183, 496, 621, 646, 1018, 1169, 1394
SsiI CCGC 3 cut(s) 247, 503, 586
SspMI CTAG 1 cut(s) 792
StyD4I CCNGG 1 cut(s) 428
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 6 cut(s) 71, 614, 824, 1061, 1215, 1359
TaiI ACGT 1 cut(s) 975
TaqI TCGA 2 cut(s) 315, 1022
TasI AATT 9 cut(s) 32, 64, 183, 496, 621, 646, 1018, 1169, 1394
TatI WGTACW 1 cut(s) 1387
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 4 cut(s) 219, 317, 903, 1321
Tru1I TTAA 9 cut(s) 294, 465, 620, 957, 986, 1043, 1185, 1251, 1352
Tru9I TTAA 9 cut(s) 294, 465, 620, 957, 986, 1043, 1185, 1251, 1352
TscAI CASTG 1 cut(s) 829
TseFI GTSAC 2 cut(s) 709, 1336
TseI GCWGC 2 cut(s) 263, 271
Tsp45I GTSAC 2 cut(s) 709, 1336
TspDTI ATGAA 3 cut(s) 593, 1182, 1217
TspRI CASTG 1 cut(s) 829
VpaK11BI GGWCC 3 cut(s) 375, 583, 1026
VspI ATTAAT 2 cut(s) 620, 1251
XapI RAATTY 4 cut(s) 183, 496, 646, 1394
XspI CTAG 1 cut(s) 792
Zsp2I ATGCAT 1 cut(s) 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.