AT1G77380

amino acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004566
Physical Location & Seq
Reverse (-)
29074879 .. 29077390
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77380.1

Sequence Viewer

Length: 1431 bp
ATGGTTCAAAACCACCAAACAGTTCTCGCCGTCGATATGCCACAAACCGGCGGCTCCAAGTACTTGGACGACGACGGGAAAAACAAAAGAACTGGGAGTGTTTGGACGGCGAGTGCACACATAATAACGGCAGTGATAGGTTCGGGAGTTTTGTCACTAGCGTGGGCTACGGCGCAGCTAGGTTGGCTCGCCGGACCGGTGGTGATGTTGCTCTTCTCTGCCGTCACTTATTTCACTTCTTCTCTTCTTGCTGCTTGTTACCGCTCCGGCGACCCTATCTCCGGCAAGAGGAACTACACTTATATGGATGCTGTCCGATCAAATCTCGGTGGCGTGAAGGTGACGCTATGTGGGATTGTTCAGTATCTTAATATCTTTGGTGTTGCTATTGGCTACACAATTGCTTCAGCTATAAGCATGATGGCAATAAAGAGATCAAACTGTTTCCACAAGAGTGGAGGGAAAGATCCATGTCACATGAACAGTAATCCTTACATGATAGCTTTTGGATTAGTCCAGATTCTATTCTCTCAGATTCCAGATTTTGATCAACTTTGGTGGCTCTCAATCCTCGCCGCCGTTATGTCCTTCACTTATTCCTCAGCCGGTCTCGCCCTCGGCATAGCCCAAGTTGTCGTTAATGGGAAGGTGAAGGGAAGTCTCACTGGGATTAGCATAGGAGCAGTAACAGAGACACAGAAGATATGGAGGACCTTTCAAGCTCTTGGAGACATTGCTTTTGCTTACTCTTACTCCATTATCCTCATCGAGATTCAGGACACAGTGAAGTCACCACCATCAGAAGAGAAGACGATGAAGAAGGCAACACTTGTGAGCGTCAGTGTAACGACTATGTTCTATATGTTGTGTGGATGTATGGGATATGCAGCCTTTGGAGACTTGTCTCCGGGAAATCTCTTAACCGGTTTCGGGTTTTATAATCCTTATTGGCTTCTTGACATTGCAAATGCAGCCATTGTGATTCACCTTATTGGTGCATACCAAGTCTATTGCCAACCTCTGTTTGCTTTCATCGAGAAGCAAGCTTCCATTCAATTCCCTGATAGTGAGTTCATTGCAAAAGATATCAAAATTCCAATTCCTGGTTTCAAGCCTCTCCGCTTGAATGTCTTCAGGTTGATATGGAGGACAGTGTTTGTGATCATAACGACAGTTATCTCAATGCTTCTTCCGTTTTTCAACGACGTTGTGGGTCTGCTCGGGGCACTAGGGTTTTGGCCATTGACGGTGTATTTTCCAGTGGAAATGTACATCGCGCAGAAGAAGATACCTAGATGGAGCACCAGATGGGTTTGCCTTCAAGTCTTCAGCTTAGGGTGTCTAGTAGTTAGCATTGCTGCAGCTGCAGGGTCCATAGCTGGAGTACTTCTTGATCTAAAGTCCTACAAGCCATTTCGAAGCGAATACTGA

Protein Analysis

476

Amino Acids

52.04

Weight (kDa)

9.04

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 30 - 463 4.6e-124 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 939
AccB7I CCANNNNNTGG 1 cut(s) 1103
AccBSI CCGCTC 1 cut(s) 264
AccII CGCG 1 cut(s) 1277
AciI CCGC 4 cut(s) 51, 262, 576, 1120
AclWI GGATC 1 cut(s) 461
AcoI YGGCCR 1 cut(s) 1238
AcsI RAATTY 1 cut(s) 1092
AcuI CTGAAG 3 cut(s) 390, 1117, 1312
AfaI GTAC 3 cut(s) 62, 1271, 1386
AfiI CCNNNNNNNGG 5 cut(s) 47, 281, 288, 930, 1103
AgeI ACCGGT 2 cut(s) 196, 923
AgsI TTSAA 7 cut(s) 8, 719, 1055, 1111, 1126, 1201, 1322
AjnI CCWGG 1 cut(s) 1102
AleI CACNNNNGTG 2 cut(s) 160, 453
AluBI AGCT 8 cut(s) 178, 410, 503, 722, 1046, 1332, 1364, 1379
AluI AGCT 8 cut(s) 178, 410, 503, 722, 1046, 1332, 1364, 1379
Alw21I GWGCWC 2 cut(s) 118, 1304
Alw26I GTCTC 6 cut(s) 614, 665, 686, 723, 891, 909
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 1220
AoxI GGCC 1 cut(s) 1238
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 7 cut(s) 175, 251, 887, 971, 1358, 1361, 1364
ApoI RAATTY 1 cut(s) 1092
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AsiGI ACCGGT 2 cut(s) 196, 923
Asp700I GAANNNNTTC 1 cut(s) 1130
AspLEI GCGC 2 cut(s) 175, 1279
AspS9I GGNCC 3 cut(s) 194, 711, 1371
AsuC2I CCSGG 1 cut(s) 909
AsuHPI GGTGA 5 cut(s) 214, 352, 661, 783, 977
AsuII TTCGAA 1 cut(s) 1417
AvaI CYCGRG 1 cut(s) 1220
AvaII GGWCC 3 cut(s) 194, 711, 1371
BaeGI GKGCMC 2 cut(s) 118, 1228
BalI TGGCCA 1 cut(s) 1240
BbsI GAAGAC 3 cut(s) 815, 1123, 1318
Bbv12I GWGCWC 2 cut(s) 118, 1304
BbvCI CCTCAGC 1 cut(s) 601
BbvI GCAGC 7 cut(s) 187, 238, 899, 983, 1345, 1351, 1373
BccI CCATC 4 cut(s) 415, 805, 1290, 1302
BceAI ACGGC 6 cut(s) 14, 123, 144, 186, 206, 563
BciT130I CCWGG 1 cut(s) 1104
BclI TGATCA 2 cut(s) 547, 1161
BcnI CCSGG 1 cut(s) 909
BcoDI GTCTC 6 cut(s) 614, 665, 686, 723, 891, 909
BfaI CTAG 5 cut(s) 158, 179, 1229, 1293, 1343
BfmI CTRYAG 2 cut(s) 1359, 1365
BisI GCNGC 9 cut(s) 52, 176, 252, 576, 888, 972, 1359, 1362, 1365
BlsI GCNGC 9 cut(s) 53, 177, 253, 577, 889, 973, 1360, 1363, 1366
BmcAI AGTACT 2 cut(s) 62, 1386
Bme1390I CCNGG 2 cut(s) 909, 1104
Bme18I GGWCC 3 cut(s) 194, 711, 1371
BmeT110I CYCGRG 1 cut(s) 1220
BmgT120I GGNCC 3 cut(s) 194, 711, 1371
BmiI GGNNCC 2 cut(s) 55, 1372
BmrFI CCNGG 2 cut(s) 909, 1104
BmrI ACTGGG 2 cut(s) 102, 675
BmsI GCATC 1 cut(s) 298
BmuI ACTGGG 2 cut(s) 102, 675
BpiI GAAGAC 3 cut(s) 815, 1123, 1318
BpmI CTGGAG 1 cut(s) 1401
Bpu10I CCTNAGC 2 cut(s) 601, 1333
Bpu14I TTCGAA 1 cut(s) 1417
BpuMI CCSGG 1 cut(s) 909
BsaBI GATNNNNATC 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 614
BsaJI CCNNGG 1 cut(s) 616
BsaWI WCCGGW 2 cut(s) 196, 923
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 737, 767
Bsc4I CCNNNNNNNGG 5 cut(s) 47, 281, 288, 930, 1103
Bse118I RCCGGY 4 cut(s) 47, 196, 605, 923
Bse1I ACTGG 3 cut(s) 97, 670, 1259
Bse3DI GCAATG 4 cut(s) 732, 960, 1074, 1353
Bse8I GATNNNNATC 1 cut(s) 546
BseBI CCWGG 1 cut(s) 1104
BseDI CCNNGG 1 cut(s) 616
BseGI GGATG 2 cut(s) 313, 878
BseJI GATNNNNATC 1 cut(s) 546
BseLI CCNNNNNNNGG 5 cut(s) 47, 281, 288, 930, 1103
BseMI GCAATG 4 cut(s) 732, 960, 1074, 1353
BseMII CTCAG 2 cut(s) 545, 615
BseNI ACTGG 3 cut(s) 97, 670, 1259
BseSI GKGCMC 2 cut(s) 118, 1228
BseXI GCAGC 7 cut(s) 187, 238, 899, 983, 1345, 1351, 1373
Bsh1236I CGCG 1 cut(s) 1277
BshFI GGCC 1 cut(s) 1240
BshTI ACCGGT 2 cut(s) 196, 923
BsiHKAI GWGCWC 2 cut(s) 118, 1304
BsiHKCI CYCGRG 1 cut(s) 1220
BsiSI CCGG 8 cut(s) 48, 192, 197, 267, 282, 606, 908, 924
BslI CCNNNNNNNGG 5 cut(s) 47, 281, 288, 930, 1103
BsmAI GTCTC 6 cut(s) 614, 665, 686, 723, 891, 909
BsnI GGCC 1 cut(s) 1240
Bso31I GGTCTC 1 cut(s) 614
BsoBI CYCGRG 1 cut(s) 1220
Bsp119I TTCGAA 1 cut(s) 1417
Bsp1286I GDGCHC 3 cut(s) 118, 1228, 1304
Bsp1407I TGTACA 1 cut(s) 1269
Bsp143I GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
BspACI CCGC 4 cut(s) 51, 262, 576, 1120
BspANI GGCC 1 cut(s) 1240
BspCNI CTCAG 2 cut(s) 544, 614
BspFNI CGCG 1 cut(s) 1277
BspLI GGNNCC 2 cut(s) 55, 1372
BspMAI CTGCAG 2 cut(s) 1363, 1369
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 218
BspT104I TTCGAA 1 cut(s) 1417
BspTNI GGTCTC 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 264
BsrDI GCAATG 4 cut(s) 732, 960, 1074, 1353
BsrFI RCCGGY 4 cut(s) 47, 196, 605, 923
BsrGI TGTACA 1 cut(s) 1269
BsrI ACTGG 3 cut(s) 97, 670, 1259
BssAI RCCGGY 4 cut(s) 47, 196, 605, 923
BssECI CCNNGG 1 cut(s) 616
BssMI GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
Bst2UI CCWGG 1 cut(s) 1104
Bst4CI ACNGT 7 cut(s) 22, 443, 485, 784, 1153, 1174, 1249
Bst6I CTCTTC 3 cut(s) 218, 249, 798
BstAUI TGTACA 1 cut(s) 1269
BstBI TTCGAA 1 cut(s) 1417
BstC8I GCNNGC 2 cut(s) 189, 1044
BstDEI CTNAG 3 cut(s) 531, 601, 1333
BstF5I GGATG 2 cut(s) 313, 878
BstFNI CGCG 1 cut(s) 1277
BstHHI GCGC 2 cut(s) 175, 1279
BstKTI GATC 6 cut(s) 320, 437, 469, 550, 1164, 1396
BstMAI GTCTC 6 cut(s) 614, 665, 686, 723, 891, 909
BstMBI GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
BstMWI GCNNNNNNNGC 4 cut(s) 184, 611, 971, 1364
BstNI CCWGG 1 cut(s) 1104
BstSCI CCNGG 2 cut(s) 907, 1102
BstSFI CTRYAG 2 cut(s) 1359, 1365
BstSLI GKGCMC 2 cut(s) 118, 1228
BstUI CGCG 1 cut(s) 1277
BstV1I GCAGC 7 cut(s) 187, 238, 899, 983, 1345, 1351, 1373
BstV2I GAAGAC 3 cut(s) 815, 1123, 1318
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 2 cut(s) 64, 455
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 1 cut(s) 1240
BtgZI GCGATG 1 cut(s) 1258
BtsCI GGATG 2 cut(s) 313, 878
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 6 cut(s) 138, 663, 789, 847, 1158, 1266
Cac8I GCNNGC 2 cut(s) 189, 1044
CfoI GCGC 2 cut(s) 175, 1279
Cfr10I RCCGGY 4 cut(s) 47, 196, 605, 923
Cfr13I GGNCC 3 cut(s) 194, 711, 1371
CpoI CGGWCCG 1 cut(s) 194
CseI GACGC 2 cut(s) 352, 826
Csp6I GTAC 3 cut(s) 61, 1270, 1385
CspAI ACCGGT 2 cut(s) 196, 923
CspCI CAANNNNNGTGG 2 cut(s) 539, 574
CspI CGGWCCG 1 cut(s) 194
CviAII CATG 4 cut(s) 418, 471, 478, 496
CviQI GTAC 3 cut(s) 61, 1270, 1385
DdeI CTNAG 3 cut(s) 531, 601, 1333
DpnI GATC 6 cut(s) 319, 436, 468, 549, 1163, 1395
DpnII GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
EaeI YGGCCR 1 cut(s) 1238
Eam1104I CTCTTC 3 cut(s) 218, 249, 798
EarI CTCTTC 3 cut(s) 218, 249, 798
Eco31I GGTCTC 1 cut(s) 614
Eco32I GATATC 1 cut(s) 1087
Eco47I GGWCC 3 cut(s) 194, 711, 1371
Eco57I CTGAAG 3 cut(s) 390, 1117, 1312
Eco88I CYCGRG 1 cut(s) 1220
EcoO109I RGGNCCY 1 cut(s) 711
EcoRII CCWGG 1 cut(s) 1102
EcoRV GATATC 1 cut(s) 1087
FaeI CATG 4 cut(s) 421, 474, 481, 499
FatI CATG 4 cut(s) 417, 470, 477, 495
FbaI TGATCA 2 cut(s) 547, 1161
Fnu4HI GCNGC 9 cut(s) 52, 176, 252, 576, 888, 972, 1359, 1362, 1365
FokI GGATG 2 cut(s) 320, 885
Fsp4HI GCNGC 9 cut(s) 52, 176, 252, 576, 888, 972, 1359, 1362, 1365
FspBI CTAG 5 cut(s) 158, 179, 1229, 1293, 1343
GlaI GCGC 2 cut(s) 174, 1278
GluI GCNGC 9 cut(s) 52, 176, 252, 576, 888, 972, 1359, 1362, 1365
GsuI CTGGAG 1 cut(s) 1401
HaeIII GGCC 1 cut(s) 1240
HapII CCGG 8 cut(s) 48, 192, 197, 267, 282, 606, 908, 924
HgaI GACGC 2 cut(s) 352, 826
HhaI GCGC 2 cut(s) 175, 1279
Hin1II CATG 4 cut(s) 421, 474, 481, 499
Hin6I GCGC 2 cut(s) 173, 1277
HinP1I GCGC 2 cut(s) 173, 1277
HindIII AAGCTT 1 cut(s) 1044
HinfI GANTC 4 cut(s) 520, 535, 772, 982
HpaII CCGG 8 cut(s) 48, 192, 197, 267, 282, 606, 908, 924
HphI GGTGA 5 cut(s) 214, 352, 661, 783, 977
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 317, 534, 802
Hpy188III TCNNGA 8 cut(s) 144, 517, 539, 769, 776, 956, 1036, 1391
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 4 cut(s) 35, 74, 77, 1208
HpyAV CCTTC 6 cut(s) 331, 598, 640, 646, 814, 1328
HpyCH4III ACNGT 7 cut(s) 22, 443, 485, 784, 1153, 1174, 1249
HpyCH4IV ACGT 1 cut(s) 1206
HpyCH4V TGCA 8 cut(s) 116, 887, 965, 971, 998, 1079, 1361, 1367
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 611, 971, 1364
HpyF3I CTNAG 3 cut(s) 531, 601, 1333
HpySE526I ACGT 1 cut(s) 1206
Hsp92II CATG 4 cut(s) 421, 474, 481, 499
HspAI GCGC 2 cut(s) 173, 1277
Ksp22I TGATCA 2 cut(s) 547, 1161
Kzo9I GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 4 cut(s) 59, 269, 680, 1299
Lsp1109I GCAGC 7 cut(s) 187, 238, 899, 983, 1345, 1351, 1373
LweI GCATC 1 cut(s) 298
MaeI CTAG 5 cut(s) 158, 179, 1229, 1293, 1343
MaeII ACGT 1 cut(s) 1206
MaeIII GTNAC 8 cut(s) 153, 223, 257, 340, 473, 685, 789, 844
MalI GATC 6 cut(s) 319, 436, 468, 549, 1163, 1395
MbiI CCGCTC 1 cut(s) 264
MboI GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
MfeI CAATTG 1 cut(s) 399
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 3 cut(s) 118, 1228, 1304
MlsI TGGCCA 1 cut(s) 1240
MluCI AATT 4 cut(s) 399, 1055, 1092, 1098
MluNI TGGCCA 1 cut(s) 1240
Mox20I TGGCCA 1 cut(s) 1240
MroXI GAANNNNTTC 1 cut(s) 1130
MscI TGGCCA 1 cut(s) 1240
MseI TTAA 3 cut(s) 369, 639, 920
MslI CAYNNNNRTG 3 cut(s) 160, 302, 453
Msp20I TGGCCA 1 cut(s) 1240
MspA1I CMGCKG 1 cut(s) 1364
MspI CCGG 8 cut(s) 48, 192, 197, 267, 282, 606, 908, 924
MspR9I CCNGG 2 cut(s) 909, 1104
MunI CAATTG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 1104
MvnI CGCG 1 cut(s) 1277
MwoI GCNNNNNNNGC 4 cut(s) 184, 611, 971, 1364
NciI CCSGG 1 cut(s) 909
NdeII GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
NlaIII CATG 4 cut(s) 421, 474, 481, 499
NlaIV GGNNCC 2 cut(s) 55, 1372
NmeAIII GCCGAG 1 cut(s) 597
NmuCI GTSAC 5 cut(s) 153, 223, 340, 473, 789
NspV TTCGAA 1 cut(s) 1417
OliI CACNNNNGTG 2 cut(s) 160, 453
PciSI GCTCTTC 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 1130
PfeI GAWTC 4 cut(s) 520, 535, 772, 982
PflMI CCANNNNNTGG 1 cut(s) 1103
PfoI TCCNGGA 1 cut(s) 907
PinAI ACCGGT 2 cut(s) 196, 923
PkrI GCNGC 9 cut(s) 53, 177, 253, 577, 889, 973, 1360, 1363, 1366
PpuMI RGGWCCY 1 cut(s) 711
PsiI TTATAA 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 1102
PspGI CCWGG 1 cut(s) 1102
PspN4I GGNNCC 2 cut(s) 55, 1372
PspPI GGNCC 3 cut(s) 194, 711, 1371
PspPPI RGGWCCY 1 cut(s) 711
PstI CTGCAG 2 cut(s) 1363, 1369
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 3 cut(s) 62, 1271, 1386
RsaNI GTAC 3 cut(s) 61, 1270, 1385
RseI CAYNNNNRTG 3 cut(s) 160, 302, 453
Rsr2I CGGWCCG 1 cut(s) 194
RsrII CGGWCCG 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 3 cut(s) 369, 639, 920
SatI GCNGC 9 cut(s) 52, 176, 252, 576, 888, 972, 1359, 1362, 1365
Sau3AI GATC 6 cut(s) 317, 434, 466, 547, 1161, 1393
Sau96I GGNCC 3 cut(s) 194, 711, 1371
ScaI AGTACT 2 cut(s) 62, 1386
ScrFI CCNGG 2 cut(s) 909, 1104
SduI GDGCHC 3 cut(s) 118, 1228, 1304
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 2 cut(s) 1359, 1365
SfuI TTCGAA 1 cut(s) 1417
SinI GGWCC 3 cut(s) 194, 711, 1371
SmiMI CAYNNNNRTG 3 cut(s) 160, 302, 453
Sse9I AATT 4 cut(s) 399, 1055, 1092, 1098
SsiI CCGC 4 cut(s) 51, 262, 576, 1120
SspMI CTAG 5 cut(s) 158, 179, 1229, 1293, 1343
StyD4I CCNGG 2 cut(s) 907, 1102
TaaI ACNGT 7 cut(s) 22, 443, 485, 784, 1153, 1174, 1249
TaiI ACGT 1 cut(s) 1209
TaqI TCGA 4 cut(s) 33, 768, 1035, 1417
TasI AATT 4 cut(s) 399, 1055, 1092, 1098
TatI WGTACW 3 cut(s) 60, 1269, 1384
TauI GCSGC 2 cut(s) 54, 578
TfiI GAWTC 4 cut(s) 520, 535, 772, 982
Tru1I TTAA 3 cut(s) 369, 639, 920
Tru9I TTAA 3 cut(s) 369, 639, 920
TscAI CASTG 6 cut(s) 138, 670, 789, 847, 1158, 1266
TseFI GTSAC 5 cut(s) 153, 223, 340, 473, 789
TseI GCWGC 7 cut(s) 175, 251, 887, 971, 1358, 1361, 1364
Tsp45I GTSAC 5 cut(s) 153, 223, 340, 473, 789
TspDTI ATGAA 4 cut(s) 494, 830, 1021, 1063
TspGWI ACGGA 1 cut(s) 1182
TspRI CASTG 6 cut(s) 138, 670, 789, 847, 1158, 1266
Van91I CCANNNNNTGG 1 cut(s) 1103
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 3 cut(s) 194, 711, 1371
XapI RAATTY 1 cut(s) 1092
XmnI GAANNNNTTC 1 cut(s) 1130
XspI CTAG 5 cut(s) 158, 179, 1229, 1293, 1343
ZrmI AGTACT 2 cut(s) 62, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.